RchiOBHm_Chr5g0018731

Di-glucose binding within endoplasmic reticulum

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000088
Physical Location & Seq
Forward (+)
13207623 .. 13215498
7876 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29889

Sequence Viewer

Length: 3129 bp
ATGAAGCATTCTTCTCTTTCATTACTATGCTTCTGCCTAGGCTATCTGGGTAGTTTCTGGTTTCACGTTTCCATGGCTCAAAATGCTACTAATGCTACTACGGACCCATCTGAAGGTAGTATTCAACTATATATCTTCTTTGCTTCTGGTTCAGTCAATAAAAGTGACCAACTTAATTCGAGCCTTAATATGTGTGTATATTTTTCAGTGTCGGCTTTGAACTCAATCTTCCAACAGTGGGGAGCACAGTCGGTGGCGCTGTGGAATATCAGTGGAGAGCCATGCAGTGGATCAGCCATCAATGGAACTGCATTTGAGAGCACTCCTAATAACCCTGCCATTACATGTGATTGTACCTTTGACAATAGTACCACCTGCCATATTACCCAACTGAGAGTTTATGCTCTAGACAAAACAGGAGGGTTTCCAGAAGAGCTTCTGGCTTTGAAATATCTGAAGTTTTTGAAAATAGATCAGAACTTTTTCACAGGCCCCTTACCCGCATGGATTGGCAATATGTCTGCATTGACGCTGTTGTCAATTGCCCACAATGCATTCTCTGGGCCGATCCCCAAGGAACTTGGAAATCTGAAGGACCTAAATACGCTGTCCTTTGGATCAAATAATTTCTCTGGAACACTCCCTCCAGAACTTGGTAATCTAATCAAGCTCCAGCAACTTTACATAGACAGCTGTGGACTTACTGGTGAAATTCCTTCAACATTTGCTAACCTTCGTAACATGCAAGTCCTTTGGGCATCAGGCACTCCTTTCTCTGGAAAGATACCTGATTTCATTGGGAATTGGACGAAGCTAAACTCACTGCGAATCAGTGATATATACAATGGGAGCTCCTCTCTTGGTTTCATAAAAAATTTGAAGAACTTGACTGATTTAGTTCTACGAAATGCATTGATTACTGGTAGCCTACCTTCTGATATTGCAGAATATCCAAGATTACAGATACTGGATTTGAGTTTCAACAATTTGACAGGCCAACTCCCAAATGGCTTGTTTAACATGAGTTCTCTTACATACTTAGACTTGTCTTACAACTATTTATCAGGGAGTCTTCCTCCATGGGTAACCTCAACAGCGCAACTTCTTCCTGGGTTGAATTGCCTTCAGAGAAATTTTCCATGCAATAGAAATGCCCCACGATATGCAAATTTTTCAATCAAGTGTGGTGGACAAGAACTTAGAGGAAGTGATGGTATATTGTATGAGGCAGAAAACTCAACTCTTGGCCCAGCTACATTTAGTGTAACCAGTACAGAGAAATGGGCGGTAAGCAATGCGGGTTTGTTTGCTGACAGAAAGAATCCAGACTATGTGGTGAATACCCTGGCACAAGTCACTGGGACAGATGTGACCCCAGAGCTTTTCCAGACCTCAAGATTGTCTCCCGGATCGCTGAGGTACTTCGGCCTGGGTCTGGAGAATGGGCCTTATACTGTAACATTGCAATTTGCAGAAACTGTTTTTTTAGATCGTACCTTACAAACATGGAAAAGTCTTGGTCGGCGCCTATTTGATATCTACATTCAGGGGGATCTAAAATGGAAGGACTTTGACATCTCGAAGGAGGCAGGTGGAGTTTTCAGAGCTGTTGCTAGAAACTTCAATGTTAATGTGTCAGAAAATTATCTTGACATTCATCTGTTCTGGGCTGGTAAGGGGACCTGCTGCATACCCGAACAAGGTGATTATGGCCCTCTAATATCAGCAGTTAAAGTTGTTCCAGAATTTACTCCAACTGTTTCTGGGATTCCACCAACTACTCCTGGAAAGAAGAGCAAGACAGGACTAATAGTTGGTATTGCAGTTCCTCTTGGAGTTGTGGGCTTGCTGTTAATATTTTCAGTTCTATATTTGAGGAGGAAAAAAGCAGAAAAATATGACGATGAAGGTAATAAGATATTTATGTGGTCGTTTCTGATGTTTGAGAAACATGGGACCTTGATATGTAGTGCAGAGAAAATACTTCTTATGCATTGTGAGACTAGCAACCTTCTAGCACTAGAGAACAGACCAAATACTTTCAGTTATTCCGAGTTGAAAGAAGCAACAGCATATTTTAATCCTTCAAATAAGCTAGGAGAGGGAGGATATGGCCCTGTTTACAAGGGTACACTTTCCGATGGGAGGGTAGTGGCTGTGAAGCAACTTTCAGTGGCATCTCACCAAGGGAAGAGTCAATTTGGTGCAGAAATTGCTACTATATCTGCAGTGCAACATCGCAATCTAGTGAAATTGTATGGATGCTGTATCGAAGGAAGCCAGCGAATTTTGGTTTATGAATATCTTGAAAACAAGAGCCTTGATCAAGCACTTTTTGGAACAAATGACCTGCACCTTGACTGGCTTACACGCTTCAATATAATGTTGGGAACAGCAAGAGGACTTGCTTATCTTCATGAGGAGTCAAGCCCAAGGATTGTGCATAGAGATGTCAAAGCCAGTAATATTTTGCTCGACACAGAACTCTGCCCAAAAATATCAGATTTTGGATTAGCAAAGCTATATGATGATGACAAAACCCATATCAGTACCCGAGTTGCAGGGACAATAGGCTATTTGGCACCAGAGTATGCAATGCGTGGACATCTGACCGAAAAGGCTGATGTTTTCAGTTTTGGGGTTGTTGTTTTGGAGATTCTCAGCGGAAGACCAAATGCTGACAGTGATTTGGATGCTGGAAAGACTTATCTCCTTGAATGGGCTTGGACTCTGCATGAAAATAACAAGACTTTGGAGTTGGTGGATCCCAGATTGACAGATTTTGATGATCAAGAAGCCATTAGGTTCATAAAAGTAGCTTTCCAATGCACACAGGGACCGCCAATGACGCGGCCATCTATGTCACGCGTGGTTGGGATGCTTTCTGGAGATATTGAAGTAGGCAGTACTGTCATGTCAAAGCCAAGTTATTTGACAGATTGGGACTATAAAGATGTAACAACAAGTAGCTTTTTGATTGATCGCGATACACCATCAACTGATAGTAAGAGTGAGCTCAACCTTCAGCCCGGCAGCACAACTGGTGCTAGCACCGATATTGACCTTTATCCATCTCATGTGGAAGTTAATCGATCAATGGTCACCGACATTATAAGAGAAGGAAGGTAA

Protein Analysis

1042

Amino Acids

114.68

Weight (kDa)

5.65

Isoelectric Point (pI)

29.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 213 - 375 6.2e-12 Leucine-rich repeat region
LRR_8 PF13855 318 - 356 6e-06 Leucine rich repeat
Malectin PF11721 391 - 577 3.7e-37 Malectin domain
Pkinase PF00069 695 - 957 6.4e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 696 - 958 2.6e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3113
AarI CACCTGC 2 cut(s) 383, 1580
AasI GACNNNNNNGTC 1 cut(s) 535
Acc36I ACCTGC 4 cut(s) 383, 1580, 1691, 2358
AccB1I GGYRCC 2 cut(s) 1524, 2581
AccB7I CCANNNNNTGG 2 cut(s) 287, 653
AccII CGCG 3 cut(s) 2851, 2868, 2985
AciI CCGC 6 cut(s) 501, 1286, 1298, 2664, 2840, 2851
AclWI GGATC 7 cut(s) 298, 562, 625, 1417, 1560, 2759, 2772
AcoI YGGCCR 1 cut(s) 2852
AcsI RAATTY 6 cut(s) 711, 874, 1132, 1168, 1745, 2286
AcuI CTGAAG 5 cut(s) 132, 476, 611, 1109, 3008
AcyI GRCGYC 1 cut(s) 1525
AfaI GTAC 8 cut(s) 355, 370, 1273, 1421, 1495, 2131, 2551, 2908
AfiI CCNNNNNNNGG 9 cut(s) 113, 238, 287, 653, 776, 1435, 1700, 2145, 2719
AflIII ACRYGT 2 cut(s) 344, 2866
AjnI CCWGG 4 cut(s) 1108, 1344, 1428, 1783
AloI GAACNNNNNNTCC 2 cut(s) 628, 660
Alw21I GWGCWC 4 cut(s) 247, 323, 854, 3018
Alw26I GTCTC 2 cut(s) 1407, 1994
AlwI GGATC 7 cut(s) 298, 562, 625, 1417, 1560, 2759, 2772
AlwNI CAGNNNCTG 2 cut(s) 967, 1478
Ama87I CYCGRG 1 cut(s) 2553
AoxI GGCC 9 cut(s) 490, 563, 994, 1246, 1426, 1445, 1711, 2113, 2852
ApeKI GCWGC 2 cut(s) 1686, 3033
ApoI RAATTY 6 cut(s) 711, 874, 1132, 1168, 1745, 2286
Asp700I GAANNNNTTC 2 cut(s) 435, 482
AspA2I CCTAGG 1 cut(s) 37
AspLEI GCGC 3 cut(s) 259, 1099, 1527
AsuC2I CCSGG 2 cut(s) 1407, 3030
AsuHPI GGTGA 5 cut(s) 719, 1348, 1715, 2174, 3094
AsuNHI GCTAGC 1 cut(s) 3047
AvaI CYCGRG 1 cut(s) 2553
AvaII GGWCC 5 cut(s) 103, 595, 1680, 1956, 2837
AvrII CCTAGG 1 cut(s) 37
BaeI ACNNNNGTAYC 2 cut(s) 352, 385
BamHI GGATCC 1 cut(s) 2764
BanI GGYRCC 2 cut(s) 1524, 2581
BanII GRGCYC 2 cut(s) 854, 3018
BarI GAAGNNNNNNTAC 2 cut(s) 916, 948
BbsI GAAGAC 2 cut(s) 1064, 2674
Bbv12I GWGCWC 4 cut(s) 247, 323, 854, 3018
BbvCI CCTCAGC 1 cut(s) 1415
BbvI GCAGC 2 cut(s) 1673, 3045
BccI CCATC 7 cut(s) 115, 305, 1205, 2135, 2863, 3001, 3079
BciT130I CCWGG 4 cut(s) 1110, 1346, 1430, 1785
BclI TGATCA 2 cut(s) 2323, 2788
BcnI CCSGG 2 cut(s) 1407, 3030
BcoDI GTCTC 2 cut(s) 1407, 1994
BfaI CTAG 9 cut(s) 38, 407, 1614, 2004, 2015, 2021, 2096, 2246, 3048
BfmI CTRYAG 1 cut(s) 2226
BfoI RGCGCY 2 cut(s) 260, 1528
BfuAI ACCTGC 4 cut(s) 383, 1580, 1691, 2358
BisI GCNGC 3 cut(s) 1687, 2852, 3034
BlnI CCTAGG 1 cut(s) 37
BlsI GCNGC 3 cut(s) 1688, 2853, 3035
BmcAI AGTACT 1 cut(s) 2908
Bme1390I CCNGG 6 cut(s) 1110, 1346, 1407, 1430, 1785, 3030
Bme18I GGWCC 5 cut(s) 103, 595, 1680, 1956, 2837
BmeT110I CYCGRG 1 cut(s) 2553
BmiI GGNNCC 8 cut(s) 105, 493, 1526, 1681, 1957, 2583, 2766, 2838
BmrFI CCNGG 6 cut(s) 1110, 1346, 1407, 1430, 1785, 3030
BmrI ACTGGG 1 cut(s) 1368
BmsI GCATC 5 cut(s) 767, 2186, 2252, 2683, 2868
BmtI GCTAGC 1 cut(s) 3051
BmuI ACTGGG 1 cut(s) 1368
BpiI GAAGAC 2 cut(s) 1064, 2674
BplI GAGNNNNNCTC 4 cut(s) 841, 873, 1060, 1092
BpmI CTGGAG 4 cut(s) 630, 656, 1457, 2907
Bpu10I CCTNAGC 1 cut(s) 1415
BpuEI CTTGAG 1 cut(s) 1378
BpuMI CCSGG 2 cut(s) 1407, 3030
Bsa29I ATCGAT 1 cut(s) 3091
BsaHI GRCGYC 1 cut(s) 1525
BsaJI CCNNGG 9 cut(s) 37, 72, 573, 1079, 1109, 1344, 1429, 2185, 2432
BsaXI ACNNNNNCTCC 2 cut(s) 628, 658
Bsc4I CCNNNNNNNGG 9 cut(s) 113, 238, 287, 653, 776, 1435, 1700, 2145, 2719
Bse1I ACTGG 8 cut(s) 709, 925, 972, 1269, 1363, 2366, 2460, 3046
Bse3DI GCAATG 3 cut(s) 1300, 1460, 2601
BseBI CCWGG 4 cut(s) 1110, 1346, 1430, 1785
BseCI ATCGAT 1 cut(s) 3091
BseDI CCNNGG 9 cut(s) 37, 72, 573, 1079, 1109, 1344, 1429, 2185, 2432
BseGI GGATG 3 cut(s) 2267, 2698, 2883
BseLI CCNNNNNNNGG 9 cut(s) 113, 238, 287, 653, 776, 1435, 1700, 2145, 2719
BseMI GCAATG 3 cut(s) 1300, 1460, 2601
BseMII CTCAG 3 cut(s) 383, 1406, 2674
BseNI ACTGG 8 cut(s) 709, 925, 972, 1269, 1363, 2366, 2460, 3046
BseRI GAGGAG 3 cut(s) 844, 1891, 2435
BseXI GCAGC 2 cut(s) 1673, 3045
BseYI CCCAGC 1 cut(s) 1249
BsgI GTGCAG 3 cut(s) 1992, 2226, 2336
Bsh1236I CGCG 3 cut(s) 2851, 2868, 2985
BshFI GGCC 9 cut(s) 492, 565, 996, 1248, 1428, 1447, 1713, 2115, 2854
BshNI GGYRCC 2 cut(s) 1524, 2581
BshVI ATCGAT 1 cut(s) 3091
BsiHKAI GWGCWC 4 cut(s) 247, 323, 854, 3018
BsiHKCI CYCGRG 1 cut(s) 2553
BsiSI CCGG 2 cut(s) 1407, 3030
BslFI GGGAC 6 cut(s) 1375, 1693, 1969, 2578, 2850, 2957
BslI CCNNNNNNNGG 9 cut(s) 113, 238, 287, 653, 776, 1435, 1700, 2145, 2719
BsmAI GTCTC 2 cut(s) 1407, 1994
BsmFI GGGAC 6 cut(s) 1375, 1693, 1969, 2578, 2850, 2957
BsmI GAATGC 2 cut(s) 7, 554
BsnI GGCC 9 cut(s) 492, 565, 996, 1248, 1428, 1447, 1713, 2115, 2854
BsoBI CYCGRG 1 cut(s) 2553
Bsp1286I GDGCHC 4 cut(s) 247, 323, 854, 3018
Bsp19I CCATGG 2 cut(s) 72, 1079
Bsp68I TCGCGA 1 cut(s) 2985
BspACI CCGC 6 cut(s) 501, 1286, 1298, 2664, 2840, 2851
BspANI GGCC 9 cut(s) 492, 565, 996, 1248, 1428, 1447, 1713, 2115, 2854
BspCNI CTCAG 3 cut(s) 384, 1407, 2673
BspDI ATCGAT 1 cut(s) 3091
BspFNI CGCG 3 cut(s) 2851, 2868, 2985
BspHI TCATGA 1 cut(s) 2416
BspLI GGNNCC 8 cut(s) 105, 493, 1526, 1681, 1957, 2583, 2766, 2838
BspMAI CTGCAG 1 cut(s) 2230
BspMI ACCTGC 4 cut(s) 383, 1580, 1691, 2358
BspOI GCTAGC 1 cut(s) 3051
BspPI GGATC 7 cut(s) 298, 562, 625, 1417, 1560, 2759, 2772
BspQI GCTCTTC 2 cut(s) 426, 1787
BspT107I GGYRCC 2 cut(s) 1524, 2581
BsrDI GCAATG 3 cut(s) 1300, 1460, 2601
BsrI ACTGG 8 cut(s) 709, 925, 972, 1269, 1363, 2366, 2460, 3046
BssECI CCNNGG 9 cut(s) 37, 72, 573, 1079, 1109, 1344, 1429, 2185, 2432
BssNI GRCGYC 1 cut(s) 1525
BssT1I CCWWGG 6 cut(s) 37, 72, 573, 1079, 2185, 2432
Bst2UI CCWGG 4 cut(s) 1110, 1346, 1430, 1785
Bst4CI ACNGT 7 cut(s) 237, 249, 1456, 1480, 1759, 2684, 2911
Bst6I CTCTTC 3 cut(s) 426, 1787, 2186
BstACI GRCGYC 1 cut(s) 1525
BstAPI GCANNNNNTGC 1 cut(s) 2213
BstC8I GCNNGC 3 cut(s) 1847, 2282, 3049
BstDEI CTNAG 5 cut(s) 392, 1039, 1199, 1415, 2660
BstDSI CCRYGG 2 cut(s) 72, 1079
BstEII GGTNACC 2 cut(s) 1084, 3100
BstF5I GGATG 3 cut(s) 2267, 2698, 2883
BstFNI CGCG 3 cut(s) 2851, 2868, 2985
BstH2I RGCGCY 2 cut(s) 260, 1528
BstHHI GCGC 3 cut(s) 259, 1099, 1527
BstMAI GTCTC 2 cut(s) 1407, 1994
BstMWI GCNNNNNNNGC 5 cut(s) 83, 92, 551, 2213, 2848
BstNI CCWGG 4 cut(s) 1110, 1346, 1430, 1785
BstNSI RCATGY 2 cut(s) 348, 745
BstPI GGTNACC 2 cut(s) 1084, 3100
BstSCI CCNGG 6 cut(s) 1108, 1344, 1405, 1428, 1783, 3028
BstSFI CTRYAG 1 cut(s) 2226
BstUI CGCG 3 cut(s) 2851, 2868, 2985
BstV1I GCAGC 2 cut(s) 1673, 3045
BstV2I GAAGAC 2 cut(s) 1064, 2674
BstX2I RGATCY 2 cut(s) 1552, 2764
BstYI RGATCY 2 cut(s) 1552, 2764
Bsu15I ATCGAT 1 cut(s) 3091
BsuRI GGCC 9 cut(s) 492, 565, 996, 1248, 1428, 1447, 1713, 2115, 2854
BsuTUI ATCGAT 1 cut(s) 3091
BtgI CCRYGG 2 cut(s) 72, 1079
BtgZI GCGATG 1 cut(s) 2222
BtsCI GGATG 3 cut(s) 2267, 2698, 2883
BtsI GCAGTG 3 cut(s) 292, 821, 2235
BtuMI TCGCGA 1 cut(s) 2985
BveI ACCTGC 4 cut(s) 383, 1580, 1691, 2358
Cac8I GCNNGC 3 cut(s) 1847, 2282, 3049
CaiI CAGNNNCTG 2 cut(s) 967, 1478
CciI TCATGA 1 cut(s) 2416
CfoI GCGC 3 cut(s) 259, 1099, 1527
ClaI ATCGAT 1 cut(s) 3091
CseI GACGC 2 cut(s) 538, 2857
Csp6I GTAC 8 cut(s) 354, 369, 1272, 1420, 1494, 2130, 2550, 2907
CspCI CAANNNNNGTGG 2 cut(s) 1168, 1203
CviQI GTAC 8 cut(s) 354, 369, 1272, 1420, 1494, 2130, 2550, 2907
DdeI CTNAG 5 cut(s) 392, 1039, 1199, 1415, 2660
DinI GGCGCC 1 cut(s) 1526
DrdI GACNNNNNNGTC 1 cut(s) 535
DseDI GACNNNNNNGTC 1 cut(s) 535
EaeI YGGCCR 1 cut(s) 2852
Eam1104I CTCTTC 3 cut(s) 426, 1787, 2186
EarI CTCTTC 3 cut(s) 426, 1787, 2186
Ecl136II GAGCTC 2 cut(s) 852, 3016
Eco130I CCWWGG 6 cut(s) 37, 72, 573, 1079, 2185, 2432
Eco24I GRGCYC 2 cut(s) 854, 3018
Eco32I GATATC 1 cut(s) 1537
Eco47I GGWCC 5 cut(s) 103, 595, 1680, 1956, 2837
Eco53kI GAGCTC 2 cut(s) 852, 3016
Eco57I CTGAAG 5 cut(s) 132, 476, 611, 1109, 3008
Eco88I CYCGRG 1 cut(s) 2553
Eco91I GGTNACC 2 cut(s) 1084, 3100
EcoICRI GAGCTC 2 cut(s) 852, 3016
EcoO109I RGGNCCY 4 cut(s) 491, 595, 1680, 1956
EcoO65I GGTNACC 2 cut(s) 1084, 3100
EcoRII CCWGG 4 cut(s) 1108, 1344, 1428, 1783
EcoRV GATATC 1 cut(s) 1537
EcoT14I CCWWGG 6 cut(s) 37, 72, 573, 1079, 2185, 2432
EcoT22I ATGCAT 3 cut(s) 556, 913, 1995
EcoT38I GRGCYC 2 cut(s) 854, 3018
EgeI GGCGCC 1 cut(s) 1526
EheI GGCGCC 1 cut(s) 1526
ErhI CCWWGG 6 cut(s) 37, 72, 573, 1079, 2185, 2432
FaqI GGGAC 6 cut(s) 1375, 1693, 1969, 2578, 2850, 2957
FauI CCCGC 2 cut(s) 508, 1291
FbaI TGATCA 2 cut(s) 2323, 2788
Fnu4HI GCNGC 3 cut(s) 1687, 2852, 3034
FokI GGATG 3 cut(s) 2274, 2705, 2890
FriOI GRGCYC 2 cut(s) 854, 3018
Fsp4HI GCNGC 3 cut(s) 1687, 2852, 3034
FspBI CTAG 9 cut(s) 38, 407, 1614, 2004, 2015, 2021, 2096, 2246, 3048
GlaI GCGC 3 cut(s) 258, 1098, 1526
GluI GCNGC 3 cut(s) 1687, 2852, 3034
GsaI CCCAGC 1 cut(s) 1253
GsuI CTGGAG 4 cut(s) 630, 656, 1457, 2907
HaeII RGCGCY 2 cut(s) 260, 1528
HaeIII GGCC 9 cut(s) 492, 565, 996, 1248, 1428, 1447, 1713, 2115, 2854
HapII CCGG 2 cut(s) 1407, 3030
HgaI GACGC 2 cut(s) 538, 2857
HhaI GCGC 3 cut(s) 259, 1099, 1527
Hin1I GRCGYC 1 cut(s) 1525
Hin6I GCGC 3 cut(s) 257, 1097, 1525
HinP1I GCGC 3 cut(s) 257, 1097, 1525
HinfI GANTC 8 cut(s) 828, 1069, 1321, 1768, 2194, 2423, 2656, 2728
HpaII CCGG 2 cut(s) 1407, 3030
HphI GGTGA 5 cut(s) 719, 1348, 1715, 2174, 3094
Hpy166II GTNNAC 5 cut(s) 698, 1190, 2122, 2132, 2603
Hpy8I GTNNAC 5 cut(s) 698, 1190, 2122, 2132, 2603
HpyCH4III ACNGT 7 cut(s) 237, 249, 1456, 1480, 1759, 2684, 2911
HpyCH4IV ACGT 1 cut(s) 66
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 92, 551, 2213, 2848
HpyF3I CTNAG 5 cut(s) 392, 1039, 1199, 1415, 2660
HpySE526I ACGT 1 cut(s) 66
Hsp92I GRCGYC 1 cut(s) 1525
HspAI GCGC 3 cut(s) 257, 1097, 1525
KasI GGCGCC 1 cut(s) 1524
Ksp22I TGATCA 2 cut(s) 2323, 2788
LguI GCTCTTC 2 cut(s) 426, 1787
LmnI GCTCC 4 cut(s) 242, 675, 849, 857
Lsp1109I GCAGC 2 cut(s) 1673, 3045
LweI GCATC 5 cut(s) 767, 2186, 2252, 2683, 2868
MaeI CTAG 9 cut(s) 38, 407, 1614, 2004, 2015, 2021, 2096, 2246, 3048
MaeII ACGT 1 cut(s) 66
MfeI CAATTG 1 cut(s) 540
MflI RGATCY 2 cut(s) 1552, 2764
MhlI GDGCHC 4 cut(s) 247, 323, 854, 3018
MluI ACGCGT 1 cut(s) 2866
Mly113I GGCGCC 1 cut(s) 1525
MlyI GAGTC 4 cut(s) 1078, 2203, 2432, 2722
MmeI TCCRAC 2 cut(s) 256, 1778
Mph1103I ATGCAT 3 cut(s) 556, 913, 1995
MroXI GAANNNNTTC 2 cut(s) 435, 482
MseI TTAA 8 cut(s) 174, 186, 1017, 1629, 1731, 1853, 2079, 3087
MslI CAYNNNNRTG 2 cut(s) 25, 2448
MspA1I CMGCKG 2 cut(s) 693, 2664
MspI CCGG 2 cut(s) 1407, 3030
MspR9I CCNGG 6 cut(s) 1110, 1346, 1407, 1430, 1785, 3030
MunI CAATTG 1 cut(s) 540
Mva1269I GAATGC 2 cut(s) 7, 554
MvaI CCWGG 4 cut(s) 1110, 1346, 1430, 1785
MvnI CGCG 3 cut(s) 2851, 2868, 2985
MwoI GCNNNNNNNGC 5 cut(s) 83, 92, 551, 2213, 2848
NarI GGCGCC 1 cut(s) 1525
NciI CCSGG 2 cut(s) 1407, 3030
NcoI CCATGG 2 cut(s) 72, 1079
NheI GCTAGC 1 cut(s) 3047
NlaIV GGNNCC 8 cut(s) 105, 493, 1526, 1681, 1957, 2583, 2766, 2838
NmuCI GTSAC 5 cut(s) 164, 1354, 1369, 2862, 3100
NruI TCGCGA 1 cut(s) 2985
NsiI ATGCAT 3 cut(s) 556, 913, 1995
NspI RCATGY 2 cut(s) 348, 745
PagI TCATGA 1 cut(s) 2416
PaqCI CACCTGC 2 cut(s) 383, 1580
PciI ACATGT 1 cut(s) 344
PciSI GCTCTTC 2 cut(s) 426, 1787
PctI GAATGC 2 cut(s) 7, 554
PdmI GAANNNNTTC 2 cut(s) 435, 482
PfeI GAWTC 4 cut(s) 828, 1321, 1768, 2656
PflMI CCANNNNNTGG 2 cut(s) 287, 653
PfoI TCCNGGA 2 cut(s) 1405, 1783
PkrI GCNGC 3 cut(s) 1688, 2853, 3035
PleI GAGTC 4 cut(s) 1077, 2202, 2431, 2722
PluTI GGCGCC 1 cut(s) 1528
PpsI GAGTC 4 cut(s) 1077, 2202, 2431, 2722
PpuMI RGGWCCY 3 cut(s) 595, 1680, 1956
PscI ACATGT 1 cut(s) 344
PsiI TTATAA 1 cut(s) 3113
Psp124BI GAGCTC 2 cut(s) 854, 3018
Psp5II RGGWCCY 3 cut(s) 595, 1680, 1956
Psp6I CCWGG 4 cut(s) 1108, 1344, 1428, 1783
PspEI GGTNACC 2 cut(s) 1084, 3100
PspFI CCCAGC 1 cut(s) 1249
PspGI CCWGG 4 cut(s) 1108, 1344, 1428, 1783
PspN4I GGNNCC 8 cut(s) 105, 493, 1526, 1681, 1957, 2583, 2766, 2838
PspPPI RGGWCCY 3 cut(s) 595, 1680, 1956
PstI CTGCAG 1 cut(s) 2230
PstNI CAGNNNCTG 2 cut(s) 967, 1478
PsuI RGATCY 2 cut(s) 1552, 2764
PvuII CAGCTG 1 cut(s) 693
RruI TCGCGA 1 cut(s) 2985
RsaI GTAC 8 cut(s) 355, 370, 1273, 1421, 1495, 2131, 2551, 2908
RsaNI GTAC 8 cut(s) 354, 369, 1272, 1420, 1494, 2130, 2550, 2907
RseI CAYNNNNRTG 2 cut(s) 25, 2448
SacI GAGCTC 2 cut(s) 854, 3018
SapI GCTCTTC 2 cut(s) 426, 1787
SaqAI TTAA 8 cut(s) 174, 186, 1017, 1629, 1731, 1853, 2079, 3087
SatI GCNGC 3 cut(s) 1687, 2852, 3034
ScaI AGTACT 1 cut(s) 2908
SchI GAGTC 4 cut(s) 1078, 2203, 2432, 2722
ScrFI CCNGG 6 cut(s) 1110, 1346, 1407, 1430, 1785, 3030
SduI GDGCHC 4 cut(s) 247, 323, 854, 3018
SfaNI GCATC 5 cut(s) 767, 2186, 2252, 2683, 2868
SfcI CTRYAG 1 cut(s) 2226
SfoI GGCGCC 1 cut(s) 1526
SinI GGWCC 5 cut(s) 103, 595, 1680, 1956, 2837
SmiMI CAYNNNNRTG 2 cut(s) 25, 2448
SmlI CTYRAG 1 cut(s) 1393
SmoI CTYRAG 1 cut(s) 1393
SsiI CCGC 6 cut(s) 501, 1286, 1298, 2664, 2840, 2851
SspDI GGCGCC 1 cut(s) 1524
SspI AATATT 2 cut(s) 1857, 2467
SspMI CTAG 9 cut(s) 38, 407, 1614, 2004, 2015, 2021, 2096, 2246, 3048
SstI GAGCTC 2 cut(s) 854, 3018
StyD4I CCNGG 6 cut(s) 1108, 1344, 1405, 1428, 1783, 3028
StyI CCWWGG 6 cut(s) 37, 72, 573, 1079, 2185, 2432
TaaI ACNGT 7 cut(s) 237, 249, 1456, 1480, 1759, 2684, 2911
TaiI ACGT 1 cut(s) 69
TaqI TCGA 5 cut(s) 179, 1580, 2271, 2475, 3091
TaqII GACCGA 1 cut(s) 2627
TatI WGTACW 2 cut(s) 1271, 2906
TauI GCSGC 1 cut(s) 2854
TfiI GAWTC 4 cut(s) 828, 1321, 1768, 2656
Tru1I TTAA 8 cut(s) 174, 186, 1017, 1629, 1731, 1853, 2079, 3087
Tru9I TTAA 8 cut(s) 174, 186, 1017, 1629, 1731, 1853, 2079, 3087
TseFI GTSAC 5 cut(s) 164, 1354, 1369, 2862, 3100
TseI GCWGC 2 cut(s) 1686, 3033
Tsp45I GTSAC 5 cut(s) 164, 1354, 1369, 2862, 3100
TspGWI ACGGA 1 cut(s) 116
Van91I CCANNNNNTGG 2 cut(s) 287, 653
VpaK11BI GGWCC 5 cut(s) 103, 595, 1680, 1956, 2837
XapI RAATTY 6 cut(s) 711, 874, 1132, 1168, 1745, 2286
XbaI TCTAGA 1 cut(s) 406
XceI RCATGY 2 cut(s) 348, 745
XcmI CCANNNNNNNNNTGG 1 cut(s) 1004
XmaJI CCTAGG 1 cut(s) 37
XmnI GAANNNNTTC 2 cut(s) 435, 482
XspI CTAG 9 cut(s) 38, 407, 1614, 2004, 2015, 2021, 2096, 2246, 3048
ZrmI AGTACT 1 cut(s) 2908
Zsp2I ATGCAT 3 cut(s) 556, 913, 1995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.