Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 221 |
| AccI | GTMKAC | 1 cut(s) | 1093 |
| AciI | CCGC | 2 cut(s) | 457, 1165 |
| AclWI | GGATC | 5 cut(s) | 53, 635, 692, 705, 879 |
| AcsI | RAATTY | 5 cut(s) | 718, 833, 938, 1070, 1359 |
| AcuI | CTGAAG | 3 cut(s) | 683, 1077, 1151 |
| AfaI | GTAC | 4 cut(s) | 97, 240, 329, 579 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 297, 485, 523, 941 |
| AflII | CTTAAG | 1 cut(s) | 506 |
| AflIII | ACRYGT | 1 cut(s) | 144 |
| AjnI | CCWGG | 1 cut(s) | 795 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 155, 187, 800, 832 |
| AluBI | AGCT | 3 cut(s) | 358, 730, 1367 |
| AluI | AGCT | 3 cut(s) | 358, 730, 1367 |
| Alw26I | GTCTC | 2 cut(s) | 107, 728 |
| AlwI | GGATC | 5 cut(s) | 53, 635, 692, 705, 879 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 987 |
| AoxI | GGCC | 1 cut(s) | 1237 |
| ApeKI | GCWGC | 1 cut(s) | 570 |
| ApoI | RAATTY | 5 cut(s) | 718, 833, 938, 1070, 1359 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 106, 138 |
| AseI | ATTAAT | 1 cut(s) | 690 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 560, 1151 |
| AspA2I | CCTAGG | 1 cut(s) | 1001 |
| AspLEI | GCGC | 1 cut(s) | 865 |
| AspS9I | GGNCC | 1 cut(s) | 1238 |
| AsuC2I | CCSGG | 1 cut(s) | 935 |
| AsuII | TTCGAA | 1 cut(s) | 1256 |
| AvrII | CCTAGG | 1 cut(s) | 1001 |
| AxyI | CCTNAGG | 1 cut(s) | 431 |
| BamHI | GGATCC | 1 cut(s) | 697 |
| BbsI | GAAGAC | 1 cut(s) | 1139 |
| BbvI | GCAGC | 1 cut(s) | 557 |
| BccI | CCATC | 4 cut(s) | 129, 593, 626, 1168 |
| BciT130I | CCWGG | 1 cut(s) | 797 |
| BcnI | CCSGG | 1 cut(s) | 935 |
| BcoDI | GTCTC | 2 cut(s) | 107, 728 |
| BfaI | CTAG | 3 cut(s) | 965, 1002, 1353 |
| BfmI | CTRYAG | 1 cut(s) | 333 |
| BfoI | RGCGCY | 1 cut(s) | 866 |
| BfrI | CTTAAG | 1 cut(s) | 506 |
| BglII | AGATCT | 2 cut(s) | 645, 1078 |
| BisI | GCNGC | 1 cut(s) | 571 |
| BlnI | CCTAGG | 1 cut(s) | 1001 |
| BlsI | GCNGC | 1 cut(s) | 572 |
| Bme1390I | CCNGG | 2 cut(s) | 797, 935 |
| BmgT120I | GGNCC | 1 cut(s) | 1238 |
| BmiI | GGNNCC | 2 cut(s) | 604, 699 |
| BmrFI | CCNGG | 2 cut(s) | 797, 935 |
| BmsI | GCATC | 5 cut(s) | 52, 854, 937, 974, 1324 |
| BpiI | GAAGAC | 1 cut(s) | 1139 |
| BpmI | CTGGAG | 2 cut(s) | 639, 1108 |
| Bpu14I | TTCGAA | 1 cut(s) | 1256 |
| BpuEI | CTTGAG | 2 cut(s) | 752, 995 |
| BpuMI | CCSGG | 1 cut(s) | 935 |
| Bsa29I | ATCGAT | 2 cut(s) | 630, 705 |
| BsaI | GGTCTC | 1 cut(s) | 728 |
| BsaJI | CCNNGG | 3 cut(s) | 795, 915, 1001 |
| BsaWI | WCCGGW | 1 cut(s) | 1096 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 297, 485, 523, 941 |
| Bse1I | ACTGG | 2 cut(s) | 388, 1091 |
| Bse21I | CCTNAGG | 1 cut(s) | 431 |
| Bse3DI | GCAATG | 1 cut(s) | 864 |
| BseBI | CCWGG | 1 cut(s) | 797 |
| BseCI | ATCGAT | 2 cut(s) | 630, 705 |
| BseDI | CCNNGG | 3 cut(s) | 795, 915, 1001 |
| BseGI | GGATG | 2 cut(s) | 330, 845 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 297, 485, 523, 941 |
| BseMI | GCAATG | 1 cut(s) | 864 |
| BseMII | CTCAG | 1 cut(s) | 396 |
| BseNI | ACTGG | 2 cut(s) | 388, 1091 |
| BseRI | GAGGAG | 1 cut(s) | 1280 |
| BseXI | GCAGC | 1 cut(s) | 557 |
| BseYI | CCCAGC | 1 cut(s) | 987 |
| BsgI | GTGCAG | 1 cut(s) | 1067 |
| BshFI | GGCC | 1 cut(s) | 1239 |
| BshVI | ATCGAT | 2 cut(s) | 630, 705 |
| BsiSI | CCGG | 2 cut(s) | 935, 1097 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 297, 485, 523, 941 |
| BsmAI | GTCTC | 2 cut(s) | 107, 728 |
| BsmI | GAATGC | 1 cut(s) | 1021 |
| BsnI | GGCC | 1 cut(s) | 1239 |
| Bso31I | GGTCTC | 1 cut(s) | 728 |
| Bsp119I | TTCGAA | 1 cut(s) | 1256 |
| Bsp1407I | TGTACA | 1 cut(s) | 327 |
| Bsp143I | GATC | 8 cut(s) | 45, 361, 627, 645, 697, 742, 871, 1078 |
| Bsp19I | CCATGG | 1 cut(s) | 915 |
| BspACI | CCGC | 2 cut(s) | 457, 1165 |
| BspANI | GGCC | 1 cut(s) | 1239 |
| BspCNI | CTCAG | 1 cut(s) | 397 |
| BspDI | ATCGAT | 2 cut(s) | 630, 705 |
| BspHI | TCATGA | 1 cut(s) | 540 |
| BspLI | GGNNCC | 2 cut(s) | 604, 699 |
| BspMAI | CTGCAG | 1 cut(s) | 337 |
| BspPI | GGATC | 5 cut(s) | 53, 635, 692, 705, 879 |
| BspT104I | TTCGAA | 1 cut(s) | 1256 |
| BspTI | CTTAAG | 1 cut(s) | 506 |
| BspTNI | GGTCTC | 1 cut(s) | 728 |
| BsrDI | GCAATG | 1 cut(s) | 864 |
| BsrGI | TGTACA | 1 cut(s) | 327 |
| BsrI | ACTGG | 2 cut(s) | 388, 1091 |
| BssECI | CCNNGG | 3 cut(s) | 795, 915, 1001 |
| BssMI | GATC | 8 cut(s) | 45, 361, 627, 645, 697, 742, 871, 1078 |
| BssT1I | CCWWGG | 2 cut(s) | 915, 1001 |
| Bst2UI | CCWGG | 1 cut(s) | 797 |
| Bst4CI | ACNGT | 4 cut(s) | 100, 526, 828, 854 |
| Bst6I | CTCTTC | 2 cut(s) | 190, 1126 |
| BstAFI | CTTAAG | 1 cut(s) | 506 |
| BstAUI | TGTACA | 1 cut(s) | 327 |
| BstBI | TTCGAA | 1 cut(s) | 1256 |
| BstDEI | CTNAG | 3 cut(s) | 405, 431, 1042 |
| BstDSI | CCRYGG | 1 cut(s) | 915 |
| BstF5I | GGATG | 2 cut(s) | 330, 845 |
| BstH2I | RGCGCY | 1 cut(s) | 866 |
| BstHHI | GCGC | 1 cut(s) | 865 |
| BstKTI | GATC | 8 cut(s) | 48, 364, 630, 648, 700, 745, 874, 1081 |
| BstMAI | GTCTC | 2 cut(s) | 107, 728 |
| BstMBI | GATC | 8 cut(s) | 45, 361, 627, 645, 697, 742, 871, 1078 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 812 |
| BstNI | CCWGG | 1 cut(s) | 797 |
| BstNSI | RCATGY | 2 cut(s) | 148, 250 |
| BstSCI | CCNGG | 2 cut(s) | 795, 933 |
| BstSFI | CTRYAG | 1 cut(s) | 333 |
| BstV1I | GCAGC | 1 cut(s) | 557 |
| BstV2I | GAAGAC | 1 cut(s) | 1139 |
| BstX2I | RGATCY | 3 cut(s) | 645, 697, 1078 |
| BstYI | RGATCY | 3 cut(s) | 645, 697, 1078 |
| Bsu15I | ATCGAT | 2 cut(s) | 630, 705 |
| Bsu36I | CCTNAGG | 1 cut(s) | 431 |
| BsuRI | GGCC | 1 cut(s) | 1239 |
| BsuTUI | ATCGAT | 2 cut(s) | 630, 705 |
| BtgI | CCRYGG | 1 cut(s) | 915 |
| BtsCI | GGATG | 2 cut(s) | 330, 845 |
| BtsI | GCAGTG | 1 cut(s) | 330 |
| BtsIMutI | CAGTG | 3 cut(s) | 55, 330, 1084 |
| CaiI | CAGNNNCTG | 1 cut(s) | 987 |
| CciI | TCATGA | 1 cut(s) | 540 |
| CfoI | GCGC | 1 cut(s) | 865 |
| Cfr13I | GGNCC | 1 cut(s) | 1238 |
| ClaI | ATCGAT | 2 cut(s) | 630, 705 |
| CseI | GACGC | 1 cut(s) | 970 |
| Csp6I | GTAC | 4 cut(s) | 96, 239, 328, 578 |
| CviAII | CATG | 5 cut(s) | 145, 247, 518, 541, 916 |
| CviJI | RGCY | 7 cut(s) | 152, 358, 489, 730, 908, 1239, 1367 |
| CviKI_1 | RGCY | 7 cut(s) | 152, 358, 489, 730, 908, 1239, 1367 |
| CviQI | GTAC | 4 cut(s) | 96, 239, 328, 578 |
| DdeI | CTNAG | 3 cut(s) | 405, 431, 1042 |
| DpnI | GATC | 8 cut(s) | 47, 363, 629, 647, 699, 744, 873, 1080 |
| DpnII | GATC | 8 cut(s) | 45, 361, 627, 645, 697, 742, 871, 1078 |
| Eam1104I | CTCTTC | 2 cut(s) | 190, 1126 |
| EarI | CTCTTC | 2 cut(s) | 190, 1126 |
| Eco130I | CCWWGG | 2 cut(s) | 915, 1001 |
| Eco31I | GGTCTC | 1 cut(s) | 728 |
| Eco57I | CTGAAG | 3 cut(s) | 683, 1077, 1151 |
| Eco81I | CCTNAGG | 1 cut(s) | 431 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1238 |
| EcoRI | GAATTC | 1 cut(s) | 1359 |
| EcoRII | CCWGG | 1 cut(s) | 795 |
| EcoT14I | CCWWGG | 2 cut(s) | 915, 1001 |
| ErhI | CCWWGG | 2 cut(s) | 915, 1001 |
| FaeI | CATG | 5 cut(s) | 148, 250, 521, 544, 919 |
| FatI | CATG | 5 cut(s) | 144, 246, 517, 540, 915 |
| FauI | CCCGC | 1 cut(s) | 1158 |
| FauNDI | CATATG | 1 cut(s) | 369 |
| FblI | GTMKAC | 1 cut(s) | 1093 |
| Fnu4HI | GCNGC | 1 cut(s) | 571 |
| FokI | GGATG | 2 cut(s) | 337, 832 |
| Fsp4HI | GCNGC | 1 cut(s) | 571 |
| FspBI | CTAG | 3 cut(s) | 965, 1002, 1353 |
| GlaI | GCGC | 1 cut(s) | 864 |
| GluI | GCNGC | 1 cut(s) | 571 |
| GsaI | CCCAGC | 1 cut(s) | 991 |
| GsuI | CTGGAG | 2 cut(s) | 639, 1108 |
| HaeII | RGCGCY | 1 cut(s) | 866 |
| HaeIII | GGCC | 1 cut(s) | 1239 |
| HapII | CCGG | 2 cut(s) | 935, 1097 |
| HgaI | GACGC | 1 cut(s) | 970 |
| HhaI | GCGC | 1 cut(s) | 865 |
| Hin1II | CATG | 5 cut(s) | 148, 250, 521, 544, 919 |
| Hin6I | GCGC | 1 cut(s) | 863 |
| HinP1I | GCGC | 1 cut(s) | 863 |
| HincII | GTYRAC | 1 cut(s) | 952 |
| HindII | GTYRAC | 1 cut(s) | 952 |
| HindIII | AAGCTT | 1 cut(s) | 728 |
| HinfI | GANTC | 6 cut(s) | 232, 254, 448, 556, 1090, 1152 |
| HpaII | CCGG | 2 cut(s) | 935, 1097 |
| Hpy166II | GTNNAC | 5 cut(s) | 239, 330, 712, 952, 1094 |
| Hpy188I | TCNGA | 4 cut(s) | 262, 821, 832, 1078 |
| Hpy188III | TCNNGA | 7 cut(s) | 541, 643, 656, 695, 869, 1012, 1028 |
| Hpy8I | GTNNAC | 5 cut(s) | 239, 330, 712, 952, 1094 |
| Hpy99I | CGWCG | 1 cut(s) | 104 |
| HpyAV | CCTTC | 3 cut(s) | 1052, 1141, 1155 |
| HpyCH4III | ACNGT | 4 cut(s) | 100, 526, 828, 854 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 812 |
| HpyF3I | CTNAG | 3 cut(s) | 405, 431, 1042 |
| Hsp92II | CATG | 5 cut(s) | 148, 250, 521, 544, 919 |
| HspAI | GCGC | 1 cut(s) | 863 |
| Kzo9I | GATC | 8 cut(s) | 45, 361, 627, 645, 697, 742, 871, 1078 |
| LmnI | GCTCC | 1 cut(s) | 658 |
| Lsp1109I | GCAGC | 1 cut(s) | 557 |
| LweI | GCATC | 5 cut(s) | 52, 854, 937, 974, 1324 |
| MaeI | CTAG | 3 cut(s) | 965, 1002, 1353 |
| MaeIII | GTNAC | 4 cut(s) | 74, 251, 520, 1231 |
| MalI | GATC | 8 cut(s) | 47, 363, 629, 647, 699, 744, 873, 1080 |
| MboI | GATC | 8 cut(s) | 45, 361, 627, 645, 697, 742, 871, 1078 |
| MboII | GAAGA | 9 cut(s) | 151, 207, 356, 596, 676, 1043, 1058, 1113, 1144 |
| MfeI | CAATTG | 1 cut(s) | 956 |
| MflI | RGATCY | 3 cut(s) | 645, 697, 1078 |
| MlyI | GAGTC | 3 cut(s) | 241, 248, 1099 |
| MmeI | TCCRAC | 1 cut(s) | 873 |
| MroXI | GAANNNNTTC | 2 cut(s) | 560, 1151 |
| MseI | TTAA | 3 cut(s) | 507, 690, 888 |
| MslI | CAYNNNNRTG | 1 cut(s) | 611 |
| MspCI | CTTAAG | 1 cut(s) | 506 |
| MspI | CCGG | 2 cut(s) | 935, 1097 |
| MspR9I | CCNGG | 2 cut(s) | 797, 935 |
| MunI | CAATTG | 1 cut(s) | 956 |
| Mva1269I | GAATGC | 1 cut(s) | 1021 |
| MvaI | CCWGG | 1 cut(s) | 797 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 812 |
| NciI | CCSGG | 1 cut(s) | 935 |
| NcoI | CCATGG | 1 cut(s) | 915 |
| NdeI | CATATG | 1 cut(s) | 369 |
| NdeII | GATC | 8 cut(s) | 45, 361, 627, 645, 697, 742, 871, 1078 |
| NlaIII | CATG | 5 cut(s) | 148, 250, 521, 544, 919 |
| NlaIV | GGNNCC | 2 cut(s) | 604, 699 |
| NmuCI | GTSAC | 2 cut(s) | 74, 251 |
| NspI | RCATGY | 2 cut(s) | 148, 250 |
| NspV | TTCGAA | 1 cut(s) | 1256 |
| PagI | TCATGA | 1 cut(s) | 540 |
| PciI | ACATGT | 1 cut(s) | 144 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 14 |
| PctI | GAATGC | 1 cut(s) | 1021 |
| PdmI | GAANNNNTTC | 2 cut(s) | 560, 1151 |
| PfeI | GAWTC | 3 cut(s) | 448, 556, 1152 |
| PfoI | TCCNGGA | 1 cut(s) | 933 |
| PkrI | GCNGC | 1 cut(s) | 572 |
| PleI | GAGTC | 3 cut(s) | 240, 248, 1098 |
| PpsI | GAGTC | 3 cut(s) | 240, 248, 1098 |
| PscI | ACATGT | 1 cut(s) | 144 |
| PshBI | ATTAAT | 1 cut(s) | 690 |
| PsiI | TTATAA | 1 cut(s) | 221 |
| Psp6I | CCWGG | 1 cut(s) | 795 |
| PspFI | CCCAGC | 1 cut(s) | 987 |
| PspGI | CCWGG | 1 cut(s) | 795 |
| PspN4I | GGNNCC | 2 cut(s) | 604, 699 |
| PspPI | GGNCC | 1 cut(s) | 1238 |
| PstI | CTGCAG | 1 cut(s) | 337 |
| PstNI | CAGNNNCTG | 1 cut(s) | 987 |
| PsuI | RGATCY | 3 cut(s) | 645, 697, 1078 |
| RsaI | GTAC | 4 cut(s) | 97, 240, 329, 579 |
| RsaNI | GTAC | 4 cut(s) | 96, 239, 328, 578 |
| RseI | CAYNNNNRTG | 1 cut(s) | 611 |
| SaqAI | TTAA | 3 cut(s) | 507, 690, 888 |
| SatI | GCNGC | 1 cut(s) | 571 |
| Sau3AI | GATC | 8 cut(s) | 45, 361, 627, 645, 697, 742, 871, 1078 |
| Sau96I | GGNCC | 1 cut(s) | 1238 |
| SchI | GAGTC | 3 cut(s) | 241, 248, 1099 |
| ScrFI | CCNGG | 2 cut(s) | 797, 935 |
| SfaNI | GCATC | 5 cut(s) | 52, 854, 937, 974, 1324 |
| SfcI | CTRYAG | 1 cut(s) | 333 |
| SfuI | TTCGAA | 1 cut(s) | 1256 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 611 |
| SmlI | CTYRAG | 3 cut(s) | 506, 731, 1010 |
| SmoI | CTYRAG | 3 cut(s) | 506, 731, 1010 |
| SsiI | CCGC | 2 cut(s) | 457, 1165 |
| SspI | AATATT | 1 cut(s) | 811 |
| SspMI | CTAG | 3 cut(s) | 965, 1002, 1353 |
| StyD4I | CCNGG | 2 cut(s) | 795, 933 |
| StyI | CCWWGG | 2 cut(s) | 915, 1001 |
| TaaI | ACNGT | 4 cut(s) | 100, 526, 828, 854 |
| TaqI | TCGA | 7 cut(s) | 102, 174, 630, 705, 1029, 1256, 1317 |
| TatI | WGTACW | 3 cut(s) | 238, 327, 577 |
| TfiI | GAWTC | 3 cut(s) | 448, 556, 1152 |
| Tru1I | TTAA | 3 cut(s) | 507, 690, 888 |
| Tru9I | TTAA | 3 cut(s) | 507, 690, 888 |
| TscAI | CASTG | 3 cut(s) | 55, 337, 1091 |
| TseFI | GTSAC | 2 cut(s) | 74, 251 |
| TseI | GCWGC | 1 cut(s) | 570 |
| Tsp45I | GTSAC | 2 cut(s) | 74, 251 |
| TspDTI | ATGAA | 2 cut(s) | 356, 1239 |
| TspGWI | ACGGA | 1 cut(s) | 145 |
| TspRI | CASTG | 3 cut(s) | 55, 337, 1091 |
| Vha464I | CTTAAG | 1 cut(s) | 506 |
| VspI | ATTAAT | 1 cut(s) | 690 |
| XapI | RAATTY | 5 cut(s) | 718, 833, 938, 1070, 1359 |
| XceI | RCATGY | 2 cut(s) | 148, 250 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 856 |
| XmaJI | CCTAGG | 1 cut(s) | 1001 |
| XmiI | GTMKAC | 1 cut(s) | 1093 |
| XmnI | GAANNNNTTC | 2 cut(s) | 560, 1151 |
| XspI | CTAG | 3 cut(s) | 965, 1002, 1353 |