Rw2G024860
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000088
Physical Location & Seq
Forward (+)
36985347 .. 36996279
10933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G024860.1

Sequence Viewer

Length: 3090 bp
ATGTTGTTTGGCGTGGATTGGTGGTCTTGGCGGGAGATATGGCCGGAGGAATTCCTTGTGTTTGGTGGTGCAGCGCGAGCTCTGAGTTCAATCTTCCAGCAATGGCAGATTGAGGCCAAATGGAACACAACTAGAGATCCATGCAGTGGAGCGGCCATTAAGGACTCCATCGAAATCGGCAACGAAGCTTACAACCCTTTTGCTAAATGCAATTGTTCAAACTCCGCTTGCCACATTACCCAACTGAAAGTTTCTACATTGGAGGTTGTTGGCATAATTCCGGAACAGCTATGGACTTTGAAGTTTCTCACTCATCTCGATTTAGGTCACAATCACTTGACAGGACCTCTCTCTGCATCAATTGGAAATCTGAGCAGCTTGCAATATTTGAGCTTAGGCCATAACATGTTATCAGGCCAGCTACCAAAGGAACTCGGAAATCTCTCTAATTTGACATTCTTGGCTATTGGGACTATGAACTTTTCCGGTGGTCTACCACATGAGCTAGGGAATTTATTAAATTTACAACAGATTTACTTGGATAGTTCTGGAGTTAGCGGTGAGATTCCACCGACGTTTGCTAATCTACGAAACTTGACCATATTCCGGGTGTCAGACACAGAACTCTCTGGCAGGATACCTGACTTCGTAGGATATTGGTCAAAGCTTACTTCCTTGGTATTTCAAGGGAACTCTTTTGAAGGTCCTATACCAGCTACACTGTCCAATCTGACTTCTCTTGAGGAGCTGAGAATTAGTGATATAATATCTACTGCCAGTGGGAACTCTTCTCTTGGATTTATTAAGGATATGAAGTCAAGATCTCTAAAAGTCCTAGTGCTGCGAAATAACAATATTTCTGATTCAATTCCTTCTAATATTGGAGAGTTGCAAAGTTTGTATCACCTGGATTTAAGCTTCAACAATTTAGCAGGAGTAATACCACCTTCCCTGTTTAATTTGCGTTCGCTCAAAGCCCTGTTTCTTGGAAACAACAAGTTGAGTGGTAACCTTCCTGAAAATAAAAGCTCGTCTCTTGTCAATGTAGATCTGTCTTACAATAATCTAGTAGGGTCGAGCTTTCCTTCTTGGGTGAACGAAGACTTACAGCTTAATTTAGTTGCCAACAACTTCAGCGCAGAAAATTCAAACAGCAGATCTTTGCCTTCAGGGTTGAAATGCCTCCAAAGGAACTTCCCTTGCAATGTTAACCCTGGAATCCAACGTTACATTGGGATTAAATGTGGTGGTCCGATCACGTCTTCCAATGGGATTGTGTATGATCAGGAAAATGAGACCCTTGGTCCAGCTACATATTTTGTGACTGGCACAAGCAGATGGGGAGTTAGCAATGTTGGTATTAAAACAGAGTATCAGGATGGATATATCACGGGAAACATTCCTGAGTATACAGTTTCCTCGTCATCTCAATTCACGAATACTTCAGATCCTGAGCTATTCCGGACTGCAAGGATCTCTGCTTCATCACTTAGATACTATGGCTTGGGGCTGGAGAATGGTAACTACACGGTCAAACTTCAGTTTGCAGAACAAAAAATCCCAGATACTCCAAAAAATCTTGGGAGACGTGTATTTGATATATATATCCAGGGAATTCTAGTTTTGAAGGATTTTGATATACGAAAGGAAGCAGGTGGGAAATCCTTGCAAGCCGTTCAGAAGGAATATAAGGCCCAGGTTTCACAGAACTACCTTGAAATTCATCTCTTTTGGGCTGGAAAGGGGACTTGCTGCGTACCTGAGGTTGGTACTTATGGACCTGTTATTTCAGCCATCAGTGCTACACCAGATTTCCCCCCTACTATCATTCCGAACCATCCAACTAGTAACAAGAATAGGACGGGGCTGATTGTGGGGATTGTTGTTGGTGGTGGAGTACTTTTGATTCTATTGTTGTTGGTTTTCTGTATAGTTCAAAGAAGAAAAAGGATTAACACCAGTAAAAATGAAGATCTGTTGGGAATAGATATTGGACCACTCACTTTCAGTTTTTCAGAGCTAAAGACAGCTACAGATGACTTCAGTCCAGCGAATAAGATTGGAGAGGGAGGATTTGGACCTGTCTATAAAGGTACTCTTAATGATGGAAGAGTAATTGCCGTGAAGCAATTGTCTGCAGCATCCCAGCAAGGAAAAAATGAGTTTGTAGCTGAAATTGCCACCATATCTGCCGTGCGACACAATAACCTAGTGAATTTGCATGGATTCTGCACTGAGGGAGTTAAACGGCTCCTTGTCTATGAGTATCTGGAAAACAAGAGTCTCGATCATGCATTATTTGGAAAAAGAAGTTTGAATCTTGATTGGTCATCAAGATTTGATATATGCTTGGGCGTGGCTAAAGGTTTGACTTATCTCCATGAGGAATCAAGGGTTCGGATTGTACACAGAGATGTGAAGGCCAGTAATATTCTGCTCGACTCTAATCTCATCCCCAAAATATCAGATTTCGGTTTGGCCAAGCTTTATGATGATAAAAAGACCCACATCAGTACTCGTGTTGCGGGAACAATAGGGTATCTTGCACCGGAATATGCCATGCGTGGACACTTGACACAGAAGACTGATGTGTTTGCCTTTGGTGTTCTTGCTCTAGAAATTGTCAGTGGTAGGCCAAATTCTGACCGGAGCTTGGATGGCGAGATGATTTATCTTCTCGAATGGGCTTGGCACTTGCATGAAAACAAACGCGAAGTTGAACTAGTGGACTCTAGGTTATCAGAATTCAATGAGGAAGAGGCAAGACGAATAACCAAGATAGGACTTTTGTGCACTCAAACATCACCAATGCAACGACCACCGATGTCGCGTGTGGTGGGAATGCTTTCAGGAGATATTGAAGTAATGACAGTAACTTCAAAGCCTGGTTACTTGACAGACTGGGAATTTGACGATACAAGTACTTTCTCCAGTCTAACCACTGAAATGTCAGCAAAGGGAAATGATCCTAGCTTTGACAACTCACCGGCGAGTACAAGCACTTTGGGGGATCCAGGGCAGCTTTCTACAAATGCAACGGATGCCTTGCTTCATAATATTGTTGTTGATACCCAATCCCTACCGACTTAA

Protein Analysis

1029

Amino Acids

112.97

Weight (kDa)

5.95

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 91 - 181 4.4e-08 Leucine-rich repeat region
LRR_14 PF23598 168 - 258 1.6e-07 Leucine-rich repeat region
LRR_14 PF23598 273 - 333 6.3e-06 Leucine-rich repeat region
LRR_8 PF13855 274 - 334 1.6e-07 Leucine rich repeat
Malectin PF11721 411 - 599 3.3e-42 Malectin domain
Pkinase PF00069 684 - 882 3e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 685 - 947 1.2e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1643
Acc36I ACCTGC 1 cut(s) 1643
AccB7I CCANNNNNTGG 1 cut(s) 146
AccBSI CCGCTC 1 cut(s) 152
AccI GTMKAC 2 cut(s) 493, 1409
AccII CGCG 3 cut(s) 76, 2712, 2830
AccIII TCCGGA 2 cut(s) 280, 1461
AciI CCGC 5 cut(s) 31, 152, 225, 558, 2525
AclI AACGTT 1 cut(s) 1225
AclWI GGATC 6 cut(s) 131, 1442, 1481, 2960, 3005, 3018
AcoI YGGCCR 3 cut(s) 41, 153, 2478
AcuI CTGAAG 5 cut(s) 1117, 1152, 1428, 1523, 2026
AfaI GTAC 8 cut(s) 1758, 1771, 1899, 2095, 2406, 2515, 2923, 2995
AfiI CCNNNNNNNGG 4 cut(s) 146, 606, 1766, 2653
AflIII ACRYGT 2 cut(s) 405, 1588
AhdI GACNNNNNGTC 1 cut(s) 1302
AhlI ACTAGT 2 cut(s) 1844, 2722
AjiI CACGTC 2 cut(s) 1260, 1589
AjnI CCWGG 6 cut(s) 906, 1213, 1608, 1695, 2884, 3013
AloI GAACNNNNNNTCC 2 cut(s) 1542, 1574
Alw21I GWGCWC 2 cut(s) 82, 2795
Alw26I GTCTC 4 cut(s) 1038, 1289, 1579, 2288
Alw44I GTGCAC 1 cut(s) 2791
AlwI GGATC 6 cut(s) 131, 1442, 1481, 2960, 3005, 3018
AlwNI CAGNNNCTG 1 cut(s) 1451
Aor13HI TCCGGA 2 cut(s) 280, 1461
AoxI GGCC 9 cut(s) 41, 114, 153, 397, 415, 1692, 2421, 2478, 2633
ApaLI GTGCAC 1 cut(s) 2791
ApeKI GCWGC 6 cut(s) 71, 375, 841, 1752, 2138, 3019
ArsI GACNNNNNNTTYG 2 cut(s) 2891, 2923
Asp700I GAANNNNTTC 1 cut(s) 2845
AspLEI GCGC 2 cut(s) 76, 1139
AspS9I GGNCC 8 cut(s) 344, 704, 1250, 1304, 1693, 1778, 1994, 2078
AsuC2I CCSGG 1 cut(s) 608
AsuHPI GGTGA 5 cut(s) 572, 896, 1105, 2796, 2976
AvaII GGWCC 7 cut(s) 344, 704, 1250, 1304, 1778, 1994, 2078
AxyI CCTNAGG 1 cut(s) 1761
BaeGI GKGCMC 1 cut(s) 2795
BalI TGGCCA 1 cut(s) 2480
BamHI GGATCC 1 cut(s) 3010
BanII GRGCYC 1 cut(s) 82
BarI GAAGNNNNNNTAC 2 cut(s) 693, 725
BauI CACGAG 1 cut(s) 2517
BbsI GAAGAC 3 cut(s) 1107, 1254, 2588
Bbv12I GWGCWC 2 cut(s) 82, 2795
BbvI GCAGC 6 cut(s) 83, 387, 828, 1739, 2150, 3031
BccI CCATC 7 cut(s) 176, 1332, 1373, 1802, 1845, 2099, 2651
BceAI ACGGC 4 cut(s) 1658, 2105, 2177, 2264
BciT130I CCWGG 6 cut(s) 908, 1215, 1610, 1697, 2886, 3015
BciVI GTATCC 1 cut(s) 630
BclI TGATCA 1 cut(s) 1282
BcnI CCSGG 1 cut(s) 608
BcoDI GTCTC 4 cut(s) 1038, 1289, 1579, 2288
BcuI ACTAGT 2 cut(s) 1844, 2722
BfmI CTRYAG 2 cut(s) 2031, 2136
BfuAI ACCTGC 1 cut(s) 1643
BfuI GTATCC 1 cut(s) 630
BglII AGATCT 4 cut(s) 821, 1048, 1157, 1972
BisI GCNGC 7 cut(s) 72, 153, 376, 842, 1753, 2139, 3020
BlsI GCNGC 7 cut(s) 73, 154, 377, 843, 1754, 2140, 3021
BmcAI AGTACT 3 cut(s) 1899, 2515, 2923
Bme1390I CCNGG 7 cut(s) 608, 908, 1215, 1610, 1697, 2886, 3015
Bme18I GGWCC 7 cut(s) 344, 704, 1250, 1304, 1778, 1994, 2078
BmeRI GACNNNNNGTC 1 cut(s) 1302
BmgBI CACGTC 2 cut(s) 1260, 1589
BmgT120I GGNCC 8 cut(s) 344, 704, 1250, 1304, 1693, 1778, 1994, 2078
BmiI GGNNCC 2 cut(s) 2252, 3012
BmrFI CCNGG 7 cut(s) 608, 908, 1215, 1610, 1697, 2886, 3015
BmrI ACTGGG 1 cut(s) 2911
BmsI GCATC 3 cut(s) 365, 2150, 3031
BmuI ACTGGG 1 cut(s) 2911
BoxI GACNNNNGTC 1 cut(s) 2043
BpiI GAAGAC 3 cut(s) 1107, 1254, 2588
BpmI CTGGAG 3 cut(s) 570, 1532, 2914
Bpu10I CCTNAGC 2 cut(s) 394, 1452
BpuEI CTTGAG 1 cut(s) 761
BpuMI CCSGG 1 cut(s) 608
BsaI GGTCTC 1 cut(s) 1289
BsaJI CCNNGG 6 cut(s) 675, 1213, 1300, 1609, 1695, 3014
BsaWI WCCGGW 5 cut(s) 280, 485, 1461, 2548, 2646
Bsc4I CCNNNNNNNGG 4 cut(s) 146, 606, 1766, 2653
Bse118I RCCGGY 1 cut(s) 2986
Bse1I ACTGG 6 cut(s) 777, 1330, 1959, 2424, 2906, 2931
Bse21I CCTNAGG 1 cut(s) 1761
Bse3DI GCAATG 3 cut(s) 107, 1210, 1357
BseAI TCCGGA 2 cut(s) 280, 1461
BseBI CCWGG 6 cut(s) 908, 1215, 1610, 1697, 2886, 3015
BseDI CCNNGG 6 cut(s) 675, 1213, 1300, 1609, 1695, 3014
BseGI GGATG 6 cut(s) 1384, 1837, 2141, 2451, 2662, 3046
BseLI CCNNNNNNNGG 4 cut(s) 146, 606, 1766, 2653
BseMI GCAATG 3 cut(s) 107, 1210, 1357
BseMII CTCAG 7 cut(s) 74, 362, 740, 1395, 1443, 1752, 2226
BseNI ACTGG 6 cut(s) 777, 1330, 1959, 2424, 2906, 2931
BseRI GAGGAG 1 cut(s) 758
BseSI GKGCMC 1 cut(s) 2795
BseXI GCAGC 6 cut(s) 83, 387, 828, 1739, 2150, 3031
BseYI CCCAGC 1 cut(s) 2145
BsgI GTGCAG 2 cut(s) 90, 2215
Bsh1236I CGCG 3 cut(s) 76, 2712, 2830
BshFI GGCC 9 cut(s) 43, 116, 155, 399, 417, 1694, 2423, 2480, 2635
BsiHKAI GWGCWC 2 cut(s) 82, 2795
BsiSI CCGG 8 cut(s) 44, 281, 486, 607, 1462, 2549, 2647, 2987
BslFI GGGAC 2 cut(s) 484, 1759
BslI CCNNNNNNNGG 4 cut(s) 146, 606, 1766, 2653
BsmAI GTCTC 4 cut(s) 1038, 1289, 1579, 2288
BsmBI CGTCTC 2 cut(s) 1038, 1579
BsmFI GGGAC 2 cut(s) 484, 1759
BsmI GAATGC 1 cut(s) 2847
BsnI GGCC 9 cut(s) 43, 116, 155, 399, 417, 1694, 2423, 2480, 2635
Bso31I GGTCTC 1 cut(s) 1289
Bsp1286I GDGCHC 2 cut(s) 82, 2795
Bsp13I TCCGGA 2 cut(s) 280, 1461
Bsp1407I TGTACA 1 cut(s) 2404
BspACI CCGC 5 cut(s) 31, 152, 225, 558, 2525
BspANI GGCC 9 cut(s) 43, 116, 155, 399, 417, 1694, 2423, 2480, 2635
BspCNI CTCAG 7 cut(s) 75, 363, 741, 1396, 1444, 1753, 2227
BspEI TCCGGA 2 cut(s) 280, 1461
BspFNI CGCG 3 cut(s) 76, 2712, 2830
BspLI GGNNCC 2 cut(s) 2252, 3012
BspMAI CTGCAG 1 cut(s) 2140
BspMI ACCTGC 1 cut(s) 1643
BspPI GGATC 6 cut(s) 131, 1442, 1481, 2960, 3005, 3018
BspTNI GGTCTC 1 cut(s) 1289
BsrBI CCGCTC 1 cut(s) 152
BsrDI GCAATG 3 cut(s) 107, 1210, 1357
BsrFI RCCGGY 1 cut(s) 2986
BsrGI TGTACA 1 cut(s) 2404
BsrI ACTGG 6 cut(s) 777, 1330, 1959, 2424, 2906, 2931
BssAI RCCGGY 1 cut(s) 2986
BssECI CCNNGG 6 cut(s) 675, 1213, 1300, 1609, 1695, 3014
BssNAI GTATAC 1 cut(s) 1410
BssSI CACGAG 1 cut(s) 2517
BssT1I CCWWGG 2 cut(s) 675, 1300
Bst1107I GTATAC 1 cut(s) 1410
Bst2BI CACGAG 1 cut(s) 2517
Bst2UI CCWGG 6 cut(s) 908, 1215, 1610, 1697, 2886, 3015
Bst4CI ACNGT 4 cut(s) 723, 1414, 1531, 2872
Bst6I CTCTTC 3 cut(s) 793, 2104, 2751
BstAPI GCANNNNNTGC 1 cut(s) 3041
BstAUI TGTACA 1 cut(s) 2404
BstC8I GCNNGC 5 cut(s) 78, 229, 380, 419, 1671
BstDEI CTNAG 9 cut(s) 83, 371, 394, 749, 1404, 1452, 1490, 1761, 2235
BstEII GGTNACC 1 cut(s) 1007
BstF5I GGATG 6 cut(s) 1384, 1837, 2141, 2451, 2662, 3046
BstFNI CGCG 3 cut(s) 76, 2712, 2830
BstHHI GCGC 2 cut(s) 76, 1139
BstMAI GTCTC 4 cut(s) 1038, 1289, 1579, 2288
BstMWI GCNNNNNNNGC 5 cut(s) 77, 1799, 2177, 2658, 3041
BstNI CCWGG 6 cut(s) 908, 1215, 1610, 1697, 2886, 3015
BstNSI RCATGY 1 cut(s) 409
BstPAI GACNNNNGTC 1 cut(s) 2043
BstPI GGTNACC 1 cut(s) 1007
BstSCI CCNGG 7 cut(s) 606, 906, 1213, 1608, 1695, 2884, 3013
BstSFI CTRYAG 2 cut(s) 2031, 2136
BstSLI GKGCMC 1 cut(s) 2795
BstUI CGCG 3 cut(s) 76, 2712, 2830
BstV1I GCAGC 6 cut(s) 83, 387, 828, 1739, 2150, 3031
BstV2I GAAGAC 3 cut(s) 1107, 1254, 2588
BstX2I RGATCY 8 cut(s) 136, 821, 1048, 1157, 1447, 1473, 1972, 3010
BstYI RGATCY 8 cut(s) 136, 821, 1048, 1157, 1447, 1473, 1972, 3010
BstZ17I GTATAC 1 cut(s) 1410
Bsu36I CCTNAGG 1 cut(s) 1761
BsuI GTATCC 1 cut(s) 630
BsuRI GGCC 9 cut(s) 43, 116, 155, 399, 417, 1694, 2423, 2480, 2635
BtrI CACGTC 2 cut(s) 1260, 1589
BtsCI GGATG 6 cut(s) 1384, 1837, 2141, 2451, 2662, 3046
BtsI GCAGTG 1 cut(s) 151
BtsIMutI CAGTG 7 cut(s) 151, 719, 784, 1804, 2232, 2632, 2940
BveI ACCTGC 1 cut(s) 1643
Cac8I GCNNGC 5 cut(s) 78, 229, 380, 419, 1671
CaiI CAGNNNCTG 1 cut(s) 1451
CfoI GCGC 2 cut(s) 76, 1139
Cfr10I RCCGGY 1 cut(s) 2986
Cfr13I GGNCC 8 cut(s) 344, 704, 1250, 1304, 1693, 1778, 1994, 2078
Csp6I GTAC 8 cut(s) 1757, 1770, 1898, 2094, 2405, 2514, 2922, 2994
CspCI CAANNNNNGTGG 2 cut(s) 985, 1020
CviAII CATG 8 cut(s) 141, 406, 500, 2222, 2291, 2381, 2560, 2699
CviQI GTAC 8 cut(s) 1757, 1770, 1898, 2094, 2405, 2514, 2922, 2994
DdeI CTNAG 9 cut(s) 83, 371, 394, 749, 1404, 1452, 1490, 1761, 2235
DriI GACNNNNNGTC 1 cut(s) 1302
EaeI YGGCCR 3 cut(s) 41, 153, 2478
Eam1104I CTCTTC 3 cut(s) 793, 2104, 2751
Eam1105I GACNNNNNGTC 1 cut(s) 1302
EarI CTCTTC 3 cut(s) 793, 2104, 2751
Ecl136II GAGCTC 1 cut(s) 80
Eco130I CCWWGG 2 cut(s) 675, 1300
Eco24I GRGCYC 1 cut(s) 82
Eco31I GGTCTC 1 cut(s) 1289
Eco47I GGWCC 7 cut(s) 344, 704, 1250, 1304, 1778, 1994, 2078
Eco53kI GAGCTC 1 cut(s) 80
Eco57I CTGAAG 5 cut(s) 1117, 1152, 1428, 1523, 2026
Eco81I CCTNAGG 1 cut(s) 1761
Eco91I GGTNACC 1 cut(s) 1007
EcoICRI GAGCTC 1 cut(s) 80
EcoO109I RGGNCCY 2 cut(s) 344, 704
EcoO65I GGTNACC 1 cut(s) 1007
EcoRI GAATTC 3 cut(s) 50, 1614, 2744
EcoRII CCWGG 6 cut(s) 906, 1213, 1608, 1695, 2884, 3013
EcoT14I CCWWGG 2 cut(s) 675, 1300
EcoT22I ATGCAT 1 cut(s) 2296
EcoT38I GRGCYC 1 cut(s) 82
ErhI CCWWGG 2 cut(s) 675, 1300
Esp3I CGTCTC 2 cut(s) 1038, 1579
FaeI CATG 8 cut(s) 144, 409, 503, 2225, 2294, 2384, 2563, 2702
FalI AAGNNNNNCTT 2 cut(s) 2082, 2114
FaqI GGGAC 2 cut(s) 484, 1759
FatI CATG 8 cut(s) 140, 405, 499, 2221, 2290, 2380, 2559, 2698
FauI CCCGC 2 cut(s) 24, 2518
FbaI TGATCA 1 cut(s) 1282
FblI GTMKAC 2 cut(s) 493, 1409
Fnu4HI GCNGC 7 cut(s) 72, 153, 376, 842, 1753, 2139, 3020
FokI GGATG 6 cut(s) 1391, 1824, 2128, 2438, 2669, 3053
FriOI GRGCYC 1 cut(s) 82
Fsp4HI GCNGC 7 cut(s) 72, 153, 376, 842, 1753, 2139, 3020
GlaI GCGC 2 cut(s) 75, 1138
GluI GCNGC 7 cut(s) 72, 153, 376, 842, 1753, 2139, 3020
GsaI CCCAGC 1 cut(s) 2149
GsuI CTGGAG 3 cut(s) 570, 1532, 2914
HaeIII GGCC 9 cut(s) 43, 116, 155, 399, 417, 1694, 2423, 2480, 2635
HapII CCGG 8 cut(s) 44, 281, 486, 607, 1462, 2549, 2647, 2987
HhaI GCGC 2 cut(s) 76, 1139
Hin1II CATG 8 cut(s) 144, 409, 503, 2225, 2294, 2384, 2563, 2702
Hin6I GCGC 2 cut(s) 74, 1137
HinP1I GCGC 2 cut(s) 74, 1137
HincII GTYRAC 1 cut(s) 1210
HindII GTYRAC 1 cut(s) 1210
HindIII AAGCTT 4 cut(s) 186, 665, 916, 2483
HpaI GTTAAC 1 cut(s) 1210
HpaII CCGG 8 cut(s) 44, 281, 486, 607, 1462, 2549, 2647, 2987
HphI GGTGA 5 cut(s) 572, 896, 1105, 2796, 2976
Hpy166II GTNNAC 8 cut(s) 494, 1096, 1210, 1410, 2407, 2567, 2728, 2793
Hpy8I GTNNAC 8 cut(s) 494, 1096, 1210, 1410, 2407, 2567, 2728, 2793
Hpy99I CGWCG 1 cut(s) 577
HpyAV CCTTC 9 cut(s) 695, 882, 957, 1022, 1095, 1176, 1621, 1675, 2413
HpyCH4III ACNGT 4 cut(s) 723, 1414, 1531, 2872
HpyCH4IV ACGT 4 cut(s) 575, 1225, 1259, 1588
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 1799, 2177, 2658, 3041
HpyF3I CTNAG 9 cut(s) 83, 371, 394, 749, 1404, 1452, 1490, 1761, 2235
HpySE526I ACGT 4 cut(s) 575, 1225, 1259, 1588
Hsp92II CATG 8 cut(s) 144, 409, 503, 2225, 2294, 2384, 2563, 2702
HspAI GCGC 2 cut(s) 74, 1137
Kpn2I TCCGGA 2 cut(s) 280, 1461
Ksp22I TGATCA 1 cut(s) 1282
KspAI GTTAAC 1 cut(s) 1210
LmnI GCTCC 4 cut(s) 149, 745, 2256, 2649
Lsp1109I GCAGC 6 cut(s) 83, 387, 828, 1739, 2150, 3031
LweI GCATC 3 cut(s) 365, 2150, 3031
MaeII ACGT 4 cut(s) 575, 1225, 1259, 1588
MaeIII GTNAC 8 cut(s) 326, 1007, 1226, 1321, 1520, 1847, 2872, 2888
MbiI CCGCTC 1 cut(s) 152
MfeI CAATTG 3 cut(s) 211, 360, 2129
MflI RGATCY 8 cut(s) 136, 821, 1048, 1157, 1447, 1473, 1972, 3010
MhlI GDGCHC 2 cut(s) 82, 2795
MlsI TGGCCA 1 cut(s) 2480
MluNI TGGCCA 1 cut(s) 2480
MlyI GAGTC 4 cut(s) 158, 2290, 2435, 2723
MmeI TCCRAC 2 cut(s) 1246, 1865
Mox20I TGGCCA 1 cut(s) 2480
Mph1103I ATGCAT 1 cut(s) 2296
MroI TCCGGA 2 cut(s) 280, 1461
MroXI GAANNNNTTC 1 cut(s) 2845
MscI TGGCCA 1 cut(s) 2480
MslI CAYNNNNRTG 3 cut(s) 2412, 2697, 2945
Msp20I TGGCCA 1 cut(s) 2480
MspI CCGG 8 cut(s) 44, 281, 486, 607, 1462, 2549, 2647, 2987
MspR9I CCNGG 7 cut(s) 608, 908, 1215, 1610, 1697, 2886, 3015
MunI CAATTG 3 cut(s) 211, 360, 2129
Mva1269I GAATGC 1 cut(s) 2847
MvaI CCWGG 6 cut(s) 908, 1215, 1610, 1697, 2886, 3015
MvnI CGCG 3 cut(s) 76, 2712, 2830
MwoI GCNNNNNNNGC 5 cut(s) 77, 1799, 2177, 2658, 3041
NciI CCSGG 1 cut(s) 608
NlaIII CATG 8 cut(s) 144, 409, 503, 2225, 2294, 2384, 2563, 2702
NlaIV GGNNCC 2 cut(s) 2252, 3012
NmuCI GTSAC 2 cut(s) 326, 1321
NsiI ATGCAT 1 cut(s) 2296
NspI RCATGY 1 cut(s) 409
PaqCI CACCTGC 1 cut(s) 1643
PciI ACATGT 1 cut(s) 405
PctI GAATGC 1 cut(s) 2847
PdmI GAANNNNTTC 1 cut(s) 2845
PfeI GAWTC 7 cut(s) 565, 863, 1218, 1906, 2226, 2317, 2387
PflMI CCANNNNNTGG 1 cut(s) 146
PkrI GCNGC 7 cut(s) 73, 154, 377, 843, 1754, 2140, 3021
PleI GAGTC 4 cut(s) 158, 2289, 2435, 2723
PpsI GAGTC 4 cut(s) 158, 2289, 2435, 2723
PpuMI RGGWCCY 2 cut(s) 344, 704
PscI ACATGT 1 cut(s) 405
PshAI GACNNNNGTC 1 cut(s) 2043
Psp124BI GAGCTC 1 cut(s) 82
Psp1406I AACGTT 1 cut(s) 1225
Psp5II RGGWCCY 2 cut(s) 344, 704
Psp6I CCWGG 6 cut(s) 906, 1213, 1608, 1695, 2884, 3013
PspEI GGTNACC 1 cut(s) 1007
PspFI CCCAGC 1 cut(s) 2145
PspGI CCWGG 6 cut(s) 906, 1213, 1608, 1695, 2884, 3013
PspN4I GGNNCC 2 cut(s) 2252, 3012
PspPI GGNCC 8 cut(s) 344, 704, 1250, 1304, 1693, 1778, 1994, 2078
PspPPI RGGWCCY 2 cut(s) 344, 704
PstI CTGCAG 1 cut(s) 2140
PstNI CAGNNNCTG 1 cut(s) 1451
PsuI RGATCY 8 cut(s) 136, 821, 1048, 1157, 1447, 1473, 1972, 3010
RsaI GTAC 8 cut(s) 1758, 1771, 1899, 2095, 2406, 2515, 2923, 2995
RsaNI GTAC 8 cut(s) 1757, 1770, 1898, 2094, 2405, 2514, 2922, 2994
RseI CAYNNNNRTG 3 cut(s) 2412, 2697, 2945
SacI GAGCTC 1 cut(s) 82
SatI GCNGC 7 cut(s) 72, 153, 376, 842, 1753, 2139, 3020
Sau96I GGNCC 8 cut(s) 344, 704, 1250, 1304, 1693, 1778, 1994, 2078
ScaI AGTACT 3 cut(s) 1899, 2515, 2923
SchI GAGTC 4 cut(s) 158, 2290, 2435, 2723
ScrFI CCNGG 7 cut(s) 608, 908, 1215, 1610, 1697, 2886, 3015
SduI GDGCHC 2 cut(s) 82, 2795
SfaNI GCATC 3 cut(s) 365, 2150, 3031
SfcI CTRYAG 2 cut(s) 2031, 2136
SgrAI CRCCGGYG 1 cut(s) 2986
SinI GGWCC 7 cut(s) 344, 704, 1250, 1304, 1778, 1994, 2078
SmiMI CAYNNNNRTG 3 cut(s) 2412, 2697, 2945
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
SpeI ACTAGT 2 cut(s) 1844, 2722
SsiI CCGC 5 cut(s) 31, 152, 225, 558, 2525
SspI AATATT 5 cut(s) 386, 856, 880, 2431, 3058
SstI GAGCTC 1 cut(s) 82
StyD4I CCNGG 7 cut(s) 606, 906, 1213, 1608, 1695, 2884, 3013
StyI CCWWGG 2 cut(s) 675, 1300
TaaI ACNGT 4 cut(s) 723, 1414, 1531, 2872
TaiI ACGT 4 cut(s) 578, 1228, 1262, 1591
TaqI TCGA 6 cut(s) 171, 318, 1076, 2286, 2439, 2679
TatI WGTACW 5 cut(s) 1897, 2404, 2513, 2921, 2993
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 7 cut(s) 565, 863, 1218, 1906, 2226, 2317, 2387
TscAI CASTG 7 cut(s) 151, 726, 784, 1804, 2239, 2632, 2947
TseFI GTSAC 2 cut(s) 326, 1321
TseI GCWGC 6 cut(s) 71, 375, 841, 1752, 2138, 3019
Tsp45I GTSAC 2 cut(s) 326, 1321
TspDTI ATGAA 7 cut(s) 491, 827, 1473, 1712, 1983, 2715, 3041
TspGWI ACGGA 1 cut(s) 3053
TspRI CASTG 7 cut(s) 151, 726, 784, 1804, 2239, 2632, 2947
Van91I CCANNNNNTGG 1 cut(s) 146
VneI GTGCAC 1 cut(s) 2791
VpaK11BI GGWCC 7 cut(s) 344, 704, 1250, 1304, 1778, 1994, 2078
XbaI TCTAGA 1 cut(s) 2614
XceI RCATGY 1 cut(s) 409
XcmI CCANNNNNNNNNTGG 1 cut(s) 1229
XmiI GTMKAC 2 cut(s) 493, 1409
XmnI GAANNNNTTC 1 cut(s) 2845
ZrmI AGTACT 3 cut(s) 1899, 2515, 2923
Zsp2I ATGCAT 1 cut(s) 2296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.