Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 593, 1334 |
| Acc36I | ACCTGC | 1 cut(s) | 1180 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 254 |
| AccI | GTMKAC | 1 cut(s) | 827 |
| AccII | CGCG | 1 cut(s) | 660 |
| AciI | CCGC | 2 cut(s) | 660, 1794 |
| AclWI | GGATC | 1 cut(s) | 134 |
| AcoI | YGGCCR | 1 cut(s) | 1732 |
| AcsI | RAATTY | 6 cut(s) | 211, 403, 515, 583, 798, 1910 |
| AcuI | CTGAAG | 3 cut(s) | 603, 914, 1275 |
| AfaI | GTAC | 6 cut(s) | 680, 859, 1149, 1162, 1572, 1681 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 254, 1157 |
| AflIII | ACRYGT | 1 cut(s) | 982 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 741 |
| AhlI | ACTAGT | 1 cut(s) | 1888 |
| AjiI | CACGTC | 1 cut(s) | 983 |
| AjnI | CCWGG | 5 cut(s) | 664, 1011, 1156, 1683, 2050 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 930, 962 |
| Alw21I | GWGCWC | 1 cut(s) | 1961 |
| Alw26I | GTCTC | 5 cut(s) | 25, 728, 973, 1998, 2013 |
| Alw44I | GTGCAC | 1 cut(s) | 1957 |
| AlwI | GGATC | 1 cut(s) | 134 |
| Ama87I | CYCGRG | 1 cut(s) | 326 |
| AoxI | GGCC | 6 cut(s) | 236, 661, 1191, 1587, 1732, 1857 |
| ApaLI | GTGCAC | 1 cut(s) | 1957 |
| ApeKI | GCWGC | 2 cut(s) | 1304, 2126 |
| ApoI | RAATTY | 6 cut(s) | 211, 403, 515, 583, 798, 1910 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2057, 2089 |
| AseI | ATTAAT | 1 cut(s) | 294 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2011 |
| AspLEI | GCGC | 1 cut(s) | 455 |
| AspS9I | GGNCC | 8 cut(s) | 236, 380, 662, 743, 1169, 1191, 1494, 1857 |
| AsuHPI | GGTGA | 1 cut(s) | 1962 |
| AsuII | TTCGAA | 1 cut(s) | 1564 |
| AvaI | CYCGRG | 1 cut(s) | 326 |
| AvaII | GGWCC | 4 cut(s) | 380, 743, 1169, 1494 |
| BaeGI | GKGCMC | 1 cut(s) | 1961 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1663, 1696 |
| BalI | TGGCCA | 1 cut(s) | 1734 |
| BauI | CACGAG | 2 cut(s) | 1813, 1995 |
| BbsI | GAAGAC | 5 cut(s) | 497, 626, 1754, 1804, 1833 |
| Bbv12I | GWGCWC | 1 cut(s) | 1961 |
| BbvI | GCAGC | 2 cut(s) | 1316, 2113 |
| BceAI | ACGGC | 1 cut(s) | 1271 |
| BciT130I | CCWGG | 5 cut(s) | 666, 1013, 1158, 1685, 2052 |
| BciVI | GTATCC | 2 cut(s) | 522, 1019 |
| BclI | TGATCA | 1 cut(s) | 1453 |
| BcoDI | GTCTC | 5 cut(s) | 25, 728, 973, 1998, 2013 |
| BcuI | ACTAGT | 1 cut(s) | 1888 |
| BfmI | CTRYAG | 2 cut(s) | 1302, 1356 |
| BfuAI | ACCTGC | 1 cut(s) | 1180 |
| BfuI | GTATCC | 2 cut(s) | 522, 1019 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1190 |
| BglII | AGATCT | 1 cut(s) | 698 |
| BisI | GCNGC | 3 cut(s) | 661, 1305, 2127 |
| BlsI | GCNGC | 3 cut(s) | 662, 1306, 2128 |
| BmcAI | AGTACT | 1 cut(s) | 859 |
| Bme1390I | CCNGG | 5 cut(s) | 666, 1013, 1158, 1685, 2052 |
| Bme18I | GGWCC | 4 cut(s) | 380, 743, 1169, 1494 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 741 |
| BmeT110I | CYCGRG | 1 cut(s) | 326 |
| BmgBI | CACGTC | 1 cut(s) | 983 |
| BmgT120I | GGNCC | 8 cut(s) | 236, 380, 662, 743, 1169, 1191, 1494, 1857 |
| BmiI | GGNNCC | 1 cut(s) | 382 |
| BmrFI | CCNGG | 5 cut(s) | 666, 1013, 1158, 1685, 2052 |
| BmrI | ACTGGG | 1 cut(s) | 1347 |
| BmsI | GCATC | 4 cut(s) | 512, 1061, 1316, 2071 |
| BmuI | ACTGGG | 1 cut(s) | 1347 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1754 |
| BpiI | GAAGAC | 5 cut(s) | 497, 626, 1754, 1804, 1833 |
| BpmI | CTGGAG | 1 cut(s) | 462 |
| Bpu14I | TTCGAA | 1 cut(s) | 1564 |
| BpuEI | CTTGAG | 4 cut(s) | 66, 90, 111, 301 |
| Bsa29I | ATCGAT | 1 cut(s) | 1208 |
| BsaI | GGTCTC | 1 cut(s) | 728 |
| BsaJI | CCNNGG | 6 cut(s) | 175, 286, 607, 664, 739, 1683 |
| BsaWI | WCCGGW | 1 cut(s) | 1714 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 254, 1157 |
| Bse1I | ACTGG | 7 cut(s) | 526, 799, 1199, 1327, 1342, 1590, 2072 |
| BseBI | CCWGG | 5 cut(s) | 666, 1013, 1158, 1685, 2052 |
| BseCI | ATCGAT | 1 cut(s) | 1208 |
| BseDI | CCNNGG | 6 cut(s) | 175, 286, 607, 664, 739, 1683 |
| BseGI | GGATG | 2 cut(s) | 1307, 1617 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 254, 1157 |
| BseMII | CTCAG | 2 cut(s) | 384, 1392 |
| BseNI | ACTGG | 7 cut(s) | 526, 799, 1199, 1327, 1342, 1590, 2072 |
| BseRI | GAGGAG | 1 cut(s) | 829 |
| BseSI | GKGCMC | 1 cut(s) | 1961 |
| BseXI | GCAGC | 2 cut(s) | 1316, 2113 |
| BseYI | CCCAGC | 1 cut(s) | 1691 |
| BsgI | GTGCAG | 1 cut(s) | 1381 |
| Bsh1236I | CGCG | 1 cut(s) | 660 |
| BshFI | GGCC | 6 cut(s) | 238, 663, 1193, 1589, 1734, 1859 |
| BshVI | ATCGAT | 1 cut(s) | 1208 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1961 |
| BsiHKCI | CYCGRG | 1 cut(s) | 326 |
| BsiSI | CCGG | 1 cut(s) | 1715 |
| BslFI | GGGAC | 4 cut(s) | 366, 376, 1150, 1708 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 254, 1157 |
| BsmAI | GTCTC | 5 cut(s) | 25, 728, 973, 1998, 2013 |
| BsmBI | CGTCTC | 2 cut(s) | 25, 973 |
| BsmFI | GGGAC | 4 cut(s) | 366, 376, 1150, 1708 |
| BsmI | GAATGC | 2 cut(s) | 654, 2013 |
| BsnI | GGCC | 6 cut(s) | 238, 663, 1193, 1589, 1734, 1859 |
| Bso31I | GGTCTC | 1 cut(s) | 728 |
| BsoBI | CYCGRG | 1 cut(s) | 326 |
| Bsp119I | TTCGAA | 1 cut(s) | 1564 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1961 |
| Bsp1407I | TGTACA | 1 cut(s) | 1570 |
| Bsp143I | GATC | 3 cut(s) | 139, 698, 1453 |
| Bsp19I | CCATGG | 1 cut(s) | 607 |
| BspACI | CCGC | 2 cut(s) | 660, 1794 |
| BspANI | GGCC | 6 cut(s) | 238, 663, 1193, 1589, 1734, 1859 |
| BspCNI | CTCAG | 2 cut(s) | 385, 1393 |
| BspDI | ATCGAT | 1 cut(s) | 1208 |
| BspFNI | CGCG | 1 cut(s) | 660 |
| BspHI | TCATGA | 1 cut(s) | 1546 |
| BspLI | GGNNCC | 1 cut(s) | 382 |
| BspMAI | CTGCAG | 2 cut(s) | 1306, 1360 |
| BspMI | ACCTGC | 1 cut(s) | 1180 |
| BspPI | GGATC | 1 cut(s) | 134 |
| BspT104I | TTCGAA | 1 cut(s) | 1564 |
| BspTNI | GGTCTC | 1 cut(s) | 728 |
| BsrGI | TGTACA | 1 cut(s) | 1570 |
| BsrI | ACTGG | 7 cut(s) | 526, 799, 1199, 1327, 1342, 1590, 2072 |
| BssECI | CCNNGG | 6 cut(s) | 175, 286, 607, 664, 739, 1683 |
| BssMI | GATC | 3 cut(s) | 139, 698, 1453 |
| BssNAI | GTATAC | 1 cut(s) | 828 |
| BssSI | CACGAG | 2 cut(s) | 1813, 1995 |
| BssT1I | CCWWGG | 4 cut(s) | 175, 286, 607, 739 |
| Bst1107I | GTATAC | 1 cut(s) | 828 |
| Bst2BI | CACGAG | 2 cut(s) | 1813, 1995 |
| Bst2UI | CCWGG | 5 cut(s) | 666, 1013, 1158, 1685, 2052 |
| Bst4CI | ACNGT | 5 cut(s) | 496, 683, 862, 1503, 2107 |
| Bst6I | CTCTTC | 2 cut(s) | 1071, 1270 |
| BstAUI | TGTACA | 1 cut(s) | 1570 |
| BstBI | TTCGAA | 1 cut(s) | 1564 |
| BstC8I | GCNNGC | 1 cut(s) | 2112 |
| BstDEI | CTNAG | 5 cut(s) | 393, 908, 1265, 1401, 1819 |
| BstDSI | CCRYGG | 1 cut(s) | 607 |
| BstF5I | GGATG | 2 cut(s) | 1307, 1617 |
| BstFNI | CGCG | 1 cut(s) | 660 |
| BstHHI | GCGC | 1 cut(s) | 455 |
| BstKTI | GATC | 3 cut(s) | 142, 701, 1456 |
| BstMAI | GTCTC | 5 cut(s) | 25, 728, 973, 1998, 2013 |
| BstMBI | GATC | 3 cut(s) | 139, 698, 1453 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 394, 660, 1181, 1190 |
| BstNI | CCWGG | 5 cut(s) | 666, 1013, 1158, 1685, 2052 |
| BstNSI | RCATGY | 1 cut(s) | 274 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1754 |
| BstSCI | CCNGG | 5 cut(s) | 664, 1011, 1156, 1683, 2050 |
| BstSFI | CTRYAG | 2 cut(s) | 1302, 1356 |
| BstSLI | GKGCMC | 1 cut(s) | 1961 |
| BstUI | CGCG | 1 cut(s) | 660 |
| BstV1I | GCAGC | 2 cut(s) | 1316, 2113 |
| BstV2I | GAAGAC | 5 cut(s) | 497, 626, 1754, 1804, 1833 |
| BstX2I | RGATCY | 1 cut(s) | 698 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1691 |
| BstYI | RGATCY | 1 cut(s) | 698 |
| BstZ17I | GTATAC | 1 cut(s) | 828 |
| Bsu15I | ATCGAT | 1 cut(s) | 1208 |
| BsuI | GTATCC | 2 cut(s) | 522, 1019 |
| BsuRI | GGCC | 6 cut(s) | 238, 663, 1193, 1589, 1734, 1859 |
| BsuTUI | ATCGAT | 1 cut(s) | 1208 |
| BtgI | CCRYGG | 1 cut(s) | 607 |
| BtrI | CACGTC | 1 cut(s) | 983 |
| BtsCI | GGATG | 2 cut(s) | 1307, 1617 |
| BtsI | GCAGTG | 1 cut(s) | 1365 |
| BtsIMutI | CAGTG | 6 cut(s) | 135, 336, 519, 792, 1365, 1398 |
| BveI | ACCTGC | 1 cut(s) | 1180 |
| Cac8I | GCNNGC | 1 cut(s) | 2112 |
| CciI | TCATGA | 1 cut(s) | 1546 |
| CfoI | GCGC | 1 cut(s) | 455 |
| Cfr13I | GGNCC | 8 cut(s) | 236, 380, 662, 743, 1169, 1191, 1494, 1857 |
| ClaI | ATCGAT | 1 cut(s) | 1208 |
| Csp6I | GTAC | 6 cut(s) | 679, 858, 1148, 1161, 1571, 1680 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1486, 1521 |
| CviAII | CATG | 8 cut(s) | 271, 608, 1088, 1516, 1547, 1675, 1726, 1865 |
| CviQI | GTAC | 6 cut(s) | 679, 858, 1148, 1161, 1571, 1680 |
| DdeI | CTNAG | 5 cut(s) | 393, 908, 1265, 1401, 1819 |
| DpnI | GATC | 3 cut(s) | 141, 700, 1455 |
| DpnII | GATC | 3 cut(s) | 139, 698, 1453 |
| DraI | TTTAAA | 1 cut(s) | 2119 |
| DriI | GACNNNNNGTC | 1 cut(s) | 741 |
| EaeI | YGGCCR | 1 cut(s) | 1732 |
| Eam1104I | CTCTTC | 2 cut(s) | 1071, 1270 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 741 |
| EarI | CTCTTC | 2 cut(s) | 1071, 1270 |
| Eco130I | CCWWGG | 4 cut(s) | 175, 286, 607, 739 |
| Eco31I | GGTCTC | 1 cut(s) | 728 |
| Eco47I | GGWCC | 4 cut(s) | 380, 743, 1169, 1494 |
| Eco57I | CTGAAG | 3 cut(s) | 603, 914, 1275 |
| Eco88I | CYCGRG | 1 cut(s) | 326 |
| EcoO109I | RGGNCCY | 1 cut(s) | 236 |
| EcoRI | GAATTC | 1 cut(s) | 515 |
| EcoRII | CCWGG | 5 cut(s) | 664, 1011, 1156, 1683, 2050 |
| EcoT14I | CCWWGG | 4 cut(s) | 175, 286, 607, 739 |
| EcoT22I | ATGCAT | 1 cut(s) | 1517 |
| ErhI | CCWWGG | 4 cut(s) | 175, 286, 607, 739 |
| Esp3I | CGTCTC | 2 cut(s) | 25, 973 |
| FaeI | CATG | 8 cut(s) | 274, 611, 1091, 1519, 1550, 1678, 1729, 1868 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1248, 1280 |
| FaqI | GGGAC | 4 cut(s) | 366, 376, 1150, 1708 |
| FatI | CATG | 8 cut(s) | 270, 607, 1087, 1515, 1546, 1674, 1725, 1864 |
| FbaI | TGATCA | 1 cut(s) | 1453 |
| FblI | GTMKAC | 1 cut(s) | 827 |
| Fnu4HI | GCNGC | 3 cut(s) | 661, 1305, 2127 |
| FokI | GGATG | 2 cut(s) | 1294, 1604 |
| Fsp4HI | GCNGC | 3 cut(s) | 661, 1305, 2127 |
| GlaI | GCGC | 1 cut(s) | 454 |
| GluI | GCNGC | 3 cut(s) | 661, 1305, 2127 |
| GsaI | CCCAGC | 1 cut(s) | 1695 |
| GsuI | CTGGAG | 1 cut(s) | 462 |
| HaeIII | GGCC | 6 cut(s) | 238, 663, 1193, 1589, 1734, 1859 |
| HapII | CCGG | 1 cut(s) | 1715 |
| HhaI | GCGC | 1 cut(s) | 455 |
| Hin1II | CATG | 8 cut(s) | 274, 611, 1091, 1519, 1550, 1678, 1729, 1868 |
| Hin6I | GCGC | 1 cut(s) | 453 |
| HinP1I | GCGC | 1 cut(s) | 453 |
| HindIII | AAGCTT | 2 cut(s) | 484, 1649 |
| HpaII | CCGG | 1 cut(s) | 1715 |
| HphI | GGTGA | 1 cut(s) | 1962 |
| Hpy166II | GTNNAC | 8 cut(s) | 604, 828, 1148, 1441, 1497, 1573, 1894, 1959 |
| Hpy188III | TCNNGA | 9 cut(s) | 6, 328, 441, 1451, 1547, 1781, 1844, 1899, 2016 |
| Hpy8I | GTNNAC | 8 cut(s) | 604, 828, 1148, 1441, 1497, 1573, 1894, 1959 |
| Hpy99I | CGWCG | 1 cut(s) | 20 |
| HpyCH4III | ACNGT | 5 cut(s) | 496, 683, 862, 1503, 2107 |
| HpyCH4IV | ACGT | 2 cut(s) | 982, 1876 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 394, 660, 1181, 1190 |
| HpyF3I | CTNAG | 5 cut(s) | 393, 908, 1265, 1401, 1819 |
| HpySE526I | ACGT | 2 cut(s) | 982, 1876 |
| Hsp92II | CATG | 8 cut(s) | 274, 611, 1091, 1519, 1550, 1678, 1729, 1868 |
| HspAI | GCGC | 1 cut(s) | 453 |
| Ksp22I | TGATCA | 1 cut(s) | 1453 |
| Kzo9I | GATC | 3 cut(s) | 139, 698, 1453 |
| LmnI | GCTCC | 4 cut(s) | 17, 91, 115, 810 |
| Lsp1109I | GCAGC | 2 cut(s) | 1316, 2113 |
| LweI | GCATC | 4 cut(s) | 512, 1061, 1316, 2071 |
| MaeII | ACGT | 2 cut(s) | 982, 1876 |
| MaeIII | GTNAC | 4 cut(s) | 903, 1378, 1808, 2029 |
| MalI | GATC | 3 cut(s) | 141, 700, 1455 |
| MboI | GATC | 3 cut(s) | 139, 698, 1453 |
| MfeI | CAATTG | 3 cut(s) | 252, 1295, 1804 |
| MflI | RGATCY | 1 cut(s) | 698 |
| MhlI | GDGCHC | 1 cut(s) | 1961 |
| MlsI | TGGCCA | 1 cut(s) | 1734 |
| MluNI | TGGCCA | 1 cut(s) | 1734 |
| MlyI | GAGTC | 4 cut(s) | 885, 1410, 1456, 1889 |
| MmeI | TCCRAC | 1 cut(s) | 1254 |
| Mox20I | TGGCCA | 1 cut(s) | 1734 |
| Mph1103I | ATGCAT | 1 cut(s) | 1517 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2011 |
| MscI | TGGCCA | 1 cut(s) | 1734 |
| MseI | TTAA | 6 cut(s) | 294, 344, 834, 2039, 2118, 2145 |
| MslI | CAYNNNNRTG | 3 cut(s) | 31, 1578, 2027 |
| Msp20I | TGGCCA | 1 cut(s) | 1734 |
| MspA1I | CMGCKG | 1 cut(s) | 1794 |
| MspI | CCGG | 1 cut(s) | 1715 |
| MspR9I | CCNGG | 5 cut(s) | 666, 1013, 1158, 1685, 2052 |
| MunI | CAATTG | 3 cut(s) | 252, 1295, 1804 |
| Mva1269I | GAATGC | 2 cut(s) | 654, 2013 |
| MvaI | CCWGG | 5 cut(s) | 666, 1013, 1158, 1685, 2052 |
| MvnI | CGCG | 1 cut(s) | 660 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 394, 660, 1181, 1190 |
| NcoI | CCATGG | 1 cut(s) | 607 |
| NdeII | GATC | 3 cut(s) | 139, 698, 1453 |
| NlaIII | CATG | 8 cut(s) | 274, 611, 1091, 1519, 1550, 1678, 1729, 1868 |
| NlaIV | GGNNCC | 1 cut(s) | 382 |
| NmuCI | GTSAC | 4 cut(s) | 903, 1378, 1808, 2029 |
| NsiI | ATGCAT | 1 cut(s) | 1517 |
| NspI | RCATGY | 1 cut(s) | 274 |
| NspV | TTCGAA | 1 cut(s) | 1564 |
| PagI | TCATGA | 1 cut(s) | 1546 |
| PctI | GAATGC | 2 cut(s) | 654, 2013 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2011 |
| PfeI | GAWTC | 9 cut(s) | 669, 866, 953, 1210, 1392, 1483, 1553, 1607, 1847 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 254 |
| PkrI | GCNGC | 3 cut(s) | 662, 1306, 2128 |
| PleI | GAGTC | 4 cut(s) | 885, 1410, 1455, 1889 |
| PpsI | GAGTC | 4 cut(s) | 885, 1410, 1455, 1889 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1754 |
| PshBI | ATTAAT | 1 cut(s) | 294 |
| PsiI | TTATAA | 2 cut(s) | 593, 1334 |
| Psp6I | CCWGG | 5 cut(s) | 664, 1011, 1156, 1683, 2050 |
| PspFI | CCCAGC | 1 cut(s) | 1691 |
| PspGI | CCWGG | 5 cut(s) | 664, 1011, 1156, 1683, 2050 |
| PspN4I | GGNNCC | 1 cut(s) | 382 |
| PspPI | GGNCC | 8 cut(s) | 236, 380, 662, 743, 1169, 1191, 1494, 1857 |
| PstI | CTGCAG | 2 cut(s) | 1306, 1360 |
| PsuI | RGATCY | 1 cut(s) | 698 |
| RsaI | GTAC | 6 cut(s) | 680, 859, 1149, 1162, 1572, 1681 |
| RsaNI | GTAC | 6 cut(s) | 679, 858, 1148, 1161, 1571, 1680 |
| RseI | CAYNNNNRTG | 3 cut(s) | 31, 1578, 2027 |
| SaqAI | TTAA | 6 cut(s) | 294, 344, 834, 2039, 2118, 2145 |
| SatI | GCNGC | 3 cut(s) | 661, 1305, 2127 |
| Sau3AI | GATC | 3 cut(s) | 139, 698, 1453 |
| Sau96I | GGNCC | 8 cut(s) | 236, 380, 662, 743, 1169, 1191, 1494, 1857 |
| ScaI | AGTACT | 1 cut(s) | 859 |
| SchI | GAGTC | 4 cut(s) | 885, 1410, 1456, 1889 |
| ScrFI | CCNGG | 5 cut(s) | 666, 1013, 1158, 1685, 2052 |
| SduI | GDGCHC | 1 cut(s) | 1961 |
| SfaNI | GCATC | 4 cut(s) | 512, 1061, 1316, 2071 |
| SfcI | CTRYAG | 2 cut(s) | 1302, 1356 |
| SfuI | TTCGAA | 1 cut(s) | 1564 |
| SinI | GGWCC | 4 cut(s) | 380, 743, 1169, 1494 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 31, 1578, 2027 |
| SmlI | CTYRAG | 4 cut(s) | 81, 105, 126, 280 |
| SmoI | CTYRAG | 4 cut(s) | 81, 105, 126, 280 |
| SpeI | ACTAGT | 1 cut(s) | 1888 |
| SsiI | CCGC | 2 cut(s) | 660, 1794 |
| SspI | AATATT | 1 cut(s) | 1597 |
| StyD4I | CCNGG | 5 cut(s) | 664, 1011, 1156, 1683, 2050 |
| StyI | CCWWGG | 4 cut(s) | 175, 286, 607, 739 |
| TaaI | ACNGT | 5 cut(s) | 496, 683, 862, 1503, 2107 |
| TaiI | ACGT | 2 cut(s) | 985, 1879 |
| TaqI | TCGA | 3 cut(s) | 1208, 1564, 1605 |
| TatI | WGTACW | 2 cut(s) | 857, 1570 |
| TauI | GCSGC | 1 cut(s) | 663 |
| TfiI | GAWTC | 9 cut(s) | 669, 866, 953, 1210, 1392, 1483, 1553, 1607, 1847 |
| Tru1I | TTAA | 6 cut(s) | 294, 344, 834, 2039, 2118, 2145 |
| Tru9I | TTAA | 6 cut(s) | 294, 344, 834, 2039, 2118, 2145 |
| TscAI | CASTG | 6 cut(s) | 142, 343, 526, 799, 1365, 1405 |
| TseFI | GTSAC | 4 cut(s) | 903, 1378, 1808, 2029 |
| TseI | GCWGC | 2 cut(s) | 1304, 2126 |
| Tsp45I | GTSAC | 4 cut(s) | 903, 1378, 1808, 2029 |
| TspDTI | ATGAA | 9 cut(s) | 40, 288, 528, 529, 951, 1535, 1839, 1881, 2154 |
| TspGWI | ACGGA | 1 cut(s) | 4 |
| TspRI | CASTG | 6 cut(s) | 142, 343, 526, 799, 1365, 1405 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 254 |
| VneI | GTGCAC | 1 cut(s) | 1957 |
| VpaK11BI | GGWCC | 4 cut(s) | 380, 743, 1169, 1494 |
| VspI | ATTAAT | 1 cut(s) | 294 |
| XapI | RAATTY | 6 cut(s) | 211, 403, 515, 583, 798, 1910 |
| XbaI | TCTAGA | 1 cut(s) | 1780 |
| XceI | RCATGY | 1 cut(s) | 274 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1334 |
| XmiI | GTMKAC | 1 cut(s) | 827 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2011 |
| ZrmI | AGTACT | 1 cut(s) | 859 |
| Zsp2I | ATGCAT | 1 cut(s) | 1517 |