Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 1057 |
| Acc36I | ACCTGC | 2 cut(s) | 1109, 1255 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 230 |
| AccBSI | CCGCTC | 1 cut(s) | 1231 |
| AccI | GTMKAC | 4 cut(s) | 21, 717, 869, 1222 |
| AccII | CGCG | 1 cut(s) | 1289 |
| AciI | CCGC | 4 cut(s) | 50, 79, 1231, 1748 |
| AclI | AACGTT | 1 cut(s) | 456 |
| AclWI | GGATC | 1 cut(s) | 1862 |
| AcsI | RAATTY | 8 cut(s) | 160, 349, 557, 1040, 1096, 1861, 1967, 2129 |
| AcuI | CTGAAG | 1 cut(s) | 47 |
| AcyI | GRCGYC | 1 cut(s) | 1054 |
| AdeI | CACNNNGTG | 2 cut(s) | 12, 1325 |
| AfaI | GTAC | 7 cut(s) | 44, 856, 1237, 1445, 1629, 1738, 2251 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 118, 230, 750, 1232, 1747, 1877, 2059 |
| AflIII | ACRYGT | 2 cut(s) | 1012, 1556 |
| AgeI | ACCGGT | 1 cut(s) | 1273 |
| AhlI | ACTAGT | 1 cut(s) | 1945 |
| AjnI | CCWGG | 3 cut(s) | 15, 1074, 1431 |
| AleI | CACNNNNGTG | 1 cut(s) | 2192 |
| Alw21I | GWGCWC | 2 cut(s) | 623, 2018 |
| Alw26I | GTCTC | 2 cut(s) | 213, 1045 |
| Alw44I | GTGCAC | 1 cut(s) | 2014 |
| AlwI | GGATC | 1 cut(s) | 1862 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1306 |
| AoxI | GGCC | 3 cut(s) | 189, 1644, 1856 |
| ApaLI | GTGCAC | 1 cut(s) | 2014 |
| ApeKI | GCWGC | 5 cut(s) | 146, 236, 1025, 1256, 1361 |
| ApoI | RAATTY | 8 cut(s) | 160, 349, 557, 1040, 1096, 1861, 1967, 2129 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2114, 2146 |
| AsiGI | ACCGGT | 1 cut(s) | 1273 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2068 |
| AspS9I | GGNCC | 5 cut(s) | 47, 189, 542, 785, 1244 |
| AsuC2I | CCSGG | 2 cut(s) | 1036, 2109 |
| AsuHPI | GGTGA | 4 cut(s) | 1892, 2019, 2143, 2179 |
| AsuII | TTCGAA | 2 cut(s) | 537, 1621 |
| AvaII | GGWCC | 4 cut(s) | 47, 542, 785, 1244 |
| BaeGI | GKGCMC | 1 cut(s) | 2018 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1720, 1753 |
| BanII | GRGCYC | 2 cut(s) | 623, 780 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 531, 563 |
| BauI | CACGAG | 1 cut(s) | 10 |
| BbsI | GAAGAC | 3 cut(s) | 30, 732, 1811 |
| Bbv12I | GWGCWC | 2 cut(s) | 623, 2018 |
| BbvCI | CCTCAGC | 1 cut(s) | 671 |
| BbvI | GCAGC | 5 cut(s) | 158, 223, 1012, 1268, 1373 |
| BccI | CCATC | 8 cut(s) | 120, 343, 743, 813, 1197, 1268, 1322, 2050 |
| BceAI | ACGGC | 1 cut(s) | 1173 |
| BciT130I | CCWGG | 3 cut(s) | 17, 1076, 1433 |
| BciVI | GTATCC | 1 cut(s) | 468 |
| BclI | TGATCA | 1 cut(s) | 1510 |
| BcnI | CCSGG | 2 cut(s) | 1036, 2109 |
| BcoDI | GTCTC | 2 cut(s) | 213, 1045 |
| BcuI | ACTAGT | 1 cut(s) | 1945 |
| BfaI | CTAG | 5 cut(s) | 344, 450, 1584, 1946, 2211 |
| BfmI | CTRYAG | 3 cut(s) | 1298, 1359, 2145 |
| BfuAI | ACCTGC | 2 cut(s) | 1109, 1255 |
| BfuI | GTATCC | 1 cut(s) | 468 |
| BisI | GCNGC | 5 cut(s) | 147, 237, 1026, 1257, 1362 |
| BlpI | GCTNAGC | 1 cut(s) | 1704 |
| BlsI | GCNGC | 5 cut(s) | 148, 238, 1027, 1258, 1363 |
| Bme1390I | CCNGG | 5 cut(s) | 17, 1036, 1076, 1433, 2109 |
| Bme18I | GGWCC | 4 cut(s) | 47, 542, 785, 1244 |
| BmgT120I | GGNCC | 5 cut(s) | 47, 189, 542, 785, 1244 |
| BmiI | GGNNCC | 1 cut(s) | 1475 |
| BmrFI | CCNGG | 5 cut(s) | 17, 1036, 1076, 1433, 2109 |
| BmsI | GCATC | 1 cut(s) | 1373 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1811 |
| BpiI | GAAGAC | 3 cut(s) | 30, 732, 1811 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 610, 642 |
| BpmI | CTGGAG | 2 cut(s) | 1514, 1939 |
| Bpu10I | CCTNAGC | 2 cut(s) | 671, 1227 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1704 |
| Bpu14I | TTCGAA | 2 cut(s) | 537, 1621 |
| BpuEI | CTTGAG | 6 cut(s) | 44, 71, 101, 535, 983, 992 |
| BpuMI | CCSGG | 2 cut(s) | 1036, 2109 |
| BsaHI | GRCGYC | 1 cut(s) | 1054 |
| BsaI | GGTCTC | 1 cut(s) | 213 |
| BsaJI | CCNNGG | 3 cut(s) | 781, 1075, 1372 |
| BsaWI | WCCGGW | 3 cut(s) | 215, 1273, 1771 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 118, 230, 750, 1232, 1747, 1877, 2059 |
| Bse118I | RCCGGY | 1 cut(s) | 1273 |
| Bse1I | ACTGG | 6 cut(s) | 811, 1311, 1384, 1647, 2129, 2172 |
| Bse3DI | GCAATG | 2 cut(s) | 209, 838 |
| BseBI | CCWGG | 3 cut(s) | 17, 1076, 1433 |
| BseDI | CCNNGG | 3 cut(s) | 781, 1075, 1372 |
| BseGI | GGATG | 3 cut(s) | 1364, 1674, 2042 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 118, 230, 750, 1232, 1747, 1877, 2059 |
| BseMI | GCAATG | 2 cut(s) | 209, 838 |
| BseMII | CTCAG | 8 cut(s) | 281, 330, 662, 720, 878, 1218, 1386, 1449 |
| BseNI | ACTGG | 6 cut(s) | 811, 1311, 1384, 1647, 2129, 2172 |
| BseSI | GKGCMC | 1 cut(s) | 2018 |
| BseXI | GCAGC | 5 cut(s) | 158, 223, 1012, 1268, 1373 |
| BsgI | GTGCAG | 1 cut(s) | 1438 |
| Bsh1236I | CGCG | 1 cut(s) | 1289 |
| BshFI | GGCC | 3 cut(s) | 191, 1646, 1858 |
| BshTI | ACCGGT | 1 cut(s) | 1273 |
| BsiHKAI | GWGCWC | 2 cut(s) | 623, 2018 |
| BsiSI | CCGG | 5 cut(s) | 216, 1035, 1274, 1772, 2109 |
| BsiWI | CGTACG | 1 cut(s) | 42 |
| BslFI | GGGAC | 2 cut(s) | 1225, 2035 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 118, 230, 750, 1232, 1747, 1877, 2059 |
| BsmAI | GTCTC | 2 cut(s) | 213, 1045 |
| BsmBI | CGTCTC | 1 cut(s) | 1045 |
| BsmFI | GGGAC | 2 cut(s) | 1225, 2035 |
| BsmI | GAATGC | 2 cut(s) | 1080, 2070 |
| BsnI | GGCC | 3 cut(s) | 191, 1646, 1858 |
| Bso31I | GGTCTC | 1 cut(s) | 213 |
| Bsp119I | TTCGAA | 2 cut(s) | 537, 1621 |
| Bsp1286I | GDGCHC | 3 cut(s) | 623, 780, 2018 |
| Bsp1407I | TGTACA | 1 cut(s) | 1627 |
| Bsp143I | GATC | 4 cut(s) | 293, 421, 1510, 1867 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1704 |
| BspACI | CCGC | 4 cut(s) | 50, 79, 1231, 1748 |
| BspANI | GGCC | 3 cut(s) | 191, 1646, 1858 |
| BspCNI | CTCAG | 8 cut(s) | 282, 331, 663, 721, 877, 1219, 1387, 1450 |
| BspFNI | CGCG | 1 cut(s) | 1289 |
| BspLI | GGNNCC | 1 cut(s) | 1475 |
| BspMAI | CTGCAG | 1 cut(s) | 1363 |
| BspMI | ACCTGC | 2 cut(s) | 1109, 1255 |
| BspPI | GGATC | 1 cut(s) | 1862 |
| BspQI | GCTCTTC | 1 cut(s) | 628 |
| BspT104I | TTCGAA | 2 cut(s) | 537, 1621 |
| BspTNI | GGTCTC | 1 cut(s) | 213 |
| BsrBI | CCGCTC | 1 cut(s) | 1231 |
| BsrDI | GCAATG | 2 cut(s) | 209, 838 |
| BsrFI | RCCGGY | 1 cut(s) | 1273 |
| BsrGI | TGTACA | 1 cut(s) | 1627 |
| BsrI | ACTGG | 6 cut(s) | 811, 1311, 1384, 1647, 2129, 2172 |
| BssAI | RCCGGY | 1 cut(s) | 1273 |
| BssECI | CCNNGG | 3 cut(s) | 781, 1075, 1372 |
| BssMI | GATC | 4 cut(s) | 293, 421, 1510, 1867 |
| BssNAI | GTATAC | 3 cut(s) | 718, 870, 1223 |
| BssNI | GRCGYC | 1 cut(s) | 1054 |
| BssSI | CACGAG | 1 cut(s) | 10 |
| BssT1I | CCWWGG | 2 cut(s) | 781, 1372 |
| Bst1107I | GTATAC | 3 cut(s) | 718, 870, 1223 |
| Bst2BI | CACGAG | 1 cut(s) | 10 |
| Bst2UI | CCWGG | 3 cut(s) | 17, 1076, 1433 |
| Bst4CI | ACNGT | 6 cut(s) | 47, 991, 1505, 2095, 2149, 2174 |
| Bst6I | CTCTTC | 2 cut(s) | 628, 1327 |
| BstACI | GRCGYC | 1 cut(s) | 1054 |
| BstAUI | TGTACA | 1 cut(s) | 1627 |
| BstBI | TTCGAA | 2 cut(s) | 537, 1621 |
| BstC8I | GCNNGC | 1 cut(s) | 850 |
| BstF5I | GGATG | 3 cut(s) | 1364, 1674, 2042 |
| BstFNI | CGCG | 1 cut(s) | 1289 |
| BstKTI | GATC | 4 cut(s) | 296, 424, 1513, 1870 |
| BstMAI | GTCTC | 2 cut(s) | 213, 1045 |
| BstMBI | GATC | 4 cut(s) | 293, 421, 1510, 1867 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 97, 340, 1256, 1265, 1286 |
| BstNI | CCWGG | 3 cut(s) | 17, 1076, 1433 |
| BstNSI | RCATGY | 1 cut(s) | 1016 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1811 |
| BstSCI | CCNGG | 5 cut(s) | 15, 1034, 1074, 1431, 2107 |
| BstSFI | CTRYAG | 3 cut(s) | 1298, 1359, 2145 |
| BstSLI | GKGCMC | 1 cut(s) | 2018 |
| BstUI | CGCG | 1 cut(s) | 1289 |
| BstV1I | GCAGC | 5 cut(s) | 158, 223, 1012, 1268, 1373 |
| BstV2I | GAAGAC | 3 cut(s) | 30, 732, 1811 |
| BstZ17I | GTATAC | 3 cut(s) | 718, 870, 1223 |
| BsuI | GTATCC | 1 cut(s) | 468 |
| BsuRI | GGCC | 3 cut(s) | 191, 1646, 1858 |
| BtsCI | GGATG | 3 cut(s) | 1364, 1674, 2042 |
| BtsI | GCAGTG | 2 cut(s) | 235, 403 |
| BtsIMutI | CAGTG | 8 cut(s) | 104, 171, 235, 403, 1270, 1304, 1455, 1855 |
| BveI | ACCTGC | 2 cut(s) | 1109, 1255 |
| Cac8I | GCNNGC | 1 cut(s) | 850 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1306 |
| Cfr10I | RCCGGY | 1 cut(s) | 1273 |
| Cfr13I | GGNCC | 5 cut(s) | 47, 189, 542, 785, 1244 |
| CpoI | CGGWCCG | 1 cut(s) | 47 |
| CseI | GACGC | 1 cut(s) | 1673 |
| CsiI | ACCWGGT | 1 cut(s) | 1431 |
| Csp6I | GTAC | 7 cut(s) | 43, 855, 1236, 1444, 1628, 1737, 2250 |
| CspAI | ACCGGT | 1 cut(s) | 1273 |
| CspI | CGGWCCG | 1 cut(s) | 47 |
| CviAII | CATG | 5 cut(s) | 225, 1013, 1163, 1783, 2047 |
| CviQI | GTAC | 7 cut(s) | 43, 855, 1236, 1444, 1628, 1737, 2250 |
| DpnI | GATC | 4 cut(s) | 295, 423, 1512, 1869 |
| DpnII | GATC | 4 cut(s) | 293, 421, 1510, 1867 |
| DraI | TTTAAA | 1 cut(s) | 1539 |
| DraIII | CACNNNGTG | 2 cut(s) | 12, 1325 |
| Eam1104I | CTCTTC | 2 cut(s) | 628, 1327 |
| EarI | CTCTTC | 2 cut(s) | 628, 1327 |
| Ecl136II | GAGCTC | 1 cut(s) | 621 |
| Eco130I | CCWWGG | 2 cut(s) | 781, 1372 |
| Eco24I | GRGCYC | 2 cut(s) | 623, 780 |
| Eco31I | GGTCTC | 1 cut(s) | 213 |
| Eco32I | GATATC | 1 cut(s) | 1686 |
| Eco47I | GGWCC | 4 cut(s) | 47, 542, 785, 1244 |
| Eco53kI | GAGCTC | 1 cut(s) | 621 |
| Eco57I | CTGAAG | 1 cut(s) | 47 |
| EcoICRI | GAGCTC | 1 cut(s) | 621 |
| EcoO109I | RGGNCCY | 1 cut(s) | 542 |
| EcoRI | GAATTC | 2 cut(s) | 160, 1967 |
| EcoRII | CCWGG | 3 cut(s) | 15, 1074, 1431 |
| EcoRV | GATATC | 1 cut(s) | 1686 |
| EcoT14I | CCWWGG | 2 cut(s) | 781, 1372 |
| EcoT38I | GRGCYC | 2 cut(s) | 623, 780 |
| ErhI | CCWWGG | 2 cut(s) | 781, 1372 |
| Esp3I | CGTCTC | 1 cut(s) | 1045 |
| FaeI | CATG | 5 cut(s) | 228, 1016, 1166, 1786, 2050 |
| FaqI | GGGAC | 2 cut(s) | 1225, 2035 |
| FatI | CATG | 5 cut(s) | 224, 1012, 1162, 1782, 2046 |
| FauI | CCCGC | 2 cut(s) | 1224, 1741 |
| FbaI | TGATCA | 1 cut(s) | 1510 |
| FblI | GTMKAC | 4 cut(s) | 21, 717, 869, 1222 |
| Fnu4HI | GCNGC | 5 cut(s) | 147, 237, 1026, 1257, 1362 |
| FokI | GGATG | 3 cut(s) | 1351, 1661, 2029 |
| FriOI | GRGCYC | 2 cut(s) | 623, 780 |
| Fsp4HI | GCNGC | 5 cut(s) | 147, 237, 1026, 1257, 1362 |
| FspBI | CTAG | 5 cut(s) | 344, 450, 1584, 1946, 2211 |
| GluI | GCNGC | 5 cut(s) | 147, 237, 1026, 1257, 1362 |
| GsuI | CTGGAG | 2 cut(s) | 1514, 1939 |
| HaeIII | GGCC | 3 cut(s) | 191, 1646, 1858 |
| HapII | CCGG | 5 cut(s) | 216, 1035, 1274, 1772, 2109 |
| HgaI | GACGC | 1 cut(s) | 1673 |
| Hin1I | GRCGYC | 1 cut(s) | 1054 |
| Hin1II | CATG | 5 cut(s) | 228, 1016, 1166, 1786, 2050 |
| HincII | GTYRAC | 1 cut(s) | 1554 |
| HindII | GTYRAC | 1 cut(s) | 1554 |
| HindIII | AAGCTT | 2 cut(s) | 503, 1706 |
| HinfI | GANTC | 7 cut(s) | 245, 403, 692, 908, 1449, 1610, 1952 |
| HpaII | CCGG | 5 cut(s) | 216, 1035, 1274, 1772, 2109 |
| HphI | GGTGA | 4 cut(s) | 1892, 2019, 2143, 2179 |
| Hpy188III | TCNNGA | 8 cut(s) | 922, 1493, 1508, 1604, 1838, 1901, 1956, 2073 |
| Hpy99I | CGWCG | 1 cut(s) | 1667 |
| HpyAV | CCTTC | 5 cut(s) | 533, 643, 711, 760, 1636 |
| HpyCH4III | ACNGT | 6 cut(s) | 47, 991, 1505, 2095, 2149, 2174 |
| HpyCH4IV | ACGT | 6 cut(s) | 375, 456, 737, 1054, 1558, 2193 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 97, 340, 1256, 1265, 1286 |
| HpySE526I | ACGT | 6 cut(s) | 375, 456, 737, 1054, 1558, 2193 |
| Hsp92I | GRCGYC | 1 cut(s) | 1054 |
| Hsp92II | CATG | 5 cut(s) | 228, 1016, 1166, 1786, 2050 |
| Ksp22I | TGATCA | 1 cut(s) | 1510 |
| Kzo9I | GATC | 4 cut(s) | 293, 421, 1510, 1867 |
| LguI | GCTCTTC | 1 cut(s) | 628 |
| LmnI | GCTCC | 6 cut(s) | 75, 81, 233, 618, 1136, 1479 |
| Lsp1109I | GCAGC | 5 cut(s) | 158, 223, 1012, 1268, 1373 |
| LweI | GCATC | 1 cut(s) | 1373 |
| MabI | ACCWGGT | 1 cut(s) | 1431 |
| MaeI | CTAG | 5 cut(s) | 344, 450, 1584, 1946, 2211 |
| MaeII | ACGT | 6 cut(s) | 375, 456, 737, 1054, 1558, 2193 |
| MaeIII | GTNAC | 9 cut(s) | 802, 980, 1390, 2086, 2095, 2111, 2149, 2185, 2194 |
| MalI | GATC | 4 cut(s) | 295, 423, 1512, 1869 |
| MbiI | CCGCTC | 1 cut(s) | 1231 |
| MboI | GATC | 4 cut(s) | 293, 421, 1510, 1867 |
| MfeI | CAATTG | 3 cut(s) | 1352, 1755, 1916 |
| MhlI | GDGCHC | 3 cut(s) | 623, 780, 2018 |
| MlyI | GAGTC | 2 cut(s) | 239, 1946 |
| MmeI | TCCRAC | 1 cut(s) | 817 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2068 |
| MseI | TTAA | 6 cut(s) | 353, 1467, 1538, 1563, 1592, 2141 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1635, 2192 |
| MspI | CCGG | 5 cut(s) | 216, 1035, 1274, 1772, 2109 |
| MspR9I | CCNGG | 5 cut(s) | 17, 1036, 1076, 1433, 2109 |
| MunI | CAATTG | 3 cut(s) | 1352, 1755, 1916 |
| Mva1269I | GAATGC | 2 cut(s) | 1080, 2070 |
| MvaI | CCWGG | 3 cut(s) | 17, 1076, 1433 |
| MvnI | CGCG | 1 cut(s) | 1289 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 97, 340, 1256, 1265, 1286 |
| NciI | CCSGG | 2 cut(s) | 1036, 2109 |
| NdeII | GATC | 4 cut(s) | 293, 421, 1510, 1867 |
| NlaIII | CATG | 5 cut(s) | 228, 1016, 1166, 1786, 2050 |
| NlaIV | GGNNCC | 1 cut(s) | 1475 |
| NmuCI | GTSAC | 5 cut(s) | 802, 2086, 2149, 2185, 2194 |
| NspI | RCATGY | 1 cut(s) | 1016 |
| NspV | TTCGAA | 2 cut(s) | 537, 1621 |
| OliI | CACNNNNGTG | 1 cut(s) | 2192 |
| PciI | ACATGT | 1 cut(s) | 1012 |
| PciSI | GCTCTTC | 1 cut(s) | 628 |
| PctI | GAATGC | 2 cut(s) | 1080, 2070 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2068 |
| PfeI | GAWTC | 5 cut(s) | 403, 692, 908, 1449, 1610 |
| Pfl23II | CGTACG | 1 cut(s) | 42 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 230 |
| PinAI | ACCGGT | 1 cut(s) | 1273 |
| PkrI | GCNGC | 5 cut(s) | 148, 238, 1027, 1258, 1363 |
| PleI | GAGTC | 2 cut(s) | 239, 1946 |
| PpsI | GAGTC | 2 cut(s) | 239, 1946 |
| PpuMI | RGGWCCY | 1 cut(s) | 542 |
| PscI | ACATGT | 1 cut(s) | 1012 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1811 |
| Psp124BI | GAGCTC | 1 cut(s) | 623 |
| Psp1406I | AACGTT | 1 cut(s) | 456 |
| Psp5II | RGGWCCY | 1 cut(s) | 542 |
| Psp6I | CCWGG | 3 cut(s) | 15, 1074, 1431 |
| PspGI | CCWGG | 3 cut(s) | 15, 1074, 1431 |
| PspLI | CGTACG | 1 cut(s) | 42 |
| PspN4I | GGNNCC | 1 cut(s) | 1475 |
| PspPI | GGNCC | 5 cut(s) | 47, 189, 542, 785, 1244 |
| PspPPI | RGGWCCY | 1 cut(s) | 542 |
| PstI | CTGCAG | 1 cut(s) | 1363 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1306 |
| RsaI | GTAC | 7 cut(s) | 44, 856, 1237, 1445, 1629, 1738, 2251 |
| RsaNI | GTAC | 7 cut(s) | 43, 855, 1236, 1444, 1628, 1737, 2250 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1635, 2192 |
| Rsr2I | CGGWCCG | 1 cut(s) | 47 |
| RsrII | CGGWCCG | 1 cut(s) | 47 |
| SacI | GAGCTC | 1 cut(s) | 623 |
| SapI | GCTCTTC | 1 cut(s) | 628 |
| SaqAI | TTAA | 6 cut(s) | 353, 1467, 1538, 1563, 1592, 2141 |
| SatI | GCNGC | 5 cut(s) | 147, 237, 1026, 1257, 1362 |
| Sau3AI | GATC | 4 cut(s) | 293, 421, 1510, 1867 |
| Sau96I | GGNCC | 5 cut(s) | 47, 189, 542, 785, 1244 |
| SchI | GAGTC | 2 cut(s) | 239, 1946 |
| ScrFI | CCNGG | 5 cut(s) | 17, 1036, 1076, 1433, 2109 |
| SduI | GDGCHC | 3 cut(s) | 623, 780, 2018 |
| SexAI | ACCWGGT | 1 cut(s) | 1431 |
| SfaNI | GCATC | 1 cut(s) | 1373 |
| SfcI | CTRYAG | 3 cut(s) | 1298, 1359, 2145 |
| SfuI | TTCGAA | 2 cut(s) | 537, 1621 |
| SinI | GGWCC | 4 cut(s) | 47, 542, 785, 1244 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1635, 2192 |
| SmlI | CTYRAG | 6 cut(s) | 59, 86, 116, 514, 971, 998 |
| SmoI | CTYRAG | 6 cut(s) | 59, 86, 116, 514, 971, 998 |
| SpeI | ACTAGT | 1 cut(s) | 1945 |
| SsiI | CCGC | 4 cut(s) | 50, 79, 1231, 1748 |
| SspI | AATATT | 3 cut(s) | 184, 685, 1654 |
| SspMI | CTAG | 5 cut(s) | 344, 450, 1584, 1946, 2211 |
| SstI | GAGCTC | 1 cut(s) | 623 |
| StyD4I | CCNGG | 5 cut(s) | 15, 1034, 1074, 1431, 2107 |
| StyI | CCWWGG | 2 cut(s) | 781, 1372 |
| TaaI | ACNGT | 6 cut(s) | 47, 991, 1505, 2095, 2149, 2174 |
| TaiI | ACGT | 6 cut(s) | 378, 459, 740, 1057, 1561, 2196 |
| TaqI | TCGA | 6 cut(s) | 537, 1062, 1613, 1621, 1662, 1902 |
| TatI | WGTACW | 2 cut(s) | 1627, 2249 |
| TfiI | GAWTC | 5 cut(s) | 403, 692, 908, 1449, 1610 |
| Tru1I | TTAA | 6 cut(s) | 353, 1467, 1538, 1563, 1592, 2141 |
| Tru9I | TTAA | 6 cut(s) | 353, 1467, 1538, 1563, 1592, 2141 |
| TscAI | CASTG | 8 cut(s) | 111, 171, 235, 403, 1270, 1311, 1462, 1855 |
| TseFI | GTSAC | 5 cut(s) | 802, 2086, 2149, 2185, 2194 |
| TseI | GCWGC | 5 cut(s) | 146, 236, 1025, 1256, 1361 |
| Tsp45I | GTSAC | 5 cut(s) | 802, 2086, 2149, 2185, 2194 |
| TspDTI | ATGAA | 5 cut(s) | 475, 593, 662, 783, 933 |
| TspGWI | ACGGA | 1 cut(s) | 1461 |
| TspRI | CASTG | 8 cut(s) | 111, 171, 235, 403, 1270, 1311, 1462, 1855 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 230 |
| VneI | GTGCAC | 1 cut(s) | 2014 |
| VpaK11BI | GGWCC | 4 cut(s) | 47, 542, 785, 1244 |
| XapI | RAATTY | 8 cut(s) | 160, 349, 557, 1040, 1096, 1861, 1967, 2129 |
| XceI | RCATGY | 1 cut(s) | 1016 |
| XmiI | GTMKAC | 4 cut(s) | 21, 717, 869, 1222 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2068 |
| XspI | CTAG | 5 cut(s) | 344, 450, 1584, 1946, 2211 |
| ZraI | GACGTC | 1 cut(s) | 1055 |