RchiOBHm_Chr2g0124231
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000088
Physical Location & Seq
Forward (+)
37586692 .. 37595005
8314 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49645

Sequence Viewer

Length: 3075 bp
ATGAGTATTTACCTCTGTCGAGGATGCGGCAGTTTCTCTATCAATGTTGAACTTTTTGCGCGAGCTCTGAGCTCAATCTTCCAGCAATGGCAGATTGAGGCCAAATGGAACACAACCAGAGATCCATGCAGTGGAGAGGCCATTGACGACTCCATCGAAATCTACAACGAAGCTTACAACCCTTTCGCCAAATGCAACTGTTCAAACTCCGCTTGCCACATTATCCAACTGAAAGTTTCTACATTGAAGGTTGTTGGCCTAATTCCGGAACAGCTATGGACTTTGAAGTTTCTCACTCATCTCGATTTAGGTCACAATCACTTGACAGGACCTCTCTCTGCATCAATTGGAAATCTGAGCAGCTTGGAATATTTGAGCTTAGGCCATAACATGTTATCAGGCCAGCTACCAAAGGAACTCGGAAATCTCTCTAATTTGACATTCTTGGCTATTGGGACTATGAACCTTTCCGGTGGTCTACCACATGAGCTAGGGAATTTATTAAATTTACAACAGATTTACTTGGATAGTTCTGGAGTTAGCGGTGAGATTCCACCGACGTTTGCTAATCTACGAAACTTGACCATATTCCGGGTGTCAGACACAGAACTCTCTGGCAGGATACCTGACTTCGTAGGATATTGGTCAAAGCTTACTTCCTTGGTATTTCAAGGGAACTCTTTTGAAGGTCCTATACCAGCTACACTGTCCAATCTGACTTCTCTTGAGGAGCTGAGAATTAGTGATATAATATCTACTGCCAGTGGGAACTCTTCTCTTGGATTTATTAAGGATATGAAGTCAAGATCTCTAAAAGTCCTAGTGCTGCGAAATAACAATATTTCTGATTCAATTCCTTCTAATATTGGAGAGTTGCAAAGTTTGTATCACCTGGATTTAAGCTTCAACAATTTAGCAGGAGTAATACCGCCTTCCCTGTTTAATTTGCCTTCGCTCAAAGCCCTGTTTCTTGGAAACAACAAGTTGAGTGGTAACCTTCCTGAACATAAAAGCTCGTCTCTTGTCAATGTAGATCTGTCTTACAATAATCTAGTAGGGTCAAGCTTTCCTTCTTGGGTGAACGAAGACTTACAGCTTAATTTAGTTGCCAACAACTTCAGCACAGAAAATTCAAACGGCAGATCTTTGCCTTCAGGGTTGAAATGCCTCCAAAGGAACTTTCCTTGCAATGTTAACCCTGGAATCCATCCTTACATTGGGATTAAATGTGGTGGTCCTCAGTTCACGTCTTCCAATGGGATTGTGTATGATCAGGAAAATGAGACCCTAGGTCCAGCTACATATTTTGTGACTGGCACAAGCAGATGGGGAGTTAGCAATGTTGGTATTAAAACAGAGTATCAGGATGGATATATCACGGGAAAAATTCCTGAGTATACAGTTTCCTCGTCATCTCAATTCACGAATACTCCAGATCCTGGGCTATTCCGGACTGCAAGGATCTCTGCTTCATCACTTAGATACCATGGCTTGGGGCTGGAGAATGGTAACTACACGGTCAAACTTCAGTTTGCAGAACAAAAAATCCCAGATACTCCAAAAAATCTTGGGAGACGTGTATTTGATATATATATCCAGGGAATTCTAGTTTTGAAGGATTTTGATATACGAAAGGAAGCAGGTGGGAAATCTTTGCAAGCCGTTCAGAAGGAATATAAGGCCCAGGTTTCACAGAACTACCTTGAAATTCATCTCTTTTGGGCTGGAAAGGGGACTTGCTGCGTACCTGAGGTTGGTACTTATGGACCTGTTATTTCAGCCATCAGTGCTACACCAGATTTCCTACCTACTATCGTTCCGAACCATCCAACTAGTAAGAAGAATAGGACGGGGCTGATTGTGGGGATTGTTGTTGGTGGTGGAGTACTTTTGATTCTATTGTTGTTGTTTTTCTGTATAGTTCAAAGAAGGAAAAGGATTAACACCAGTAAAAATGAAGATCTGTTGGGAATAGATATTGGACCACTCACTTTCAGTTTTTCAGAGCTAAAGACAGCTACAGATGACTTTAGTCCAGCGAATAAGATTGGAGAGGGAGGATTTGGACCTGTCTATAAAGGTACTCTTAATGATGGAAGAGTAATTGCTGTGAAGCAATTGTCTGCAGCATCCCAGCAAGGAAAAAATGAGTTTGTAGCTGAAATTGCCACCATATCTGCCGTGCGACACAATAACCTAGTGAATTTGCATGGATTCTGCACTGAGGGAGTTAAACGGCTCCTTGTCTATGAGTATCTGGAAAACAAGAGTCTTGATCATGCATTATTTGGAAAAAGAAGTTCGAATCTTGATTGGTCATCAAGATTTGATATATGCTTGGGCGTGGCTAAAGGTTTGACTTATCTCCATGAGGAATCAAGGGTTCGGATTGTACACAGAGATGTGAAGGCCAGTAATATTCTGCTCGACTCTAATCTCATCCCCAAAATATCAGATTTCGGTTTGGCCAAGCTTTATGATGATAAAAAGACCCACATCAGTACTCGTGTTGCGGGAACAATAGGGTATCTTGCACCGGAATATGCCATGCGTGGACACTTGACACAGAAGACTGATGTGTTTGCCTTTGGTGTTCTTGCTCTAGAAATTGTCAGTGGTAGGCCAAATTCTGACCGGAGCTTGGATGGCGAGATGATTTATCTTCTCGAATGGGCTTGGCACTTGCATGAAAACAAACGCGAAGTTGAACTAGTGGACTCTAGGTTATCAGAATTCAATGAGGAAGAGGCAAGACGAATAACCAAGATAGGACTTCTGTGCACTCAAACATCACCAATGCAACGACCACCGATGTCGCGTGTGGTGGGAATGCTTTCAGGAGATATTGAAGTAATGACAGTAACTTCAAAGCCTAGTTACTTGACAGACTGGGAATTTGACGATACAAGTACTTTCACCAGTCCAGCCACTGATATGTCAGCAAAAGGAACTGATCCGAGCTTTTACAACTCACCGGCGAGTACAAGCACTTTGGGGGATCCAGGGCAGCTTTCTAAAAATGCCACGGATGCCTTGCTTCATAATGTTGTTGATACCCAATCCCTACCGACTTAA

Protein Analysis

1024

Amino Acids

112.25

Weight (kDa)

6.29

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 86 - 176 8.5e-08 Leucine-rich repeat region
LRR_14 PF23598 163 - 252 1.5e-07 Leucine-rich repeat region
LRR_8 PF13855 269 - 329 3.9e-08 Leucine rich repeat
Malectin PF11721 407 - 595 3.9e-42 Malectin domain
Pkinase PF00069 680 - 878 2.6e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 681 - 943 4.2e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1631
Acc36I ACCTGC 1 cut(s) 1631
AccB7I CCANNNNNTGG 3 cut(s) 131, 1439, 1492
AccI GTMKAC 2 cut(s) 478, 1397
AccII CGCG 3 cut(s) 61, 2700, 2818
AccIII TCCGGA 2 cut(s) 265, 1449
AciI CCGC 5 cut(s) 27, 210, 543, 929, 2513
AclWI GGATC 6 cut(s) 116, 1430, 1469, 2948, 2993, 3006
AcoI YGGCCR 1 cut(s) 2466
AcuI CTGAAG 3 cut(s) 1102, 1137, 1511
AfaI GTAC 8 cut(s) 1746, 1759, 1887, 2083, 2394, 2503, 2911, 2983
AfiI CCNNNNNNNGG 8 cut(s) 131, 265, 591, 1217, 1439, 1492, 1754, 2641
AflIII ACRYGT 2 cut(s) 390, 1576
AhdI GACNNNNNGTC 1 cut(s) 1290
AhlI ACTAGT 2 cut(s) 1832, 2710
AjiI CACGTC 2 cut(s) 1248, 1577
AjnI CCWGG 6 cut(s) 891, 1198, 1438, 1596, 1683, 3001
AjuI GAANNNNNNNTTGG 2 cut(s) 1164, 1196
AloI GAACNNNNNNTCC 2 cut(s) 1530, 1562
Alw21I GWGCWC 3 cut(s) 67, 74, 2783
Alw26I GTCTC 3 cut(s) 1023, 1277, 1567
Alw44I GTGCAC 1 cut(s) 2779
AlwI GGATC 6 cut(s) 116, 1430, 1469, 2948, 2993, 3006
AlwNI CAGNNNCTG 2 cut(s) 1439, 2930
Aor13HI TCCGGA 2 cut(s) 265, 1449
AoxI GGCC 9 cut(s) 99, 138, 256, 382, 400, 1680, 2409, 2466, 2621
ApaLI GTGCAC 1 cut(s) 2779
ApeKI GCWGC 5 cut(s) 360, 826, 1740, 2126, 3007
ArsI GACNNNNNNTTYG 2 cut(s) 2879, 2911
Asp700I GAANNNNTTC 1 cut(s) 2833
AspA2I CCTAGG 1 cut(s) 1288
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 8 cut(s) 329, 689, 1235, 1292, 1681, 1766, 1982, 2066
AsuC2I CCSGG 1 cut(s) 593
AsuHPI GGTGA 6 cut(s) 557, 881, 1090, 2784, 2908, 2964
AsuII TTCGAA 1 cut(s) 2303
AvaII GGWCC 7 cut(s) 329, 689, 1235, 1292, 1766, 1982, 2066
AvrII CCTAGG 1 cut(s) 1288
AxyI CCTNAGG 1 cut(s) 1749
BaeGI GKGCMC 1 cut(s) 2783
BalI TGGCCA 1 cut(s) 2468
BamHI GGATCC 1 cut(s) 2998
BanII GRGCYC 2 cut(s) 67, 74
BarI GAAGNNNNNNTAC 2 cut(s) 678, 710
BauI CACGAG 1 cut(s) 2505
BbsI GAAGAC 3 cut(s) 1092, 1242, 2576
Bbv12I GWGCWC 3 cut(s) 67, 74, 2783
BbvI GCAGC 5 cut(s) 372, 813, 1727, 2138, 3019
BccI CCATC 8 cut(s) 161, 1215, 1320, 1361, 1790, 1833, 2087, 2639
BceAI ACGGC 4 cut(s) 1153, 1646, 2165, 2252
BciT130I CCWGG 6 cut(s) 893, 1200, 1440, 1598, 1685, 3003
BciVI GTATCC 1 cut(s) 615
BclI TGATCA 2 cut(s) 1270, 2275
BcnI CCSGG 1 cut(s) 593
BcoDI GTCTC 3 cut(s) 1023, 1277, 1567
BcuI ACTAGT 2 cut(s) 1832, 2710
BfmI CTRYAG 2 cut(s) 2019, 2124
BfuAI ACCTGC 1 cut(s) 1631
BfuI GTATCC 1 cut(s) 615
BglII AGATCT 4 cut(s) 806, 1033, 1142, 1960
BisI GCNGC 6 cut(s) 28, 361, 827, 1741, 2127, 3008
BlnI CCTAGG 1 cut(s) 1288
BlsI GCNGC 6 cut(s) 29, 362, 828, 1742, 2128, 3009
BmcAI AGTACT 3 cut(s) 1887, 2503, 2911
Bme1390I CCNGG 7 cut(s) 593, 893, 1200, 1440, 1598, 1685, 3003
Bme18I GGWCC 7 cut(s) 329, 689, 1235, 1292, 1766, 1982, 2066
BmeRI GACNNNNNGTC 1 cut(s) 1290
BmgBI CACGTC 2 cut(s) 1248, 1577
BmgT120I GGNCC 8 cut(s) 329, 689, 1235, 1292, 1681, 1766, 1982, 2066
BmiI GGNNCC 2 cut(s) 2240, 3000
BmrFI CCNGG 7 cut(s) 593, 893, 1200, 1440, 1598, 1685, 3003
BmrI ACTGGG 1 cut(s) 2899
BmsI GCATC 4 cut(s) 14, 350, 2138, 3019
BmuI ACTGGG 1 cut(s) 2899
BoxI GACNNNNGTC 1 cut(s) 2031
BpiI GAAGAC 3 cut(s) 1092, 1242, 2576
BpmI CTGGAG 3 cut(s) 555, 1416, 1520
Bpu10I CCTNAGC 1 cut(s) 379
Bpu14I TTCGAA 1 cut(s) 2303
BpuEI CTTGAG 1 cut(s) 746
BpuMI CCSGG 1 cut(s) 593
BsaI GGTCTC 1 cut(s) 1277
BsaJI CCNNGG 9 cut(s) 660, 1198, 1288, 1439, 1486, 1597, 1683, 3002, 3024
BsaWI WCCGGW 5 cut(s) 265, 470, 1449, 2536, 2634
BsaXI ACNNNNNCTCC 2 cut(s) 1414, 1444
Bsc4I CCNNNNNNNGG 8 cut(s) 131, 265, 591, 1217, 1439, 1492, 1754, 2641
Bse118I RCCGGY 1 cut(s) 2974
Bse1I ACTGG 6 cut(s) 762, 1318, 1947, 2412, 2894, 2919
Bse21I CCTNAGG 1 cut(s) 1749
Bse3DI GCAATG 3 cut(s) 92, 1195, 1345
BseAI TCCGGA 2 cut(s) 265, 1449
BseBI CCWGG 6 cut(s) 893, 1200, 1440, 1598, 1685, 3003
BseDI CCNNGG 9 cut(s) 660, 1198, 1288, 1439, 1486, 1597, 1683, 3002, 3024
BseGI GGATG 8 cut(s) 29, 1207, 1372, 1825, 2129, 2439, 2650, 3034
BseLI CCNNNNNNNGG 8 cut(s) 131, 265, 591, 1217, 1439, 1492, 1754, 2641
BseMI GCAATG 3 cut(s) 92, 1195, 1345
BseMII CTCAG 7 cut(s) 59, 347, 725, 1253, 1383, 1740, 2214
BseNI ACTGG 6 cut(s) 762, 1318, 1947, 2412, 2894, 2919
BseRI GAGGAG 1 cut(s) 743
BseSI GKGCMC 1 cut(s) 2783
BseXI GCAGC 5 cut(s) 372, 813, 1727, 2138, 3019
BseYI CCCAGC 1 cut(s) 2133
BsgI GTGCAG 1 cut(s) 2203
Bsh1236I CGCG 3 cut(s) 61, 2700, 2818
BshFI GGCC 9 cut(s) 101, 140, 258, 384, 402, 1682, 2411, 2468, 2623
BsiHKAI GWGCWC 3 cut(s) 67, 74, 2783
BsiSI CCGG 7 cut(s) 266, 471, 592, 1450, 2537, 2635, 2975
BslFI GGGAC 2 cut(s) 469, 1747
BslI CCNNNNNNNGG 8 cut(s) 131, 265, 591, 1217, 1439, 1492, 1754, 2641
BsmAI GTCTC 3 cut(s) 1023, 1277, 1567
BsmBI CGTCTC 2 cut(s) 1023, 1567
BsmFI GGGAC 2 cut(s) 469, 1747
BsmI GAATGC 1 cut(s) 2835
BsnI GGCC 9 cut(s) 101, 140, 258, 384, 402, 1682, 2411, 2468, 2623
Bso31I GGTCTC 1 cut(s) 1277
Bsp119I TTCGAA 1 cut(s) 2303
Bsp1286I GDGCHC 3 cut(s) 67, 74, 2783
Bsp13I TCCGGA 2 cut(s) 265, 1449
Bsp1407I TGTACA 1 cut(s) 2392
Bsp19I CCATGG 1 cut(s) 1486
BspACI CCGC 5 cut(s) 27, 210, 543, 929, 2513
BspANI GGCC 9 cut(s) 101, 140, 258, 384, 402, 1682, 2411, 2468, 2623
BspCNI CTCAG 7 cut(s) 60, 348, 726, 1252, 1384, 1741, 2215
BspEI TCCGGA 2 cut(s) 265, 1449
BspFNI CGCG 3 cut(s) 61, 2700, 2818
BspLI GGNNCC 2 cut(s) 2240, 3000
BspMAI CTGCAG 1 cut(s) 2128
BspMI ACCTGC 1 cut(s) 1631
BspPI GGATC 6 cut(s) 116, 1430, 1469, 2948, 2993, 3006
BspT104I TTCGAA 1 cut(s) 2303
BspTNI GGTCTC 1 cut(s) 1277
BsrDI GCAATG 3 cut(s) 92, 1195, 1345
BsrFI RCCGGY 1 cut(s) 2974
BsrGI TGTACA 1 cut(s) 2392
BsrI ACTGG 6 cut(s) 762, 1318, 1947, 2412, 2894, 2919
BssAI RCCGGY 1 cut(s) 2974
BssECI CCNNGG 9 cut(s) 660, 1198, 1288, 1439, 1486, 1597, 1683, 3002, 3024
BssNAI GTATAC 1 cut(s) 1398
BssSI CACGAG 1 cut(s) 2505
BssT1I CCWWGG 3 cut(s) 660, 1288, 1486
Bst1107I GTATAC 1 cut(s) 1398
Bst2BI CACGAG 1 cut(s) 2505
Bst2UI CCWGG 6 cut(s) 893, 1200, 1440, 1598, 1685, 3003
Bst4CI ACNGT 5 cut(s) 200, 708, 1402, 1519, 2860
Bst6I CTCTTC 3 cut(s) 778, 2092, 2739
BstAUI TGTACA 1 cut(s) 2392
BstBI TTCGAA 1 cut(s) 2303
BstC8I GCNNGC 4 cut(s) 63, 214, 404, 1659
BstDEI CTNAG 9 cut(s) 68, 356, 379, 734, 1239, 1392, 1478, 1749, 2223
BstDSI CCRYGG 2 cut(s) 1486, 3024
BstEII GGTNACC 1 cut(s) 992
BstF5I GGATG 8 cut(s) 29, 1207, 1372, 1825, 2129, 2439, 2650, 3034
BstFNI CGCG 3 cut(s) 61, 2700, 2818
BstHHI GCGC 1 cut(s) 61
BstMAI GTCTC 3 cut(s) 1023, 1277, 1567
BstMWI GCNNNNNNNGC 4 cut(s) 1787, 2165, 2646, 3029
BstNI CCWGG 6 cut(s) 893, 1200, 1440, 1598, 1685, 3003
BstNSI RCATGY 1 cut(s) 394
BstPAI GACNNNNGTC 1 cut(s) 2031
BstPI GGTNACC 1 cut(s) 992
BstSCI CCNGG 7 cut(s) 591, 891, 1198, 1438, 1596, 1683, 3001
BstSFI CTRYAG 2 cut(s) 2019, 2124
BstSLI GKGCMC 1 cut(s) 2783
BstUI CGCG 3 cut(s) 61, 2700, 2818
BstV1I GCAGC 5 cut(s) 372, 813, 1727, 2138, 3019
BstV2I GAAGAC 3 cut(s) 1092, 1242, 2576
BstX2I RGATCY 8 cut(s) 121, 806, 1033, 1142, 1435, 1461, 1960, 2998
BstYI RGATCY 8 cut(s) 121, 806, 1033, 1142, 1435, 1461, 1960, 2998
BstZ17I GTATAC 1 cut(s) 1398
Bsu36I CCTNAGG 1 cut(s) 1749
BsuI GTATCC 1 cut(s) 615
BsuRI GGCC 9 cut(s) 101, 140, 258, 384, 402, 1682, 2411, 2468, 2623
BtgI CCRYGG 2 cut(s) 1486, 3024
BtrI CACGTC 2 cut(s) 1248, 1577
BtsCI GGATG 8 cut(s) 29, 1207, 1372, 1825, 2129, 2439, 2650, 3034
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 7 cut(s) 136, 704, 769, 1792, 2220, 2620, 2928
BveI ACCTGC 1 cut(s) 1631
Cac8I GCNNGC 4 cut(s) 63, 214, 404, 1659
CaiI CAGNNNCTG 2 cut(s) 1439, 2930
CfoI GCGC 1 cut(s) 61
Cfr10I RCCGGY 1 cut(s) 2974
Cfr13I GGNCC 8 cut(s) 329, 689, 1235, 1292, 1681, 1766, 1982, 2066
Csp6I GTAC 8 cut(s) 1745, 1758, 1886, 2082, 2393, 2502, 2910, 2982
CspCI CAANNNNNGTGG 2 cut(s) 970, 1005
CviAII CATG 9 cut(s) 126, 391, 485, 1487, 2210, 2279, 2369, 2548, 2687
CviQI GTAC 8 cut(s) 1745, 1758, 1886, 2082, 2393, 2502, 2910, 2982
DdeI CTNAG 9 cut(s) 68, 356, 379, 734, 1239, 1392, 1478, 1749, 2223
DriI GACNNNNNGTC 1 cut(s) 1290
EaeI YGGCCR 1 cut(s) 2466
Eam1104I CTCTTC 3 cut(s) 778, 2092, 2739
Eam1105I GACNNNNNGTC 1 cut(s) 1290
EarI CTCTTC 3 cut(s) 778, 2092, 2739
Ecl136II GAGCTC 2 cut(s) 65, 72
Eco130I CCWWGG 3 cut(s) 660, 1288, 1486
Eco24I GRGCYC 2 cut(s) 67, 74
Eco31I GGTCTC 1 cut(s) 1277
Eco47I GGWCC 7 cut(s) 329, 689, 1235, 1292, 1766, 1982, 2066
Eco53kI GAGCTC 2 cut(s) 65, 72
Eco57I CTGAAG 3 cut(s) 1102, 1137, 1511
Eco81I CCTNAGG 1 cut(s) 1749
Eco91I GGTNACC 1 cut(s) 992
EcoICRI GAGCTC 2 cut(s) 65, 72
EcoO109I RGGNCCY 2 cut(s) 329, 689
EcoO65I GGTNACC 1 cut(s) 992
EcoRI GAATTC 2 cut(s) 1602, 2732
EcoRII CCWGG 6 cut(s) 891, 1198, 1438, 1596, 1683, 3001
EcoT14I CCWWGG 3 cut(s) 660, 1288, 1486
EcoT22I ATGCAT 1 cut(s) 2284
EcoT38I GRGCYC 2 cut(s) 67, 74
ErhI CCWWGG 3 cut(s) 660, 1288, 1486
Esp3I CGTCTC 2 cut(s) 1023, 1567
FaeI CATG 9 cut(s) 129, 394, 488, 1490, 2213, 2282, 2372, 2551, 2690
FalI AAGNNNNNCTT 4 cut(s) 1054, 1086, 2070, 2102
FaqI GGGAC 2 cut(s) 469, 1747
FatI CATG 9 cut(s) 125, 390, 484, 1486, 2209, 2278, 2368, 2547, 2686
FauI CCCGC 1 cut(s) 2506
FbaI TGATCA 2 cut(s) 1270, 2275
FblI GTMKAC 2 cut(s) 478, 1397
Fnu4HI GCNGC 6 cut(s) 28, 361, 827, 1741, 2127, 3008
FokI GGATG 8 cut(s) 36, 1194, 1379, 1812, 2116, 2426, 2657, 3041
FriOI GRGCYC 2 cut(s) 67, 74
Fsp4HI GCNGC 6 cut(s) 28, 361, 827, 1741, 2127, 3008
GlaI GCGC 1 cut(s) 60
GluI GCNGC 6 cut(s) 28, 361, 827, 1741, 2127, 3008
GsaI CCCAGC 1 cut(s) 2137
GsuI CTGGAG 3 cut(s) 555, 1416, 1520
HaeIII GGCC 9 cut(s) 101, 140, 258, 384, 402, 1682, 2411, 2468, 2623
HapII CCGG 7 cut(s) 266, 471, 592, 1450, 2537, 2635, 2975
HhaI GCGC 1 cut(s) 61
Hin1II CATG 9 cut(s) 129, 394, 488, 1490, 2213, 2282, 2372, 2551, 2690
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HincII GTYRAC 1 cut(s) 1195
HindII GTYRAC 1 cut(s) 1195
HindIII AAGCTT 5 cut(s) 171, 650, 901, 1063, 2471
HpaI GTTAAC 1 cut(s) 1195
HpaII CCGG 7 cut(s) 266, 471, 592, 1450, 2537, 2635, 2975
HphI GGTGA 6 cut(s) 557, 881, 1090, 2784, 2908, 2964
Hpy166II GTNNAC 9 cut(s) 479, 1081, 1195, 1245, 1398, 2395, 2555, 2716, 2781
Hpy8I GTNNAC 9 cut(s) 479, 1081, 1195, 1245, 1398, 2395, 2555, 2716, 2781
Hpy99I CGWCG 1 cut(s) 562
HpyCH4III ACNGT 5 cut(s) 200, 708, 1402, 1519, 2860
HpyCH4IV ACGT 3 cut(s) 560, 1247, 1576
HpyF10VI GCNNNNNNNGC 4 cut(s) 1787, 2165, 2646, 3029
HpyF3I CTNAG 9 cut(s) 68, 356, 379, 734, 1239, 1392, 1478, 1749, 2223
HpySE526I ACGT 3 cut(s) 560, 1247, 1576
Hsp92II CATG 9 cut(s) 129, 394, 488, 1490, 2213, 2282, 2372, 2551, 2690
HspAI GCGC 1 cut(s) 59
Kpn2I TCCGGA 2 cut(s) 265, 1449
Ksp22I TGATCA 2 cut(s) 1270, 2275
KspAI GTTAAC 1 cut(s) 1195
LmnI GCTCC 3 cut(s) 730, 2244, 2637
Lsp1109I GCAGC 5 cut(s) 372, 813, 1727, 2138, 3019
LweI GCATC 4 cut(s) 14, 350, 2138, 3019
MaeII ACGT 3 cut(s) 560, 1247, 1576
MaeIII GTNAC 6 cut(s) 311, 992, 1309, 1508, 2860, 2876
MfeI CAATTG 2 cut(s) 345, 2117
MflI RGATCY 8 cut(s) 121, 806, 1033, 1142, 1435, 1461, 1960, 2998
MhlI GDGCHC 3 cut(s) 67, 74, 2783
MlsI TGGCCA 1 cut(s) 2468
MluNI TGGCCA 1 cut(s) 2468
MlyI GAGTC 4 cut(s) 143, 2278, 2423, 2711
MmeI TCCRAC 2 cut(s) 250, 1853
Mox20I TGGCCA 1 cut(s) 2468
Mph1103I ATGCAT 1 cut(s) 2284
MroI TCCGGA 2 cut(s) 265, 1449
MroXI GAANNNNTTC 1 cut(s) 2833
MscI TGGCCA 1 cut(s) 2468
MslI CAYNNNNRTG 3 cut(s) 2400, 2685, 2933
Msp20I TGGCCA 1 cut(s) 2468
MspI CCGG 7 cut(s) 266, 471, 592, 1450, 2537, 2635, 2975
MspR9I CCNGG 7 cut(s) 593, 893, 1200, 1440, 1598, 1685, 3003
MunI CAATTG 2 cut(s) 345, 2117
Mva1269I GAATGC 1 cut(s) 2835
MvaI CCWGG 6 cut(s) 893, 1200, 1440, 1598, 1685, 3003
MvnI CGCG 3 cut(s) 61, 2700, 2818
MwoI GCNNNNNNNGC 4 cut(s) 1787, 2165, 2646, 3029
NciI CCSGG 1 cut(s) 593
NcoI CCATGG 1 cut(s) 1486
NlaIII CATG 9 cut(s) 129, 394, 488, 1490, 2213, 2282, 2372, 2551, 2690
NlaIV GGNNCC 2 cut(s) 2240, 3000
NmuCI GTSAC 2 cut(s) 311, 1309
NsiI ATGCAT 1 cut(s) 2284
NspI RCATGY 1 cut(s) 394
NspV TTCGAA 1 cut(s) 2303
PaqCI CACCTGC 1 cut(s) 1631
PciI ACATGT 1 cut(s) 390
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PctI GAATGC 1 cut(s) 2835
PdmI GAANNNNTTC 1 cut(s) 2833
PfeI GAWTC 7 cut(s) 550, 848, 1203, 1894, 2214, 2305, 2375
PflMI CCANNNNNTGG 3 cut(s) 131, 1439, 1492
PkrI GCNGC 6 cut(s) 29, 362, 828, 1742, 2128, 3009
PleI GAGTC 4 cut(s) 143, 2277, 2423, 2711
PpsI GAGTC 4 cut(s) 143, 2277, 2423, 2711
PpuMI RGGWCCY 2 cut(s) 329, 689
PscI ACATGT 1 cut(s) 390
PshAI GACNNNNGTC 1 cut(s) 2031
Psp124BI GAGCTC 2 cut(s) 67, 74
Psp5II RGGWCCY 2 cut(s) 329, 689
Psp6I CCWGG 6 cut(s) 891, 1198, 1438, 1596, 1683, 3001
PspEI GGTNACC 1 cut(s) 992
PspFI CCCAGC 1 cut(s) 2133
PspGI CCWGG 6 cut(s) 891, 1198, 1438, 1596, 1683, 3001
PspN4I GGNNCC 2 cut(s) 2240, 3000
PspPI GGNCC 8 cut(s) 329, 689, 1235, 1292, 1681, 1766, 1982, 2066
PspPPI RGGWCCY 2 cut(s) 329, 689
PstI CTGCAG 1 cut(s) 2128
PstNI CAGNNNCTG 2 cut(s) 1439, 2930
PsuI RGATCY 8 cut(s) 121, 806, 1033, 1142, 1435, 1461, 1960, 2998
RsaI GTAC 8 cut(s) 1746, 1759, 1887, 2083, 2394, 2503, 2911, 2983
RsaNI GTAC 8 cut(s) 1745, 1758, 1886, 2082, 2393, 2502, 2910, 2982
RseI CAYNNNNRTG 3 cut(s) 2400, 2685, 2933
SacI GAGCTC 2 cut(s) 67, 74
SatI GCNGC 6 cut(s) 28, 361, 827, 1741, 2127, 3008
Sau96I GGNCC 8 cut(s) 329, 689, 1235, 1292, 1681, 1766, 1982, 2066
ScaI AGTACT 3 cut(s) 1887, 2503, 2911
SchI GAGTC 4 cut(s) 143, 2278, 2423, 2711
ScrFI CCNGG 7 cut(s) 593, 893, 1200, 1440, 1598, 1685, 3003
SduI GDGCHC 3 cut(s) 67, 74, 2783
SfaNI GCATC 4 cut(s) 14, 350, 2138, 3019
SfcI CTRYAG 2 cut(s) 2019, 2124
SfuI TTCGAA 1 cut(s) 2303
SgrAI CRCCGGYG 1 cut(s) 2974
SinI GGWCC 7 cut(s) 329, 689, 1235, 1292, 1766, 1982, 2066
SmiMI CAYNNNNRTG 3 cut(s) 2400, 2685, 2933
SmlI CTYRAG 1 cut(s) 725
SmoI CTYRAG 1 cut(s) 725
SpeI ACTAGT 2 cut(s) 1832, 2710
SsiI CCGC 5 cut(s) 27, 210, 543, 929, 2513
SspI AATATT 4 cut(s) 371, 841, 865, 2419
SstI GAGCTC 2 cut(s) 67, 74
StyD4I CCNGG 7 cut(s) 591, 891, 1198, 1438, 1596, 1683, 3001
StyI CCWWGG 3 cut(s) 660, 1288, 1486
TaaI ACNGT 5 cut(s) 200, 708, 1402, 1519, 2860
TaiI ACGT 3 cut(s) 563, 1250, 1579
TaqI TCGA 6 cut(s) 19, 156, 303, 2303, 2427, 2667
TatI WGTACW 5 cut(s) 1885, 2392, 2501, 2909, 2981
TauI GCSGC 1 cut(s) 30
TfiI GAWTC 7 cut(s) 550, 848, 1203, 1894, 2214, 2305, 2375
TscAI CASTG 7 cut(s) 136, 711, 769, 1792, 2227, 2620, 2935
TseFI GTSAC 2 cut(s) 311, 1309
TseI GCWGC 5 cut(s) 360, 826, 1740, 2126, 3007
Tsp45I GTSAC 2 cut(s) 311, 1309
TspDTI ATGAA 7 cut(s) 476, 812, 1461, 1700, 1971, 2703, 3029
TspGWI ACGGA 1 cut(s) 3041
TspRI CASTG 7 cut(s) 136, 711, 769, 1792, 2227, 2620, 2935
Van91I CCANNNNNTGG 3 cut(s) 131, 1439, 1492
VneI GTGCAC 1 cut(s) 2779
VpaK11BI GGWCC 7 cut(s) 329, 689, 1235, 1292, 1766, 1982, 2066
XbaI TCTAGA 1 cut(s) 2602
XceI RCATGY 1 cut(s) 394
XcmI CCANNNNNNNNNTGG 1 cut(s) 1214
XmaJI CCTAGG 1 cut(s) 1288
XmiI GTMKAC 2 cut(s) 478, 1397
XmnI GAANNNNTTC 1 cut(s) 2833
ZrmI AGTACT 3 cut(s) 1887, 2503, 2911
Zsp2I ATGCAT 1 cut(s) 2284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.