RLG00000032392

Di-glucose binding within endoplasmic reticulum

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000088
Physical Location & Seq
Forward (+)
14329384 .. 14363003
33620 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032392

Sequence Viewer

Length: 2808 bp
ATGGCTCAAAATGAAACTAATGCTACTACGGACCCATCTAAAGTGTCGGCTTTGAACTCGATCTTCCAACAGTGGGGAGCGCAGTCAGTGGCTCTGTGGAATATCAGTGGAGAGCCATGCAGTGGATCAGCCATCAATGGAACTGAATTTGAAAGTGGCCCAAATAACCCGGCCATTATATGTGATTGTACCTTTGACAGTGGTACCACCTGCCATATAACCCAACTGAGAGTTTATGCTCTAGACAAAACAGGAGGGTTTCCAGAAGAGCTTCTGGCTTTGAATATCTTACCTTTCTGTATGAGTTCTAATTTTCATTTCCTTCTTTGGTTTTTAATTTGTTTGTCAATTGCCCACAATGCATTCTCTGGGCCGATCCCCAAGGAACTCGGAAATCTGAAGGACCTAAACATGCTGTCGATTGCCCAGAATAACTTCTATGGACCCATCCCCAAGGAGCTTGGAAACCTTAAGAAACTAAATCTGCTTTACCTAGACAGCTCTGGACTTGGTGGTGAAATTCCTAAAACATTAGCTAAACTTCAAAAAATGCGAATCCTATGGGCATCAGACAGTCCTTTCTCAGGAAAGATACCGGATTTCATAGGGAATTGGACAAAGCTAACCACCTTACGAATCAGTGATCTCTATAATGTGAGCTCCTCTCTTGACTTCATAAGGAATTTGAAGAACTTGACTGATTTAGTTCTCCGAAATGCATTAGTCACTGGCCGCATCCCTTCTGATATTAGAGAATATCAAAATTTAAATATACTGAATCTGGGTTTCAACAATATAACAGGTCAACTCCCAAGTGACTTGTTCAACATGAGTTCTCTCGTATCCTTATTTCTTGGAAATAATAGTTTGTCTGGAGCTCTGCCTAGCCAAAAGAGTGATAGTCTTCAAACCATAGATTTATCTTATAACTATTTATCAGGAAGTCTTCCCCCATGGGTAACCACAGTATCGCAACTGAACTTGGTGGCCAACAACTTCACTTTTAATGAAAGTTCAGAAATAATGCTTCCTGGATTGAATTGCCTTCAAAGAAATTTTCCATGCAATAGAAATCCCCCGCGCTATGCAAACTTCTCAATCAACTGTGGAGGACCAGACATGAGAGGAAGTGATGGCATATTATATGAGGCAGAAAACTCAACTCTTGGCCCAGCAACATATAATGTAACCAGTACAGAGAAATGGGCTGTTAGCAATGTGGGTTTGTTTGCTGAGAGGAAGAATCCAAGCTATTTGCTAACCTCCCTGGAACAGGCTACTGGGTCAGATGTGACCCCAGAGCTTTTCCAGACTTCAAGATTATCTCCAGGATCACTGAGATACTATGGTTTGGGTCTCGAGAATGGGCCTTATACTGTAAAATTACAATTTGCAGAAATGGTTTTTGATGATCGTGCATCACAAACTTGGAAAAGTTTAGGAAGGCGAGTATTTGATATCTATATTCAGGGGCATCTAGTAGAGAAGGACTTTGACATATCCAAGGAGGCAGGTGGTGTTTTAAGAGTAGCAGTGAGAAACTTCAATGTAAATGTGTCGGACAATTATGTTGAAATTCATTTGTTCTGGGCTGGTAAGGGGACCTGCTGCACACCTAACCAAGGTGATTACGGTCCACTAATAGCAACGGTCAATGCCGTTTCAGATTTTAAACCAACTGTTTCTGGGATTCCACCAACTACTCCAGGAAAGAAGAGCATGACAGGGGAGATTGTTGGTGTTGCAGTCTCTGTTGGAGTTGTGAGCTTGCTATTAATATTTGCAGTTTTATATTTGAGGAGAAAAATACCAGACAAGGATGACGATGAAGCGAGGTTAGTGGCTGTGAAGCAACTTTCAGTAGCATCTCACCAAGGGAAGAGTCAATTTGCAGCAGAAATTGCTGCCATATCTGCAGTGCAACATCGCAATCTGGTGAAACTGTATGGATGCTGTATCGAAGGAAACCAGCGAATTTTGGTTTATGAATATCTTGAAAACAAGAGCCTTGATCAGGCACTTTTTGGAAACAACTTGCATCTTGATTGGCATACCAGATTCAATATCTTGTTGGGAACAGCAAGAGGACTTGCCTATCTTCATGAGGAGTCGAGGCCAAGGATTGTACATAGAGATGTCAAGGCCAGTAATATTTTGCTTGATGCAGACCTCTGCCCAAAAATATCAGATTTTGGACTGGCAAAGCTTTATGATGATGAGAAAACCCATATCAGCACCCGGGTTGCAGGGACAATAGGCTATTTGGCACCGGAGTATGCAATGCGGGGACATCTGACTGAAAAGGCTGATGTATTCGGTTTTGGAGTTGTCATTTTGGAGACTCTTAGTGGGAGACCAAACACTGACAATGACTTGGATGCCGGAGAGATTTATCTCCTTGATTGGGCATGGACTCTGCATGAAAAAAATGAGAGTTTGAGGCTTGTGGATCCGAGACTGACCGATTTTGATGAAGAAGATGCAAATAGATTGATAAGAGCAGGTTTCCTGTGTACTCAGGCATCCCCATTGATGCGGCCATCTATGTCTCGTGTAGTGGCCATGCTTGCTGGAGATATTGAAGTAGGTAATGTCATGGCAAAGCCTAGTTATTTGACAGACTGGGATTTCAAAGATGTAACAGGTAGCTTCTTGATTGATGGTGATACCCCCTCAACTGAGATGACTCAACCCAACAATCAGTCTACTGGCATCACAACCGGTGCTAACACCGGAATTGACCTGATGCAGTCTCCTGTGAATCCTAATCATTCATTGCTCACTGGGATTATAGGAGATGGAAGGTGA

Protein Analysis

936

Amino Acids

102.67

Weight (kDa)

5.32

Isoelectric Point (pI)

34.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 128 - 235 1.3e-11 Leucine-rich repeat region
Malectin PF11721 365 - 549 4.6e-40 Malectin domain
PK_Tyr_Ser-Thr PF07714 606 - 853 2.4e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 608 - 791 6.5e-38 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 927
AarI CACCTGC 2 cut(s) 218, 1502
Acc36I ACCTGC 4 cut(s) 218, 1502, 1613, 2492
Acc65I GGTACC 1 cut(s) 203
AccB1I GGYRCC 2 cut(s) 203, 2266
AccB7I CCANNNNNTGG 1 cut(s) 122
AccI GTMKAC 1 cut(s) 2705
AccII CGCG 1 cut(s) 1081
AciI CCGC 4 cut(s) 733, 1079, 2284, 2536
AclWI GGATC 5 cut(s) 133, 370, 1339, 2444, 2457
AcoI YGGCCR 5 cut(s) 171, 730, 987, 2537, 2559
AcsI RAATTY 7 cut(s) 146, 519, 682, 763, 1054, 1575, 1974
AcuI CTGAAG 1 cut(s) 419
AfaI GTAC 5 cut(s) 190, 205, 1195, 2127, 2515
AfiI CCNNNNNNNGG 7 cut(s) 73, 122, 584, 1273, 1622, 2014, 2404
AflII CTTAAG 1 cut(s) 470
AgeI ACCGGT 1 cut(s) 2720
AjnI CCWGG 4 cut(s) 1030, 1266, 1327, 1705
Alw21I GWGCWC 2 cut(s) 662, 880
Alw26I GTCTC 7 cut(s) 1361, 1753, 2333, 2347, 2449, 2553, 2757
AlwI GGATC 5 cut(s) 133, 370, 1339, 2444, 2457
AlwNI CAGNNNCTG 1 cut(s) 1751
Ama87I CYCGRG 2 cut(s) 1358, 2238
ApeKI GCWGC 3 cut(s) 1608, 1892, 1904
ApoI RAATTY 7 cut(s) 146, 519, 682, 763, 1054, 1575, 1974
AseI ATTAAT 1 cut(s) 1775
AsiGI ACCGGT 1 cut(s) 2720
Asp700I GAANNNNTTC 2 cut(s) 270, 434
Asp718I GGTACC 1 cut(s) 203
AspLEI GCGC 2 cut(s) 82, 1083
AsuC2I CCSGG 3 cut(s) 170, 2239, 2240
AsuHPI GGTGA 5 cut(s) 527, 1637, 1862, 1948, 2675
AvaI CYCGRG 2 cut(s) 1358, 2238
AvaII GGWCC 6 cut(s) 31, 403, 443, 1112, 1602, 1634
BaeI ACNNNNGTAYC 6 cut(s) 187, 220, 951, 984, 1333, 1366
BalI TGGCCA 2 cut(s) 989, 2561
BamHI GGATCC 1 cut(s) 2449
BanI GGYRCC 2 cut(s) 203, 2266
BanII GRGCYC 2 cut(s) 662, 880
BauI CACGAG 1 cut(s) 2550
BbsI GAAGAC 2 cut(s) 896, 938
Bbv12I GWGCWC 2 cut(s) 662, 880
BbvI GCAGC 3 cut(s) 1595, 1891, 1904
BccI CCATC 7 cut(s) 43, 140, 455, 1127, 2548, 2654, 2792
BceAI ACGGC 1 cut(s) 1643
BciT130I CCWGG 4 cut(s) 1032, 1268, 1329, 1707
BciVI GTATCC 1 cut(s) 853
BclI TGATCA 1 cut(s) 2011
BcnI CCSGG 3 cut(s) 170, 2239, 2240
BcoDI GTCTC 7 cut(s) 1361, 1753, 2333, 2347, 2449, 2553, 2757
BfaI CTAG 5 cut(s) 242, 494, 885, 1478, 2607
BfmI CTRYAG 1 cut(s) 1914
BfrI CTTAAG 1 cut(s) 470
BfuAI ACCTGC 4 cut(s) 218, 1502, 1613, 2492
BfuI GTATCC 1 cut(s) 853
BisI GCNGC 5 cut(s) 733, 1609, 1893, 1905, 2537
BlsI GCNGC 5 cut(s) 734, 1610, 1894, 1906, 2538
Bme1390I CCNGG 7 cut(s) 170, 1032, 1268, 1329, 1707, 2239, 2240
Bme18I GGWCC 6 cut(s) 31, 403, 443, 1112, 1602, 1634
BmeT110I CYCGRG 2 cut(s) 1358, 2238
BmiI GGNNCC 6 cut(s) 33, 205, 445, 1603, 2268, 2451
BmrFI CCNGG 7 cut(s) 170, 1032, 1268, 1329, 1707, 2239, 2240
BmrI ACTGGG 3 cut(s) 1290, 2632, 2793
BmuI ACTGGG 3 cut(s) 1290, 2632, 2793
BpiI GAAGAC 2 cut(s) 896, 938
BplI GAGNNNNNCTC 2 cut(s) 649, 681
BpmI CTGGAG 4 cut(s) 894, 1311, 1689, 2592
BpuMI CCSGG 3 cut(s) 170, 2239, 2240
BsaI GGTCTC 2 cut(s) 1361, 2347
BsaJI CCNNGG 9 cut(s) 381, 453, 953, 1266, 1503, 1621, 1873, 2117, 2238
BsaWI WCCGGW 4 cut(s) 595, 2269, 2720, 2732
Bsc4I CCNNNNNNNGG 7 cut(s) 73, 122, 584, 1273, 1622, 2014, 2404
Bse118I RCCGGY 1 cut(s) 2720
Bse1I ACTGG 8 cut(s) 733, 1191, 1285, 2145, 2202, 2627, 2713, 2788
Bse3DI GCAATG 3 cut(s) 1222, 2286, 2774
BseBI CCWGG 4 cut(s) 1032, 1268, 1329, 1707
BseDI CCNNGG 9 cut(s) 381, 453, 953, 1266, 1503, 1621, 1873, 2117, 2238
BseGI GGATG 6 cut(s) 447, 735, 1825, 1955, 2383, 2522
BseLI CCNNNNNNNGG 7 cut(s) 73, 122, 584, 1273, 1622, 2014, 2404
BseMI GCAATG 3 cut(s) 1222, 2286, 2774
BseMII CTCAG 6 cut(s) 218, 597, 1224, 1328, 2531, 2670
BseNI ACTGG 8 cut(s) 733, 1191, 1285, 2145, 2202, 2627, 2713, 2788
BseRI GAGGAG 3 cut(s) 652, 1813, 2120
BseXI GCAGC 3 cut(s) 1595, 1891, 1904
BseYI CCCAGC 1 cut(s) 1171
BsgI GTGCAG 1 cut(s) 1594
Bsh1236I CGCG 1 cut(s) 1081
BshNI GGYRCC 2 cut(s) 203, 2266
BshTI ACCGGT 1 cut(s) 2720
BsiHKAI GWGCWC 2 cut(s) 662, 880
BsiHKCI CYCGRG 2 cut(s) 1358, 2238
BsiSI CCGG 7 cut(s) 170, 596, 2239, 2270, 2382, 2721, 2733
BslFI GGGAC 3 cut(s) 1615, 2263, 2301
BslI CCNNNNNNNGG 7 cut(s) 73, 122, 584, 1273, 1622, 2014, 2404
BsmAI GTCTC 7 cut(s) 1361, 1753, 2333, 2347, 2449, 2553, 2757
BsmFI GGGAC 3 cut(s) 1615, 2263, 2301
BsmI GAATGC 1 cut(s) 362
Bso31I GGTCTC 2 cut(s) 1361, 2347
BsoBI CYCGRG 2 cut(s) 1358, 2238
Bsp1286I GDGCHC 2 cut(s) 662, 880
Bsp1407I TGTACA 1 cut(s) 2125
Bsp143I GATC 8 cut(s) 60, 125, 375, 643, 1331, 1411, 2011, 2449
Bsp19I CCATGG 1 cut(s) 953
BspACI CCGC 4 cut(s) 733, 1079, 2284, 2536
BspCNI CTCAG 6 cut(s) 219, 596, 1225, 1329, 2530, 2671
BspFNI CGCG 1 cut(s) 1081
BspHI TCATGA 1 cut(s) 2101
BspLI GGNNCC 6 cut(s) 33, 205, 445, 1603, 2268, 2451
BspMAI CTGCAG 1 cut(s) 1918
BspMI ACCTGC 4 cut(s) 218, 1502, 1613, 2492
BspPI GGATC 5 cut(s) 133, 370, 1339, 2444, 2457
BspQI GCTCTTC 2 cut(s) 261, 1709
BspT107I GGYRCC 2 cut(s) 203, 2266
BspTI CTTAAG 1 cut(s) 470
BspTNI GGTCTC 2 cut(s) 1361, 2347
BsrDI GCAATG 3 cut(s) 1222, 2286, 2774
BsrFI RCCGGY 1 cut(s) 2720
BsrGI TGTACA 1 cut(s) 2125
BsrI ACTGG 8 cut(s) 733, 1191, 1285, 2145, 2202, 2627, 2713, 2788
BssAI RCCGGY 1 cut(s) 2720
BssECI CCNNGG 9 cut(s) 381, 453, 953, 1266, 1503, 1621, 1873, 2117, 2238
BssMI GATC 8 cut(s) 60, 125, 375, 643, 1331, 1411, 2011, 2449
BssSI CACGAG 1 cut(s) 2550
BssT1I CCWWGG 7 cut(s) 381, 453, 953, 1503, 1621, 1873, 2117
Bst2BI CACGAG 1 cut(s) 2550
Bst2UI CCWGG 4 cut(s) 1032, 1268, 1329, 1707
Bst6I CTCTTC 3 cut(s) 261, 1709, 1874
BstAFI CTTAAG 1 cut(s) 470
BstAPI GCANNNNNTGC 1 cut(s) 1901
BstAUI TGTACA 1 cut(s) 2125
BstC8I GCNNGC 2 cut(s) 1769, 2568
BstDEI CTNAG 7 cut(s) 227, 583, 1233, 1337, 2345, 2517, 2679
BstDSI CCRYGG 1 cut(s) 953
BstEII GGTNACC 1 cut(s) 958
BstENI CCTNNNNNAGG 4 cut(s) 582, 1271, 1620, 2012
BstF5I GGATG 6 cut(s) 447, 735, 1825, 1955, 2383, 2522
BstFNI CGCG 1 cut(s) 1081
BstHHI GCGC 2 cut(s) 82, 1083
BstKTI GATC 8 cut(s) 63, 128, 378, 646, 1334, 1414, 2014, 2452
BstMAI GTCTC 7 cut(s) 1361, 1753, 2333, 2347, 2449, 2553, 2757
BstMBI GATC 8 cut(s) 60, 125, 375, 643, 1331, 1411, 2011, 2449
BstMWI GCNNNNNNNGC 4 cut(s) 359, 1901, 1913, 2567
BstNI CCWGG 4 cut(s) 1032, 1268, 1329, 1707
BstNSI RCATGY 1 cut(s) 415
BstPI GGTNACC 1 cut(s) 958
BstSCI CCNGG 7 cut(s) 168, 1030, 1266, 1327, 1705, 2237, 2238
BstSFI CTRYAG 1 cut(s) 1914
BstUI CGCG 1 cut(s) 1081
BstV1I GCAGC 3 cut(s) 1595, 1891, 1904
BstV2I GAAGAC 2 cut(s) 896, 938
BstX2I RGATCY 1 cut(s) 2449
BstYI RGATCY 1 cut(s) 2449
BsuI GTATCC 1 cut(s) 853
BtgI CCRYGG 1 cut(s) 953
BtgZI GCGATG 1 cut(s) 1910
BtsCI GGATG 6 cut(s) 447, 735, 1825, 1955, 2383, 2522
BtsI GCAGTG 3 cut(s) 127, 1539, 1923
BveI ACCTGC 4 cut(s) 218, 1502, 1613, 2492
Cac8I GCNNGC 2 cut(s) 1769, 2568
CaiI CAGNNNCTG 1 cut(s) 1751
CciI TCATGA 1 cut(s) 2101
CfoI GCGC 2 cut(s) 82, 1083
Cfr10I RCCGGY 1 cut(s) 2720
Cfr9I CCCGGG 1 cut(s) 2238
Csp6I GTAC 5 cut(s) 189, 204, 1194, 2126, 2514
CspAI ACCGGT 1 cut(s) 2720
CviQI GTAC 5 cut(s) 189, 204, 1194, 2126, 2514
DdeI CTNAG 7 cut(s) 227, 583, 1233, 1337, 2345, 2517, 2679
DpnI GATC 8 cut(s) 62, 127, 377, 645, 1333, 1413, 2013, 2451
DpnII GATC 8 cut(s) 60, 125, 375, 643, 1331, 1411, 2011, 2449
DraI TTTAAA 2 cut(s) 768, 1672
EaeI YGGCCR 5 cut(s) 171, 730, 987, 2537, 2559
Eam1104I CTCTTC 3 cut(s) 261, 1709, 1874
EarI CTCTTC 3 cut(s) 261, 1709, 1874
Ecl136II GAGCTC 2 cut(s) 660, 878
Eco130I CCWWGG 7 cut(s) 381, 453, 953, 1503, 1621, 1873, 2117
Eco24I GRGCYC 2 cut(s) 662, 880
Eco31I GGTCTC 2 cut(s) 1361, 2347
Eco32I GATATC 1 cut(s) 1459
Eco47I GGWCC 6 cut(s) 31, 403, 443, 1112, 1602, 1634
Eco53kI GAGCTC 2 cut(s) 660, 878
Eco57I CTGAAG 1 cut(s) 419
Eco88I CYCGRG 2 cut(s) 1358, 2238
Eco91I GGTNACC 1 cut(s) 958
EcoICRI GAGCTC 2 cut(s) 660, 878
EcoNI CCTNNNNNAGG 4 cut(s) 582, 1271, 1620, 2012
EcoO109I RGGNCCY 2 cut(s) 403, 1602
EcoO65I GGTNACC 1 cut(s) 958
EcoRII CCWGG 4 cut(s) 1030, 1266, 1327, 1705
EcoRV GATATC 1 cut(s) 1459
EcoT14I CCWWGG 7 cut(s) 381, 453, 953, 1503, 1621, 1873, 2117
EcoT22I ATGCAT 2 cut(s) 364, 721
EcoT38I GRGCYC 2 cut(s) 662, 880
ErhI CCWWGG 7 cut(s) 381, 453, 953, 1503, 1621, 1873, 2117
FaqI GGGAC 3 cut(s) 1615, 2263, 2301
FauI CCCGC 2 cut(s) 1086, 2277
FbaI TGATCA 1 cut(s) 2011
FblI GTMKAC 1 cut(s) 2705
Fnu4HI GCNGC 5 cut(s) 733, 1609, 1893, 1905, 2537
FokI GGATG 6 cut(s) 434, 722, 1832, 1962, 2390, 2509
FriOI GRGCYC 2 cut(s) 662, 880
Fsp4HI GCNGC 5 cut(s) 733, 1609, 1893, 1905, 2537
FspBI CTAG 5 cut(s) 242, 494, 885, 1478, 2607
GlaI GCGC 2 cut(s) 81, 1082
GluI GCNGC 5 cut(s) 733, 1609, 1893, 1905, 2537
GsaI CCCAGC 1 cut(s) 1175
GsuI CTGGAG 4 cut(s) 894, 1311, 1689, 2592
HapII CCGG 7 cut(s) 170, 596, 2239, 2270, 2382, 2721, 2733
HhaI GCGC 2 cut(s) 82, 1083
Hin6I GCGC 2 cut(s) 80, 1081
HinP1I GCGC 2 cut(s) 80, 1081
HincII GTYRAC 1 cut(s) 806
HindII GTYRAC 1 cut(s) 806
HindIII AAGCTT 1 cut(s) 2204
HpaII CCGG 7 cut(s) 170, 596, 2239, 2270, 2382, 2721, 2733
HphI GGTGA 5 cut(s) 527, 1637, 1862, 1948, 2675
Hpy166II GTNNAC 4 cut(s) 806, 1637, 2514, 2706
Hpy8I GTNNAC 4 cut(s) 806, 1637, 2514, 2706
HpyAV CCTTC 8 cut(s) 332, 394, 750, 1055, 1437, 1480, 1955, 2796
HpyF10VI GCNNNNNNNGC 4 cut(s) 359, 1901, 1913, 2567
HpyF3I CTNAG 7 cut(s) 227, 583, 1233, 1337, 2345, 2517, 2679
HspAI GCGC 2 cut(s) 80, 1081
KpnI GGTACC 1 cut(s) 207
Ksp22I TGATCA 1 cut(s) 2011
Kzo9I GATC 8 cut(s) 60, 125, 375, 643, 1331, 1411, 2011, 2449
LguI GCTCTTC 2 cut(s) 261, 1709
LmnI GCTCC 4 cut(s) 77, 457, 665, 875
Lsp1109I GCAGC 3 cut(s) 1595, 1891, 1904
MaeI CTAG 5 cut(s) 242, 494, 885, 1478, 2607
MaeIII GTNAC 6 cut(s) 724, 815, 958, 1186, 1291, 2638
MalI GATC 8 cut(s) 62, 127, 377, 645, 1333, 1413, 2013, 2451
MboI GATC 8 cut(s) 60, 125, 375, 643, 1331, 1411, 2011, 2449
MfeI CAATTG 1 cut(s) 348
MflI RGATCY 1 cut(s) 2449
MhlI GDGCHC 2 cut(s) 662, 880
MlsI TGGCCA 2 cut(s) 989, 2561
MluNI TGGCCA 2 cut(s) 989, 2561
MlyI GAGTC 5 cut(s) 1891, 2117, 2335, 2407, 2680
MmeI TCCRAC 3 cut(s) 91, 1539, 1735
Mox20I TGGCCA 2 cut(s) 989, 2561
Mph1103I ATGCAT 2 cut(s) 364, 721
MroXI GAANNNNTTC 2 cut(s) 270, 434
MscI TGGCCA 2 cut(s) 989, 2561
MseI TTAA 7 cut(s) 335, 471, 767, 1005, 1523, 1671, 1775
MslI CAYNNNNRTG 1 cut(s) 2133
Msp20I TGGCCA 2 cut(s) 989, 2561
MspCI CTTAAG 1 cut(s) 470
MspI CCGG 7 cut(s) 170, 596, 2239, 2270, 2382, 2721, 2733
MspR9I CCNGG 7 cut(s) 170, 1032, 1268, 1329, 1707, 2239, 2240
MunI CAATTG 1 cut(s) 348
Mva1269I GAATGC 1 cut(s) 362
MvaI CCWGG 4 cut(s) 1032, 1268, 1329, 1707
MvnI CGCG 1 cut(s) 1081
MwoI GCNNNNNNNGC 4 cut(s) 359, 1901, 1913, 2567
NciI CCSGG 3 cut(s) 170, 2239, 2240
NcoI CCATGG 1 cut(s) 953
NdeII GATC 8 cut(s) 60, 125, 375, 643, 1331, 1411, 2011, 2449
NlaIV GGNNCC 6 cut(s) 33, 205, 445, 1603, 2268, 2451
NmuCI GTSAC 3 cut(s) 724, 815, 1291
NsiI ATGCAT 2 cut(s) 364, 721
NspI RCATGY 1 cut(s) 415
PaeR7I CTCGAG 1 cut(s) 1358
PagI TCATGA 1 cut(s) 2101
PaqCI CACCTGC 2 cut(s) 218, 1502
PciSI GCTCTTC 2 cut(s) 261, 1709
PctI GAATGC 1 cut(s) 362
PdmI GAANNNNTTC 2 cut(s) 270, 434
PfeI GAWTC 7 cut(s) 555, 636, 778, 1243, 1690, 2058, 2761
PflMI CCANNNNNTGG 1 cut(s) 122
PfoI TCCNGGA 3 cut(s) 1030, 1327, 1705
PinAI ACCGGT 1 cut(s) 2720
PkrI GCNGC 5 cut(s) 734, 1610, 1894, 1906, 2538
PleI GAGTC 5 cut(s) 1890, 2116, 2335, 2407, 2680
PpsI GAGTC 5 cut(s) 1890, 2116, 2335, 2407, 2680
PpuMI RGGWCCY 2 cut(s) 403, 1602
PshBI ATTAAT 1 cut(s) 1775
PsiI TTATAA 1 cut(s) 927
Psp124BI GAGCTC 2 cut(s) 662, 880
Psp5II RGGWCCY 2 cut(s) 403, 1602
Psp6I CCWGG 4 cut(s) 1030, 1266, 1327, 1705
PspEI GGTNACC 1 cut(s) 958
PspFI CCCAGC 1 cut(s) 1171
PspGI CCWGG 4 cut(s) 1030, 1266, 1327, 1705
PspN4I GGNNCC 6 cut(s) 33, 205, 445, 1603, 2268, 2451
PspPPI RGGWCCY 2 cut(s) 403, 1602
PstI CTGCAG 1 cut(s) 1918
PstNI CAGNNNCTG 1 cut(s) 1751
PsuI RGATCY 1 cut(s) 2449
RsaI GTAC 5 cut(s) 190, 205, 1195, 2127, 2515
RsaNI GTAC 5 cut(s) 189, 204, 1194, 2126, 2514
RseI CAYNNNNRTG 1 cut(s) 2133
SacI GAGCTC 2 cut(s) 662, 880
SapI GCTCTTC 2 cut(s) 261, 1709
SaqAI TTAA 7 cut(s) 335, 471, 767, 1005, 1523, 1671, 1775
SatI GCNGC 5 cut(s) 733, 1609, 1893, 1905, 2537
Sau3AI GATC 8 cut(s) 60, 125, 375, 643, 1331, 1411, 2011, 2449
SchI GAGTC 5 cut(s) 1891, 2117, 2335, 2407, 2680
ScrFI CCNGG 7 cut(s) 170, 1032, 1268, 1329, 1707, 2239, 2240
SduI GDGCHC 2 cut(s) 662, 880
SfcI CTRYAG 1 cut(s) 1914
Sfr274I CTCGAG 1 cut(s) 1358
SinI GGWCC 6 cut(s) 31, 403, 443, 1112, 1602, 1634
SlaI CTCGAG 1 cut(s) 1358
SmaI CCCGGG 1 cut(s) 2240
SmiI ATTTAAAT 1 cut(s) 768
SmiMI CAYNNNNRTG 1 cut(s) 2133
SmlI CTYRAG 2 cut(s) 470, 1358
SmoI CTYRAG 2 cut(s) 470, 1358
SsiI CCGC 4 cut(s) 733, 1079, 2284, 2536
SspI AATATT 2 cut(s) 1779, 2152
SspMI CTAG 5 cut(s) 242, 494, 885, 1478, 2607
SstI GAGCTC 2 cut(s) 662, 880
StyD4I CCNGG 7 cut(s) 168, 1030, 1266, 1327, 1705, 2237, 2238
StyI CCWWGG 7 cut(s) 381, 453, 953, 1503, 1621, 1873, 2117
SwaI ATTTAAAT 1 cut(s) 768
TaqI TCGA 5 cut(s) 59, 419, 1359, 1959, 2111
TaqII GACCGA 1 cut(s) 2477
TatI WGTACW 3 cut(s) 1193, 2125, 2513
TauI GCSGC 2 cut(s) 735, 2539
TfiI GAWTC 7 cut(s) 555, 636, 778, 1243, 1690, 2058, 2761
Tru1I TTAA 7 cut(s) 335, 471, 767, 1005, 1523, 1671, 1775
Tru9I TTAA 7 cut(s) 335, 471, 767, 1005, 1523, 1671, 1775
TseFI GTSAC 3 cut(s) 724, 815, 1291
TseI GCWGC 3 cut(s) 1608, 1892, 1904
Tsp45I GTSAC 3 cut(s) 724, 815, 1291
TspGWI ACGGA 1 cut(s) 44
TspMI CCCGGG 1 cut(s) 2238
Van91I CCANNNNNTGG 1 cut(s) 122
Vha464I CTTAAG 1 cut(s) 470
VpaK11BI GGWCC 6 cut(s) 31, 403, 443, 1112, 1602, 1634
VspI ATTAAT 1 cut(s) 1775
XagI CCTNNNNNAGG 4 cut(s) 582, 1271, 1620, 2012
XapI RAATTY 7 cut(s) 146, 519, 682, 763, 1054, 1575, 1974
XbaI TCTAGA 1 cut(s) 241
XceI RCATGY 1 cut(s) 415
XhoI CTCGAG 1 cut(s) 1358
XmaI CCCGGG 1 cut(s) 2238
XmiI GTMKAC 1 cut(s) 2705
XmnI GAANNNNTTC 2 cut(s) 270, 434
XspI CTAG 5 cut(s) 242, 494, 885, 1478, 2607
Zsp2I ATGCAT 2 cut(s) 364, 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.