Rroxscaffold_2G00120060
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000088
Physical Location & Seq
Reverse (-)
52132199 .. 52141635
9437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00120060.1

Sequence Viewer

Length: 2652 bp
ATGTTATCAGGCCAGCTACCAAAGGAACTCGGAAATCTCTCTAATTTGACATTCTTGGCTATTGGGACTATGAACCTTTCCGGTGGTCTACCACATGAGCTAGGGAATTTATTAAATTTACAACAGATTTACTTGGATAGTTCTGGAGTTAGCGGTGAGATTCCACCGACGTTTGCTAATCTACGAAACTTGACCATATTCCGGGTGTCAGACACAGAACTCTCTGGCAGGATACCTGACTTCGTAGGATATTGGTCAAAGCTTACTTCCTTGGTATTTCAAGGGAACTCTTTTGAAGGTCCTATACCAGCTACACTGTCCAATCTGACTTCTCTTGAGGAGCTGAGAATTAGTGATATAATATCTACTGCCAGTGGGAACTCTTCTCTTGGATTTATTAAGGATATGAAGTCAAGATATCTAAAAGTCCTAGTGCTGCGAAATAACAATATTTCTGATTCAATTCCTTCTAATATTGGAGAGTTGCAAAGTTTGTATCACCTGGATTTAAGCTTCAACAATTTAGCAGGAGTAATACCGCCTTCCCTGTTTAATTTGCCTTCGCTCAAAGCCCTGTTTCTTGGAAACAACAAGTTGAGTGGTAACCTTCCTGAAAATAAAAGCTCGTCTCTTGTCAATGTAGATCTGTCTTACAATAATCTAGTAGGGTCAAGCTTTCCTTCTTGGGTGAACGAAGACTTACAGCTTAATTTAGTTGCCAACAACTTCAGCACAGAAAATTCAAACGGCAGATCTTTGCCTTCAGGGTTGAAATGCCTCCAAAGGAACTTTCCTTGCAATGTTGACCCTGGAATCCATCCTTACATTCGGATTAAATGTGGTGGTCCTCAGTTCACGTCTTCCAATGGGATTGTGTATGATCAGGAAAATGAGACCCTTGGTCCAGCTACATATTTTGTGACTGGCACAAGCAGATGGGGAGTTAGCAATGTTGGTATTAAAACAGAGTATCAGGATGGATATATCACGGGAAAAATTCCTGAGTATACAGTTTCCTCGTCATCTCAATTCACGGATACTTCAGATCCTGAGCTATTCCGGACTGCAAGGATCTCTGCTTCATCACTTAGATACTATGGCTTGGGGCTGCAGAATGGTGTCTACACAGTCAAACTTCAGTTTGCAGAACAAAAAATCCTAGATACTCCAAAAAATCTTGGGAGACGTGTATTTGATATATATATCCAGGGAATTCTAGTTTCGAAGGATTTTGATATACGAAAGGAAGCAGGTGGGAAATCTTTTCAAGCCGTTCAGAAGGAATATAAGGCCCAGGTTTCACAGAACTACCTTGAAATTCATCTCTTTTGGGCTGGAAAGGGGACTTGCTGCGTACCTGAGGTTGGTACTTATGGACCTGTTATTTCAGCCATCAGTGCTACACCAGATTTCCTACCTACTATCGTTCCGAACCATCCAACTAGTAAGAAGAATAGGACGGGGCTGATTGTGGGGATTGTTGTTGGTGGTGGAGTACTTTTGATTCTATTGTTGTTGGTTTTCTGTATAGTTCAAAGAAGTAAAAGGATTAACACCAGTAAAAATGAAGATCTGTTGGGAATAGATATTGGACCACTCACTTTCAGTTTTTCAGAGCTAAAGACAGCTACAGATGAGTTTAGTCCAGCGAATAAGATTGGAGAGGGAGGATTTGGACCTGTCTATAAAGGTACTCTTAATGATGGAAGAGTAATTGCCGTGAAGCAATTGTCTGCAGCATCCCAGCAAGGAAAAAATGAGTTTGTAGCTGAAATTGCCACCATATCTGCCGTGCGACACAATAACCTAGTGAATTTGCAGGGATTCTGCACTGAGGGAGTTAAACGGCTCCTTGTCTATGAGTATCTGGAAAACAAGAGTCTTGATCGTGCATTATTTGCAGGACAAAGAAGTTTGAATCTTGATTGGTCATCCAGATTTGATATATGCTTGGGCGTGGCTAAAGGTTTGACTTATCTCCATGAGGAATCAAGGGTTCGGATTGTACACAGAGATGTGAAGGCCAGTAATATTCTGCTCGACTCTAATCTCATCCCCAAAATATCAGATTTCGGTTTGGCCAGGCTTTATGATGATAAAAAGACCCACATCAGTACTCGTATTGCGGGAACAATTGGGTATCTTGCACCGGAATATGCCATGCGCGGACACCTGACACAGAAGACTGATGTGTTTGCCTTTGGTGTTCTTGCTCTAGAAATTGTCAGTGGTAGGCCAAATTCTGACCGGAGCTTGGAAGGCGAGATGATTTATCTTCTCGAATGGGCTTGGCACTTGCATGAAAACGAACGCGAAGTTGAACTAGTGGACTCTAGGTTATCAGAATTCAATGAGGAAGAGGCAAGACGAATAACCAAGATAGGGCTTTTGTGCACTCAAACATCACCAATGCAACGACCACCAATGTCGCGTGTGGTAGGAATGCTTTCAGGAGATATTGAAGTAATGACAGTAACTTCAAAGCCTGGTTACTTGACAGACTGGGATATGTCAGCAAAGGGAAATGATCCTAGCTTTGACAACTCATCGGCGAGTACAAGCACTTTGGGGGATCCAGGGCAGCTTTCTACAAATGCAACGGATGCCTTGCTTCATAATATTGTTGTTGATACCCAATCCCTACCGACTTAA

Protein Analysis

883

Amino Acids

96.76

Weight (kDa)

6.0

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 8 - 117 5.9e-09 Leucine-rich repeat region
LRR_8 PF13855 140 - 199 3.4e-08 Leucine rich repeat
Malectin PF11721 276 - 465 5.6e-41 Malectin domain
Pkinase PF00069 550 - 749 8.9e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 551 - 814 8.4e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1241
Acc36I ACCTGC 1 cut(s) 1241
AccI GTMKAC 3 cut(s) 88, 1007, 1122
AccII CGCG 3 cut(s) 2166, 2313, 2431
AccIII TCCGGA 1 cut(s) 1059
AciI CCGC 4 cut(s) 153, 539, 2126, 2166
AclWI GGATC 5 cut(s) 1040, 1079, 2522, 2567, 2580
AcoI YGGCCR 1 cut(s) 2079
AcsI RAATTY 9 cut(s) 106, 115, 739, 996, 1212, 1317, 1813, 2239, 2345
AcuI CTGAAG 4 cut(s) 712, 747, 1026, 1121
AfaI GTAC 7 cut(s) 1356, 1369, 1497, 1693, 2007, 2116, 2557
AfiI CCNNNNNNNGG 4 cut(s) 201, 1364, 2254, 2382
AflIII ACRYGT 1 cut(s) 1186
AhdI GACNNNNNGTC 1 cut(s) 900
AhlI ACTAGT 2 cut(s) 1442, 2323
AjiI CACGTC 2 cut(s) 858, 1187
AjnI CCWGG 7 cut(s) 501, 808, 1206, 1293, 2081, 2485, 2575
AjuI GAANNNNNNNTTGG 2 cut(s) 774, 806
AloI GAACNNNNNNTCC 2 cut(s) 1140, 1172
Alw21I GWGCWC 1 cut(s) 2396
Alw26I GTCTC 3 cut(s) 633, 887, 1177
Alw44I GTGCAC 1 cut(s) 2392
AlwI GGATC 5 cut(s) 1040, 1079, 2522, 2567, 2580
AlwNI CAGNNNCTG 1 cut(s) 1049
Aor13HI TCCGGA 1 cut(s) 1059
AoxI GGCC 5 cut(s) 10, 1290, 2022, 2079, 2234
ApaLI GTGCAC 1 cut(s) 2392
ApeKI GCWGC 5 cut(s) 436, 1108, 1350, 1736, 2581
ApoI RAATTY 9 cut(s) 106, 115, 739, 996, 1212, 1317, 1813, 2239, 2345
Asp700I GAANNNNTTC 1 cut(s) 2446
AspLEI GCGC 1 cut(s) 2166
AspS9I GGNCC 7 cut(s) 299, 845, 902, 1291, 1376, 1592, 1676
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 4 cut(s) 167, 491, 700, 2397
AsuII TTCGAA 1 cut(s) 1223
AvaII GGWCC 6 cut(s) 299, 845, 902, 1376, 1592, 1676
AxyI CCTNAGG 1 cut(s) 1359
BaeGI GKGCMC 1 cut(s) 2396
BalI TGGCCA 1 cut(s) 2081
BamHI GGATCC 1 cut(s) 2572
BarI GAAGNNNNNNTAC 2 cut(s) 288, 320
BbsI GAAGAC 3 cut(s) 702, 852, 2189
Bbv12I GWGCWC 1 cut(s) 2396
BbvI GCAGC 5 cut(s) 423, 1095, 1337, 1748, 2593
BccI CCATC 6 cut(s) 825, 930, 971, 1400, 1443, 1697
BceAI ACGGC 5 cut(s) 763, 1256, 1703, 1775, 1862
BciT130I CCWGG 7 cut(s) 503, 810, 1208, 1295, 2083, 2487, 2577
BciVI GTATCC 2 cut(s) 225, 1030
BclI TGATCA 1 cut(s) 880
BcnI CCSGG 1 cut(s) 203
BcoDI GTCTC 3 cut(s) 633, 887, 1177
BcuI ACTAGT 2 cut(s) 1442, 2323
BfmI CTRYAG 3 cut(s) 1109, 1629, 1734
BfuAI ACCTGC 1 cut(s) 1241
BfuI GTATCC 2 cut(s) 225, 1030
BglII AGATCT 3 cut(s) 643, 752, 1570
BisI GCNGC 5 cut(s) 437, 1109, 1351, 1737, 2582
BlsI GCNGC 5 cut(s) 438, 1110, 1352, 1738, 2583
BmcAI AGTACT 2 cut(s) 1497, 2116
Bme1390I CCNGG 8 cut(s) 203, 503, 810, 1208, 1295, 2083, 2487, 2577
Bme18I GGWCC 6 cut(s) 299, 845, 902, 1376, 1592, 1676
BmeRI GACNNNNNGTC 1 cut(s) 900
BmgBI CACGTC 2 cut(s) 858, 1187
BmgT120I GGNCC 7 cut(s) 299, 845, 902, 1291, 1376, 1592, 1676
BmiI GGNNCC 2 cut(s) 1850, 2574
BmrFI CCNGG 8 cut(s) 203, 503, 810, 1208, 1295, 2083, 2487, 2577
BmrI ACTGGG 1 cut(s) 2512
BmsI GCATC 2 cut(s) 1748, 2593
BmuI ACTGGG 1 cut(s) 2512
BpiI GAAGAC 3 cut(s) 702, 852, 2189
BpmI CTGGAG 1 cut(s) 165
Bpu10I CCTNAGC 1 cut(s) 1050
Bpu14I TTCGAA 1 cut(s) 1223
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 887
BsaJI CCNNGG 6 cut(s) 270, 808, 898, 1207, 1293, 2576
BsaWI WCCGGW 4 cut(s) 80, 1059, 2149, 2247
Bsc4I CCNNNNNNNGG 4 cut(s) 201, 1364, 2254, 2382
Bse1I ACTGG 5 cut(s) 372, 928, 1557, 2025, 2507
Bse21I CCTNAGG 1 cut(s) 1359
Bse3DI GCAATG 2 cut(s) 805, 955
BseAI TCCGGA 1 cut(s) 1059
BseBI CCWGG 7 cut(s) 503, 810, 1208, 1295, 2083, 2487, 2577
BseDI CCNNGG 6 cut(s) 270, 808, 898, 1207, 1293, 2576
BseGI GGATG 7 cut(s) 817, 982, 1435, 1739, 1931, 2052, 2608
BseLI CCNNNNNNNGG 4 cut(s) 201, 1364, 2254, 2382
BseMI GCAATG 2 cut(s) 805, 955
BseMII CTCAG 6 cut(s) 335, 863, 993, 1041, 1350, 1824
BseNI ACTGG 5 cut(s) 372, 928, 1557, 2025, 2507
BseRI GAGGAG 1 cut(s) 353
BseSI GKGCMC 1 cut(s) 2396
BseXI GCAGC 5 cut(s) 423, 1095, 1337, 1748, 2593
BseYI CCCAGC 1 cut(s) 1743
BsgI GTGCAG 1 cut(s) 1813
Bsh1236I CGCG 3 cut(s) 2166, 2313, 2431
BshFI GGCC 5 cut(s) 12, 1292, 2024, 2081, 2236
BsiHKAI GWGCWC 1 cut(s) 2396
BsiSI CCGG 5 cut(s) 81, 202, 1060, 2150, 2248
BslFI GGGAC 2 cut(s) 79, 1357
BslI CCNNNNNNNGG 4 cut(s) 201, 1364, 2254, 2382
BsmAI GTCTC 3 cut(s) 633, 887, 1177
BsmBI CGTCTC 2 cut(s) 633, 1177
BsmFI GGGAC 2 cut(s) 79, 1357
BsmI GAATGC 1 cut(s) 2448
BsnI GGCC 5 cut(s) 12, 1292, 2024, 2081, 2236
Bso31I GGTCTC 1 cut(s) 887
Bsp119I TTCGAA 1 cut(s) 1223
Bsp1286I GDGCHC 1 cut(s) 2396
Bsp13I TCCGGA 1 cut(s) 1059
Bsp1407I TGTACA 1 cut(s) 2005
Bsp143I GATC 9 cut(s) 643, 752, 880, 1045, 1071, 1570, 1885, 2527, 2572
BspACI CCGC 4 cut(s) 153, 539, 2126, 2166
BspANI GGCC 5 cut(s) 12, 1292, 2024, 2081, 2236
BspCNI CTCAG 6 cut(s) 336, 862, 994, 1042, 1351, 1825
BspEI TCCGGA 1 cut(s) 1059
BspFNI CGCG 3 cut(s) 2166, 2313, 2431
BspLI GGNNCC 2 cut(s) 1850, 2574
BspMAI CTGCAG 2 cut(s) 1113, 1738
BspMI ACCTGC 1 cut(s) 1241
BspPI GGATC 5 cut(s) 1040, 1079, 2522, 2567, 2580
BspT104I TTCGAA 1 cut(s) 1223
BspTNI GGTCTC 1 cut(s) 887
BsrDI GCAATG 2 cut(s) 805, 955
BsrGI TGTACA 1 cut(s) 2005
BsrI ACTGG 5 cut(s) 372, 928, 1557, 2025, 2507
BssECI CCNNGG 6 cut(s) 270, 808, 898, 1207, 1293, 2576
BssMI GATC 9 cut(s) 643, 752, 880, 1045, 1071, 1570, 1885, 2527, 2572
BssNAI GTATAC 1 cut(s) 1008
BssT1I CCWWGG 2 cut(s) 270, 898
Bst1107I GTATAC 1 cut(s) 1008
Bst2UI CCWGG 7 cut(s) 503, 810, 1208, 1295, 2083, 2487, 2577
Bst4CI ACNGT 4 cut(s) 318, 1012, 1129, 2473
Bst6I CTCTTC 3 cut(s) 388, 1702, 2352
BstAPI GCANNNNNTGC 2 cut(s) 1898, 2603
BstAUI TGTACA 1 cut(s) 2005
BstBI TTCGAA 1 cut(s) 1223
BstC8I GCNNGC 1 cut(s) 14
BstDEI CTNAG 7 cut(s) 344, 849, 1002, 1050, 1088, 1359, 1833
BstEII GGTNACC 1 cut(s) 602
BstF5I GGATG 7 cut(s) 817, 982, 1435, 1739, 1931, 2052, 2608
BstFNI CGCG 3 cut(s) 2166, 2313, 2431
BstHHI GCGC 1 cut(s) 2166
BstKTI GATC 9 cut(s) 646, 755, 883, 1048, 1074, 1573, 1888, 2530, 2575
BstMAI GTCTC 3 cut(s) 633, 887, 1177
BstMBI GATC 9 cut(s) 643, 752, 880, 1045, 1071, 1570, 1885, 2527, 2572
BstMWI GCNNNNNNNGC 5 cut(s) 1397, 1775, 1898, 2259, 2603
BstNI CCWGG 7 cut(s) 503, 810, 1208, 1295, 2083, 2487, 2577
BstPI GGTNACC 1 cut(s) 602
BstSCI CCNGG 8 cut(s) 201, 501, 808, 1206, 1293, 2081, 2485, 2575
BstSFI CTRYAG 3 cut(s) 1109, 1629, 1734
BstSLI GKGCMC 1 cut(s) 2396
BstUI CGCG 3 cut(s) 2166, 2313, 2431
BstV1I GCAGC 5 cut(s) 423, 1095, 1337, 1748, 2593
BstV2I GAAGAC 3 cut(s) 702, 852, 2189
BstX2I RGATCY 6 cut(s) 643, 752, 1045, 1071, 1570, 2572
BstYI RGATCY 6 cut(s) 643, 752, 1045, 1071, 1570, 2572
BstZ17I GTATAC 1 cut(s) 1008
Bsu36I CCTNAGG 1 cut(s) 1359
BsuI GTATCC 2 cut(s) 225, 1030
BsuRI GGCC 5 cut(s) 12, 1292, 2024, 2081, 2236
BtrI CACGTC 2 cut(s) 858, 1187
BtsCI GGATG 7 cut(s) 817, 982, 1435, 1739, 1931, 2052, 2608
BtsIMutI CAGTG 5 cut(s) 314, 379, 1402, 1830, 2233
BveI ACCTGC 1 cut(s) 1241
Cac8I GCNNGC 1 cut(s) 14
CaiI CAGNNNCTG 1 cut(s) 1049
CfoI GCGC 1 cut(s) 2166
Cfr13I GGNCC 7 cut(s) 299, 845, 902, 1291, 1376, 1592, 1676
Csp6I GTAC 7 cut(s) 1355, 1368, 1496, 1692, 2006, 2115, 2556
CspCI CAANNNNNGTGG 2 cut(s) 580, 615
CviAII CATG 4 cut(s) 95, 1982, 2161, 2300
CviQI GTAC 7 cut(s) 1355, 1368, 1496, 1692, 2006, 2115, 2556
DdeI CTNAG 7 cut(s) 344, 849, 1002, 1050, 1088, 1359, 1833
DpnI GATC 9 cut(s) 645, 754, 882, 1047, 1073, 1572, 1887, 2529, 2574
DpnII GATC 9 cut(s) 643, 752, 880, 1045, 1071, 1570, 1885, 2527, 2572
DriI GACNNNNNGTC 1 cut(s) 900
EaeI YGGCCR 1 cut(s) 2079
Eam1104I CTCTTC 3 cut(s) 388, 1702, 2352
Eam1105I GACNNNNNGTC 1 cut(s) 900
EarI CTCTTC 3 cut(s) 388, 1702, 2352
Eco130I CCWWGG 2 cut(s) 270, 898
Eco31I GGTCTC 1 cut(s) 887
Eco32I GATATC 1 cut(s) 419
Eco47I GGWCC 6 cut(s) 299, 845, 902, 1376, 1592, 1676
Eco57I CTGAAG 4 cut(s) 712, 747, 1026, 1121
Eco81I CCTNAGG 1 cut(s) 1359
Eco91I GGTNACC 1 cut(s) 602
EcoO109I RGGNCCY 1 cut(s) 299
EcoO65I GGTNACC 1 cut(s) 602
EcoRI GAATTC 2 cut(s) 1212, 2345
EcoRII CCWGG 7 cut(s) 501, 808, 1206, 1293, 2081, 2485, 2575
EcoRV GATATC 1 cut(s) 419
EcoT14I CCWWGG 2 cut(s) 270, 898
ErhI CCWWGG 2 cut(s) 270, 898
Esp3I CGTCTC 2 cut(s) 633, 1177
FaeI CATG 4 cut(s) 98, 1985, 2164, 2303
FalI AAGNNNNNCTT 4 cut(s) 664, 696, 1680, 1712
FaqI GGGAC 2 cut(s) 79, 1357
FatI CATG 4 cut(s) 94, 1981, 2160, 2299
FauI CCCGC 1 cut(s) 2119
FbaI TGATCA 1 cut(s) 880
FblI GTMKAC 3 cut(s) 88, 1007, 1122
Fnu4HI GCNGC 5 cut(s) 437, 1109, 1351, 1737, 2582
FokI GGATG 7 cut(s) 804, 989, 1422, 1726, 1918, 2039, 2615
Fsp4HI GCNGC 5 cut(s) 437, 1109, 1351, 1737, 2582
GlaI GCGC 1 cut(s) 2165
GluI GCNGC 5 cut(s) 437, 1109, 1351, 1737, 2582
GsaI CCCAGC 1 cut(s) 1747
GsuI CTGGAG 1 cut(s) 165
HaeIII GGCC 5 cut(s) 12, 1292, 2024, 2081, 2236
HapII CCGG 5 cut(s) 81, 202, 1060, 2150, 2248
HhaI GCGC 1 cut(s) 2166
Hin1II CATG 4 cut(s) 98, 1985, 2164, 2303
Hin6I GCGC 1 cut(s) 2164
HinP1I GCGC 1 cut(s) 2164
HincII GTYRAC 1 cut(s) 805
HindII GTYRAC 1 cut(s) 805
HindIII AAGCTT 3 cut(s) 260, 511, 673
HpaII CCGG 5 cut(s) 81, 202, 1060, 2150, 2248
HphI GGTGA 4 cut(s) 167, 491, 700, 2397
Hpy166II GTNNAC 9 cut(s) 89, 691, 805, 855, 1008, 1123, 2008, 2329, 2394
Hpy8I GTNNAC 9 cut(s) 89, 691, 805, 855, 1008, 1123, 2008, 2329, 2394
Hpy99I CGWCG 1 cut(s) 172
HpyCH4III ACNGT 4 cut(s) 318, 1012, 1129, 2473
HpyCH4IV ACGT 3 cut(s) 170, 857, 1186
HpyF10VI GCNNNNNNNGC 5 cut(s) 1397, 1775, 1898, 2259, 2603
HpyF3I CTNAG 7 cut(s) 344, 849, 1002, 1050, 1088, 1359, 1833
HpySE526I ACGT 3 cut(s) 170, 857, 1186
Hsp92II CATG 4 cut(s) 98, 1985, 2164, 2303
HspAI GCGC 1 cut(s) 2164
Kpn2I TCCGGA 1 cut(s) 1059
Ksp22I TGATCA 1 cut(s) 880
Kzo9I GATC 9 cut(s) 643, 752, 880, 1045, 1071, 1570, 1885, 2527, 2572
LmnI GCTCC 3 cut(s) 340, 1854, 2250
Lsp1109I GCAGC 5 cut(s) 423, 1095, 1337, 1748, 2593
LweI GCATC 2 cut(s) 1748, 2593
MaeII ACGT 3 cut(s) 170, 857, 1186
MaeIII GTNAC 4 cut(s) 602, 919, 2473, 2489
MalI GATC 9 cut(s) 645, 754, 882, 1047, 1073, 1572, 1887, 2529, 2574
MboI GATC 9 cut(s) 643, 752, 880, 1045, 1071, 1570, 1885, 2527, 2572
MboII GAAGA 9 cut(s) 375, 707, 852, 1462, 1580, 1719, 2194, 2267, 2369
MfeI CAATTG 2 cut(s) 1727, 2133
MflI RGATCY 6 cut(s) 643, 752, 1045, 1071, 1570, 2572
MhlI GDGCHC 1 cut(s) 2396
MlsI TGGCCA 1 cut(s) 2081
MluNI TGGCCA 1 cut(s) 2081
MlyI GAGTC 3 cut(s) 1888, 2036, 2324
MmeI TCCRAC 1 cut(s) 1463
Mox20I TGGCCA 1 cut(s) 2081
MroI TCCGGA 1 cut(s) 1059
MroXI GAANNNNTTC 1 cut(s) 2446
MscI TGGCCA 1 cut(s) 2081
MslI CAYNNNNRTG 2 cut(s) 2013, 2298
Msp20I TGGCCA 1 cut(s) 2081
MspI CCGG 5 cut(s) 81, 202, 1060, 2150, 2248
MspR9I CCNGG 8 cut(s) 203, 503, 810, 1208, 1295, 2083, 2487, 2577
MunI CAATTG 2 cut(s) 1727, 2133
Mva1269I GAATGC 1 cut(s) 2448
MvaI CCWGG 7 cut(s) 503, 810, 1208, 1295, 2083, 2487, 2577
MvnI CGCG 3 cut(s) 2166, 2313, 2431
MwoI GCNNNNNNNGC 5 cut(s) 1397, 1775, 1898, 2259, 2603
NciI CCSGG 1 cut(s) 203
NdeII GATC 9 cut(s) 643, 752, 880, 1045, 1071, 1570, 1885, 2527, 2572
NlaIII CATG 4 cut(s) 98, 1985, 2164, 2303
NlaIV GGNNCC 2 cut(s) 1850, 2574
NmuCI GTSAC 1 cut(s) 919
NspV TTCGAA 1 cut(s) 1223
PaqCI CACCTGC 1 cut(s) 1241
PctI GAATGC 1 cut(s) 2448
PdmI GAANNNNTTC 1 cut(s) 2446
PfeI GAWTC 7 cut(s) 160, 458, 813, 1504, 1824, 1918, 1988
PkrI GCNGC 5 cut(s) 438, 1110, 1352, 1738, 2583
PleI GAGTC 3 cut(s) 1887, 2036, 2324
PpsI GAGTC 3 cut(s) 1887, 2036, 2324
PpuMI RGGWCCY 1 cut(s) 299
Psp5II RGGWCCY 1 cut(s) 299
Psp6I CCWGG 7 cut(s) 501, 808, 1206, 1293, 2081, 2485, 2575
PspEI GGTNACC 1 cut(s) 602
PspFI CCCAGC 1 cut(s) 1743
PspGI CCWGG 7 cut(s) 501, 808, 1206, 1293, 2081, 2485, 2575
PspN4I GGNNCC 2 cut(s) 1850, 2574
PspPI GGNCC 7 cut(s) 299, 845, 902, 1291, 1376, 1592, 1676
PspPPI RGGWCCY 1 cut(s) 299
PstI CTGCAG 2 cut(s) 1113, 1738
PstNI CAGNNNCTG 1 cut(s) 1049
PsuI RGATCY 6 cut(s) 643, 752, 1045, 1071, 1570, 2572
RsaI GTAC 7 cut(s) 1356, 1369, 1497, 1693, 2007, 2116, 2557
RsaNI GTAC 7 cut(s) 1355, 1368, 1496, 1692, 2006, 2115, 2556
RseI CAYNNNNRTG 2 cut(s) 2013, 2298
SatI GCNGC 5 cut(s) 437, 1109, 1351, 1737, 2582
Sau3AI GATC 9 cut(s) 643, 752, 880, 1045, 1071, 1570, 1885, 2527, 2572
Sau96I GGNCC 7 cut(s) 299, 845, 902, 1291, 1376, 1592, 1676
ScaI AGTACT 2 cut(s) 1497, 2116
SchI GAGTC 3 cut(s) 1888, 2036, 2324
ScrFI CCNGG 8 cut(s) 203, 503, 810, 1208, 1295, 2083, 2487, 2577
SduI GDGCHC 1 cut(s) 2396
SfaNI GCATC 2 cut(s) 1748, 2593
SfcI CTRYAG 3 cut(s) 1109, 1629, 1734
SfuI TTCGAA 1 cut(s) 1223
SinI GGWCC 6 cut(s) 299, 845, 902, 1376, 1592, 1676
SmiMI CAYNNNNRTG 2 cut(s) 2013, 2298
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
SpeI ACTAGT 2 cut(s) 1442, 2323
SsiI CCGC 4 cut(s) 153, 539, 2126, 2166
SspI AATATT 4 cut(s) 451, 475, 2032, 2620
StyD4I CCNGG 8 cut(s) 201, 501, 808, 1206, 1293, 2081, 2485, 2575
StyI CCWWGG 2 cut(s) 270, 898
TaaI ACNGT 4 cut(s) 318, 1012, 1129, 2473
TaiI ACGT 3 cut(s) 173, 860, 1189
TaqI TCGA 3 cut(s) 1223, 2040, 2280
TatI WGTACW 4 cut(s) 1495, 2005, 2114, 2555
TfiI GAWTC 7 cut(s) 160, 458, 813, 1504, 1824, 1918, 1988
TscAI CASTG 5 cut(s) 321, 379, 1402, 1837, 2233
TseFI GTSAC 1 cut(s) 919
TseI GCWGC 5 cut(s) 436, 1108, 1350, 1736, 2581
Tsp45I GTSAC 1 cut(s) 919
TspDTI ATGAA 7 cut(s) 86, 422, 1071, 1310, 1581, 2316, 2603
TspGWI ACGGA 2 cut(s) 1049, 2615
TspRI CASTG 5 cut(s) 321, 379, 1402, 1837, 2233
VneI GTGCAC 1 cut(s) 2392
VpaK11BI GGWCC 6 cut(s) 299, 845, 902, 1376, 1592, 1676
XapI RAATTY 9 cut(s) 106, 115, 739, 996, 1212, 1317, 1813, 2239, 2345
XbaI TCTAGA 1 cut(s) 2215
XmiI GTMKAC 3 cut(s) 88, 1007, 1122
XmnI GAANNNNTTC 1 cut(s) 2446
ZrmI AGTACT 2 cut(s) 1497, 2116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.