RchiOBHm_Chr5g0025931
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000088
Physical Location & Seq
Forward (+)
19903762 .. 19915073
11312 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30553

Sequence Viewer

Length: 3192 bp
ATGGTTGCATTATCTTCTGGACATCTAGTCATTAAAATTCTCAGTGTCCATATTCAGCTAGCAAATCTACAGAGCAAGATCAGATATACTATATTCATGTCAGGAAGCTCCGTCGTCTCATCATCAGTATGGGGGTTATTCTTGTTCATCTTTGGGCTGTGTGTTCTGGTAGAAGCTCAAGCTCCTTCTTCTCAACCTTCTCAAGCTCCTAGTTCTCAACCTCAAGCTACCACTGATCCATTTGAAGCGAGAGCTCTGAATTCAGTCTTTCAAAAATGGAAAATTGCACCCAAACAGAGGCAATGGAACATAAGCGGAGAACCATGCAGTGGAGCAGCCATAGACCCCTTCATCTTTGCCAGTAATGACTCCTACAACCCCTTAATCAAATGTGTCTGTTCCAACTTCACTTGCCACATTACTGAACTGAAAGTTTATGGCTTGGACGTTATTGGTGAACTCCCAGAAGAGCTTTGGAGTTTGACTTCCCTCACCAGCCTGAATTTGGCCCAGAATTACTTGACAGGACCTCTCTCTCCATCAATTGGAAATCTTACCGACATGCAATACTTGAGCCTTGGCATTAATTCATTATCAGGCGAGATACCGAAGGAACTCGGGAAGCTCACTGATTTAATATCATTGGCTTTTGGGACAAACAACTTTTCTGGTCCCCTGCCATCTGAGCTAGGGAATTTGACAAAATTAGAACAGATTTACATTGATAGTTCTGGAGCTGGTGGCGCAATTCCATCAACATTTGCTAATCTACAAAGCTTGAAGACAGTTTGGGCATCAGACAATGAATTCACTGGCAGGATACCAGACTTCATAGGAAATTGGTCAAGTCTTACAACCTTGTTGTTTCATGGTAATTCTTTTGAAGGTCCTATTCCATTGACACTTTCCAATCTGTCATCTCTAACAGAGCTGCGTATCAGTGATGTATCTAATGGTAATAGGAGCTCTTCTCTTGGATTTATAAAGAATATGAAGTCTCTGAATATCTTAGTGCTGAGGAATAACCATATTTCTGACGTAATTCCTTCCTACATTGGAGATTACCAACGTTTGTCTCAGCTGGATTTGAGCTTCAACGATATAACTGGACGAATACCAGACTCGCTGTTTAATTTGAGTTTGCTCAGTATCCTGTTTCTCGGAAACAACAAGTTGAATGGTACCCTTCCTGAATCTAAAAAATCCTCTCTTCACACTGTAGACCTATCTTACAATAATCTAGTCGGGAGATTTCCTTCTTGGGTGAATGAAGAGTATTTACAGCTTAACTTAGTTGCCAACAACTTTAGCATCGAAACTTCAAACAGCAGTGTTCTGCCTTCAGGTTTGATTTGTCTTCAAAAAAACTTTCCCTGCATTCGCGGCCCTGGAATCAATTATAATTTTGGGATCAAATGTGGTGGTCCTCAGGTTAGATCTTCCAATGGAATTGTGTATGACAAGGAAAATGAGACCCTTGGTCCAGCTACATATATGGTCAATGACGAGAACAGATGGGGAGTTAGCAACACTGGAATTTTCACTCGGAGCAATAACAATCAGTATACAATTAACTCCTCCCTTCAATTCACAAGTACTTTAGATTCTGTGCTATTCCAAACTGCAAGACTCTCTGCTTCGTCACTAAGATATTATGGCTTGGGGCTTCAGAATGGTAATTACACCGTGAACCTCCAATTTGCAGAACAAGTTATCCTAGATTCTACTTCTTGGAGAAGTCTTGGGAGACGTGTTTTTGATATTTATATTCAGGGTATCCTGGTTTTGAAGGATTTTGATATACGAAAGGAGGCAGGGGCATCTCTCCTACCTCTTCGGAAGGAATACAAGGCTCATGTTTCAGAGAACTACCTTGAAATCCATCTCTTTTGGGCTGGAAAGGGGACTTGTTGTGTACCTGTTCCTGGTACTTATGGACCTGCTATTTCAGCCATCAGGGCCACACCAGATTTCATTCCTGATGACAATAACAATAATACAAATGAAAAGAAGATTAGGACATGGTTGATTGTGGGGTTTGCTATTGGTGGTGGAGTTTTACTTCTGGTGATGTCAATTTTCCTTATTGTTCGAAGAAGAAAAAGCCCCAAATCCTGTGATGATGAAGAGCTGCTAGGAATAGATGTTGGACGACTCGCTATCAGTTATTCTGAACTAAAGACAGCTACAAATGACTTCGATCCATCGAATAAACTTGGAGAGGGAGGATTTGGTCCTGTGTACAAAGGATTACTTAATGATGGAAGAGTTATTGCCGTGAAGCAATTGTCTGCAGCATCCCATCAAGGAAAGAACCAGTTTATAACTGAGATTGCTACTATATCAGCAGTGCAACACAATAACCTAGTGACTTTGTATGGATTCTGCACTGAGGGAGATAAACGACTCCTTGTGTATGAGTATCTAGTGAACAAGAGTCTTGATCAAGCACTATTTGGAGAAAGAGGTTTGAATCTAGATTGGTCCACACGGTTTGGTATATGCATGGGAGTAGCTAGAGGTTTGAACTATCTTCATGAGGAATCAAGGCTTCGAATTGTACACAGAGATGTGAAGGCCAGTAATATTCTGCTCGATTCTGAACTCATCCCCAAAATATCAGATTTTGGTTTAGCCAAGCTTTATGATAGTAAAAAGACCCACATGAGTACCCAGGTTGCTGGGACACTTGGGTATCTTGCACCGGAATATGCCATGCGTGGCCATCTTACAGAGAAGACTGATGTCTTTGCCTTCGGTGTTGTGGCTCTAGAAATTGTCAGCGGTAGACCCAATTGTGACACGAGCTTAGACGAAGACAAGATTTATCTTCTTGAATCGGCTTGGGTCTTGCATGAAAGCAAACGTGAAGTTGAACTAGTGGACTCTGGATTATCTGAATTTGACGAGGAAGAAGTGAGACGAGTAATCAACATAGGACTTTTGTGCACTCAAACATCACCAACACTACGACCATCAATGTCTCGTGTGGTGGGAATGCTTTCAGGAGACATTGAAGTGACGATATTAACTTCAAAGCCCGGTTATTTGACAGACTGGAAGTTTGATGATGTAAGTAGTGTAGCCAGCACCATGATGGATATGCCAAGTAACGGAATAAATACTAGCCTTTTCAACTCAACAGCATGTACAAGCTTGATGGGGGATGCACAGCAATCACCTGCAAATTAA

Protein Analysis

1063

Amino Acids

116.66

Weight (kDa)

5.62

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 143 - 287 9.8e-11 Leucine-rich repeat region
LRR_14 PF23598 302 - 391 7.5e-06 Leucine-rich repeat region
LRR_8 PF13855 333 - 392 1.4e-06 Leucine rich repeat
Malectin PF11721 469 - 652 8.6e-40 Malectin domain
Pkinase PF00069 736 - 995 4.9e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 737 - 998 5.9e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 983, 1401, 2324
Acc36I ACCTGC 1 cut(s) 1948
Acc65I GGTACC 1 cut(s) 1181
AccB1I GGYRCC 1 cut(s) 1181
AccB7I CCANNNNNTGG 2 cut(s) 329, 545
AccI GTMKAC 3 cut(s) 1221, 1565, 2788
AccII CGCG 1 cut(s) 1383
AciI CCGC 3 cut(s) 315, 1383, 2784
AclI AACGTT 1 cut(s) 1069
AclWI GGATC 3 cut(s) 230, 1418, 2195
AcoI YGGCCR 1 cut(s) 2722
AcsI RAATTY 7 cut(s) 36, 259, 502, 694, 806, 1536, 2900
AcuI CTGAAG 2 cut(s) 1326, 1652
AfaI GTAC 8 cut(s) 1183, 1597, 1917, 1930, 2243, 2562, 2671, 3151
AfiI CCNNNNNNNGG 6 cut(s) 297, 329, 505, 545, 1925, 3113
AflIII ACRYGT 1 cut(s) 1750
AhdI GACNNNNNGTC 2 cut(s) 26, 1479
AhlI ACTAGT 1 cut(s) 2878
AjiI CACGTC 1 cut(s) 1751
AjnI CCWGG 4 cut(s) 1387, 1779, 1924, 2673
AloI GAACNNNNNNTCC 2 cut(s) 1698, 1730
Alw21I GWGCWC 3 cut(s) 256, 968, 2951
Alw26I GTCTC 8 cut(s) 121, 1002, 1080, 1466, 1741, 2914, 2988, 3003
Alw44I GTGCAC 1 cut(s) 2947
AlwI GGATC 3 cut(s) 230, 1418, 2195
Ama87I CYCGRG 1 cut(s) 617
AoxI GGCC 5 cut(s) 507, 1384, 1959, 2577, 2722
ApaLI GTGCAC 1 cut(s) 2947
ApeKI GCWGC 4 cut(s) 335, 931, 2131, 2294
ApoI RAATTY 7 cut(s) 36, 259, 502, 694, 806, 1536, 2900
ArsI GACNNNNNNTTYG 2 cut(s) 3047, 3079
AseI ATTAAT 1 cut(s) 585
Asp700I GAANNNNTTC 1 cut(s) 3001
Asp718I GGTACC 1 cut(s) 1181
AspLEI GCGC 1 cut(s) 746
AsuC2I CCSGG 1 cut(s) 3042
AsuHPI GGTGA 6 cut(s) 467, 484, 1276, 2080, 2952, 3171
AsuII TTCGAA 2 cut(s) 2092, 2554
AsuNHI GCTAGC 1 cut(s) 58
AvaI CYCGRG 1 cut(s) 617
AvaII GGWCC 8 cut(s) 527, 671, 887, 1424, 1481, 1937, 2234, 2484
AxyI CCTNAGG 1 cut(s) 1428
BaeGI GKGCMC 1 cut(s) 2951
BaeI ACNNNNGTAYC 4 cut(s) 2653, 2678, 2686, 2711
BalI TGGCCA 1 cut(s) 2724
BanI GGYRCC 1 cut(s) 1181
BanII GRGCYC 2 cut(s) 256, 968
BarI GAAGNNNNNNTAC 2 cut(s) 1263, 1295
BauI CACGAG 2 cut(s) 2803, 2985
BbsI GAAGAC 4 cut(s) 788, 1349, 2744, 2823
Bbv12I GWGCWC 3 cut(s) 256, 968, 2951
BbvCI CCTCAGC 1 cut(s) 1016
BbvI GCAGC 4 cut(s) 347, 918, 2118, 2306
BceAI ACGGC 1 cut(s) 2261
BciT130I CCWGG 4 cut(s) 1389, 1781, 1926, 2675
BciVI GTATCC 3 cut(s) 813, 1160, 1787
BclI TGATCA 1 cut(s) 2443
BcnI CCSGG 1 cut(s) 3042
BcoDI GTCTC 8 cut(s) 121, 1002, 1080, 1466, 1741, 2914, 2988, 3003
BcuI ACTAGT 1 cut(s) 2878
BfmI CTRYAG 3 cut(s) 68, 1218, 2292
BfuAI ACCTGC 1 cut(s) 1948
BfuI GTATCC 3 cut(s) 813, 1160, 1787
BglI GCCNNNNNGGC 1 cut(s) 1958
BglII AGATCT 1 cut(s) 1436
BisI GCNGC 5 cut(s) 336, 932, 1384, 2132, 2295
BlsI GCNGC 5 cut(s) 337, 933, 1385, 2133, 2296
BmcAI AGTACT 1 cut(s) 1597
Bme1390I CCNGG 5 cut(s) 1389, 1781, 1926, 2675, 3042
Bme18I GGWCC 8 cut(s) 527, 671, 887, 1424, 1481, 1937, 2234, 2484
BmeRI GACNNNNNGTC 2 cut(s) 26, 1479
BmeT110I CYCGRG 1 cut(s) 617
BmgBI CACGTC 1 cut(s) 1751
BmiI GGNNCC 2 cut(s) 673, 1183
BmrFI CCNGG 5 cut(s) 1389, 1781, 1926, 2675, 3042
BmsI GCATC 5 cut(s) 803, 1320, 1829, 2306, 3157
BmtI GCTAGC 1 cut(s) 62
BoxI GACNNNNGTC 1 cut(s) 2744
BpiI GAAGAC 4 cut(s) 788, 1349, 2744, 2823
BplI GAGNNNNNCTC 2 cut(s) 955, 987
BpmI CTGGAG 1 cut(s) 753
Bpu10I CCTNAGC 1 cut(s) 1016
Bpu14I TTCGAA 2 cut(s) 2092, 2554
BpuEI CTTGAG 4 cut(s) 162, 186, 207, 592
BpuMI CCSGG 1 cut(s) 3042
BsaI GGTCTC 1 cut(s) 1466
BsaJI CCNNGG 4 cut(s) 577, 1387, 1477, 2673
BsaWI WCCGGW 1 cut(s) 2704
Bsc4I CCNNNNNNNGG 6 cut(s) 297, 329, 505, 545, 1925, 3113
Bse1I ACTGG 7 cut(s) 360, 817, 1111, 1537, 2317, 2580, 3062
Bse21I CCTNAGG 1 cut(s) 1428
Bse3DI GCAATG 1 cut(s) 308
BseBI CCWGG 4 cut(s) 1389, 1781, 1926, 2675
BseDI CCNNGG 4 cut(s) 577, 1387, 1477, 2673
BseGI GGATG 3 cut(s) 2297, 2607, 3172
BseLI CCNNNNNNNGG 6 cut(s) 297, 329, 505, 545, 1925, 3113
BseMI GCAATG 1 cut(s) 308
BseMII CTCAG 8 cut(s) 55, 675, 1007, 1091, 1159, 1442, 2319, 2382
BseNI ACTGG 7 cut(s) 360, 817, 1111, 1537, 2317, 2580, 3062
BseRI GAGGAG 1 cut(s) 1567
BseSI GKGCMC 1 cut(s) 2951
BseXI GCAGC 4 cut(s) 347, 918, 2118, 2306
BseYI CCCAGC 1 cut(s) 2681
BsgI GTGCAG 1 cut(s) 2371
Bsh1236I CGCG 1 cut(s) 1383
BshFI GGCC 5 cut(s) 509, 1386, 1961, 2579, 2724
BshNI GGYRCC 1 cut(s) 1181
BsiHKAI GWGCWC 3 cut(s) 256, 968, 2951
BsiHKCI CYCGRG 1 cut(s) 617
BsiSI CCGG 2 cut(s) 2705, 3042
BslFI GGGAC 4 cut(s) 657, 667, 1918, 2698
BslI CCNNNNNNNGG 6 cut(s) 297, 329, 505, 545, 1925, 3113
BsmAI GTCTC 8 cut(s) 121, 1002, 1080, 1466, 1741, 2914, 2988, 3003
BsmBI CGTCTC 3 cut(s) 121, 1741, 2914
BsmFI GGGAC 4 cut(s) 657, 667, 1918, 2698
BsmI GAATGC 2 cut(s) 1377, 3003
BsnI GGCC 5 cut(s) 509, 1386, 1961, 2579, 2724
Bso31I GGTCTC 1 cut(s) 1466
BsoBI CYCGRG 1 cut(s) 617
Bsp119I TTCGAA 2 cut(s) 2092, 2554
Bsp1286I GDGCHC 3 cut(s) 256, 968, 2951
Bsp1407I TGTACA 3 cut(s) 2241, 2560, 3149
Bsp143I GATC 6 cut(s) 78, 235, 1410, 1436, 2200, 2443
BspACI CCGC 3 cut(s) 315, 1383, 2784
BspANI GGCC 5 cut(s) 509, 1386, 1961, 2579, 2724
BspCNI CTCAG 8 cut(s) 54, 676, 1008, 1090, 1158, 1441, 2320, 2383
BspFNI CGCG 1 cut(s) 1383
BspHI TCATGA 1 cut(s) 2536
BspLI GGNNCC 2 cut(s) 673, 1183
BspMAI CTGCAG 1 cut(s) 2296
BspMI ACCTGC 1 cut(s) 1948
BspOI GCTAGC 1 cut(s) 62
BspPI GGATC 3 cut(s) 230, 1418, 2195
BspQI GCTCTTC 3 cut(s) 462, 973, 2121
BspT104I TTCGAA 2 cut(s) 2092, 2554
BspT107I GGYRCC 1 cut(s) 1181
BspTNI GGTCTC 1 cut(s) 1466
BsrDI GCAATG 1 cut(s) 308
BsrGI TGTACA 3 cut(s) 2241, 2560, 3149
BsrI ACTGG 7 cut(s) 360, 817, 1111, 1537, 2317, 2580, 3062
BssECI CCNNGG 4 cut(s) 577, 1387, 1477, 2673
BssMI GATC 6 cut(s) 78, 235, 1410, 1436, 2200, 2443
BssNAI GTATAC 1 cut(s) 1566
BssSI CACGAG 2 cut(s) 2803, 2985
BssT1I CCWWGG 2 cut(s) 577, 1477
Bst1107I GTATAC 1 cut(s) 1566
Bst2BI CACGAG 2 cut(s) 2803, 2985
Bst2UI CCWGG 4 cut(s) 1389, 1781, 1926, 2675
Bst4CI ACNGT 4 cut(s) 787, 1219, 1687, 2493
Bst6I CTCTTC 7 cut(s) 462, 973, 1215, 1266, 1839, 2121, 2260
BstAUI TGTACA 3 cut(s) 2241, 2560, 3149
BstBI TTCGAA 2 cut(s) 2092, 2554
BstC8I GCNNGC 2 cut(s) 60, 3088
BstF5I GGATG 3 cut(s) 2297, 2607, 3172
BstFNI CGCG 1 cut(s) 1383
BstHHI GCGC 1 cut(s) 746
BstKTI GATC 6 cut(s) 81, 238, 1413, 1439, 2203, 2446
BstMAI GTCTC 8 cut(s) 121, 1002, 1080, 1466, 1741, 2914, 2988, 3003
BstMBI GATC 6 cut(s) 78, 235, 1410, 1436, 2200, 2443
BstMWI GCNNNNNNNGC 5 cut(s) 685, 743, 1383, 1949, 1958
BstNI CCWGG 4 cut(s) 1389, 1781, 1926, 2675
BstNSI RCATGY 2 cut(s) 565, 3150
BstPAI GACNNNNGTC 1 cut(s) 2744
BstSCI CCNGG 5 cut(s) 1387, 1779, 1924, 2673, 3040
BstSFI CTRYAG 3 cut(s) 68, 1218, 2292
BstSLI GKGCMC 1 cut(s) 2951
BstUI CGCG 1 cut(s) 1383
BstV1I GCAGC 4 cut(s) 347, 918, 2118, 2306
BstV2I GAAGAC 4 cut(s) 788, 1349, 2744, 2823
BstX2I RGATCY 1 cut(s) 1436
BstXI CCANNNNNNTGG 1 cut(s) 2681
BstYI RGATCY 1 cut(s) 1436
BstZ17I GTATAC 1 cut(s) 1566
Bsu36I CCTNAGG 1 cut(s) 1428
BsuI GTATCC 3 cut(s) 813, 1160, 1787
BsuRI GGCC 5 cut(s) 509, 1386, 1961, 2579, 2724
BtrI CACGTC 1 cut(s) 1751
BtsCI GGATG 3 cut(s) 2297, 2607, 3172
BtsI GCAGTG 3 cut(s) 334, 1336, 2355
BveI ACCTGC 1 cut(s) 1948
Cac8I GCNNGC 2 cut(s) 60, 3088
CciI TCATGA 1 cut(s) 2536
CfoI GCGC 1 cut(s) 746
Csp6I GTAC 8 cut(s) 1182, 1596, 1916, 1929, 2242, 2561, 2670, 3150
CspCI CAANNNNNGTGG 4 cut(s) 1402, 1437, 2476, 2511
CviQI GTAC 8 cut(s) 1182, 1596, 1916, 1929, 2242, 2561, 2670, 3150
DpnI GATC 6 cut(s) 80, 237, 1412, 1438, 2202, 2445
DpnII GATC 6 cut(s) 78, 235, 1410, 1436, 2200, 2443
DriI GACNNNNNGTC 2 cut(s) 26, 1479
EaeI YGGCCR 1 cut(s) 2722
Eam1104I CTCTTC 7 cut(s) 462, 973, 1215, 1266, 1839, 2121, 2260
Eam1105I GACNNNNNGTC 2 cut(s) 26, 1479
EarI CTCTTC 7 cut(s) 462, 973, 1215, 1266, 1839, 2121, 2260
Ecl136II GAGCTC 2 cut(s) 254, 966
Eco130I CCWWGG 2 cut(s) 577, 1477
Eco24I GRGCYC 2 cut(s) 256, 968
Eco31I GGTCTC 1 cut(s) 1466
Eco47I GGWCC 8 cut(s) 527, 671, 887, 1424, 1481, 1937, 2234, 2484
Eco53kI GAGCTC 2 cut(s) 254, 966
Eco57I CTGAAG 2 cut(s) 1326, 1652
Eco81I CCTNAGG 1 cut(s) 1428
Eco88I CYCGRG 1 cut(s) 617
EcoICRI GAGCTC 2 cut(s) 254, 966
EcoO109I RGGNCCY 2 cut(s) 527, 887
EcoRI GAATTC 2 cut(s) 259, 806
EcoRII CCWGG 4 cut(s) 1387, 1779, 1924, 2673
EcoT14I CCWWGG 2 cut(s) 577, 1477
EcoT22I ATGCAT 1 cut(s) 2507
EcoT38I GRGCYC 2 cut(s) 256, 968
ErhI CCWWGG 2 cut(s) 577, 1477
Esp3I CGTCTC 3 cut(s) 121, 1741, 2914
FalI AAGNNNNNCTT 2 cut(s) 2238, 2270
FaqI GGGAC 4 cut(s) 657, 667, 1918, 2698
FbaI TGATCA 1 cut(s) 2443
FblI GTMKAC 3 cut(s) 1221, 1565, 2788
Fnu4HI GCNGC 5 cut(s) 336, 932, 1384, 2132, 2295
FokI GGATG 3 cut(s) 2284, 2594, 3179
FriOI GRGCYC 2 cut(s) 256, 968
Fsp4HI GCNGC 5 cut(s) 336, 932, 1384, 2132, 2295
GlaI GCGC 1 cut(s) 745
GluI GCNGC 5 cut(s) 336, 932, 1384, 2132, 2295
GsaI CCCAGC 1 cut(s) 2685
GsuI CTGGAG 1 cut(s) 753
HaeIII GGCC 5 cut(s) 509, 1386, 1961, 2579, 2724
HapII CCGG 2 cut(s) 2705, 3042
HhaI GCGC 1 cut(s) 746
Hin6I GCGC 1 cut(s) 744
HinP1I GCGC 1 cut(s) 744
HindIII AAGCTT 3 cut(s) 775, 2639, 3154
HpaII CCGG 2 cut(s) 2705, 3042
HphI GGTGA 6 cut(s) 467, 484, 1276, 2080, 2952, 3171
Hpy99I CGWCG 1 cut(s) 116
HpyCH4III ACNGT 4 cut(s) 787, 1219, 1687, 2493
HpyCH4IV ACGT 5 cut(s) 447, 1038, 1069, 1750, 2866
HpyF10VI GCNNNNNNNGC 5 cut(s) 685, 743, 1383, 1949, 1958
HpySE526I ACGT 5 cut(s) 447, 1038, 1069, 1750, 2866
HspAI GCGC 1 cut(s) 744
KpnI GGTACC 1 cut(s) 1185
Ksp22I TGATCA 1 cut(s) 2443
Kzo9I GATC 6 cut(s) 78, 235, 1410, 1436, 2200, 2443
LguI GCTCTTC 3 cut(s) 462, 973, 2121
LmnI GCTCC 7 cut(s) 113, 187, 211, 332, 734, 963, 1548
Lsp1109I GCAGC 4 cut(s) 347, 918, 2118, 2306
LweI GCATC 5 cut(s) 803, 1320, 1829, 2306, 3157
MaeII ACGT 5 cut(s) 447, 1038, 1069, 1750, 2866
MaeIII GTNAC 5 cut(s) 1641, 2368, 2798, 3019, 3110
MalI GATC 6 cut(s) 80, 237, 1412, 1438, 2202, 2445
MboI GATC 6 cut(s) 78, 235, 1410, 1436, 2200, 2443
MfeI CAATTG 3 cut(s) 543, 2285, 2794
MflI RGATCY 1 cut(s) 1436
MhlI GDGCHC 3 cut(s) 256, 968, 2951
MlsI TGGCCA 1 cut(s) 2724
MluNI TGGCCA 1 cut(s) 2724
MlyI GAGTC 7 cut(s) 362, 1115, 1623, 2148, 2400, 2446, 2879
MmeI TCCRAC 2 cut(s) 426, 2128
Mox20I TGGCCA 1 cut(s) 2724
Mph1103I ATGCAT 1 cut(s) 2507
MroXI GAANNNNTTC 1 cut(s) 3001
MscI TGGCCA 1 cut(s) 2724
MslI CAYNNNNRTG 4 cut(s) 127, 2568, 3017, 3095
Msp20I TGGCCA 1 cut(s) 2724
MspA1I CMGCKG 2 cut(s) 1081, 2784
MspI CCGG 2 cut(s) 2705, 3042
MspR9I CCNGG 5 cut(s) 1389, 1781, 1926, 2675, 3042
MunI CAATTG 3 cut(s) 543, 2285, 2794
Mva1269I GAATGC 2 cut(s) 1377, 3003
MvaI CCWGG 4 cut(s) 1389, 1781, 1926, 2675
MvnI CGCG 1 cut(s) 1383
MwoI GCNNNNNNNGC 5 cut(s) 685, 743, 1383, 1949, 1958
NciI CCSGG 1 cut(s) 3042
NdeII GATC 6 cut(s) 78, 235, 1410, 1436, 2200, 2443
NheI GCTAGC 1 cut(s) 58
NlaIV GGNNCC 2 cut(s) 673, 1183
NmuCI GTSAC 4 cut(s) 1641, 2368, 2798, 3019
NsiI ATGCAT 1 cut(s) 2507
NspI RCATGY 2 cut(s) 565, 3150
NspV TTCGAA 2 cut(s) 2092, 2554
PagI TCATGA 1 cut(s) 2536
PciSI GCTCTTC 3 cut(s) 462, 973, 2121
PctI GAATGC 2 cut(s) 1377, 3003
PdmI GAANNNNTTC 1 cut(s) 3001
PfeI GAWTC 9 cut(s) 1193, 1392, 1604, 1721, 2382, 2473, 2543, 2597, 2837
PflMI CCANNNNNTGG 2 cut(s) 329, 545
PkrI GCNGC 5 cut(s) 337, 933, 1385, 2133, 2296
PleI GAGTC 7 cut(s) 362, 1115, 1623, 2148, 2400, 2445, 2879
PpsI GAGTC 7 cut(s) 362, 1115, 1623, 2148, 2400, 2445, 2879
PpuMI RGGWCCY 2 cut(s) 527, 887
PshAI GACNNNNGTC 1 cut(s) 2744
PshBI ATTAAT 1 cut(s) 585
PsiI TTATAA 3 cut(s) 983, 1401, 2324
Psp124BI GAGCTC 2 cut(s) 256, 968
Psp1406I AACGTT 1 cut(s) 1069
Psp5II RGGWCCY 2 cut(s) 527, 887
Psp6I CCWGG 4 cut(s) 1387, 1779, 1924, 2673
PspFI CCCAGC 1 cut(s) 2681
PspGI CCWGG 4 cut(s) 1387, 1779, 1924, 2673
PspN4I GGNNCC 2 cut(s) 673, 1183
PspPPI RGGWCCY 2 cut(s) 527, 887
PstI CTGCAG 1 cut(s) 2296
PsuI RGATCY 1 cut(s) 1436
PvuII CAGCTG 1 cut(s) 1081
RsaI GTAC 8 cut(s) 1183, 1597, 1917, 1930, 2243, 2562, 2671, 3151
RsaNI GTAC 8 cut(s) 1182, 1596, 1916, 1929, 2242, 2561, 2670, 3150
RseI CAYNNNNRTG 4 cut(s) 127, 2568, 3017, 3095
SacI GAGCTC 2 cut(s) 256, 968
SapI GCTCTTC 3 cut(s) 462, 973, 2121
SatI GCNGC 5 cut(s) 336, 932, 1384, 2132, 2295
Sau3AI GATC 6 cut(s) 78, 235, 1410, 1436, 2200, 2443
ScaI AGTACT 1 cut(s) 1597
SchI GAGTC 7 cut(s) 362, 1115, 1623, 2148, 2400, 2446, 2879
ScrFI CCNGG 5 cut(s) 1389, 1781, 1926, 2675, 3042
SduI GDGCHC 3 cut(s) 256, 968, 2951
SfaNI GCATC 5 cut(s) 803, 1320, 1829, 2306, 3157
SfcI CTRYAG 3 cut(s) 68, 1218, 2292
SfuI TTCGAA 2 cut(s) 2092, 2554
SinI GGWCC 8 cut(s) 527, 671, 887, 1424, 1481, 1937, 2234, 2484
SmiMI CAYNNNNRTG 4 cut(s) 127, 2568, 3017, 3095
SmlI CTYRAG 4 cut(s) 177, 201, 222, 571
SmoI CTYRAG 4 cut(s) 177, 201, 222, 571
SpeI ACTAGT 1 cut(s) 2878
SsiI CCGC 3 cut(s) 315, 1383, 2784
SspI AATATT 1 cut(s) 2587
SstI GAGCTC 2 cut(s) 256, 968
StyD4I CCNGG 5 cut(s) 1387, 1779, 1924, 2673, 3040
StyI CCWWGG 2 cut(s) 577, 1477
TaaI ACNGT 4 cut(s) 787, 1219, 1687, 2493
TaiI ACGT 5 cut(s) 450, 1041, 1072, 1753, 2869
TaqI TCGA 6 cut(s) 1314, 2092, 2199, 2207, 2554, 2595
TatI WGTACW 4 cut(s) 1595, 2241, 2560, 3149
TauI GCSGC 1 cut(s) 1386
TfiI GAWTC 9 cut(s) 1193, 1392, 1604, 1721, 2382, 2473, 2543, 2597, 2837
TseFI GTSAC 4 cut(s) 1641, 2368, 2798, 3019
TseI GCWGC 4 cut(s) 335, 931, 2131, 2294
Tsp45I GTSAC 4 cut(s) 1641, 2368, 2798, 3019
TspGWI ACGGA 2 cut(s) 100, 3129
Van91I CCANNNNNTGG 2 cut(s) 329, 545
VneI GTGCAC 1 cut(s) 2947
VpaK11BI GGWCC 8 cut(s) 527, 671, 887, 1424, 1481, 1937, 2234, 2484
VspI ATTAAT 1 cut(s) 585
XapI RAATTY 7 cut(s) 36, 259, 502, 694, 806, 1536, 2900
XbaI TCTAGA 2 cut(s) 2476, 2770
XceI RCATGY 2 cut(s) 565, 3150
XcmI CCANNNNNNNNNTGG 3 cut(s) 471, 502, 3094
XmiI GTMKAC 3 cut(s) 1221, 1565, 2788
XmnI GAANNNNTTC 1 cut(s) 3001
ZrmI AGTACT 1 cut(s) 1597
Zsp2I ATGCAT 1 cut(s) 2507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.