LRR receptor-like serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1701 |
| Acc65I | GGTACC | 1 cut(s) | 899 |
| AccB1I | GGYRCC | 2 cut(s) | 209, 899 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 557 |
| AccI | GTMKAC | 2 cut(s) | 508, 934 |
| AccIII | TCCGGA | 1 cut(s) | 536 |
| AciI | CCGC | 2 cut(s) | 177, 1283 |
| AclWI | GGATC | 4 cut(s) | 202, 529, 954, 1507 |
| AcoI | YGGCCR | 1 cut(s) | 448 |
| AcsI | RAATTY | 3 cut(s) | 582, 615, 1222 |
| AcuI | CTGAAG | 3 cut(s) | 126, 1058, 1341 |
| AfaI | GTAC | 3 cut(s) | 5, 259, 901 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 191, 557 |
| AflII | CTTAAG | 1 cut(s) | 494 |
| AjiI | CACGTC | 1 cut(s) | 1298 |
| AjnI | CCWGG | 3 cut(s) | 1198, 1495, 1518 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 429, 461, 757, 789 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 532, 564 |
| Alw21I | GWGCWC | 1 cut(s) | 830 |
| Alw26I | GTCTC | 1 cut(s) | 1559 |
| AlwI | GGATC | 4 cut(s) | 202, 529, 954, 1507 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 943 |
| Ama87I | CYCGRG | 1 cut(s) | 1466 |
| Aor13HI | TCCGGA | 1 cut(s) | 536 |
| AoxI | GGCC | 6 cut(s) | 448, 467, 754, 970, 1374, 1516 |
| ApaI | GGGCCC | 1 cut(s) | 471 |
| ApeKI | GCWGC | 2 cut(s) | 54, 1645 |
| ApoI | RAATTY | 3 cut(s) | 582, 615, 1222 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1069, 1101 |
| AseI | ATTAAT | 1 cut(s) | 890 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 386 |
| Asp718I | GGTACC | 1 cut(s) | 899 |
| AspLEI | GCGC | 1 cut(s) | 1584 |
| AspS9I | GGNCC | 9 cut(s) | 395, 467, 468, 755, 1043, 1280, 1337, 1374, 1535 |
| AsuHPI | GGTGA | 4 cut(s) | 280, 623, 985, 1138 |
| AvaI | CYCGRG | 1 cut(s) | 1466 |
| AvaII | GGWCC | 5 cut(s) | 395, 1043, 1280, 1337, 1535 |
| BaeGI | GKGCMC | 1 cut(s) | 471 |
| BalI | TGGCCA | 1 cut(s) | 450 |
| BanI | GGYRCC | 2 cut(s) | 209, 899 |
| BanII | GRGCYC | 2 cut(s) | 471, 830 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 744, 776 |
| BbsI | GAAGAC | 1 cut(s) | 1329 |
| Bbv12I | GWGCWC | 1 cut(s) | 830 |
| BbvCI | CCTCAGC | 1 cut(s) | 50 |
| BbvI | GCAGC | 2 cut(s) | 41, 1632 |
| BccI | CCATC | 8 cut(s) | 109, 251, 479, 815, 911, 916, 1295, 1397 |
| BceAI | ACGGC | 4 cut(s) | 223, 347, 1361, 1628 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 254, 288 |
| BciT130I | CCWGG | 3 cut(s) | 1200, 1497, 1520 |
| BciVI | GTATCC | 1 cut(s) | 24 |
| BcoDI | GTCTC | 1 cut(s) | 1559 |
| BfaI | CTAG | 1 cut(s) | 1607 |
| BfmI | CTRYAG | 3 cut(s) | 187, 935, 1080 |
| BfrI | CTTAAG | 1 cut(s) | 494 |
| BfuAI | ACCTGC | 1 cut(s) | 1701 |
| BfuI | GTATCC | 1 cut(s) | 24 |
| BisI | GCNGC | 2 cut(s) | 55, 1646 |
| BlsI | GCNGC | 2 cut(s) | 56, 1647 |
| Bme1390I | CCNGG | 3 cut(s) | 1200, 1497, 1520 |
| Bme18I | GGWCC | 5 cut(s) | 395, 1043, 1280, 1337, 1535 |
| BmeT110I | CYCGRG | 1 cut(s) | 1466 |
| BmgBI | CACGTC | 1 cut(s) | 1298 |
| BmgT120I | GGNCC | 9 cut(s) | 395, 467, 468, 755, 1043, 1280, 1337, 1374, 1535 |
| BmiI | GGNNCC | 4 cut(s) | 211, 397, 469, 901 |
| BmrFI | CCNGG | 3 cut(s) | 1200, 1497, 1520 |
| BmrI | ACTGGG | 2 cut(s) | 521, 1114 |
| BmuI | ACTGGG | 2 cut(s) | 521, 1114 |
| BpiI | GAAGAC | 1 cut(s) | 1329 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 817, 849 |
| BpmI | CTGGAG | 3 cut(s) | 534, 560, 1199 |
| Bpu10I | CCTNAGC | 1 cut(s) | 50 |
| BpuEI | CTTGAG | 1 cut(s) | 1547 |
| BsaJI | CCNNGG | 3 cut(s) | 477, 1495, 1519 |
| BsaWI | WCCGGW | 2 cut(s) | 536, 1448 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 518, 532, 548, 562 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 191, 557 |
| Bse118I | RCCGGY | 1 cut(s) | 403 |
| Bse1I | ACTGG | 6 cut(s) | 516, 642, 901, 948, 1120, 1359 |
| Bse3DI | GCAATG | 2 cut(s) | 159, 1390 |
| BseAI | TCCGGA | 1 cut(s) | 536 |
| BseBI | CCWGG | 3 cut(s) | 1200, 1497, 1520 |
| BseDI | CCNNGG | 3 cut(s) | 477, 1495, 1519 |
| BseGI | GGATG | 3 cut(s) | 471, 903, 1010 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 191, 557 |
| BseMI | GCAATG | 2 cut(s) | 159, 1390 |
| BseMII | CTCAG | 5 cut(s) | 64, 287, 693, 941, 1496 |
| BseNI | ACTGG | 6 cut(s) | 516, 642, 901, 948, 1120, 1359 |
| BseRI | GAGGAG | 1 cut(s) | 820 |
| BseSI | GKGCMC | 1 cut(s) | 471 |
| BseXI | GCAGC | 2 cut(s) | 41, 1632 |
| BseYI | CCCAGC | 1 cut(s) | 238 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1418 |
| BshFI | GGCC | 6 cut(s) | 450, 469, 756, 972, 1376, 1518 |
| BshNI | GGYRCC | 2 cut(s) | 209, 899 |
| BsiEI | CGRYCG | 1 cut(s) | 1418 |
| BsiHKAI | GWGCWC | 1 cut(s) | 830 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1466 |
| BsiSI | CCGG | 3 cut(s) | 404, 537, 1449 |
| BslFI | GGGAC | 4 cut(s) | 155, 381, 1308, 1477 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 191, 557 |
| BsmAI | GTCTC | 1 cut(s) | 1559 |
| BsmBI | CGTCTC | 1 cut(s) | 1559 |
| BsmFI | GGGAC | 4 cut(s) | 155, 381, 1308, 1477 |
| BsnI | GGCC | 6 cut(s) | 450, 469, 756, 972, 1376, 1518 |
| BsoBI | CYCGRG | 1 cut(s) | 1466 |
| Bsp120I | GGGCCC | 1 cut(s) | 467 |
| Bsp1286I | GDGCHC | 2 cut(s) | 471, 830 |
| Bsp13I | TCCGGA | 1 cut(s) | 536 |
| Bsp1407I | TGTACA | 1 cut(s) | 3 |
| Bsp143I | GATC | 5 cut(s) | 194, 521, 946, 1415, 1499 |
| BspACI | CCGC | 2 cut(s) | 177, 1283 |
| BspANI | GGCC | 6 cut(s) | 450, 469, 756, 972, 1376, 1518 |
| BspCNI | CTCAG | 5 cut(s) | 63, 288, 692, 942, 1497 |
| BspEI | TCCGGA | 1 cut(s) | 536 |
| BspHI | TCATGA | 1 cut(s) | 331 |
| BspLI | GGNNCC | 4 cut(s) | 211, 397, 469, 901 |
| BspMAI | CTGCAG | 1 cut(s) | 191 |
| BspMI | ACCTGC | 1 cut(s) | 1701 |
| BspPI | GGATC | 4 cut(s) | 202, 529, 954, 1507 |
| BspT107I | GGYRCC | 2 cut(s) | 209, 899 |
| BspTI | CTTAAG | 1 cut(s) | 494 |
| BsrDI | GCAATG | 2 cut(s) | 159, 1390 |
| BsrFI | RCCGGY | 1 cut(s) | 403 |
| BsrGI | TGTACA | 1 cut(s) | 3 |
| BsrI | ACTGG | 6 cut(s) | 516, 642, 901, 948, 1120, 1359 |
| BssAI | RCCGGY | 1 cut(s) | 403 |
| BssECI | CCNNGG | 3 cut(s) | 477, 1495, 1519 |
| BssMI | GATC | 5 cut(s) | 194, 521, 946, 1415, 1499 |
| BssNAI | GTATAC | 1 cut(s) | 509 |
| BssT1I | CCWWGG | 1 cut(s) | 477 |
| Bst1107I | GTATAC | 1 cut(s) | 509 |
| Bst2UI | CCWGG | 3 cut(s) | 1200, 1497, 1520 |
| Bst4CI | ACNGT | 4 cut(s) | 257, 671, 1546, 1570 |
| Bst6I | CTCTTC | 2 cut(s) | 1053, 1200 |
| BstAFI | CTTAAG | 1 cut(s) | 494 |
| BstAUI | TGTACA | 1 cut(s) | 3 |
| BstC8I | GCNNGC | 1 cut(s) | 306 |
| BstDEI | CTNAG | 8 cut(s) | 50, 296, 350, 503, 679, 950, 1150, 1505 |
| BstEII | GGTNACC | 1 cut(s) | 1126 |
| BstF5I | GGATG | 3 cut(s) | 471, 903, 1010 |
| BstHHI | GCGC | 1 cut(s) | 1584 |
| BstKTI | GATC | 5 cut(s) | 197, 524, 949, 1418, 1502 |
| BstMAI | GTCTC | 1 cut(s) | 1559 |
| BstMBI | GATC | 5 cut(s) | 194, 521, 946, 1415, 1499 |
| BstMCI | CGRYCG | 1 cut(s) | 1418 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 51, 1062, 1588 |
| BstNI | CCWGG | 3 cut(s) | 1200, 1497, 1520 |
| BstNSI | RCATGY | 1 cut(s) | 282 |
| BstPI | GGTNACC | 1 cut(s) | 1126 |
| BstSCI | CCNGG | 3 cut(s) | 1198, 1495, 1518 |
| BstSFI | CTRYAG | 3 cut(s) | 187, 935, 1080 |
| BstSLI | GKGCMC | 1 cut(s) | 471 |
| BstV1I | GCAGC | 2 cut(s) | 41, 1632 |
| BstV2I | GAAGAC | 1 cut(s) | 1329 |
| BstX2I | RGATCY | 2 cut(s) | 194, 946 |
| BstYI | RGATCY | 2 cut(s) | 194, 946 |
| BstZ17I | GTATAC | 1 cut(s) | 509 |
| BsuI | GTATCC | 1 cut(s) | 24 |
| BsuRI | GGCC | 6 cut(s) | 450, 469, 756, 972, 1376, 1518 |
| BtrI | CACGTC | 1 cut(s) | 1298 |
| BtsCI | GGATG | 3 cut(s) | 471, 903, 1010 |
| BtsI | GCAGTG | 1 cut(s) | 196 |
| BtsIMutI | CAGTG | 8 cut(s) | 93, 196, 613, 814, 894, 1127, 1502, 1542 |
| BveI | ACCTGC | 1 cut(s) | 1701 |
| Cac8I | GCNNGC | 1 cut(s) | 306 |
| CaiI | CAGNNNCTG | 1 cut(s) | 943 |
| CciI | TCATGA | 1 cut(s) | 331 |
| CfoI | GCGC | 1 cut(s) | 1584 |
| Cfr10I | RCCGGY | 1 cut(s) | 403 |
| Cfr13I | GGNCC | 9 cut(s) | 395, 467, 468, 755, 1043, 1280, 1337, 1374, 1535 |
| Csp6I | GTAC | 3 cut(s) | 4, 258, 900 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1258, 1293 |
| CviAII | CATG | 5 cut(s) | 279, 332, 589, 997, 1230 |
| CviQI | GTAC | 3 cut(s) | 4, 258, 900 |
| DdeI | CTNAG | 8 cut(s) | 50, 296, 350, 503, 679, 950, 1150, 1505 |
| DpnI | GATC | 5 cut(s) | 196, 523, 948, 1417, 1501 |
| DpnII | GATC | 5 cut(s) | 194, 521, 946, 1415, 1499 |
| EaeI | YGGCCR | 1 cut(s) | 448 |
| Eam1104I | CTCTTC | 2 cut(s) | 1053, 1200 |
| EarI | CTCTTC | 2 cut(s) | 1053, 1200 |
| Ecl136II | GAGCTC | 1 cut(s) | 828 |
| Eco130I | CCWWGG | 1 cut(s) | 477 |
| Eco24I | GRGCYC | 2 cut(s) | 471, 830 |
| Eco32I | GATATC | 2 cut(s) | 356, 1627 |
| Eco47I | GGWCC | 5 cut(s) | 395, 1043, 1280, 1337, 1535 |
| Eco53kI | GAGCTC | 1 cut(s) | 828 |
| Eco57I | CTGAAG | 3 cut(s) | 126, 1058, 1341 |
| Eco88I | CYCGRG | 1 cut(s) | 1466 |
| Eco91I | GGTNACC | 1 cut(s) | 1126 |
| EcoICRI | GAGCTC | 1 cut(s) | 828 |
| EcoO109I | RGGNCCY | 1 cut(s) | 395 |
| EcoO65I | GGTNACC | 1 cut(s) | 1126 |
| EcoRII | CCWGG | 3 cut(s) | 1198, 1495, 1518 |
| EcoRV | GATATC | 2 cut(s) | 356, 1627 |
| EcoT14I | CCWWGG | 1 cut(s) | 477 |
| EcoT22I | ATGCAT | 1 cut(s) | 889 |
| EcoT38I | GRGCYC | 2 cut(s) | 471, 830 |
| ErhI | CCWWGG | 1 cut(s) | 477 |
| Esp3I | CGTCTC | 1 cut(s) | 1559 |
| FaeI | CATG | 5 cut(s) | 282, 335, 592, 1000, 1233 |
| FaqI | GGGAC | 4 cut(s) | 155, 381, 1308, 1477 |
| FatI | CATG | 5 cut(s) | 278, 331, 588, 996, 1229 |
| FblI | GTMKAC | 2 cut(s) | 508, 934 |
| Fnu4HI | GCNGC | 2 cut(s) | 55, 1646 |
| FokI | GGATG | 3 cut(s) | 458, 890, 997 |
| FriOI | GRGCYC | 2 cut(s) | 471, 830 |
| Fsp4HI | GCNGC | 2 cut(s) | 55, 1646 |
| FspBI | CTAG | 1 cut(s) | 1607 |
| GlaI | GCGC | 1 cut(s) | 1583 |
| GluI | GCNGC | 2 cut(s) | 55, 1646 |
| GsaI | CCCAGC | 1 cut(s) | 242 |
| GsuI | CTGGAG | 3 cut(s) | 534, 560, 1199 |
| HaeIII | GGCC | 6 cut(s) | 450, 469, 756, 972, 1376, 1518 |
| HapII | CCGG | 3 cut(s) | 404, 537, 1449 |
| HhaI | GCGC | 1 cut(s) | 1584 |
| Hin1II | CATG | 5 cut(s) | 282, 335, 592, 1000, 1233 |
| Hin6I | GCGC | 1 cut(s) | 1582 |
| HinP1I | GCGC | 1 cut(s) | 1582 |
| HincII | GTYRAC | 1 cut(s) | 1159 |
| HindII | GTYRAC | 1 cut(s) | 1159 |
| HindIII | AAGCTT | 1 cut(s) | 1111 |
| HinfI | GANTC | 3 cut(s) | 603, 1325, 1700 |
| HpaII | CCGG | 3 cut(s) | 404, 537, 1449 |
| HphI | GGTGA | 4 cut(s) | 280, 623, 985, 1138 |
| Hpy166II | GTNNAC | 6 cut(s) | 509, 935, 993, 1159, 1280, 1411 |
| Hpy188I | TCNGA | 8 cut(s) | 106, 521, 667, 913, 951, 1053, 1077, 1402 |
| Hpy8I | GTNNAC | 6 cut(s) | 509, 935, 993, 1159, 1280, 1411 |
| HpyAV | CCTTC | 4 cut(s) | 211, 630, 746, 1285 |
| HpyCH4III | ACNGT | 4 cut(s) | 257, 671, 1546, 1570 |
| HpyCH4IV | ACGT | 1 cut(s) | 1297 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 51, 1062, 1588 |
| HpyF3I | CTNAG | 8 cut(s) | 50, 296, 350, 503, 679, 950, 1150, 1505 |
| HpySE526I | ACGT | 1 cut(s) | 1297 |
| Hsp92II | CATG | 5 cut(s) | 282, 335, 592, 1000, 1233 |
| HspAI | GCGC | 1 cut(s) | 1582 |
| Kpn2I | TCCGGA | 1 cut(s) | 536 |
| KpnI | GGTACC | 1 cut(s) | 903 |
| Kzo9I | GATC | 5 cut(s) | 194, 521, 946, 1415, 1499 |
| LmnI | GCTCC | 5 cut(s) | 481, 499, 579, 825, 833 |
| Lsp1109I | GCAGC | 2 cut(s) | 41, 1632 |
| MaeI | CTAG | 1 cut(s) | 1607 |
| MaeII | ACGT | 1 cut(s) | 1297 |
| MaeIII | GTNAC | 4 cut(s) | 1126, 1354, 1444, 1459 |
| MalI | GATC | 5 cut(s) | 196, 523, 948, 1417, 1501 |
| MboI | GATC | 5 cut(s) | 194, 521, 946, 1415, 1499 |
| MboII | GAAGA | 7 cut(s) | 25, 347, 868, 1040, 1187, 1334, 1451 |
| MfeI | CAATTG | 1 cut(s) | 1270 |
| MflI | RGATCY | 2 cut(s) | 194, 946 |
| MhlI | GDGCHC | 2 cut(s) | 471, 830 |
| MlsI | TGGCCA | 1 cut(s) | 450 |
| MluNI | TGGCCA | 1 cut(s) | 450 |
| MlyI | GAGTC | 3 cut(s) | 612, 1319, 1694 |
| Mox20I | TGGCCA | 1 cut(s) | 450 |
| Mph1103I | ATGCAT | 1 cut(s) | 889 |
| MroI | TCCGGA | 1 cut(s) | 536 |
| MroXI | GAANNNNTTC | 1 cut(s) | 386 |
| MscI | TGGCCA | 1 cut(s) | 450 |
| MseI | TTAA | 4 cut(s) | 495, 872, 890, 1726 |
| MslI | CAYNNNNRTG | 1 cut(s) | 807 |
| Msp20I | TGGCCA | 1 cut(s) | 450 |
| MspA1I | CMGCKG | 4 cut(s) | 54, 70, 177, 597 |
| MspCI | CTTAAG | 1 cut(s) | 494 |
| MspI | CCGG | 3 cut(s) | 404, 537, 1449 |
| MspR9I | CCNGG | 3 cut(s) | 1200, 1497, 1520 |
| MunI | CAATTG | 1 cut(s) | 1270 |
| MvaI | CCWGG | 3 cut(s) | 1200, 1497, 1520 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 51, 1062, 1588 |
| NdeII | GATC | 5 cut(s) | 194, 521, 946, 1415, 1499 |
| NlaIII | CATG | 5 cut(s) | 282, 335, 592, 1000, 1233 |
| NlaIV | GGNNCC | 4 cut(s) | 211, 397, 469, 901 |
| NmuCI | GTSAC | 2 cut(s) | 1126, 1459 |
| NsiI | ATGCAT | 1 cut(s) | 889 |
| NspI | RCATGY | 1 cut(s) | 282 |
| PagI | TCATGA | 1 cut(s) | 331 |
| PdmI | GAANNNNTTC | 1 cut(s) | 386 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 557 |
| PfoI | TCCNGGA | 1 cut(s) | 1198 |
| PkrI | GCNGC | 2 cut(s) | 56, 1647 |
| Ple19I | CGATCG | 1 cut(s) | 1418 |
| PleI | GAGTC | 3 cut(s) | 611, 1319, 1694 |
| PpsI | GAGTC | 3 cut(s) | 611, 1319, 1694 |
| PpuMI | RGGWCCY | 1 cut(s) | 395 |
| PshBI | ATTAAT | 1 cut(s) | 890 |
| Psp124BI | GAGCTC | 1 cut(s) | 830 |
| Psp5II | RGGWCCY | 1 cut(s) | 395 |
| Psp6I | CCWGG | 3 cut(s) | 1198, 1495, 1518 |
| PspEI | GGTNACC | 1 cut(s) | 1126 |
| PspFI | CCCAGC | 1 cut(s) | 238 |
| PspGI | CCWGG | 3 cut(s) | 1198, 1495, 1518 |
| PspN4I | GGNNCC | 4 cut(s) | 211, 397, 469, 901 |
| PspOMI | GGGCCC | 1 cut(s) | 467 |
| PspPI | GGNCC | 9 cut(s) | 395, 467, 468, 755, 1043, 1280, 1337, 1374, 1535 |
| PspPPI | RGGWCCY | 1 cut(s) | 395 |
| PstI | CTGCAG | 1 cut(s) | 191 |
| PstNI | CAGNNNCTG | 1 cut(s) | 943 |
| PsuI | RGATCY | 2 cut(s) | 194, 946 |
| PvuI | CGATCG | 1 cut(s) | 1418 |
| PvuII | CAGCTG | 3 cut(s) | 54, 70, 597 |
| RsaI | GTAC | 3 cut(s) | 5, 259, 901 |
| RsaNI | GTAC | 3 cut(s) | 4, 258, 900 |
| RseI | CAYNNNNRTG | 1 cut(s) | 807 |
| SacI | GAGCTC | 1 cut(s) | 830 |
| SaqAI | TTAA | 4 cut(s) | 495, 872, 890, 1726 |
| SatI | GCNGC | 2 cut(s) | 55, 1646 |
| Sau3AI | GATC | 5 cut(s) | 194, 521, 946, 1415, 1499 |
| Sau96I | GGNCC | 9 cut(s) | 395, 467, 468, 755, 1043, 1280, 1337, 1374, 1535 |
| SchI | GAGTC | 3 cut(s) | 612, 1319, 1694 |
| ScrFI | CCNGG | 3 cut(s) | 1200, 1497, 1520 |
| SduI | GDGCHC | 2 cut(s) | 471, 830 |
| SfcI | CTRYAG | 3 cut(s) | 187, 935, 1080 |
| SinI | GGWCC | 5 cut(s) | 395, 1043, 1280, 1337, 1535 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 807 |
| SmlI | CTYRAG | 2 cut(s) | 494, 1526 |
| SmoI | CTYRAG | 2 cut(s) | 494, 1526 |
| SsiI | CCGC | 2 cut(s) | 177, 1283 |
| SspI | AATATT | 1 cut(s) | 129 |
| SspMI | CTAG | 1 cut(s) | 1607 |
| SstI | GAGCTC | 1 cut(s) | 830 |
| StyD4I | CCNGG | 3 cut(s) | 1198, 1495, 1518 |
| StyI | CCWWGG | 1 cut(s) | 477 |
| TaaI | ACNGT | 4 cut(s) | 257, 671, 1546, 1570 |
| TaiI | ACGT | 1 cut(s) | 1300 |
| TaqI | TCGA | 2 cut(s) | 1237, 1718 |
| TatI | WGTACW | 2 cut(s) | 3, 257 |
| Tru1I | TTAA | 4 cut(s) | 495, 872, 890, 1726 |
| Tru9I | TTAA | 4 cut(s) | 495, 872, 890, 1726 |
| TscAI | CASTG | 8 cut(s) | 100, 196, 613, 814, 901, 1127, 1509, 1549 |
| TseFI | GTSAC | 2 cut(s) | 1126, 1459 |
| TseI | GCWGC | 2 cut(s) | 54, 1645 |
| Tsp45I | GTSAC | 2 cut(s) | 1126, 1459 |
| TspDTI | ATGAA | 9 cut(s) | 26, 32, 320, 348, 447, 688, 832, 869, 1304 |
| TspRI | CASTG | 8 cut(s) | 100, 196, 613, 814, 901, 1127, 1509, 1549 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 557 |
| Vha464I | CTTAAG | 1 cut(s) | 494 |
| VpaK11BI | GGWCC | 5 cut(s) | 395, 1043, 1280, 1337, 1535 |
| VspI | ATTAAT | 1 cut(s) | 890 |
| XapI | RAATTY | 3 cut(s) | 582, 615, 1222 |
| XceI | RCATGY | 1 cut(s) | 282 |
| XmiI | GTMKAC | 2 cut(s) | 508, 934 |
| XmnI | GAANNNNTTC | 1 cut(s) | 386 |
| XspI | CTAG | 1 cut(s) | 1607 |
| Zsp2I | ATGCAT | 1 cut(s) | 889 |