Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 98 |
| AfaI | GTAC | 2 cut(s) | 115, 220 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 28 |
| AgsI | TTSAA | 2 cut(s) | 72, 202 |
| AluBI | AGCT | 1 cut(s) | 198 |
| AluI | AGCT | 1 cut(s) | 198 |
| ApoI | RAATTY | 1 cut(s) | 98 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 83, 115 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 37 |
| AsuHPI | GGTGA | 3 cut(s) | 112, 148, 231 |
| BccI | CCATC | 1 cut(s) | 19 |
| BfaI | CTAG | 1 cut(s) | 180 |
| BmrI | ACTGGG | 1 cut(s) | 72 |
| BmuI | ACTGGG | 1 cut(s) | 72 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 28 |
| Bse1I | ACTGG | 3 cut(s) | 78, 98, 141 |
| BseGI | GGATG | 1 cut(s) | 11 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 28 |
| BseNI | ACTGG | 3 cut(s) | 78, 98, 141 |
| BslFI | GGGAC | 2 cut(s) | 4, 178 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 28 |
| BsmFI | GGGAC | 2 cut(s) | 4, 178 |
| BsmI | GAATGC | 1 cut(s) | 39 |
| BsrI | ACTGG | 3 cut(s) | 78, 98, 141 |
| Bst4CI | ACNGT | 3 cut(s) | 64, 118, 143 |
| BstF5I | GGATG | 1 cut(s) | 11 |
| BtsCI | GGATG | 1 cut(s) | 11 |
| Csp6I | GTAC | 2 cut(s) | 114, 219 |
| CviAII | CATG | 1 cut(s) | 16 |
| CviJI | RGCY | 3 cut(s) | 76, 126, 198 |
| CviKI_1 | RGCY | 3 cut(s) | 76, 126, 198 |
| CviQI | GTAC | 2 cut(s) | 114, 219 |
| FaeI | CATG | 1 cut(s) | 19 |
| FaiI | YATR | 5 cut(s) | 17, 50, 173, 227, 263 |
| FaqI | GGGAC | 2 cut(s) | 4, 178 |
| FatI | CATG | 1 cut(s) | 15 |
| FspBI | CTAG | 1 cut(s) | 180 |
| Hin1II | CATG | 1 cut(s) | 19 |
| HphI | GGTGA | 3 cut(s) | 112, 148, 231 |
| Hpy188III | TCNNGA | 1 cut(s) | 42 |
| HpyCH4III | ACNGT | 3 cut(s) | 64, 118, 143 |
| Hsp92II | CATG | 1 cut(s) | 19 |
| LpnPI | CCDG | 5 cut(s) | 27, 79, 91, 122, 136 |
| MaeI | CTAG | 1 cut(s) | 180 |
| MaeIII | GTNAC | 5 cut(s) | 55, 64, 80, 118, 154 |
| MluCI | AATT | 1 cut(s) | 98 |
| MnlI | CCTC | 2 cut(s) | 176, 252 |
| MroXI | GAANNNNTTC | 1 cut(s) | 37 |
| MseI | TTAA | 1 cut(s) | 110 |
| Mva1269I | GAATGC | 1 cut(s) | 39 |
| NlaIII | CATG | 1 cut(s) | 19 |
| NmuCI | GTSAC | 3 cut(s) | 55, 118, 154 |
| PctI | GAATGC | 1 cut(s) | 39 |
| PdmI | GAANNNNTTC | 1 cut(s) | 37 |
| RsaI | GTAC | 2 cut(s) | 115, 220 |
| RsaNI | GTAC | 2 cut(s) | 114, 219 |
| SaqAI | TTAA | 1 cut(s) | 110 |
| SetI | ASST | 2 cut(s) | 200, 244 |
| Sse9I | AATT | 1 cut(s) | 98 |
| SspMI | CTAG | 1 cut(s) | 180 |
| TaaI | ACNGT | 3 cut(s) | 64, 118, 143 |
| TasI | AATT | 1 cut(s) | 98 |
| TatI | WGTACW | 2 cut(s) | 113, 218 |
| Tru1I | TTAA | 1 cut(s) | 110 |
| Tru9I | TTAA | 1 cut(s) | 110 |
| TseFI | GTSAC | 3 cut(s) | 55, 118, 154 |
| Tsp45I | GTSAC | 3 cut(s) | 55, 118, 154 |
| TspDTI | ATGAA | 1 cut(s) | 245 |
| XapI | RAATTY | 1 cut(s) | 98 |
| XmnI | GAANNNNTTC | 1 cut(s) | 37 |
| XspI | CTAG | 1 cut(s) | 180 |