Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 43 |
| AccB1I | GGYRCC | 1 cut(s) | 458 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 368 |
| AcsI | RAATTY | 2 cut(s) | 298, 401 |
| AcuI | CTGAAG | 1 cut(s) | 370 |
| AfaI | GTAC | 1 cut(s) | 57 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 368 |
| AgsI | TTSAA | 1 cut(s) | 298 |
| AjnI | CCWGG | 1 cut(s) | 96 |
| AluBI | AGCT | 4 cut(s) | 224, 293, 374, 481 |
| AluI | AGCT | 4 cut(s) | 224, 293, 374, 481 |
| Alw26I | GTCTC | 1 cut(s) | 351 |
| AoxI | GGCC | 1 cut(s) | 327 |
| ApeKI | GCWGC | 1 cut(s) | 374 |
| ApoI | RAATTY | 2 cut(s) | 298, 401 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 155 |
| AspS9I | GGNCC | 1 cut(s) | 327 |
| AsuHPI | GGTGA | 1 cut(s) | 85 |
| BanI | GGYRCC | 1 cut(s) | 458 |
| BanII | GRGCYC | 1 cut(s) | 38 |
| BbsI | GAAGAC | 1 cut(s) | 5 |
| BbvI | GCAGC | 1 cut(s) | 361 |
| BccI | CCATC | 2 cut(s) | 187, 286 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 62, 96 |
| BciT130I | CCWGG | 1 cut(s) | 98 |
| BclI | TGATCA | 1 cut(s) | 90 |
| BcoDI | GTCTC | 1 cut(s) | 351 |
| BfuAI | ACCTGC | 1 cut(s) | 43 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 42 |
| BisI | GCNGC | 1 cut(s) | 375 |
| BlsI | GCNGC | 1 cut(s) | 376 |
| Bme1390I | CCNGG | 1 cut(s) | 98 |
| BmgT120I | GGNCC | 1 cut(s) | 327 |
| BmiI | GGNNCC | 1 cut(s) | 460 |
| BmrFI | CCNGG | 1 cut(s) | 98 |
| BmrI | ACTGGG | 1 cut(s) | 104 |
| BmsI | GCATC | 1 cut(s) | 213 |
| BmuI | ACTGGG | 1 cut(s) | 104 |
| BoxI | GACNNNNGTC | 1 cut(s) | 387 |
| BpiI | GAAGAC | 1 cut(s) | 5 |
| BpmI | CTGGAG | 1 cut(s) | 390 |
| BpuEI | CTTGAG | 4 cut(s) | 50, 210, 237, 267 |
| BsaI | GGTCTC | 1 cut(s) | 351 |
| BsaJI | CCNNGG | 1 cut(s) | 96 |
| BsaWI | WCCGGW | 1 cut(s) | 353 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 368 |
| Bse1I | ACTGG | 2 cut(s) | 110, 373 |
| Bse3DI | GCAATG | 1 cut(s) | 347 |
| BseBI | CCWGG | 1 cut(s) | 98 |
| BseDI | CCNNGG | 1 cut(s) | 96 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 368 |
| BseMI | GCAATG | 1 cut(s) | 347 |
| BseMII | CTCAG | 1 cut(s) | 141 |
| BseNI | ACTGG | 2 cut(s) | 110, 373 |
| BseRI | GAGGAG | 1 cut(s) | 234 |
| BseXI | GCAGC | 1 cut(s) | 361 |
| BseYI | CCCAGC | 1 cut(s) | 477 |
| BsgI | GTGCAG | 1 cut(s) | 71 |
| BshFI | GGCC | 1 cut(s) | 329 |
| BshNI | GGYRCC | 1 cut(s) | 458 |
| BsiSI | CCGG | 2 cut(s) | 354, 456 |
| BslFI | GGGAC | 1 cut(s) | 108 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 368 |
| BsmAI | GTCTC | 1 cut(s) | 351 |
| BsmFI | GGGAC | 1 cut(s) | 108 |
| BsmI | GAATGC | 1 cut(s) | 155 |
| BsnI | GGCC | 1 cut(s) | 329 |
| Bso31I | GGTCTC | 1 cut(s) | 351 |
| Bsp1286I | GDGCHC | 1 cut(s) | 38 |
| Bsp143I | GATC | 2 cut(s) | 60, 90 |
| BspANI | GGCC | 1 cut(s) | 329 |
| BspCNI | CTCAG | 1 cut(s) | 140 |
| BspLI | GGNNCC | 1 cut(s) | 460 |
| BspMI | ACCTGC | 1 cut(s) | 43 |
| BspT107I | GGYRCC | 1 cut(s) | 458 |
| BspTNI | GGTCTC | 1 cut(s) | 351 |
| BsrDI | GCAATG | 1 cut(s) | 347 |
| BsrI | ACTGG | 2 cut(s) | 110, 373 |
| BssECI | CCNNGG | 1 cut(s) | 96 |
| BssMI | GATC | 2 cut(s) | 60, 90 |
| Bst2UI | CCWGG | 1 cut(s) | 98 |
| Bst4CI | ACNGT | 1 cut(s) | 120 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 465 |
| BstDEI | CTNAG | 1 cut(s) | 127 |
| BstKTI | GATC | 2 cut(s) | 63, 93 |
| BstMAI | GTCTC | 1 cut(s) | 351 |
| BstMBI | GATC | 2 cut(s) | 60, 90 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 42, 263, 465 |
| BstNI | CCWGG | 1 cut(s) | 98 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 387 |
| BstSCI | CCNGG | 1 cut(s) | 96 |
| BstV1I | GCAGC | 1 cut(s) | 361 |
| BstV2I | GAAGAC | 1 cut(s) | 5 |
| BsuRI | GGCC | 1 cut(s) | 329 |
| BtsIMutI | CAGTG | 4 cut(s) | 116, 270, 309, 366 |
| BveI | ACCTGC | 1 cut(s) | 43 |
| Cfr13I | GGNCC | 1 cut(s) | 327 |
| Csp6I | GTAC | 1 cut(s) | 56 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 385, 420 |
| CviAII | CATG | 3 cut(s) | 136, 160, 363 |
| CviJI | RGCY | 9 cut(s) | 36, 45, 101, 224, 257, 293, 329, 374, 481 |
| CviKI_1 | RGCY | 9 cut(s) | 36, 45, 101, 224, 257, 293, 329, 374, 481 |
| CviQI | GTAC | 1 cut(s) | 56 |
| DdeI | CTNAG | 1 cut(s) | 127 |
| DpnI | GATC | 2 cut(s) | 62, 92 |
| DpnII | GATC | 2 cut(s) | 60, 90 |
| Eco24I | GRGCYC | 1 cut(s) | 38 |
| Eco31I | GGTCTC | 1 cut(s) | 351 |
| Eco57I | CTGAAG | 1 cut(s) | 370 |
| EcoRI | GAATTC | 1 cut(s) | 298 |
| EcoRII | CCWGG | 1 cut(s) | 96 |
| EcoT38I | GRGCYC | 1 cut(s) | 38 |
| FaeI | CATG | 3 cut(s) | 139, 163, 366 |
| FaiI | YATR | 5 cut(s) | 137, 161, 215, 364, 380 |
| FaqI | GGGAC | 1 cut(s) | 108 |
| FatI | CATG | 3 cut(s) | 135, 159, 362 |
| FbaI | TGATCA | 1 cut(s) | 90 |
| Fnu4HI | GCNGC | 1 cut(s) | 375 |
| FriOI | GRGCYC | 1 cut(s) | 38 |
| Fsp4HI | GCNGC | 1 cut(s) | 375 |
| GluI | GCNGC | 1 cut(s) | 375 |
| GsaI | CCCAGC | 1 cut(s) | 481 |
| GsuI | CTGGAG | 1 cut(s) | 390 |
| HaeIII | GGCC | 1 cut(s) | 329 |
| HapII | CCGG | 2 cut(s) | 354, 456 |
| Hin1II | CATG | 3 cut(s) | 139, 163, 366 |
| HincII | GTYRAC | 1 cut(s) | 141 |
| HindII | GTYRAC | 1 cut(s) | 141 |
| HinfI | GANTC | 1 cut(s) | 74 |
| HpaII | CCGG | 2 cut(s) | 354, 456 |
| HphI | GGTGA | 1 cut(s) | 85 |
| Hpy166II | GTNNAC | 2 cut(s) | 141, 418 |
| Hpy188I | TCNGA | 2 cut(s) | 26, 336 |
| Hpy8I | GTNNAC | 2 cut(s) | 141, 418 |
| HpyAV | CCTTC | 1 cut(s) | 436 |
| HpyCH4III | ACNGT | 1 cut(s) | 120 |
| HpyCH4V | TGCA | 3 cut(s) | 52, 204, 366 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 42, 263, 465 |
| HpyF3I | CTNAG | 1 cut(s) | 127 |
| Hsp92II | CATG | 3 cut(s) | 139, 163, 366 |
| Ksp22I | TGATCA | 1 cut(s) | 90 |
| Kzo9I | GATC | 2 cut(s) | 60, 90 |
| LmnI | GCTCC | 3 cut(s) | 241, 247, 371 |
| Lsp1109I | GCAGC | 1 cut(s) | 361 |
| LweI | GCATC | 1 cut(s) | 213 |
| MaeIII | GTNAC | 2 cut(s) | 3, 389 |
| MalI | GATC | 2 cut(s) | 62, 92 |
| MboI | GATC | 2 cut(s) | 60, 90 |
| MboII | GAAGA | 1 cut(s) | 5 |
| MhlI | GDGCHC | 1 cut(s) | 38 |
| MluCI | AATT | 2 cut(s) | 298, 401 |
| MlyI | GAGTC | 1 cut(s) | 68 |
| MnlI | CCTC | 5 cut(s) | 18, 136, 184, 255, 261 |
| MroXI | GAANNNNTTC | 1 cut(s) | 155 |
| MspA1I | CMGCKG | 1 cut(s) | 481 |
| MspI | CCGG | 2 cut(s) | 354, 456 |
| MspR9I | CCNGG | 1 cut(s) | 98 |
| Mva1269I | GAATGC | 1 cut(s) | 155 |
| MvaI | CCWGG | 1 cut(s) | 98 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 42, 263, 465 |
| NdeII | GATC | 2 cut(s) | 60, 90 |
| NlaIII | CATG | 3 cut(s) | 139, 163, 366 |
| NlaIV | GGNNCC | 1 cut(s) | 460 |
| NmuCI | GTSAC | 2 cut(s) | 3, 389 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 69 |
| PctI | GAATGC | 1 cut(s) | 155 |
| PdmI | GAANNNNTTC | 1 cut(s) | 155 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 368 |
| PkrI | GCNGC | 1 cut(s) | 376 |
| PleI | GAGTC | 1 cut(s) | 68 |
| PpsI | GAGTC | 1 cut(s) | 68 |
| PshAI | GACNNNNGTC | 1 cut(s) | 387 |
| Psp6I | CCWGG | 1 cut(s) | 96 |
| PspFI | CCCAGC | 1 cut(s) | 477 |
| PspGI | CCWGG | 1 cut(s) | 96 |
| PspN4I | GGNNCC | 1 cut(s) | 460 |
| PspPI | GGNCC | 1 cut(s) | 327 |
| PvuII | CAGCTG | 1 cut(s) | 481 |
| RsaI | GTAC | 1 cut(s) | 57 |
| RsaNI | GTAC | 1 cut(s) | 56 |
| SatI | GCNGC | 1 cut(s) | 375 |
| Sau3AI | GATC | 2 cut(s) | 60, 90 |
| Sau96I | GGNCC | 1 cut(s) | 327 |
| SchI | GAGTC | 1 cut(s) | 68 |
| ScrFI | CCNGG | 1 cut(s) | 98 |
| SduI | GDGCHC | 1 cut(s) | 38 |
| SetI | ASST | 7 cut(s) | 10, 57, 226, 253, 295, 376, 483 |
| SfaNI | GCATC | 1 cut(s) | 213 |
| SmlI | CTYRAG | 4 cut(s) | 29, 225, 252, 282 |
| SmoI | CTYRAG | 4 cut(s) | 29, 225, 252, 282 |
| Sse9I | AATT | 2 cut(s) | 298, 401 |
| SspI | AATATT | 1 cut(s) | 322 |
| StyD4I | CCNGG | 1 cut(s) | 96 |
| TaaI | ACNGT | 1 cut(s) | 120 |
| TaqI | TCGA | 1 cut(s) | 72 |
| TasI | AATT | 2 cut(s) | 298, 401 |
| TscAI | CASTG | 4 cut(s) | 123, 277, 309, 373 |
| TseFI | GTSAC | 2 cut(s) | 3, 389 |
| TseI | GCWGC | 1 cut(s) | 374 |
| Tsp45I | GTSAC | 2 cut(s) | 3, 389 |
| TspDTI | ATGAA | 3 cut(s) | 148, 164, 418 |
| TspRI | CASTG | 4 cut(s) | 123, 277, 309, 373 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 368 |
| XapI | RAATTY | 2 cut(s) | 298, 401 |
| XmnI | GAANNNNTTC | 1 cut(s) | 155 |