RchiOBHm_Chr5g0073701
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000088
Physical Location & Seq
Forward (+)
79760050 .. 79770131
10082 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34858

Sequence Viewer

Length: 3177 bp
ATGTCACTGGTGGTGTGGTTTTGGTATGCTGCAGCAGCCTGCAGCATATTCATCTCTGGGTTTGTTGCACAGGCTCAACCCACCACAGATACCTCTGAAGTGAGAGCGCTGAATGCAATCTTTCAGAAATGGAATATTACGGTCAATACAAAGCAATGGAACACAACTGGTGATCCATGCAGTGAACTTGCGGTAGATGAAGATGATGAGACCGCTTATTTCCATGACGATGATTACAATCTCCTCGTCAAATGCAACTGCTCTTATAATTCAAACTCAACTTGCCACATTACCCAACTGAAAGCAATCACTTTGGACATTGTTGCTCCAATTCCAGAAGAGCTCTATGCTTTGACTTTCCTTCAATATCTGTATGTTCGGAATGTGAAAAAAAATTACTTGACAGGACCTATCTCTGCATCGATTGGAAATCTAACCAGCATCAAATACTTGGACTTGGGACACAATGACTTATCAGGGGAACTACCAAAGGAAATTGAAAAACTCACCAATTTGACATTCTTGGGTGTTGGGACACTCAACCTTTCTGGTTCTCTGCCTCCTGAGATGGGGTATTTAAAAACATTAAAAGAGATTTACATTGATAATTCTGGACTTAGCGGTGAGATTCCACCAACATTTGCTAATCTAACAAACTTGGAAATATTTTGGGCAACAGATATAAACCTCACTGGCAGGATACCGGACTTCGTAGGAGATTGGACAAAGCTTACTAAATTGAGGTTACAGGGAACCTCATTTGAAGGTTCCATACCATCTTCACTGTCCAGATTGACTCTTTTGACTGAGCTGCGAATTACTGATTTAACGATTGCCAATGACAACTCTTCTCTTGGATTTATCAAGAATATGACGTCTCTAGAAGTTTTAATGCTGAGGAATAACAATATTTCTGATTCAATTCCTTCCAACATTGGAGATTACCGAAAATTGTTTCAACTGGATTTGAGCTTCAACAATTTGAATGGACAAATACCAGACTCACTATTTCATATGAGTGAGCTTGCCGTCTTGTTTCTTGGAAACAATAAGTTGAATGGTAGCCTTCCTGAGTCTAAAAGCTCGTCTCTTCTCGTTGTAGATTTGTCATACAATAATCTAGTAGGCCAGAGCTTTCCGAGTTGGGTCAACGAACAAAGCCTAGACCTTAACTTGGTGGCCAACAACTTCAGCATAGACAGTTCAAACAGCAGTGGTCTACCTTCAGGGTTGCATTGCCTTCAAAGGGGCTTCCCTTGCCACCGTGATAGTCCAGTTTACTATAACTTCATGATTAACTGCGGTGGTCCTCAGGCTACGATATCCAATGGGACTGTGTATGAGAAGGACATTGAGCCCCTTGGTCCAGCTACATATTATGTGACTGACACAAACAGATGGGGAGTTAGCAATGTCGGTCTTAAAACACAGTTTGTAGCTGCATCATATTTGACTGGGACCAACATTCCAAAGTATACAATATCCACTTCATCTCCAATCGGAAATACTTCAGACCCTACGCTATTTCAGAGTGCAAGGATCTCTGCTTCATCACTCAGATACTATGGCTTGGGTCTTGAGAATGGAAACTATACCGTGAAGCTTGAATTTGCAGAACAAGCTATCCTAGATACCCTTAAAAGAAAAAGTCTTGGAAGACGTGTGTTTGATATATATATCCAGGATGTTCTTGTTGTTAAAGATTTTGATATACGGAAGGAGACAAATAAGACATCTTTGCTAGCTATTGAAAAGGTATACAAGGCTCAGGTGGTTTCAGAGAATTACCTTGAAATCCATTTCTTCTGGGCTGGAAAGGGGACTTGCTGTATACCAGAGGAGGGTACTTATGGACCTCTTGTTTCAGCCATCAGTGCTACACCTGAATTCAAACCTACCGTCAGCAACAAGCTGCCAAGTAGGAAGAAGAATAGGACTGGGCTTATTGTGGGAATTGTTGTTGGTTGTGGAGTTTTAATTCTGGTGGTGCTGCTTTTATATATTGTTCAAGGAAGAAAGAGGCACAACACTGATGATGACTATGATGAAGAGCTGTTGGGAATAGATCTTGGACCTCTCACTTTCAGTTTTTCTGAACTGAAGGCAGCTACAAATGACTTTAGTCCAGATAATAAGCTTGGAGAGGGAGGATTTGGGCCTGTCTTCAAAGGTGCTCTAAATGATGGAAGAGTAATTGCCGTAAAACAATTGTCTGCATCATCCCACCAAGGAAAGAACCAGTTTGTGACTGAGATTGCCACTATATCTTCTGTGCGACACAATAACCTAGTAAATTTGTATGGATTCTGCACCGAGGGAGCCAAACGGCTCCTTGTCTATGAGTATCTGGAGAACAAGAGTCTTGATCAAGCATTATTTGGAAAAAGAGCCTTGCATCTTGATTGGCCGACACGTTTTGATATATGCTTGGGCGTAGCTAGAGGTTTAACTTATCTTCATGAAGAATCAAGGCTTCGAATTGTACACAGAGATGTGAAGGCAAGTAACATCCTGCTTGACTCTAATCTCATTGCCAAAATATCAGATTTTGGTTTGGCGAAGCATTATGATGATAAAAAGACCCACATGAGTACTCGGGTTGCAGGAACATTTGGGTATCTTGCACCGGAATATGCTATGCGTGGACACTTGACAGAGAAAACCGATGTGTTTGCCTATGGTGTTGTCATTCTAGAAATTGTCAGCGGTAGGCCAAATTCTGATCCAAGTTTGGAAGGCGATATGGTTTATCTTCTTGAATTGGCTTGGAACTTGCATGAAAACGAACGTGAAGTTGATCTAGTAGACTCTAGATTATTCGAATTCAATGAGGAAGAAGCAAGACGAATTATCAACATAGGACTTTTGTGCACTCAATCATCACCAATGCTACGACCACCTATGTCTCGCGTGATTGGAATGCTTGCAGGAGATATTGAAGTGACTCCTGCTATTTCAAAGCCCAGTTACTTGACAGACTGGAGATTTGATGATCTAACCAGCCAAACGAAATATGGATGTACTGGCACCAGTCTAGGAACTGATACGTCAACTAGAGGAACTGACTTCAGCTTTTACCACTCATCGGCAAGTACAAGCGTGATGGGGGATGCAGGGCAATTACCTTCAAATGCTACTCTTCCCATACTCAATAATACTAATGGTGATGGTAGGTGA

Protein Analysis

1058

Amino Acids

116.94

Weight (kDa)

5.33

Isoelectric Point (pI)

37.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 140 - 251 3.1e-12 Leucine-rich repeat region
LRR_14 PF23598 261 - 351 8.5e-06 Leucine-rich repeat region
Malectin PF11721 430 - 624 2.5e-39 Malectin domain
PK_Tyr_Ser-Thr PF07714 710 - 972 1.3e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 710 - 906 1.6e-44 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 267
AatII GACGTC 1 cut(s) 878
AccB1I GGYRCC 1 cut(s) 3026
AccI GTMKAC 5 cut(s) 1219, 1475, 1758, 1831, 2805
AccII CGCG 1 cut(s) 2910
AciI CCGC 5 cut(s) 191, 213, 621, 1302, 2706
AclWI GGATC 3 cut(s) 167, 1547, 2717
AcoI YGGCCR 2 cut(s) 1179, 2405
AcsI RAATTY 5 cut(s) 1607, 1886, 2293, 2716, 2822
AcuI CTGAAG 6 cut(s) 117, 1174, 1209, 1494, 2120, 3052
AcyI GRCGYC 1 cut(s) 875
AfaI GTAC 5 cut(s) 1846, 2484, 2593, 3022, 3094
AfeI AGCGCT 1 cut(s) 108
AfiI CCNNNNNNNGG 5 cut(s) 569, 1174, 1246, 1841, 3085
AflIII ACRYGT 2 cut(s) 1660, 2411
AjiI CACGTC 1 cut(s) 1661
AjnI CCWGG 1 cut(s) 1680
AjuI GAANNNNNNNTTGG 2 cut(s) 503, 535
AloI GAACNNNNNNTCC 2 cut(s) 1608, 1640
Alw21I GWGCWC 3 cut(s) 345, 2176, 2873
Alw26I GTCTC 5 cut(s) 203, 882, 1092, 1715, 2910
Alw44I GTGCAC 1 cut(s) 2869
AlwI GGATC 3 cut(s) 167, 1547, 2717
Ama87I CYCGRG 1 cut(s) 2595
Aor51HI AGCGCT 1 cut(s) 108
AoxI GGCC 5 cut(s) 1126, 1179, 2156, 2405, 2711
ApaLI GTGCAC 1 cut(s) 2869
ApeKI GCWGC 9 cut(s) 29, 32, 35, 42, 811, 1439, 1912, 1990, 2105
ApoI RAATTY 5 cut(s) 1607, 1886, 2293, 2716, 2822
ArsI GACNNNNNNTTYG 4 cut(s) 1884, 1916, 2969, 3001
Asp700I GAANNNNTTC 1 cut(s) 1507
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 7 cut(s) 407, 1307, 1364, 1458, 1853, 2072, 2156
AsuHPI GGTGA 5 cut(s) 182, 499, 635, 2874, 3176
AsuII TTCGAA 2 cut(s) 2476, 2820
AsuNHI GCTAGC 1 cut(s) 1741
AvaI CYCGRG 1 cut(s) 2595
AvaII GGWCC 6 cut(s) 407, 1307, 1364, 1458, 1853, 2072
AxyI CCTNAGG 1 cut(s) 1311
BaeGI GKGCMC 1 cut(s) 2873
BalI TGGCCA 1 cut(s) 1181
BanI GGYRCC 1 cut(s) 3026
BanII GRGCYC 2 cut(s) 345, 1359
BarI GAAGNNNNNNTAC 2 cut(s) 756, 788
BbsI GAAGAC 2 cut(s) 1663, 2155
Bbv12I GWGCWC 3 cut(s) 345, 2176, 2873
BbvCI CCTCAGC 1 cut(s) 896
BbvI GCAGC 9 cut(s) 16, 44, 47, 54, 798, 1426, 1899, 1977, 2117
BccI CCATC 7 cut(s) 562, 784, 1392, 1877, 2177, 3097, 3161
BceAI ACGGC 3 cut(s) 1013, 2183, 2342
BcgI CGANNNNNNTGC 2 cut(s) 2654, 2688
BciT130I CCWGG 1 cut(s) 1682
BciVI GTATCC 1 cut(s) 693
BclI TGATCA 1 cut(s) 2365
BcoDI GTCTC 5 cut(s) 203, 882, 1092, 1715, 2910
BfmI CTRYAG 2 cut(s) 30, 40
BfoI RGCGCY 1 cut(s) 110
BfuI GTATCC 1 cut(s) 693
BglII AGATCT 1 cut(s) 2065
BisI GCNGC 9 cut(s) 30, 33, 36, 43, 812, 1440, 1913, 1991, 2106
BlsI GCNGC 9 cut(s) 31, 34, 37, 44, 813, 1441, 1914, 1992, 2107
BmcAI AGTACT 1 cut(s) 2593
Bme1390I CCNGG 1 cut(s) 1682
Bme18I GGWCC 6 cut(s) 407, 1307, 1364, 1458, 1853, 2072
BmeT110I CYCGRG 1 cut(s) 2595
BmgBI CACGTC 1 cut(s) 1661
BmgT120I GGNCC 7 cut(s) 407, 1307, 1364, 1458, 1853, 2072, 2156
BmiI GGNNCC 6 cut(s) 754, 769, 1459, 2320, 2330, 3028
BmrFI CCNGG 1 cut(s) 1682
BmrI ACTGGG 3 cut(s) 1464, 1947, 2958
BmsI GCATC 6 cut(s) 428, 450, 1451, 2225, 2404, 3100
BmtI GCTAGC 1 cut(s) 1745
BmuI ACTGGG 3 cut(s) 1464, 1947, 2958
BoxI GACNNNNGTC 1 cut(s) 2121
BpiI GAAGAC 2 cut(s) 1663, 2155
BpmI CTGGAG 2 cut(s) 2369, 3001
Bpu10I CCTNAGC 2 cut(s) 896, 1767
Bpu14I TTCGAA 2 cut(s) 2476, 2820
BpuEI CTTGAG 1 cut(s) 1598
Bsa29I ATCGAT 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 237
BsaHI GRCGYC 1 cut(s) 875
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 3 cut(s) 1360, 2227, 2313
BsaWI WCCGGW 2 cut(s) 703, 2626
BsaXI ACNNNNNCTCC 2 cut(s) 1477, 1507
Bsc4I CCNNNNNNNGG 5 cut(s) 569, 1174, 1246, 1841, 3085
Bse21I CCTNAGG 1 cut(s) 1311
Bse3DI GCAATG 4 cut(s) 161, 1234, 1417, 2529
Bse8I GATNNNNATC 1 cut(s) 237
BseBI CCWGG 1 cut(s) 1682
BseCI ATCGAT 1 cut(s) 422
BseDI CCNNGG 3 cut(s) 1360, 2227, 2313
BseGI GGATG 5 cut(s) 1690, 2219, 2508, 3023, 3115
BseJI GATNNNNATC 1 cut(s) 237
BseLI CCNNNNNNNGG 5 cut(s) 569, 1174, 1246, 1841, 3085
BseMI GCAATG 4 cut(s) 161, 1234, 1417, 2529
BseMII CTCAG 8 cut(s) 555, 798, 887, 1062, 1325, 1570, 1781, 2241
BseRI GAGGAG 2 cut(s) 233, 1853
BseSI GKGCMC 1 cut(s) 2873
BseXI GCAGC 9 cut(s) 16, 44, 47, 54, 798, 1426, 1899, 1977, 2117
BsgI GTGCAG 1 cut(s) 2293
Bsh1236I CGCG 1 cut(s) 2910
BshFI GGCC 5 cut(s) 1128, 1181, 2158, 2407, 2713
BshNI GGYRCC 1 cut(s) 3026
BshVI ATCGAT 1 cut(s) 422
BsiHKAI GWGCWC 3 cut(s) 345, 2176, 2873
BsiHKCI CYCGRG 1 cut(s) 2595
BsiSI CCGG 2 cut(s) 704, 2627
BslFI GGGAC 5 cut(s) 474, 547, 1345, 1471, 1834
BslI CCNNNNNNNGG 5 cut(s) 569, 1174, 1246, 1841, 3085
BsmAI GTCTC 5 cut(s) 203, 882, 1092, 1715, 2910
BsmBI CGTCTC 2 cut(s) 882, 1092
BsmFI GGGAC 5 cut(s) 474, 547, 1345, 1471, 1834
BsmI GAATGC 2 cut(s) 118, 2925
BsnI GGCC 5 cut(s) 1128, 1181, 2158, 2407, 2713
Bso31I GGTCTC 1 cut(s) 203
BsoBI CYCGRG 1 cut(s) 2595
Bsp119I TTCGAA 2 cut(s) 2476, 2820
Bsp1286I GDGCHC 4 cut(s) 345, 1359, 2176, 2873
Bsp1407I TGTACA 1 cut(s) 2482
Bsp143I GATC 7 cut(s) 172, 1539, 2065, 2365, 2722, 2797, 2992
BspACI CCGC 5 cut(s) 191, 213, 621, 1302, 2706
BspANI GGCC 5 cut(s) 1128, 1181, 2158, 2407, 2713
BspCNI CTCAG 8 cut(s) 556, 799, 888, 1063, 1324, 1569, 1780, 2242
BspDI ATCGAT 1 cut(s) 422
BspFNI CGCG 1 cut(s) 2910
BspHI TCATGA 2 cut(s) 1290, 2458
BspLI GGNNCC 6 cut(s) 754, 769, 1459, 2320, 2330, 3028
BspMAI CTGCAG 2 cut(s) 34, 44
BspOI GCTAGC 1 cut(s) 1745
BspPI GGATC 3 cut(s) 167, 1547, 2717
BspQI GCTCTTC 2 cut(s) 333, 2043
BspT104I TTCGAA 2 cut(s) 2476, 2820
BspT107I GGYRCC 1 cut(s) 3026
BspTNI GGTCTC 1 cut(s) 203
BsrDI GCAATG 4 cut(s) 161, 1234, 1417, 2529
BsrGI TGTACA 1 cut(s) 2482
BssECI CCNNGG 3 cut(s) 1360, 2227, 2313
BssMI GATC 7 cut(s) 172, 1539, 2065, 2365, 2722, 2797, 2992
BssNAI GTATAC 3 cut(s) 1476, 1759, 1832
BssNI GRCGYC 1 cut(s) 875
BssT1I CCWWGG 2 cut(s) 1360, 2227
Bst1107I GTATAC 3 cut(s) 1476, 1759, 1832
Bst2UI CCWGG 1 cut(s) 1682
Bst4CI ACNGT 8 cut(s) 142, 786, 1202, 1265, 1336, 1431, 1597, 1900
Bst6I CTCTTC 6 cut(s) 333, 853, 1095, 2043, 2182, 3144
BstACI GRCGYC 1 cut(s) 875
BstAUI TGTACA 1 cut(s) 2482
BstBI TTCGAA 2 cut(s) 2476, 2820
BstC8I GCNNGC 4 cut(s) 40, 1026, 1743, 2925
BstDEI CTNAG 9 cut(s) 564, 617, 807, 896, 1071, 1311, 1556, 1767, 2250
BstF5I GGATG 5 cut(s) 1690, 2219, 2508, 3023, 3115
BstFNI CGCG 1 cut(s) 2910
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 7 cut(s) 175, 1542, 2068, 2368, 2725, 2800, 2995
BstMAI GTCTC 5 cut(s) 203, 882, 1092, 1715, 2910
BstMBI GATC 7 cut(s) 172, 1539, 2065, 2365, 2722, 2797, 2992
BstMWI GCNNNNNNNGC 5 cut(s) 35, 113, 1257, 1619, 1874
BstNI CCWGG 1 cut(s) 1682
BstPAI GACNNNNGTC 1 cut(s) 2121
BstSCI CCNGG 1 cut(s) 1680
BstSFI CTRYAG 2 cut(s) 30, 40
BstSLI GKGCMC 1 cut(s) 2873
BstUI CGCG 1 cut(s) 2910
BstV1I GCAGC 9 cut(s) 16, 44, 47, 54, 798, 1426, 1899, 1977, 2117
BstV2I GAAGAC 2 cut(s) 1663, 2155
BstX2I RGATCY 2 cut(s) 1539, 2065
BstYI RGATCY 2 cut(s) 1539, 2065
BstZ17I GTATAC 3 cut(s) 1476, 1759, 1832
Bsu15I ATCGAT 1 cut(s) 422
Bsu36I CCTNAGG 1 cut(s) 1311
BsuI GTATCC 1 cut(s) 693
BsuRI GGCC 5 cut(s) 1128, 1181, 2158, 2407, 2713
BsuTUI ATCGAT 1 cut(s) 422
BtrI CACGTC 1 cut(s) 1661
BtsCI GGATG 5 cut(s) 1690, 2219, 2508, 3023, 3115
BtsI GCAGTG 2 cut(s) 187, 1219
BtsIMutI CAGTG 7 cut(s) 5, 187, 690, 782, 1219, 1879, 2028
Cac8I GCNNGC 4 cut(s) 40, 1026, 1743, 2925
CciI TCATGA 2 cut(s) 1290, 2458
CfoI GCGC 1 cut(s) 109
Cfr13I GGNCC 7 cut(s) 407, 1307, 1364, 1458, 1853, 2072, 2156
ClaI ATCGAT 1 cut(s) 422
Csp6I GTAC 5 cut(s) 1845, 2483, 2592, 3021, 3093
CviAII CATG 6 cut(s) 177, 224, 1291, 2459, 2587, 2777
CviQI GTAC 5 cut(s) 1845, 2483, 2592, 3021, 3093
DdeI CTNAG 9 cut(s) 564, 617, 807, 896, 1071, 1311, 1556, 1767, 2250
DpnI GATC 7 cut(s) 174, 1541, 2067, 2367, 2724, 2799, 2994
DpnII GATC 7 cut(s) 172, 1539, 2065, 2365, 2722, 2797, 2992
DraI TTTAAA 1 cut(s) 579
EaeI YGGCCR 2 cut(s) 1179, 2405
Eam1104I CTCTTC 6 cut(s) 333, 853, 1095, 2043, 2182, 3144
EarI CTCTTC 6 cut(s) 333, 853, 1095, 2043, 2182, 3144
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 2 cut(s) 1360, 2227
Eco24I GRGCYC 2 cut(s) 345, 1359
Eco31I GGTCTC 1 cut(s) 203
Eco32I GATATC 1 cut(s) 1323
Eco47I GGWCC 6 cut(s) 407, 1307, 1364, 1458, 1853, 2072
Eco47III AGCGCT 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 343
Eco57I CTGAAG 6 cut(s) 117, 1174, 1209, 1494, 2120, 3052
Eco81I CCTNAGG 1 cut(s) 1311
Eco88I CYCGRG 1 cut(s) 2595
EcoICRI GAGCTC 1 cut(s) 343
EcoO109I RGGNCCY 1 cut(s) 407
EcoRI GAATTC 2 cut(s) 1886, 2822
EcoRII CCWGG 1 cut(s) 1680
EcoRV GATATC 1 cut(s) 1323
EcoT14I CCWWGG 2 cut(s) 1360, 2227
EcoT38I GRGCYC 2 cut(s) 345, 1359
ErhI CCWWGG 2 cut(s) 1360, 2227
Esp3I CGTCTC 2 cut(s) 882, 1092
FaeI CATG 6 cut(s) 180, 227, 1294, 2462, 2590, 2780
FaqI GGGAC 5 cut(s) 474, 547, 1345, 1471, 1834
FatI CATG 6 cut(s) 176, 223, 1290, 2458, 2586, 2776
FauNDI CATATG 1 cut(s) 1014
FbaI TGATCA 1 cut(s) 2365
FblI GTMKAC 5 cut(s) 1219, 1475, 1758, 1831, 2805
Fnu4HI GCNGC 9 cut(s) 30, 33, 36, 43, 812, 1440, 1913, 1991, 2106
FokI GGATG 5 cut(s) 1697, 2206, 2495, 3030, 3122
FriOI GRGCYC 2 cut(s) 345, 1359
Fsp4HI GCNGC 9 cut(s) 30, 33, 36, 43, 812, 1440, 1913, 1991, 2106
GlaI GCGC 1 cut(s) 108
GluI GCNGC 9 cut(s) 30, 33, 36, 43, 812, 1440, 1913, 1991, 2106
GsuI CTGGAG 2 cut(s) 2369, 3001
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 5 cut(s) 1128, 1181, 2158, 2407, 2713
HapII CCGG 2 cut(s) 704, 2627
HhaI GCGC 1 cut(s) 109
Hin1I GRCGYC 1 cut(s) 875
Hin1II CATG 6 cut(s) 180, 227, 1294, 2462, 2590, 2780
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HincII GTYRAC 2 cut(s) 1150, 3051
HindII GTYRAC 2 cut(s) 1150, 3051
HindIII AAGCTT 3 cut(s) 728, 1601, 2135
HpaII CCGG 2 cut(s) 704, 2627
HphI GGTGA 5 cut(s) 182, 499, 635, 2874, 3176
HpyCH4III ACNGT 8 cut(s) 142, 786, 1202, 1265, 1336, 1431, 1597, 1900
HpyCH4IV ACGT 5 cut(s) 875, 1660, 2413, 2788, 3047
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 113, 1257, 1619, 1874
HpyF3I CTNAG 9 cut(s) 564, 617, 807, 896, 1071, 1311, 1556, 1767, 2250
HpySE526I ACGT 5 cut(s) 875, 1660, 2413, 2788, 3047
Hsp92I GRCGYC 1 cut(s) 875
Hsp92II CATG 6 cut(s) 180, 227, 1294, 2462, 2590, 2780
HspAI GCGC 1 cut(s) 107
Ksp22I TGATCA 1 cut(s) 2365
Kzo9I GATC 7 cut(s) 172, 1539, 2065, 2365, 2722, 2797, 2992
LguI GCTCTTC 2 cut(s) 333, 2043
LmnI GCTCC 3 cut(s) 331, 2318, 2334
Lsp1109I GCAGC 9 cut(s) 16, 44, 47, 54, 798, 1426, 1899, 1977, 2117
LweI GCATC 6 cut(s) 428, 450, 1451, 2225, 2404, 3100
MaeII ACGT 5 cut(s) 875, 1660, 2413, 2788, 3047
MaeIII GTNAC 7 cut(s) 3, 744, 1381, 2245, 2504, 2941, 2966
MalI GATC 7 cut(s) 174, 1541, 2067, 2367, 2724, 2799, 2994
MboI GATC 7 cut(s) 172, 1539, 2065, 2365, 2722, 2797, 2992
MfeI CAATTG 1 cut(s) 2207
MflI RGATCY 2 cut(s) 1539, 2065
MhlI GDGCHC 4 cut(s) 345, 1359, 2176, 2873
MlsI TGGCCA 1 cut(s) 1181
MluNI TGGCCA 1 cut(s) 1181
MlyI GAGTC 7 cut(s) 790, 995, 1082, 2368, 2513, 2801, 2938
MmeI TCCRAC 1 cut(s) 954
Mox20I TGGCCA 1 cut(s) 1181
MroXI GAANNNNTTC 1 cut(s) 1507
MscI TGGCCA 1 cut(s) 1181
MslI CAYNNNNRTG 4 cut(s) 228, 1017, 2490, 2568
Msp20I TGGCCA 1 cut(s) 1181
MspA1I CMGCKG 1 cut(s) 2706
MspI CCGG 2 cut(s) 704, 2627
MspR9I CCNGG 1 cut(s) 1682
MunI CAATTG 1 cut(s) 2207
Mva1269I GAATGC 2 cut(s) 118, 2925
MvaI CCWGG 1 cut(s) 1682
MvnI CGCG 1 cut(s) 2910
MwoI GCNNNNNNNGC 5 cut(s) 35, 113, 1257, 1619, 1874
NdeI CATATG 1 cut(s) 1014
NdeII GATC 7 cut(s) 172, 1539, 2065, 2365, 2722, 2797, 2992
NheI GCTAGC 1 cut(s) 1741
NlaIII CATG 6 cut(s) 180, 227, 1294, 2462, 2590, 2780
NlaIV GGNNCC 6 cut(s) 754, 769, 1459, 2320, 2330, 3028
NmuCI GTSAC 4 cut(s) 3, 1381, 2245, 2941
NspV TTCGAA 2 cut(s) 2476, 2820
PagI TCATGA 2 cut(s) 1290, 2458
PciSI GCTCTTC 2 cut(s) 333, 2043
PctI GAATGC 2 cut(s) 118, 2925
PdmI GAANNNNTTC 1 cut(s) 1507
PfeI GAWTC 4 cut(s) 628, 917, 2304, 2465
PfoI TCCNGGA 1 cut(s) 1680
PkrI GCNGC 9 cut(s) 31, 34, 37, 44, 813, 1441, 1914, 1992, 2107
PleI GAGTC 7 cut(s) 790, 995, 1081, 2367, 2513, 2801, 2938
PpsI GAGTC 7 cut(s) 790, 995, 1081, 2367, 2513, 2801, 2938
PpuMI RGGWCCY 1 cut(s) 407
PshAI GACNNNNGTC 1 cut(s) 2121
PsiI TTATAA 1 cut(s) 267
Psp124BI GAGCTC 1 cut(s) 345
Psp5II RGGWCCY 1 cut(s) 407
Psp6I CCWGG 1 cut(s) 1680
PspGI CCWGG 1 cut(s) 1680
PspN4I GGNNCC 6 cut(s) 754, 769, 1459, 2320, 2330, 3028
PspPI GGNCC 7 cut(s) 407, 1307, 1364, 1458, 1853, 2072, 2156
PspPPI RGGWCCY 1 cut(s) 407
PstI CTGCAG 2 cut(s) 34, 44
PsuI RGATCY 2 cut(s) 1539, 2065
RsaI GTAC 5 cut(s) 1846, 2484, 2593, 3022, 3094
RsaNI GTAC 5 cut(s) 1845, 2483, 2592, 3021, 3093
RseI CAYNNNNRTG 4 cut(s) 228, 1017, 2490, 2568
SacI GAGCTC 1 cut(s) 345
SapI GCTCTTC 2 cut(s) 333, 2043
SatI GCNGC 9 cut(s) 30, 33, 36, 43, 812, 1440, 1913, 1991, 2106
Sau3AI GATC 7 cut(s) 172, 1539, 2065, 2365, 2722, 2797, 2992
Sau96I GGNCC 7 cut(s) 407, 1307, 1364, 1458, 1853, 2072, 2156
ScaI AGTACT 1 cut(s) 2593
SchI GAGTC 7 cut(s) 790, 995, 1082, 2368, 2513, 2801, 2938
ScrFI CCNGG 1 cut(s) 1682
SduI GDGCHC 4 cut(s) 345, 1359, 2176, 2873
SfaNI GCATC 6 cut(s) 428, 450, 1451, 2225, 2404, 3100
SfcI CTRYAG 2 cut(s) 30, 40
SfuI TTCGAA 2 cut(s) 2476, 2820
SinI GGWCC 6 cut(s) 407, 1307, 1364, 1458, 1853, 2072
SmiMI CAYNNNNRTG 4 cut(s) 228, 1017, 2490, 2568
SmlI CTYRAG 1 cut(s) 1577
SmoI CTYRAG 1 cut(s) 1577
SsiI CCGC 5 cut(s) 191, 213, 621, 1302, 2706
SspI AATATT 3 cut(s) 136, 666, 910
SstI GAGCTC 1 cut(s) 345
StyD4I CCNGG 1 cut(s) 1680
StyI CCWWGG 2 cut(s) 1360, 2227
TaaI ACNGT 8 cut(s) 142, 786, 1202, 1265, 1336, 1431, 1597, 1900
TaiI ACGT 5 cut(s) 878, 1663, 2416, 2791, 3050
TaqI TCGA 3 cut(s) 422, 2476, 2820
TaqII GACCGA 1 cut(s) 1406
TatI WGTACW 4 cut(s) 2482, 2591, 3020, 3092
TfiI GAWTC 4 cut(s) 628, 917, 2304, 2465
TscAI CASTG 7 cut(s) 12, 187, 697, 789, 1219, 1879, 2035
TseFI GTSAC 4 cut(s) 3, 1381, 2245, 2941
TseI GCWGC 9 cut(s) 29, 32, 35, 42, 811, 1439, 1912, 1990, 2105
Tsp45I GTSAC 4 cut(s) 3, 1381, 2245, 2941
TspGWI ACGGA 1 cut(s) 1729
TspRI CASTG 7 cut(s) 12, 187, 697, 789, 1219, 1879, 2035
VneI GTGCAC 1 cut(s) 2869
VpaK11BI GGWCC 6 cut(s) 407, 1307, 1364, 1458, 1853, 2072
XapI RAATTY 5 cut(s) 1607, 1886, 2293, 2716, 2822
XbaI TCTAGA 3 cut(s) 880, 2692, 2810
XcmI CCANNNNNNNNNTGG 1 cut(s) 3011
XmiI GTMKAC 5 cut(s) 1219, 1475, 1758, 1831, 2805
XmnI GAANNNNTTC 1 cut(s) 1507
ZraI GACGTC 1 cut(s) 876
ZrmI AGTACT 1 cut(s) 2593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.