MD15G1239400.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000088
Physical Location & Seq
Forward (+)
19702631 .. 19706174
3544 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1239400.v1.1.491

Sequence Viewer

Length: 1941 bp
ATGCACGGTGCATGTATGTGCTTGTGTATACTCCGACTTGTCTTACAATTTTTATCAGGAAGCTTTCCCCAGTGGGTGACCACAATGTGGGTGGCCCCAATACCGCAACTGAACGTAGTGGTCAACAACTTCACATTTAACAGTTCAAACATAACTCTTCCTGGATTGAATTGCCTCCAGAGAAATTTTCCATGCAATCGAAATACCCCACGATCAAGCTTCTCAATCAACTGTGGTGGACAGCAAATGACAGGAAGTGATGGCATATTGTATGAGAGTGACGACTCAGAACTTGGTCTAACATCATTCCGTGTAACCAGTACAGAGAATGGGGCTGTCAGCAATGCCGGTTCGATTTCTTACGATACTTCGTTTTCTGACGATACCCCATCGTTGAACAAGACCCTTGCACAAGTCACCGGAACAGATGTGACCCCGGTGCTTTTCCAGACTTCAAGAATGTCCACAGGATCACTGAGATACTATGGCCTGGGCCTTGAGAATGGGCTCTACACTGTAACATTGCACTTTGCAGAGACGGCTTATGAAAGTCGCACTTCGCAAACTCGGCAAAGTCTAGGACGACGTGTATTTGATATCTATATTCAGGGTACCCGAAGGACGAAGGACTTTGACATATCGAAGGAGGCAAGTGGTGTTAACCGAGCAGTTGTGAGAAAATTTAATGTTAACATGTCAGAGAATTATCTTGAAATTCATCTATTCTGGGCTGGTGAAGGGACTTGTTGCATACCCGATTCTGGTGATTTTGGCCCACTAATAGCAGCTGTCCATGCTGCTTCAGATTTTACAAAGAAGAGCAATACTGGGTTGATAGTTGGTATTGCAGTTCTTGTTGGAGTTGTGAGCTTGCTATTAATATTTGCAATTCTATATATGAGGAGGAAAAAATCAGAAAAAGAAGACGATGAAGATATTCTAGGATTAGGCCCCCGGCCATATACTTTCAGTTATGTTGAATTGAGAAATGCAACTAAAGATTTTAATCCTTCAAATAAGCTAGGAGAGGGAGGATATGGCCCCGTTTATAAGGGTACACTTTCTGATGGTAGAGTAGTGGCTGTGAAGCAACTTTCAGTATCATTTCACCAAGGGAAGAGTCAATTTGTATCTGAAATTGCTACCATATCTGCTGTGCAACATCGGAATCTAGTGAAATTGTATGGATGCTGCATTGAAGGCAGACACCGCATTTTGGTTTATGAGTATCTTGAAAACAAGAGCCTTGATCAGGCACTTTTTGTAACTTACTGGCCTACTCGATTCAATATATTGTTGGGAACAGCAAGAGGATTTGCTTACCTTCATGAGGAGTCAAGGCCAAGGATTGTACATCGAGATGTCAAAGCAAATTTTGGACTGGCAAAGCTTTATGATGACGAAAAAACCCACATCAGCACCCGGGTTGCAGGGACAATAGGCTATTTGGCACCGGAGTATGCAATGTTTGGACATTTGACAGAGAAGGCCGATGTGTTTGGTTTTGGAGTCGTGTGGACTCTACATGAAAACGACCAAACTCTAGGGCTGGAGGATCCAAGATTGACAGAGTTTGATGAAACTGAAGCAACTAGATTGATAAGAGTAGCTCTCATGTGCACGCAGGGATCACCGATGGCACGGCCATCTATGTCACGCGTGGTTGCAATGCTCTCTGGAGACATTGACATAGGCAATGTCATGTCGAAGCCAAACTATTTGACAGATTATGATTTTAAAGATGTAACAACATCGTTAACAAGTAGATTTTTGGTGGAGGATGATACCCCATCAACTTCATCCAAGGGTAGTAATGTTCGCCTCAACTATCAGTCGAGAGGCAGCAATGCAAGTGGTGTTAACACTCCCTGGATTGACCATGCGCCTTCTATAAACGTCACTCAATCACTGCTCGTTGAAATTAGAGGAGAAGGAAGGTGA

Protein Analysis

647

Amino Acids

71.48

Weight (kDa)

6.38

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 74 - 264 1.8e-36 Malectin domain
Pkinase PF00069 337 - 506 1.9e-25 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 338 - 506 6.6e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1050
Acc65I GGTACC 1 cut(s) 611
AccB1I GGYRCC 2 cut(s) 611, 1450
AccB7I CCANNNNNTGG 1 cut(s) 87
AccI GTMKAC 1 cut(s) 28
AccII CGCG 1 cut(s) 1659
AciI CCGC 2 cut(s) 104, 1210
AclWI GGATC 4 cut(s) 478, 1550, 1563, 1636
AcoI YGGCCR 2 cut(s) 956, 1643
AcsI RAATTY 4 cut(s) 184, 680, 714, 1372
AcuI CTGAAG 2 cut(s) 786, 1605
AdeI CACNNNGTG 1 cut(s) 87
AfaI GTAC 4 cut(s) 322, 613, 1057, 1353
AfiI CCNNNNNNNGG 5 cut(s) 87, 761, 1216, 1252, 1330
AflIII ACRYGT 3 cut(s) 586, 693, 1657
AjiI CACGTC 1 cut(s) 587
AjnI CCWGG 3 cut(s) 160, 489, 1868
AluBI AGCT 7 cut(s) 63, 219, 788, 870, 1021, 1390, 1610
AluI AGCT 7 cut(s) 63, 219, 788, 870, 1021, 1390, 1610
Alw21I GWGCWC 1 cut(s) 1622
Alw26I GTCTC 2 cut(s) 530, 1674
Alw44I GTGCAC 1 cut(s) 1618
AlwI GGATC 4 cut(s) 478, 1550, 1563, 1636
Ama87I CYCGRG 1 cut(s) 1422
ApaLI GTGCAC 1 cut(s) 1618
ApeKI GCWGC 4 cut(s) 785, 797, 1191, 1842
ApoI RAATTY 4 cut(s) 184, 680, 714, 1372
AseI ATTAAT 1 cut(s) 878
Asp700I GAANNNNTTC 1 cut(s) 936
Asp718I GGTACC 1 cut(s) 611
AspLEI GCGC 1 cut(s) 1885
AspS9I GGNCC 5 cut(s) 94, 493, 773, 950, 1040
AsuC2I CCSGG 4 cut(s) 437, 955, 1423, 1424
AsuHPI GGTGA 6 cut(s) 88, 409, 746, 776, 1100, 1623
AvaI CYCGRG 1 cut(s) 1422
BaeGI GKGCMC 1 cut(s) 1622
BamHI GGATCC 1 cut(s) 1555
BanI GGYRCC 2 cut(s) 611, 1450
BanII GRGCYC 1 cut(s) 510
BbsI GAAGAC 1 cut(s) 930
Bbv12I GWGCWC 1 cut(s) 1622
BbvI GCAGC 4 cut(s) 784, 797, 1178, 1854
BccI CCATC 6 cut(s) 254, 397, 1061, 1630, 1654, 1798
BceAI ACGGC 2 cut(s) 555, 1658
BciT130I CCWGG 3 cut(s) 162, 491, 1870
BclI TGATCA 1 cut(s) 1249
BcnI CCSGG 4 cut(s) 437, 955, 1423, 1424
BcoDI GTCTC 2 cut(s) 530, 1674
BfaI CTAG 6 cut(s) 578, 941, 1022, 1172, 1544, 1593
BisI GCNGC 4 cut(s) 786, 798, 1192, 1843
BlsI GCNGC 4 cut(s) 787, 799, 1193, 1844
Bme1390I CCNGG 7 cut(s) 162, 437, 491, 955, 1423, 1424, 1870
BmeT110I CYCGRG 1 cut(s) 1422
BmgBI CACGTC 1 cut(s) 587
BmgT120I GGNCC 5 cut(s) 94, 493, 773, 950, 1040
BmiI GGNNCC 6 cut(s) 96, 613, 952, 1042, 1452, 1557
BmrFI CCNGG 7 cut(s) 162, 437, 491, 955, 1423, 1424, 1870
BmrI ACTGGG 2 cut(s) 64, 837
BmsI GCATC 1 cut(s) 1178
BmuI ACTGGG 2 cut(s) 64, 837
BpiI GAAGAC 1 cut(s) 930
BplI GAGNNNNNCTC 2 cut(s) 1596, 1628
BpmI CTGGAG 3 cut(s) 161, 1571, 1698
BpuEI CTTGAG 1 cut(s) 518
BpuMI CCSGG 4 cut(s) 437, 955, 1423, 1424
BsaBI GATNNNNATC 1 cut(s) 1005
BsaJI CCNNGG 8 cut(s) 435, 490, 953, 1111, 1343, 1422, 1803, 1868
BsaWI WCCGGW 2 cut(s) 419, 1453
Bsc4I CCNNNNNNNGG 5 cut(s) 87, 761, 1216, 1252, 1330
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 5 cut(s) 70, 318, 832, 1277, 1386
Bse3DI GCAATG 6 cut(s) 349, 521, 1470, 1674, 1702, 1852
Bse8I GATNNNNATC 1 cut(s) 1005
BseBI CCWGG 3 cut(s) 162, 491, 1870
BseDI CCNNGG 8 cut(s) 435, 490, 953, 1111, 1343, 1422, 1803, 1868
BseGI GGATG 3 cut(s) 1193, 1786, 1799
BseJI GATNNNNATC 1 cut(s) 1005
BseLI CCNNNNNNNGG 5 cut(s) 87, 761, 1216, 1252, 1330
BseMI GCAATG 6 cut(s) 349, 521, 1470, 1674, 1702, 1852
BseMII CTCAG 2 cut(s) 300, 467
BseNI ACTGG 5 cut(s) 70, 318, 832, 1277, 1386
BseRI GAGGAG 3 cut(s) 916, 1346, 1941
BseSI GKGCMC 1 cut(s) 1622
BseXI GCAGC 4 cut(s) 784, 797, 1178, 1854
Bsh1236I CGCG 1 cut(s) 1659
BshNI GGYRCC 2 cut(s) 611, 1450
BsiHKAI GWGCWC 1 cut(s) 1622
BsiHKCI CYCGRG 1 cut(s) 1422
BsiSI CCGG 6 cut(s) 348, 420, 437, 955, 1423, 1454
BslFI GGGAC 2 cut(s) 754, 1447
BslI CCNNNNNNNGG 5 cut(s) 87, 761, 1216, 1252, 1330
BsmAI GTCTC 2 cut(s) 530, 1674
BsmBI CGTCTC 1 cut(s) 530
BsmFI GGGAC 2 cut(s) 754, 1447
BsoBI CYCGRG 1 cut(s) 1422
Bsp1286I GDGCHC 2 cut(s) 510, 1622
Bsp1407I TGTACA 1 cut(s) 1351
Bsp143I GATC 5 cut(s) 212, 470, 1249, 1555, 1628
BspACI CCGC 2 cut(s) 104, 1210
BspCNI CTCAG 2 cut(s) 299, 468
BspFNI CGCG 1 cut(s) 1659
BspHI TCATGA 1 cut(s) 1327
BspLI GGNNCC 6 cut(s) 96, 613, 952, 1042, 1452, 1557
BspPI GGATC 4 cut(s) 478, 1550, 1563, 1636
BspQI GCTCTTC 1 cut(s) 812
BspT107I GGYRCC 2 cut(s) 611, 1450
BsrDI GCAATG 6 cut(s) 349, 521, 1470, 1674, 1702, 1852
BsrFI RCCGGY 1 cut(s) 347
BsrGI TGTACA 1 cut(s) 1351
BsrI ACTGG 5 cut(s) 70, 318, 832, 1277, 1386
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 8 cut(s) 435, 490, 953, 1111, 1343, 1422, 1803, 1868
BssMI GATC 5 cut(s) 212, 470, 1249, 1555, 1628
BssNAI GTATAC 1 cut(s) 29
BssT1I CCWWGG 3 cut(s) 1111, 1343, 1803
Bst1107I GTATAC 1 cut(s) 29
Bst2UI CCWGG 3 cut(s) 162, 491, 1870
Bst4CI ACNGT 4 cut(s) 8, 143, 233, 517
Bst6I CTCTTC 3 cut(s) 162, 812, 1112
BstAUI TGTACA 1 cut(s) 1351
BstC8I GCNNGC 2 cut(s) 872, 1622
BstDEI CTNAG 2 cut(s) 286, 476
BstEII GGTNACC 1 cut(s) 76
BstENI CCTNNNNNAGG 2 cut(s) 1250, 1328
BstF5I GGATG 3 cut(s) 1193, 1786, 1799
BstFNI CGCG 1 cut(s) 1659
BstHHI GCGC 1 cut(s) 1885
BstKTI GATC 5 cut(s) 215, 473, 1252, 1558, 1631
BstMAI GTCTC 2 cut(s) 530, 1674
BstMBI GATC 5 cut(s) 212, 470, 1249, 1555, 1628
BstMWI GCNNNNNNNGC 5 cut(s) 539, 568, 794, 1200, 1209
BstNI CCWGG 3 cut(s) 162, 491, 1870
BstNSI RCATGY 2 cut(s) 15, 697
BstPI GGTNACC 1 cut(s) 76
BstSCI CCNGG 7 cut(s) 160, 435, 489, 953, 1421, 1422, 1868
BstSLI GKGCMC 1 cut(s) 1622
BstUI CGCG 1 cut(s) 1659
BstV1I GCAGC 4 cut(s) 784, 797, 1178, 1854
BstV2I GAAGAC 1 cut(s) 930
BstX2I RGATCY 1 cut(s) 1555
BstYI RGATCY 1 cut(s) 1555
BstZ17I GTATAC 1 cut(s) 29
BtrI CACGTC 1 cut(s) 587
BtsCI GGATG 3 cut(s) 1193, 1786, 1799
BtsI GCAGTG 1 cut(s) 1907
BtsIMutI CAGTG 4 cut(s) 77, 473, 513, 1907
Cac8I GCNNGC 2 cut(s) 872, 1622
CciI TCATGA 1 cut(s) 1327
CfoI GCGC 1 cut(s) 1885
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 5 cut(s) 94, 493, 773, 950, 1040
Cfr9I CCCGGG 1 cut(s) 1422
Csp6I GTAC 4 cut(s) 321, 612, 1056, 1352
CspCI CAANNNNNGTGG 6 cut(s) 72, 107, 217, 252, 1834, 1869
CviAII CATG 9 cut(s) 12, 192, 694, 794, 1328, 1526, 1615, 1702, 1880
CviQI GTAC 4 cut(s) 321, 612, 1056, 1352
DdeI CTNAG 2 cut(s) 286, 476
DpnI GATC 5 cut(s) 214, 472, 1251, 1557, 1630
DpnII GATC 5 cut(s) 212, 470, 1249, 1555, 1628
DraI TTTAAA 1 cut(s) 1738
DraIII CACNNNGTG 1 cut(s) 87
EaeI YGGCCR 2 cut(s) 956, 1643
Eam1104I CTCTTC 3 cut(s) 162, 812, 1112
EarI CTCTTC 3 cut(s) 162, 812, 1112
Eco130I CCWWGG 3 cut(s) 1111, 1343, 1803
Eco24I GRGCYC 1 cut(s) 510
Eco32I GATATC 1 cut(s) 598
Eco57I CTGAAG 2 cut(s) 786, 1605
Eco88I CYCGRG 1 cut(s) 1422
Eco91I GGTNACC 1 cut(s) 76
EcoNI CCTNNNNNAGG 2 cut(s) 1250, 1328
EcoO109I RGGNCCY 1 cut(s) 950
EcoO65I GGTNACC 1 cut(s) 76
EcoRII CCWGG 3 cut(s) 160, 489, 1868
EcoRV GATATC 1 cut(s) 598
EcoT14I CCWWGG 3 cut(s) 1111, 1343, 1803
EcoT38I GRGCYC 1 cut(s) 510
ErhI CCWWGG 3 cut(s) 1111, 1343, 1803
Esp3I CGTCTC 1 cut(s) 530
FaeI CATG 9 cut(s) 15, 195, 697, 797, 1331, 1529, 1618, 1705, 1883
FalI AAGNNNNNCTT 2 cut(s) 541, 573
FaqI GGGAC 2 cut(s) 754, 1447
FatI CATG 9 cut(s) 11, 191, 693, 793, 1327, 1525, 1614, 1701, 1879
FbaI TGATCA 1 cut(s) 1249
FblI GTMKAC 1 cut(s) 28
Fnu4HI GCNGC 4 cut(s) 786, 798, 1192, 1843
FokI GGATG 3 cut(s) 1200, 1786, 1793
FriOI GRGCYC 1 cut(s) 510
Fsp4HI GCNGC 4 cut(s) 786, 798, 1192, 1843
FspBI CTAG 6 cut(s) 578, 941, 1022, 1172, 1544, 1593
GlaI GCGC 1 cut(s) 1884
GluI GCNGC 4 cut(s) 786, 798, 1192, 1843
GsuI CTGGAG 3 cut(s) 161, 1571, 1698
HapII CCGG 6 cut(s) 348, 420, 437, 955, 1423, 1454
HhaI GCGC 1 cut(s) 1885
Hin1II CATG 9 cut(s) 15, 195, 697, 797, 1331, 1529, 1618, 1705, 1883
Hin6I GCGC 1 cut(s) 1883
HinP1I GCGC 1 cut(s) 1883
HincII GTYRAC 5 cut(s) 124, 661, 691, 1758, 1861
HindII GTYRAC 5 cut(s) 124, 661, 691, 1758, 1861
HindIII AAGCTT 3 cut(s) 61, 217, 1388
HinfI GANTC 8 cut(s) 284, 758, 1120, 1168, 1284, 1334, 1509, 1519
HpaI GTTAAC 4 cut(s) 661, 691, 1758, 1861
HpaII CCGG 6 cut(s) 348, 420, 437, 955, 1423, 1454
HphI GGTGA 6 cut(s) 88, 409, 746, 776, 1100, 1623
Hpy188I TCNGA 9 cut(s) 35, 289, 379, 700, 805, 916, 1066, 1135, 1167
Hpy99I CGWCG 1 cut(s) 588
HpyCH4III ACNGT 4 cut(s) 8, 143, 233, 517
HpyCH4IV ACGT 3 cut(s) 114, 586, 1896
HpyF10VI GCNNNNNNNGC 5 cut(s) 539, 568, 794, 1200, 1209
HpyF3I CTNAG 2 cut(s) 286, 476
HpySE526I ACGT 3 cut(s) 114, 586, 1896
Hsp92II CATG 9 cut(s) 15, 195, 697, 797, 1331, 1529, 1618, 1705, 1883
HspAI GCGC 1 cut(s) 1883
KpnI GGTACC 1 cut(s) 615
Ksp22I TGATCA 1 cut(s) 1249
KspAI GTTAAC 4 cut(s) 661, 691, 1758, 1861
Kzo9I GATC 5 cut(s) 212, 470, 1249, 1555, 1628
LguI GCTCTTC 1 cut(s) 812
Lsp1109I GCAGC 4 cut(s) 784, 797, 1178, 1854
LweI GCATC 1 cut(s) 1178
MaeI CTAG 6 cut(s) 578, 941, 1022, 1172, 1544, 1593
MaeII ACGT 3 cut(s) 114, 586, 1896
MalI GATC 5 cut(s) 214, 472, 1251, 1557, 1630
MboI GATC 5 cut(s) 212, 470, 1249, 1555, 1628
MboII GAAGA 5 cut(s) 149, 829, 935, 944, 1129
MflI RGATCY 1 cut(s) 1555
MhlI GDGCHC 2 cut(s) 510, 1622
MluI ACGCGT 1 cut(s) 1657
MlyI GAGTC 5 cut(s) 278, 1129, 1343, 1513, 1518
MmeI TCCRAC 2 cut(s) 58, 838
MroXI GAANNNNTTC 1 cut(s) 936
MseI TTAA 9 cut(s) 138, 660, 684, 690, 878, 1005, 1737, 1757, 1860
MslI CAYNNNNRTG 2 cut(s) 16, 1359
MspA1I CMGCKG 1 cut(s) 788
MspI CCGG 6 cut(s) 348, 420, 437, 955, 1423, 1454
MspR9I CCNGG 7 cut(s) 162, 437, 491, 955, 1423, 1424, 1870
MvaI CCWGG 3 cut(s) 162, 491, 1870
MvnI CGCG 1 cut(s) 1659
MwoI GCNNNNNNNGC 5 cut(s) 539, 568, 794, 1200, 1209
NciI CCSGG 4 cut(s) 437, 955, 1423, 1424
NdeII GATC 5 cut(s) 212, 470, 1249, 1555, 1628
NlaIII CATG 9 cut(s) 15, 195, 697, 797, 1331, 1529, 1618, 1705, 1883
NlaIV GGNNCC 6 cut(s) 96, 613, 952, 1042, 1452, 1557
NmeAIII GCCGAG 1 cut(s) 547
NmuCI GTSAC 6 cut(s) 76, 278, 415, 430, 1653, 1897
NspI RCATGY 2 cut(s) 15, 697
PagI TCATGA 1 cut(s) 1327
PciI ACATGT 1 cut(s) 693
PciSI GCTCTTC 1 cut(s) 812
PdmI GAANNNNTTC 1 cut(s) 936
PfeI GAWTC 3 cut(s) 758, 1168, 1284
PflMI CCANNNNNTGG 1 cut(s) 87
PfoI TCCNGGA 1 cut(s) 160
PkrI GCNGC 4 cut(s) 787, 799, 1193, 1844
PleI GAGTC 5 cut(s) 278, 1128, 1342, 1513, 1517
PpsI GAGTC 5 cut(s) 278, 1128, 1342, 1513, 1517
PscI ACATGT 1 cut(s) 693
PshBI ATTAAT 1 cut(s) 878
PsiI TTATAA 1 cut(s) 1050
Psp6I CCWGG 3 cut(s) 160, 489, 1868
PspEI GGTNACC 1 cut(s) 76
PspGI CCWGG 3 cut(s) 160, 489, 1868
PspN4I GGNNCC 6 cut(s) 96, 613, 952, 1042, 1452, 1557
PspPI GGNCC 5 cut(s) 94, 493, 773, 950, 1040
PsuI RGATCY 1 cut(s) 1555
PvuII CAGCTG 1 cut(s) 788
RsaI GTAC 4 cut(s) 322, 613, 1057, 1353
RsaNI GTAC 4 cut(s) 321, 612, 1056, 1352
RseI CAYNNNNRTG 2 cut(s) 16, 1359
SapI GCTCTTC 1 cut(s) 812
SaqAI TTAA 9 cut(s) 138, 660, 684, 690, 878, 1005, 1737, 1757, 1860
SatI GCNGC 4 cut(s) 786, 798, 1192, 1843
Sau3AI GATC 5 cut(s) 212, 470, 1249, 1555, 1628
Sau96I GGNCC 5 cut(s) 94, 493, 773, 950, 1040
SchI GAGTC 5 cut(s) 278, 1129, 1343, 1513, 1518
ScrFI CCNGG 7 cut(s) 162, 437, 491, 955, 1423, 1424, 1870
SduI GDGCHC 2 cut(s) 510, 1622
SfaNI GCATC 1 cut(s) 1178
SmaI CCCGGG 1 cut(s) 1424
SmiMI CAYNNNNRTG 2 cut(s) 16, 1359
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
SsiI CCGC 2 cut(s) 104, 1210
SspI AATATT 1 cut(s) 882
SspMI CTAG 6 cut(s) 578, 941, 1022, 1172, 1544, 1593
StyD4I CCNGG 7 cut(s) 160, 435, 489, 953, 1421, 1422, 1868
StyI CCWWGG 3 cut(s) 1111, 1343, 1803
TaaI ACNGT 4 cut(s) 8, 143, 233, 517
TaiI ACGT 3 cut(s) 117, 589, 1899
TaqI TCGA 7 cut(s) 199, 353, 641, 1282, 1357, 1706, 1835
TatI WGTACW 2 cut(s) 320, 1351
TfiI GAWTC 3 cut(s) 758, 1168, 1284
Tru1I TTAA 9 cut(s) 138, 660, 684, 690, 878, 1005, 1737, 1757, 1860
Tru9I TTAA 9 cut(s) 138, 660, 684, 690, 878, 1005, 1737, 1757, 1860
TscAI CASTG 4 cut(s) 77, 480, 520, 1914
TseFI GTSAC 6 cut(s) 76, 278, 415, 430, 1653, 1897
TseI GCWGC 4 cut(s) 785, 797, 1191, 1842
Tsp45I GTSAC 6 cut(s) 76, 278, 415, 430, 1653, 1897
TspDTI ATGAA 7 cut(s) 561, 707, 945, 1316, 1542, 1593, 1788
TspGWI ACGGA 1 cut(s) 299
TspMI CCCGGG 1 cut(s) 1422
TspRI CASTG 4 cut(s) 77, 480, 520, 1914
Van91I CCANNNNNTGG 1 cut(s) 87
VneI GTGCAC 1 cut(s) 1618
VspI ATTAAT 1 cut(s) 878
XagI CCTNNNNNAGG 2 cut(s) 1250, 1328
XapI RAATTY 4 cut(s) 184, 680, 714, 1372
XceI RCATGY 2 cut(s) 15, 697
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XmaI CCCGGG 1 cut(s) 1422
XmiI GTMKAC 1 cut(s) 28
XmnI GAANNNNTTC 1 cut(s) 936
XspI CTAG 6 cut(s) 578, 941, 1022, 1172, 1544, 1593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.