Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 326 |
| AccIII | TCCGGA | 1 cut(s) | 378 |
| AclWI | GGATC | 4 cut(s) | 166, 179, 359, 398 |
| AcsI | RAATTY | 2 cut(s) | 58, 652 |
| AcuI | CTGAAG | 4 cut(s) | 31, 66, 345, 440 |
| AfaI | GTAC | 1 cut(s) | 532 |
| AflIII | ACRYGT | 1 cut(s) | 505 |
| AgsI | TTSAA | 2 cut(s) | 63, 91 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 219 |
| AjiI | CACGTC | 1 cut(s) | 506 |
| AjnI | CCWGG | 2 cut(s) | 127, 525 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 93, 125 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 459, 491 |
| AluBI | AGCT | 3 cut(s) | 227, 373, 608 |
| AluI | AGCT | 3 cut(s) | 227, 373, 608 |
| Alw26I | GTCTC | 4 cut(s) | 19, 206, 496, 725 |
| AlwI | GGATC | 4 cut(s) | 166, 179, 359, 398 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 368 |
| Aor13HI | TCCGGA | 1 cut(s) | 378 |
| ApeKI | GCWGC | 1 cut(s) | 575 |
| ApoI | RAATTY | 2 cut(s) | 58, 652 |
| AspS9I | GGNCC | 2 cut(s) | 164, 221 |
| AvaII | GGWCC | 2 cut(s) | 164, 221 |
| BamHI | GGATCC | 1 cut(s) | 171 |
| BbsI | GAAGAC | 1 cut(s) | 171 |
| BbvI | GCAGC | 1 cut(s) | 587 |
| BccI | CCATC | 4 cut(s) | 144, 249, 290, 536 |
| BceAI | ACGGC | 3 cut(s) | 542, 614, 701 |
| BciT130I | CCWGG | 2 cut(s) | 129, 527 |
| BclI | TGATCA | 1 cut(s) | 199 |
| BcoDI | GTCTC | 4 cut(s) | 19, 206, 496, 725 |
| BfaI | CTAG | 1 cut(s) | 647 |
| BfmI | CTRYAG | 1 cut(s) | 573 |
| BglII | AGATCT | 1 cut(s) | 71 |
| BisI | GCNGC | 1 cut(s) | 576 |
| BlsI | GCNGC | 1 cut(s) | 577 |
| Bme1390I | CCNGG | 2 cut(s) | 129, 527 |
| Bme18I | GGWCC | 2 cut(s) | 164, 221 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 219 |
| BmgBI | CACGTC | 1 cut(s) | 506 |
| BmgT120I | GGNCC | 2 cut(s) | 164, 221 |
| BmiI | GGNNCC | 2 cut(s) | 173, 689 |
| BmrFI | CCNGG | 2 cut(s) | 129, 527 |
| BmsI | GCATC | 1 cut(s) | 587 |
| BpiI | GAAGAC | 1 cut(s) | 171 |
| BpmI | CTGGAG | 1 cut(s) | 449 |
| Bpu10I | CCTNAGC | 1 cut(s) | 369 |
| BsaI | GGTCTC | 1 cut(s) | 206 |
| BsaJI | CCNNGG | 4 cut(s) | 127, 167, 217, 526 |
| BsaWI | WCCGGW | 1 cut(s) | 378 |
| Bse1I | ACTGG | 1 cut(s) | 247 |
| Bse3DI | GCAATG | 2 cut(s) | 124, 274 |
| BseAI | TCCGGA | 1 cut(s) | 378 |
| BseBI | CCWGG | 2 cut(s) | 129, 527 |
| BseDI | CCNNGG | 4 cut(s) | 127, 167, 217, 526 |
| BseGI | GGATG | 2 cut(s) | 301, 578 |
| BseMI | GCAATG | 2 cut(s) | 124, 274 |
| BseMII | CTCAG | 3 cut(s) | 312, 360, 663 |
| BseNI | ACTGG | 1 cut(s) | 247 |
| BseXI | GCAGC | 1 cut(s) | 587 |
| BseYI | CCCAGC | 1 cut(s) | 582 |
| BsgI | GTGCAG | 1 cut(s) | 652 |
| BsiSI | CCGG | 1 cut(s) | 379 |
| BsmAI | GTCTC | 4 cut(s) | 19, 206, 496, 725 |
| BsmBI | CGTCTC | 1 cut(s) | 496 |
| Bso31I | GGTCTC | 1 cut(s) | 206 |
| Bsp13I | TCCGGA | 1 cut(s) | 378 |
| Bsp143I | GATC | 6 cut(s) | 71, 171, 199, 364, 390, 724 |
| BspCNI | CTCAG | 3 cut(s) | 313, 361, 664 |
| BspEI | TCCGGA | 1 cut(s) | 378 |
| BspHI | TCATGA | 1 cut(s) | 744 |
| BspLI | GGNNCC | 2 cut(s) | 173, 689 |
| BspMAI | CTGCAG | 1 cut(s) | 577 |
| BspPI | GGATC | 4 cut(s) | 166, 179, 359, 398 |
| BspTNI | GGTCTC | 1 cut(s) | 206 |
| BsrDI | GCAATG | 2 cut(s) | 124, 274 |
| BsrI | ACTGG | 1 cut(s) | 247 |
| BssECI | CCNNGG | 4 cut(s) | 127, 167, 217, 526 |
| BssMI | GATC | 6 cut(s) | 71, 171, 199, 364, 390, 724 |
| BssNAI | GTATAC | 1 cut(s) | 327 |
| BssT1I | CCWWGG | 1 cut(s) | 217 |
| Bst1107I | GTATAC | 1 cut(s) | 327 |
| Bst2UI | CCWGG | 2 cut(s) | 129, 527 |
| Bst4CI | ACNGT | 3 cut(s) | 25, 331, 448 |
| Bst6I | CTCTTC | 1 cut(s) | 541 |
| BstDEI | CTNAG | 5 cut(s) | 321, 369, 407, 672, 780 |
| BstF5I | GGATG | 2 cut(s) | 301, 578 |
| BstKTI | GATC | 6 cut(s) | 74, 174, 202, 367, 393, 727 |
| BstMAI | GTCTC | 4 cut(s) | 19, 206, 496, 725 |
| BstMBI | GATC | 6 cut(s) | 71, 171, 199, 364, 390, 724 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 614 |
| BstNI | CCWGG | 2 cut(s) | 129, 527 |
| BstSCI | CCNGG | 2 cut(s) | 127, 525 |
| BstSFI | CTRYAG | 1 cut(s) | 573 |
| BstV1I | GCAGC | 1 cut(s) | 587 |
| BstV2I | GAAGAC | 1 cut(s) | 171 |
| BstX2I | RGATCY | 4 cut(s) | 71, 171, 364, 390 |
| BstYI | RGATCY | 4 cut(s) | 71, 171, 364, 390 |
| BstZ17I | GTATAC | 1 cut(s) | 327 |
| BtrI | CACGTC | 1 cut(s) | 506 |
| BtsCI | GGATG | 2 cut(s) | 301, 578 |
| BtsIMutI | CAGTG | 1 cut(s) | 669 |
| CaiI | CAGNNNCTG | 1 cut(s) | 368 |
| CciI | TCATGA | 1 cut(s) | 744 |
| Cfr13I | GGNCC | 2 cut(s) | 164, 221 |
| Csp6I | GTAC | 1 cut(s) | 531 |
| CviAII | CATG | 3 cut(s) | 659, 728, 745 |
| CviJI | RGCY | 6 cut(s) | 227, 373, 420, 427, 608, 688 |
| CviKI_1 | RGCY | 6 cut(s) | 227, 373, 420, 427, 608, 688 |
| CviQI | GTAC | 1 cut(s) | 531 |
| DdeI | CTNAG | 5 cut(s) | 321, 369, 407, 672, 780 |
| DpnI | GATC | 6 cut(s) | 73, 173, 201, 366, 392, 726 |
| DpnII | GATC | 6 cut(s) | 71, 171, 199, 364, 390, 724 |
| DriI | GACNNNNNGTC | 1 cut(s) | 219 |
| Eam1104I | CTCTTC | 1 cut(s) | 541 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 219 |
| EarI | CTCTTC | 1 cut(s) | 541 |
| Eco130I | CCWWGG | 1 cut(s) | 217 |
| Eco31I | GGTCTC | 1 cut(s) | 206 |
| Eco47I | GGWCC | 2 cut(s) | 164, 221 |
| Eco57I | CTGAAG | 4 cut(s) | 31, 66, 345, 440 |
| EcoRII | CCWGG | 2 cut(s) | 127, 525 |
| EcoT14I | CCWWGG | 1 cut(s) | 217 |
| EcoT22I | ATGCAT | 1 cut(s) | 733 |
| ErhI | CCWWGG | 1 cut(s) | 217 |
| Esp3I | CGTCTC | 1 cut(s) | 496 |
| FaeI | CATG | 3 cut(s) | 662, 731, 748 |
| FatI | CATG | 3 cut(s) | 658, 727, 744 |
| FbaI | TGATCA | 1 cut(s) | 199 |
| FblI | GTMKAC | 1 cut(s) | 326 |
| Fnu4HI | GCNGC | 1 cut(s) | 576 |
| FokI | GGATG | 2 cut(s) | 308, 565 |
| Fsp4HI | GCNGC | 1 cut(s) | 576 |
| FspBI | CTAG | 1 cut(s) | 647 |
| GluI | GCNGC | 1 cut(s) | 576 |
| GsaI | CCCAGC | 1 cut(s) | 586 |
| GsuI | CTGGAG | 1 cut(s) | 449 |
| HapII | CCGG | 1 cut(s) | 379 |
| Hin1II | CATG | 3 cut(s) | 662, 731, 748 |
| HincII | GTYRAC | 1 cut(s) | 124 |
| HindII | GTYRAC | 1 cut(s) | 124 |
| HinfI | GANTC | 3 cut(s) | 132, 663, 718 |
| HpaI | GTTAAC | 1 cut(s) | 124 |
| HpaII | CCGG | 1 cut(s) | 379 |
| Hpy166II | GTNNAC | 2 cut(s) | 124, 327 |
| Hpy188I | TCNGA | 2 cut(s) | 171, 364 |
| Hpy188III | TCNNGA | 9 cut(s) | 203, 293, 320, 352, 368, 379, 707, 722, 745 |
| Hpy8I | GTNNAC | 2 cut(s) | 124, 327 |
| HpyAV | CCTTC | 1 cut(s) | 90 |
| HpyCH4III | ACNGT | 3 cut(s) | 25, 331, 448 |
| HpyCH4IV | ACGT | 1 cut(s) | 505 |
| HpyCH4V | TGCA | 7 cut(s) | 117, 386, 464, 575, 658, 669, 731 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 614 |
| HpyF3I | CTNAG | 5 cut(s) | 321, 369, 407, 672, 780 |
| HpySE526I | ACGT | 1 cut(s) | 505 |
| Hsp92II | CATG | 3 cut(s) | 662, 731, 748 |
| Kpn2I | TCCGGA | 1 cut(s) | 378 |
| Ksp22I | TGATCA | 1 cut(s) | 199 |
| KspAI | GTTAAC | 1 cut(s) | 124 |
| Kzo9I | GATC | 6 cut(s) | 71, 171, 199, 364, 390, 724 |
| LmnI | GCTCC | 1 cut(s) | 693 |
| Lsp1109I | GCAGC | 1 cut(s) | 587 |
| LweI | GCATC | 1 cut(s) | 587 |
| MaeI | CTAG | 1 cut(s) | 647 |
| MaeII | ACGT | 1 cut(s) | 505 |
| MaeIII | GTNAC | 3 cut(s) | 140, 238, 437 |
| MalI | GATC | 6 cut(s) | 73, 173, 201, 366, 392, 726 |
| MboI | GATC | 6 cut(s) | 71, 171, 199, 364, 390, 724 |
| MboII | GAAGA | 2 cut(s) | 171, 558 |
| MfeI | CAATTG | 2 cut(s) | 566, 762 |
| MflI | RGATCY | 4 cut(s) | 71, 171, 364, 390 |
| MluCI | AATT | 8 cut(s) | 28, 58, 347, 552, 566, 612, 652, 762 |
| MlyI | GAGTC | 1 cut(s) | 727 |
| MnlI | CCTC | 5 cut(s) | 19, 107, 177, 346, 667 |
| Mph1103I | ATGCAT | 1 cut(s) | 733 |
| MroI | TCCGGA | 1 cut(s) | 378 |
| MseI | TTAA | 6 cut(s) | 27, 123, 153, 279, 537, 681 |
| MspI | CCGG | 1 cut(s) | 379 |
| MspR9I | CCNGG | 2 cut(s) | 129, 527 |
| MunI | CAATTG | 2 cut(s) | 566, 762 |
| MvaI | CCWGG | 2 cut(s) | 129, 527 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 614 |
| NdeII | GATC | 6 cut(s) | 71, 171, 199, 364, 390, 724 |
| NlaIII | CATG | 3 cut(s) | 662, 731, 748 |
| NlaIV | GGNNCC | 2 cut(s) | 173, 689 |
| NmuCI | GTSAC | 1 cut(s) | 238 |
| NsiI | ATGCAT | 1 cut(s) | 733 |
| PagI | TCATGA | 1 cut(s) | 744 |
| PfeI | GAWTC | 2 cut(s) | 132, 663 |
| PkrI | GCNGC | 1 cut(s) | 577 |
| PleI | GAGTC | 1 cut(s) | 726 |
| PpsI | GAGTC | 1 cut(s) | 726 |
| Psp6I | CCWGG | 2 cut(s) | 127, 525 |
| PspFI | CCCAGC | 1 cut(s) | 582 |
| PspGI | CCWGG | 2 cut(s) | 127, 525 |
| PspN4I | GGNNCC | 2 cut(s) | 173, 689 |
| PspPI | GGNCC | 2 cut(s) | 164, 221 |
| PstI | CTGCAG | 1 cut(s) | 577 |
| PstNI | CAGNNNCTG | 1 cut(s) | 368 |
| PsuI | RGATCY | 4 cut(s) | 71, 171, 364, 390 |
| RsaI | GTAC | 1 cut(s) | 532 |
| RsaNI | GTAC | 1 cut(s) | 531 |
| SaqAI | TTAA | 6 cut(s) | 27, 123, 153, 279, 537, 681 |
| SatI | GCNGC | 1 cut(s) | 576 |
| Sau3AI | GATC | 6 cut(s) | 71, 171, 199, 364, 390, 724 |
| Sau96I | GGNCC | 2 cut(s) | 164, 221 |
| SchI | GAGTC | 1 cut(s) | 727 |
| ScrFI | CCNGG | 2 cut(s) | 129, 527 |
| SetI | ASST | 6 cut(s) | 11, 229, 375, 508, 610, 648 |
| SfaNI | GCATC | 1 cut(s) | 587 |
| SfcI | CTRYAG | 1 cut(s) | 573 |
| SinI | GGWCC | 2 cut(s) | 164, 221 |
| Sse9I | AATT | 8 cut(s) | 28, 58, 347, 552, 566, 612, 652, 762 |
| SspMI | CTAG | 1 cut(s) | 647 |
| StyD4I | CCNGG | 2 cut(s) | 127, 525 |
| StyI | CCWWGG | 1 cut(s) | 217 |
| TaaI | ACNGT | 3 cut(s) | 25, 331, 448 |
| TaiI | ACGT | 1 cut(s) | 508 |
| TaqI | TCGA | 1 cut(s) | 723 |
| TasI | AATT | 8 cut(s) | 28, 58, 347, 552, 566, 612, 652, 762 |
| TfiI | GAWTC | 2 cut(s) | 132, 663 |
| Tru1I | TTAA | 6 cut(s) | 27, 123, 153, 279, 537, 681 |
| Tru9I | TTAA | 6 cut(s) | 27, 123, 153, 279, 537, 681 |
| TscAI | CASTG | 1 cut(s) | 676 |
| TseFI | GTSAC | 1 cut(s) | 238 |
| TseI | GCWGC | 1 cut(s) | 575 |
| Tsp45I | GTSAC | 1 cut(s) | 238 |
| TspDTI | ATGAA | 1 cut(s) | 390 |
| TspRI | CASTG | 1 cut(s) | 676 |
| VpaK11BI | GGWCC | 2 cut(s) | 164, 221 |
| XapI | RAATTY | 2 cut(s) | 58, 652 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 143 |
| XmiI | GTMKAC | 1 cut(s) | 326 |
| XspI | CTAG | 1 cut(s) | 647 |
| Zsp2I | ATGCAT | 1 cut(s) | 733 |