Rw2G019370
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000088
Physical Location & Seq
Forward (+)
25394304 .. 25403108
8805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G019370.1

Sequence Viewer

Length: 3069 bp
ATGTCAGGAAGTGCCTCCCCATCAGTTTTGGTCTTATTTTTGTGCATCTCTGGTGTAGCACAAGCTCAAGTTTCTGCTCCTGCTCCTGCTGCTGCTCCGCAACCTCAAGCTACTAATGCCACCACTGACCCATCTCAAGCAAGAGTTTTGAATTCAGTGTTTGAGAAATGGAATATTCGGGCCGATCCGAAGCAATGGAACACAACCGGAGACCCATGCAGTGGAGCTGCCATAGACTCCACCAGTCACTCCACCAAATTTGATGACCCCGCTTACAACCCCTTCATCATCTGTGACTGCTCTTATCAATCCGGCACCATTTGCCACATTACCCAACTGAAAGTTCAGGACTTGGAAGTTGTTGGTGTAATTCCAGACGAGTTATGGAGTTTGACTTCCCTCACCAATCTGAATTTGGAAAGGAATTCCCTGACAGGACCTCTCTCTGCAGCCATTGCAAATCTAACTGGCTTGCAATACTTTACTATTGGCATCAACGATTTATCAGGCGAGATACCAAAAGAACTTGGACAACTTACTGAACTGAGATCATTGGCTTTTGGGAGAAACAACTTTTCAGGTTCTCTGCCATCTGAGCTAGGGAATTTATTCAAATTACAACAGCTTTACGTTGATAGTTGTGGAGTTAGCGGTGAGATTCCACCAACATTTGCTGATCTACAAAACTTGGAGATTTTTTCGGCTAGAAACGTTGAACTCTCTGGCAGGATACCAGACTTCATAGGGAATTGGTCAAAGCTTACTACCTTGAGGTTTCAAGGAAACTCTTTCGAAGGTCCTATACCATTTGCATTTTCCAATCTGACTTCTGTGAAAGAGCTACGCATTAGTGATTTGTCGAATGCCAATGGGAGCTCTTCTCTTGCATTTATTGAGAATATGAAGGCTCTAACTATCTTAGAGCTGAGGAATAACAATATTTCTGATTCAATTCCTTCCGATTTTGGAGTATACCAAAGTTTGATGCAGCTGGATTTCAGCTTCAACAATTTAACTGGACAGATACCAGACTCACTCTTTAATTTGAGTTCTCTCTCCATCTTGTTTCTTGGAAACAATAAGTTGAATGGCACCCTTCCTGAGTATAAGACAGCACCTCTTCTCAATATAGATTTGTCGTACAATTATCTAGATGGGAGCATTCCTTCTTGGGTCAAAGAACAAAAATTACAGCTTAATTTAGTTGCCAACAACTTCAGCATAGAAAGTTTACAGAGCAGTGGTCTTCCTTCAGGGTTGGCTTGCCTTCAAAGAAACTTTCCTTGCAATCGTGGCCCTGGAATCTATTATAATTTTGGGATTAATTGTGGAGGACCTGAGATTACATCTTCCTCCGATGGGATTGTGTATGAGAAGGAAAATGAGCCCCTTGGACCAGCTTCATATTTTGTGACTGGCACAACCAGATGGGGAGTTAGCAATGTCGGATCTTTCGCTGGCAGTACCAATCACCAGTATACAAGTTTCTCCTTATCTCAATTCACAAATACTTTTGATTCTGTGCTATTCCAGACTGCAAGGCTCTCTGCTTCATCAATTAGATACTTTGGCTTGGGGCTTGAGAATGGTAACTACACCGTGAACCTCAAGTTTGCAGAACATGTCATTCTGGCTGCTACTACCGGGAAATTTCTTGGGAGACGTCTTTTTGATATTTATATCCAGGGCATTCTGGTATCAAAGAATTTTGATATACTAAAGGAGGCGGGTATGTCATCTTTTGGAGCTGTTCAGAAGCCATACACGGCTCATGTTTCGGAGAACTACCTTGAAATCCATCTGTTTTGGGCTGGAAAGGGGACTTGCTGTATACCTGAGCGGGGTACTTATGGACCTGCTATTGCAGCCATCAGTGCTACACCGGATTTCATACCTATTGTCAGTAACAACCCTCCAACTAGTAAGAAGAATAGAACTGGGCCGATTGTTGGGATTGTTGTGGGTGGTGGAGTACTTTTGGTTCTGTTGTTTGTGGCTTTTTATCTTATTCAAAGAAGAAAAAGGTCTGACCCTGATGATGACGAAGAGCTGTATGGGATAGATGTTGGACCACTTACTTTCGGTTATCCTGAACTAAAGACAGCTACAAAGGACTTTGATGAAGCTCATAAGCTTGGAGAGGGAGGATTTGGACCTGTCTATAGAGGAACACTTAGTGATGGAAGAGTAATTGCTGTGAAGCAATTGTCTGCAGCATCCCACCAAGGAAAGAACCAGTTTGTAACTGAGATTGCCACTATATCTGCTGTGCAACACAATAACCTGGTAAAGTTGTACGGATTCTGCACTGAGGGAGTTAAAAGGCTCCTTGTCTATGAGTATCTGGAGAACAACAGTCTTGATCAAGCATTATTTGGAGAGAGATGTTTAAATCTTGATTGGTCAACACGTTTTAATATATGCTTGGGCATAGCTAGAGGTTTAACTTATCTTCATGAAGAATCAAGGGTTCGAATTGTACACAGAGATGTGAAGGCCAGTAATATTCTGCTTGACACTAATCTCATCCCCAAGATATCGGATTTTGGTTTGGCTAAGCTTTTTGATGAGAAAAAGACCCACATAAGTACCCGTGTTGCGGGAACAATTGGGTATCTTGCACCGGAATATGCCATGCGTGGACACCTTACAGAGAAGACTGATGTCTTTGCCTTTGGTGTTCTGGCTCTTGAAATTGTTAGTGGTAGGCCAAATTCTGATCCGAGTTTGGGTGAAGAAATGACTTATCTTCTCGAATGGGCTTGGCAATTGCATGAAAACAGACGGGCAGTTGAACTAGTGGACTCTAGATTATCTGAATTCAATGAGGAAGAAGCACAACGAATAATCAAAATAGGACTTTTGTGCACTCAAACATCGCCAATGCTACGACCATCCATGTCCCGTGTAGTGGGAATGCTTTCAGGAGATATGGAAGTGACAACAGTAACTTCAAAGCCCGGTTACTTGACAGACTGGAAGTTTGATGATTTAACTACAGTCACCAACCTAACAGAACTGGACGGTGTAAGTATTGTCACCAACATGACTGATATATATGACGACTAG

Protein Analysis

1022

Amino Acids

111.88

Weight (kDa)

5.12

Isoelectric Point (pI)

34.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 113 - 257 4.2e-10 Leucine-rich repeat region
LRR_8 PF13855 303 - 362 5.7e-06 Leucine rich repeat
Malectin PF11721 439 - 624 8.2e-39 Malectin domain
Pkinase PF00069 707 - 970 1.9e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 710 - 972 8.9e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1311
AatII GACGTC 1 cut(s) 1666
Acc36I ACCTGC 1 cut(s) 1864
AccB1I GGYRCC 2 cut(s) 314, 1091
AccB7I CCANNNNNTGG 1 cut(s) 221
AccBSI CCGCTC 1 cut(s) 1840
AccI GTMKAC 3 cut(s) 972, 1478, 1831
AciI CCGC 6 cut(s) 98, 270, 651, 1727, 1840, 2600
AclI AACGTT 1 cut(s) 711
AclWI GGATC 3 cut(s) 179, 1456, 2714
AcsI RAATTY 9 cut(s) 151, 257, 412, 424, 604, 1649, 1705, 2713, 2819
AcuI CTGAAG 2 cut(s) 1201, 1236
AcyI GRCGYC 1 cut(s) 1663
AdeI CACNNNGTG 1 cut(s) 2177
AfaI GTAC 7 cut(s) 1142, 1465, 1846, 1974, 2297, 2481, 2590
AfiI CCNNNNNNNGG 7 cut(s) 221, 1359, 1841, 1949, 2599, 2729, 2911
AflIII ACRYGT 2 cut(s) 1621, 2408
AhlI ACTAGT 2 cut(s) 1919, 2797
AjnI CCWGG 3 cut(s) 1297, 1683, 2283
AjuI GAANNNNNNNTTGG 2 cut(s) 327, 359
Alw21I GWGCWC 2 cut(s) 878, 2870
Alw26I GTCTC 2 cut(s) 204, 1654
Alw44I GTGCAC 1 cut(s) 2866
AlwI GGATC 3 cut(s) 179, 1456, 2714
AoxI GGCC 5 cut(s) 180, 1294, 1940, 2496, 2708
ApaLI GTGCAC 1 cut(s) 2866
ApeKI GCWGC 8 cut(s) 89, 92, 227, 449, 988, 1634, 1865, 2213
ApoI RAATTY 9 cut(s) 151, 257, 412, 424, 604, 1649, 1705, 2713, 2819
ArsI GACNNNNNNTTYG 2 cut(s) 2966, 2998
AseI ATTAAT 1 cut(s) 1323
Asp700I GAANNNNTTC 2 cut(s) 608, 2920
AsuC2I CCSGG 2 cut(s) 1645, 2961
AsuHPI GGTGA 6 cut(s) 394, 665, 1463, 2744, 2995, 3031
AsuII TTCGAA 2 cut(s) 792, 2473
AvaII GGWCC 7 cut(s) 437, 797, 1334, 1394, 1853, 2069, 2153
BaeGI GKGCMC 1 cut(s) 2870
BaeI ACNNNNGTAYC 2 cut(s) 2572, 2605
BanI GGYRCC 2 cut(s) 314, 1091
BanII GRGCYC 2 cut(s) 878, 1389
BarI GAAGNNNNNNTAC 2 cut(s) 786, 818
BbsI GAAGAC 2 cut(s) 1238, 2663
Bbv12I GWGCWC 2 cut(s) 878, 2870
BbvCI CCTCAGC 1 cut(s) 926
BbvI GCAGC 8 cut(s) 76, 79, 214, 461, 1000, 1621, 1877, 2225
BceAI ACGGC 1 cut(s) 1782
BciT130I CCWGG 3 cut(s) 1299, 1685, 2285
BciVI GTATCC 1 cut(s) 723
BclI TGATCA 1 cut(s) 2362
BcnI CCSGG 2 cut(s) 1645, 2961
BcoDI GTCTC 2 cut(s) 204, 1654
BcuI ACTAGT 2 cut(s) 1919, 2797
BfaI CTAG 8 cut(s) 599, 705, 1151, 1920, 2436, 2798, 2808, 3067
BfmI CTRYAG 4 cut(s) 447, 2161, 2211, 2997
BfuAI ACCTGC 1 cut(s) 1864
BfuI GTATCC 1 cut(s) 723
BisI GCNGC 8 cut(s) 90, 93, 228, 450, 989, 1635, 1866, 2214
BlpI GCTNAGC 1 cut(s) 2556
BlsI GCNGC 8 cut(s) 91, 94, 229, 451, 990, 1636, 1867, 2215
BmcAI AGTACT 1 cut(s) 1974
Bme1390I CCNGG 5 cut(s) 1299, 1645, 1685, 2285, 2961
Bme18I GGWCC 7 cut(s) 437, 797, 1334, 1394, 1853, 2069, 2153
BmiI GGNNCC 3 cut(s) 316, 1093, 2327
BmrFI CCNGG 5 cut(s) 1299, 1645, 1685, 2285, 2961
BmrI ACTGGG 1 cut(s) 1947
BmsI GCATC 4 cut(s) 54, 501, 975, 2225
BmuI ACTGGG 1 cut(s) 1947
BoxI GACNNNNGTC 1 cut(s) 2663
BpiI GAAGAC 2 cut(s) 1238, 2663
BplI GAGNNNNNCTC 2 cut(s) 865, 897
BpmI CTGGAG 1 cut(s) 2366
Bpu10I CCTNAGC 2 cut(s) 926, 1836
Bpu1102I GCTNAGC 1 cut(s) 2556
Bpu14I TTCGAA 2 cut(s) 792, 2473
BpuEI CTTGAG 6 cut(s) 51, 90, 120, 790, 1592, 1601
BpuMI CCSGG 2 cut(s) 1645, 2961
BsaHI GRCGYC 1 cut(s) 1663
BsaI GGTCTC 1 cut(s) 204
BsaJI CCNNGG 4 cut(s) 1297, 1390, 1684, 2224
BsaWI WCCGGW 3 cut(s) 206, 1882, 2623
Bsc4I CCNNNNNNNGG 7 cut(s) 221, 1359, 1841, 1949, 2599, 2729, 2911
Bse3DI GCAATG 3 cut(s) 200, 453, 1447
BseBI CCWGG 3 cut(s) 1299, 1685, 2285
BseDI CCNNGG 4 cut(s) 1297, 1390, 1684, 2224
BseGI GGATG 3 cut(s) 2216, 2526, 2894
BseLI CCNNNNNNNGG 7 cut(s) 221, 1359, 1841, 1949, 2599, 2729, 2911
BseMI GCAATG 3 cut(s) 200, 453, 1447
BseMII CTCAG 8 cut(s) 536, 585, 917, 1092, 1329, 1827, 2238, 2301
BseSI GKGCMC 1 cut(s) 2870
BseXI GCAGC 8 cut(s) 76, 79, 214, 461, 1000, 1621, 1877, 2225
BsgI GTGCAG 1 cut(s) 2290
BshFI GGCC 5 cut(s) 182, 1296, 1942, 2498, 2710
BshNI GGYRCC 2 cut(s) 314, 1091
BsiHKAI GWGCWC 2 cut(s) 878, 2870
BsiSI CCGG 6 cut(s) 207, 312, 1644, 1883, 2624, 2961
BslFI GGGAC 2 cut(s) 1834, 2887
BslI CCNNNNNNNGG 7 cut(s) 221, 1359, 1841, 1949, 2599, 2729, 2911
BsmAI GTCTC 2 cut(s) 204, 1654
BsmBI CGTCTC 1 cut(s) 1654
BsmFI GGGAC 2 cut(s) 1834, 2887
BsmI GAATGC 4 cut(s) 868, 1161, 1689, 2922
BsnI GGCC 5 cut(s) 182, 1296, 1942, 2498, 2710
Bso31I GGTCTC 1 cut(s) 204
Bsp119I TTCGAA 2 cut(s) 792, 2473
Bsp1286I GDGCHC 3 cut(s) 878, 1389, 2870
Bsp1407I TGTACA 1 cut(s) 2479
Bsp143I GATC 6 cut(s) 184, 548, 676, 1448, 2362, 2719
Bsp1720I GCTNAGC 1 cut(s) 2556
BspACI CCGC 6 cut(s) 98, 270, 651, 1727, 1840, 2600
BspANI GGCC 5 cut(s) 182, 1296, 1942, 2498, 2710
BspCNI CTCAG 8 cut(s) 537, 586, 918, 1093, 1330, 1828, 2239, 2302
BspHI TCATGA 1 cut(s) 2455
BspLI GGNNCC 3 cut(s) 316, 1093, 2327
BspMAI CTGCAG 2 cut(s) 451, 2215
BspMI ACCTGC 1 cut(s) 1864
BspPI GGATC 3 cut(s) 179, 1456, 2714
BspQI GCTCTTC 2 cut(s) 883, 2040
BspT104I TTCGAA 2 cut(s) 792, 2473
BspT107I GGYRCC 2 cut(s) 314, 1091
BspTNI GGTCTC 1 cut(s) 204
BsrBI CCGCTC 1 cut(s) 1840
BsrDI GCAATG 3 cut(s) 200, 453, 1447
BsrGI TGTACA 1 cut(s) 2479
BssECI CCNNGG 4 cut(s) 1297, 1390, 1684, 2224
BssMI GATC 6 cut(s) 184, 548, 676, 1448, 2362, 2719
BssNAI GTATAC 3 cut(s) 973, 1479, 1832
BssNI GRCGYC 1 cut(s) 1663
BssT1I CCWWGG 2 cut(s) 1390, 2224
Bst1107I GTATAC 3 cut(s) 973, 1479, 1832
Bst2UI CCWGG 3 cut(s) 1299, 1685, 2285
Bst4CI ACNGT 5 cut(s) 1600, 2357, 2947, 3001, 3026
Bst6I CTCTTC 4 cut(s) 883, 1125, 2040, 2179
BstACI GRCGYC 1 cut(s) 1663
BstAPI GCANNNNNTGC 2 cut(s) 321, 455
BstAUI TGTACA 1 cut(s) 2479
BstBI TTCGAA 2 cut(s) 792, 2473
BstC8I GCNNGC 3 cut(s) 473, 1264, 1459
BstF5I GGATG 3 cut(s) 2216, 2526, 2894
BstKTI GATC 6 cut(s) 187, 551, 679, 1451, 2365, 2722
BstMAI GTCTC 2 cut(s) 204, 1654
BstMBI GATC 6 cut(s) 184, 548, 676, 1448, 2362, 2719
BstMWI GCNNNNNNNGC 8 cut(s) 89, 116, 321, 455, 595, 1293, 1865, 1874
BstNI CCWGG 3 cut(s) 1299, 1685, 2285
BstNSI RCATGY 1 cut(s) 1625
BstPAI GACNNNNGTC 1 cut(s) 2663
BstSCI CCNGG 5 cut(s) 1297, 1643, 1683, 2283, 2959
BstSFI CTRYAG 4 cut(s) 447, 2161, 2211, 2997
BstSLI GKGCMC 1 cut(s) 2870
BstV1I GCAGC 8 cut(s) 76, 79, 214, 461, 1000, 1621, 1877, 2225
BstV2I GAAGAC 2 cut(s) 1238, 2663
BstX2I RGATCY 1 cut(s) 1448
BstYI RGATCY 1 cut(s) 1448
BstZ17I GTATAC 3 cut(s) 973, 1479, 1832
BsuI GTATCC 1 cut(s) 723
BsuRI GGCC 5 cut(s) 182, 1296, 1942, 2498, 2710
BtgZI GCGATG 1 cut(s) 2862
BtsCI GGATG 3 cut(s) 2216, 2526, 2894
BtsI GCAGTG 2 cut(s) 226, 1246
BtsIMutI CAGTG 6 cut(s) 123, 162, 226, 1246, 1879, 2307
BveI ACCTGC 1 cut(s) 1864
Cac8I GCNNGC 3 cut(s) 473, 1264, 1459
CciI TCATGA 1 cut(s) 2455
CsiI ACCWGGT 1 cut(s) 2283
Csp6I GTAC 7 cut(s) 1141, 1464, 1845, 1973, 2296, 2480, 2589
CspCI CAANNNNNGTGG 2 cut(s) 241, 276
CviAII CATG 8 cut(s) 216, 1622, 1772, 2456, 2635, 2774, 2899, 3046
CviQI GTAC 7 cut(s) 1141, 1464, 1845, 1973, 2296, 2480, 2589
DpnI GATC 6 cut(s) 186, 550, 678, 1450, 2364, 2721
DpnII GATC 6 cut(s) 184, 548, 676, 1448, 2362, 2719
DraI TTTAAA 1 cut(s) 2391
DraIII CACNNNGTG 1 cut(s) 2177
Eam1104I CTCTTC 4 cut(s) 883, 1125, 2040, 2179
EarI CTCTTC 4 cut(s) 883, 1125, 2040, 2179
Ecl136II GAGCTC 1 cut(s) 876
Eco130I CCWWGG 2 cut(s) 1390, 2224
Eco24I GRGCYC 2 cut(s) 878, 1389
Eco31I GGTCTC 1 cut(s) 204
Eco32I GATATC 1 cut(s) 2538
Eco47I GGWCC 7 cut(s) 437, 797, 1334, 1394, 1853, 2069, 2153
Eco53kI GAGCTC 1 cut(s) 876
Eco57I CTGAAG 2 cut(s) 1201, 1236
EcoICRI GAGCTC 1 cut(s) 876
EcoO109I RGGNCCY 3 cut(s) 437, 797, 1334
EcoRI GAATTC 3 cut(s) 151, 424, 2819
EcoRII CCWGG 3 cut(s) 1297, 1683, 2283
EcoRV GATATC 1 cut(s) 2538
EcoT14I CCWWGG 2 cut(s) 1390, 2224
EcoT38I GRGCYC 2 cut(s) 878, 1389
ErhI CCWWGG 2 cut(s) 1390, 2224
Esp3I CGTCTC 1 cut(s) 1654
FaeI CATG 8 cut(s) 219, 1625, 1775, 2459, 2638, 2777, 2902, 3049
FaqI GGGAC 2 cut(s) 1834, 2887
FatI CATG 8 cut(s) 215, 1621, 1771, 2455, 2634, 2773, 2898, 3045
FauI CCCGC 4 cut(s) 277, 1720, 1833, 2593
FbaI TGATCA 1 cut(s) 2362
FblI GTMKAC 3 cut(s) 972, 1478, 1831
Fnu4HI GCNGC 8 cut(s) 90, 93, 228, 450, 989, 1635, 1866, 2214
FokI GGATG 3 cut(s) 2203, 2513, 2881
FriOI GRGCYC 2 cut(s) 878, 1389
Fsp4HI GCNGC 8 cut(s) 90, 93, 228, 450, 989, 1635, 1866, 2214
FspBI CTAG 8 cut(s) 599, 705, 1151, 1920, 2436, 2798, 2808, 3067
GluI GCNGC 8 cut(s) 90, 93, 228, 450, 989, 1635, 1866, 2214
GsuI CTGGAG 1 cut(s) 2366
HaeIII GGCC 5 cut(s) 182, 1296, 1942, 2498, 2710
HapII CCGG 6 cut(s) 207, 312, 1644, 1883, 2624, 2961
Hin1I GRCGYC 1 cut(s) 1663
Hin1II CATG 8 cut(s) 219, 1625, 1775, 2459, 2638, 2777, 2902, 3049
HincII GTYRAC 1 cut(s) 2406
HindII GTYRAC 1 cut(s) 2406
HindIII AAGCTT 3 cut(s) 758, 2132, 2558
HinfI GANTC 9 cut(s) 236, 658, 947, 1031, 1302, 1517, 2301, 2462, 2804
HpaII CCGG 6 cut(s) 207, 312, 1644, 1883, 2624, 2961
HphI GGTGA 6 cut(s) 394, 665, 1463, 2744, 2995, 3031
HpyCH4III ACNGT 5 cut(s) 1600, 2357, 2947, 3001, 3026
HpyCH4IV ACGT 4 cut(s) 630, 711, 1663, 2410
HpyF10VI GCNNNNNNNGC 8 cut(s) 89, 116, 321, 455, 595, 1293, 1865, 1874
HpySE526I ACGT 4 cut(s) 630, 711, 1663, 2410
Hsp92I GRCGYC 1 cut(s) 1663
Hsp92II CATG 8 cut(s) 219, 1625, 1775, 2459, 2638, 2777, 2902, 3049
Ksp22I TGATCA 1 cut(s) 2362
Kzo9I GATC 6 cut(s) 184, 548, 676, 1448, 2362, 2719
LguI GCTCTTC 2 cut(s) 883, 2040
LmnI GCTCC 8 cut(s) 82, 88, 100, 224, 873, 1158, 1745, 2331
Lsp1109I GCAGC 8 cut(s) 76, 79, 214, 461, 1000, 1621, 1877, 2225
LweI GCATC 4 cut(s) 54, 501, 975, 2225
MabI ACCWGGT 1 cut(s) 2283
MaeI CTAG 8 cut(s) 599, 705, 1151, 1920, 2436, 2798, 2808, 3067
MaeII ACGT 4 cut(s) 630, 711, 1663, 2410
MalI GATC 6 cut(s) 186, 550, 678, 1450, 2364, 2721
MbiI CCGCTC 1 cut(s) 1840
MboI GATC 6 cut(s) 184, 548, 676, 1448, 2362, 2719
MfeI CAATTG 3 cut(s) 2204, 2607, 2768
MflI RGATCY 1 cut(s) 1448
MhlI GDGCHC 3 cut(s) 878, 1389, 2870
MlyI GAGTC 3 cut(s) 230, 1025, 2798
MmeI TCCRAC 3 cut(s) 1426, 1940, 2047
MroXI GAANNNNTTC 2 cut(s) 608, 2920
MseI TTAA 9 cut(s) 1013, 1041, 1197, 1323, 2319, 2390, 2415, 2444, 2993
MslI CAYNNNNRTG 2 cut(s) 2487, 3044
MspA1I CMGCKG 1 cut(s) 991
MspI CCGG 6 cut(s) 207, 312, 1644, 1883, 2624, 2961
MspR9I CCNGG 5 cut(s) 1299, 1645, 1685, 2285, 2961
MunI CAATTG 3 cut(s) 2204, 2607, 2768
Mva1269I GAATGC 4 cut(s) 868, 1161, 1689, 2922
MvaI CCWGG 3 cut(s) 1299, 1685, 2285
MwoI GCNNNNNNNGC 8 cut(s) 89, 116, 321, 455, 595, 1293, 1865, 1874
NciI CCSGG 2 cut(s) 1645, 2961
NdeII GATC 6 cut(s) 184, 548, 676, 1448, 2362, 2719
NlaIII CATG 8 cut(s) 219, 1625, 1775, 2459, 2638, 2777, 2902, 3049
NlaIV GGNNCC 3 cut(s) 316, 1093, 2327
NmuCI GTSAC 6 cut(s) 245, 293, 1411, 2938, 3001, 3037
NspI RCATGY 1 cut(s) 1625
NspV TTCGAA 2 cut(s) 792, 2473
PagI TCATGA 1 cut(s) 2455
PciI ACATGT 1 cut(s) 1621
PciSI GCTCTTC 2 cut(s) 883, 2040
PctI GAATGC 4 cut(s) 868, 1161, 1689, 2922
PdmI GAANNNNTTC 2 cut(s) 608, 2920
PfeI GAWTC 6 cut(s) 658, 947, 1302, 1517, 2301, 2462
PflMI CCANNNNNTGG 1 cut(s) 221
PkrI GCNGC 8 cut(s) 91, 94, 229, 451, 990, 1636, 1867, 2215
PleI GAGTC 3 cut(s) 230, 1025, 2798
PpsI GAGTC 3 cut(s) 230, 1025, 2798
PpuMI RGGWCCY 3 cut(s) 437, 797, 1334
PscI ACATGT 1 cut(s) 1621
PshAI GACNNNNGTC 1 cut(s) 2663
PshBI ATTAAT 1 cut(s) 1323
PsiI TTATAA 1 cut(s) 1311
Psp124BI GAGCTC 1 cut(s) 878
Psp1406I AACGTT 1 cut(s) 711
Psp5II RGGWCCY 3 cut(s) 437, 797, 1334
Psp6I CCWGG 3 cut(s) 1297, 1683, 2283
PspGI CCWGG 3 cut(s) 1297, 1683, 2283
PspN4I GGNNCC 3 cut(s) 316, 1093, 2327
PspPPI RGGWCCY 3 cut(s) 437, 797, 1334
PsrI GAACNNNNNNTAC 4 cut(s) 1173, 1205, 1965, 1997
PstI CTGCAG 2 cut(s) 451, 2215
PsuI RGATCY 1 cut(s) 1448
PvuII CAGCTG 1 cut(s) 991
RsaI GTAC 7 cut(s) 1142, 1465, 1846, 1974, 2297, 2481, 2590
RsaNI GTAC 7 cut(s) 1141, 1464, 1845, 1973, 2296, 2480, 2589
RseI CAYNNNNRTG 2 cut(s) 2487, 3044
SacI GAGCTC 1 cut(s) 878
SapI GCTCTTC 2 cut(s) 883, 2040
SaqAI TTAA 9 cut(s) 1013, 1041, 1197, 1323, 2319, 2390, 2415, 2444, 2993
SatI GCNGC 8 cut(s) 90, 93, 228, 450, 989, 1635, 1866, 2214
Sau3AI GATC 6 cut(s) 184, 548, 676, 1448, 2362, 2719
ScaI AGTACT 1 cut(s) 1974
SchI GAGTC 3 cut(s) 230, 1025, 2798
ScrFI CCNGG 5 cut(s) 1299, 1645, 1685, 2285, 2961
SduI GDGCHC 3 cut(s) 878, 1389, 2870
SexAI ACCWGGT 1 cut(s) 2283
SfaNI GCATC 4 cut(s) 54, 501, 975, 2225
SfcI CTRYAG 4 cut(s) 447, 2161, 2211, 2997
SfuI TTCGAA 2 cut(s) 792, 2473
SinI GGWCC 7 cut(s) 437, 797, 1334, 1394, 1853, 2069, 2153
SmiMI CAYNNNNRTG 2 cut(s) 2487, 3044
SmlI CTYRAG 6 cut(s) 66, 105, 135, 769, 1580, 1607
SmoI CTYRAG 6 cut(s) 66, 105, 135, 769, 1580, 1607
SpeI ACTAGT 2 cut(s) 1919, 2797
SsiI CCGC 6 cut(s) 98, 270, 651, 1727, 1840, 2600
SspI AATATT 3 cut(s) 175, 940, 2506
SspMI CTAG 8 cut(s) 599, 705, 1151, 1920, 2436, 2798, 2808, 3067
SstI GAGCTC 1 cut(s) 878
StyD4I CCNGG 5 cut(s) 1297, 1643, 1683, 2283, 2959
StyI CCWWGG 2 cut(s) 1390, 2224
TaaI ACNGT 5 cut(s) 1600, 2357, 2947, 3001, 3026
TaiI ACGT 4 cut(s) 633, 714, 1666, 2413
TaqI TCGA 4 cut(s) 792, 860, 2473, 2754
TatI WGTACW 2 cut(s) 1972, 2479
TfiI GAWTC 6 cut(s) 658, 947, 1302, 1517, 2301, 2462
Tru1I TTAA 9 cut(s) 1013, 1041, 1197, 1323, 2319, 2390, 2415, 2444, 2993
Tru9I TTAA 9 cut(s) 1013, 1041, 1197, 1323, 2319, 2390, 2415, 2444, 2993
TscAI CASTG 6 cut(s) 130, 162, 226, 1246, 1879, 2314
TseFI GTSAC 6 cut(s) 245, 293, 1411, 2938, 3001, 3037
TseI GCWGC 8 cut(s) 89, 92, 227, 449, 988, 1634, 1865, 2213
Tsp45I GTSAC 6 cut(s) 245, 293, 1411, 2938, 3001, 3037
TspGWI ACGGA 1 cut(s) 2313
TspRI CASTG 6 cut(s) 130, 162, 226, 1246, 1879, 2314
Van91I CCANNNNNTGG 1 cut(s) 221
VneI GTGCAC 1 cut(s) 2866
VpaK11BI GGWCC 7 cut(s) 437, 797, 1334, 1394, 1853, 2069, 2153
VspI ATTAAT 1 cut(s) 1323
XapI RAATTY 9 cut(s) 151, 257, 412, 424, 604, 1649, 1705, 2713, 2819
XbaI TCTAGA 2 cut(s) 1150, 2807
XceI RCATGY 1 cut(s) 1625
XcmI CCANNNNNNNNNTGG 2 cut(s) 381, 412
XmiI GTMKAC 3 cut(s) 972, 1478, 1831
XmnI GAANNNNTTC 2 cut(s) 608, 2920
XspI CTAG 8 cut(s) 599, 705, 1151, 1920, 2436, 2798, 2808, 3067
ZraI GACGTC 1 cut(s) 1664
ZrmI AGTACT 1 cut(s) 1974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.