RchiOBHm_Chr2g0114711
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000088
Physical Location & Seq
Forward (+)
26564614 .. 26574295
9682 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48792

Sequence Viewer

Length: 2925 bp
ATGTTTGGCGCAGTCTTTTGGGTTGCAAGAGTTTTGAATTCAGTGTTTGAGAAATGGAAGATTCGGGCCGATCCAAAGCAATGGAACACAACCGGAGACCCATGCAGTGGAGCTGCCATAGACTCCACCAGTCACTCCACCAAATTTGATGACCCCGCTTACAACCCCTTTATCATATGTGACTGCTCTTATCAATCCGGCACCATTTGCCACATTACCCAACTGAAAGTTCAGAACTTGGGAGTTGTTGGTGTAATTCCAGACGAGTTATGGAGTTTGACTTCCCTCACCAATCTGTATGCTTTCTCTGAACTCCTGTACTTGAGTTGGACTCAGTTTAGTTACCCAATTTGTGATATTTCTCCCATGTATTGTAGGAATTTGGAAAGGAATTCCCTGACAGGACCTCTCTCTGCAGCCATTGCAAATCTAACTGGCTTGCAATACTTTACTATTGGCATCAACGATTTATCAGGCGAGATACCAAAAGAACTTGGACAACTTACTGAACTGAGATCATTGGCTTTTGGGAGAAACAACTTTTCGGGTTCTCTGCCATCTGAGCTAGGGAATTTATTCAAATTACAACAGCTTTACGTTGATAGTTGTGGAGTTAGCGGTGAGATTCCACCAACATTTGCTGATCTACAAAACTTGGAGATTTTTTCGGCTAGAAACGTTGAACTCTCAGGCAGGATACCAGACTTCGTAGGGAATTGGTCAAAGCTTACTACCTTGAGGTTTCAAGGAAACTCTTTCGAAGGTCCTATACCATTTGCATTTTCCAATCTGACTTCTATGAAAGAGCTACGCATTAGTGATTTGTCGAATGCCAATGGGAGCTCTTCTCTTGCATTTATTGAGAATATGAAGGCTCTAAATATCTTAGAGCTGAGGAATAACAATATTTCTGATTCAATTCCTTCCGATTTTGGAGTATACCAAAGTTTGTCGCAGCTGGATTTGAGCTTCAACAATTTAACTGGACAGATACCAGACTCACTCTTTAATTTGAGTTCTCTCTCCATCTTAGATTTGTCGTACAATTATCTAGATGGGAGCATTCCTTCTTGGGTCAAAGAAGAAAAATTACAGCTTGGTCTTCCTTCAGGGTTGGCTTGCCTTCAAAGAAACTTTCCTTGCAATCGTGGCCCTGGAATCTATTATAATTTTGGGATTAATTGTGGAGGACCTGAGATTACGTCTTCGTCCGATGGGATTGTGTATGAGAAGGAAAATGAGCCCCTTGGACCAGCTTCATATTTTGTGACTGGCACAACCAGATGGGGAGTTAGCAATGTCGGATATTTCGCTGGCAGTACCAATGCTCAGTATACAAGTTTCTCCTTATCTCAATTCACAAATACTTTTGATTCTGTGCTATTCCAGACTGCAAGGCTCTCTGCTTCATCAATTAGATACTTTGGCTTGGGGCTTGAGAATGGTAAATACACCGTGAACCTCAAGTTTGCAGAACATGTCATTCTGGCTGCTACTACCGGGAAATTTCTTGGGAGACGTCTTTTTGATATTTATATCCAGGGCATTCTGGTATCAAAGGATTTTGATATACTAAAGGAGGCAGGTATGTCATCTTTCGGAGCTGTTCAGAAGCCATACACGGCTCGTGTTTCGGAGAACTACCTTGAAATCCATCTGTTTTGGGCTGGAAAGGGGACTTGCTGTATACCTGAGCGGGGTACTTATGGACCTGCTATTGCAGCCATCAGTGCTACACCGGATTTCATACCTACTGTCAGTAACAGCCTTCCAACTAGTAAGAAGAATAGAACTGGGCTGATTGTTGGGATTGTTGTGGGTGGTGGAGTACTTTTGGTTCTGTTGTTTGTGGCTTTTTATCTTATTCAAAGAAGAAAAAGGTCTGACCCTGATGATGACGAAGAGCGGTATGGGATAGATGTTGGACCACTTACTTTTGGTTATCCTGAACTAAAGACAGCTACAAATGACTTTGATGAAGCTCATAAGCTTGGAGAGGGAGGATTTGGACCTGTCTATAGAGGAACACTTAGTGATGGAAGAGTAATTGCTGTGAAGCAATTGTCTGCAGCATCCCACCAAGGAAAGAACCAGTTTGTAACTGAGATTGCCACTATATCTGCTGTGCAACACAATAACCTGGTAAAGTTGTACGGATTCTGCACTGAGGGTGTTAAAAGGCTCCTTGTCTATGAGTATCTGGAGAACAACAGTCTTGATCAAGCATTATTTGGAGAGAGATGTTTAAATCTTGATTGGTCAACACGTTTTAATATATGCTTGGGCATAGCTAGAGGTTTAACTTATCTTCATGAAGAATCAAGGGTTCGAATTGTACACAGAGATGTGAAGGCCAGTAATATTCTGCTTGACACTAATCTCATCCCCAAGATATCGGATTTTGGTTTGGCTAAGCTTTTTGATGAGAAAAAGACCCACATAAGTACCCATGTTGCGGGAACAATTGGGTATCTTGCACCTGAATATGCCATGCGTGGACACCTTACAGAGAAGACTGATGTCTTTGCCTTTGGTGTTCTGGCTCTTGAAATTGTTAGTGGTAGGCCAAATTCTGATCCGAGTTTGGGTGAAGAAATGACTTATCTTCTCGAATGGGCTTGGCAATTGCATGAAAACAGACGGGCAGTTGAACTAGTGGACTCTAGATTATCTGAATTCAATGAGGAAGAAGCACAACGAATAATCAAAATAGGACTTTTGTGCACTCAAACATCACCAATGCTACGACCATCCATGTCCCGTGTAGTGGGAATGCTTTCAGGAGATATGGAAGTGACAACAGTAACTTCAAAGCCCGGTTACTTGACAGACTGGAAGTTTGATGATTTAACTACAGTCACCAGCCTAACAGAACTGGACGGTGTAAGTATTGTCACCAACATGACTGATATATATGACGACTAG

Protein Analysis

974

Amino Acids

107.57

Weight (kDa)

5.16

Isoelectric Point (pI)

31.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 139 - 247 6.1e-09 Leucine-rich repeat region
LRR_14 PF23598 262 - 348 5.3e-06 Leucine-rich repeat region
Malectin PF11721 391 - 576 3.3e-38 Malectin domain
Pkinase PF00069 659 - 922 9.6e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 662 - 924 5.9e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1167
AatII GACGTC 1 cut(s) 1522
Acc36I ACCTGC 2 cut(s) 1574, 1720
AccB1I GGYRCC 1 cut(s) 200
AccB7I CCANNNNNTGG 1 cut(s) 107
AccBSI CCGCTC 2 cut(s) 1696, 1906
AccI GTMKAC 3 cut(s) 939, 1334, 1687
AciI CCGC 5 cut(s) 156, 618, 1696, 1906, 2456
AclI AACGTT 1 cut(s) 678
AclWI GGATC 2 cut(s) 65, 2570
AcsI RAATTY 8 cut(s) 37, 143, 379, 391, 571, 1505, 2569, 2675
AcuI CTGAAG 1 cut(s) 1092
AcyI GRCGYC 1 cut(s) 1519
AdeI CACNNNGTG 1 cut(s) 2033
AfaI GTAC 8 cut(s) 320, 1043, 1321, 1702, 1830, 2153, 2337, 2446
AfiI CCNNNNNNNGG 5 cut(s) 107, 1697, 2455, 2585, 2767
AflIII ACRYGT 2 cut(s) 1477, 2264
AhlI ACTAGT 2 cut(s) 1775, 2653
AjnI CCWGG 3 cut(s) 1153, 1539, 2139
AjuI GAANNNNNNNTTGG 2 cut(s) 213, 245
Alw21I GWGCWC 2 cut(s) 845, 2726
Alw26I GTCTC 2 cut(s) 90, 1510
Alw44I GTGCAC 1 cut(s) 2722
AlwI GGATC 2 cut(s) 65, 2570
AoxI GGCC 4 cut(s) 66, 1150, 2352, 2564
ApaLI GTGCAC 1 cut(s) 2722
ApeKI GCWGC 6 cut(s) 113, 416, 955, 1490, 1721, 2069
ApoI RAATTY 8 cut(s) 37, 143, 379, 391, 571, 1505, 2569, 2675
ArsI GACNNNNNNTTYG 2 cut(s) 2822, 2854
AseI ATTAAT 1 cut(s) 1179
Asp700I GAANNNNTTC 2 cut(s) 575, 2776
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 9 cut(s) 66, 404, 764, 1151, 1190, 1250, 1709, 1925, 2009
AsuC2I CCSGG 2 cut(s) 1501, 2817
AsuHPI GGTGA 6 cut(s) 280, 632, 2600, 2727, 2851, 2887
AsuII TTCGAA 2 cut(s) 759, 2329
AvaII GGWCC 7 cut(s) 404, 764, 1190, 1250, 1709, 1925, 2009
BaeGI GKGCMC 1 cut(s) 2726
BaeI ACNNNNGTAYC 2 cut(s) 2428, 2461
BanI GGYRCC 1 cut(s) 200
BanII GRGCYC 2 cut(s) 845, 1245
BarI GAAGNNNNNNTAC 4 cut(s) 753, 785, 1074, 1106
BauI CACGAG 1 cut(s) 1626
BbsI GAAGAC 3 cut(s) 1094, 1197, 2519
Bbv12I GWGCWC 2 cut(s) 845, 2726
BbvCI CCTCAGC 1 cut(s) 893
BbvI GCAGC 6 cut(s) 100, 428, 967, 1477, 1733, 2081
BccI CCATC 9 cut(s) 565, 1034, 1049, 1208, 1278, 1662, 1733, 2030, 2758
BceAI ACGGC 1 cut(s) 1638
BciT130I CCWGG 3 cut(s) 1155, 1541, 2141
BciVI GTATCC 1 cut(s) 690
BclI TGATCA 1 cut(s) 2218
BcnI CCSGG 2 cut(s) 1501, 2817
BcoDI GTCTC 2 cut(s) 90, 1510
BcuI ACTAGT 2 cut(s) 1775, 2653
BfaI CTAG 8 cut(s) 566, 672, 1052, 1776, 2292, 2654, 2664, 2923
BfmI CTRYAG 4 cut(s) 414, 2017, 2067, 2853
BfuAI ACCTGC 2 cut(s) 1574, 1720
BfuI GTATCC 1 cut(s) 690
BisI GCNGC 6 cut(s) 114, 417, 956, 1491, 1722, 2070
BlpI GCTNAGC 1 cut(s) 2412
BlsI GCNGC 6 cut(s) 115, 418, 957, 1492, 1723, 2071
BmcAI AGTACT 1 cut(s) 1830
Bme1390I CCNGG 5 cut(s) 1155, 1501, 1541, 2141, 2817
Bme18I GGWCC 7 cut(s) 404, 764, 1190, 1250, 1709, 1925, 2009
BmgT120I GGNCC 9 cut(s) 66, 404, 764, 1151, 1190, 1250, 1709, 1925, 2009
BmiI GGNNCC 2 cut(s) 202, 2183
BmrFI CCNGG 5 cut(s) 1155, 1501, 1541, 2141, 2817
BmrI ACTGGG 1 cut(s) 1803
BmsI GCATC 2 cut(s) 468, 2081
BmuI ACTGGG 1 cut(s) 1803
BoxI GACNNNNGTC 1 cut(s) 2519
BpiI GAAGAC 3 cut(s) 1094, 1197, 2519
BplI GAGNNNNNCTC 4 cut(s) 316, 348, 832, 864
BpmI CTGGAG 1 cut(s) 2222
Bpu10I CCTNAGC 2 cut(s) 893, 1692
Bpu1102I GCTNAGC 1 cut(s) 2412
Bpu14I TTCGAA 2 cut(s) 759, 2329
BpuEI CTTGAG 4 cut(s) 343, 757, 1448, 1457
BpuMI CCSGG 2 cut(s) 1501, 2817
BsaHI GRCGYC 1 cut(s) 1519
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 4 cut(s) 1153, 1246, 1540, 2080
BsaWI WCCGGW 2 cut(s) 92, 1738
Bsc4I CCNNNNNNNGG 5 cut(s) 107, 1697, 2455, 2585, 2767
Bse1I ACTGG 9 cut(s) 129, 439, 988, 1276, 1798, 2092, 2355, 2837, 2880
Bse3DI GCAATG 3 cut(s) 86, 420, 1303
BseBI CCWGG 3 cut(s) 1155, 1541, 2141
BseDI CCNNGG 4 cut(s) 1153, 1246, 1540, 2080
BseGI GGATG 3 cut(s) 2072, 2382, 2750
BseLI CCNNNNNNNGG 5 cut(s) 107, 1697, 2455, 2585, 2767
BseMI GCAATG 3 cut(s) 86, 420, 1303
BseNI ACTGG 9 cut(s) 129, 439, 988, 1276, 1798, 2092, 2355, 2837, 2880
BseSI GKGCMC 1 cut(s) 2726
BseXI GCAGC 6 cut(s) 100, 428, 967, 1477, 1733, 2081
BsgI GTGCAG 1 cut(s) 2146
BshFI GGCC 4 cut(s) 68, 1152, 2354, 2566
BshNI GGYRCC 1 cut(s) 200
BsiHKAI GWGCWC 2 cut(s) 845, 2726
BsiSI CCGG 5 cut(s) 93, 198, 1500, 1739, 2817
BslFI GGGAC 2 cut(s) 1690, 2743
BslI CCNNNNNNNGG 5 cut(s) 107, 1697, 2455, 2585, 2767
BsmAI GTCTC 2 cut(s) 90, 1510
BsmBI CGTCTC 1 cut(s) 1510
BsmFI GGGAC 2 cut(s) 1690, 2743
BsmI GAATGC 4 cut(s) 835, 1062, 1545, 2778
BsnI GGCC 4 cut(s) 68, 1152, 2354, 2566
Bso31I GGTCTC 1 cut(s) 90
Bsp119I TTCGAA 2 cut(s) 759, 2329
Bsp1286I GDGCHC 3 cut(s) 845, 1245, 2726
Bsp1407I TGTACA 1 cut(s) 2335
Bsp143I GATC 5 cut(s) 70, 515, 643, 2218, 2575
Bsp1720I GCTNAGC 1 cut(s) 2412
BspACI CCGC 5 cut(s) 156, 618, 1696, 1906, 2456
BspANI GGCC 4 cut(s) 68, 1152, 2354, 2566
BspHI TCATGA 1 cut(s) 2311
BspLI GGNNCC 2 cut(s) 202, 2183
BspMAI CTGCAG 2 cut(s) 418, 2071
BspMI ACCTGC 2 cut(s) 1574, 1720
BspPI GGATC 2 cut(s) 65, 2570
BspQI GCTCTTC 2 cut(s) 850, 1896
BspT104I TTCGAA 2 cut(s) 759, 2329
BspT107I GGYRCC 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 90
BsrBI CCGCTC 2 cut(s) 1696, 1906
BsrDI GCAATG 3 cut(s) 86, 420, 1303
BsrGI TGTACA 1 cut(s) 2335
BsrI ACTGG 9 cut(s) 129, 439, 988, 1276, 1798, 2092, 2355, 2837, 2880
BssECI CCNNGG 4 cut(s) 1153, 1246, 1540, 2080
BssMI GATC 5 cut(s) 70, 515, 643, 2218, 2575
BssNAI GTATAC 3 cut(s) 940, 1335, 1688
BssNI GRCGYC 1 cut(s) 1519
BssSI CACGAG 1 cut(s) 1626
BssT1I CCWWGG 2 cut(s) 1246, 2080
Bst1107I GTATAC 3 cut(s) 940, 1335, 1688
Bst2BI CACGAG 1 cut(s) 1626
Bst2UI CCWGG 3 cut(s) 1155, 1541, 2141
Bst4CI ACNGT 6 cut(s) 1456, 1756, 2213, 2803, 2857, 2882
Bst6I CTCTTC 3 cut(s) 850, 1896, 2035
BstACI GRCGYC 1 cut(s) 1519
BstAPI GCANNNNNTGC 2 cut(s) 207, 422
BstAUI TGTACA 1 cut(s) 2335
BstBI TTCGAA 2 cut(s) 759, 2329
BstC8I GCNNGC 3 cut(s) 440, 1120, 1315
BstF5I GGATG 3 cut(s) 2072, 2382, 2750
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 5 cut(s) 73, 518, 646, 2221, 2578
BstMAI GTCTC 2 cut(s) 90, 1510
BstMBI GATC 5 cut(s) 70, 515, 643, 2218, 2575
BstMWI GCNNNNNNNGC 6 cut(s) 207, 422, 562, 1149, 1721, 1730
BstNI CCWGG 3 cut(s) 1155, 1541, 2141
BstNSI RCATGY 1 cut(s) 1481
BstPAI GACNNNNGTC 1 cut(s) 2519
BstSCI CCNGG 5 cut(s) 1153, 1499, 1539, 2139, 2815
BstSFI CTRYAG 4 cut(s) 414, 2017, 2067, 2853
BstSLI GKGCMC 1 cut(s) 2726
BstV1I GCAGC 6 cut(s) 100, 428, 967, 1477, 1733, 2081
BstV2I GAAGAC 3 cut(s) 1094, 1197, 2519
BstXI CCANNNNNNTGG 1 cut(s) 81
BstZ17I GTATAC 3 cut(s) 940, 1335, 1688
BsuI GTATCC 1 cut(s) 690
BsuRI GGCC 4 cut(s) 68, 1152, 2354, 2566
BtsCI GGATG 3 cut(s) 2072, 2382, 2750
BtsI GCAGTG 1 cut(s) 112
BtsIMutI CAGTG 4 cut(s) 48, 112, 1735, 2163
BveI ACCTGC 2 cut(s) 1574, 1720
Cac8I GCNNGC 3 cut(s) 440, 1120, 1315
CciI TCATGA 1 cut(s) 2311
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 9 cut(s) 66, 404, 764, 1151, 1190, 1250, 1709, 1925, 2009
CsiI ACCWGGT 1 cut(s) 2139
Csp6I GTAC 8 cut(s) 319, 1042, 1320, 1701, 1829, 2152, 2336, 2445
CspCI CAANNNNNGTGG 2 cut(s) 127, 162
CviAII CATG 9 cut(s) 102, 367, 1478, 2312, 2450, 2491, 2630, 2755, 2902
CviQI GTAC 8 cut(s) 319, 1042, 1320, 1701, 1829, 2152, 2336, 2445
DpnI GATC 5 cut(s) 72, 517, 645, 2220, 2577
DpnII GATC 5 cut(s) 70, 515, 643, 2218, 2575
DraI TTTAAA 1 cut(s) 2247
DraIII CACNNNGTG 1 cut(s) 2033
Eam1104I CTCTTC 3 cut(s) 850, 1896, 2035
EarI CTCTTC 3 cut(s) 850, 1896, 2035
Ecl136II GAGCTC 1 cut(s) 843
Eco130I CCWWGG 2 cut(s) 1246, 2080
Eco24I GRGCYC 2 cut(s) 845, 1245
Eco31I GGTCTC 1 cut(s) 90
Eco32I GATATC 1 cut(s) 2394
Eco47I GGWCC 7 cut(s) 404, 764, 1190, 1250, 1709, 1925, 2009
Eco53kI GAGCTC 1 cut(s) 843
Eco57I CTGAAG 1 cut(s) 1092
EcoICRI GAGCTC 1 cut(s) 843
EcoO109I RGGNCCY 3 cut(s) 404, 764, 1190
EcoRI GAATTC 3 cut(s) 37, 391, 2675
EcoRII CCWGG 3 cut(s) 1153, 1539, 2139
EcoRV GATATC 1 cut(s) 2394
EcoT14I CCWWGG 2 cut(s) 1246, 2080
EcoT38I GRGCYC 2 cut(s) 845, 1245
ErhI CCWWGG 2 cut(s) 1246, 2080
Esp3I CGTCTC 1 cut(s) 1510
FaeI CATG 9 cut(s) 105, 370, 1481, 2315, 2453, 2494, 2633, 2758, 2905
FaqI GGGAC 2 cut(s) 1690, 2743
FatI CATG 9 cut(s) 101, 366, 1477, 2311, 2449, 2490, 2629, 2754, 2901
FauI CCCGC 3 cut(s) 163, 1689, 2449
FauNDI CATATG 1 cut(s) 176
FbaI TGATCA 1 cut(s) 2218
FblI GTMKAC 3 cut(s) 939, 1334, 1687
Fnu4HI GCNGC 6 cut(s) 114, 417, 956, 1491, 1722, 2070
FokI GGATG 3 cut(s) 2059, 2369, 2737
FriOI GRGCYC 2 cut(s) 845, 1245
Fsp4HI GCNGC 6 cut(s) 114, 417, 956, 1491, 1722, 2070
FspBI CTAG 8 cut(s) 566, 672, 1052, 1776, 2292, 2654, 2664, 2923
GlaI GCGC 1 cut(s) 10
GluI GCNGC 6 cut(s) 114, 417, 956, 1491, 1722, 2070
GsuI CTGGAG 1 cut(s) 2222
HaeIII GGCC 4 cut(s) 68, 1152, 2354, 2566
HapII CCGG 5 cut(s) 93, 198, 1500, 1739, 2817
HhaI GCGC 1 cut(s) 11
Hin1I GRCGYC 1 cut(s) 1519
Hin1II CATG 9 cut(s) 105, 370, 1481, 2315, 2453, 2494, 2633, 2758, 2905
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HincII GTYRAC 1 cut(s) 2262
HindII GTYRAC 1 cut(s) 2262
HindIII AAGCTT 3 cut(s) 725, 1988, 2414
HpaII CCGG 5 cut(s) 93, 198, 1500, 1739, 2817
HphI GGTGA 6 cut(s) 280, 632, 2600, 2727, 2851, 2887
Hpy166II GTNNAC 9 cut(s) 940, 1335, 1459, 1688, 2262, 2338, 2498, 2659, 2724
Hpy8I GTNNAC 9 cut(s) 940, 1335, 1459, 1688, 2262, 2338, 2498, 2659, 2724
HpyAV CCTTC 9 cut(s) 755, 865, 933, 1077, 1116, 1133, 1225, 1778, 2344
HpyCH4III ACNGT 6 cut(s) 1456, 1756, 2213, 2803, 2857, 2882
HpyCH4IV ACGT 5 cut(s) 597, 678, 1202, 1519, 2266
HpyF10VI GCNNNNNNNGC 6 cut(s) 207, 422, 562, 1149, 1721, 1730
HpySE526I ACGT 5 cut(s) 597, 678, 1202, 1519, 2266
Hsp92I GRCGYC 1 cut(s) 1519
Hsp92II CATG 9 cut(s) 105, 370, 1481, 2315, 2453, 2494, 2633, 2758, 2905
HspAI GCGC 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 2218
Kzo9I GATC 5 cut(s) 70, 515, 643, 2218, 2575
LguI GCTCTTC 2 cut(s) 850, 1896
LmnI GCTCC 5 cut(s) 110, 840, 1059, 1601, 2187
Lsp1109I GCAGC 6 cut(s) 100, 428, 967, 1477, 1733, 2081
LweI GCATC 2 cut(s) 468, 2081
MabI ACCWGGT 1 cut(s) 2139
MaeI CTAG 8 cut(s) 566, 672, 1052, 1776, 2292, 2654, 2664, 2923
MaeII ACGT 5 cut(s) 597, 678, 1202, 1519, 2266
MalI GATC 5 cut(s) 72, 517, 645, 2220, 2577
MbiI CCGCTC 2 cut(s) 1696, 1906
MboI GATC 5 cut(s) 70, 515, 643, 2218, 2575
MfeI CAATTG 3 cut(s) 2060, 2463, 2624
MhlI GDGCHC 3 cut(s) 845, 1245, 2726
MlyI GAGTC 4 cut(s) 116, 325, 992, 2654
MmeI TCCRAC 4 cut(s) 308, 1282, 1796, 1903
MroXI GAANNNNTTC 2 cut(s) 575, 2776
MseI TTAA 8 cut(s) 980, 1008, 1179, 2175, 2246, 2271, 2300, 2849
MslI CAYNNNNRTG 2 cut(s) 2343, 2900
MspA1I CMGCKG 1 cut(s) 958
MspI CCGG 5 cut(s) 93, 198, 1500, 1739, 2817
MspR9I CCNGG 5 cut(s) 1155, 1501, 1541, 2141, 2817
MunI CAATTG 3 cut(s) 2060, 2463, 2624
Mva1269I GAATGC 4 cut(s) 835, 1062, 1545, 2778
MvaI CCWGG 3 cut(s) 1155, 1541, 2141
MwoI GCNNNNNNNGC 6 cut(s) 207, 422, 562, 1149, 1721, 1730
NciI CCSGG 2 cut(s) 1501, 2817
NdeI CATATG 1 cut(s) 176
NdeII GATC 5 cut(s) 70, 515, 643, 2218, 2575
NlaIII CATG 9 cut(s) 105, 370, 1481, 2315, 2453, 2494, 2633, 2758, 2905
NlaIV GGNNCC 2 cut(s) 202, 2183
NmuCI GTSAC 6 cut(s) 131, 179, 1267, 2794, 2857, 2893
NspI RCATGY 1 cut(s) 1481
NspV TTCGAA 2 cut(s) 759, 2329
PagI TCATGA 1 cut(s) 2311
PciI ACATGT 1 cut(s) 1477
PciSI GCTCTTC 2 cut(s) 850, 1896
PctI GAATGC 4 cut(s) 835, 1062, 1545, 2778
PdmI GAANNNNTTC 2 cut(s) 575, 2776
PfeI GAWTC 7 cut(s) 61, 625, 914, 1158, 1373, 2157, 2318
PflMI CCANNNNNTGG 1 cut(s) 107
PkrI GCNGC 6 cut(s) 115, 418, 957, 1492, 1723, 2071
PleI GAGTC 4 cut(s) 116, 325, 992, 2654
PpsI GAGTC 4 cut(s) 116, 325, 992, 2654
PpuMI RGGWCCY 3 cut(s) 404, 764, 1190
PscI ACATGT 1 cut(s) 1477
PshAI GACNNNNGTC 1 cut(s) 2519
PshBI ATTAAT 1 cut(s) 1179
PsiI TTATAA 1 cut(s) 1167
Psp124BI GAGCTC 1 cut(s) 845
Psp1406I AACGTT 1 cut(s) 678
Psp5II RGGWCCY 3 cut(s) 404, 764, 1190
Psp6I CCWGG 3 cut(s) 1153, 1539, 2139
PspGI CCWGG 3 cut(s) 1153, 1539, 2139
PspN4I GGNNCC 2 cut(s) 202, 2183
PspPI GGNCC 9 cut(s) 66, 404, 764, 1151, 1190, 1250, 1709, 1925, 2009
PspPPI RGGWCCY 3 cut(s) 404, 764, 1190
PsrI GAACNNNNNNTAC 2 cut(s) 1821, 1853
PstI CTGCAG 2 cut(s) 418, 2071
PvuII CAGCTG 1 cut(s) 958
RsaI GTAC 8 cut(s) 320, 1043, 1321, 1702, 1830, 2153, 2337, 2446
RsaNI GTAC 8 cut(s) 319, 1042, 1320, 1701, 1829, 2152, 2336, 2445
RseI CAYNNNNRTG 2 cut(s) 2343, 2900
SacI GAGCTC 1 cut(s) 845
SapI GCTCTTC 2 cut(s) 850, 1896
SaqAI TTAA 8 cut(s) 980, 1008, 1179, 2175, 2246, 2271, 2300, 2849
SatI GCNGC 6 cut(s) 114, 417, 956, 1491, 1722, 2070
Sau3AI GATC 5 cut(s) 70, 515, 643, 2218, 2575
Sau96I GGNCC 9 cut(s) 66, 404, 764, 1151, 1190, 1250, 1709, 1925, 2009
ScaI AGTACT 1 cut(s) 1830
SchI GAGTC 4 cut(s) 116, 325, 992, 2654
ScrFI CCNGG 5 cut(s) 1155, 1501, 1541, 2141, 2817
SduI GDGCHC 3 cut(s) 845, 1245, 2726
SexAI ACCWGGT 1 cut(s) 2139
SfaNI GCATC 2 cut(s) 468, 2081
SfcI CTRYAG 4 cut(s) 414, 2017, 2067, 2853
SfuI TTCGAA 2 cut(s) 759, 2329
SinI GGWCC 7 cut(s) 404, 764, 1190, 1250, 1709, 1925, 2009
SmiMI CAYNNNNRTG 2 cut(s) 2343, 2900
SmlI CTYRAG 4 cut(s) 322, 736, 1436, 1463
SmoI CTYRAG 4 cut(s) 322, 736, 1436, 1463
SpeI ACTAGT 2 cut(s) 1775, 2653
SsiI CCGC 5 cut(s) 156, 618, 1696, 1906, 2456
SspI AATATT 2 cut(s) 907, 2362
SspMI CTAG 8 cut(s) 566, 672, 1052, 1776, 2292, 2654, 2664, 2923
SstI GAGCTC 1 cut(s) 845
StyD4I CCNGG 5 cut(s) 1153, 1499, 1539, 2139, 2815
StyI CCWWGG 2 cut(s) 1246, 2080
TaaI ACNGT 6 cut(s) 1456, 1756, 2213, 2803, 2857, 2882
TaiI ACGT 5 cut(s) 600, 681, 1205, 1522, 2269
TaqI TCGA 4 cut(s) 759, 827, 2329, 2610
TatI WGTACW 3 cut(s) 318, 1828, 2335
TfiI GAWTC 7 cut(s) 61, 625, 914, 1158, 1373, 2157, 2318
Tru1I TTAA 8 cut(s) 980, 1008, 1179, 2175, 2246, 2271, 2300, 2849
Tru9I TTAA 8 cut(s) 980, 1008, 1179, 2175, 2246, 2271, 2300, 2849
TscAI CASTG 4 cut(s) 48, 112, 1735, 2170
TseFI GTSAC 6 cut(s) 131, 179, 1267, 2794, 2857, 2893
TseI GCWGC 6 cut(s) 113, 416, 955, 1490, 1721, 2069
Tsp45I GTSAC 6 cut(s) 131, 179, 1267, 2794, 2857, 2893
TspDTI ATGAA 9 cut(s) 815, 884, 1248, 1398, 1735, 1992, 2300, 2328, 2646
TspGWI ACGGA 1 cut(s) 2169
TspRI CASTG 4 cut(s) 48, 112, 1735, 2170
Van91I CCANNNNNTGG 1 cut(s) 107
VneI GTGCAC 1 cut(s) 2722
VpaK11BI GGWCC 7 cut(s) 404, 764, 1190, 1250, 1709, 1925, 2009
VspI ATTAAT 1 cut(s) 1179
XapI RAATTY 8 cut(s) 37, 143, 379, 391, 571, 1505, 2569, 2675
XbaI TCTAGA 2 cut(s) 1051, 2663
XceI RCATGY 1 cut(s) 1481
XcmI CCANNNNNNNNNTGG 1 cut(s) 267
XmiI GTMKAC 3 cut(s) 939, 1334, 1687
XmnI GAANNNNTTC 2 cut(s) 575, 2776
XspI CTAG 8 cut(s) 566, 672, 1052, 1776, 2292, 2654, 2664, 2923
ZraI GACGTC 1 cut(s) 1520
ZrmI AGTACT 1 cut(s) 1830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.