RLG00000018789
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000088
Physical Location & Seq
Forward (+)
36198449 .. 36208011
9563 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018789

Sequence Viewer

Length: 2679 bp
ATGGTAGATGGGTTAGATGAGCTGAGAGAACGATGCTCTAGCGTAGCTTTTGCGCGAGCTCTGAGTTCAATCTTCCAGCAATGGCAGATTGAGGCCAAATGGAACACAACCAGAGATCCATGCAGTGGAGCGGCCATTGACGACTCCATCGAAATCAGCAACGAAGCATACAACCCTTTCGCCAAATGCAACTGTTCAAACTCCGCTTGCCACATTACCCAACTGTCAGATTACTGTCTCGTTTTGCACTTGAATTACGTCACTCCCACGCAATTTCGGTTCATCACACCATGCATTTGCAGGAAAGTTTCTACACTGGAGGTTGTTGGCGTAATTCCGGAACAGCTATGGACTCTGAAGTTTCTCACTCATCTCGATTTAGGTCACAATCACTTGACAGGACCTCTCTCTGCATCAATTGGAAATCTGAGCAGCTTGCAATATTTGAGCTTAGGCCATAACATGTTATCAGGCCAGCTACCAAAGGAACTCGGAAATCTCTCTAATTTGACATTCTTGGCTATTGGGACTATGAACTTTTCCGGAGGTCTACCACATGAGCTAGGGAATTTATTAAATTTACAACAGATTTACTTGGATAGTTCTGGAGTTAGCGGTGAGATTCCACCGACGTTTGCTAATCTACGAAACTTGACCATATTCCGGGTGTCAGACACAGAACTCTCTGGCAGGATACCTGACTTCGTAGGATATTGGTCAAAGCTTACTTCCTTGGTATTTCAAGGGAACTCTTTTGAAGGTCCTATACCAGCTACACTGTCCAATCTGACTTCTCTTGAGGAGTTGAGAATTAGTGATATAATATCTACTGCCAGTGGGAACTCTTCTCTTGGATTTATTAAGGATATGAAGTCAAGATCTCTAAAAGTCCTAGTGCTGCGAAATAACAATATTTCTGATTCAATTCCTTCTAATATTGGAGAGTTGCAAAGTTTGTATCACCTCAAACCCATGTACCGAACCTGGAACCTTTTATTGGGTTGGTTGAGAGAGCAGAGATACGCAGTCAGGTTGAAATGCCTCCAAAGGAACTTTCCTTGCAATGTTAACCCTGGAATCCATCGTTACATTGGGATTAAATGTGGTGGTCCTCGGATCACGTCTTCCAATGGGATTGTGTATGATCAGGAAAATGAGACCCTTGGTCCAGCTACATATTTTGTGACTGGCACAAGCAGATGGGGAGTTAGCAATGTTGGTATTAAAACAGAGTATCAGGATGGATATATCACGGGAAACATTCCTGAGTATACAGTTTCCTCGTCATCTCAATTCACGAATACTTCAGATCCTGAGCTATTCCAGACTGCAAGGATCTCTGCTTCATCACTTAGATACTATGGCTTGGGGCTGGAGAATGGTAACTACACGGTCAAACTTCAGTTTGCAGAACAAAAAATCCCAGATACTCCAAAAAATCTTGGGAGACGTGTATTTGATATATATATCCAGAAGGAATATAAGGCCCAGGTTTCACAGAACTACCTTGAAATTCATCTCTTTTGGGCTGGAAAGGGGACGTGCTGCGTACCTGAGGTTGGTACTTATGGACCTGTTATTTCAGCCATCAGTGCTACACCAGGTAATAACAATATAACACGCAAATTTCAATATTTTCTAGTATTATTATTTGCCAACATAAAGTTTCATTGTATCTTTGCAGGTACTCTTAATGATGGAAGAGTAATTGCCGTGAAGCAATTGTCTGCAGCATCCCAGCAAGGAAAAAATGAGTTTGTAGCTGAAATTGCCACCATATCTGCCGTGCGACACAATAACCTAGTGAATTTGCATGGATTCTGCACTGAGGGAGTTAAACGGCTCCTTGTCTATGAGTATCTGGAAAACAAGAGTCTTGATCATGCATTATTTGGAAAAAGAAGTTTGAATCTTGATTGGTCATCAAGATTTGATATATGCTTGGGCGTGGCTAAAGGTTTGACTTATCTCCATGAGGAATCAAGGGTTCGGATTGTACACAGAGATGTGAAGGCCAGTAATATTCTGCTTGACTCTAATCTCATCCCCAAAATATCAGATTTCGGTTTGGCCAAGCTTTATGATGATAAAAAGACCCACATCAGTACTCGTGTTGCGGGAACAATAGGGTATCTTGCACCGGAATATGCCATGCGTGGACACTTGACACAGAAGACTGATGTGTTTGCCTTTGGTGTTCTTGCTCTAGAAATTGTCAGTGGTAGGCCAAATTCTGACCGGAGCTTGGATGGCGAGATGATTTATCTTCTCGAATGGGCTTGGCACTTGCATGAAAACAAACGCGAAGTTGAACTAGTGGACTCTAGGTTATCAGAATTCAATGAGGAAGAGGCAAGACGAATAACCAAGATAGGACTTTTGTGCACTCAAACATCACCAATGCAACGACCACCGATGTCGCGTGTGGTGGGAATGCTTTCAGGAGATATTGAAGTAATGACAGTAACTTCAAAGCCTAGTTACTTGACAGACTGGGAATTTGACGATGCAAGTACTTTCACCAGTCTAACCACTGATATGTCAGCAAAAGGAACTGATCCGAGCTTTTACAACTCACCGGCGAGTGCAAGCACTTTGGGGGATCCAGGGCAGCTTTCTAAAAATGCCACGGATGCGTTGCTTCATAATGTTGTTGATACCCAATCCCTACCGACTTAA

Protein Analysis

893

Amino Acids

99.11

Weight (kDa)

6.51

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 112 - 199 5.9e-08 Leucine-rich repeat region
LRR_14 PF23598 187 - 339 5.3e-10 Leucine-rich repeat region
Malectin PF11721 364 - 493 1.6e-23 Malectin domain
PK_Tyr_Ser-Thr PF07714 558 - 811 8.1e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 564 - 746 1.7e-37 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1673
AccB7I CCANNNNNTGG 1 cut(s) 125
AccBSI CCGCTC 1 cut(s) 131
AccI GTMKAC 2 cut(s) 550, 1271
AccII CGCG 3 cut(s) 55, 2304, 2422
AccIII TCCGGA 2 cut(s) 337, 542
AciI CCGC 4 cut(s) 131, 204, 615, 2117
AclWI GGATC 7 cut(s) 110, 1124, 1304, 1343, 2552, 2597, 2610
AcoI YGGCCR 2 cut(s) 132, 2070
AcsI RAATTY 8 cut(s) 568, 577, 1512, 1625, 1807, 2230, 2336, 2498
AcuI CTGAAG 3 cut(s) 377, 1290, 1385
AfaI GTAC 7 cut(s) 977, 1551, 1564, 1687, 1998, 2107, 2515
AfiI CCNNNNNNNGG 5 cut(s) 125, 663, 997, 1559, 2245
AflIII ACRYGT 2 cut(s) 462, 1450
AhdI GACNNNNNGTC 1 cut(s) 1164
AhlI ACTAGT 1 cut(s) 2314
AjiI CACGTC 3 cut(s) 1122, 1451, 1542
AjnI CCWGG 5 cut(s) 983, 1072, 1488, 1600, 2605
AjuI GAANNNNNNNTTGG 4 cut(s) 980, 1012, 1038, 1070
AloI GAACNNNNNNTCC 2 cut(s) 1404, 1436
Alw21I GWGCWC 2 cut(s) 61, 2387
Alw26I GTCTC 3 cut(s) 242, 1151, 1441
Alw44I GTGCAC 1 cut(s) 2383
AlwI GGATC 7 cut(s) 110, 1124, 1304, 1343, 2552, 2597, 2610
AlwNI CAGNNNCTG 1 cut(s) 1313
Aor13HI TCCGGA 2 cut(s) 337, 542
AoxI GGCC 8 cut(s) 93, 132, 454, 472, 1485, 2013, 2070, 2225
ApaLI GTGCAC 1 cut(s) 2383
ApeKI GCWGC 5 cut(s) 432, 898, 1545, 1730, 2611
ApoI RAATTY 8 cut(s) 568, 577, 1512, 1625, 1807, 2230, 2336, 2498
ArsI GACNNNNNNTTYG 2 cut(s) 2483, 2515
Asp700I GAANNNNTTC 1 cut(s) 2437
AspLEI GCGC 1 cut(s) 55
AspS9I GGNCC 6 cut(s) 401, 761, 1109, 1166, 1486, 1571
AsuC2I CCSGG 1 cut(s) 665
AsuHPI GGTGA 5 cut(s) 629, 953, 2388, 2512, 2568
AvaII GGWCC 5 cut(s) 401, 761, 1109, 1166, 1571
AxyI CCTNAGG 1 cut(s) 1554
BaeGI GKGCMC 1 cut(s) 2387
BaeI ACNNNNGTAYC 2 cut(s) 959, 992
BalI TGGCCA 1 cut(s) 2072
BamHI GGATCC 1 cut(s) 2602
BanII GRGCYC 1 cut(s) 61
BarI GAAGNNNNNNTAC 2 cut(s) 750, 782
BauI CACGAG 1 cut(s) 2109
BbsI GAAGAC 2 cut(s) 1116, 2180
Bbv12I GWGCWC 2 cut(s) 61, 2387
BbvI GCAGC 5 cut(s) 444, 885, 1532, 1742, 2623
BccI CCATC 8 cut(s) 2, 155, 1089, 1194, 1235, 1595, 1691, 2243
BceAI ACGGC 3 cut(s) 1697, 1769, 1856
BciT130I CCWGG 5 cut(s) 985, 1074, 1490, 1602, 2607
BciVI GTATCC 1 cut(s) 687
BclI TGATCA 2 cut(s) 1144, 1879
BcnI CCSGG 1 cut(s) 665
BcoDI GTCTC 3 cut(s) 242, 1151, 1441
BcuI ACTAGT 1 cut(s) 2314
BfaI CTAG 9 cut(s) 39, 563, 893, 1640, 1802, 2207, 2315, 2325, 2478
BfmI CTRYAG 1 cut(s) 1728
BfuAI ACCTGC 1 cut(s) 1673
BfuI GTATCC 1 cut(s) 687
BglII AGATCT 1 cut(s) 878
BisI GCNGC 6 cut(s) 132, 433, 899, 1546, 1731, 2612
BlsI GCNGC 6 cut(s) 133, 434, 900, 1547, 1732, 2613
BmcAI AGTACT 2 cut(s) 2107, 2515
Bme1390I CCNGG 6 cut(s) 665, 985, 1074, 1490, 1602, 2607
Bme18I GGWCC 5 cut(s) 401, 761, 1109, 1166, 1571
BmeRI GACNNNNNGTC 1 cut(s) 1164
BmgBI CACGTC 3 cut(s) 1122, 1451, 1542
BmgT120I GGNCC 6 cut(s) 401, 761, 1109, 1166, 1486, 1571
BmiI GGNNCC 3 cut(s) 989, 1844, 2604
BmrFI CCNGG 6 cut(s) 665, 985, 1074, 1490, 1602, 2607
BmrI ACTGGG 1 cut(s) 2503
BmsI GCATC 5 cut(s) 23, 422, 1742, 2497, 2623
BmuI ACTGGG 1 cut(s) 2503
BpiI GAAGAC 2 cut(s) 1116, 2180
BpmI CTGGAG 3 cut(s) 338, 627, 1394
Bpu10I CCTNAGC 2 cut(s) 451, 1314
BpuEI CTTGAG 1 cut(s) 818
BpuMI CCSGG 1 cut(s) 665
BsaI GGTCTC 1 cut(s) 1151
BsaJI CCNNGG 7 cut(s) 732, 1072, 1112, 1162, 1488, 2606, 2628
BsaWI WCCGGW 4 cut(s) 337, 542, 2140, 2238
Bsc4I CCNNNNNNNGG 5 cut(s) 125, 663, 997, 1559, 2245
Bse118I RCCGGY 1 cut(s) 2578
Bse1I ACTGG 6 cut(s) 321, 834, 1192, 2016, 2498, 2523
Bse21I CCTNAGG 1 cut(s) 1554
Bse3DI GCAATG 3 cut(s) 86, 1069, 1219
BseAI TCCGGA 2 cut(s) 337, 542
BseBI CCWGG 5 cut(s) 985, 1074, 1490, 1602, 2607
BseDI CCNNGG 7 cut(s) 732, 1072, 1112, 1162, 1488, 2606, 2628
BseGI GGATG 5 cut(s) 1246, 1733, 2043, 2254, 2638
BseLI CCNNNNNNNGG 5 cut(s) 125, 663, 997, 1559, 2245
BseMI GCAATG 3 cut(s) 86, 1069, 1219
BseMII CTCAG 7 cut(s) 14, 53, 419, 1257, 1305, 1545, 1818
BseNI ACTGG 6 cut(s) 321, 834, 1192, 2016, 2498, 2523
BseRI GAGGAG 1 cut(s) 815
BseSI GKGCMC 1 cut(s) 2387
BseXI GCAGC 5 cut(s) 444, 885, 1532, 1742, 2623
BseYI CCCAGC 1 cut(s) 1737
BsgI GTGCAG 1 cut(s) 1807
Bsh1236I CGCG 3 cut(s) 55, 2304, 2422
BshFI GGCC 8 cut(s) 95, 134, 456, 474, 1487, 2015, 2072, 2227
BsiHKAI GWGCWC 2 cut(s) 61, 2387
BsiSI CCGG 6 cut(s) 338, 543, 664, 2141, 2239, 2579
BslFI GGGAC 2 cut(s) 541, 1552
BslI CCNNNNNNNGG 5 cut(s) 125, 663, 997, 1559, 2245
BsmAI GTCTC 3 cut(s) 242, 1151, 1441
BsmBI CGTCTC 1 cut(s) 1441
BsmFI GGGAC 2 cut(s) 541, 1552
BsmI GAATGC 1 cut(s) 2439
BsnI GGCC 8 cut(s) 95, 134, 456, 474, 1487, 2015, 2072, 2227
Bso31I GGTCTC 1 cut(s) 1151
Bsp1286I GDGCHC 2 cut(s) 61, 2387
Bsp13I TCCGGA 2 cut(s) 337, 542
Bsp1407I TGTACA 1 cut(s) 1996
Bsp143I GATC 9 cut(s) 115, 878, 1116, 1144, 1309, 1335, 1879, 2557, 2602
BspACI CCGC 4 cut(s) 131, 204, 615, 2117
BspANI GGCC 8 cut(s) 95, 134, 456, 474, 1487, 2015, 2072, 2227
BspCNI CTCAG 7 cut(s) 15, 54, 420, 1258, 1306, 1546, 1819
BspEI TCCGGA 2 cut(s) 337, 542
BspFNI CGCG 3 cut(s) 55, 2304, 2422
BspLI GGNNCC 3 cut(s) 989, 1844, 2604
BspMAI CTGCAG 1 cut(s) 1732
BspMI ACCTGC 1 cut(s) 1673
BspPI GGATC 7 cut(s) 110, 1124, 1304, 1343, 2552, 2597, 2610
BspTNI GGTCTC 1 cut(s) 1151
BsrBI CCGCTC 1 cut(s) 131
BsrDI GCAATG 3 cut(s) 86, 1069, 1219
BsrFI RCCGGY 1 cut(s) 2578
BsrGI TGTACA 1 cut(s) 1996
BsrI ACTGG 6 cut(s) 321, 834, 1192, 2016, 2498, 2523
BssAI RCCGGY 1 cut(s) 2578
BssECI CCNNGG 7 cut(s) 732, 1072, 1112, 1162, 1488, 2606, 2628
BssMI GATC 9 cut(s) 115, 878, 1116, 1144, 1309, 1335, 1879, 2557, 2602
BssNAI GTATAC 1 cut(s) 1272
BssSI CACGAG 1 cut(s) 2109
BssT1I CCWWGG 2 cut(s) 732, 1162
Bst1107I GTATAC 1 cut(s) 1272
Bst2BI CACGAG 1 cut(s) 2109
Bst2UI CCWGG 5 cut(s) 985, 1074, 1490, 1602, 2607
Bst4CI ACNGT 7 cut(s) 194, 225, 236, 780, 1276, 1393, 2464
Bst6I CTCTTC 3 cut(s) 850, 1696, 2343
BstAUI TGTACA 1 cut(s) 1996
BstC8I GCNNGC 5 cut(s) 57, 208, 437, 476, 2590
BstDEI CTNAG 9 cut(s) 23, 62, 428, 451, 1266, 1314, 1352, 1554, 1827
BstDSI CCRYGG 1 cut(s) 2628
BstF5I GGATG 5 cut(s) 1246, 1733, 2043, 2254, 2638
BstFNI CGCG 3 cut(s) 55, 2304, 2422
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 9 cut(s) 118, 881, 1119, 1147, 1312, 1338, 1882, 2560, 2605
BstMAI GTCTC 3 cut(s) 242, 1151, 1441
BstMBI GATC 9 cut(s) 115, 878, 1116, 1144, 1309, 1335, 1879, 2557, 2602
BstMWI GCNNNNNNNGC 4 cut(s) 1592, 1769, 2250, 2633
BstNI CCWGG 5 cut(s) 985, 1074, 1490, 1602, 2607
BstNSI RCATGY 1 cut(s) 466
BstSCI CCNGG 6 cut(s) 663, 983, 1072, 1488, 1600, 2605
BstSFI CTRYAG 1 cut(s) 1728
BstSLI GKGCMC 1 cut(s) 2387
BstUI CGCG 3 cut(s) 55, 2304, 2422
BstV1I GCAGC 5 cut(s) 444, 885, 1532, 1742, 2623
BstV2I GAAGAC 2 cut(s) 1116, 2180
BstX2I RGATCY 5 cut(s) 115, 878, 1309, 1335, 2602
BstYI RGATCY 5 cut(s) 115, 878, 1309, 1335, 2602
BstZ17I GTATAC 1 cut(s) 1272
Bsu36I CCTNAGG 1 cut(s) 1554
BsuI GTATCC 1 cut(s) 687
BsuRI GGCC 8 cut(s) 95, 134, 456, 474, 1487, 2015, 2072, 2227
BtgI CCRYGG 1 cut(s) 2628
BtrI CACGTC 3 cut(s) 1122, 1451, 1542
BtsCI GGATG 5 cut(s) 1246, 1733, 2043, 2254, 2638
BtsI GCAGTG 1 cut(s) 130
BtsIMutI CAGTG 8 cut(s) 130, 314, 776, 841, 1597, 1824, 2224, 2532
BveI ACCTGC 1 cut(s) 1673
Cac8I GCNNGC 5 cut(s) 57, 208, 437, 476, 2590
CaiI CAGNNNCTG 1 cut(s) 1313
CfoI GCGC 1 cut(s) 55
Cfr10I RCCGGY 1 cut(s) 2578
Cfr13I GGNCC 6 cut(s) 401, 761, 1109, 1166, 1486, 1571
CsiI ACCWGGT 1 cut(s) 1600
Csp6I GTAC 7 cut(s) 976, 1550, 1563, 1686, 1997, 2106, 2514
CviQI GTAC 7 cut(s) 976, 1550, 1563, 1686, 1997, 2106, 2514
DdeI CTNAG 9 cut(s) 23, 62, 428, 451, 1266, 1314, 1352, 1554, 1827
DpnI GATC 9 cut(s) 117, 880, 1118, 1146, 1311, 1337, 1881, 2559, 2604
DpnII GATC 9 cut(s) 115, 878, 1116, 1144, 1309, 1335, 1879, 2557, 2602
DriI GACNNNNNGTC 1 cut(s) 1164
EaeI YGGCCR 2 cut(s) 132, 2070
Eam1104I CTCTTC 3 cut(s) 850, 1696, 2343
Eam1105I GACNNNNNGTC 1 cut(s) 1164
EarI CTCTTC 3 cut(s) 850, 1696, 2343
Ecl136II GAGCTC 1 cut(s) 59
Eco130I CCWWGG 2 cut(s) 732, 1162
Eco24I GRGCYC 1 cut(s) 61
Eco31I GGTCTC 1 cut(s) 1151
Eco47I GGWCC 5 cut(s) 401, 761, 1109, 1166, 1571
Eco53kI GAGCTC 1 cut(s) 59
Eco57I CTGAAG 3 cut(s) 377, 1290, 1385
Eco81I CCTNAGG 1 cut(s) 1554
EcoICRI GAGCTC 1 cut(s) 59
EcoO109I RGGNCCY 2 cut(s) 401, 761
EcoRI GAATTC 1 cut(s) 2336
EcoRII CCWGG 5 cut(s) 983, 1072, 1488, 1600, 2605
EcoT14I CCWWGG 2 cut(s) 732, 1162
EcoT22I ATGCAT 2 cut(s) 296, 1888
EcoT38I GRGCYC 1 cut(s) 61
ErhI CCWWGG 2 cut(s) 732, 1162
Esp3I CGTCTC 1 cut(s) 1441
FaqI GGGAC 2 cut(s) 541, 1552
FauI CCCGC 1 cut(s) 2110
FbaI TGATCA 2 cut(s) 1144, 1879
FblI GTMKAC 2 cut(s) 550, 1271
Fnu4HI GCNGC 6 cut(s) 132, 433, 899, 1546, 1731, 2612
FokI GGATG 5 cut(s) 1253, 1720, 2030, 2261, 2645
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 6 cut(s) 132, 433, 899, 1546, 1731, 2612
FspBI CTAG 9 cut(s) 39, 563, 893, 1640, 1802, 2207, 2315, 2325, 2478
GlaI GCGC 1 cut(s) 54
GluI GCNGC 6 cut(s) 132, 433, 899, 1546, 1731, 2612
GsaI CCCAGC 1 cut(s) 1741
GsuI CTGGAG 3 cut(s) 338, 627, 1394
HaeIII GGCC 8 cut(s) 95, 134, 456, 474, 1487, 2015, 2072, 2227
HapII CCGG 6 cut(s) 338, 543, 664, 2141, 2239, 2579
HhaI GCGC 1 cut(s) 55
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HincII GTYRAC 1 cut(s) 1069
HindII GTYRAC 1 cut(s) 1069
HindIII AAGCTT 2 cut(s) 722, 2075
HpaI GTTAAC 1 cut(s) 1069
HpaII CCGG 6 cut(s) 338, 543, 664, 2141, 2239, 2579
HphI GGTGA 5 cut(s) 629, 953, 2388, 2512, 2568
Hpy166II GTNNAC 7 cut(s) 551, 1069, 1272, 1999, 2159, 2320, 2385
Hpy8I GTNNAC 7 cut(s) 551, 1069, 1272, 1999, 2159, 2320, 2385
Hpy99I CGWCG 1 cut(s) 634
HpyAV CCTTC 4 cut(s) 752, 939, 1468, 2005
HpyCH4III ACNGT 7 cut(s) 194, 225, 236, 780, 1276, 1393, 2464
HpyCH4IV ACGT 5 cut(s) 258, 632, 1121, 1450, 1541
HpyF10VI GCNNNNNNNGC 4 cut(s) 1592, 1769, 2250, 2633
HpyF3I CTNAG 9 cut(s) 23, 62, 428, 451, 1266, 1314, 1352, 1554, 1827
HpySE526I ACGT 5 cut(s) 258, 632, 1121, 1450, 1541
HspAI GCGC 1 cut(s) 53
Kpn2I TCCGGA 2 cut(s) 337, 542
Ksp22I TGATCA 2 cut(s) 1144, 1879
KspAI GTTAAC 1 cut(s) 1069
Kzo9I GATC 9 cut(s) 115, 878, 1116, 1144, 1309, 1335, 1879, 2557, 2602
LmnI GCTCC 3 cut(s) 128, 1848, 2241
Lsp1109I GCAGC 5 cut(s) 444, 885, 1532, 1742, 2623
LweI GCATC 5 cut(s) 23, 422, 1742, 2497, 2623
MabI ACCWGGT 1 cut(s) 1600
MaeI CTAG 9 cut(s) 39, 563, 893, 1640, 1802, 2207, 2315, 2325, 2478
MaeII ACGT 5 cut(s) 258, 632, 1121, 1450, 1541
MaeIII GTNAC 7 cut(s) 259, 383, 1085, 1183, 1382, 2464, 2480
MalI GATC 9 cut(s) 117, 880, 1118, 1146, 1311, 1337, 1881, 2559, 2604
MbiI CCGCTC 1 cut(s) 131
MboI GATC 9 cut(s) 115, 878, 1116, 1144, 1309, 1335, 1879, 2557, 2602
MboII GAAGA 7 cut(s) 64, 837, 1116, 1713, 2185, 2258, 2360
MfeI CAATTG 2 cut(s) 417, 1721
MflI RGATCY 5 cut(s) 115, 878, 1309, 1335, 2602
MhlI GDGCHC 2 cut(s) 61, 2387
MlsI TGGCCA 1 cut(s) 2072
MluNI TGGCCA 1 cut(s) 2072
MlyI GAGTC 5 cut(s) 137, 346, 1882, 2027, 2315
Mox20I TGGCCA 1 cut(s) 2072
Mph1103I ATGCAT 2 cut(s) 296, 1888
MroI TCCGGA 2 cut(s) 337, 542
MroXI GAANNNNTTC 1 cut(s) 2437
MscI TGGCCA 1 cut(s) 2072
MseI TTAA 8 cut(s) 575, 861, 1068, 1098, 1224, 1692, 1836, 2677
MslI CAYNNNNRTG 3 cut(s) 2004, 2289, 2537
Msp20I TGGCCA 1 cut(s) 2072
MspI CCGG 6 cut(s) 338, 543, 664, 2141, 2239, 2579
MspR9I CCNGG 6 cut(s) 665, 985, 1074, 1490, 1602, 2607
MunI CAATTG 2 cut(s) 417, 1721
Mva1269I GAATGC 1 cut(s) 2439
MvaI CCWGG 5 cut(s) 985, 1074, 1490, 1602, 2607
MvnI CGCG 3 cut(s) 55, 2304, 2422
MwoI GCNNNNNNNGC 4 cut(s) 1592, 1769, 2250, 2633
NciI CCSGG 1 cut(s) 665
NdeII GATC 9 cut(s) 115, 878, 1116, 1144, 1309, 1335, 1879, 2557, 2602
NlaIV GGNNCC 3 cut(s) 989, 1844, 2604
NmuCI GTSAC 3 cut(s) 259, 383, 1183
NsiI ATGCAT 2 cut(s) 296, 1888
NspI RCATGY 1 cut(s) 466
PciI ACATGT 1 cut(s) 462
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PctI GAATGC 1 cut(s) 2439
PdmI GAANNNNTTC 1 cut(s) 2437
PfeI GAWTC 6 cut(s) 622, 920, 1077, 1818, 1909, 1979
PflMI CCANNNNNTGG 1 cut(s) 125
PkrI GCNGC 6 cut(s) 133, 434, 900, 1547, 1732, 2613
PleI GAGTC 5 cut(s) 137, 346, 1881, 2027, 2315
PpsI GAGTC 5 cut(s) 137, 346, 1881, 2027, 2315
PpuMI RGGWCCY 2 cut(s) 401, 761
PscI ACATGT 1 cut(s) 462
Psp124BI GAGCTC 1 cut(s) 61
Psp5II RGGWCCY 2 cut(s) 401, 761
Psp6I CCWGG 5 cut(s) 983, 1072, 1488, 1600, 2605
PspFI CCCAGC 1 cut(s) 1737
PspGI CCWGG 5 cut(s) 983, 1072, 1488, 1600, 2605
PspN4I GGNNCC 3 cut(s) 989, 1844, 2604
PspPI GGNCC 6 cut(s) 401, 761, 1109, 1166, 1486, 1571
PspPPI RGGWCCY 2 cut(s) 401, 761
PstI CTGCAG 1 cut(s) 1732
PstNI CAGNNNCTG 1 cut(s) 1313
PsuI RGATCY 5 cut(s) 115, 878, 1309, 1335, 2602
RsaI GTAC 7 cut(s) 977, 1551, 1564, 1687, 1998, 2107, 2515
RsaNI GTAC 7 cut(s) 976, 1550, 1563, 1686, 1997, 2106, 2514
RseI CAYNNNNRTG 3 cut(s) 2004, 2289, 2537
SacI GAGCTC 1 cut(s) 61
SaqAI TTAA 8 cut(s) 575, 861, 1068, 1098, 1224, 1692, 1836, 2677
SatI GCNGC 6 cut(s) 132, 433, 899, 1546, 1731, 2612
Sau3AI GATC 9 cut(s) 115, 878, 1116, 1144, 1309, 1335, 1879, 2557, 2602
Sau96I GGNCC 6 cut(s) 401, 761, 1109, 1166, 1486, 1571
ScaI AGTACT 2 cut(s) 2107, 2515
SchI GAGTC 5 cut(s) 137, 346, 1882, 2027, 2315
ScrFI CCNGG 6 cut(s) 665, 985, 1074, 1490, 1602, 2607
SduI GDGCHC 2 cut(s) 61, 2387
SexAI ACCWGGT 1 cut(s) 1600
SfaNI GCATC 5 cut(s) 23, 422, 1742, 2497, 2623
SfcI CTRYAG 1 cut(s) 1728
SgrAI CRCCGGYG 1 cut(s) 2578
SinI GGWCC 5 cut(s) 401, 761, 1109, 1166, 1571
SmiMI CAYNNNNRTG 3 cut(s) 2004, 2289, 2537
SmlI CTYRAG 1 cut(s) 797
SmoI CTYRAG 1 cut(s) 797
SpeI ACTAGT 1 cut(s) 2314
SsiI CCGC 4 cut(s) 131, 204, 615, 2117
SspI AATATT 5 cut(s) 443, 913, 937, 1634, 2023
SspMI CTAG 9 cut(s) 39, 563, 893, 1640, 1802, 2207, 2315, 2325, 2478
SstI GAGCTC 1 cut(s) 61
StyD4I CCNGG 6 cut(s) 663, 983, 1072, 1488, 1600, 2605
StyI CCWWGG 2 cut(s) 732, 1162
TaaI ACNGT 7 cut(s) 194, 225, 236, 780, 1276, 1393, 2464
TaiI ACGT 5 cut(s) 261, 635, 1124, 1453, 1544
TaqI TCGA 3 cut(s) 150, 375, 2271
TatI WGTACW 3 cut(s) 1996, 2105, 2513
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 6 cut(s) 622, 920, 1077, 1818, 1909, 1979
Tru1I TTAA 8 cut(s) 575, 861, 1068, 1098, 1224, 1692, 1836, 2677
Tru9I TTAA 8 cut(s) 575, 861, 1068, 1098, 1224, 1692, 1836, 2677
TscAI CASTG 8 cut(s) 130, 321, 783, 841, 1597, 1831, 2224, 2539
TseFI GTSAC 3 cut(s) 259, 383, 1183
TseI GCWGC 5 cut(s) 432, 898, 1545, 1730, 2611
Tsp45I GTSAC 3 cut(s) 259, 383, 1183
TspDTI ATGAA 8 cut(s) 271, 548, 884, 1335, 1505, 1658, 2307, 2633
TspGWI ACGGA 1 cut(s) 2645
TspRI CASTG 8 cut(s) 130, 321, 783, 841, 1597, 1831, 2224, 2539
Van91I CCANNNNNTGG 1 cut(s) 125
VneI GTGCAC 1 cut(s) 2383
VpaK11BI GGWCC 5 cut(s) 401, 761, 1109, 1166, 1571
XapI RAATTY 8 cut(s) 568, 577, 1512, 1625, 1807, 2230, 2336, 2498
XbaI TCTAGA 1 cut(s) 2206
XceI RCATGY 1 cut(s) 466
XcmI CCANNNNNNNNNTGG 1 cut(s) 1088
XmiI GTMKAC 2 cut(s) 550, 1271
XmnI GAANNNNTTC 1 cut(s) 2437
XspI CTAG 9 cut(s) 39, 563, 893, 1640, 1802, 2207, 2315, 2325, 2478
ZrmI AGTACT 2 cut(s) 2107, 2515
Zsp2I ATGCAT 2 cut(s) 296, 1888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.