Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 266 |
| AccI | GTMKAC | 1 cut(s) | 199 |
| AccII | CGCG | 1 cut(s) | 594 |
| AciI | CCGC | 1 cut(s) | 357 |
| AclWI | GGATC | 1 cut(s) | 368 |
| AcsI | RAATTY | 2 cut(s) | 367, 671 |
| AcuI | CTGAAG | 2 cut(s) | 436, 718 |
| AfaI | GTAC | 5 cut(s) | 128, 214, 629, 688, 760 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 209, 293 |
| AgsI | TTSAA | 2 cut(s) | 623, 795 |
| AjnI | CCWGG | 1 cut(s) | 173 |
| AluBI | AGCT | 2 cut(s) | 80, 738 |
| AluI | AGCT | 2 cut(s) | 80, 738 |
| Alw21I | GWGCWC | 1 cut(s) | 557 |
| Alw26I | GTCTC | 2 cut(s) | 47, 594 |
| Alw44I | GTGCAC | 1 cut(s) | 553 |
| AlwI | GGATC | 1 cut(s) | 368 |
| AoxI | GGCC | 1 cut(s) | 362 |
| ApaLI | GTGCAC | 1 cut(s) | 553 |
| ApoI | RAATTY | 2 cut(s) | 367, 671 |
| AspLEI | GCGC | 2 cut(s) | 267, 286 |
| AspS9I | GGNCC | 3 cut(s) | 17, 74, 221 |
| AsuHPI | GGTGA | 3 cut(s) | 558, 649, 689 |
| AvaII | GGWCC | 3 cut(s) | 17, 74, 221 |
| BaeGI | GKGCMC | 2 cut(s) | 69, 557 |
| BbsI | GAAGAC | 1 cut(s) | 317 |
| Bbv12I | GWGCWC | 1 cut(s) | 557 |
| BccI | CCATC | 4 cut(s) | 102, 245, 662, 674 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 305, 339 |
| BciT130I | CCWGG | 1 cut(s) | 175 |
| BcoDI | GTCTC | 2 cut(s) | 47, 594 |
| BfaI | CTAG | 3 cut(s) | 344, 701, 720 |
| BfmI | CTRYAG | 1 cut(s) | 10 |
| BmcAI | AGTACT | 1 cut(s) | 688 |
| Bme1390I | CCNGG | 1 cut(s) | 175 |
| Bme18I | GGWCC | 3 cut(s) | 17, 74, 221 |
| BmgT120I | GGNCC | 3 cut(s) | 17, 74, 221 |
| BmrFI | CCNGG | 1 cut(s) | 175 |
| BmsI | GCATC | 2 cut(s) | 634, 766 |
| BpiI | GAAGAC | 1 cut(s) | 317 |
| BpmI | CTGGAG | 1 cut(s) | 652 |
| Bpu10I | CCTNAGC | 1 cut(s) | 135 |
| BsaJI | CCNNGG | 3 cut(s) | 20, 70, 174 |
| BsaWI | WCCGGW | 1 cut(s) | 269 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 209, 293 |
| Bse1I | ACTGG | 2 cut(s) | 635, 694 |
| Bse3DI | GCAATG | 1 cut(s) | 127 |
| BseBI | CCWGG | 1 cut(s) | 175 |
| BseDI | CCNNGG | 3 cut(s) | 20, 70, 174 |
| BseGI | GGATG | 1 cut(s) | 781 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 209, 293 |
| BseMI | GCAATG | 1 cut(s) | 127 |
| BseMII | CTCAG | 2 cut(s) | 149, 572 |
| BseNI | ACTGG | 2 cut(s) | 635, 694 |
| BseRI | GAGGAG | 1 cut(s) | 221 |
| BseSI | GKGCMC | 2 cut(s) | 69, 557 |
| Bsh1236I | CGCG | 1 cut(s) | 594 |
| BshFI | GGCC | 1 cut(s) | 364 |
| BsiHKAI | GWGCWC | 1 cut(s) | 557 |
| BsiSI | CCGG | 1 cut(s) | 270 |
| BslFI | GGGAC | 1 cut(s) | 202 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 209, 293 |
| BsmAI | GTCTC | 2 cut(s) | 47, 594 |
| BsmBI | CGTCTC | 1 cut(s) | 47 |
| BsmFI | GGGAC | 1 cut(s) | 202 |
| BsmI | GAATGC | 1 cut(s) | 609 |
| BsnI | GGCC | 1 cut(s) | 364 |
| Bsp1286I | GDGCHC | 2 cut(s) | 69, 557 |
| Bsp1407I | TGTACA | 1 cut(s) | 126 |
| Bsp143I | GATC | 2 cut(s) | 373, 597 |
| BspACI | CCGC | 1 cut(s) | 357 |
| BspANI | GGCC | 1 cut(s) | 364 |
| BspCNI | CTCAG | 2 cut(s) | 148, 571 |
| BspFNI | CGCG | 1 cut(s) | 594 |
| BspMAI | CTGCAG | 1 cut(s) | 14 |
| BspPI | GGATC | 1 cut(s) | 368 |
| BsrDI | GCAATG | 1 cut(s) | 127 |
| BsrGI | TGTACA | 1 cut(s) | 126 |
| BsrI | ACTGG | 2 cut(s) | 635, 694 |
| BssECI | CCNNGG | 3 cut(s) | 20, 70, 174 |
| BssMI | GATC | 2 cut(s) | 373, 597 |
| BssNAI | GTATAC | 1 cut(s) | 200 |
| BssT1I | CCWWGG | 1 cut(s) | 70 |
| Bst1107I | GTATAC | 1 cut(s) | 200 |
| Bst2UI | CCWGG | 1 cut(s) | 175 |
| Bst4CI | ACNGT | 2 cut(s) | 64, 698 |
| Bst6I | CTCTTC | 1 cut(s) | 810 |
| BstAUI | TGTACA | 1 cut(s) | 126 |
| BstC8I | GCNNGC | 1 cut(s) | 609 |
| BstDEI | CTNAG | 3 cut(s) | 135, 494, 558 |
| BstF5I | GGATG | 1 cut(s) | 781 |
| BstFNI | CGCG | 1 cut(s) | 594 |
| BstHHI | GCGC | 2 cut(s) | 267, 286 |
| BstKTI | GATC | 2 cut(s) | 376, 600 |
| BstMAI | GTCTC | 2 cut(s) | 47, 594 |
| BstMBI | GATC | 2 cut(s) | 373, 597 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 242 |
| BstNI | CCWGG | 1 cut(s) | 175 |
| BstSCI | CCNGG | 1 cut(s) | 173 |
| BstSFI | CTRYAG | 1 cut(s) | 10 |
| BstSLI | GKGCMC | 2 cut(s) | 69, 557 |
| BstUI | CGCG | 1 cut(s) | 594 |
| BstV2I | GAAGAC | 1 cut(s) | 317 |
| BstZ17I | GTATAC | 1 cut(s) | 200 |
| BsuRI | GGCC | 1 cut(s) | 364 |
| BtsCI | GGATG | 1 cut(s) | 781 |
| BtsI | GCAGTG | 1 cut(s) | 19 |
| BtsIMutI | CAGTG | 3 cut(s) | 19, 60, 247 |
| Cac8I | GCNNGC | 1 cut(s) | 609 |
| CfoI | GCGC | 2 cut(s) | 267, 286 |
| Cfr13I | GGNCC | 3 cut(s) | 17, 74, 221 |
| Csp6I | GTAC | 5 cut(s) | 127, 213, 628, 687, 759 |
| CviAII | CATG | 2 cut(s) | 158, 586 |
| CviJI | RGCY | 7 cut(s) | 80, 134, 179, 236, 364, 663, 738 |
| CviKI_1 | RGCY | 7 cut(s) | 80, 134, 179, 236, 364, 663, 738 |
| CviQI | GTAC | 5 cut(s) | 127, 213, 628, 687, 759 |
| DdeI | CTNAG | 3 cut(s) | 135, 494, 558 |
| DpnI | GATC | 2 cut(s) | 375, 599 |
| DpnII | GATC | 2 cut(s) | 373, 597 |
| Eam1104I | CTCTTC | 1 cut(s) | 810 |
| EarI | CTCTTC | 1 cut(s) | 810 |
| Eco130I | CCWWGG | 1 cut(s) | 70 |
| Eco47I | GGWCC | 3 cut(s) | 17, 74, 221 |
| Eco57I | CTGAAG | 2 cut(s) | 436, 718 |
| EcoRII | CCWGG | 1 cut(s) | 173 |
| EcoT14I | CCWWGG | 1 cut(s) | 70 |
| ErhI | CCWWGG | 1 cut(s) | 70 |
| Esp3I | CGTCTC | 1 cut(s) | 47 |
| FaeI | CATG | 2 cut(s) | 161, 589 |
| FaqI | GGGAC | 1 cut(s) | 202 |
| FatI | CATG | 2 cut(s) | 157, 585 |
| FauNDI | CATATG | 1 cut(s) | 217 |
| FblI | GTMKAC | 1 cut(s) | 199 |
| FokI | GGATG | 1 cut(s) | 788 |
| FspBI | CTAG | 3 cut(s) | 344, 701, 720 |
| FspI | TGCGCA | 1 cut(s) | 266 |
| GlaI | GCGC | 2 cut(s) | 266, 285 |
| GsuI | CTGGAG | 1 cut(s) | 652 |
| HaeIII | GGCC | 1 cut(s) | 364 |
| HapII | CCGG | 1 cut(s) | 270 |
| HhaI | GCGC | 2 cut(s) | 267, 286 |
| Hin1II | CATG | 2 cut(s) | 161, 589 |
| Hin6I | GCGC | 2 cut(s) | 265, 284 |
| HinP1I | GCGC | 2 cut(s) | 265, 284 |
| HincII | GTYRAC | 1 cut(s) | 717 |
| HindII | GTYRAC | 1 cut(s) | 717 |
| HpaII | CCGG | 1 cut(s) | 270 |
| HphI | GGTGA | 3 cut(s) | 558, 649, 689 |
| Hpy166II | GTNNAC | 6 cut(s) | 127, 200, 296, 426, 555, 717 |
| Hpy188I | TCNGA | 5 cut(s) | 24, 148, 373, 505, 602 |
| Hpy188III | TCNNGA | 1 cut(s) | 344 |
| Hpy8I | GTNNAC | 6 cut(s) | 127, 200, 296, 426, 555, 717 |
| Hpy99I | CGWCG | 1 cut(s) | 754 |
| HpyAV | CCTTC | 2 cut(s) | 380, 801 |
| HpyCH4III | ACNGT | 2 cut(s) | 64, 698 |
| HpyCH4IV | ACGT | 2 cut(s) | 29, 626 |
| HpyCH4V | TGCA | 5 cut(s) | 12, 555, 611, 647, 779 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 242 |
| HpyF3I | CTNAG | 3 cut(s) | 135, 494, 558 |
| HpySE526I | ACGT | 2 cut(s) | 29, 626 |
| Hsp92II | CATG | 2 cut(s) | 161, 589 |
| HspAI | GCGC | 2 cut(s) | 265, 284 |
| Kzo9I | GATC | 2 cut(s) | 373, 597 |
| LweI | GCATC | 2 cut(s) | 634, 766 |
| MaeI | CTAG | 3 cut(s) | 344, 701, 720 |
| MaeII | ACGT | 2 cut(s) | 29, 626 |
| MaeIII | GTNAC | 1 cut(s) | 91 |
| MalI | GATC | 2 cut(s) | 375, 599 |
| MboI | GATC | 2 cut(s) | 373, 597 |
| MboII | GAAGA | 6 cut(s) | 322, 395, 442, 478, 530, 797 |
| MhlI | GDGCHC | 2 cut(s) | 69, 557 |
| MluCI | AATT | 8 cut(s) | 42, 151, 348, 367, 410, 531, 671, 785 |
| MnlI | CCTC | 8 cut(s) | 30, 199, 202, 234, 408, 500, 716, 766 |
| MseI | TTAA | 2 cut(s) | 6, 408 |
| MspI | CCGG | 1 cut(s) | 270 |
| MspR9I | CCNGG | 1 cut(s) | 175 |
| Mva1269I | GAATGC | 1 cut(s) | 609 |
| MvaI | CCWGG | 1 cut(s) | 175 |
| MvnI | CGCG | 1 cut(s) | 594 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 242 |
| NdeI | CATATG | 1 cut(s) | 217 |
| NdeII | GATC | 2 cut(s) | 373, 597 |
| NlaIII | CATG | 2 cut(s) | 161, 589 |
| NmuCI | GTSAC | 1 cut(s) | 91 |
| NsbI | TGCGCA | 1 cut(s) | 266 |
| PctI | GAATGC | 1 cut(s) | 609 |
| Psp6I | CCWGG | 1 cut(s) | 173 |
| PspGI | CCWGG | 1 cut(s) | 173 |
| PspPI | GGNCC | 3 cut(s) | 17, 74, 221 |
| PstI | CTGCAG | 1 cut(s) | 14 |
| RsaI | GTAC | 5 cut(s) | 128, 214, 629, 688, 760 |
| RsaNI | GTAC | 5 cut(s) | 127, 213, 628, 687, 759 |
| SaqAI | TTAA | 2 cut(s) | 6, 408 |
| Sau3AI | GATC | 2 cut(s) | 373, 597 |
| Sau96I | GGNCC | 3 cut(s) | 17, 74, 221 |
| ScaI | AGTACT | 1 cut(s) | 688 |
| ScrFI | CCNGG | 1 cut(s) | 175 |
| SduI | GDGCHC | 2 cut(s) | 69, 557 |
| SfaNI | GCATC | 2 cut(s) | 634, 766 |
| SfcI | CTRYAG | 1 cut(s) | 10 |
| SinI | GGWCC | 3 cut(s) | 17, 74, 221 |
| Sse9I | AATT | 8 cut(s) | 42, 151, 348, 367, 410, 531, 671, 785 |
| SsiI | CCGC | 1 cut(s) | 357 |
| SspMI | CTAG | 3 cut(s) | 344, 701, 720 |
| StyD4I | CCNGG | 1 cut(s) | 173 |
| StyI | CCWWGG | 1 cut(s) | 70 |
| TaaI | ACNGT | 2 cut(s) | 64, 698 |
| TaiI | ACGT | 2 cut(s) | 32, 629 |
| TaqI | TCGA | 1 cut(s) | 816 |
| TaqII | GACCGA | 1 cut(s) | 329 |
| TasI | AATT | 8 cut(s) | 42, 151, 348, 367, 410, 531, 671, 785 |
| TatI | WGTACW | 3 cut(s) | 126, 686, 758 |
| Tru1I | TTAA | 2 cut(s) | 6, 408 |
| Tru9I | TTAA | 2 cut(s) | 6, 408 |
| TscAI | CASTG | 3 cut(s) | 19, 67, 247 |
| TseFI | GTSAC | 1 cut(s) | 91 |
| Tsp45I | GTSAC | 1 cut(s) | 91 |
| TspDTI | ATGAA | 2 cut(s) | 174, 458 |
| TspGWI | ACGGA | 1 cut(s) | 71 |
| TspRI | CASTG | 3 cut(s) | 19, 67, 247 |
| VneI | GTGCAC | 1 cut(s) | 553 |
| VpaK11BI | GGWCC | 3 cut(s) | 17, 74, 221 |
| XapI | RAATTY | 2 cut(s) | 367, 671 |
| XbaI | TCTAGA | 1 cut(s) | 343 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 671 |
| XmiI | GTMKAC | 1 cut(s) | 199 |
| XspI | CTAG | 3 cut(s) | 344, 701, 720 |
| ZrmI | AGTACT | 1 cut(s) | 688 |