Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 341 |
| AccIII | TCCGGA | 2 cut(s) | 133, 307 |
| AciI | CCGC | 3 cut(s) | 14, 81, 84 |
| AcoI | YGGCCR | 2 cut(s) | 213, 217 |
| AcsI | RAATTY | 3 cut(s) | 104, 271, 365 |
| AgeI | ACCGGT | 1 cut(s) | 161 |
| AgsI | TTSAA | 1 cut(s) | 271 |
| AhlI | ACTAGT | 3 cut(s) | 50, 128, 182 |
| AluBI | AGCT | 2 cut(s) | 266, 347 |
| AluI | AGCT | 2 cut(s) | 266, 347 |
| Alw26I | GTCTC | 3 cut(s) | 131, 250, 324 |
| Aor13HI | TCCGGA | 2 cut(s) | 133, 307 |
| AoxI | GGCC | 3 cut(s) | 213, 217, 300 |
| ApeKI | GCWGC | 1 cut(s) | 347 |
| ApoI | RAATTY | 3 cut(s) | 104, 271, 365 |
| AsiGI | ACCGGT | 1 cut(s) | 161 |
| AspS9I | GGNCC | 1 cut(s) | 300 |
| AsuC2I | CCSGG | 3 cut(s) | 18, 94, 212 |
| AsuHPI | GGTGA | 3 cut(s) | 36, 158, 219 |
| BbvI | GCAGC | 1 cut(s) | 334 |
| BcnI | CCSGG | 3 cut(s) | 18, 94, 212 |
| BcoDI | GTCTC | 3 cut(s) | 131, 250, 324 |
| BcuI | ACTAGT | 3 cut(s) | 50, 128, 182 |
| BfaI | CTAG | 3 cut(s) | 51, 129, 183 |
| BisI | GCNGC | 2 cut(s) | 84, 348 |
| BlsI | GCNGC | 2 cut(s) | 85, 349 |
| Bme1390I | CCNGG | 3 cut(s) | 18, 94, 212 |
| BmgT120I | GGNCC | 1 cut(s) | 300 |
| BmiI | GGNNCC | 1 cut(s) | 22 |
| BmrFI | CCNGG | 3 cut(s) | 18, 94, 212 |
| BmsI | GCATC | 1 cut(s) | 83 |
| BpmI | CTGGAG | 2 cut(s) | 224, 363 |
| BpuEI | CTTGAG | 2 cut(s) | 50, 246 |
| BpuMI | CCSGG | 3 cut(s) | 18, 94, 212 |
| BsaI | GGTCTC | 3 cut(s) | 131, 250, 324 |
| BsaJI | CCNNGG | 2 cut(s) | 93, 210 |
| BsaWI | WCCGGW | 8 cut(s) | 121, 133, 140, 161, 168, 229, 307, 326 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 40, 70 |
| Bse118I | RCCGGY | 2 cut(s) | 161, 215 |
| Bse1I | ACTGG | 5 cut(s) | 68, 176, 207, 227, 346 |
| Bse3DI | GCAATG | 1 cut(s) | 320 |
| BseAI | TCCGGA | 2 cut(s) | 133, 307 |
| BseDI | CCNNGG | 2 cut(s) | 93, 210 |
| BseMI | GCAATG | 1 cut(s) | 320 |
| BseNI | ACTGG | 5 cut(s) | 68, 176, 207, 227, 346 |
| BseX3I | CGGCCG | 1 cut(s) | 213 |
| BseXI | GCAGC | 1 cut(s) | 334 |
| Bsh1285I | CGRYCG | 1 cut(s) | 216 |
| BshFI | GGCC | 3 cut(s) | 215, 219, 302 |
| BshTI | ACCGGT | 1 cut(s) | 161 |
| BsiEI | CGRYCG | 1 cut(s) | 216 |
| BslFI | GGGAC | 1 cut(s) | 193 |
| BsmAI | GTCTC | 3 cut(s) | 131, 250, 324 |
| BsmFI | GGGAC | 1 cut(s) | 193 |
| BsnI | GGCC | 3 cut(s) | 215, 219, 302 |
| Bso31I | GGTCTC | 3 cut(s) | 131, 250, 324 |
| Bsp13I | TCCGGA | 2 cut(s) | 133, 307 |
| BspACI | CCGC | 3 cut(s) | 14, 81, 84 |
| BspANI | GGCC | 3 cut(s) | 215, 219, 302 |
| BspEI | TCCGGA | 2 cut(s) | 133, 307 |
| BspLI | GGNNCC | 1 cut(s) | 22 |
| BspTNI | GGTCTC | 3 cut(s) | 131, 250, 324 |
| BsrDI | GCAATG | 1 cut(s) | 320 |
| BsrFI | RCCGGY | 2 cut(s) | 161, 215 |
| BsrI | ACTGG | 5 cut(s) | 68, 176, 207, 227, 346 |
| BssAI | RCCGGY | 2 cut(s) | 161, 215 |
| BssECI | CCNNGG | 2 cut(s) | 93, 210 |
| Bst4CI | ACNGT | 1 cut(s) | 447 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 429 |
| BstC8I | GCNNGC | 1 cut(s) | 217 |
| BstEII | GGTNACC | 2 cut(s) | 164, 225 |
| BstMAI | GTCTC | 3 cut(s) | 131, 250, 324 |
| BstMCI | CGRYCG | 1 cut(s) | 216 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 80, 356, 429 |
| BstPI | GGTNACC | 2 cut(s) | 164, 225 |
| BstSCI | CCNGG | 3 cut(s) | 16, 92, 210 |
| BstV1I | GCAGC | 1 cut(s) | 334 |
| BstZI | CGGCCG | 1 cut(s) | 213 |
| BsuRI | GGCC | 3 cut(s) | 215, 219, 302 |
| BtsIMutI | CAGTG | 3 cut(s) | 200, 220, 339 |
| Cac8I | GCNNGC | 1 cut(s) | 217 |
| Cfr10I | RCCGGY | 2 cut(s) | 161, 215 |
| Cfr13I | GGNCC | 1 cut(s) | 300 |
| CspAI | ACCGGT | 1 cut(s) | 161 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 349, 384 |
| CviAII | CATG | 1 cut(s) | 336 |
| CviJI | RGCY | 7 cut(s) | 23, 39, 215, 219, 266, 302, 347 |
| CviKI_1 | RGCY | 7 cut(s) | 23, 39, 215, 219, 266, 302, 347 |
| EaeI | YGGCCR | 2 cut(s) | 213, 217 |
| EagI | CGGCCG | 1 cut(s) | 213 |
| EciI | GGCGGA | 1 cut(s) | 70 |
| EclXI | CGGCCG | 1 cut(s) | 213 |
| Eco31I | GGTCTC | 3 cut(s) | 131, 250, 324 |
| Eco52I | CGGCCG | 1 cut(s) | 213 |
| Eco91I | GGTNACC | 2 cut(s) | 164, 225 |
| EcoO65I | GGTNACC | 2 cut(s) | 164, 225 |
| EcoRI | GAATTC | 1 cut(s) | 271 |
| FaeI | CATG | 1 cut(s) | 339 |
| FaiI | YATR | 6 cut(s) | 26, 110, 337, 353, 398, 400 |
| FaqI | GGGAC | 1 cut(s) | 193 |
| FatI | CATG | 1 cut(s) | 335 |
| FauNDI | CATATG | 1 cut(s) | 398 |
| Fnu4HI | GCNGC | 2 cut(s) | 84, 348 |
| FseI | GGCCGGCC | 1 cut(s) | 219 |
| Fsp4HI | GCNGC | 2 cut(s) | 84, 348 |
| FspBI | CTAG | 3 cut(s) | 51, 129, 183 |
| GluI | GCNGC | 2 cut(s) | 84, 348 |
| GsuI | CTGGAG | 2 cut(s) | 224, 363 |
| HaeIII | GGCC | 3 cut(s) | 215, 219, 302 |
| Hin1II | CATG | 1 cut(s) | 339 |
| HindIII | AAGCTT | 1 cut(s) | 264 |
| HinfI | GANTC | 8 cut(s) | 118, 144, 151, 172, 193, 206, 233, 295 |
| HphI | GGTGA | 3 cut(s) | 36, 158, 219 |
| Hpy188I | TCNGA | 1 cut(s) | 250 |
| Hpy188III | TCNNGA | 3 cut(s) | 134, 148, 308 |
| HpyCH4III | ACNGT | 1 cut(s) | 447 |
| HpyCH4V | TGCA | 1 cut(s) | 339 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 80, 356, 429 |
| Hsp92II | CATG | 1 cut(s) | 339 |
| Kpn2I | TCCGGA | 2 cut(s) | 133, 307 |
| KroI | GCCGGC | 1 cut(s) | 215 |
| KroNI | GCCGGC | 1 cut(s) | 217 |
| LmnI | GCTCC | 3 cut(s) | 20, 310, 344 |
| Lsp1109I | GCAGC | 1 cut(s) | 334 |
| LweI | GCATC | 1 cut(s) | 83 |
| MaeI | CTAG | 3 cut(s) | 51, 129, 183 |
| MaeIII | GTNAC | 6 cut(s) | 124, 157, 164, 178, 225, 401 |
| MboII | GAAGA | 2 cut(s) | 69, 304 |
| MluCI | AATT | 3 cut(s) | 104, 271, 365 |
| MlyI | GAGTC | 3 cut(s) | 202, 215, 242 |
| MmeI | TCCRAC | 1 cut(s) | 83 |
| MnlI | CCTC | 1 cut(s) | 66 |
| MroI | TCCGGA | 2 cut(s) | 133, 307 |
| MroNI | GCCGGC | 1 cut(s) | 215 |
| MseI | TTAA | 1 cut(s) | 392 |
| MspR9I | CCNGG | 3 cut(s) | 18, 94, 212 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 80, 356, 429 |
| NaeI | GCCGGC | 1 cut(s) | 217 |
| NciI | CCSGG | 3 cut(s) | 18, 94, 212 |
| NdeI | CATATG | 1 cut(s) | 398 |
| NgoMIV | GCCGGC | 1 cut(s) | 215 |
| NlaIII | CATG | 1 cut(s) | 339 |
| NlaIV | GGNNCC | 1 cut(s) | 22 |
| NmuCI | GTSAC | 6 cut(s) | 124, 157, 164, 178, 225, 401 |
| PdiI | GCCGGC | 1 cut(s) | 217 |
| PfeI | GAWTC | 5 cut(s) | 118, 144, 151, 172, 295 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 341 |
| PinAI | ACCGGT | 1 cut(s) | 161 |
| PkrI | GCNGC | 2 cut(s) | 85, 349 |
| PleI | GAGTC | 3 cut(s) | 201, 214, 241 |
| PpsI | GAGTC | 3 cut(s) | 201, 214, 241 |
| PspEI | GGTNACC | 2 cut(s) | 164, 225 |
| PspN4I | GGNNCC | 1 cut(s) | 22 |
| PspPI | GGNCC | 1 cut(s) | 300 |
| RigI | GGCCGGCC | 1 cut(s) | 219 |
| SaqAI | TTAA | 1 cut(s) | 392 |
| SatI | GCNGC | 2 cut(s) | 84, 348 |
| Sau96I | GGNCC | 1 cut(s) | 300 |
| SchI | GAGTC | 3 cut(s) | 202, 215, 242 |
| ScrFI | CCNGG | 3 cut(s) | 18, 94, 212 |
| SetI | ASST | 3 cut(s) | 246, 268, 349 |
| SfaNI | GCATC | 1 cut(s) | 83 |
| SmlI | CTYRAG | 2 cut(s) | 29, 261 |
| SmoI | CTYRAG | 2 cut(s) | 29, 261 |
| SpeI | ACTAGT | 3 cut(s) | 50, 128, 182 |
| Sse9I | AATT | 3 cut(s) | 104, 271, 365 |
| SsiI | CCGC | 3 cut(s) | 14, 81, 84 |
| SspMI | CTAG | 3 cut(s) | 51, 129, 183 |
| StyD4I | CCNGG | 3 cut(s) | 16, 92, 210 |
| TaaI | ACNGT | 1 cut(s) | 447 |
| TasI | AATT | 3 cut(s) | 104, 271, 365 |
| TauI | GCSGC | 1 cut(s) | 86 |
| TfiI | GAWTC | 5 cut(s) | 118, 144, 151, 172, 295 |
| Tru1I | TTAA | 1 cut(s) | 392 |
| Tru9I | TTAA | 1 cut(s) | 392 |
| TscAI | CASTG | 3 cut(s) | 207, 227, 346 |
| TseFI | GTSAC | 6 cut(s) | 124, 157, 164, 178, 225, 401 |
| TseI | GCWGC | 1 cut(s) | 347 |
| Tsp45I | GTSAC | 6 cut(s) | 124, 157, 164, 178, 225, 401 |
| TspDTI | ATGAA | 1 cut(s) | 97 |
| TspRI | CASTG | 3 cut(s) | 207, 227, 346 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 341 |
| XapI | RAATTY | 3 cut(s) | 104, 271, 365 |
| XspI | CTAG | 3 cut(s) | 51, 129, 183 |