Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 24 |
| AcsI | RAATTY | 1 cut(s) | 134 |
| AcuI | CTGAAG | 1 cut(s) | 181 |
| AfaI | GTAC | 3 cut(s) | 151, 223, 301 |
| AgsI | TTSAA | 3 cut(s) | 53, 72, 258 |
| AjnI | CCWGG | 1 cut(s) | 76 |
| AluBI | AGCT | 1 cut(s) | 201 |
| AluI | AGCT | 1 cut(s) | 201 |
| Alw26I | GTCTC | 1 cut(s) | 24 |
| ApoI | RAATTY | 1 cut(s) | 134 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 83, 115 |
| AsuHPI | GGTGA | 2 cut(s) | 112, 152 |
| BccI | CCATC | 2 cut(s) | 137, 226 |
| BciT130I | CCWGG | 1 cut(s) | 78 |
| BcoDI | GTCTC | 1 cut(s) | 24 |
| BfaI | CTAG | 2 cut(s) | 164, 183 |
| BmcAI | AGTACT | 1 cut(s) | 151 |
| Bme1390I | CCNGG | 1 cut(s) | 78 |
| BmrFI | CCNGG | 1 cut(s) | 78 |
| BmsI | GCATC | 2 cut(s) | 97, 229 |
| BpmI | CTGGAG | 1 cut(s) | 115 |
| BsaAI | YACGTR | 1 cut(s) | 303 |
| Bse1I | ACTGG | 2 cut(s) | 98, 157 |
| BseBI | CCWGG | 1 cut(s) | 78 |
| BseGI | GGATG | 1 cut(s) | 244 |
| BseNI | ACTGG | 2 cut(s) | 98, 157 |
| Bsh1236I | CGCG | 1 cut(s) | 24 |
| BsmAI | GTCTC | 1 cut(s) | 24 |
| BsmI | GAATGC | 1 cut(s) | 39 |
| Bsp143I | GATC | 1 cut(s) | 27 |
| BspFNI | CGCG | 1 cut(s) | 24 |
| BsrI | ACTGG | 2 cut(s) | 98, 157 |
| BssMI | GATC | 1 cut(s) | 27 |
| Bst2UI | CCWGG | 1 cut(s) | 78 |
| Bst4CI | ACNGT | 1 cut(s) | 161 |
| Bst6I | CTCTTC | 1 cut(s) | 273 |
| BstBAI | YACGTR | 1 cut(s) | 303 |
| BstC8I | GCNNGC | 1 cut(s) | 39 |
| BstF5I | GGATG | 1 cut(s) | 244 |
| BstFNI | CGCG | 1 cut(s) | 24 |
| BstKTI | GATC | 1 cut(s) | 30 |
| BstMAI | GTCTC | 1 cut(s) | 24 |
| BstMBI | GATC | 1 cut(s) | 27 |
| BstNI | CCWGG | 1 cut(s) | 78 |
| BstSCI | CCNGG | 1 cut(s) | 76 |
| BstUI | CGCG | 1 cut(s) | 24 |
| BtsCI | GGATG | 1 cut(s) | 244 |
| Cac8I | GCNNGC | 1 cut(s) | 39 |
| Csp6I | GTAC | 3 cut(s) | 150, 222, 300 |
| CviJI | RGCY | 3 cut(s) | 76, 126, 201 |
| CviKI_1 | RGCY | 3 cut(s) | 76, 126, 201 |
| CviQI | GTAC | 3 cut(s) | 150, 222, 300 |
| DpnI | GATC | 1 cut(s) | 29 |
| DpnII | GATC | 1 cut(s) | 27 |
| Eam1104I | CTCTTC | 1 cut(s) | 273 |
| EarI | CTCTTC | 1 cut(s) | 273 |
| Eco57I | CTGAAG | 1 cut(s) | 181 |
| EcoRII | CCWGG | 1 cut(s) | 76 |
| FaiI | YATR | 2 cut(s) | 176, 275 |
| FokI | GGATG | 1 cut(s) | 251 |
| FspBI | CTAG | 2 cut(s) | 164, 183 |
| GsuI | CTGGAG | 1 cut(s) | 115 |
| HincII | GTYRAC | 1 cut(s) | 180 |
| HindII | GTYRAC | 1 cut(s) | 180 |
| HinfI | GANTC | 1 cut(s) | 58 |
| HphI | GGTGA | 2 cut(s) | 112, 152 |
| Hpy166II | GTNNAC | 1 cut(s) | 180 |
| Hpy188I | TCNGA | 1 cut(s) | 32 |
| Hpy8I | GTNNAC | 1 cut(s) | 180 |
| Hpy99I | CGWCG | 1 cut(s) | 217 |
| HpyAV | CCTTC | 1 cut(s) | 264 |
| HpyCH4III | ACNGT | 1 cut(s) | 161 |
| HpyCH4IV | ACGT | 1 cut(s) | 302 |
| HpyCH4V | TGCA | 3 cut(s) | 41, 110, 242 |
| HpySE526I | ACGT | 1 cut(s) | 302 |
| Kzo9I | GATC | 1 cut(s) | 27 |
| LpnPI | CCDG | 8 cut(s) | 27, 63, 75, 79, 90, 136, 138, 228 |
| LweI | GCATC | 2 cut(s) | 97, 229 |
| MaeI | CTAG | 2 cut(s) | 164, 183 |
| MaeII | ACGT | 1 cut(s) | 302 |
| MaeIII | GTNAC | 2 cut(s) | 55, 80 |
| MalI | GATC | 1 cut(s) | 29 |
| MboI | GATC | 1 cut(s) | 27 |
| MboII | GAAGA | 1 cut(s) | 260 |
| MluCI | AATT | 2 cut(s) | 134, 248 |
| MlyI | GAGTC | 1 cut(s) | 52 |
| MnlI | CCTC | 3 cut(s) | 24, 179, 229 |
| MspR9I | CCNGG | 1 cut(s) | 78 |
| Mva1269I | GAATGC | 1 cut(s) | 39 |
| MvaI | CCWGG | 1 cut(s) | 78 |
| MvnI | CGCG | 1 cut(s) | 24 |
| NdeII | GATC | 1 cut(s) | 27 |
| NmuCI | GTSAC | 1 cut(s) | 55 |
| PctI | GAATGC | 1 cut(s) | 39 |
| PleI | GAGTC | 1 cut(s) | 52 |
| PpsI | GAGTC | 1 cut(s) | 52 |
| Ppu21I | YACGTR | 1 cut(s) | 303 |
| Psp6I | CCWGG | 1 cut(s) | 76 |
| PspGI | CCWGG | 1 cut(s) | 76 |
| RsaI | GTAC | 3 cut(s) | 151, 223, 301 |
| RsaNI | GTAC | 3 cut(s) | 150, 222, 300 |
| Sau3AI | GATC | 1 cut(s) | 27 |
| ScaI | AGTACT | 1 cut(s) | 151 |
| SchI | GAGTC | 1 cut(s) | 52 |
| ScrFI | CCNGG | 1 cut(s) | 78 |
| SetI | ASST | 4 cut(s) | 16, 203, 256, 305 |
| SfaNI | GCATC | 2 cut(s) | 97, 229 |
| Sse9I | AATT | 2 cut(s) | 134, 248 |
| SspMI | CTAG | 2 cut(s) | 164, 183 |
| StyD4I | CCNGG | 1 cut(s) | 76 |
| TaaI | ACNGT | 1 cut(s) | 161 |
| TaiI | ACGT | 1 cut(s) | 305 |
| TaqI | TCGA | 1 cut(s) | 279 |
| TasI | AATT | 2 cut(s) | 134, 248 |
| TatI | WGTACW | 2 cut(s) | 149, 221 |
| TseFI | GTSAC | 1 cut(s) | 55 |
| Tsp45I | GTSAC | 1 cut(s) | 55 |
| XapI | RAATTY | 1 cut(s) | 134 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 134 |
| XspI | CTAG | 2 cut(s) | 164, 183 |
| ZrmI | AGTACT | 1 cut(s) | 151 |