MD00G1023600.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000088
Physical Location & Seq
Forward (+)
3929608 .. 3937138
7531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1023600.v1.1.491

Sequence Viewer

Length: 2622 bp
ATGCTACATGCCACATCACCAATCTGTATGAGCATGAATTATCTTTACAAACAGGGGGTGTTTCCAGAAGAATTTGTGGCTCTGAGATATCTCACATCTTTGAGAATCAGTCAAAATTATTTCACCGGTCCCCTACCGGCATTCATTGGCAATATGTCTGCATTGACTGAATTGTCAATTAATGTCAATTCATTCTCTGGGCCCATCCCCAAGGAGCTTGGAAACCTTAAGGAGCTAACTCTGCTGGACATCGAATCAAATAATTTCTTCGGAACACTCCCTCCAAAACTTGGTAATTTAGTCAAGCTCGAGCGACTGTATATGGATAAGAATTATTTCACCGGTCCCCTACCGGCATTCATTGGCAATATGTCTGCATTGACTGGATTTTACATGGACAGCTGTGGACTCGGTGGTAAAATTCCTTCAACATTTGCCAAGCTCACCAACATGCAATACCTCTCGGCATCGGACAATCCTTTCTCAGGAAAGATACCTGATTTCATAGGGAATTGGACACAACTCACTGATTTGGAATTTCAAGGGAACTCCTTCGAAGGCCCAATACCAACCAGCTTTTCTCAACTGACCTCATTGGAGTATCTGGATCTGAGTTTCAACAATTTGACAAGCCAACTCCCAAGTTCTTTGTTCAGCTTGAGTTCTCTTACATCCTTAGATTTGTCTTACAATTTTTTATCAGGAAGCTTTCCCCAGTGGGTGACCAATATATCGCAAGTGAACTTAGTGGTCAACAACTTCACATTTGACAATTCAAACATAACTCTTCCTGGATTGAATTGCCTCCAGAGAAATTTTCCATGCAATCGAAATACCCCACGATATGCAAGCTTCTCAATCAACTGTGGTGGACAGCAAATGAAGGGAGATGGCATATTGTATGAGACTGATGAATCAGTTCTTGGCTCAGCAACATTCAGTGTAACCAGTGCAGAGAATTGGGCAGTCAGCAATGCCGGTTTGTTTTCTGCCCAATATACCTCATCGTCTCGGGAAAATAACCCATCGTTGGAAAATACCCATGCACAAGTCAGCGGAACAGACGTGACCCCGGAGCTTTTCCAGACTTCAAGAGTGTCCAGAGGATCACTGAGATACTATGGCCTGGGCCTTGAGAATGGGCCCTACACTGTAACATTGCACTTTGCAGAGACGGTTTATGAAAGTCGCACTTCGCAAACTTGGAAAAGTCTAGGACGGCGTGTATTTGATATCTATATTCAGGGTACCCGCAGGATGAAGGACTTTGACATATCGAAAGAGGCAGGTGGTGTTAACCGAGCAGTTGTGAGAAAATTTAATGTTAGCGTGTCAGAGAATTATCTTGAAATTCATTTGTTCTGGGCTGGTAAAGGGACTTGTTGCATACCCAGTTATAATGATTACGGCCCACTAATAGCAGCCGTCCATGCTGCTTCAGATTTTACAAAGAAGAGCAATACTGGGTTGATAGTTGGTATTCCAGTTCTTGTTGGAGTTGTGAGCTTGCTATTAATATTTGCAATTCTATATATGAGGAGGAAAAAATCAGAAAAAGAAGACGATGAAGATATTCTAGGATTAGGCCCCCGGCCATATACTTTCAGTTATGTTGAATTAAGAACTGCAACTGAAGATTTTAATCCTTCAAATAAGCTAGGAGAGGGAGGATATGGCCCCGTTTATAAGGGTACACTTTCTGATGGTAGAGTAGTGGCTGTGAAGCAACTTTCAGTATCATCTCACCAAGGGAAGAGTCAATTTGTATCTGAAATTGCTACCATATCTGCTGTGCAACATCGGAATCTAGTGAAATTGTATGGATGTTGCATTGAAGGCAGACACCGCATTTTGGTTTATGAGTATCTTGAAAACAAGAGCCTTGATCAGGCACTTTTTGGAACAAGTAACTTGCACCTTGACTGGCCTACTCGATTCAATATATTGTTGGGAACAGCAAGAGGATTTGCTTACCTTCATGAGGAGTCAAGGCCAAGGATTGTACATCGAGATGTCAAAGCGAGTAATATTTTGCTTGATGCAGAACTCTCCCCAAAAATATCAGATTTTGGACTGGCAAAGCTTTATGATGACGAGAAAACCCACATCAGCACCCGGGTTGCAGGGACAATAAGCTATTTGGCACCGGAGTATGCAATGTTTGGACATTTGACAGAGAAGGTCGATGTGTTTGGTTTTGGAGTCGTCGTTTTAGAGATCCTCAGCGGGAGACCAAACTCTTATAATAACTTACATCCAGAAAAGATTTCTCTTATTGAATGGGTGTGGACTCTACATGAAAACGACCAAACTCTAGGGCTGGTGGATCCAAGATTGACAAAGTTTGATGAAACTGAAGCAACTAGATTGATAAGAGTAGCTCTCATGTGCACGCAGGGATCACCGATGGCACGGCCATCTATGTCACGCGTGGTTGCAATGCTCTCTGGAGACATTGACATAGGCACTGTCATGTCGAAGCCAAGCTATTTGACAGATTATGATTTTAAAGATGTAACAACATCGTTAACAAGTAGATTTTTGGTGGAGGATGATACCCCATCAACTTCATCCAAGGGTATGGTGCTAACGCTCCCTGGATTGACCATGCACCTTCTGTAA

Protein Analysis

874

Amino Acids

96.8

Weight (kDa)

5.88

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 21 - 86 2.1e-06 Leucine-rich repeat region
LRR_14 PF23598 69 - 128 7.8e-07 Leucine-rich repeat region
LRR_14 PF23598 97 - 244 1.5e-09 Leucine-rich repeat region
LRR_8 PF13855 174 - 231 3.4e-08 Leucine rich repeat
Malectin PF11721 285 - 476 9.9e-39 Malectin domain
Pkinase PF00069 549 - 812 1.7e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 550 - 814 8.4e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1398, 1686, 2244
AarI CACCTGC 1 cut(s) 1277
AasI GACNNNNNNGTC 1 cut(s) 172
Acc36I ACCTGC 1 cut(s) 1277
Acc65I GGTACC 1 cut(s) 1247
AccB1I GGYRCC 2 cut(s) 1247, 2143
AccB7I CCANNNNNTGG 1 cut(s) 290
AccII CGCG 1 cut(s) 2430
AciI CCGC 4 cut(s) 1056, 1252, 1846, 2226
AclWI GGATC 6 cut(s) 615, 1114, 2212, 2321, 2334, 2407
AcoI YGGCCR 2 cut(s) 1592, 2414
AcsI RAATTY 6 cut(s) 71, 420, 536, 814, 1316, 1350
AcuI CTGAAG 3 cut(s) 1422, 1653, 2376
AfaI GTAC 3 cut(s) 1249, 1693, 2004
AfiI CCNNNNNNNGG 6 cut(s) 290, 485, 1030, 1852, 1888, 1981
AflII CTTAAG 1 cut(s) 227
AflIII ACRYGT 1 cut(s) 2428
AgeI ACCGGT 2 cut(s) 125, 341
AjiI CACGTC 1 cut(s) 1066
AjnI CCWGG 3 cut(s) 790, 1125, 2596
AjuI GAANNNNNNNTTGG 4 cut(s) 562, 594, 906, 938
AloI GAACNNNNNNTCC 2 cut(s) 265, 297
Alw21I GWGCWC 1 cut(s) 2393
Alw26I GTCTC 5 cut(s) 899, 1014, 1166, 2224, 2445
Alw44I GTGCAC 1 cut(s) 2389
AlwI GGATC 6 cut(s) 615, 1114, 2212, 2321, 2334, 2407
Ama87I CYCGRG 3 cut(s) 308, 1011, 2115
ApaI GGGCCC 2 cut(s) 204, 1146
ApaLI GTGCAC 1 cut(s) 2389
ApeKI GCWGC 2 cut(s) 1421, 1433
ApoI RAATTY 6 cut(s) 71, 420, 536, 814, 1316, 1350
AseI ATTAAT 2 cut(s) 180, 1514
AsiGI ACCGGT 2 cut(s) 125, 341
Asp700I GAANNNNTTC 4 cut(s) 335, 551, 918, 1572
Asp718I GGTACC 1 cut(s) 1247
AsuC2I CCSGG 4 cut(s) 1073, 1591, 2116, 2117
AsuHPI GGTGA 7 cut(s) 9, 115, 331, 436, 733, 1736, 2394
AsuII TTCGAA 1 cut(s) 555
AvaI CYCGRG 3 cut(s) 308, 1011, 2115
AvaII GGWCC 2 cut(s) 128, 344
BaeGI GKGCMC 3 cut(s) 204, 1146, 2393
BamHI GGATCC 1 cut(s) 2326
BanI GGYRCC 2 cut(s) 1247, 2143
BanII GRGCYC 2 cut(s) 204, 1146
BarI GAAGNNNNNNTAC 4 cut(s) 409, 441, 549, 581
BbsI GAAGAC 1 cut(s) 1566
Bbv12I GWGCWC 1 cut(s) 2393
BbvCI CCTCAGC 1 cut(s) 2222
BbvI GCAGC 2 cut(s) 1420, 1433
BccI CCATC 7 cut(s) 212, 884, 1033, 1697, 2401, 2425, 2569
BceAI ACGGC 4 cut(s) 1235, 1409, 1423, 2429
BciT130I CCWGG 3 cut(s) 792, 1127, 2598
BclI TGATCA 1 cut(s) 1885
BcnI CCSGG 4 cut(s) 1073, 1591, 2116, 2117
BcoDI GTCTC 5 cut(s) 899, 1014, 1166, 2224, 2445
BfaI CTAG 6 cut(s) 1214, 1577, 1658, 1808, 2315, 2364
BfrI CTTAAG 1 cut(s) 227
BfuAI ACCTGC 1 cut(s) 1277
BisI GCNGC 2 cut(s) 1422, 1434
BlpI GCTNAGC 1 cut(s) 928
BlsI GCNGC 2 cut(s) 1423, 1435
Bme1390I CCNGG 7 cut(s) 792, 1073, 1127, 1591, 2116, 2117, 2598
Bme18I GGWCC 2 cut(s) 128, 344
BmeT110I CYCGRG 3 cut(s) 308, 1011, 2115
BmgBI CACGTC 1 cut(s) 1066
BmiI GGNNCC 9 cut(s) 130, 202, 346, 1144, 1249, 1588, 1678, 2145, 2328
BmrFI CCNGG 7 cut(s) 792, 1073, 1127, 1591, 2116, 2117, 2598
BmrI ACTGGG 3 cut(s) 709, 1386, 1473
BmsI GCATC 2 cut(s) 476, 2029
BmuI ACTGGG 3 cut(s) 709, 1386, 1473
BpiI GAAGAC 1 cut(s) 1566
BplI GAGNNNNNCTC 2 cut(s) 2367, 2399
BpmI CTGGAG 2 cut(s) 791, 2469
Bpu10I CCTNAGC 1 cut(s) 2222
Bpu1102I GCTNAGC 1 cut(s) 928
Bpu14I TTCGAA 1 cut(s) 555
BpuEI CTTGAG 2 cut(s) 679, 1154
BpuMI CCSGG 4 cut(s) 1073, 1591, 2116, 2117
BsaBI GATNNNNATC 1 cut(s) 1641
BsaI GGTCTC 1 cut(s) 2224
BsaJI CCNNGG 9 cut(s) 210, 1071, 1126, 1589, 1747, 1994, 2115, 2574, 2596
BsaWI WCCGGW 3 cut(s) 125, 341, 2146
BsaXI ACNNNNNCTCC 4 cut(s) 265, 295, 2193, 2223
Bsc4I CCNNNNNNNGG 6 cut(s) 290, 485, 1030, 1852, 1888, 1981
Bse118I RCCGGY 5 cut(s) 125, 136, 341, 352, 977
Bse1I ACTGG 8 cut(s) 388, 715, 948, 1392, 1468, 1484, 1928, 2079
Bse3DI GCAATG 4 cut(s) 979, 1157, 2163, 2445
Bse8I GATNNNNATC 1 cut(s) 1641
BseBI CCWGG 3 cut(s) 792, 1127, 2598
BseDI CCNNGG 9 cut(s) 210, 1071, 1126, 1589, 1747, 1994, 2115, 2574, 2596
BseGI GGATG 7 cut(s) 204, 671, 1263, 1829, 2254, 2557, 2570
BseJI GATNNNNATC 1 cut(s) 1641
BseLI CCNNNNNNNGG 6 cut(s) 290, 485, 1030, 1852, 1888, 1981
BseMI GCAATG 4 cut(s) 979, 1157, 2163, 2445
BseMII CTCAG 6 cut(s) 74, 498, 602, 942, 1103, 2236
BseNI ACTGG 8 cut(s) 388, 715, 948, 1392, 1468, 1484, 1928, 2079
BseRI GAGGAG 2 cut(s) 1552, 1997
BseSI GKGCMC 3 cut(s) 204, 1146, 2393
BseXI GCAGC 2 cut(s) 1420, 1433
BsgI GTGCAG 1 cut(s) 972
Bsh1236I CGCG 1 cut(s) 2430
BshNI GGYRCC 2 cut(s) 1247, 2143
BshTI ACCGGT 2 cut(s) 125, 341
BsiHKAI GWGCWC 1 cut(s) 2393
BsiHKCI CYCGRG 3 cut(s) 308, 1011, 2115
BsiSI CCGG 9 cut(s) 126, 137, 342, 353, 978, 1073, 1591, 2116, 2147
BslFI GGGAC 4 cut(s) 114, 330, 1390, 2140
BslI CCNNNNNNNGG 6 cut(s) 290, 485, 1030, 1852, 1888, 1981
BsmAI GTCTC 5 cut(s) 899, 1014, 1166, 2224, 2445
BsmBI CGTCTC 2 cut(s) 1014, 1166
BsmFI GGGAC 4 cut(s) 114, 330, 1390, 2140
BsmI GAATGC 2 cut(s) 140, 356
Bso31I GGTCTC 1 cut(s) 2224
BsoBI CYCGRG 3 cut(s) 308, 1011, 2115
Bsp119I TTCGAA 1 cut(s) 555
Bsp120I GGGCCC 2 cut(s) 200, 1142
Bsp1286I GDGCHC 3 cut(s) 204, 1146, 2393
Bsp1407I TGTACA 1 cut(s) 2002
Bsp143I GATC 6 cut(s) 607, 1106, 1885, 2217, 2326, 2399
Bsp1720I GCTNAGC 1 cut(s) 928
BspACI CCGC 4 cut(s) 1056, 1252, 1846, 2226
BspCNI CTCAG 6 cut(s) 75, 497, 603, 941, 1104, 2235
BspFNI CGCG 1 cut(s) 2430
BspHI TCATGA 1 cut(s) 1978
BspLI GGNNCC 9 cut(s) 130, 202, 346, 1144, 1249, 1588, 1678, 2145, 2328
BspMI ACCTGC 1 cut(s) 1277
BspPI GGATC 6 cut(s) 615, 1114, 2212, 2321, 2334, 2407
BspQI GCTCTTC 1 cut(s) 1448
BspT104I TTCGAA 1 cut(s) 555
BspT107I GGYRCC 2 cut(s) 1247, 2143
BspTI CTTAAG 1 cut(s) 227
BspTNI GGTCTC 1 cut(s) 2224
BsrDI GCAATG 4 cut(s) 979, 1157, 2163, 2445
BsrFI RCCGGY 5 cut(s) 125, 136, 341, 352, 977
BsrGI TGTACA 1 cut(s) 2002
BsrI ACTGG 8 cut(s) 388, 715, 948, 1392, 1468, 1484, 1928, 2079
BssAI RCCGGY 5 cut(s) 125, 136, 341, 352, 977
BssECI CCNNGG 9 cut(s) 210, 1071, 1126, 1589, 1747, 1994, 2115, 2574, 2596
BssMI GATC 6 cut(s) 607, 1106, 1885, 2217, 2326, 2399
BssT1I CCWWGG 4 cut(s) 210, 1747, 1994, 2574
Bst2UI CCWGG 3 cut(s) 792, 1127, 2598
Bst4CI ACNGT 5 cut(s) 318, 866, 1153, 1177, 2470
Bst6I CTCTTC 3 cut(s) 792, 1448, 1748
BstAFI CTTAAG 1 cut(s) 227
BstAUI TGTACA 1 cut(s) 2002
BstBI TTCGAA 1 cut(s) 555
BstC8I GCNNGC 3 cut(s) 850, 1508, 2393
BstDEI CTNAG 8 cut(s) 83, 484, 611, 676, 745, 928, 1112, 2222
BstEII GGTNACC 1 cut(s) 721
BstENI CCTNNNNNAGG 3 cut(s) 483, 1886, 1979
BstF5I GGATG 7 cut(s) 204, 671, 1263, 1829, 2254, 2557, 2570
BstFNI CGCG 1 cut(s) 2430
BstKTI GATC 6 cut(s) 610, 1109, 1888, 2220, 2329, 2402
BstMAI GTCTC 5 cut(s) 899, 1014, 1166, 2224, 2445
BstMBI GATC 6 cut(s) 607, 1106, 1885, 2217, 2326, 2399
BstMWI GCNNNNNNNGC 4 cut(s) 241, 1430, 1836, 1845
BstNI CCWGG 3 cut(s) 792, 1127, 2598
BstNSI RCATGY 2 cut(s) 11, 454
BstPI GGTNACC 1 cut(s) 721
BstSCI CCNGG 7 cut(s) 790, 1071, 1125, 1589, 2114, 2115, 2596
BstSLI GKGCMC 3 cut(s) 204, 1146, 2393
BstUI CGCG 1 cut(s) 2430
BstV1I GCAGC 2 cut(s) 1420, 1433
BstV2I GAAGAC 1 cut(s) 1566
BstX2I RGATCY 3 cut(s) 607, 2217, 2326
BstXI CCANNNNNNTGG 1 cut(s) 2581
BstYI RGATCY 3 cut(s) 607, 2217, 2326
BtrI CACGTC 1 cut(s) 1066
BtsCI GGATG 7 cut(s) 204, 671, 1263, 1829, 2254, 2557, 2570
BtsIMutI CAGTG 7 cut(s) 525, 722, 946, 955, 1109, 1149, 2466
BveI ACCTGC 1 cut(s) 1277
Cac8I GCNNGC 3 cut(s) 850, 1508, 2393
CciI TCATGA 1 cut(s) 1978
Cfr10I RCCGGY 5 cut(s) 125, 136, 341, 352, 977
Cfr9I CCCGGG 1 cut(s) 2115
Csp6I GTAC 3 cut(s) 1248, 1692, 2003
CspAI ACCGGT 2 cut(s) 125, 341
CspCI CAANNNNNGTGG 2 cut(s) 850, 885
CviQI GTAC 3 cut(s) 1248, 1692, 2003
DdeI CTNAG 8 cut(s) 83, 484, 611, 676, 745, 928, 1112, 2222
DpnI GATC 6 cut(s) 609, 1108, 1887, 2219, 2328, 2401
DpnII GATC 6 cut(s) 607, 1106, 1885, 2217, 2326, 2399
DraI TTTAAA 1 cut(s) 2509
DrdI GACNNNNNNGTC 1 cut(s) 172
DseDI GACNNNNNNGTC 1 cut(s) 172
EaeI YGGCCR 2 cut(s) 1592, 2414
Eam1104I CTCTTC 3 cut(s) 792, 1448, 1748
EarI CTCTTC 3 cut(s) 792, 1448, 1748
Eco130I CCWWGG 4 cut(s) 210, 1747, 1994, 2574
Eco24I GRGCYC 2 cut(s) 204, 1146
Eco31I GGTCTC 1 cut(s) 2224
Eco32I GATATC 2 cut(s) 89, 1234
Eco47I GGWCC 2 cut(s) 128, 344
Eco57I CTGAAG 3 cut(s) 1422, 1653, 2376
Eco88I CYCGRG 3 cut(s) 308, 1011, 2115
Eco91I GGTNACC 1 cut(s) 721
EcoNI CCTNNNNNAGG 3 cut(s) 483, 1886, 1979
EcoO109I RGGNCCY 2 cut(s) 1143, 1586
EcoO65I GGTNACC 1 cut(s) 721
EcoRII CCWGG 3 cut(s) 790, 1125, 2596
EcoRV GATATC 2 cut(s) 89, 1234
EcoT14I CCWWGG 4 cut(s) 210, 1747, 1994, 2574
EcoT38I GRGCYC 2 cut(s) 204, 1146
ErhI CCWWGG 4 cut(s) 210, 1747, 1994, 2574
Esp3I CGTCTC 2 cut(s) 1014, 1166
FalI AAGNNNNNCTT 2 cut(s) 1177, 1209
FaqI GGGAC 4 cut(s) 114, 330, 1390, 2140
FauI CCCGC 2 cut(s) 1259, 2219
FbaI TGATCA 1 cut(s) 1885
Fnu4HI GCNGC 2 cut(s) 1422, 1434
FokI GGATG 7 cut(s) 191, 658, 1270, 1836, 2241, 2557, 2564
FriOI GRGCYC 2 cut(s) 204, 1146
Fsp4HI GCNGC 2 cut(s) 1422, 1434
FspBI CTAG 6 cut(s) 1214, 1577, 1658, 1808, 2315, 2364
GluI GCNGC 2 cut(s) 1422, 1434
GsuI CTGGAG 2 cut(s) 791, 2469
HapII CCGG 9 cut(s) 126, 137, 342, 353, 978, 1073, 1591, 2116, 2147
HincII GTYRAC 3 cut(s) 754, 1297, 2529
HindII GTYRAC 3 cut(s) 754, 1297, 2529
HindIII AAGCTT 3 cut(s) 706, 850, 2081
HpaI GTTAAC 2 cut(s) 1297, 2529
HpaII CCGG 9 cut(s) 126, 137, 342, 353, 978, 1073, 1591, 2116, 2147
HphI GGTGA 7 cut(s) 9, 115, 331, 436, 733, 1736, 2394
Hpy166II GTNNAC 9 cut(s) 407, 742, 754, 872, 1297, 1694, 2289, 2391, 2529
Hpy8I GTNNAC 9 cut(s) 407, 742, 754, 872, 1297, 1694, 2289, 2391, 2529
Hpy99I CGWCG 1 cut(s) 2210
HpyAV CCTTC 9 cut(s) 435, 551, 562, 877, 1255, 1656, 1829, 1985, 2173
HpyCH4III ACNGT 5 cut(s) 318, 866, 1153, 1177, 2470
HpyCH4IV ACGT 1 cut(s) 1065
HpyF10VI GCNNNNNNNGC 4 cut(s) 241, 1430, 1836, 1845
HpyF3I CTNAG 8 cut(s) 83, 484, 611, 676, 745, 928, 1112, 2222
HpySE526I ACGT 1 cut(s) 1065
KpnI GGTACC 1 cut(s) 1251
Ksp22I TGATCA 1 cut(s) 1885
KspAI GTTAAC 2 cut(s) 1297, 2529
Kzo9I GATC 6 cut(s) 607, 1106, 1885, 2217, 2326, 2399
LguI GCTCTTC 1 cut(s) 1448
LmnI GCTCC 4 cut(s) 214, 232, 1075, 2598
Lsp1109I GCAGC 2 cut(s) 1420, 1433
LweI GCATC 2 cut(s) 476, 2029
MaeI CTAG 6 cut(s) 1214, 1577, 1658, 1808, 2315, 2364
MaeII ACGT 1 cut(s) 1065
MaeIII GTNAC 7 cut(s) 721, 943, 1066, 1153, 1907, 2424, 2515
MalI GATC 6 cut(s) 609, 1108, 1887, 2219, 2328, 2401
MboI GATC 6 cut(s) 607, 1106, 1885, 2217, 2326, 2399
MboII GAAGA 8 cut(s) 80, 259, 779, 1465, 1571, 1580, 1646, 1765
MflI RGATCY 3 cut(s) 607, 2217, 2326
MhlI GDGCHC 3 cut(s) 204, 1146, 2393
MluI ACGCGT 1 cut(s) 2428
MlyI GAGTC 5 cut(s) 402, 1765, 1994, 2211, 2284
MmeI TCCRAC 2 cut(s) 1011, 1474
MroXI GAANNNNTTC 4 cut(s) 335, 551, 918, 1572
MseI TTAA 9 cut(s) 180, 228, 1296, 1320, 1514, 1619, 1641, 2508, 2528
MslI CAYNNNNRTG 3 cut(s) 449, 2010, 2471
MspA1I CMGCKG 3 cut(s) 402, 1056, 2226
MspCI CTTAAG 1 cut(s) 227
MspI CCGG 9 cut(s) 126, 137, 342, 353, 978, 1073, 1591, 2116, 2147
MspR9I CCNGG 7 cut(s) 792, 1073, 1127, 1591, 2116, 2117, 2598
Mva1269I GAATGC 2 cut(s) 140, 356
MvaI CCWGG 3 cut(s) 792, 1127, 2598
MvnI CGCG 1 cut(s) 2430
MwoI GCNNNNNNNGC 4 cut(s) 241, 1430, 1836, 1845
NciI CCSGG 4 cut(s) 1073, 1591, 2116, 2117
NdeII GATC 6 cut(s) 607, 1106, 1885, 2217, 2326, 2399
NlaIV GGNNCC 9 cut(s) 130, 202, 346, 1144, 1249, 1588, 1678, 2145, 2328
NmeAIII GCCGAG 1 cut(s) 443
NmuCI GTSAC 3 cut(s) 721, 1066, 2424
NspI RCATGY 2 cut(s) 11, 454
NspV TTCGAA 1 cut(s) 555
PaeR7I CTCGAG 1 cut(s) 308
PagI TCATGA 1 cut(s) 1978
PaqCI CACCTGC 1 cut(s) 1277
PciSI GCTCTTC 1 cut(s) 1448
PctI GAATGC 2 cut(s) 140, 356
PdmI GAANNNNTTC 4 cut(s) 335, 551, 918, 1572
PfeI GAWTC 5 cut(s) 105, 254, 914, 1804, 1935
PflMI CCANNNNNTGG 1 cut(s) 290
PfoI TCCNGGA 1 cut(s) 790
PinAI ACCGGT 2 cut(s) 125, 341
PkrI GCNGC 2 cut(s) 1423, 1435
PleI GAGTC 5 cut(s) 402, 1764, 1993, 2210, 2284
PpsI GAGTC 5 cut(s) 402, 1764, 1993, 2210, 2284
PshBI ATTAAT 2 cut(s) 180, 1514
PsiI TTATAA 3 cut(s) 1398, 1686, 2244
Psp6I CCWGG 3 cut(s) 790, 1125, 2596
PspEI GGTNACC 1 cut(s) 721
PspGI CCWGG 3 cut(s) 790, 1125, 2596
PspN4I GGNNCC 9 cut(s) 130, 202, 346, 1144, 1249, 1588, 1678, 2145, 2328
PspOMI GGGCCC 2 cut(s) 200, 1142
PspXI VCTCGAGB 1 cut(s) 308
PsuI RGATCY 3 cut(s) 607, 2217, 2326
PvuII CAGCTG 1 cut(s) 402
RsaI GTAC 3 cut(s) 1249, 1693, 2004
RsaNI GTAC 3 cut(s) 1248, 1692, 2003
RseI CAYNNNNRTG 3 cut(s) 449, 2010, 2471
SapI GCTCTTC 1 cut(s) 1448
SaqAI TTAA 9 cut(s) 180, 228, 1296, 1320, 1514, 1619, 1641, 2508, 2528
SatI GCNGC 2 cut(s) 1422, 1434
Sau3AI GATC 6 cut(s) 607, 1106, 1885, 2217, 2326, 2399
SchI GAGTC 5 cut(s) 402, 1765, 1994, 2211, 2284
ScrFI CCNGG 7 cut(s) 792, 1073, 1127, 1591, 2116, 2117, 2598
SduI GDGCHC 3 cut(s) 204, 1146, 2393
SfaNI GCATC 2 cut(s) 476, 2029
Sfr274I CTCGAG 1 cut(s) 308
SfuI TTCGAA 1 cut(s) 555
SinI GGWCC 2 cut(s) 128, 344
SlaI CTCGAG 1 cut(s) 308
SmaI CCCGGG 1 cut(s) 2117
SmiMI CAYNNNNRTG 3 cut(s) 449, 2010, 2471
SmlI CTYRAG 4 cut(s) 227, 308, 658, 1133
SmoI CTYRAG 4 cut(s) 227, 308, 658, 1133
SsiI CCGC 4 cut(s) 1056, 1252, 1846, 2226
SspI AATATT 2 cut(s) 1518, 2029
SspMI CTAG 6 cut(s) 1214, 1577, 1658, 1808, 2315, 2364
StyD4I CCNGG 7 cut(s) 790, 1071, 1125, 1589, 2114, 2115, 2596
StyI CCWWGG 4 cut(s) 210, 1747, 1994, 2574
TaaI ACNGT 5 cut(s) 318, 866, 1153, 1177, 2470
TaiI ACGT 1 cut(s) 1068
TaqI TCGA 9 cut(s) 252, 309, 555, 829, 1277, 1933, 2008, 2184, 2477
TatI WGTACW 1 cut(s) 2002
TfiI GAWTC 5 cut(s) 105, 254, 914, 1804, 1935
Tru1I TTAA 9 cut(s) 180, 228, 1296, 1320, 1514, 1619, 1641, 2508, 2528
Tru9I TTAA 9 cut(s) 180, 228, 1296, 1320, 1514, 1619, 1641, 2508, 2528
TscAI CASTG 7 cut(s) 532, 722, 946, 955, 1116, 1156, 2473
TseFI GTSAC 3 cut(s) 721, 1066, 2424
TseI GCWGC 2 cut(s) 1421, 1433
Tsp45I GTSAC 3 cut(s) 721, 1066, 2424
TspMI CCCGGG 1 cut(s) 2115
TspRI CASTG 7 cut(s) 532, 722, 946, 955, 1116, 1156, 2473
Van91I CCANNNNNTGG 1 cut(s) 290
Vha464I CTTAAG 1 cut(s) 227
VneI GTGCAC 1 cut(s) 2389
VpaK11BI GGWCC 2 cut(s) 128, 344
VspI ATTAAT 2 cut(s) 180, 1514
XagI CCTNNNNNAGG 3 cut(s) 483, 1886, 1979
XapI RAATTY 6 cut(s) 71, 420, 536, 814, 1316, 1350
XceI RCATGY 2 cut(s) 11, 454
XhoI CTCGAG 1 cut(s) 308
XmaI CCCGGG 1 cut(s) 2115
XmnI GAANNNNTTC 4 cut(s) 335, 551, 918, 1572
XspI CTAG 6 cut(s) 1214, 1577, 1658, 1808, 2315, 2364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.