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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 23 |
| AdeI | CACNNNGTG | 1 cut(s) | 103 |
| ApoI | RAATTY | 1 cut(s) | 23 |
| BfaI | CTAG | 2 cut(s) | 57, 172 |
| BsaJI | CCNNGG | 1 cut(s) | 127 |
| BseDI | CCNNGG | 1 cut(s) | 127 |
| BssECI | CCNNGG | 1 cut(s) | 127 |
| BssT1I | CCWWGG | 1 cut(s) | 127 |
| Bst4CI | ACNGT | 1 cut(s) | 118 |
| Bst6I | CTCTTC | 2 cut(s) | 78, 149 |
| CviJI | RGCY | 1 cut(s) | 163 |
| CviKI_1 | RGCY | 1 cut(s) | 163 |
| DraIII | CACNNNGTG | 1 cut(s) | 103 |
| Eam1104I | CTCTTC | 2 cut(s) | 78, 149 |
| EarI | CTCTTC | 2 cut(s) | 78, 149 |
| Eco130I | CCWWGG | 1 cut(s) | 127 |
| EcoT14I | CCWWGG | 1 cut(s) | 127 |
| ErhI | CCWWGG | 1 cut(s) | 127 |
| FaiI | YATR | 3 cut(s) | 62, 67, 113 |
| FspBI | CTAG | 2 cut(s) | 57, 172 |
| FspEI | CC | 6 cut(s) | 2, 18, 114, 117, 130, 140 |
| HinfI | GANTC | 2 cut(s) | 123, 135 |
| HpyCH4III | ACNGT | 1 cut(s) | 118 |
| HpyCH4V | TGCA | 1 cut(s) | 50 |
| MaeI | CTAG | 2 cut(s) | 57, 172 |
| MboII | GAAGA | 3 cut(s) | 32, 65, 136 |
| MluCI | AATT | 2 cut(s) | 23, 87 |
| PfeI | GAWTC | 2 cut(s) | 123, 135 |
| SetI | ASST | 2 cut(s) | 37, 133 |
| SgeI | CNNG | 2 cut(s) | 69, 140 |
| Sse9I | AATT | 2 cut(s) | 23, 87 |
| SspMI | CTAG | 2 cut(s) | 57, 172 |
| StyI | CCWWGG | 1 cut(s) | 127 |
| TaaI | ACNGT | 1 cut(s) | 118 |
| TasI | AATT | 2 cut(s) | 23, 87 |
| TfiI | GAWTC | 2 cut(s) | 123, 135 |
| TspDTI | ATGAA | 1 cut(s) | 65 |
| XapI | RAATTY | 1 cut(s) | 23 |
| XspI | CTAG | 2 cut(s) | 57, 172 |