MD00G1183000.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000088
Physical Location & Seq
Forward (+)
43283723 .. 43289953
6231 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1183000.v1.1.491

Sequence Viewer

Length: 2760 bp
ATGCTACATGCCACATCACCCAATTGTATGAGAGTGTATGCTCTTAACAAACGAGGGGTGTTTCCAGAAGAATTTGTGGCTTTGAGATATCTCACATATTTGAGTATCTATCAGAATTATTTCACCGGTCCCCTACCGGCATTCATTGGCAATATGTCTGCATTGACTCACTTGTCAATTGGCGTCAATTCATTCTCTGAGCCCATCCCCAAGGAGCTTGGAAACCTTAAGGAGCTAACATATTTGGACATTGGATCAAATAATTTCTCCGGAACACTCCCTCCAGAACTTGGTAATTTAGTCAAGCTCGAGCAACTTTACATGGACAGCTGTGGACTCGGTGGTGAAATTCCTTCAACATTTGCCAACCTCACCAACATGCAAGTCGTCTGGGCATCGGACAATCCTTTCTCGGGAAAGATACCTGATTTCATAGGGAATTGGACACAACTCTATCATTTGGAATTTCAAGGGAACTCTTTCGAAGGCCCAATACCAGCCAACTTTTCTCAACTGACCTCATTGGAGTATCTGCTAATCAGTGATATATACAACGGGAGCTCTTCTCTTGATTTCATAAAAAATCTGAAAAGCTTGACTGATTTAGGACTACGAAACGCATTAATCACCGGTACCATCCCTTCTGATATTGGAGAATATCAAAGTCTAAAGACATTGGATCTGAGTTTCAACAATTTGACAGGCCAACTACCAAGTTCTTTGTTCAACTTGAGTAATCTTACATACTTGTTTCTTGGAAACAATAGTCTCTCTGGACCTCTTCCGAGCCAAAAGAGCTTTCAACTTCAGACTATAGATTTGTCTTACAATTTTTTATCAGGAAGCCTTCCCCAGTGGGTGACCACAATGTCGCAACTGAACTTAGTGGTCAACAACTTCACATTTGACGGTTCAAACATAGCTCTTCCTCGATTGAATTGCCTCCAGAGAAATTTTCCATGCAATCGAAATACCCCACGATGTAATATGCTCTTATGTCACCCATCATTTACTATTTTAACGCAACGTCATGTTACAAGCTTCTCAATCAACTGTGGTGGACAGCAAATGACGGGAAGTGATGGCATATTGTATGAGAGTGACGACTCAGAACTTGGTCTAGCAACATTCAGTGTAACAGAGAATTGGGCTGTCAGCAATGTCGGTTTGCTTTCTGACCGTACCTCATCGTTGAACAAGACCGTTGCACAAGTCACCGGAACAAATGGGACCCCGGAGCTTCTCCAAACTTCAAGAACGTCCACAGGATCACTGAGATACTATGGCCTGGGCCTTAAGAATGGGCTCTACAATGTAACATTGCACTTTGCAGAGACGGTTTATGAAAGTCGCACTTCACAAACTTGGCAAAGTCTAGGACGACGTGTATTTGATATCTATATTCAGGGTACCCAAAGGACGAAGGACTTTGACATATCGAAGGAGGCAGGTGGTGTTAACCAAGCAGTTGTGAGAAAATTTAATGTTAACGTGTTAGAGAATTATCTTGAAATTCATCTATTCTGGGCTGGTAAAGGGACTTGTTGCATACCCAATTATGGTGATTACGGCCCACTAATAGCAGCTGTCCATGCTGCTTCAGATTTTACAAAGAAGAGCAATATTGGGTTGATAGTTGGTATTGCAGTTCTTGTTGGAGTTGTGAGCTTGCTATTAATATTTGCAATTCTATATATGAGGAGGAAAAAATCAGAAAAAGAAGACGATGAAGATATTCTAGGATTAGGCCCCCGGCCATATACTTTCAGTTATGTTGAATTGAGAACTGCAACTGAAGATTTTAATCCTTCAAATAAGCTAGGAGAGGGAGGATATGGCCCCGTTTACAAGGGTACACTTTCTGATGGTAGAGTAGTGGCTGTGAAGCAACTTTCAGTATCATCTCACCAAGGGAAGAGTCAATTTGTATCTGAAATTGCTACCATATCTGCTGTGCAACATCGGAATCTAGTGAAATTGTATGGATGCTGCATTGAAGGCAGACACCGCATTTTGGTTTATGAGTATCTTGAAAACAAGAGCCTTGATCAGGCACTTTTTGGAACAAGTAACTTGCACCTTGACTGGCCTACTCGATTCAATATATTGTTGGGAACAGCAAGAGGATTTGCTTACCTTCATGAGGAGTCAAGGCCAAGGATTGTACATCGAGATGTCAAAGCGAGTAATATTTTGCTTGATGCAGAACTCTCCCCAAAAATATCAGATTTTGGATTGGCAAAGCTTTATGATGATGAGATAACCCACATCAGCACCCGGGTTGCAGGGACAATAGGCTATTTGGCACCGGAGTATGCATTGTTTGGACATTTGACAGAGAAGGCCGATGTGTTTGGTTTTGGAGTCGTTGTTTTAGAGATCCTCAGCGGGAGACCAAACTCTTATAATAACTTGGATCTAGAAAAGATTTCTCTTCTTGAATGGGTGTGGGCTCTACATGAAAATGACCAAACTCTAGGGCTGGTGGATCCGAGATTGACAAAGTTTGATGAAACTGAAGCAACTAGATTGATAAGAGTAGCTCTCATGTGCACGCAGGGATCACCGATGGCACGACCATCTATGTCACGCGTGGTTGCAATGCTCTCTGGAGACATTGACGTAGGCACTGTCATGTCGAAGCCAAGCTATTTGACAGATTATGATTTCAAAGATGTAACAACATCATTAACAAGTAGATTTTTGGCAGCAATGCAAGTGGTGCTAACACTCCCTGGATTGACCATGCGCCTTCTGTAA

Protein Analysis

920

Amino Acids

101.71

Weight (kDa)

5.85

Isoelectric Point (pI)

32.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 21 - 85 3.3e-06 Leucine-rich repeat region
LRR_14 PF23598 71 - 179 2.5e-08 Leucine-rich repeat region
LRR_14 PF23598 184 - 256 1.8e-08 Leucine-rich repeat region
Malectin PF11721 347 - 530 5.1e-37 Malectin domain
Pkinase PF00069 603 - 866 2.6e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 604 - 868 9.3e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2406
AarI CACCTGC 1 cut(s) 1439
AasI GACNNNNNNGTC 2 cut(s) 172, 868
Acc36I ACCTGC 1 cut(s) 1439
Acc65I GGTACC 2 cut(s) 632, 1409
AccB1I GGYRCC 3 cut(s) 632, 1409, 2305
AccB7I CCANNNNNTGG 1 cut(s) 290
AccII CGCG 1 cut(s) 2592
AccIII TCCGGA 1 cut(s) 269
AciI CCGC 2 cut(s) 2008, 2388
AclWI GGATC 8 cut(s) 262, 687, 1276, 2374, 2424, 2483, 2496, 2569
AcoI YGGCCR 1 cut(s) 1754
AcsI RAATTY 6 cut(s) 71, 348, 464, 952, 1478, 1512
AcuI CTGAAG 4 cut(s) 791, 1584, 1815, 2538
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 5 cut(s) 634, 1183, 1411, 1855, 2166
AfiI CCNNNNNNNGG 6 cut(s) 290, 413, 1559, 2014, 2050, 2143
AflII CTTAAG 2 cut(s) 227, 1295
AflIII ACRYGT 3 cut(s) 1384, 1491, 2590
AgeI ACCGGT 2 cut(s) 125, 629
AjiI CACGTC 1 cut(s) 1385
AjnI CCWGG 2 cut(s) 1287, 2734
AjuI GAANNNNNNNTTGG 2 cut(s) 783, 815
AloI GAACNNNNNNTCC 2 cut(s) 265, 297
Alw21I GWGCWC 2 cut(s) 563, 2555
Alw26I GTCTC 4 cut(s) 773, 1328, 2386, 2607
Alw44I GTGCAC 1 cut(s) 2551
AlwI GGATC 8 cut(s) 262, 687, 1276, 2374, 2424, 2483, 2496, 2569
Ama87I CYCGRG 3 cut(s) 308, 412, 2277
Aor13HI TCCGGA 1 cut(s) 269
ApaLI GTGCAC 1 cut(s) 2551
ApeKI GCWGC 4 cut(s) 1583, 1595, 1989, 2708
ApoI RAATTY 6 cut(s) 71, 348, 464, 952, 1478, 1512
ArsI GACNNNNNNTTYG 2 cut(s) 802, 834
AseI ATTAAT 2 cut(s) 623, 1676
AsiGI ACCGGT 2 cut(s) 125, 629
Asp700I GAANNNNTTC 3 cut(s) 119, 479, 1734
Asp718I GGTACC 2 cut(s) 632, 1409
AspLEI GCGC 1 cut(s) 2751
AspS9I GGNCC 8 cut(s) 128, 488, 776, 1230, 1291, 1571, 1748, 1838
AsuC2I CCSGG 4 cut(s) 1235, 1753, 2278, 2279
AsuII TTCGAA 1 cut(s) 483
AvaI CYCGRG 3 cut(s) 308, 412, 2277
AvaII GGWCC 3 cut(s) 128, 776, 1230
BaeGI GKGCMC 1 cut(s) 2555
BamHI GGATCC 1 cut(s) 2488
BanI GGYRCC 3 cut(s) 632, 1409, 2305
BanII GRGCYC 4 cut(s) 204, 563, 1308, 2455
BarI GAAGNNNNNNTAC 4 cut(s) 477, 509, 625, 657
BbsI GAAGAC 1 cut(s) 1728
Bbv12I GWGCWC 2 cut(s) 563, 2555
BbvCI CCTCAGC 1 cut(s) 2384
BbvI GCAGC 4 cut(s) 1582, 1595, 1976, 2720
BccI CCATC 7 cut(s) 212, 644, 1012, 1076, 1859, 2563, 2587
BceAI ACGGC 1 cut(s) 1585
BcgI CGANNNNNNTGC 2 cut(s) 921, 955
BciT130I CCWGG 2 cut(s) 1289, 2736
BclI TGATCA 1 cut(s) 2047
BcnI CCSGG 4 cut(s) 1235, 1753, 2278, 2279
BcoDI GTCTC 4 cut(s) 773, 1328, 2386, 2607
BfaI CTAG 8 cut(s) 1121, 1376, 1739, 1820, 1970, 2420, 2477, 2526
BfmI CTRYAG 1 cut(s) 813
BfrI CTTAAG 2 cut(s) 227, 1295
BfuAI ACCTGC 1 cut(s) 1439
BisI GCNGC 4 cut(s) 1584, 1596, 1990, 2709
BlsI GCNGC 4 cut(s) 1585, 1597, 1991, 2710
Bme1390I CCNGG 6 cut(s) 1235, 1289, 1753, 2278, 2279, 2736
Bme18I GGWCC 3 cut(s) 128, 776, 1230
BmeT110I CYCGRG 3 cut(s) 308, 412, 2277
BmgBI CACGTC 1 cut(s) 1385
BmgT120I GGNCC 8 cut(s) 128, 488, 776, 1230, 1291, 1571, 1748, 1838
BmiI GGNNCC 9 cut(s) 130, 634, 1231, 1232, 1411, 1750, 1840, 2307, 2490
BmrFI CCNGG 6 cut(s) 1235, 1289, 1753, 2278, 2279, 2736
BmrI ACTGGG 1 cut(s) 847
BmsI GCATC 3 cut(s) 404, 1976, 2191
BmuI ACTGGG 1 cut(s) 847
BpiI GAAGAC 1 cut(s) 1728
BplI GAGNNNNNCTC 4 cut(s) 550, 582, 2529, 2561
BpmI CTGGAG 3 cut(s) 267, 929, 2631
Bpu10I CCTNAGC 1 cut(s) 2384
Bpu14I TTCGAA 1 cut(s) 483
BpuEI CTTGAG 1 cut(s) 751
BpuMI CCSGG 4 cut(s) 1235, 1753, 2278, 2279
BsaBI GATNNNNATC 1 cut(s) 1803
BsaHI GRCGYC 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 2386
BsaJI CCNNGG 8 cut(s) 210, 1233, 1288, 1751, 1909, 2156, 2277, 2734
BsaWI WCCGGW 5 cut(s) 125, 269, 629, 1217, 2308
BsaXI ACNNNNNCTCC 4 cut(s) 265, 295, 2355, 2385
Bsc4I CCNNNNNNNGG 6 cut(s) 290, 413, 1559, 2014, 2050, 2143
Bse118I RCCGGY 3 cut(s) 125, 136, 629
Bse1I ACTGG 2 cut(s) 853, 2090
Bse3DI GCAATG 4 cut(s) 1165, 1319, 2607, 2718
Bse8I GATNNNNATC 1 cut(s) 1803
BseAI TCCGGA 1 cut(s) 269
BseBI CCWGG 2 cut(s) 1289, 2736
BseDI CCNNGG 8 cut(s) 210, 1233, 1288, 1751, 1909, 2156, 2277, 2734
BseGI GGATG 3 cut(s) 204, 636, 1991
BseJI GATNNNNATC 1 cut(s) 1803
BseLI CCNNNNNNNGG 6 cut(s) 290, 413, 1559, 2014, 2050, 2143
BseMI GCAATG 4 cut(s) 1165, 1319, 2607, 2718
BseMII CTCAG 5 cut(s) 189, 674, 1122, 1265, 2398
BseNI ACTGG 2 cut(s) 853, 2090
BseRI GAGGAG 2 cut(s) 1714, 2159
BseSI GKGCMC 1 cut(s) 2555
BseXI GCAGC 4 cut(s) 1582, 1595, 1976, 2720
Bsh1236I CGCG 1 cut(s) 2592
BshNI GGYRCC 3 cut(s) 632, 1409, 2305
BshTI ACCGGT 2 cut(s) 125, 629
BsiHKAI GWGCWC 2 cut(s) 563, 2555
BsiHKCI CYCGRG 3 cut(s) 308, 412, 2277
BsiSI CCGG 9 cut(s) 126, 137, 270, 630, 1218, 1235, 1753, 2278, 2309
BslFI GGGAC 4 cut(s) 114, 1243, 1552, 2302
BslI CCNNNNNNNGG 6 cut(s) 290, 413, 1559, 2014, 2050, 2143
BsmAI GTCTC 4 cut(s) 773, 1328, 2386, 2607
BsmBI CGTCTC 1 cut(s) 1328
BsmFI GGGAC 4 cut(s) 114, 1243, 1552, 2302
BsmI GAATGC 1 cut(s) 140
Bso31I GGTCTC 1 cut(s) 2386
BsoBI CYCGRG 3 cut(s) 308, 412, 2277
Bsp119I TTCGAA 1 cut(s) 483
Bsp1286I GDGCHC 5 cut(s) 204, 563, 1308, 2455, 2555
Bsp13I TCCGGA 1 cut(s) 269
Bsp1407I TGTACA 1 cut(s) 2164
Bsp143I GATC 8 cut(s) 254, 679, 1268, 2047, 2379, 2416, 2488, 2561
BspACI CCGC 2 cut(s) 2008, 2388
BspCNI CTCAG 5 cut(s) 190, 675, 1121, 1266, 2397
BspEI TCCGGA 1 cut(s) 269
BspFNI CGCG 1 cut(s) 2592
BspHI TCATGA 1 cut(s) 2140
BspLI GGNNCC 9 cut(s) 130, 634, 1231, 1232, 1411, 1750, 1840, 2307, 2490
BspMI ACCTGC 1 cut(s) 1439
BspPI GGATC 8 cut(s) 262, 687, 1276, 2374, 2424, 2483, 2496, 2569
BspQI GCTCTTC 3 cut(s) 568, 930, 1610
BspT104I TTCGAA 1 cut(s) 483
BspT107I GGYRCC 3 cut(s) 632, 1409, 2305
BspTI CTTAAG 2 cut(s) 227, 1295
BspTNI GGTCTC 1 cut(s) 2386
BsrDI GCAATG 4 cut(s) 1165, 1319, 2607, 2718
BsrFI RCCGGY 3 cut(s) 125, 136, 629
BsrGI TGTACA 1 cut(s) 2164
BsrI ACTGG 2 cut(s) 853, 2090
BssAI RCCGGY 3 cut(s) 125, 136, 629
BssECI CCNNGG 8 cut(s) 210, 1233, 1288, 1751, 1909, 2156, 2277, 2734
BssMI GATC 8 cut(s) 254, 679, 1268, 2047, 2379, 2416, 2488, 2561
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 3 cut(s) 210, 1909, 2156
Bst2UI CCWGG 2 cut(s) 1289, 2736
Bst4CI ACNGT 6 cut(s) 911, 1055, 1181, 1204, 1339, 2632
Bst6I CTCTTC 6 cut(s) 568, 786, 930, 1610, 1910, 2439
BstACI GRCGYC 1 cut(s) 183
BstAFI CTTAAG 2 cut(s) 227, 1295
BstAPI GCANNNNNTGC 1 cut(s) 2722
BstAUI TGTACA 1 cut(s) 2164
BstBI TTCGAA 1 cut(s) 483
BstC8I GCNNGC 2 cut(s) 1670, 2555
BstDEI CTNAG 6 cut(s) 198, 683, 883, 1108, 1274, 2384
BstEII GGTNACC 1 cut(s) 859
BstENI CCTNNNNNAGG 2 cut(s) 2048, 2141
BstF5I GGATG 3 cut(s) 204, 636, 1991
BstFNI CGCG 1 cut(s) 2592
BstHHI GCGC 1 cut(s) 2751
BstKTI GATC 8 cut(s) 257, 682, 1271, 2050, 2382, 2419, 2491, 2564
BstMAI GTCTC 4 cut(s) 773, 1328, 2386, 2607
BstMBI GATC 8 cut(s) 254, 679, 1268, 2047, 2379, 2416, 2488, 2561
BstMWI GCNNNNNNNGC 5 cut(s) 795, 1592, 1998, 2007, 2722
BstNI CCWGG 2 cut(s) 1289, 2736
BstNSI RCATGY 2 cut(s) 11, 382
BstPI GGTNACC 1 cut(s) 859
BstSCI CCNGG 6 cut(s) 1233, 1287, 1751, 2276, 2277, 2734
BstSFI CTRYAG 1 cut(s) 813
BstSLI GKGCMC 1 cut(s) 2555
BstUI CGCG 1 cut(s) 2592
BstV1I GCAGC 4 cut(s) 1582, 1595, 1976, 2720
BstV2I GAAGAC 1 cut(s) 1728
BstX2I RGATCY 4 cut(s) 679, 2379, 2416, 2488
BstYI RGATCY 4 cut(s) 679, 2379, 2416, 2488
BtrI CACGTC 1 cut(s) 1385
BtsCI GGATG 3 cut(s) 204, 636, 1991
BtsIMutI CAGTG 5 cut(s) 547, 860, 1138, 1271, 2628
BveI ACCTGC 1 cut(s) 1439
Cac8I GCNNGC 2 cut(s) 1670, 2555
CciI TCATGA 1 cut(s) 2140
CfoI GCGC 1 cut(s) 2751
Cfr10I RCCGGY 3 cut(s) 125, 136, 629
Cfr13I GGNCC 8 cut(s) 128, 488, 776, 1230, 1291, 1571, 1748, 1838
Cfr9I CCCGGG 1 cut(s) 2277
CseI GACGC 1 cut(s) 172
Csp6I GTAC 5 cut(s) 633, 1182, 1410, 1854, 2165
CspAI ACCGGT 2 cut(s) 125, 629
CspCI CAANNNNNGTGG 4 cut(s) 1039, 1074, 2700, 2735
CviQI GTAC 5 cut(s) 633, 1182, 1410, 1854, 2165
DdeI CTNAG 6 cut(s) 198, 683, 883, 1108, 1274, 2384
DpnI GATC 8 cut(s) 256, 681, 1270, 2049, 2381, 2418, 2490, 2563
DpnII GATC 8 cut(s) 254, 679, 1268, 2047, 2379, 2416, 2488, 2561
DrdI GACNNNNNNGTC 2 cut(s) 172, 868
DseDI GACNNNNNNGTC 2 cut(s) 172, 868
EaeI YGGCCR 1 cut(s) 1754
Eam1104I CTCTTC 6 cut(s) 568, 786, 930, 1610, 1910, 2439
EarI CTCTTC 6 cut(s) 568, 786, 930, 1610, 1910, 2439
Ecl136II GAGCTC 1 cut(s) 561
Eco130I CCWWGG 3 cut(s) 210, 1909, 2156
Eco24I GRGCYC 4 cut(s) 204, 563, 1308, 2455
Eco31I GGTCTC 1 cut(s) 2386
Eco32I GATATC 2 cut(s) 89, 1396
Eco47I GGWCC 3 cut(s) 128, 776, 1230
Eco53kI GAGCTC 1 cut(s) 561
Eco57I CTGAAG 4 cut(s) 791, 1584, 1815, 2538
Eco88I CYCGRG 3 cut(s) 308, 412, 2277
Eco91I GGTNACC 1 cut(s) 859
EcoICRI GAGCTC 1 cut(s) 561
EcoNI CCTNNNNNAGG 2 cut(s) 2048, 2141
EcoO109I RGGNCCY 2 cut(s) 1230, 1748
EcoO65I GGTNACC 1 cut(s) 859
EcoRII CCWGG 2 cut(s) 1287, 2734
EcoRV GATATC 2 cut(s) 89, 1396
EcoT14I CCWWGG 3 cut(s) 210, 1909, 2156
EcoT22I ATGCAT 1 cut(s) 2320
EcoT38I GRGCYC 4 cut(s) 204, 563, 1308, 2455
ErhI CCWWGG 3 cut(s) 210, 1909, 2156
Esp3I CGTCTC 1 cut(s) 1328
FalI AAGNNNNNCTT 2 cut(s) 1339, 1371
FaqI GGGAC 4 cut(s) 114, 1243, 1552, 2302
FauI CCCGC 1 cut(s) 2381
FbaI TGATCA 1 cut(s) 2047
Fnu4HI GCNGC 4 cut(s) 1584, 1596, 1990, 2709
FokI GGATG 3 cut(s) 191, 623, 1998
FriOI GRGCYC 4 cut(s) 204, 563, 1308, 2455
Fsp4HI GCNGC 4 cut(s) 1584, 1596, 1990, 2709
FspBI CTAG 8 cut(s) 1121, 1376, 1739, 1820, 1970, 2420, 2477, 2526
GlaI GCGC 1 cut(s) 2750
GluI GCNGC 4 cut(s) 1584, 1596, 1990, 2709
GsuI CTGGAG 3 cut(s) 267, 929, 2631
HapII CCGG 9 cut(s) 126, 137, 270, 630, 1218, 1235, 1753, 2278, 2309
HgaI GACGC 1 cut(s) 172
HhaI GCGC 1 cut(s) 2751
Hin1I GRCGYC 1 cut(s) 183
Hin6I GCGC 1 cut(s) 2749
HinP1I GCGC 1 cut(s) 2749
HincII GTYRAC 3 cut(s) 892, 1459, 1489
HindII GTYRAC 3 cut(s) 892, 1459, 1489
HindIII AAGCTT 3 cut(s) 592, 1039, 2243
HinfI GANTC 8 cut(s) 166, 336, 1106, 1918, 1966, 2097, 2147, 2364
HpaI GTTAAC 2 cut(s) 1459, 1489
HpaII CCGG 9 cut(s) 126, 137, 270, 630, 1218, 1235, 1753, 2278, 2309
Hpy166II GTNNAC 9 cut(s) 335, 892, 1061, 1263, 1459, 1489, 1846, 1856, 2553
Hpy8I GTNNAC 9 cut(s) 335, 892, 1061, 1263, 1459, 1489, 1846, 1856, 2553
Hpy99I CGWCG 1 cut(s) 1386
HpyCH4III ACNGT 6 cut(s) 911, 1055, 1181, 1204, 1339, 2632
HpyCH4IV ACGT 5 cut(s) 1027, 1259, 1384, 1491, 2622
HpyF10VI GCNNNNNNNGC 5 cut(s) 795, 1592, 1998, 2007, 2722
HpyF3I CTNAG 6 cut(s) 198, 683, 883, 1108, 1274, 2384
HpySE526I ACGT 5 cut(s) 1027, 1259, 1384, 1491, 2622
Hsp92I GRCGYC 1 cut(s) 183
HspAI GCGC 1 cut(s) 2749
KflI GGGWCCC 1 cut(s) 1230
Kpn2I TCCGGA 1 cut(s) 269
KpnI GGTACC 2 cut(s) 636, 1413
Ksp22I TGATCA 1 cut(s) 2047
KspAI GTTAAC 2 cut(s) 1459, 1489
Kzo9I GATC 8 cut(s) 254, 679, 1268, 2047, 2379, 2416, 2488, 2561
LguI GCTCTTC 3 cut(s) 568, 930, 1610
LmnI GCTCC 4 cut(s) 214, 232, 558, 1237
Lsp1109I GCAGC 4 cut(s) 1582, 1595, 1976, 2720
LweI GCATC 3 cut(s) 404, 1976, 2191
MaeI CTAG 8 cut(s) 1121, 1376, 1739, 1820, 1970, 2420, 2477, 2526
MaeII ACGT 5 cut(s) 1027, 1259, 1384, 1491, 2622
MalI GATC 8 cut(s) 256, 681, 1270, 2049, 2381, 2418, 2490, 2563
MboI GATC 8 cut(s) 254, 679, 1268, 2047, 2379, 2416, 2488, 2561
MfeI CAATTG 2 cut(s) 22, 177
MflI RGATCY 4 cut(s) 679, 2379, 2416, 2488
MhlI GDGCHC 5 cut(s) 204, 563, 1308, 2455, 2555
MluI ACGCGT 1 cut(s) 2590
MlyI GAGTC 6 cut(s) 160, 330, 1100, 1927, 2156, 2373
MmeI TCCRAC 1 cut(s) 1636
Mph1103I ATGCAT 1 cut(s) 2320
MroI TCCGGA 1 cut(s) 269
MroXI GAANNNNTTC 3 cut(s) 119, 479, 1734
MslI CAYNNNNRTG 4 cut(s) 377, 2172, 2463, 2633
MspA1I CMGCKG 3 cut(s) 330, 1586, 2388
MspCI CTTAAG 2 cut(s) 227, 1295
MspI CCGG 9 cut(s) 126, 137, 270, 630, 1218, 1235, 1753, 2278, 2309
MspR9I CCNGG 6 cut(s) 1235, 1289, 1753, 2278, 2279, 2736
MunI CAATTG 2 cut(s) 22, 177
Mva1269I GAATGC 1 cut(s) 140
MvaI CCWGG 2 cut(s) 1289, 2736
MvnI CGCG 1 cut(s) 2592
MwoI GCNNNNNNNGC 5 cut(s) 795, 1592, 1998, 2007, 2722
NciI CCSGG 4 cut(s) 1235, 1753, 2278, 2279
NdeII GATC 8 cut(s) 254, 679, 1268, 2047, 2379, 2416, 2488, 2561
NlaIV GGNNCC 9 cut(s) 130, 634, 1231, 1232, 1411, 1750, 1840, 2307, 2490
NmuCI GTSAC 5 cut(s) 859, 998, 1100, 1213, 2586
NsiI ATGCAT 1 cut(s) 2320
NspI RCATGY 2 cut(s) 11, 382
NspV TTCGAA 1 cut(s) 483
PaeR7I CTCGAG 1 cut(s) 308
PagI TCATGA 1 cut(s) 2140
PaqCI CACCTGC 1 cut(s) 1439
PciSI GCTCTTC 3 cut(s) 568, 930, 1610
PctI GAATGC 1 cut(s) 140
PdmI GAANNNNTTC 3 cut(s) 119, 479, 1734
PfeI GAWTC 2 cut(s) 1966, 2097
PflMI CCANNNNNTGG 1 cut(s) 290
PinAI ACCGGT 2 cut(s) 125, 629
PkrI GCNGC 4 cut(s) 1585, 1597, 1991, 2710
PleI GAGTC 6 cut(s) 160, 330, 1100, 1926, 2155, 2372
PpsI GAGTC 6 cut(s) 160, 330, 1100, 1926, 2155, 2372
PpuMI RGGWCCY 1 cut(s) 1230
PshBI ATTAAT 2 cut(s) 623, 1676
PsiI TTATAA 1 cut(s) 2406
Psp124BI GAGCTC 1 cut(s) 563
Psp5II RGGWCCY 1 cut(s) 1230
Psp6I CCWGG 2 cut(s) 1287, 2734
PspEI GGTNACC 1 cut(s) 859
PspGI CCWGG 2 cut(s) 1287, 2734
PspN4I GGNNCC 9 cut(s) 130, 634, 1231, 1232, 1411, 1750, 1840, 2307, 2490
PspPI GGNCC 8 cut(s) 128, 488, 776, 1230, 1291, 1571, 1748, 1838
PspPPI RGGWCCY 1 cut(s) 1230
PspXI VCTCGAGB 1 cut(s) 308
PsuI RGATCY 4 cut(s) 679, 2379, 2416, 2488
PvuII CAGCTG 2 cut(s) 330, 1586
RsaI GTAC 5 cut(s) 634, 1183, 1411, 1855, 2166
RsaNI GTAC 5 cut(s) 633, 1182, 1410, 1854, 2165
RseI CAYNNNNRTG 4 cut(s) 377, 2172, 2463, 2633
SacI GAGCTC 1 cut(s) 563
SapI GCTCTTC 3 cut(s) 568, 930, 1610
SatI GCNGC 4 cut(s) 1584, 1596, 1990, 2709
Sau3AI GATC 8 cut(s) 254, 679, 1268, 2047, 2379, 2416, 2488, 2561
Sau96I GGNCC 8 cut(s) 128, 488, 776, 1230, 1291, 1571, 1748, 1838
SchI GAGTC 6 cut(s) 160, 330, 1100, 1927, 2156, 2373
ScrFI CCNGG 6 cut(s) 1235, 1289, 1753, 2278, 2279, 2736
SduI GDGCHC 5 cut(s) 204, 563, 1308, 2455, 2555
SfaNI GCATC 3 cut(s) 404, 1976, 2191
SfcI CTRYAG 1 cut(s) 813
Sfr274I CTCGAG 1 cut(s) 308
SfuI TTCGAA 1 cut(s) 483
SinI GGWCC 3 cut(s) 128, 776, 1230
SlaI CTCGAG 1 cut(s) 308
SmaI CCCGGG 1 cut(s) 2279
SmiMI CAYNNNNRTG 4 cut(s) 377, 2172, 2463, 2633
SmlI CTYRAG 4 cut(s) 227, 308, 730, 1295
SmoI CTYRAG 4 cut(s) 227, 308, 730, 1295
SsiI CCGC 2 cut(s) 2008, 2388
SspI AATATT 3 cut(s) 1624, 1680, 2191
SspMI CTAG 8 cut(s) 1121, 1376, 1739, 1820, 1970, 2420, 2477, 2526
SstI GAGCTC 1 cut(s) 563
StyD4I CCNGG 6 cut(s) 1233, 1287, 1751, 2276, 2277, 2734
StyI CCWWGG 3 cut(s) 210, 1909, 2156
TaaI ACNGT 6 cut(s) 911, 1055, 1181, 1204, 1339, 2632
TaiI ACGT 5 cut(s) 1030, 1262, 1387, 1494, 2625
TaqI TCGA 8 cut(s) 309, 483, 931, 967, 1439, 2095, 2170, 2639
TatI WGTACW 1 cut(s) 2164
TfiI GAWTC 2 cut(s) 1966, 2097
TscAI CASTG 5 cut(s) 547, 860, 1138, 1278, 2635
TseFI GTSAC 5 cut(s) 859, 998, 1100, 1213, 2586
TseI GCWGC 4 cut(s) 1583, 1595, 1989, 2708
Tsp45I GTSAC 5 cut(s) 859, 998, 1100, 1213, 2586
TspMI CCCGGG 1 cut(s) 2277
TspRI CASTG 5 cut(s) 547, 860, 1138, 1278, 2635
Van91I CCANNNNNTGG 1 cut(s) 290
Vha464I CTTAAG 2 cut(s) 227, 1295
VneI GTGCAC 1 cut(s) 2551
VpaK11BI GGWCC 3 cut(s) 128, 776, 1230
VspI ATTAAT 2 cut(s) 623, 1676
XagI CCTNNNNNAGG 2 cut(s) 2048, 2141
XapI RAATTY 6 cut(s) 71, 348, 464, 952, 1478, 1512
XbaI TCTAGA 1 cut(s) 2419
XceI RCATGY 2 cut(s) 11, 382
XhoI CTCGAG 1 cut(s) 308
XmaI CCCGGG 1 cut(s) 2277
XmnI GAANNNNTTC 3 cut(s) 119, 479, 1734
XspI CTAG 8 cut(s) 1121, 1376, 1739, 1820, 1970, 2420, 2477, 2526
Zsp2I ATGCAT 1 cut(s) 2320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.