MD05G1260900.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000088
Physical Location & Seq
Reverse (-)
39607089 .. 39610394
3306 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1260900.v1.1.491

Sequence Viewer

Length: 1908 bp
ATGCAGGAACTTAGTGATGCAAACTTCTCAATCAACTGTGGTGGACCGGAAATGAAGGGACGTGATGGCATATCGTATGAGGCCGAAGACTCAGCTCTTGGTCCAGCAACATTCAATGTAACCAGTACAGAGAAATGGGCTGTCAGCAATGTCGGTATGTTTTCTGACGGAGTGAACCGATCGTTTCTGGAAAATACCCTTGCACAAGTCACCGGAACAGATGTGACCCCAGAGCTTTTCCAGACTTCAAGATTGTCCCCAGGATCACTGAGATACTATGGCCTGGGTCTTGAGAATGGACCTTACACTGTAACATTGCACTTTGCAGAGACGGTTTTTGATAGGCGCATTCAGCAAACTTGGGAAAGTCTAGGACGGCGTGTATTTGACATCTATATTCAGGGTATCTGCAGGATGCAGGACTTTGACATATCCAAGGAGGCAGGTGGGGTTAACCTAGGAATTATGAAAAGATTCAATGTTAGCGTGTCAGAGAATTATCTTGAAATTCATCTGTTCTGGGCTGGTAAAGGGACTTGTTGCATACCCAATCAAGGTGATTACGGCTCACTAATAGCAGCTGTCCATGCTGCTTCAGATTTTACACCAACAGTTTCCGGGCTTCCACCAACTACCCCAGGAAAGAAGAGCAGGACTGGGTTGATAGTTGGTATTGCAGTTCCTGTTGGAGTTGTGAGCTTGCTATTAATATTTGCGATTCTATATATGAGGAGGAAAAAATCAGAAAAAGAGGATGACGAAGATATTCTAGGATTAGGCCCTCGACCAAATACTTTTAGTTATTCTGAGTTGAGAGTTGCAACCGAAGATTTTAATCCTTCAAATAAGTTAGGAGAAGGAGGATATGGCCCTGTTTACAAGGGTACACTTTCTGATGGGAGAGTAGTGGCTGTGAAGCAACTTTCAGTAGCATCTCACCAAGGGAAGAGTCAATTTGTATCAGAAATTTCTACCATATCTGCTGTGCAACATCGGAATCTAGTGAAATTGTATGGATGCTGCATTGAAGGCAGCCAACGCATTTTGGTTTATGAGTATCTTGAAAACAAGAGCCTTGATCAAGCACTTTTTGGAACAAGTAACTTGCACCTTGACTGGCCGACTCGATTCAATATATTGTTGGGAACAGCAAGAGGACTTGCTTACCTTCATGAGGAGTCAAGGCCAAGGATTGTACATCGCGATGTCAAAGCGAGTAATATTTTGCTCGATGCAGAACTCTCCCAAAAAATATCAGATTTTGGACTGGCAAAGCTTTATGATGACGAGAAAACCCACATCAGCACCCGGGTTGCAGGGACAATAGGCTATTTGGCACCGGAGTATGCATTGTTTGGACATTTGACAGAGAAGGCCGATGTGTTTGGTTTTGGAGTCGTTGTTTTGGAGATCCTTAGCGGGAGACCAAACTCTTACAATAACTTGGATCCAAAAAAGATTTATCTTCTTGAATGGGTATGGACTCTACATGAAAATGACCAAACTCTGGGGCTGGTGGATCCAAGATTGACAGAGTTTGATGAAACTGAAGCAACTAGATTGATAAGAGCAGCTCTCCTGTGCACGCAGGGATCACCGATGGCAAGGCCATCTATGTCACGCGTGGTTGCAATGCTCTCTGGAGACATTGACATAGGCACTATCATGTTGAAGCCAAGCTATTTGACAGATTATGATTTTAAAGATGTAACAACATTGCCAACAGGTAGATTTTTGGTGGAGGATGATACCCCATCAACTGCATCCAGGGATAGTAATGTTCGCCTCAACTATCAGCCAGGAGGCAGCAATGCAAGTGGTGCTAACACTTCCTGGATTGACCCTGCATCTTCTCCTGTAAACGTCACTCAATCACTGCTCGCTGGCATTAGAGGAAAAGGAAGGTGA

Protein Analysis

636

Amino Acids

69.66

Weight (kDa)

5.53

Isoelectric Point (pI)

32.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 9 - 194 1.2e-36 Malectin domain
Pkinase PF00069 280 - 542 4.2e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 281 - 543 5.2e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 434
Acc36I ACCTGC 1 cut(s) 434
AccB1I GGYRCC 1 cut(s) 1336
AccB7I CCANNNNNTGG 1 cut(s) 1507
AccII CGCG 2 cut(s) 1203, 1623
AciI CCGC 1 cut(s) 1419
AclWI GGATC 7 cut(s) 271, 1405, 1442, 1455, 1514, 1527, 1600
AcoI YGGCCR 1 cut(s) 1118
AcsI RAATTY 2 cut(s) 507, 966
AcuI CTGAAG 2 cut(s) 579, 1569
AfaI GTAC 3 cut(s) 127, 886, 1197
AfiI CCNNNNNNNGG 3 cut(s) 554, 1174, 1507
AflIII ACRYGT 1 cut(s) 1621
AjiI CACGTC 1 cut(s) 62
AjnI CCWGG 6 cut(s) 259, 282, 637, 1766, 1798, 1832
AluBI AGCT 7 cut(s) 95, 235, 581, 699, 1276, 1574, 1680
AluI AGCT 7 cut(s) 95, 235, 581, 699, 1276, 1574, 1680
Alw21I GWGCWC 1 cut(s) 1586
Alw26I GTCTC 3 cut(s) 323, 1417, 1638
Alw44I GTGCAC 1 cut(s) 1582
AlwI GGATC 7 cut(s) 271, 1405, 1442, 1455, 1514, 1527, 1600
AlwNI CAGNNNCTG 1 cut(s) 683
Ama87I CYCGRG 1 cut(s) 1308
AoxI GGCC 8 cut(s) 81, 280, 778, 868, 1118, 1184, 1374, 1607
ApaLI GTGCAC 1 cut(s) 1582
ApeKI GCWGC 6 cut(s) 578, 590, 1020, 1032, 1571, 1806
ApoI RAATTY 2 cut(s) 507, 966
AseI ATTAAT 1 cut(s) 707
Asp700I GAANNNNTTC 2 cut(s) 473, 765
AspA2I CCTAGG 1 cut(s) 457
AspLEI GCGC 1 cut(s) 348
AspS9I GGNCC 5 cut(s) 44, 101, 299, 779, 869
AsuC2I CCSGG 3 cut(s) 619, 1309, 1310
AsuHPI GGTGA 4 cut(s) 202, 569, 929, 1587
AvaI CYCGRG 1 cut(s) 1308
AvaII GGWCC 3 cut(s) 44, 101, 299
AvrII CCTAGG 1 cut(s) 457
BaeGI GKGCMC 1 cut(s) 1586
BamHI GGATCC 2 cut(s) 1447, 1519
BanI GGYRCC 1 cut(s) 1336
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 1 cut(s) 1586
BbvI GCAGC 6 cut(s) 577, 590, 1007, 1044, 1583, 1818
BccI CCATC 5 cut(s) 59, 890, 1594, 1618, 1762
BceAI ACGGC 2 cut(s) 392, 580
BciT130I CCWGG 6 cut(s) 261, 284, 639, 1768, 1800, 1834
BclI TGATCA 1 cut(s) 1078
BcnI CCSGG 3 cut(s) 619, 1309, 1310
BcoDI GTCTC 3 cut(s) 323, 1417, 1638
BfaI CTAG 5 cut(s) 371, 458, 770, 1001, 1557
BfmI CTRYAG 1 cut(s) 409
BfuAI ACCTGC 1 cut(s) 434
BisI GCNGC 6 cut(s) 579, 591, 1021, 1033, 1572, 1807
BlnI CCTAGG 1 cut(s) 457
BlsI GCNGC 6 cut(s) 580, 592, 1022, 1034, 1573, 1808
Bme1390I CCNGG 9 cut(s) 261, 284, 619, 639, 1309, 1310, 1768, 1800, 1834
Bme18I GGWCC 3 cut(s) 44, 101, 299
BmeT110I CYCGRG 1 cut(s) 1308
BmgBI CACGTC 1 cut(s) 62
BmgT120I GGNCC 5 cut(s) 44, 101, 299, 779, 869
BmiI GGNNCC 3 cut(s) 1338, 1449, 1521
BmrFI CCNGG 9 cut(s) 261, 284, 619, 639, 1309, 1310, 1768, 1800, 1834
BmrI ACTGGG 1 cut(s) 666
BmsI GCATC 7 cut(s) 7, 405, 941, 1007, 1222, 1772, 1856
BmuI ACTGGG 1 cut(s) 666
BpiI GAAGAC 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 1560, 1592
BpmI CTGGAG 1 cut(s) 1662
Bpu10I CCTNAGC 1 cut(s) 1415
BpuEI CTTGAG 1 cut(s) 311
BpuMI CCSGG 3 cut(s) 619, 1309, 1310
BsaBI GATNNNNATC 1 cut(s) 834
BsaI GGTCTC 1 cut(s) 1417
BsaJI CCNNGG 9 cut(s) 259, 283, 435, 457, 637, 940, 1187, 1308, 1767
BsaWI WCCGGW 3 cut(s) 46, 212, 1339
BsaXI ACNNNNNCTCC 2 cut(s) 1386, 1416
Bsc4I CCNNNNNNNGG 3 cut(s) 554, 1174, 1507
Bse1I ACTGG 4 cut(s) 123, 661, 1121, 1272
Bse3DI GCAATG 5 cut(s) 154, 314, 1638, 1715, 1816
Bse8I GATNNNNATC 1 cut(s) 834
BseBI CCWGG 6 cut(s) 261, 284, 639, 1768, 1800, 1834
BseDI CCNNGG 9 cut(s) 259, 283, 435, 457, 637, 940, 1187, 1308, 1767
BseGI GGATG 5 cut(s) 420, 760, 1022, 1750, 1763
BseJI GATNNNNATC 1 cut(s) 834
BseLI CCNNNNNNNGG 3 cut(s) 554, 1174, 1507
BseMI GCAATG 5 cut(s) 154, 314, 1638, 1715, 1816
BseMII CTCAG 3 cut(s) 105, 260, 798
BseNI ACTGG 4 cut(s) 123, 661, 1121, 1272
BseRI GAGGAG 2 cut(s) 745, 1190
BseSI GKGCMC 1 cut(s) 1586
BseXI GCAGC 6 cut(s) 577, 590, 1007, 1044, 1583, 1818
Bsh1236I CGCG 2 cut(s) 1203, 1623
Bsh1285I CGRYCG 1 cut(s) 182
BshFI GGCC 8 cut(s) 83, 282, 780, 870, 1120, 1186, 1376, 1609
BshNI GGYRCC 1 cut(s) 1336
BsiEI CGRYCG 1 cut(s) 182
BsiHKAI GWGCWC 1 cut(s) 1586
BsiHKCI CYCGRG 1 cut(s) 1308
BsiSI CCGG 5 cut(s) 47, 213, 618, 1309, 1340
BslFI GGGAC 4 cut(s) 72, 241, 547, 1333
BslI CCNNNNNNNGG 3 cut(s) 554, 1174, 1507
BsmAI GTCTC 3 cut(s) 323, 1417, 1638
BsmBI CGTCTC 1 cut(s) 323
BsmFI GGGAC 4 cut(s) 72, 241, 547, 1333
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 8 cut(s) 83, 282, 780, 870, 1120, 1186, 1376, 1609
Bso31I GGTCTC 1 cut(s) 1417
BsoBI CYCGRG 1 cut(s) 1308
Bsp1286I GDGCHC 1 cut(s) 1586
Bsp1407I TGTACA 1 cut(s) 1195
Bsp143I GATC 7 cut(s) 179, 263, 1078, 1410, 1447, 1519, 1592
Bsp68I TCGCGA 1 cut(s) 1203
BspACI CCGC 1 cut(s) 1419
BspANI GGCC 8 cut(s) 83, 282, 780, 870, 1120, 1186, 1376, 1609
BspCNI CTCAG 3 cut(s) 104, 261, 799
BspFNI CGCG 2 cut(s) 1203, 1623
BspHI TCATGA 1 cut(s) 1171
BspLI GGNNCC 3 cut(s) 1338, 1449, 1521
BspMAI CTGCAG 1 cut(s) 413
BspMI ACCTGC 1 cut(s) 434
BspPI GGATC 7 cut(s) 271, 1405, 1442, 1455, 1514, 1527, 1600
BspQI GCTCTTC 1 cut(s) 641
BspT107I GGYRCC 1 cut(s) 1336
BspTNI GGTCTC 1 cut(s) 1417
BsrDI GCAATG 5 cut(s) 154, 314, 1638, 1715, 1816
BsrGI TGTACA 1 cut(s) 1195
BsrI ACTGG 4 cut(s) 123, 661, 1121, 1272
BssECI CCNNGG 9 cut(s) 259, 283, 435, 457, 637, 940, 1187, 1308, 1767
BssMI GATC 7 cut(s) 179, 263, 1078, 1410, 1447, 1519, 1592
BssT1I CCWWGG 4 cut(s) 435, 457, 940, 1187
Bst2UI CCWGG 6 cut(s) 261, 284, 639, 1768, 1800, 1834
Bst4CI ACNGT 4 cut(s) 38, 310, 334, 613
Bst6I CTCTTC 2 cut(s) 641, 941
BstAPI GCANNNNNTGC 1 cut(s) 1820
BstAUI TGTACA 1 cut(s) 1195
BstC8I GCNNGC 4 cut(s) 701, 1586, 1881, 1885
BstDEI CTNAG 5 cut(s) 11, 91, 269, 807, 1415
BstENI CCTNNNNNAGG 1 cut(s) 1172
BstF5I GGATG 5 cut(s) 420, 760, 1022, 1750, 1763
BstFNI CGCG 2 cut(s) 1203, 1623
BstHHI GCGC 1 cut(s) 348
BstKTI GATC 7 cut(s) 182, 266, 1081, 1413, 1450, 1522, 1595
BstMAI GTCTC 3 cut(s) 323, 1417, 1638
BstMBI GATC 7 cut(s) 179, 263, 1078, 1410, 1447, 1519, 1592
BstMCI CGRYCG 1 cut(s) 182
BstMWI GCNNNNNNNGC 5 cut(s) 352, 587, 1029, 1038, 1820
BstNI CCWGG 6 cut(s) 261, 284, 639, 1768, 1800, 1834
BstSCI CCNGG 9 cut(s) 259, 282, 617, 637, 1307, 1308, 1766, 1798, 1832
BstSFI CTRYAG 1 cut(s) 409
BstSLI GKGCMC 1 cut(s) 1586
BstUI CGCG 2 cut(s) 1203, 1623
BstV1I GCAGC 6 cut(s) 577, 590, 1007, 1044, 1583, 1818
BstV2I GAAGAC 1 cut(s) 93
BstX2I RGATCY 3 cut(s) 1410, 1447, 1519
BstYI RGATCY 3 cut(s) 1410, 1447, 1519
BsuRI GGCC 8 cut(s) 83, 282, 780, 870, 1120, 1186, 1376, 1609
BtgZI GCGATG 2 cut(s) 1184, 1218
BtrI CACGTC 1 cut(s) 62
BtsCI GGATG 5 cut(s) 420, 760, 1022, 1750, 1763
BtsI GCAGTG 1 cut(s) 1874
BtsIMutI CAGTG 3 cut(s) 266, 306, 1874
BtuMI TCGCGA 1 cut(s) 1203
BveI ACCTGC 1 cut(s) 434
Cac8I GCNNGC 4 cut(s) 701, 1586, 1881, 1885
CaiI CAGNNNCTG 1 cut(s) 683
CciI TCATGA 1 cut(s) 1171
CfoI GCGC 1 cut(s) 348
Cfr13I GGNCC 5 cut(s) 44, 101, 299, 779, 869
Cfr9I CCCGGG 1 cut(s) 1308
Csp6I GTAC 3 cut(s) 126, 885, 1196
CspCI CAANNNNNGTGG 4 cut(s) 22, 57, 1798, 1833
CviAII CATG 4 cut(s) 587, 1172, 1490, 1666
CviQI GTAC 3 cut(s) 126, 885, 1196
DdeI CTNAG 5 cut(s) 11, 91, 269, 807, 1415
DpnI GATC 7 cut(s) 181, 265, 1080, 1412, 1449, 1521, 1594
DpnII GATC 7 cut(s) 179, 263, 1078, 1410, 1447, 1519, 1592
DraI TTTAAA 1 cut(s) 1702
EaeI YGGCCR 1 cut(s) 1118
Eam1104I CTCTTC 2 cut(s) 641, 941
EarI CTCTTC 2 cut(s) 641, 941
Eco130I CCWWGG 4 cut(s) 435, 457, 940, 1187
Eco31I GGTCTC 1 cut(s) 1417
Eco47I GGWCC 3 cut(s) 44, 101, 299
Eco57I CTGAAG 2 cut(s) 579, 1569
Eco88I CYCGRG 1 cut(s) 1308
EcoNI CCTNNNNNAGG 1 cut(s) 1172
EcoO109I RGGNCCY 1 cut(s) 779
EcoRII CCWGG 6 cut(s) 259, 282, 637, 1766, 1798, 1832
EcoT14I CCWWGG 4 cut(s) 435, 457, 940, 1187
EcoT22I ATGCAT 1 cut(s) 1351
ErhI CCWWGG 4 cut(s) 435, 457, 940, 1187
Esp3I CGTCTC 1 cut(s) 323
FaeI CATG 4 cut(s) 590, 1175, 1493, 1669
FaqI GGGAC 4 cut(s) 72, 241, 547, 1333
FatI CATG 4 cut(s) 586, 1171, 1489, 1665
FauI CCCGC 1 cut(s) 1412
FbaI TGATCA 1 cut(s) 1078
Fnu4HI GCNGC 6 cut(s) 579, 591, 1021, 1033, 1572, 1807
FokI GGATG 5 cut(s) 427, 767, 1029, 1750, 1757
Fsp4HI GCNGC 6 cut(s) 579, 591, 1021, 1033, 1572, 1807
FspBI CTAG 5 cut(s) 371, 458, 770, 1001, 1557
GlaI GCGC 1 cut(s) 347
GluI GCNGC 6 cut(s) 579, 591, 1021, 1033, 1572, 1807
GsuI CTGGAG 1 cut(s) 1662
HaeIII GGCC 8 cut(s) 83, 282, 780, 870, 1120, 1186, 1376, 1609
HapII CCGG 5 cut(s) 47, 213, 618, 1309, 1340
HhaI GCGC 1 cut(s) 348
Hin1II CATG 4 cut(s) 590, 1175, 1493, 1669
Hin6I GCGC 1 cut(s) 346
HinP1I GCGC 1 cut(s) 346
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HindIII AAGCTT 1 cut(s) 1274
HpaI GTTAAC 1 cut(s) 454
HpaII CCGG 5 cut(s) 47, 213, 618, 1309, 1340
HphI GGTGA 4 cut(s) 202, 569, 929, 1587
Hpy166II GTNNAC 7 cut(s) 44, 175, 454, 877, 887, 1584, 1861
Hpy188I TCNGA 9 cut(s) 166, 493, 598, 745, 808, 895, 964, 996, 1258
Hpy8I GTNNAC 7 cut(s) 44, 175, 454, 877, 887, 1584, 1861
HpyAV CCTTC 7 cut(s) 49, 849, 851, 1022, 1178, 1366, 1896
HpyCH4III ACNGT 4 cut(s) 38, 310, 334, 613
HpyCH4IV ACGT 2 cut(s) 61, 1863
HpyF10VI GCNNNNNNNGC 5 cut(s) 352, 587, 1029, 1038, 1820
HpyF3I CTNAG 5 cut(s) 11, 91, 269, 807, 1415
HpySE526I ACGT 2 cut(s) 61, 1863
Hsp92II CATG 4 cut(s) 590, 1175, 1493, 1669
HspAI GCGC 1 cut(s) 346
Ksp22I TGATCA 1 cut(s) 1078
KspAI GTTAAC 1 cut(s) 454
Kzo9I GATC 7 cut(s) 179, 263, 1078, 1410, 1447, 1519, 1592
LguI GCTCTTC 1 cut(s) 641
Lsp1109I GCAGC 6 cut(s) 577, 590, 1007, 1044, 1583, 1818
LweI GCATC 7 cut(s) 7, 405, 941, 1007, 1222, 1772, 1856
MaeI CTAG 5 cut(s) 371, 458, 770, 1001, 1557
MaeII ACGT 2 cut(s) 61, 1863
MaeIII GTNAC 8 cut(s) 118, 208, 223, 310, 1100, 1617, 1708, 1864
MalI GATC 7 cut(s) 181, 265, 1080, 1412, 1449, 1521, 1594
MboI GATC 7 cut(s) 179, 263, 1078, 1410, 1447, 1519, 1592
MboII GAAGA 7 cut(s) 98, 658, 773, 839, 958, 1457, 1842
MflI RGATCY 3 cut(s) 1410, 1447, 1519
MhlI GDGCHC 1 cut(s) 1586
MluCI AATT 6 cut(s) 462, 496, 507, 953, 966, 1007
MluI ACGCGT 1 cut(s) 1621
MlyI GAGTC 6 cut(s) 83, 958, 1117, 1187, 1404, 1477
MmeI TCCRAC 1 cut(s) 667
Mph1103I ATGCAT 1 cut(s) 1351
MroXI GAANNNNTTC 2 cut(s) 473, 765
MseI TTAA 4 cut(s) 453, 707, 834, 1701
MslI CAYNNNNRTG 3 cut(s) 1203, 1494, 1664
MspA1I CMGCKG 1 cut(s) 581
MspI CCGG 5 cut(s) 47, 213, 618, 1309, 1340
MspR9I CCNGG 9 cut(s) 261, 284, 619, 639, 1309, 1310, 1768, 1800, 1834
Mva1269I GAATGC 1 cut(s) 348
MvaI CCWGG 6 cut(s) 261, 284, 639, 1768, 1800, 1834
MvnI CGCG 2 cut(s) 1203, 1623
MwoI GCNNNNNNNGC 5 cut(s) 352, 587, 1029, 1038, 1820
NciI CCSGG 3 cut(s) 619, 1309, 1310
NdeII GATC 7 cut(s) 179, 263, 1078, 1410, 1447, 1519, 1592
NlaIII CATG 4 cut(s) 590, 1175, 1493, 1669
NlaIV GGNNCC 3 cut(s) 1338, 1449, 1521
NmuCI GTSAC 4 cut(s) 208, 223, 1617, 1864
NruI TCGCGA 1 cut(s) 1203
NsiI ATGCAT 1 cut(s) 1351
PagI TCATGA 1 cut(s) 1171
PaqCI CACCTGC 1 cut(s) 434
PciSI GCTCTTC 1 cut(s) 641
PctI GAATGC 1 cut(s) 348
PdmI GAANNNNTTC 2 cut(s) 473, 765
PfeI GAWTC 4 cut(s) 474, 718, 997, 1128
PflMI CCANNNNNTGG 1 cut(s) 1507
PfoI TCCNGGA 1 cut(s) 1832
PkrI GCNGC 6 cut(s) 580, 592, 1022, 1034, 1573, 1808
Ple19I CGATCG 1 cut(s) 182
PleI GAGTC 6 cut(s) 83, 957, 1117, 1186, 1403, 1477
PpsI GAGTC 6 cut(s) 83, 957, 1117, 1186, 1403, 1477
PshBI ATTAAT 1 cut(s) 707
Psp6I CCWGG 6 cut(s) 259, 282, 637, 1766, 1798, 1832
PspGI CCWGG 6 cut(s) 259, 282, 637, 1766, 1798, 1832
PspN4I GGNNCC 3 cut(s) 1338, 1449, 1521
PspPI GGNCC 5 cut(s) 44, 101, 299, 779, 869
PstI CTGCAG 1 cut(s) 413
PstNI CAGNNNCTG 1 cut(s) 683
PsuI RGATCY 3 cut(s) 1410, 1447, 1519
PvuI CGATCG 1 cut(s) 182
PvuII CAGCTG 1 cut(s) 581
RruI TCGCGA 1 cut(s) 1203
RsaI GTAC 3 cut(s) 127, 886, 1197
RsaNI GTAC 3 cut(s) 126, 885, 1196
RseI CAYNNNNRTG 3 cut(s) 1203, 1494, 1664
SapI GCTCTTC 1 cut(s) 641
SaqAI TTAA 4 cut(s) 453, 707, 834, 1701
SatI GCNGC 6 cut(s) 579, 591, 1021, 1033, 1572, 1807
Sau3AI GATC 7 cut(s) 179, 263, 1078, 1410, 1447, 1519, 1592
Sau96I GGNCC 5 cut(s) 44, 101, 299, 779, 869
SchI GAGTC 6 cut(s) 83, 958, 1117, 1187, 1404, 1477
ScrFI CCNGG 9 cut(s) 261, 284, 619, 639, 1309, 1310, 1768, 1800, 1834
SduI GDGCHC 1 cut(s) 1586
SfaNI GCATC 7 cut(s) 7, 405, 941, 1007, 1222, 1772, 1856
SfcI CTRYAG 1 cut(s) 409
SinI GGWCC 3 cut(s) 44, 101, 299
SmaI CCCGGG 1 cut(s) 1310
SmiMI CAYNNNNRTG 3 cut(s) 1203, 1494, 1664
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 6 cut(s) 462, 496, 507, 953, 966, 1007
SsiI CCGC 1 cut(s) 1419
SspI AATATT 2 cut(s) 711, 1222
SspMI CTAG 5 cut(s) 371, 458, 770, 1001, 1557
StyD4I CCNGG 9 cut(s) 259, 282, 617, 637, 1307, 1308, 1766, 1798, 1832
StyI CCWWGG 4 cut(s) 435, 457, 940, 1187
TaaI ACNGT 4 cut(s) 38, 310, 334, 613
TaiI ACGT 2 cut(s) 64, 1866
TaqI TCGA 3 cut(s) 784, 1126, 1230
TasI AATT 6 cut(s) 462, 496, 507, 953, 966, 1007
TatI WGTACW 2 cut(s) 125, 1195
TfiI GAWTC 4 cut(s) 474, 718, 997, 1128
Tru1I TTAA 4 cut(s) 453, 707, 834, 1701
Tru9I TTAA 4 cut(s) 453, 707, 834, 1701
TscAI CASTG 3 cut(s) 273, 313, 1881
TseFI GTSAC 4 cut(s) 208, 223, 1617, 1864
TseI GCWGC 6 cut(s) 578, 590, 1020, 1032, 1571, 1806
Tsp45I GTSAC 4 cut(s) 208, 223, 1617, 1864
TspDTI ATGAA 6 cut(s) 68, 482, 500, 1160, 1506, 1557
TspGWI ACGGA 1 cut(s) 183
TspMI CCCGGG 1 cut(s) 1308
TspRI CASTG 3 cut(s) 273, 313, 1881
Van91I CCANNNNNTGG 1 cut(s) 1507
VneI GTGCAC 1 cut(s) 1582
VpaK11BI GGWCC 3 cut(s) 44, 101, 299
VspI ATTAAT 1 cut(s) 707
XagI CCTNNNNNAGG 1 cut(s) 1172
XapI RAATTY 2 cut(s) 507, 966
XmaI CCCGGG 1 cut(s) 1308
XmaJI CCTAGG 1 cut(s) 457
XmnI GAANNNNTTC 2 cut(s) 473, 765
XspI CTAG 5 cut(s) 371, 458, 770, 1001, 1557
Zsp2I ATGCAT 1 cut(s) 1351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.