Rroxscaffold_1G00007610
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000088
Physical Location & Seq
Reverse (-)
9729425 .. 9740093
10669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00007610.1

Sequence Viewer

Length: 3450 bp
ATGTCACTGATGGTGTGGTTTTGGTATGCTGCAGCAGCCTGCAGCATATTCATCTCTGGGTTTGTTGCACAGGCTCAACCCACCACAGATCCCTCTGAAGTGAGAGCGCTGAATGCAATCTTTCAGAAATGGAATATTACGGCCAATACAGCTGGTGATCCATGCAGTGATCTTGCGGTAGATGAAGATGAAGAGACCGCTTATTTCCATGATGATGATTACAATCTCCTCGTCAAATGCAACTGCTCTTATGATTCAAACTCAACTTGCCACATTACCCAACTGAAAGCAATCACTTTGGACATTGTTGCTCCAATTCCAGAAGAGCTCTATGCTTTGACTTTCCTTCAATATCTGGACTTGGGACACAACGACTTATCAGGGGAACTACCAAAGGAAATTGAAAAACTCACCACTTTGACATTCTTGGGTGTTGGGACACTCAACCTTTCTGGTTCTCTGCCTCCTGAGATGGGGTATTTAAAAACATTAAAAGAGATTTACATTGATAATTCTGGACTTAGCGGTGAGATTCCACCAACATTTGCTAATCTAACAAACTTGGAAATATTTTGGGCAACAGATATAAACCTCACTGGCAGGATACCGGACTTCGTAGGAGATTGGACAAAGATTACTAAATTGTTGACTCTTTTGACAGAGCTGCGAATTACTGATTTAACGATTGCCAATGACAACTCTTCTCTTGGATTTATCAACAATATGAAGTCTCTACAAGTCTTAATGCTGAGGAATAACAATATTTCTGATTCAATTCCTTCCAACATTGGAGATTACCTAAAATTGGTTCACCTGGATTTGAGCTTCAACAATTTGAATGGACAAATACCAGCCTCACTATTTGATATGAATGAGCTTGCCATTCTGTTTCTTGGAAACAATAAGTTGAATGGTAGCCTTCCTGAGTCTAAAAGCTCGTCTCTTCTCGTTGTTAACTTGGTGGCCAACAACTTCAGCATAGACAGTTCAGACAGCAGCAGGGATAGGCAGTTGGTGGAGGTCTTGACGATAGGGAGGGCATTCAAGACCCTGGGGACCAGTGGTGGAGAGTATTGGAAGAAGATTAGAGGATCCAGTGTGGTAAGGAGGTTGGAAGGGATAGTGGGAAGAGGTGGCAAAAAGCAAGAGAGGTTATTTAGTTCAGGTGACTCAAGTCAAGGACAAAAAGGAGGTTCTGGGGTTGGAGACTTGGTTGGAGCCCATGGATGGATATTCCTTAAACAGAGTGCTTCTTTGGCTTCTGTTGTTTGTCTTGGGGTTGGAAAATGGGGAAAAAGGATGAGGTTTTTGAAGAGCATTGGGGTTGTATGGATGGAAACGATTATGAGGGTTTTGGATATTGTTAAGGGTGGGAGTGGTCTACCTTCAGGGTTGAATTGCCTTCAAAGGGGCTTCCCTTGCCACCGTGATAGTCCAGTTTACTATAACTTCATGATTAACTGCGGTGGTCTTCAGGCTACGATATCCAATGGGACTGTGTATGAGAAGGACATTGAGCCCCTTGGTGCAGCTACATATTATGTGACTGACACAAACAGATGGGGAGTTAGCAATGTCGGTCTTAAAACACAGTTTGTAGCTGCATCATATTTGACTGGGACCAACATTCCAAAGTATACAATATCCACTTCATCTCCAATCAGAAATACTTCAGACCCGACGCTATTTCAGAGTGCAAGGATCTCTGCTTCATCACTCAGATACTATGGCTTGGGTCTTGAGAATGGAAACTATACCGTGAAGCTTGAATTTGCAGAACAAGCTATCCTAGATACCCTTAAAAGAAAAAGTCTTGGAAGACGTGTGTTTGATATATATATCCAGGATGTTCTTGTTGTTAAAGATTTTGATATACGGAAGGAGACAAATAAGACATCTTTGCTAGCTATTGAAAAGGTATACAAGGCTCAGGTGGTTTCAGAGAATTACCTTGAAATCCATTTCTTCTGGGCTGGAAAGGGGACTTGCTGTATACCAGAGGAGGGTACTTATGGACCTCTTGTTTCAGCCATCAGTGCTACACCTGGTAAAGTAAATGAAAATTATAATCGGACCAGTTTATTAGTTTACGATGTTTTCTTTCACACTGTGCTCCAGGCTGTTGACCAAAGAGCTTTAATTTCTACAGAATTCAAACCTACCGTCAGCAACAAGCTGCCAAGTAGTAAGAAGAATAGGACTGGGCTTATTGTGGGAATTGTTGTTGGTTGTGGAGTTTTAATTCTGGTGGTGCTGCTTTTATATGTTGTTCAAGGAAGAAAGAGGCACAACACTGATGATGACTATGATGAAGAGCTGTTGGGAATAGATCTTGGACCTCTCACTTTCAGTTTTTCTGAACTGAAGGCAGCTACAAATGACTTTAGTCCAGATAATAAGCTTGGAGAGGGAGGATTTGGGCCTGTCTTCAAAGGTACTCTAAATGATGGAAGAGTAATTGCCGTAAAACAATTGGCTGCATCATCCCACCAAGGAAAGAACCAGTTTGTGACTGAGATTGCCACTATATCTTCTGTTCGACACAATAACCTAGTAAATTTGTATGGATTCTGCACCGAGGGAGCTAAACGGCTCCTTGTCTATGAGTATCTGGAGAACAAGAGTCTTGATCAAGCATTATTTGGAAAAAGAACCTTGCATCTTGATTGGCAGACACGTTTTGATATATGCTTGGGTGTAGCTAGAGGTTTAACTTATCTTCATGAAGAATCAAGGCTTCGAATTGTACACCGAGATGTGAAGGCAAGTAACATTCTGCTTGACTCTAATCTCATTGCCAAAATATCAGATTTTGGTTTGGCGAAGCATTATGATGATAAAAAGACCCACCTGAGTACTCGGGTTGCGGGAACAATTGGGTATCTTGCACCGGAATATGCTATGCGTGGACACTTGACAGAGAAAACCGATGTGTTTGCCTATGGTGTTGTCATTCTAGAAATTGTCAGCGGTAGGCCAAATTCTGATCCAAGTTTGGAAGGCGATATGGTTTATCTTCTTGAATTGGCTTGGAACTTGCATGAAAACGAACGTGAAGTTGATCTAGTAGACTCTAGATTATCCGAATTCAATGAGGAAGAAGCAAGACGAATTATCAACATAGGACTTCTGTGCACTCAATCATCACCAATGCTACGACCACCTATGTCTCGCGTGATTGGAATGCTTGCAGGAGATATTGAAGTGACTCCTGCTATTTCAAAGCCCGGTTACTTGACAGACTGGAGATTTGATGATGCAACTACTATCACCAGCCAAACGAAATATGGGAGTAGTGGCACCAGTCTAGGAACTGATATGTCAACTAGAGGAACTGACTTCAGCTTTTACAACTCATCGGCAAGTACAAGCGTGATGGGGGATGCAGGGCAATTACCTTCAAATGCCACTCTTCCCATACTCAATAATACTAATGGTGATGGTAGGTGA

Protein Analysis

1149

Amino Acids

126.36

Weight (kDa)

5.71

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 80 - 172 1.3e-08 Leucine-rich repeat region
Malectin PF11721 484 - 678 4.3e-39 Malectin domain
PK_Tyr_Ser-Thr PF07714 798 - 1060 2.7e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 798 - 995 3.2e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2067
AccB1I GGYRCC 1 cut(s) 3299
AccI GTMKAC 5 cut(s) 1381, 1637, 1920, 1993, 3069
AccII CGCG 1 cut(s) 3174
AciI CCGC 6 cut(s) 176, 198, 525, 1464, 2867, 2970
AclWI GGATC 6 cut(s) 83, 152, 1086, 1099, 1709, 2981
AcoI YGGCCR 2 cut(s) 141, 963
AcsI RAATTY 5 cut(s) 1769, 2150, 2557, 2980, 3086
AcuI CTGAAG 7 cut(s) 117, 958, 1371, 1457, 1656, 2384, 3325
AdeI CACNNNGTG 1 cut(s) 2110
AfaI GTAC 5 cut(s) 2008, 2437, 2748, 2857, 3367
AfeI AGCGCT 1 cut(s) 108
AfiI CCNNNNNNNGG 5 cut(s) 473, 805, 1227, 1408, 2003
AflIII ACRYGT 2 cut(s) 1822, 2675
AjiI CACGTC 1 cut(s) 1823
AjnI CCWGG 5 cut(s) 813, 1050, 1842, 2044, 2115
AloI GAACNNNNNNTCC 2 cut(s) 1770, 1802
Alw21I GWGCWC 3 cut(s) 330, 2115, 3137
Alw26I GTCTC 6 cut(s) 188, 735, 945, 1200, 1877, 3174
Alw44I GTGCAC 1 cut(s) 3133
AlwI GGATC 6 cut(s) 83, 152, 1086, 1099, 1709, 2981
Ama87I CYCGRG 1 cut(s) 2859
Aor51HI AGCGCT 1 cut(s) 108
AoxI GGCC 4 cut(s) 141, 963, 2420, 2975
ApaLI GTGCAC 1 cut(s) 3133
ApoI RAATTY 5 cut(s) 1769, 2150, 2557, 2980, 3086
ArsI GACNNNNNNTTYG 4 cut(s) 2148, 2180, 3233, 3265
Asp700I GAANNNNTTC 1 cut(s) 1669
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 6 cut(s) 1056, 1620, 2015, 2073, 2336, 2420
AsuC2I CCSGG 1 cut(s) 3228
AsuHPI GGTGA 8 cut(s) 167, 403, 539, 803, 1178, 3138, 3262, 3449
AsuII TTCGAA 1 cut(s) 2740
AsuNHI GCTAGC 1 cut(s) 1903
AvaI CYCGRG 1 cut(s) 2859
AvaII GGWCC 5 cut(s) 1056, 1620, 2015, 2073, 2336
BaeGI GKGCMC 1 cut(s) 3137
BalI TGGCCA 1 cut(s) 965
BamHI GGATCC 1 cut(s) 1091
BanI GGYRCC 1 cut(s) 3299
BanII GRGCYC 3 cut(s) 330, 1222, 1521
BbsI GAAGAC 3 cut(s) 1463, 1825, 2419
Bbv12I GWGCWC 3 cut(s) 330, 2115, 3137
BbvCI CCTCAGC 1 cut(s) 749
BccI CCATC 9 cut(s) 4, 466, 1221, 1327, 1554, 2039, 2441, 3370, 3434
BceAI ACGGC 3 cut(s) 156, 2447, 2606
BcgI CGANNNNNNTGC 2 cut(s) 2918, 2952
BciT130I CCWGG 5 cut(s) 815, 1052, 1844, 2046, 2117
BciVI GTATCC 1 cut(s) 597
BclI TGATCA 1 cut(s) 2629
BcnI CCSGG 1 cut(s) 3228
BcoDI GTCTC 6 cut(s) 188, 735, 945, 1200, 1877, 3174
BfaI CTAG 9 cut(s) 1790, 1904, 2552, 2703, 2957, 3065, 3075, 3308, 3327
BfmI CTRYAG 3 cut(s) 30, 40, 2145
BfoI RGCGCY 1 cut(s) 110
BfuI GTATCC 1 cut(s) 597
BglII AGATCT 1 cut(s) 2329
BmcAI AGTACT 1 cut(s) 2857
Bme1390I CCNGG 6 cut(s) 815, 1052, 1844, 2046, 2117, 3228
Bme18I GGWCC 5 cut(s) 1056, 1620, 2015, 2073, 2336
BmeT110I CYCGRG 1 cut(s) 2859
BmgBI CACGTC 1 cut(s) 1823
BmgT120I GGNCC 6 cut(s) 1056, 1620, 2015, 2073, 2336, 2420
BmiI GGNNCC 6 cut(s) 1057, 1093, 1219, 1621, 2594, 3301
BmrFI CCNGG 6 cut(s) 815, 1052, 1844, 2046, 2117, 3228
BmrI ACTGGG 2 cut(s) 1626, 2211
BmsI GCATC 5 cut(s) 1613, 2489, 2668, 3247, 3373
BmtI GCTAGC 1 cut(s) 1907
BmuI ACTGGG 2 cut(s) 1626, 2211
BoxI GACNNNNGTC 2 cut(s) 1173, 2385
BpiI GAAGAC 3 cut(s) 1463, 1825, 2419
BpmI CTGGAG 3 cut(s) 2099, 2633, 3265
Bpu10I CCTNAGC 2 cut(s) 749, 1929
Bpu14I TTCGAA 1 cut(s) 2740
BpuEI CTTGAG 2 cut(s) 1156, 1760
BpuMI CCSGG 1 cut(s) 3228
BsaBI GATNNNNATC 1 cut(s) 222
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 6 cut(s) 1050, 1051, 1222, 1522, 2491, 2577
BsaWI WCCGGW 2 cut(s) 607, 2890
BsaXI ACNNNNNCTCC 4 cut(s) 1197, 1227, 1639, 1669
Bsc4I CCNNNNNNNGG 5 cut(s) 473, 805, 1227, 1408, 2003
Bse3DI GCAATG 2 cut(s) 1579, 2793
Bse8I GATNNNNATC 1 cut(s) 222
BseBI CCWGG 5 cut(s) 815, 1052, 1844, 2046, 2117
BseDI CCNNGG 6 cut(s) 1050, 1051, 1222, 1522, 2491, 2577
BseGI GGATG 6 cut(s) 1232, 1305, 1338, 1852, 2483, 3388
BseJI GATNNNNATC 1 cut(s) 222
BseLI CCNNNNNNNGG 5 cut(s) 473, 805, 1227, 1408, 2003
BseMI GCAATG 2 cut(s) 1579, 2793
BseMII CTCAG 7 cut(s) 459, 740, 915, 1732, 1943, 2505, 2843
BseRI GAGGAG 2 cut(s) 218, 2015
BseSI GKGCMC 1 cut(s) 3137
BsgI GTGCAG 2 cut(s) 1548, 2557
Bsh1236I CGCG 1 cut(s) 3174
BshFI GGCC 4 cut(s) 143, 965, 2422, 2977
BshNI GGYRCC 1 cut(s) 3299
BsiHKAI GWGCWC 3 cut(s) 330, 2115, 3137
BsiHKCI CYCGRG 1 cut(s) 2859
BsiSI CCGG 3 cut(s) 608, 2891, 3228
BslFI GGGAC 6 cut(s) 378, 451, 1069, 1507, 1633, 1996
BslI CCNNNNNNNGG 5 cut(s) 473, 805, 1227, 1408, 2003
BsmAI GTCTC 6 cut(s) 188, 735, 945, 1200, 1877, 3174
BsmBI CGTCTC 1 cut(s) 945
BsmFI GGGAC 6 cut(s) 378, 451, 1069, 1507, 1633, 1996
BsmI GAATGC 3 cut(s) 118, 1040, 3189
BsnI GGCC 4 cut(s) 143, 965, 2422, 2977
Bso31I GGTCTC 1 cut(s) 188
BsoBI CYCGRG 1 cut(s) 2859
Bsp119I TTCGAA 1 cut(s) 2740
Bsp1286I GDGCHC 5 cut(s) 330, 1222, 1521, 2115, 3137
Bsp1407I TGTACA 1 cut(s) 2746
Bsp143I GATC 9 cut(s) 88, 157, 169, 1091, 1701, 2329, 2629, 2986, 3061
Bsp19I CCATGG 1 cut(s) 1222
BspACI CCGC 6 cut(s) 176, 198, 525, 1464, 2867, 2970
BspANI GGCC 4 cut(s) 143, 965, 2422, 2977
BspCNI CTCAG 7 cut(s) 460, 741, 916, 1731, 1942, 2506, 2844
BspFNI CGCG 1 cut(s) 3174
BspHI TCATGA 2 cut(s) 1452, 2722
BspLI GGNNCC 6 cut(s) 1057, 1093, 1219, 1621, 2594, 3301
BspMAI CTGCAG 2 cut(s) 34, 44
BspOI GCTAGC 1 cut(s) 1907
BspPI GGATC 6 cut(s) 83, 152, 1086, 1099, 1709, 2981
BspQI GCTCTTC 3 cut(s) 318, 1307, 2307
BspT104I TTCGAA 1 cut(s) 2740
BspT107I GGYRCC 1 cut(s) 3299
BspTNI GGTCTC 1 cut(s) 188
BsrDI GCAATG 2 cut(s) 1579, 2793
BsrGI TGTACA 1 cut(s) 2746
BssECI CCNNGG 6 cut(s) 1050, 1051, 1222, 1522, 2491, 2577
BssMI GATC 9 cut(s) 88, 157, 169, 1091, 1701, 2329, 2629, 2986, 3061
BssNAI GTATAC 3 cut(s) 1638, 1921, 1994
BssT1I CCWWGG 3 cut(s) 1222, 1522, 2491
Bst1107I GTATAC 3 cut(s) 1638, 1921, 1994
Bst2UI CCWGG 5 cut(s) 815, 1052, 1844, 2046, 2117
Bst4CI ACNGT 7 cut(s) 986, 1427, 1498, 1593, 1759, 2110, 2164
Bst6I CTCTTC 9 cut(s) 186, 318, 706, 948, 1123, 1307, 2307, 2446, 3417
BstAUI TGTACA 1 cut(s) 2746
BstBI TTCGAA 1 cut(s) 2740
BstC8I GCNNGC 4 cut(s) 40, 879, 1905, 3189
BstDEI CTNAG 8 cut(s) 468, 521, 749, 924, 1718, 1929, 2514, 2852
BstDSI CCRYGG 1 cut(s) 1222
BstF5I GGATG 6 cut(s) 1232, 1305, 1338, 1852, 2483, 3388
BstFNI CGCG 1 cut(s) 3174
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 9 cut(s) 91, 160, 172, 1094, 1704, 2332, 2632, 2989, 3064
BstMAI GTCTC 6 cut(s) 188, 735, 945, 1200, 1877, 3174
BstMBI GATC 9 cut(s) 88, 157, 169, 1091, 1701, 2329, 2629, 2986, 3061
BstMWI GCNNNNNNNGC 7 cut(s) 35, 113, 149, 1256, 1419, 1781, 2036
BstNI CCWGG 5 cut(s) 815, 1052, 1844, 2046, 2117
BstPAI GACNNNNGTC 2 cut(s) 1173, 2385
BstSCI CCNGG 6 cut(s) 813, 1050, 1842, 2044, 2115, 3226
BstSFI CTRYAG 3 cut(s) 30, 40, 2145
BstSLI GKGCMC 1 cut(s) 3137
BstUI CGCG 1 cut(s) 3174
BstV2I GAAGAC 3 cut(s) 1463, 1825, 2419
BstX2I RGATCY 4 cut(s) 88, 1091, 1701, 2329
BstYI RGATCY 4 cut(s) 88, 1091, 1701, 2329
BstZ17I GTATAC 3 cut(s) 1638, 1921, 1994
BsuI GTATCC 1 cut(s) 597
BsuRI GGCC 4 cut(s) 143, 965, 2422, 2977
BtgI CCRYGG 1 cut(s) 1222
BtrI CACGTC 1 cut(s) 1823
BtsCI GGATG 6 cut(s) 1232, 1305, 1338, 1852, 2483, 3388
BtsI GCAGTG 1 cut(s) 172
BtsIMutI CAGTG 8 cut(s) 5, 172, 594, 1066, 1102, 2041, 2106, 2292
Cac8I GCNNGC 4 cut(s) 40, 879, 1905, 3189
CciI TCATGA 2 cut(s) 1452, 2722
CfoI GCGC 1 cut(s) 109
Cfr13I GGNCC 6 cut(s) 1056, 1620, 2015, 2073, 2336, 2420
CseI GACGC 1 cut(s) 1690
CsiI ACCWGGT 1 cut(s) 2044
Csp6I GTAC 5 cut(s) 2007, 2436, 2747, 2856, 3366
CviAII CATG 6 cut(s) 162, 209, 1223, 1453, 2723, 3041
CviQI GTAC 5 cut(s) 2007, 2436, 2747, 2856, 3366
DdeI CTNAG 8 cut(s) 468, 521, 749, 924, 1718, 1929, 2514, 2852
DpnI GATC 9 cut(s) 90, 159, 171, 1093, 1703, 2331, 2631, 2988, 3063
DpnII GATC 9 cut(s) 88, 157, 169, 1091, 1701, 2329, 2629, 2986, 3061
DraI TTTAAA 1 cut(s) 483
DraIII CACNNNGTG 1 cut(s) 2110
EaeI YGGCCR 2 cut(s) 141, 963
Eam1104I CTCTTC 9 cut(s) 186, 318, 706, 948, 1123, 1307, 2307, 2446, 3417
EarI CTCTTC 9 cut(s) 186, 318, 706, 948, 1123, 1307, 2307, 2446, 3417
Ecl136II GAGCTC 1 cut(s) 328
Eco130I CCWWGG 3 cut(s) 1222, 1522, 2491
Eco24I GRGCYC 3 cut(s) 330, 1222, 1521
Eco31I GGTCTC 1 cut(s) 188
Eco32I GATATC 1 cut(s) 1485
Eco47I GGWCC 5 cut(s) 1056, 1620, 2015, 2073, 2336
Eco47III AGCGCT 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 328
Eco57I CTGAAG 7 cut(s) 117, 958, 1371, 1457, 1656, 2384, 3325
Eco88I CYCGRG 1 cut(s) 2859
EcoICRI GAGCTC 1 cut(s) 328
EcoRI GAATTC 2 cut(s) 2150, 3086
EcoRII CCWGG 5 cut(s) 813, 1050, 1842, 2044, 2115
EcoRV GATATC 1 cut(s) 1485
EcoT14I CCWWGG 3 cut(s) 1222, 1522, 2491
EcoT38I GRGCYC 3 cut(s) 330, 1222, 1521
ErhI CCWWGG 3 cut(s) 1222, 1522, 2491
Esp3I CGTCTC 1 cut(s) 945
FaeI CATG 6 cut(s) 165, 212, 1226, 1456, 2726, 3044
FaqI GGGAC 6 cut(s) 378, 451, 1069, 1507, 1633, 1996
FatI CATG 6 cut(s) 161, 208, 1222, 1452, 2722, 3040
FauI CCCGC 1 cut(s) 2860
FbaI TGATCA 1 cut(s) 2629
FblI GTMKAC 5 cut(s) 1381, 1637, 1920, 1993, 3069
FokI GGATG 6 cut(s) 1239, 1312, 1345, 1859, 2470, 3395
FriOI GRGCYC 3 cut(s) 330, 1222, 1521
FspBI CTAG 9 cut(s) 1790, 1904, 2552, 2703, 2957, 3065, 3075, 3308, 3327
GlaI GCGC 1 cut(s) 108
GsuI CTGGAG 3 cut(s) 2099, 2633, 3265
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 4 cut(s) 143, 965, 2422, 2977
HapII CCGG 3 cut(s) 608, 2891, 3228
HgaI GACGC 1 cut(s) 1690
HhaI GCGC 1 cut(s) 109
Hin1II CATG 6 cut(s) 165, 212, 1226, 1456, 2726, 3044
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HincII GTYRAC 4 cut(s) 648, 955, 2125, 3324
HindII GTYRAC 4 cut(s) 648, 955, 2125, 3324
HindIII AAGCTT 2 cut(s) 1763, 2399
HpaI GTTAAC 1 cut(s) 955
HpaII CCGG 3 cut(s) 608, 2891, 3228
HphI GGTGA 8 cut(s) 167, 403, 539, 803, 1178, 3138, 3262, 3449
Hpy99I CGWCG 1 cut(s) 1684
HpyCH4III ACNGT 7 cut(s) 986, 1427, 1498, 1593, 1759, 2110, 2164
HpyCH4IV ACGT 3 cut(s) 1822, 2677, 3052
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 113, 149, 1256, 1419, 1781, 2036
HpyF3I CTNAG 8 cut(s) 468, 521, 749, 924, 1718, 1929, 2514, 2852
HpySE526I ACGT 3 cut(s) 1822, 2677, 3052
Hsp92II CATG 6 cut(s) 165, 212, 1226, 1456, 2726, 3044
HspAI GCGC 1 cut(s) 107
Ksp22I TGATCA 1 cut(s) 2629
KspAI GTTAAC 1 cut(s) 955
Kzo9I GATC 9 cut(s) 88, 157, 169, 1091, 1701, 2329, 2629, 2986, 3061
LguI GCTCTTC 3 cut(s) 318, 1307, 2307
LmnI GCTCC 5 cut(s) 316, 1217, 2118, 2582, 2598
LweI GCATC 5 cut(s) 1613, 2489, 2668, 3247, 3373
MabI ACCWGGT 1 cut(s) 2044
MaeI CTAG 9 cut(s) 1790, 1904, 2552, 2703, 2957, 3065, 3075, 3308, 3327
MaeII ACGT 3 cut(s) 1822, 2677, 3052
MaeIII GTNAC 7 cut(s) 3, 1166, 1543, 2509, 2768, 3205, 3230
MalI GATC 9 cut(s) 90, 159, 171, 1093, 1703, 2331, 2631, 2988, 3063
MboI GATC 9 cut(s) 88, 157, 169, 1091, 1701, 2329, 2629, 2986, 3061
MfeI CAATTG 2 cut(s) 2471, 2874
MflI RGATCY 4 cut(s) 88, 1091, 1701, 2329
MhlI GDGCHC 5 cut(s) 330, 1222, 1521, 2115, 3137
MlsI TGGCCA 1 cut(s) 965
MluNI TGGCCA 1 cut(s) 965
MlyI GAGTC 7 cut(s) 643, 935, 1163, 2632, 2777, 3065, 3202
MmeI TCCRAC 5 cut(s) 807, 1092, 1183, 1195, 1261
Mox20I TGGCCA 1 cut(s) 965
MroXI GAANNNNTTC 1 cut(s) 1669
MscI TGGCCA 1 cut(s) 965
MslI CAYNNNNRTG 3 cut(s) 213, 2754, 2832
Msp20I TGGCCA 1 cut(s) 965
MspA1I CMGCKG 2 cut(s) 152, 2970
MspI CCGG 3 cut(s) 608, 2891, 3228
MspR9I CCNGG 6 cut(s) 815, 1052, 1844, 2046, 2117, 3228
MunI CAATTG 2 cut(s) 2471, 2874
Mva1269I GAATGC 3 cut(s) 118, 1040, 3189
MvaI CCWGG 5 cut(s) 815, 1052, 1844, 2046, 2117
MvnI CGCG 1 cut(s) 3174
MwoI GCNNNNNNNGC 7 cut(s) 35, 113, 149, 1256, 1419, 1781, 2036
NciI CCSGG 1 cut(s) 3228
NcoI CCATGG 1 cut(s) 1222
NdeII GATC 9 cut(s) 88, 157, 169, 1091, 1701, 2329, 2629, 2986, 3061
NheI GCTAGC 1 cut(s) 1903
NlaIII CATG 6 cut(s) 165, 212, 1226, 1456, 2726, 3044
NlaIV GGNNCC 6 cut(s) 1057, 1093, 1219, 1621, 2594, 3301
NmuCI GTSAC 5 cut(s) 3, 1166, 1543, 2509, 3205
NspV TTCGAA 1 cut(s) 2740
PagI TCATGA 2 cut(s) 1452, 2722
PasI CCCWGGG 1 cut(s) 1051
PciSI GCTCTTC 3 cut(s) 318, 1307, 2307
PctI GAATGC 3 cut(s) 118, 1040, 3189
PdmI GAANNNNTTC 1 cut(s) 1669
PfeI GAWTC 5 cut(s) 254, 532, 770, 2568, 2729
PfoI TCCNGGA 1 cut(s) 1842
PleI GAGTC 7 cut(s) 643, 934, 1163, 2631, 2777, 3065, 3202
PpsI GAGTC 7 cut(s) 643, 934, 1163, 2631, 2777, 3065, 3202
PshAI GACNNNNGTC 2 cut(s) 1173, 2385
PsiI TTATAA 1 cut(s) 2067
Psp124BI GAGCTC 1 cut(s) 330
Psp6I CCWGG 5 cut(s) 813, 1050, 1842, 2044, 2115
PspGI CCWGG 5 cut(s) 813, 1050, 1842, 2044, 2115
PspN4I GGNNCC 6 cut(s) 1057, 1093, 1219, 1621, 2594, 3301
PspPI GGNCC 6 cut(s) 1056, 1620, 2015, 2073, 2336, 2420
PstI CTGCAG 2 cut(s) 34, 44
PsuI RGATCY 4 cut(s) 88, 1091, 1701, 2329
PvuII CAGCTG 1 cut(s) 152
RsaI GTAC 5 cut(s) 2008, 2437, 2748, 2857, 3367
RsaNI GTAC 5 cut(s) 2007, 2436, 2747, 2856, 3366
RseI CAYNNNNRTG 3 cut(s) 213, 2754, 2832
SacI GAGCTC 1 cut(s) 330
SapI GCTCTTC 3 cut(s) 318, 1307, 2307
Sau3AI GATC 9 cut(s) 88, 157, 169, 1091, 1701, 2329, 2629, 2986, 3061
Sau96I GGNCC 6 cut(s) 1056, 1620, 2015, 2073, 2336, 2420
ScaI AGTACT 1 cut(s) 2857
SchI GAGTC 7 cut(s) 643, 935, 1163, 2632, 2777, 3065, 3202
ScrFI CCNGG 6 cut(s) 815, 1052, 1844, 2046, 2117, 3228
SduI GDGCHC 5 cut(s) 330, 1222, 1521, 2115, 3137
SexAI ACCWGGT 1 cut(s) 2044
SfaNI GCATC 5 cut(s) 1613, 2489, 2668, 3247, 3373
SfcI CTRYAG 3 cut(s) 30, 40, 2145
SfuI TTCGAA 1 cut(s) 2740
SinI GGWCC 5 cut(s) 1056, 1620, 2015, 2073, 2336
SmiMI CAYNNNNRTG 3 cut(s) 213, 2754, 2832
SmlI CTYRAG 2 cut(s) 1171, 1739
SmoI CTYRAG 2 cut(s) 1171, 1739
SsiI CCGC 6 cut(s) 176, 198, 525, 1464, 2867, 2970
SspI AATATT 3 cut(s) 136, 570, 763
SspMI CTAG 9 cut(s) 1790, 1904, 2552, 2703, 2957, 3065, 3075, 3308, 3327
SstI GAGCTC 1 cut(s) 330
StyD4I CCNGG 6 cut(s) 813, 1050, 1842, 2044, 2115, 3226
StyI CCWWGG 3 cut(s) 1222, 1522, 2491
TaaI ACNGT 7 cut(s) 986, 1427, 1498, 1593, 1759, 2110, 2164
TaiI ACGT 3 cut(s) 1825, 2680, 3055
TaqI TCGA 2 cut(s) 2539, 2740
TaqII GACCGA 1 cut(s) 1568
TatI WGTACW 3 cut(s) 2746, 2855, 3365
TfiI GAWTC 5 cut(s) 254, 532, 770, 2568, 2729
TscAI CASTG 8 cut(s) 12, 172, 601, 1066, 1102, 2041, 2113, 2299
TseFI GTSAC 5 cut(s) 3, 1166, 1543, 2509, 3205
Tsp45I GTSAC 5 cut(s) 3, 1166, 1543, 2509, 3205
TspGWI ACGGA 1 cut(s) 1891
TspRI CASTG 8 cut(s) 12, 172, 601, 1066, 1102, 2041, 2113, 2299
VneI GTGCAC 1 cut(s) 3133
VpaK11BI GGWCC 5 cut(s) 1056, 1620, 2015, 2073, 2336
XapI RAATTY 5 cut(s) 1769, 2150, 2557, 2980, 3086
XbaI TCTAGA 2 cut(s) 2956, 3074
XcmI CCANNNNNNNNNTGG 1 cut(s) 3284
XmiI GTMKAC 5 cut(s) 1381, 1637, 1920, 1993, 3069
XmnI GAANNNNTTC 1 cut(s) 1669
XspI CTAG 9 cut(s) 1790, 1904, 2552, 2703, 2957, 3065, 3075, 3308, 3327
ZrmI AGTACT 1 cut(s) 2857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.