MD05G1260400.v1.1

Di-glucose binding within endoplasmic reticulum

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000088
Physical Location & Seq
Reverse (-)
39553171 .. 39569185
16015 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1260400.v1.1.491

Sequence Viewer

Length: 3144 bp
ATGTACAATATGAAGATGAAGTTGATGATGAGGATACTATTACTCTACTTCCTCAGCTACTTCTGGTTTCAGCTGTCATTTGCTCAAAATGCTACCACCGATCCATCTGAAGTGAGGGCGTTGAACTCAATATTTGAACAATGGGACACACAAGCAGTGCCGGGGCTGTGGAATATCAGTGGAGAACCCTGCAGTGGATCTGCCATCAACGGCACCGACTTTGGGGACCCCAGTAACAACCCAGCCATCGTTTGCGACTGTTCTTACGACAACAATGCTACATGCCACATCACCAAACTGAGAGTGTATGCTCTGAACAAACGAGGGGTGTTTCCAGAAGAATTTGTGGCTCTGAGATATCTCACATCTTTGAAAATCGATCAGAATTATTTCACCGGTCCCCTACCAGCATTCATTGGCAATATGTCTGCATTGACTGAATTGTCAGTTGGCATCAATTCATTCTCTGGACCCATCCCCAAGGAGCTTGGAAACCTTACGGAGCTAACTCTGCTGGGCATCGGATCAAATAATTTCTCCGGAACACTCCCTCCAGAACTTGGTAATTTAGTCAAGCTCGAGCAAATTTACATGGACAGCTGTGGACTCAGTGGTGAAATTCCTTCAACATTCGCCAAGCTCACCAACATGCAAACCCTTTGGGCATCGGACAGTCTTTTCTCAGGAAAGACACCTGATTTCATAGGGAATTGGACACAACTCACTTCTTTGCGATTTCAAGGGAACTCTTTCGAAGGCCCAATACCAACCAGCTTTTCTCAGCTGACCTCATTGACCTCTCTGCGAATCAGTGATATATACAATGGGAGCTCCTCTCTTGATTTCATAAAAAATCTGAAAAGCTTGACTGAATTAAAACTGCGAAACGCATTAATCACTGGTACCATCCCATCTGATATTGGAGAATATCAAAGTCTACAGACACTGGATCTGAGTTTCAACAATTTGACAGGCCAACTCCCAAGTTCTTTGTTCAACTTGAGTTCTCTTACATCCTTGTTTCTTGGAAACAATAGTCTGTCCGGACCTCTTCCAAGCCAAAAGAGCAATCAACTTGAGACTATAGATTTGTCTTACAATTTTTTATCAGGAAGCTTTCCCCAGTGGGTGACCACAATATCGCAACTGAACTTAGTGGTCAACAACTTCACATTTGACAGTTCAAACATAACTCTTCCTGGATTGGATTGCCTCCAGAGAAATTTTCCATGCAATCGAAATGCCCCACGATATGCAAACTTCTCAATCAACTGTGGTGGAAAACAAATGACGGGAAGTGATGGCATATTGTATGAGACTGAAGACTCAGCTCTTGGCGCAGCAACATTCAATGTAACTAGTACAGAGAAATGGGCTGTCAGCAATGCCGGTTTGTTTTCTGACAGAAAGGACCCGTCGTTTCTGGAAAATACCCTTGCACAAGTCACCGGAACGGATGTGACCCCGGAGCTTTTCCAGACTTCAAGAGTGTCCCCAGGATCACTGAGATACTATGGCCTGAGCCTTGAGAATGGGCCTTACACTGTAACATTGCACTTTGCAGAGACGGTTTATGAAAGTCGCACTTCGCAAAAATGGCAAAGTCTAGGACGGCGTGTATTTGATATCTATATTCAGGGTACCCGCATGACGAAGGACTTGGACATATCGTCGGCGGCAGGTGGTGTTAACCGAGCAATTGTGAGAAAATTTAATGTTAACGTGTCAGAGAATTATCTTGAAATTCATCTGTTCTGGGCTGGTAAAGGGACTTGTTGCATACCCGAACAAGGTGATTACGGCCCGCTAATAGCAGCTGTCCATGCTGCTTCAGATTTTACACCAACAGTTGCCGGGCTTCCACCAACTACTCCAGGAAAGAAGAGCAAGACTGGGTTGATAGTCGGTATTGCAGTTCCTATTGGAGTTGTGAGCTTGCTATTAATATTTGCAATTCTATATATGAGGAGGAAAAAATCAGAAAAAGAAGACGATGAAGATATTCTAGGATTAGGCCCCCGACCATATACTTTCAGTTATGCTGAATTGAGAACCGCAACTGAAGATTTTAATACTTCAAATAAGCTAGGAGAGGGAGGATATGGCCCTGTTTATAAGGGTGCACTTTCTGATGGGAGAATAGTGGCTGTGAAGCAACTTTCAGTAGCATCTCACCAAGGAAAGAGTCAATTTGTATCTGAAATTGCTACCATATCTGCAGTGCAACATCGGAATCTAGTGAAATTGTATGGATGCTGCATCGAAGGCAGCCAGCGCATTTTGGTTTATGAGTATCTTGAAAACAAGAGCCTTGATCAGGCACTTTTTGGGACAAGTAACTTGCACCTTGACTGGCCTACTCGATTCAATATATTGTTGGGAACAGCAAGAGGACTTGCTTACCTTCACGAGGAGTCAAGGCCAAGGATTGTACATCGAGATGTTAAGGCCAGTAATATTTTGCTCGATGCAGAACTCTCCCCAAAAATATCAGATTTTGGATTGGCAAAGCTTTATGATGACGAGAAAACCCACATCAGCACCCGGGTTGCAGGGACAATAGGCTATTTGGCACCAGAATATGCAATGCGTGGACATTTGACAGAGAAGGCTGATGTGTTTGGTTTTGGAGTCGTTGCTTTGGAGATCCTCAGCGGGAGACCAAACTCTGACAATAACTTAGATCCAGAAAAGATTTATCTTCTTGAATGGGCATGGACTCTACATGAAAACGACCAAATTCTGGGGCTGGTGGATTCAAGATTGACAGAGTTTGACGAAACTGAAGCGACTAGAGTGATAAGAGCAGCTCTCCTGTGCACGCAGGCATCACCGATGACGAGGCCATCTATGTCACGCATGGTGGCAATGCTCTCTGGAGATATTGACATAGGCACTGTCATGTCGAAGCCGAGCTATTTGACAGATTATGATTTTAAAGATGTAACGACATTGTCAACAGGTAGCTTTTTGATGGAGGATGATACCCCATCAACTGCATCCAAGTACAGTAATGTTCGTCTCAACTATCAGCCCGAAGGCAACAGTGCAAGTGGTGCTAACACCCCCGAGGTTGACCTTGCGCCTTCTCCTGTAAACGTCACTCAATCACTGCTCACTGGAATTATAGGAGAAGGAAGGTGA

Protein Analysis

1048

Amino Acids

114.8

Weight (kDa)

5.16

Isoelectric Point (pI)

34.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 110 - 175 4.2e-06 Leucine-rich repeat region
LRR_14 PF23598 181 - 270 2.6e-06 Leucine-rich repeat region
LRR_14 PF23598 264 - 361 1.2e-08 Leucine-rich repeat region
Malectin PF11721 421 - 607 2.2e-38 Malectin domain
Pkinase PF00069 691 - 954 2.7e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 692 - 957 2.7e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2115
AarI CACCTGC 1 cut(s) 1670
AasI GACNNNNNNGTC 1 cut(s) 442
Acc36I ACCTGC 1 cut(s) 1670
Acc65I GGTACC 2 cut(s) 902, 1640
AccB1I GGYRCC 4 cut(s) 212, 902, 1640, 2572
AccB7I CCANNNNNTGG 2 cut(s) 560, 2743
AccI GTMKAC 1 cut(s) 937
AccIII TCCGGA 2 cut(s) 539, 1043
AciI CCGC 5 cut(s) 1645, 1676, 1805, 2055, 2655
AclWI GGATC 7 cut(s) 95, 205, 532, 957, 1507, 2641, 2678
AcsI RAATTY 7 cut(s) 341, 585, 618, 1222, 1709, 1743, 2739
AcuI CTGAAG 5 cut(s) 129, 1341, 1815, 2082, 2805
AfaI GTAC 6 cut(s) 5, 904, 1363, 1642, 2433, 3008
AfiI CCNNNNNNNGG 7 cut(s) 194, 222, 560, 1790, 2317, 2410, 2743
AflIII ACRYGT 1 cut(s) 1722
AgeI ACCGGT 1 cut(s) 395
AhdI GACNNNNNGTC 1 cut(s) 1669
AhlI ACTAGT 1 cut(s) 1358
AjnI CCWGG 3 cut(s) 1198, 1495, 1873
AjuI GAANNNNNNNTTGG 4 cut(s) 432, 464, 760, 792
AloI GAACNNNNNNTCC 2 cut(s) 535, 567
Alw21I GWGCWC 3 cut(s) 833, 2125, 2822
Alw26I GTCTC 5 cut(s) 1073, 1310, 1559, 2653, 3026
Alw44I GTGCAC 2 cut(s) 2121, 2818
AlwI GGATC 7 cut(s) 95, 205, 532, 957, 1507, 2641, 2678
AlwNI CAGNNNCTG 1 cut(s) 946
Ama87I CYCGRG 3 cut(s) 578, 2544, 3068
Aor13HI TCCGGA 2 cut(s) 539, 1043
ApaLI GTGCAC 2 cut(s) 2121, 2818
ApeKI GCWGC 6 cut(s) 1340, 1814, 1826, 2256, 2268, 2807
ApoI RAATTY 7 cut(s) 341, 585, 618, 1222, 1709, 1743, 2739
ArsI GACNNNNNNTTYG 2 cut(s) 1072, 1104
AseI ATTAAT 2 cut(s) 893, 1943
AsiGI ACCGGT 1 cut(s) 395
Asp700I GAANNNNTTC 3 cut(s) 389, 749, 2001
Asp718I GGTACC 2 cut(s) 902, 1640
AspLEI GCGC 3 cut(s) 1340, 2277, 3085
AsuC2I CCSGG 5 cut(s) 162, 1466, 1855, 2545, 2546
AsuHPI GGTGA 9 cut(s) 283, 385, 626, 634, 1141, 1438, 1805, 2165, 2823
AsuII TTCGAA 1 cut(s) 753
AvaI CYCGRG 3 cut(s) 578, 2544, 3068
AvaII GGWCC 5 cut(s) 226, 398, 470, 1046, 1411
BaeGI GKGCMC 2 cut(s) 2125, 2822
BanI GGYRCC 4 cut(s) 212, 902, 1640, 2572
BanII GRGCYC 1 cut(s) 833
BarI GAAGNNNNNNTAC 2 cut(s) 747, 779
BauI CACGAG 1 cut(s) 2408
BbsI GAAGAC 2 cut(s) 1329, 1995
Bbv12I GWGCWC 3 cut(s) 833, 2125, 2822
BbvCI CCTCAGC 2 cut(s) 53, 2651
BbvI GCAGC 6 cut(s) 1352, 1813, 1826, 2243, 2280, 2819
BceAI ACGGC 3 cut(s) 226, 1628, 1816
BcgI CGANNNNNNTGC 2 cut(s) 257, 291
BciT130I CCWGG 3 cut(s) 1200, 1497, 1875
BciVI GTATCC 1 cut(s) 27
BclI TGATCA 1 cut(s) 2314
BcnI CCSGG 5 cut(s) 162, 1466, 1855, 2545, 2546
BcoDI GTCTC 5 cut(s) 1073, 1310, 1559, 2653, 3026
BcuI ACTAGT 1 cut(s) 1358
BfaI CTAG 6 cut(s) 1359, 1607, 2006, 2087, 2237, 2793
BfmI CTRYAG 4 cut(s) 190, 938, 1083, 2217
BfuAI ACCTGC 1 cut(s) 1670
BfuI GTATCC 1 cut(s) 27
BisI GCNGC 7 cut(s) 1341, 1677, 1815, 1827, 2257, 2269, 2808
BlsI GCNGC 7 cut(s) 1342, 1678, 1816, 1828, 2258, 2270, 2809
Bme1390I CCNGG 8 cut(s) 162, 1200, 1466, 1497, 1855, 1875, 2545, 2546
Bme18I GGWCC 5 cut(s) 226, 398, 470, 1046, 1411
BmeRI GACNNNNNGTC 1 cut(s) 1669
BmeT110I CYCGRG 3 cut(s) 578, 2544, 3068
BmrFI CCNGG 8 cut(s) 162, 1200, 1466, 1497, 1855, 1875, 2545, 2546
BmrI ACTGGG 3 cut(s) 225, 1117, 1902
BmsI GCATC 9 cut(s) 462, 528, 674, 2177, 2243, 2268, 2458, 2837, 3008
BmuI ACTGGG 3 cut(s) 225, 1117, 1902
BpiI GAAGAC 2 cut(s) 1329, 1995
BplI GAGNNNNNCTC 4 cut(s) 820, 852, 2796, 2828
BpmI CTGGAG 4 cut(s) 537, 1199, 1857, 2898
Bpu10I CCTNAGC 3 cut(s) 53, 1520, 2651
Bpu14I TTCGAA 1 cut(s) 753
BpuEI CTTGAG 3 cut(s) 1021, 1097, 1547
BpuMI CCSGG 5 cut(s) 162, 1466, 1855, 2545, 2546
Bsa29I ATCGAT 1 cut(s) 378
BsaI GGTCTC 1 cut(s) 2653
BsaJI CCNNGG 8 cut(s) 161, 480, 1464, 1495, 2176, 2423, 2544, 3069
BsaWI WCCGGW 4 cut(s) 395, 539, 1043, 1448
BsaXI ACNNNNNCTCC 2 cut(s) 535, 565
Bsc4I CCNNNNNNNGG 7 cut(s) 194, 222, 560, 1790, 2317, 2410, 2743
Bse118I RCCGGY 2 cut(s) 395, 1388
Bse1I ACTGG 8 cut(s) 231, 904, 951, 1123, 1897, 2357, 2451, 3124
Bse3DI GCAATG 4 cut(s) 1390, 1550, 2592, 2874
BseAI TCCGGA 2 cut(s) 539, 1043
BseBI CCWGG 3 cut(s) 1200, 1497, 1875
BseCI ATCGAT 1 cut(s) 378
BseDI CCNNGG 8 cut(s) 161, 480, 1464, 1495, 2176, 2423, 2544, 3069
BseGI GGATG 7 cut(s) 474, 906, 1013, 1462, 2258, 2986, 2999
BseLI CCNNNNNNNGG 7 cut(s) 194, 222, 560, 1790, 2317, 2410, 2743
BseMI GCAATG 4 cut(s) 1390, 1550, 2592, 2874
BseNI ACTGG 8 cut(s) 231, 904, 951, 1123, 1897, 2357, 2451, 3124
BseRI GAGGAG 3 cut(s) 823, 1981, 2426
BseSI GKGCMC 2 cut(s) 2125, 2822
BseXI GCAGC 6 cut(s) 1352, 1813, 1826, 2243, 2280, 2819
BseYI CCCAGC 2 cut(s) 241, 514
BshNI GGYRCC 4 cut(s) 212, 902, 1640, 2572
BshTI ACCGGT 1 cut(s) 395
BshVI ATCGAT 1 cut(s) 378
BsiHKAI GWGCWC 3 cut(s) 833, 2125, 2822
BsiHKCI CYCGRG 3 cut(s) 578, 2544, 3068
BsiSI CCGG 9 cut(s) 161, 396, 540, 1044, 1389, 1449, 1466, 1854, 2545
BslFI GGGAC 7 cut(s) 158, 239, 384, 1477, 1783, 2344, 2569
BslI CCNNNNNNNGG 7 cut(s) 194, 222, 560, 1790, 2317, 2410, 2743
BsmAI GTCTC 5 cut(s) 1073, 1310, 1559, 2653, 3026
BsmBI CGTCTC 2 cut(s) 1559, 3026
BsmFI GGGAC 7 cut(s) 158, 239, 384, 1477, 1783, 2344, 2569
BsmI GAATGC 1 cut(s) 410
Bso31I GGTCTC 1 cut(s) 2653
BsoBI CYCGRG 3 cut(s) 578, 2544, 3068
Bsp119I TTCGAA 1 cut(s) 753
Bsp1286I GDGCHC 3 cut(s) 833, 2125, 2822
Bsp13I TCCGGA 2 cut(s) 539, 1043
Bsp1407I TGTACA 2 cut(s) 3, 2431
Bsp143I GATC 9 cut(s) 100, 197, 379, 524, 949, 1499, 2314, 2646, 2683
BspACI CCGC 5 cut(s) 1645, 1676, 1805, 2055, 2655
BspDI ATCGAT 1 cut(s) 378
BspEI TCCGGA 2 cut(s) 539, 1043
BspMAI CTGCAG 2 cut(s) 194, 2221
BspMI ACCTGC 1 cut(s) 1670
BspPI GGATC 7 cut(s) 95, 205, 532, 957, 1507, 2641, 2678
BspQI GCTCTTC 1 cut(s) 1877
BspT104I TTCGAA 1 cut(s) 753
BspT107I GGYRCC 4 cut(s) 212, 902, 1640, 2572
BspTNI GGTCTC 1 cut(s) 2653
BsrDI GCAATG 4 cut(s) 1390, 1550, 2592, 2874
BsrFI RCCGGY 2 cut(s) 395, 1388
BsrGI TGTACA 2 cut(s) 3, 2431
BsrI ACTGG 8 cut(s) 231, 904, 951, 1123, 1897, 2357, 2451, 3124
BssAI RCCGGY 2 cut(s) 395, 1388
BssECI CCNNGG 8 cut(s) 161, 480, 1464, 1495, 2176, 2423, 2544, 3069
BssMI GATC 9 cut(s) 100, 197, 379, 524, 949, 1499, 2314, 2646, 2683
BssSI CACGAG 1 cut(s) 2408
BssT1I CCWWGG 3 cut(s) 480, 2176, 2423
Bst2BI CACGAG 1 cut(s) 2408
Bst2UI CCWGG 3 cut(s) 1200, 1497, 1875
Bst6I CTCTTC 3 cut(s) 1056, 1200, 1877
BstAPI GCANNNNNTGC 1 cut(s) 3056
BstAUI TGTACA 2 cut(s) 3, 2431
BstBI TTCGAA 1 cut(s) 753
BstC8I GCNNGC 5 cut(s) 1805, 1937, 2273, 2822, 2826
BstEII GGTNACC 1 cut(s) 1129
BstENI CCTNNNNNAGG 2 cut(s) 2315, 2408
BstF5I GGATG 7 cut(s) 474, 906, 1013, 1462, 2258, 2986, 2999
BstHHI GCGC 3 cut(s) 1340, 2277, 3085
BstKTI GATC 9 cut(s) 103, 200, 382, 527, 952, 1502, 2317, 2649, 2686
BstMAI GTCTC 5 cut(s) 1073, 1310, 1559, 2653, 3026
BstMBI GATC 9 cut(s) 100, 197, 379, 524, 949, 1499, 2314, 2646, 2683
BstMWI GCNNNNNNNGC 9 cut(s) 89, 511, 1065, 1337, 1597, 1823, 2265, 2274, 3056
BstNI CCWGG 3 cut(s) 1200, 1497, 1875
BstNSI RCATGY 2 cut(s) 285, 652
BstPI GGTNACC 1 cut(s) 1129
BstSCI CCNGG 8 cut(s) 160, 1198, 1464, 1495, 1853, 1873, 2543, 2544
BstSFI CTRYAG 4 cut(s) 190, 938, 1083, 2217
BstSLI GKGCMC 2 cut(s) 2125, 2822
BstV1I GCAGC 6 cut(s) 1352, 1813, 1826, 2243, 2280, 2819
BstV2I GAAGAC 2 cut(s) 1329, 1995
BstX2I RGATCY 4 cut(s) 197, 949, 2646, 2683
BstYI RGATCY 4 cut(s) 197, 949, 2646, 2683
Bsu15I ATCGAT 1 cut(s) 378
BsuI GTATCC 1 cut(s) 27
BsuTUI ATCGAT 1 cut(s) 378
BtsCI GGATG 7 cut(s) 474, 906, 1013, 1462, 2258, 2986, 2999
BtsI GCAGTG 4 cut(s) 162, 199, 2226, 3110
BveI ACCTGC 1 cut(s) 1670
Cac8I GCNNGC 5 cut(s) 1805, 1937, 2273, 2822, 2826
CaiI CAGNNNCTG 1 cut(s) 946
CfoI GCGC 3 cut(s) 1340, 2277, 3085
Cfr10I RCCGGY 2 cut(s) 395, 1388
Cfr9I CCCGGG 1 cut(s) 2544
ClaI ATCGAT 1 cut(s) 378
Csp6I GTAC 6 cut(s) 4, 903, 1362, 1641, 2432, 3007
CspAI ACCGGT 1 cut(s) 395
CspCI CAANNNNNGTGG 2 cut(s) 1258, 1293
CviQI GTAC 6 cut(s) 4, 903, 1362, 1641, 2432, 3007
DpnI GATC 9 cut(s) 102, 199, 381, 526, 951, 1501, 2316, 2648, 2685
DpnII GATC 9 cut(s) 100, 197, 379, 524, 949, 1499, 2314, 2646, 2683
DraI TTTAAA 1 cut(s) 2938
DrdI GACNNNNNNGTC 1 cut(s) 442
DriI GACNNNNNGTC 1 cut(s) 1669
DseDI GACNNNNNNGTC 1 cut(s) 442
Eam1104I CTCTTC 3 cut(s) 1056, 1200, 1877
Eam1105I GACNNNNNGTC 1 cut(s) 1669
EarI CTCTTC 3 cut(s) 1056, 1200, 1877
Ecl136II GAGCTC 1 cut(s) 831
Eco130I CCWWGG 3 cut(s) 480, 2176, 2423
Eco24I GRGCYC 1 cut(s) 833
Eco31I GGTCTC 1 cut(s) 2653
Eco32I GATATC 2 cut(s) 359, 1627
Eco47I GGWCC 5 cut(s) 226, 398, 470, 1046, 1411
Eco53kI GAGCTC 1 cut(s) 831
Eco57I CTGAAG 5 cut(s) 129, 1341, 1815, 2082, 2805
Eco88I CYCGRG 3 cut(s) 578, 2544, 3068
Eco91I GGTNACC 1 cut(s) 1129
EcoICRI GAGCTC 1 cut(s) 831
EcoNI CCTNNNNNAGG 2 cut(s) 2315, 2408
EcoO109I RGGNCCY 3 cut(s) 226, 1411, 2015
EcoO65I GGTNACC 1 cut(s) 1129
EcoRII CCWGG 3 cut(s) 1198, 1495, 1873
EcoRV GATATC 2 cut(s) 359, 1627
EcoT14I CCWWGG 3 cut(s) 480, 2176, 2423
EcoT38I GRGCYC 1 cut(s) 833
ErhI CCWWGG 3 cut(s) 480, 2176, 2423
Esp3I CGTCTC 2 cut(s) 1559, 3026
FalI AAGNNNNNCTT 2 cut(s) 1570, 1602
FaqI GGGAC 7 cut(s) 158, 239, 384, 1477, 1783, 2344, 2569
FauI CCCGC 3 cut(s) 1652, 1812, 2648
FbaI TGATCA 1 cut(s) 2314
FblI GTMKAC 1 cut(s) 937
Fnu4HI GCNGC 7 cut(s) 1341, 1677, 1815, 1827, 2257, 2269, 2808
FokI GGATG 7 cut(s) 461, 893, 1000, 1469, 2265, 2986, 2993
FriOI GRGCYC 1 cut(s) 833
Fsp4HI GCNGC 7 cut(s) 1341, 1677, 1815, 1827, 2257, 2269, 2808
FspBI CTAG 6 cut(s) 1359, 1607, 2006, 2087, 2237, 2793
GlaI GCGC 3 cut(s) 1339, 2276, 3084
GluI GCNGC 7 cut(s) 1341, 1677, 1815, 1827, 2257, 2269, 2808
GsaI CCCAGC 2 cut(s) 245, 518
GsuI CTGGAG 4 cut(s) 537, 1199, 1857, 2898
HapII CCGG 9 cut(s) 161, 396, 540, 1044, 1389, 1449, 1466, 1854, 2545
HhaI GCGC 3 cut(s) 1340, 2277, 3085
Hin6I GCGC 3 cut(s) 1338, 2275, 3083
HinP1I GCGC 3 cut(s) 1338, 2275, 3083
HincII GTYRAC 5 cut(s) 1162, 1690, 1720, 2958, 3076
HindII GTYRAC 5 cut(s) 1162, 1690, 1720, 2958, 3076
HindIII AAGCTT 3 cut(s) 862, 1114, 2510
HpaI GTTAAC 2 cut(s) 1690, 1720
HpaII CCGG 9 cut(s) 161, 396, 540, 1044, 1389, 1449, 1466, 1854, 2545
HphI GGTGA 9 cut(s) 283, 385, 626, 634, 1141, 1438, 1805, 2165, 2823
Hpy99I CGWCG 2 cut(s) 1420, 1675
HpyCH4IV ACGT 2 cut(s) 1722, 3099
HpyF10VI GCNNNNNNNGC 9 cut(s) 89, 511, 1065, 1337, 1597, 1823, 2265, 2274, 3056
HpySE526I ACGT 2 cut(s) 1722, 3099
HspAI GCGC 3 cut(s) 1338, 2275, 3083
KflI GGGWCCC 1 cut(s) 226
Kpn2I TCCGGA 2 cut(s) 539, 1043
KpnI GGTACC 2 cut(s) 906, 1644
Ksp22I TGATCA 1 cut(s) 2314
KspAI GTTAAC 2 cut(s) 1690, 1720
Kzo9I GATC 9 cut(s) 100, 197, 379, 524, 949, 1499, 2314, 2646, 2683
LguI GCTCTTC 1 cut(s) 1877
LmnI GCTCC 5 cut(s) 484, 502, 828, 836, 1468
Lsp1109I GCAGC 6 cut(s) 1352, 1813, 1826, 2243, 2280, 2819
LweI GCATC 9 cut(s) 462, 528, 674, 2177, 2243, 2268, 2458, 2837, 3008
MaeI CTAG 6 cut(s) 1359, 1607, 2006, 2087, 2237, 2793
MaeII ACGT 2 cut(s) 1722, 3099
MalI GATC 9 cut(s) 102, 199, 381, 526, 951, 1501, 2316, 2648, 2685
MboI GATC 9 cut(s) 100, 197, 379, 524, 949, 1499, 2314, 2646, 2683
MfeI CAATTG 1 cut(s) 1698
MflI RGATCY 4 cut(s) 197, 949, 2646, 2683
MhlI GDGCHC 3 cut(s) 833, 2125, 2822
MlyI GAGTC 6 cut(s) 600, 1319, 2194, 2423, 2640, 2713
MroI TCCGGA 2 cut(s) 539, 1043
MroXI GAANNNNTTC 3 cut(s) 389, 749, 2001
MseI TTAA 9 cut(s) 875, 893, 1689, 1713, 1719, 1943, 2070, 2445, 2937
MslI CAYNNNNRTG 3 cut(s) 647, 2439, 2900
MspA1I CMGCKG 5 cut(s) 73, 600, 784, 1817, 2655
MspI CCGG 9 cut(s) 161, 396, 540, 1044, 1389, 1449, 1466, 1854, 2545
MspR9I CCNGG 8 cut(s) 162, 1200, 1466, 1497, 1855, 1875, 2545, 2546
MunI CAATTG 1 cut(s) 1698
Mva1269I GAATGC 1 cut(s) 410
MvaI CCWGG 3 cut(s) 1200, 1497, 1875
MwoI GCNNNNNNNGC 9 cut(s) 89, 511, 1065, 1337, 1597, 1823, 2265, 2274, 3056
NciI CCSGG 5 cut(s) 162, 1466, 1855, 2545, 2546
NdeII GATC 9 cut(s) 100, 197, 379, 524, 949, 1499, 2314, 2646, 2683
NmeAIII GCCGAG 1 cut(s) 2937
NmuCI GTSAC 5 cut(s) 1129, 1444, 1459, 2853, 3100
NspI RCATGY 2 cut(s) 285, 652
NspV TTCGAA 1 cut(s) 753
PaeR7I CTCGAG 1 cut(s) 578
PaqCI CACCTGC 1 cut(s) 1670
PciSI GCTCTTC 1 cut(s) 1877
PctI GAATGC 1 cut(s) 410
PdmI GAANNNNTTC 3 cut(s) 389, 749, 2001
PfeI GAWTC 4 cut(s) 807, 2233, 2364, 2756
PflFI GACNNNGTC 1 cut(s) 2953
PflMI CCANNNNNTGG 2 cut(s) 560, 2743
PfoI TCCNGGA 2 cut(s) 1198, 1873
PinAI ACCGGT 1 cut(s) 395
PkrI GCNGC 7 cut(s) 1342, 1678, 1816, 1828, 2258, 2270, 2809
PleI GAGTC 6 cut(s) 600, 1319, 2193, 2422, 2639, 2713
PpsI GAGTC 6 cut(s) 600, 1319, 2193, 2422, 2639, 2713
PpuMI RGGWCCY 2 cut(s) 226, 1411
PshBI ATTAAT 2 cut(s) 893, 1943
PsiI TTATAA 1 cut(s) 2115
Psp124BI GAGCTC 1 cut(s) 833
Psp5II RGGWCCY 2 cut(s) 226, 1411
Psp6I CCWGG 3 cut(s) 1198, 1495, 1873
PspEI GGTNACC 1 cut(s) 1129
PspFI CCCAGC 2 cut(s) 241, 514
PspGI CCWGG 3 cut(s) 1198, 1495, 1873
PspPPI RGGWCCY 2 cut(s) 226, 1411
PspXI VCTCGAGB 1 cut(s) 578
PstI CTGCAG 2 cut(s) 194, 2221
PstNI CAGNNNCTG 1 cut(s) 946
PsuI RGATCY 4 cut(s) 197, 949, 2646, 2683
PsyI GACNNNGTC 1 cut(s) 2953
PvuII CAGCTG 4 cut(s) 73, 600, 784, 1817
RsaI GTAC 6 cut(s) 5, 904, 1363, 1642, 2433, 3008
RsaNI GTAC 6 cut(s) 4, 903, 1362, 1641, 2432, 3007
RseI CAYNNNNRTG 3 cut(s) 647, 2439, 2900
SacI GAGCTC 1 cut(s) 833
SapI GCTCTTC 1 cut(s) 1877
SaqAI TTAA 9 cut(s) 875, 893, 1689, 1713, 1719, 1943, 2070, 2445, 2937
SatI GCNGC 7 cut(s) 1341, 1677, 1815, 1827, 2257, 2269, 2808
Sau3AI GATC 9 cut(s) 100, 197, 379, 524, 949, 1499, 2314, 2646, 2683
SchI GAGTC 6 cut(s) 600, 1319, 2194, 2423, 2640, 2713
ScrFI CCNGG 8 cut(s) 162, 1200, 1466, 1497, 1855, 1875, 2545, 2546
SduI GDGCHC 3 cut(s) 833, 2125, 2822
SfaNI GCATC 9 cut(s) 462, 528, 674, 2177, 2243, 2268, 2458, 2837, 3008
SfcI CTRYAG 4 cut(s) 190, 938, 1083, 2217
Sfr274I CTCGAG 1 cut(s) 578
SfuI TTCGAA 1 cut(s) 753
SinI GGWCC 5 cut(s) 226, 398, 470, 1046, 1411
SlaI CTCGAG 1 cut(s) 578
SmaI CCCGGG 1 cut(s) 2546
SmiMI CAYNNNNRTG 3 cut(s) 647, 2439, 2900
SmlI CTYRAG 4 cut(s) 578, 1000, 1076, 1526
SmoI CTYRAG 4 cut(s) 578, 1000, 1076, 1526
SpeI ACTAGT 1 cut(s) 1358
SsiI CCGC 5 cut(s) 1645, 1676, 1805, 2055, 2655
SspI AATATT 3 cut(s) 132, 1947, 2458
SspMI CTAG 6 cut(s) 1359, 1607, 2006, 2087, 2237, 2793
SstI GAGCTC 1 cut(s) 833
StyD4I CCNGG 8 cut(s) 160, 1198, 1464, 1495, 1853, 1873, 2543, 2544
StyI CCWWGG 3 cut(s) 480, 2176, 2423
TaiI ACGT 2 cut(s) 1725, 3102
TaqI TCGA 9 cut(s) 378, 579, 753, 1237, 2262, 2362, 2437, 2466, 2906
TatI WGTACW 4 cut(s) 3, 1361, 2431, 3006
TauI GCSGC 1 cut(s) 1679
TfiI GAWTC 4 cut(s) 807, 2233, 2364, 2756
Tru1I TTAA 9 cut(s) 875, 893, 1689, 1713, 1719, 1943, 2070, 2445, 2937
Tru9I TTAA 9 cut(s) 875, 893, 1689, 1713, 1719, 1943, 2070, 2445, 2937
TseFI GTSAC 5 cut(s) 1129, 1444, 1459, 2853, 3100
TseI GCWGC 6 cut(s) 1340, 1814, 1826, 2256, 2268, 2807
Tsp45I GTSAC 5 cut(s) 1129, 1444, 1459, 2853, 3100
TspGWI ACGGA 2 cut(s) 515, 1469
TspMI CCCGGG 1 cut(s) 2544
Tth111I GACNNNGTC 1 cut(s) 2953
Van91I CCANNNNNTGG 2 cut(s) 560, 2743
VneI GTGCAC 2 cut(s) 2121, 2818
VpaK11BI GGWCC 5 cut(s) 226, 398, 470, 1046, 1411
VspI ATTAAT 2 cut(s) 893, 1943
XagI CCTNNNNNAGG 2 cut(s) 2315, 2408
XapI RAATTY 7 cut(s) 341, 585, 618, 1222, 1709, 1743, 2739
XceI RCATGY 2 cut(s) 285, 652
XhoI CTCGAG 1 cut(s) 578
XmaI CCCGGG 1 cut(s) 2544
XmiI GTMKAC 1 cut(s) 937
XmnI GAANNNNTTC 3 cut(s) 389, 749, 2001
XspI CTAG 6 cut(s) 1359, 1607, 2006, 2087, 2237, 2793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.