Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1224 |
| AccB1I | GGYRCC | 2 cut(s) | 308, 1085 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 215 |
| AccI | GTMKAC | 1 cut(s) | 966 |
| AciI | CCGC | 3 cut(s) | 264, 645, 1241 |
| AclI | AACGTT | 1 cut(s) | 705 |
| AclWI | GGATC | 1 cut(s) | 173 |
| AcsI | RAATTY | 5 cut(s) | 145, 251, 406, 418, 598 |
| AcuI | CTGAAG | 1 cut(s) | 1195 |
| AfaI | GTAC | 1 cut(s) | 1136 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 215 |
| AgsI | TTSAA | 7 cut(s) | 145, 607, 710, 773, 945, 1000, 1081 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 321, 353 |
| Alw21I | GWGCWC | 1 cut(s) | 872 |
| Alw26I | GTCTC | 1 cut(s) | 198 |
| AlwI | GGATC | 1 cut(s) | 173 |
| AoxI | GGCC | 1 cut(s) | 174 |
| ApeKI | GCWGC | 3 cut(s) | 221, 443, 982 |
| ApoI | RAATTY | 5 cut(s) | 145, 251, 406, 418, 598 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 602 |
| AspS9I | GGNCC | 3 cut(s) | 174, 431, 791 |
| AsuHPI | GGTGA | 2 cut(s) | 388, 659 |
| AsuII | TTCGAA | 1 cut(s) | 786 |
| AvaII | GGWCC | 2 cut(s) | 431, 791 |
| BanI | GGYRCC | 2 cut(s) | 308, 1085 |
| BanII | GRGCYC | 1 cut(s) | 872 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 780, 812, 1167, 1199 |
| Bbv12I | GWGCWC | 1 cut(s) | 872 |
| BbvCI | CCTCAGC | 1 cut(s) | 920 |
| BbvI | GCAGC | 3 cut(s) | 208, 455, 994 |
| BccI | CCATC | 5 cut(s) | 28, 133, 592, 1061, 1142 |
| BciVI | GTATCC | 1 cut(s) | 717 |
| BcoDI | GTCTC | 1 cut(s) | 198 |
| BfaI | CTAG | 3 cut(s) | 593, 699, 1145 |
| BfmI | CTRYAG | 1 cut(s) | 441 |
| BfuAI | ACCTGC | 1 cut(s) | 1224 |
| BfuI | GTATCC | 1 cut(s) | 717 |
| BisI | GCNGC | 3 cut(s) | 222, 444, 983 |
| BlsI | GCNGC | 3 cut(s) | 223, 445, 984 |
| Bme18I | GGWCC | 2 cut(s) | 431, 791 |
| BmgT120I | GGNCC | 3 cut(s) | 174, 431, 791 |
| BmiI | GGNNCC | 3 cut(s) | 310, 1087, 1238 |
| BmsI | GCATC | 3 cut(s) | 54, 495, 1118 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 859, 891 |
| Bpu10I | CCTNAGC | 1 cut(s) | 920 |
| Bpu14I | TTCGAA | 1 cut(s) | 786 |
| BpuEI | CTTGAG | 4 cut(s) | 51, 84, 114, 784 |
| BsaI | GGTCTC | 1 cut(s) | 198 |
| BsaWI | WCCGGW | 1 cut(s) | 200 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 215 |
| Bse1I | ACTGG | 3 cut(s) | 237, 466, 1015 |
| Bse3DI | GCAATG | 2 cut(s) | 194, 447 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 215 |
| BseMI | GCAATG | 2 cut(s) | 194, 447 |
| BseMII | CTCAG | 4 cut(s) | 530, 579, 729, 911 |
| BseNI | ACTGG | 3 cut(s) | 237, 466, 1015 |
| BseRI | GAGGAG | 1 cut(s) | 81 |
| BseXI | GCAGC | 3 cut(s) | 208, 455, 994 |
| BshFI | GGCC | 1 cut(s) | 176 |
| BshNI | GGYRCC | 2 cut(s) | 308, 1085 |
| BsiHKAI | GWGCWC | 1 cut(s) | 872 |
| BsiSI | CCGG | 2 cut(s) | 201, 306 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 215 |
| BsmAI | GTCTC | 1 cut(s) | 198 |
| BsmI | GAATGC | 2 cut(s) | 862, 1155 |
| BsnI | GGCC | 1 cut(s) | 176 |
| Bso31I | GGTCTC | 1 cut(s) | 198 |
| Bsp119I | TTCGAA | 1 cut(s) | 786 |
| Bsp1286I | GDGCHC | 1 cut(s) | 872 |
| Bsp143I | GATC | 3 cut(s) | 178, 542, 670 |
| BspACI | CCGC | 3 cut(s) | 264, 645, 1241 |
| BspANI | GGCC | 1 cut(s) | 176 |
| BspCNI | CTCAG | 4 cut(s) | 531, 580, 728, 912 |
| BspLI | GGNNCC | 3 cut(s) | 310, 1087, 1238 |
| BspMAI | CTGCAG | 1 cut(s) | 445 |
| BspMI | ACCTGC | 1 cut(s) | 1224 |
| BspPI | GGATC | 1 cut(s) | 173 |
| BspQI | GCTCTTC | 1 cut(s) | 877 |
| BspT104I | TTCGAA | 1 cut(s) | 786 |
| BspT107I | GGYRCC | 2 cut(s) | 308, 1085 |
| BspTNI | GGTCTC | 1 cut(s) | 198 |
| BsrDI | GCAATG | 2 cut(s) | 194, 447 |
| BsrI | ACTGG | 3 cut(s) | 237, 466, 1015 |
| BssMI | GATC | 3 cut(s) | 178, 542, 670 |
| BssNAI | GTATAC | 1 cut(s) | 967 |
| Bst1107I | GTATAC | 1 cut(s) | 967 |
| Bst6I | CTCTTC | 2 cut(s) | 877, 1119 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 315, 449 |
| BstBI | TTCGAA | 1 cut(s) | 786 |
| BstC8I | GCNNGC | 1 cut(s) | 467 |
| BstDEI | CTNAG | 5 cut(s) | 539, 588, 715, 913, 920 |
| BstKTI | GATC | 3 cut(s) | 181, 545, 673 |
| BstMAI | GTCTC | 1 cut(s) | 198 |
| BstMBI | GATC | 3 cut(s) | 178, 542, 670 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 110, 315, 449, 589 |
| BstSFI | CTRYAG | 1 cut(s) | 441 |
| BstV1I | GCAGC | 3 cut(s) | 208, 455, 994 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 189 |
| BstZ17I | GTATAC | 1 cut(s) | 967 |
| BsuI | GTATCC | 1 cut(s) | 717 |
| BsuRI | GGCC | 1 cut(s) | 176 |
| BtsI | GCAGTG | 1 cut(s) | 220 |
| BtsIMutI | CAGTG | 3 cut(s) | 117, 156, 220 |
| BveI | ACCTGC | 1 cut(s) | 1224 |
| Cac8I | GCNNGC | 1 cut(s) | 467 |
| Cfr13I | GGNCC | 3 cut(s) | 174, 431, 791 |
| Csp6I | GTAC | 1 cut(s) | 1135 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 235, 270 |
| CviAII | CATG | 1 cut(s) | 210 |
| CviQI | GTAC | 1 cut(s) | 1135 |
| DdeI | CTNAG | 5 cut(s) | 539, 588, 715, 913, 920 |
| DpnI | GATC | 3 cut(s) | 180, 544, 672 |
| DpnII | GATC | 3 cut(s) | 178, 542, 670 |
| Eam1104I | CTCTTC | 2 cut(s) | 877, 1119 |
| EarI | CTCTTC | 2 cut(s) | 877, 1119 |
| Ecl136II | GAGCTC | 1 cut(s) | 870 |
| Eco24I | GRGCYC | 1 cut(s) | 872 |
| Eco31I | GGTCTC | 1 cut(s) | 198 |
| Eco47I | GGWCC | 2 cut(s) | 431, 791 |
| Eco53kI | GAGCTC | 1 cut(s) | 870 |
| Eco57I | CTGAAG | 1 cut(s) | 1195 |
| EcoICRI | GAGCTC | 1 cut(s) | 870 |
| EcoO109I | RGGNCCY | 2 cut(s) | 431, 791 |
| EcoRI | GAATTC | 2 cut(s) | 145, 418 |
| EcoT38I | GRGCYC | 1 cut(s) | 872 |
| FaeI | CATG | 1 cut(s) | 213 |
| FatI | CATG | 1 cut(s) | 209 |
| FauI | CCCGC | 2 cut(s) | 271, 1248 |
| FauNDI | CATATG | 1 cut(s) | 284 |
| FblI | GTMKAC | 1 cut(s) | 966 |
| Fnu4HI | GCNGC | 3 cut(s) | 222, 444, 983 |
| FriOI | GRGCYC | 1 cut(s) | 872 |
| Fsp4HI | GCNGC | 3 cut(s) | 222, 444, 983 |
| FspBI | CTAG | 3 cut(s) | 593, 699, 1145 |
| GluI | GCNGC | 3 cut(s) | 222, 444, 983 |
| HaeIII | GGCC | 1 cut(s) | 176 |
| HapII | CCGG | 2 cut(s) | 201, 306 |
| Hin1II | CATG | 1 cut(s) | 213 |
| HindIII | AAGCTT | 1 cut(s) | 752 |
| HinfI | GANTC | 5 cut(s) | 169, 230, 652, 941, 1025 |
| HpaII | CCGG | 2 cut(s) | 201, 306 |
| HphI | GGTGA | 2 cut(s) | 388, 659 |
| Hpy166II | GTNNAC | 2 cut(s) | 967, 1226 |
| Hpy188I | TCNGA | 6 cut(s) | 342, 405, 589, 819, 940, 955 |
| Hpy188III | TCNNGA | 3 cut(s) | 6, 368, 1145 |
| Hpy8I | GTNNAC | 2 cut(s) | 967, 1226 |
| HpyAV | CCTTC | 5 cut(s) | 782, 892, 960, 1100, 1170 |
| HpyCH4IV | ACGT | 2 cut(s) | 624, 705 |
| HpyCH4V | TGCA | 7 cut(s) | 45, 213, 443, 452, 469, 806, 881 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 110, 315, 449, 589 |
| HpyF3I | CTNAG | 5 cut(s) | 539, 588, 715, 913, 920 |
| HpySE526I | ACGT | 2 cut(s) | 624, 705 |
| Hsp92II | CATG | 1 cut(s) | 213 |
| Kzo9I | GATC | 3 cut(s) | 178, 542, 670 |
| LguI | GCTCTTC | 1 cut(s) | 877 |
| LmnI | GCTCC | 6 cut(s) | 82, 88, 94, 218, 867, 1152 |
| Lsp1109I | GCAGC | 3 cut(s) | 208, 455, 994 |
| LweI | GCATC | 3 cut(s) | 54, 495, 1118 |
| MaeI | CTAG | 3 cut(s) | 593, 699, 1145 |
| MaeII | ACGT | 2 cut(s) | 624, 705 |
| MaeIII | GTNAC | 2 cut(s) | 239, 287 |
| MalI | GATC | 3 cut(s) | 180, 544, 672 |
| MboI | GATC | 3 cut(s) | 178, 542, 670 |
| MboII | GAAGA | 4 cut(s) | 178, 864, 1106, 1187 |
| MhlI | GDGCHC | 1 cut(s) | 872 |
| MlyI | GAGTC | 2 cut(s) | 224, 1019 |
| MnlI | CCTC | 7 cut(s) | 25, 102, 108, 404, 444, 759, 915 |
| MroXI | GAANNNNTTC | 1 cut(s) | 602 |
| MseI | TTAA | 3 cut(s) | 1007, 1035, 1191 |
| MspA1I | CMGCKG | 1 cut(s) | 985 |
| MspI | CCGG | 2 cut(s) | 201, 306 |
| Mva1269I | GAATGC | 2 cut(s) | 862, 1155 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 110, 315, 449, 589 |
| NdeI | CATATG | 1 cut(s) | 284 |
| NdeII | GATC | 3 cut(s) | 178, 542, 670 |
| NlaIII | CATG | 1 cut(s) | 213 |
| NlaIV | GGNNCC | 3 cut(s) | 310, 1087, 1238 |
| NmuCI | GTSAC | 2 cut(s) | 239, 287 |
| NspV | TTCGAA | 1 cut(s) | 786 |
| PciSI | GCTCTTC | 1 cut(s) | 877 |
| PctI | GAATGC | 2 cut(s) | 862, 1155 |
| PdmI | GAANNNNTTC | 1 cut(s) | 602 |
| PfeI | GAWTC | 3 cut(s) | 169, 652, 941 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 215 |
| PkrI | GCNGC | 3 cut(s) | 223, 445, 984 |
| PleI | GAGTC | 2 cut(s) | 224, 1019 |
| PpsI | GAGTC | 2 cut(s) | 224, 1019 |
| PpuMI | RGGWCCY | 2 cut(s) | 431, 791 |
| Psp124BI | GAGCTC | 1 cut(s) | 872 |
| Psp1406I | AACGTT | 1 cut(s) | 705 |
| Psp5II | RGGWCCY | 2 cut(s) | 431, 791 |
| PspN4I | GGNNCC | 3 cut(s) | 310, 1087, 1238 |
| PspPI | GGNCC | 3 cut(s) | 174, 431, 791 |
| PspPPI | RGGWCCY | 2 cut(s) | 431, 791 |
| PstI | CTGCAG | 1 cut(s) | 445 |
| PvuII | CAGCTG | 1 cut(s) | 985 |
| RsaI | GTAC | 1 cut(s) | 1136 |
| RsaNI | GTAC | 1 cut(s) | 1135 |
| SacI | GAGCTC | 1 cut(s) | 872 |
| SapI | GCTCTTC | 1 cut(s) | 877 |
| SaqAI | TTAA | 3 cut(s) | 1007, 1035, 1191 |
| SatI | GCNGC | 3 cut(s) | 222, 444, 983 |
| Sau3AI | GATC | 3 cut(s) | 178, 542, 670 |
| Sau96I | GGNCC | 3 cut(s) | 174, 431, 791 |
| SchI | GAGTC | 2 cut(s) | 224, 1019 |
| SduI | GDGCHC | 1 cut(s) | 872 |
| SfaNI | GCATC | 3 cut(s) | 54, 495, 1118 |
| SfcI | CTRYAG | 1 cut(s) | 441 |
| SfuI | TTCGAA | 1 cut(s) | 786 |
| SinI | GGWCC | 2 cut(s) | 431, 791 |
| SmlI | CTYRAG | 4 cut(s) | 66, 99, 129, 763 |
| SmoI | CTYRAG | 4 cut(s) | 66, 99, 129, 763 |
| SsiI | CCGC | 3 cut(s) | 264, 645, 1241 |
| SspI | AATATT | 1 cut(s) | 934 |
| SspMI | CTAG | 3 cut(s) | 593, 699, 1145 |
| SstI | GAGCTC | 1 cut(s) | 872 |
| TaiI | ACGT | 2 cut(s) | 627, 708 |
| TaqI | TCGA | 2 cut(s) | 786, 854 |
| TfiI | GAWTC | 3 cut(s) | 169, 652, 941 |
| Tru1I | TTAA | 3 cut(s) | 1007, 1035, 1191 |
| Tru9I | TTAA | 3 cut(s) | 1007, 1035, 1191 |
| TscAI | CASTG | 3 cut(s) | 124, 156, 220 |
| TseFI | GTSAC | 2 cut(s) | 239, 287 |
| TseI | GCWGC | 3 cut(s) | 221, 443, 982 |
| Tsp45I | GTSAC | 2 cut(s) | 239, 287 |
| TspDTI | ATGAA | 2 cut(s) | 842, 911 |
| TspRI | CASTG | 3 cut(s) | 124, 156, 220 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 215 |
| VpaK11BI | GGWCC | 2 cut(s) | 431, 791 |
| XapI | RAATTY | 5 cut(s) | 145, 251, 406, 418, 598 |
| XbaI | TCTAGA | 1 cut(s) | 1144 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 375, 406 |
| XmiI | GTMKAC | 1 cut(s) | 966 |
| XmnI | GAANNNNTTC | 1 cut(s) | 602 |
| XspI | CTAG | 3 cut(s) | 593, 699, 1145 |