Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 500 |
| AccBSI | CCGCTC | 1 cut(s) | 486 |
| AccII | CGCG | 1 cut(s) | 137 |
| AccIII | TCCGGA | 2 cut(s) | 72, 559 |
| AciI | CCGC | 8 cut(s) | 5, 22, 135, 191, 284, 414, 456, 484 |
| AclWI | GGATC | 2 cut(s) | 210, 446 |
| AcoI | YGGCCR | 2 cut(s) | 19, 57 |
| AcsI | RAATTY | 2 cut(s) | 337, 443 |
| AcyI | GRCGYC | 2 cut(s) | 162, 211 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 150, 407 |
| AgsI | TTSAA | 1 cut(s) | 337 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 513, 545 |
| AluBI | AGCT | 3 cut(s) | 40, 244, 258 |
| AluI | AGCT | 3 cut(s) | 40, 244, 258 |
| Alw21I | GWGCWC | 1 cut(s) | 42 |
| Alw26I | GTCTC | 2 cut(s) | 101, 390 |
| AlwI | GGATC | 2 cut(s) | 210, 446 |
| Aor13HI | TCCGGA | 2 cut(s) | 72, 559 |
| AoxI | GGCC | 5 cut(s) | 6, 19, 57, 112, 366 |
| ApoI | RAATTY | 2 cut(s) | 337, 443 |
| AsiSI | GCGATCGC | 1 cut(s) | 141 |
| AspS9I | GGNCC | 3 cut(s) | 70, 75, 366 |
| AsuC2I | CCSGG | 2 cut(s) | 30, 151 |
| AvaII | GGWCC | 2 cut(s) | 70, 75 |
| BanI | GGYRCC | 1 cut(s) | 500 |
| BanII | GRGCYC | 1 cut(s) | 42 |
| BbsI | GAAGAC | 1 cut(s) | 79 |
| Bbv12I | GWGCWC | 1 cut(s) | 42 |
| BccI | CCATC | 1 cut(s) | 325 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 32, 66, 70, 104 |
| BcnI | CCSGG | 2 cut(s) | 30, 151 |
| BcoDI | GTCTC | 2 cut(s) | 101, 390 |
| BfaI | CTAG | 1 cut(s) | 186 |
| BisI | GCNGC | 4 cut(s) | 6, 22, 192, 414 |
| BlsI | GCNGC | 4 cut(s) | 7, 23, 193, 415 |
| Bme1390I | CCNGG | 2 cut(s) | 30, 151 |
| Bme18I | GGWCC | 2 cut(s) | 70, 75 |
| BmgT120I | GGNCC | 3 cut(s) | 70, 75, 366 |
| BmiI | GGNNCC | 2 cut(s) | 412, 502 |
| BmrFI | CCNGG | 2 cut(s) | 30, 151 |
| BmsI | GCATC | 1 cut(s) | 90 |
| BpiI | GAAGAC | 1 cut(s) | 79 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 24, 56 |
| BpuEI | CTTGAG | 3 cut(s) | 244, 276, 306 |
| BpuMI | CCSGG | 2 cut(s) | 30, 151 |
| BsaHI | GRCGYC | 2 cut(s) | 162, 211 |
| BsaI | GGTCTC | 1 cut(s) | 390 |
| BsaJI | CCNNGG | 1 cut(s) | 9 |
| BsaWI | WCCGGW | 3 cut(s) | 72, 392, 559 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 150, 407 |
| Bse118I | RCCGGY | 1 cut(s) | 77 |
| Bse3DI | GCAATG | 1 cut(s) | 386 |
| BseAI | TCCGGA | 2 cut(s) | 72, 559 |
| BseDI | CCNNGG | 1 cut(s) | 9 |
| BseGI | GGATG | 1 cut(s) | 165 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 150, 407 |
| BseMI | GCAATG | 1 cut(s) | 386 |
| BseX3I | CGGCCG | 1 cut(s) | 19 |
| Bsh1236I | CGCG | 1 cut(s) | 137 |
| Bsh1285I | CGRYCG | 3 cut(s) | 22, 141, 312 |
| BshFI | GGCC | 5 cut(s) | 8, 21, 59, 114, 368 |
| BshNI | GGYRCC | 1 cut(s) | 500 |
| BsiEI | CGRYCG | 3 cut(s) | 22, 141, 312 |
| BsiHKAI | GWGCWC | 1 cut(s) | 42 |
| BsiSI | CCGG | 9 cut(s) | 18, 30, 56, 73, 78, 151, 393, 498, 560 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 150, 407 |
| BsmAI | GTCTC | 2 cut(s) | 101, 390 |
| BsmBI | CGTCTC | 1 cut(s) | 101 |
| BsnI | GGCC | 5 cut(s) | 8, 21, 59, 114, 368 |
| Bso31I | GGTCTC | 1 cut(s) | 390 |
| Bsp1286I | GDGCHC | 1 cut(s) | 42 |
| Bsp13I | TCCGGA | 2 cut(s) | 72, 559 |
| Bsp143I | GATC | 4 cut(s) | 108, 138, 215, 451 |
| BspACI | CCGC | 8 cut(s) | 5, 22, 135, 191, 284, 414, 456, 484 |
| BspANI | GGCC | 5 cut(s) | 8, 21, 59, 114, 368 |
| BspEI | TCCGGA | 2 cut(s) | 72, 559 |
| BspFNI | CGCG | 1 cut(s) | 137 |
| BspLI | GGNNCC | 2 cut(s) | 412, 502 |
| BspPI | GGATC | 2 cut(s) | 210, 446 |
| BspT107I | GGYRCC | 1 cut(s) | 500 |
| BspTNI | GGTCTC | 1 cut(s) | 390 |
| BsrBI | CCGCTC | 1 cut(s) | 486 |
| BsrDI | GCAATG | 1 cut(s) | 386 |
| BsrFI | RCCGGY | 1 cut(s) | 77 |
| BssAI | RCCGGY | 1 cut(s) | 77 |
| BssECI | CCNNGG | 1 cut(s) | 9 |
| BssMI | GATC | 4 cut(s) | 108, 138, 215, 451 |
| BssNI | GRCGYC | 2 cut(s) | 162, 211 |
| BstACI | GRCGYC | 2 cut(s) | 162, 211 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 507 |
| BstC8I | GCNNGC | 1 cut(s) | 51 |
| BstF5I | GGATG | 1 cut(s) | 165 |
| BstFNI | CGCG | 1 cut(s) | 137 |
| BstKTI | GATC | 4 cut(s) | 111, 141, 218, 454 |
| BstMAI | GTCTC | 2 cut(s) | 101, 390 |
| BstMBI | GATC | 4 cut(s) | 108, 138, 215, 451 |
| BstMCI | CGRYCG | 3 cut(s) | 22, 141, 312 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 11, 46, 302, 507 |
| BstSCI | CCNGG | 2 cut(s) | 28, 149 |
| BstUI | CGCG | 1 cut(s) | 137 |
| BstV2I | GAAGAC | 1 cut(s) | 79 |
| BstZI | CGGCCG | 1 cut(s) | 19 |
| BsuRI | GGCC | 5 cut(s) | 8, 21, 59, 114, 368 |
| BtsCI | GGATG | 1 cut(s) | 165 |
| BtsI | GCAGTG | 1 cut(s) | 412 |
| BtsIMutI | CAGTG | 1 cut(s) | 412 |
| Cac8I | GCNNGC | 1 cut(s) | 51 |
| Cfr10I | RCCGGY | 1 cut(s) | 77 |
| Cfr13I | GGNCC | 3 cut(s) | 70, 75, 366 |
| CpoI | CGGWCCG | 2 cut(s) | 70, 75 |
| CseI | GACGC | 2 cut(s) | 151, 200 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 427, 462 |
| CspI | CGGWCCG | 2 cut(s) | 70, 75 |
| CviAII | CATG | 1 cut(s) | 475 |
| DpnI | GATC | 4 cut(s) | 110, 140, 217, 453 |
| DpnII | GATC | 4 cut(s) | 108, 138, 215, 451 |
| EaeI | YGGCCR | 2 cut(s) | 19, 57 |
| EagI | CGGCCG | 1 cut(s) | 19 |
| Ecl136II | GAGCTC | 1 cut(s) | 40 |
| EclXI | CGGCCG | 1 cut(s) | 19 |
| Eco24I | GRGCYC | 1 cut(s) | 42 |
| Eco31I | GGTCTC | 1 cut(s) | 390 |
| Eco47I | GGWCC | 2 cut(s) | 70, 75 |
| Eco52I | CGGCCG | 1 cut(s) | 19 |
| Eco53kI | GAGCTC | 1 cut(s) | 40 |
| EcoICRI | GAGCTC | 1 cut(s) | 40 |
| EcoRI | GAATTC | 1 cut(s) | 337 |
| EcoT38I | GRGCYC | 1 cut(s) | 42 |
| Esp3I | CGTCTC | 1 cut(s) | 101 |
| FaeI | CATG | 1 cut(s) | 478 |
| FaiI | YATR | 3 cut(s) | 237, 419, 476 |
| FatI | CATG | 1 cut(s) | 474 |
| FauI | CCCGC | 1 cut(s) | 463 |
| Fnu4HI | GCNGC | 4 cut(s) | 6, 22, 192, 414 |
| FokI | GGATG | 1 cut(s) | 152 |
| FriOI | GRGCYC | 1 cut(s) | 42 |
| Fsp4HI | GCNGC | 4 cut(s) | 6, 22, 192, 414 |
| FspBI | CTAG | 1 cut(s) | 186 |
| GluI | GCNGC | 4 cut(s) | 6, 22, 192, 414 |
| HaeIII | GGCC | 5 cut(s) | 8, 21, 59, 114, 368 |
| HapII | CCGG | 9 cut(s) | 18, 30, 56, 73, 78, 151, 393, 498, 560 |
| HgaI | GACGC | 2 cut(s) | 151, 200 |
| Hin1I | GRCGYC | 2 cut(s) | 162, 211 |
| Hin1II | CATG | 1 cut(s) | 478 |
| HinfI | GANTC | 5 cut(s) | 33, 44, 146, 264, 422 |
| HpaII | CCGG | 9 cut(s) | 18, 30, 56, 73, 78, 151, 393, 498, 560 |
| Hpy166II | GTNNAC | 1 cut(s) | 250 |
| Hpy188I | TCNGA | 5 cut(s) | 101, 108, 220, 375, 534 |
| Hpy188III | TCNNGA | 3 cut(s) | 73, 261, 560 |
| Hpy8I | GTNNAC | 1 cut(s) | 250 |
| Hpy99I | CGWCG | 4 cut(s) | 127, 177, 180, 216 |
| HpyAV | CCTTC | 2 cut(s) | 125, 478 |
| HpyCH4V | TGCA | 1 cut(s) | 405 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 11, 46, 302, 507 |
| Hsp92I | GRCGYC | 2 cut(s) | 162, 211 |
| Hsp92II | CATG | 1 cut(s) | 478 |
| Kpn2I | TCCGGA | 2 cut(s) | 72, 559 |
| Kzo9I | GATC | 4 cut(s) | 108, 138, 215, 451 |
| LmnI | GCTCC | 5 cut(s) | 58, 241, 275, 286, 410 |
| LpnPI | CCDG | 8 cut(s) | 31, 43, 69, 86, 91, 164, 406, 511 |
| LweI | GCATC | 1 cut(s) | 90 |
| MaeI | CTAG | 1 cut(s) | 186 |
| MaeIII | GTNAC | 1 cut(s) | 479 |
| MalI | GATC | 4 cut(s) | 110, 140, 217, 453 |
| MbiI | CCGCTC | 1 cut(s) | 486 |
| MboI | GATC | 4 cut(s) | 108, 138, 215, 451 |
| MboII | GAAGA | 2 cut(s) | 79, 174 |
| MhlI | GDGCHC | 1 cut(s) | 42 |
| MluCI | AATT | 3 cut(s) | 337, 443, 555 |
| MlyI | GAGTC | 2 cut(s) | 42, 416 |
| MnlI | CCTC | 3 cut(s) | 19, 179, 300 |
| MroI | TCCGGA | 2 cut(s) | 72, 559 |
| MspI | CCGG | 9 cut(s) | 18, 30, 56, 73, 78, 151, 393, 498, 560 |
| MspR9I | CCNGG | 2 cut(s) | 30, 151 |
| MvnI | CGCG | 1 cut(s) | 137 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 11, 46, 302, 507 |
| NciI | CCSGG | 2 cut(s) | 30, 151 |
| NdeII | GATC | 4 cut(s) | 108, 138, 215, 451 |
| NlaIII | CATG | 1 cut(s) | 478 |
| NlaIV | GGNNCC | 2 cut(s) | 412, 502 |
| NmuCI | GTSAC | 1 cut(s) | 479 |
| PfeI | GAWTC | 3 cut(s) | 44, 146, 264 |
| PkrI | GCNGC | 4 cut(s) | 7, 23, 193, 415 |
| Ple19I | CGATCG | 1 cut(s) | 141 |
| PleI | GAGTC | 2 cut(s) | 41, 416 |
| PpsI | GAGTC | 2 cut(s) | 41, 416 |
| Psp124BI | GAGCTC | 1 cut(s) | 42 |
| PspN4I | GGNNCC | 2 cut(s) | 412, 502 |
| PspPI | GGNCC | 3 cut(s) | 70, 75, 366 |
| PvuI | CGATCG | 1 cut(s) | 141 |
| RgaI | GCGATCGC | 1 cut(s) | 141 |
| Rsr2I | CGGWCCG | 2 cut(s) | 70, 75 |
| RsrII | CGGWCCG | 2 cut(s) | 70, 75 |
| SacI | GAGCTC | 1 cut(s) | 42 |
| SatI | GCNGC | 4 cut(s) | 6, 22, 192, 414 |
| Sau3AI | GATC | 4 cut(s) | 108, 138, 215, 451 |
| Sau96I | GGNCC | 3 cut(s) | 70, 75, 366 |
| SchI | GAGTC | 2 cut(s) | 42, 416 |
| ScrFI | CCNGG | 2 cut(s) | 30, 151 |
| SduI | GDGCHC | 1 cut(s) | 42 |
| SetI | ASST | 4 cut(s) | 42, 246, 260, 292 |
| SfaAI | GCGATCGC | 1 cut(s) | 141 |
| SfaNI | GCATC | 1 cut(s) | 90 |
| SgfI | GCGATCGC | 1 cut(s) | 141 |
| SinI | GGWCC | 2 cut(s) | 70, 75 |
| SmlI | CTYRAG | 3 cut(s) | 259, 291, 321 |
| SmoI | CTYRAG | 3 cut(s) | 259, 291, 321 |
| Sse9I | AATT | 3 cut(s) | 337, 443, 555 |
| SsiI | CCGC | 8 cut(s) | 5, 22, 135, 191, 284, 414, 456, 484 |
| SspI | AATATT | 1 cut(s) | 361 |
| SspMI | CTAG | 1 cut(s) | 186 |
| SstI | GAGCTC | 1 cut(s) | 42 |
| StyD4I | CCNGG | 2 cut(s) | 28, 149 |
| TaqI | TCGA | 3 cut(s) | 36, 42, 214 |
| TaqII | GACCGA | 2 cut(s) | 58, 326 |
| TasI | AATT | 3 cut(s) | 337, 443, 555 |
| TauI | GCSGC | 4 cut(s) | 8, 24, 194, 416 |
| TfiI | GAWTC | 3 cut(s) | 44, 146, 264 |
| TscAI | CASTG | 1 cut(s) | 412 |
| TseFI | GTSAC | 1 cut(s) | 479 |
| Tsp45I | GTSAC | 1 cut(s) | 479 |
| TspDTI | ATGAA | 1 cut(s) | 460 |
| TspGWI | ACGGA | 3 cut(s) | 83, 111, 161 |
| TspRI | CASTG | 1 cut(s) | 412 |
| VpaK11BI | GGWCC | 2 cut(s) | 70, 75 |
| XapI | RAATTY | 2 cut(s) | 337, 443 |
| XspI | CTAG | 1 cut(s) | 186 |