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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1043 |
| AatII | GACGTC | 1 cut(s) | 33 |
| Acc16I | TGCGCA | 1 cut(s) | 206 |
| Acc65I | GGTACC | 1 cut(s) | 47 |
| AccB1I | GGYRCC | 4 cut(s) | 47, 593, 773, 794 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 756 |
| AccBSI | CCGCTC | 1 cut(s) | 800 |
| AccIII | TCCGGA | 1 cut(s) | 607 |
| AciI | CCGC | 7 cut(s) | 140, 519, 631, 777, 792, 798, 915 |
| AclWI | GGATC | 6 cut(s) | 108, 119, 188, 224, 457, 666 |
| AcoI | YGGCCR | 1 cut(s) | 742 |
| AcsI | RAATTY | 2 cut(s) | 23, 603 |
| AcuI | CTGAAG | 1 cut(s) | 435 |
| AcvI | CACGTG | 1 cut(s) | 686 |
| AcyI | GRCGYC | 2 cut(s) | 30, 760 |
| AfaI | GTAC | 2 cut(s) | 49, 56 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 64, 328, 756, 791 |
| AgeI | ACCGGT | 1 cut(s) | 770 |
| AgsI | TTSAA | 3 cut(s) | 23, 974, 991 |
| AleI | CACNNNNGTG | 1 cut(s) | 702 |
| AluBI | AGCT | 6 cut(s) | 131, 380, 390, 455, 644, 888 |
| AluI | AGCT | 6 cut(s) | 131, 380, 390, 455, 644, 888 |
| Alw21I | GWGCWC | 1 cut(s) | 392 |
| Alw26I | GTCTC | 3 cut(s) | 73, 104, 759 |
| AlwI | GGATC | 6 cut(s) | 108, 119, 188, 224, 457, 666 |
| Ama87I | CYCGRG | 1 cut(s) | 717 |
| Aor13HI | TCCGGA | 1 cut(s) | 607 |
| AoxI | GGCC | 2 cut(s) | 742, 781 |
| ApeKI | GCWGC | 2 cut(s) | 455, 814 |
| ApoI | RAATTY | 2 cut(s) | 23, 603 |
| AsiGI | ACCGGT | 1 cut(s) | 770 |
| Asp718I | GGTACC | 1 cut(s) | 47 |
| AspLEI | GCGC | 2 cut(s) | 169, 207 |
| AspS9I | GGNCC | 4 cut(s) | 105, 782, 864, 871 |
| AsuHPI | GGTGA | 1 cut(s) | 1039 |
| AvaI | CYCGRG | 1 cut(s) | 717 |
| AvaII | GGWCC | 3 cut(s) | 105, 864, 871 |
| BalI | TGGCCA | 1 cut(s) | 744 |
| BanI | GGYRCC | 4 cut(s) | 47, 593, 773, 794 |
| BanII | GRGCYC | 1 cut(s) | 392 |
| BbrPI | CACGTG | 1 cut(s) | 686 |
| Bbv12I | GWGCWC | 1 cut(s) | 392 |
| BbvI | GCAGC | 2 cut(s) | 442, 826 |
| BccI | CCATC | 2 cut(s) | 66, 934 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 7, 41, 803, 837 |
| BclI | TGATCA | 2 cut(s) | 561, 568 |
| BcoDI | GTCTC | 3 cut(s) | 73, 104, 759 |
| BfoI | RGCGCY | 1 cut(s) | 170 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 789 |
| BglII | AGATCT | 1 cut(s) | 238 |
| BisI | GCNGC | 6 cut(s) | 456, 520, 777, 798, 815, 915 |
| BlsI | GCNGC | 6 cut(s) | 457, 521, 778, 799, 816, 916 |
| Bme18I | GGWCC | 3 cut(s) | 105, 864, 871 |
| BmeT110I | CYCGRG | 1 cut(s) | 717 |
| BmgT120I | GGNCC | 4 cut(s) | 105, 782, 864, 871 |
| BmiI | GGNNCC | 7 cut(s) | 49, 138, 595, 775, 784, 796, 865 |
| BmsI | GCATC | 3 cut(s) | 238, 403, 1018 |
| Bpu10I | CCTNAGC | 2 cut(s) | 96, 132 |
| BpuEI | CTTGAG | 1 cut(s) | 506 |
| Bsa29I | ATCGAT | 1 cut(s) | 192 |
| BsaAI | YACGTR | 1 cut(s) | 686 |
| BsaBI | GATNNNNATC | 1 cut(s) | 663 |
| BsaHI | GRCGYC | 2 cut(s) | 30, 760 |
| BsaI | GGTCTC | 2 cut(s) | 73, 104 |
| BsaJI | CCNNGG | 4 cut(s) | 43, 786, 806, 867 |
| BsaWI | WCCGGW | 2 cut(s) | 607, 770 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 64, 328, 756, 791 |
| Bse118I | RCCGGY | 3 cut(s) | 770, 779, 1049 |
| Bse1I | ACTGG | 1 cut(s) | 881 |
| Bse8I | GATNNNNATC | 1 cut(s) | 663 |
| BseAI | TCCGGA | 1 cut(s) | 607 |
| BseCI | ATCGAT | 1 cut(s) | 192 |
| BseDI | CCNNGG | 4 cut(s) | 43, 786, 806, 867 |
| BseGI | GGATG | 2 cut(s) | 253, 319 |
| BseJI | GATNNNNATC | 1 cut(s) | 663 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 64, 328, 756, 791 |
| BseMII | CTCAG | 4 cut(s) | 146, 176, 377, 707 |
| BseNI | ACTGG | 1 cut(s) | 881 |
| BseRI | GAGGAG | 2 cut(s) | 792, 795 |
| BseXI | GCAGC | 2 cut(s) | 442, 826 |
| BseYI | CCCAGC | 2 cut(s) | 63, 309 |
| BshFI | GGCC | 2 cut(s) | 744, 783 |
| BshNI | GGYRCC | 4 cut(s) | 47, 593, 773, 794 |
| BshTI | ACCGGT | 1 cut(s) | 770 |
| BshVI | ATCGAT | 1 cut(s) | 192 |
| BsiHKAI | GWGCWC | 1 cut(s) | 392 |
| BsiHKCI | CYCGRG | 1 cut(s) | 717 |
| BsiSI | CCGG | 4 cut(s) | 608, 771, 780, 1050 |
| BslFI | GGGAC | 2 cut(s) | 703, 735 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 64, 328, 756, 791 |
| BsmAI | GTCTC | 3 cut(s) | 73, 104, 759 |
| BsmFI | GGGAC | 2 cut(s) | 703, 735 |
| BsnI | GGCC | 2 cut(s) | 744, 783 |
| Bso31I | GGTCTC | 2 cut(s) | 73, 104 |
| BsoBI | CYCGRG | 1 cut(s) | 717 |
| Bsp1286I | GDGCHC | 1 cut(s) | 392 |
| Bsp13I | TCCGGA | 1 cut(s) | 607 |
| Bsp19I | CCATGG | 1 cut(s) | 43 |
| BspACI | CCGC | 7 cut(s) | 140, 519, 631, 777, 792, 798, 915 |
| BspANI | GGCC | 2 cut(s) | 744, 783 |
| BspCNI | CTCAG | 4 cut(s) | 145, 175, 378, 706 |
| BspDI | ATCGAT | 1 cut(s) | 192 |
| BspEI | TCCGGA | 1 cut(s) | 607 |
| BspHI | TCATGA | 1 cut(s) | 468 |
| BspLI | GGNNCC | 7 cut(s) | 49, 138, 595, 775, 784, 796, 865 |
| BspPI | GGATC | 6 cut(s) | 108, 119, 188, 224, 457, 666 |
| BspQI | GCTCTTC | 1 cut(s) | 832 |
| BspT107I | GGYRCC | 4 cut(s) | 47, 593, 773, 794 |
| BspTNI | GGTCTC | 2 cut(s) | 73, 104 |
| BsrBI | CCGCTC | 1 cut(s) | 800 |
| BsrFI | RCCGGY | 3 cut(s) | 770, 779, 1049 |
| BsrI | ACTGG | 1 cut(s) | 881 |
| BssAI | RCCGGY | 3 cut(s) | 770, 779, 1049 |
| BssECI | CCNNGG | 4 cut(s) | 43, 786, 806, 867 |
| BssNI | GRCGYC | 2 cut(s) | 30, 760 |
| BssT1I | CCWWGG | 3 cut(s) | 43, 786, 867 |
| Bst4CI | ACNGT | 2 cut(s) | 704, 845 |
| Bst6I | CTCTTC | 1 cut(s) | 832 |
| BstACI | GRCGYC | 2 cut(s) | 30, 760 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 580 |
| BstBAI | YACGTR | 1 cut(s) | 686 |
| BstC8I | GCNNGC | 4 cut(s) | 209, 307, 629, 781 |
| BstDEI | CTNAG | 8 cut(s) | 96, 132, 162, 386, 459, 498, 693, 1024 |
| BstDSI | CCRYGG | 1 cut(s) | 43 |
| BstF5I | GGATG | 2 cut(s) | 253, 319 |
| BstH2I | RGCGCY | 1 cut(s) | 170 |
| BstHHI | GCGC | 2 cut(s) | 169, 207 |
| BstMAI | GTCTC | 3 cut(s) | 73, 104, 759 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 580, 741, 789 |
| BstV1I | GCAGC | 2 cut(s) | 442, 826 |
| BstX2I | RGATCY | 2 cut(s) | 238, 462 |
| BstYI | RGATCY | 2 cut(s) | 238, 462 |
| Bsu15I | ATCGAT | 1 cut(s) | 192 |
| BsuRI | GGCC | 2 cut(s) | 744, 783 |
| BsuTUI | ATCGAT | 1 cut(s) | 192 |
| BtgI | CCRYGG | 1 cut(s) | 43 |
| BtgZI | GCGATG | 2 cut(s) | 44, 76 |
| BtsCI | GGATG | 2 cut(s) | 253, 319 |
| BtsI | GCAGTG | 1 cut(s) | 200 |
| BtsIMutI | CAGTG | 3 cut(s) | 200, 874, 954 |
| Cac8I | GCNNGC | 4 cut(s) | 209, 307, 629, 781 |
| CciI | TCATGA | 1 cut(s) | 468 |
| CfoI | GCGC | 2 cut(s) | 169, 207 |
| Cfr10I | RCCGGY | 3 cut(s) | 770, 779, 1049 |
| Cfr13I | GGNCC | 4 cut(s) | 105, 782, 864, 871 |
| ClaI | ATCGAT | 1 cut(s) | 192 |
| CpoI | CGGWCCG | 1 cut(s) | 105 |
| CseI | GACGC | 3 cut(s) | 538, 572, 749 |
| Csp6I | GTAC | 2 cut(s) | 48, 55 |
| CspAI | ACCGGT | 1 cut(s) | 770 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 841, 876 |
| CspI | CGGWCCG | 1 cut(s) | 105 |
| CviAII | CATG | 6 cut(s) | 44, 172, 393, 421, 469, 559 |
| CviQI | GTAC | 2 cut(s) | 48, 55 |
| DdeI | CTNAG | 8 cut(s) | 96, 132, 162, 386, 459, 498, 693, 1024 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1043 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1043 |
| EaeI | YGGCCR | 1 cut(s) | 742 |
| Eam1104I | CTCTTC | 1 cut(s) | 832 |
| EarI | CTCTTC | 1 cut(s) | 832 |
| Ecl136II | GAGCTC | 1 cut(s) | 390 |
| Eco130I | CCWWGG | 3 cut(s) | 43, 786, 867 |
| Eco24I | GRGCYC | 1 cut(s) | 392 |
| Eco31I | GGTCTC | 2 cut(s) | 73, 104 |
| Eco47I | GGWCC | 3 cut(s) | 105, 864, 871 |
| Eco53kI | GAGCTC | 1 cut(s) | 390 |
| Eco57I | CTGAAG | 1 cut(s) | 435 |
| Eco72I | CACGTG | 1 cut(s) | 686 |
| Eco88I | CYCGRG | 1 cut(s) | 717 |
| EcoICRI | GAGCTC | 1 cut(s) | 390 |
| EcoRI | GAATTC | 1 cut(s) | 23 |
| EcoT14I | CCWWGG | 3 cut(s) | 43, 786, 867 |
| EcoT38I | GRGCYC | 1 cut(s) | 392 |
| ErhI | CCWWGG | 3 cut(s) | 43, 786, 867 |
| FaeI | CATG | 6 cut(s) | 47, 175, 396, 424, 472, 562 |
| FaqI | GGGAC | 2 cut(s) | 703, 735 |
| FatI | CATG | 6 cut(s) | 43, 171, 392, 420, 468, 558 |
| FbaI | TGATCA | 2 cut(s) | 561, 568 |
| Fnu4HI | GCNGC | 6 cut(s) | 456, 520, 777, 798, 815, 915 |
| FokI | GGATG | 2 cut(s) | 260, 326 |
| FriOI | GRGCYC | 1 cut(s) | 392 |
| Fsp4HI | GCNGC | 6 cut(s) | 456, 520, 777, 798, 815, 915 |
| FspI | TGCGCA | 1 cut(s) | 206 |
| GlaI | GCGC | 2 cut(s) | 168, 206 |
| GluI | GCNGC | 6 cut(s) | 456, 520, 777, 798, 815, 915 |
| GsaI | CCCAGC | 2 cut(s) | 67, 313 |
| HaeII | RGCGCY | 1 cut(s) | 170 |
| HaeIII | GGCC | 2 cut(s) | 744, 783 |
| HapII | CCGG | 4 cut(s) | 608, 771, 780, 1050 |
| HgaI | GACGC | 3 cut(s) | 538, 572, 749 |
| HhaI | GCGC | 2 cut(s) | 169, 207 |
| Hin1I | GRCGYC | 2 cut(s) | 30, 760 |
| Hin1II | CATG | 6 cut(s) | 47, 175, 396, 424, 472, 562 |
| Hin6I | GCGC | 2 cut(s) | 167, 205 |
| HinP1I | GCGC | 2 cut(s) | 167, 205 |
| HincII | GTYRAC | 1 cut(s) | 848 |
| HindII | GTYRAC | 1 cut(s) | 848 |
| HinfI | GANTC | 4 cut(s) | 399, 611, 634, 691 |
| HpaII | CCGG | 4 cut(s) | 608, 771, 780, 1050 |
| HphI | GGTGA | 1 cut(s) | 1039 |
| Hpy166II | GTNNAC | 2 cut(s) | 492, 848 |
| Hpy188I | TCNGA | 7 cut(s) | 73, 105, 323, 648, 669, 810, 1021 |
| Hpy188III | TCNNGA | 6 cut(s) | 134, 403, 439, 469, 608, 764 |
| Hpy8I | GTNNAC | 2 cut(s) | 492, 848 |
| Hpy99I | CGWCG | 2 cut(s) | 32, 765 |
| HpyAV | CCTTC | 6 cut(s) | 97, 188, 334, 454, 459, 997 |
| HpyCH4III | ACNGT | 2 cut(s) | 704, 845 |
| HpyCH4IV | ACGT | 2 cut(s) | 30, 685 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 683, 902 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 580, 741, 789 |
| HpyF3I | CTNAG | 8 cut(s) | 96, 132, 162, 386, 459, 498, 693, 1024 |
| HpySE526I | ACGT | 2 cut(s) | 30, 685 |
| Hsp92I | GRCGYC | 2 cut(s) | 30, 760 |
| Hsp92II | CATG | 6 cut(s) | 47, 175, 396, 424, 472, 562 |
| HspAI | GCGC | 2 cut(s) | 167, 205 |
| Kpn2I | TCCGGA | 1 cut(s) | 607 |
| KpnI | GGTACC | 1 cut(s) | 51 |
| KroI | GCCGGC | 1 cut(s) | 779 |
| KroNI | GCCGGC | 1 cut(s) | 781 |
| Ksp22I | TGATCA | 2 cut(s) | 561, 568 |
| LguI | GCTCTTC | 1 cut(s) | 832 |
| LmnI | GCTCC | 3 cut(s) | 294, 649, 805 |
| Lsp1109I | GCAGC | 2 cut(s) | 442, 826 |
| LweI | GCATC | 3 cut(s) | 238, 403, 1018 |
| MaeII | ACGT | 2 cut(s) | 30, 685 |
| MaeIII | GTNAC | 2 cut(s) | 982, 1045 |
| MbiI | CCGCTC | 1 cut(s) | 800 |
| MboII | GAAGA | 3 cut(s) | 557, 819, 822 |
| MflI | RGATCY | 2 cut(s) | 238, 462 |
| MhlI | GDGCHC | 1 cut(s) | 392 |
| MlsI | TGGCCA | 1 cut(s) | 744 |
| MluCI | AATT | 7 cut(s) | 23, 300, 577, 603, 616, 935, 949 |
| MluNI | TGGCCA | 1 cut(s) | 744 |
| MlyI | GAGTC | 3 cut(s) | 408, 643, 685 |
| MmeI | TCCRAC | 1 cut(s) | 346 |
| MnlI | CCTC | 8 cut(s) | 8, 44, 153, 476, 495, 718, 813, 816 |
| Mox20I | TGGCCA | 1 cut(s) | 744 |
| MroI | TCCGGA | 1 cut(s) | 607 |
| MroNI | GCCGGC | 1 cut(s) | 779 |
| MscI | TGGCCA | 1 cut(s) | 744 |
| MseI | TTAA | 2 cut(s) | 354, 524 |
| MslI | CAYNNNNRTG | 2 cut(s) | 669, 702 |
| Msp20I | TGGCCA | 1 cut(s) | 744 |
| MspA1I | CMGCKG | 1 cut(s) | 455 |
| MspI | CCGG | 4 cut(s) | 608, 771, 780, 1050 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 580, 741, 789 |
| NaeI | GCCGGC | 1 cut(s) | 781 |
| NcoI | CCATGG | 1 cut(s) | 43 |
| NgoMIV | GCCGGC | 1 cut(s) | 779 |
| NlaIII | CATG | 6 cut(s) | 47, 175, 396, 424, 472, 562 |
| NlaIV | GGNNCC | 7 cut(s) | 49, 138, 595, 775, 784, 796, 865 |
| NmuCI | GTSAC | 1 cut(s) | 1045 |
| NsbI | TGCGCA | 1 cut(s) | 206 |
| OliI | CACNNNNGTG | 1 cut(s) | 702 |
| PagI | TCATGA | 1 cut(s) | 468 |
| PciSI | GCTCTTC | 1 cut(s) | 832 |
| PdiI | GCCGGC | 1 cut(s) | 781 |
| PfeI | GAWTC | 1 cut(s) | 611 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 756 |
| PinAI | ACCGGT | 1 cut(s) | 770 |
| PkrI | GCNGC | 6 cut(s) | 457, 521, 778, 799, 816, 916 |
| PleI | GAGTC | 3 cut(s) | 407, 642, 685 |
| PmaCI | CACGTG | 1 cut(s) | 686 |
| PmlI | CACGTG | 1 cut(s) | 686 |
| PpsI | GAGTC | 3 cut(s) | 407, 642, 685 |
| Ppu21I | YACGTR | 1 cut(s) | 686 |
| Psp124BI | GAGCTC | 1 cut(s) | 392 |
| PspCI | CACGTG | 1 cut(s) | 686 |
| PspFI | CCCAGC | 2 cut(s) | 63, 309 |
| PspN4I | GGNNCC | 7 cut(s) | 49, 138, 595, 775, 784, 796, 865 |
| PspPI | GGNCC | 4 cut(s) | 105, 782, 864, 871 |
| PsuI | RGATCY | 2 cut(s) | 238, 462 |
| PvuII | CAGCTG | 1 cut(s) | 455 |
| RsaI | GTAC | 2 cut(s) | 49, 56 |
| RsaNI | GTAC | 2 cut(s) | 48, 55 |
| RseI | CAYNNNNRTG | 2 cut(s) | 669, 702 |
| Rsr2I | CGGWCCG | 1 cut(s) | 105 |
| RsrII | CGGWCCG | 1 cut(s) | 105 |
| SacI | GAGCTC | 1 cut(s) | 392 |
| SapI | GCTCTTC | 1 cut(s) | 832 |
| SaqAI | TTAA | 2 cut(s) | 354, 524 |
| SatI | GCNGC | 6 cut(s) | 456, 520, 777, 798, 815, 915 |
| Sau96I | GGNCC | 4 cut(s) | 105, 782, 864, 871 |
| SchI | GAGTC | 3 cut(s) | 408, 643, 685 |
| SduI | GDGCHC | 1 cut(s) | 392 |
| SfaNI | GCATC | 3 cut(s) | 238, 403, 1018 |
| SgrAI | CRCCGGYG | 1 cut(s) | 770 |
| SinI | GGWCC | 3 cut(s) | 105, 864, 871 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 669, 702 |
| SmlI | CTYRAG | 1 cut(s) | 485 |
| SmoI | CTYRAG | 1 cut(s) | 485 |
| Sse9I | AATT | 7 cut(s) | 23, 300, 577, 603, 616, 935, 949 |
| SsiI | CCGC | 7 cut(s) | 140, 519, 631, 777, 792, 798, 915 |
| SstI | GAGCTC | 1 cut(s) | 392 |
| StyI | CCWWGG | 3 cut(s) | 43, 786, 867 |
| TaaI | ACNGT | 2 cut(s) | 704, 845 |
| TaiI | ACGT | 2 cut(s) | 33, 688 |
| TaqI | TCGA | 7 cut(s) | 27, 192, 281, 402, 426, 440, 763 |
| TaqII | GACCGA | 1 cut(s) | 122 |
| TasI | AATT | 7 cut(s) | 23, 300, 577, 603, 616, 935, 949 |
| TauI | GCSGC | 4 cut(s) | 522, 779, 800, 917 |
| TfiI | GAWTC | 1 cut(s) | 611 |
| Tru1I | TTAA | 2 cut(s) | 354, 524 |
| Tru9I | TTAA | 2 cut(s) | 354, 524 |
| TscAI | CASTG | 3 cut(s) | 207, 881, 961 |
| TseFI | GTSAC | 1 cut(s) | 1045 |
| TseI | GCWGC | 2 cut(s) | 455, 814 |
| Tsp45I | GTSAC | 1 cut(s) | 1045 |
| TspDTI | ATGAA | 4 cut(s) | 79, 330, 485, 687 |
| TspRI | CASTG | 3 cut(s) | 207, 881, 961 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 756 |
| VpaK11BI | GGWCC | 3 cut(s) | 105, 864, 871 |
| XapI | RAATTY | 2 cut(s) | 23, 603 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 240, 707 |
| ZraI | GACGTC | 1 cut(s) | 31 |