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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 319 |
| AclWI | GGATC | 3 cut(s) | 2, 116, 185 |
| AcuI | CTGAAG | 1 cut(s) | 200 |
| AfaI | GTAC | 2 cut(s) | 56, 212 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 64, 205, 295 |
| AflIII | ACRYGT | 1 cut(s) | 360 |
| AgsI | TTSAA | 2 cut(s) | 223, 352 |
| AjnI | CCWGG | 1 cut(s) | 289 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 285, 317 |
| AlwI | GGATC | 3 cut(s) | 2, 116, 185 |
| AspLEI | GCGC | 1 cut(s) | 369 |
| AsuHPI | GGTGA | 1 cut(s) | 163 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 38, 71 |
| BbsI | GAAGAC | 1 cut(s) | 230 |
| BccI | CCATC | 1 cut(s) | 66 |
| BciT130I | CCWGG | 1 cut(s) | 291 |
| BfaI | CTAG | 1 cut(s) | 195 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 92 |
| Bme1390I | CCNGG | 1 cut(s) | 291 |
| BmrFI | CCNGG | 1 cut(s) | 291 |
| BmsI | GCATC | 2 cut(s) | 151, 235 |
| BpiI | GAAGAC | 1 cut(s) | 230 |
| BpuEI | CTTGAG | 1 cut(s) | 71 |
| BsaJI | CCNNGG | 1 cut(s) | 289 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 64, 205, 295 |
| Bse1I | ACTGG | 1 cut(s) | 300 |
| BseBI | CCWGG | 1 cut(s) | 291 |
| BseDI | CCNNGG | 1 cut(s) | 289 |
| BseGI | GGATG | 1 cut(s) | 250 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 64, 205, 295 |
| BseMII | CTCAG | 3 cut(s) | 12, 54, 167 |
| BseNI | ACTGG | 1 cut(s) | 300 |
| BseYI | CCCAGC | 1 cut(s) | 204 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 64, 205, 295 |
| Bsp143I | GATC | 3 cut(s) | 7, 121, 190 |
| BspACI | CCGC | 1 cut(s) | 319 |
| BspCNI | CTCAG | 3 cut(s) | 13, 53, 168 |
| BspPI | GGATC | 3 cut(s) | 2, 116, 185 |
| BsrI | ACTGG | 1 cut(s) | 300 |
| BssECI | CCNNGG | 1 cut(s) | 289 |
| BssMI | GATC | 3 cut(s) | 7, 121, 190 |
| Bst2UI | CCWGG | 1 cut(s) | 291 |
| Bst4CI | ACNGT | 1 cut(s) | 240 |
| BstC8I | GCNNGC | 1 cut(s) | 323 |
| BstDEI | CTNAG | 3 cut(s) | 21, 40, 176 |
| BstF5I | GGATG | 1 cut(s) | 250 |
| BstHHI | GCGC | 1 cut(s) | 369 |
| BstKTI | GATC | 3 cut(s) | 10, 124, 193 |
| BstMBI | GATC | 3 cut(s) | 7, 121, 190 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 92 |
| BstNI | CCWGG | 1 cut(s) | 291 |
| BstNSI | RCATGY | 1 cut(s) | 364 |
| BstSCI | CCNGG | 1 cut(s) | 289 |
| BstV2I | GAAGAC | 1 cut(s) | 230 |
| BtsCI | GGATG | 1 cut(s) | 250 |
| BtsI | GCAGTG | 1 cut(s) | 105 |
| BtsIMutI | CAGTG | 3 cut(s) | 105, 236, 311 |
| Cac8I | GCNNGC | 1 cut(s) | 323 |
| CfoI | GCGC | 1 cut(s) | 369 |
| Csp6I | GTAC | 2 cut(s) | 55, 211 |
| CviAII | CATG | 1 cut(s) | 361 |
| CviJI | RGCY | 3 cut(s) | 5, 198, 299 |
| CviKI_1 | RGCY | 3 cut(s) | 5, 198, 299 |
| CviQI | GTAC | 2 cut(s) | 55, 211 |
| DdeI | CTNAG | 3 cut(s) | 21, 40, 176 |
| DpnI | GATC | 3 cut(s) | 9, 123, 192 |
| DpnII | GATC | 3 cut(s) | 7, 121, 190 |
| Eco57I | CTGAAG | 1 cut(s) | 200 |
| EcoRII | CCWGG | 1 cut(s) | 289 |
| FaeI | CATG | 1 cut(s) | 364 |
| FaiI | YATR | 6 cut(s) | 77, 186, 333, 362, 391, 419 |
| FatI | CATG | 1 cut(s) | 360 |
| FokI | GGATG | 1 cut(s) | 257 |
| FspBI | CTAG | 1 cut(s) | 195 |
| GlaI | GCGC | 1 cut(s) | 368 |
| GsaI | CCCAGC | 1 cut(s) | 208 |
| HhaI | GCGC | 1 cut(s) | 369 |
| Hin1II | CATG | 1 cut(s) | 364 |
| Hin6I | GCGC | 1 cut(s) | 367 |
| HinP1I | GCGC | 1 cut(s) | 367 |
| HinfI | GANTC | 3 cut(s) | 38, 315, 430 |
| HphI | GGTGA | 1 cut(s) | 163 |
| Hpy188I | TCNGA | 3 cut(s) | 43, 73, 177 |
| Hpy188III | TCNNGA | 1 cut(s) | 275 |
| HpyAV | CCTTC | 3 cut(s) | 38, 224, 390 |
| HpyCH4III | ACNGT | 1 cut(s) | 240 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 92 |
| HpyF3I | CTNAG | 3 cut(s) | 21, 40, 176 |
| Hsp92II | CATG | 1 cut(s) | 364 |
| HspAI | GCGC | 1 cut(s) | 367 |
| Kzo9I | GATC | 3 cut(s) | 7, 121, 190 |
| LmnI | GCTCC | 1 cut(s) | 291 |
| LpnPI | CCDG | 5 cut(s) | 33, 190, 276, 281, 303 |
| LweI | GCATC | 2 cut(s) | 151, 235 |
| MaeI | CTAG | 1 cut(s) | 195 |
| MalI | GATC | 3 cut(s) | 9, 123, 192 |
| MboI | GATC | 3 cut(s) | 7, 121, 190 |
| MboII | GAAGA | 2 cut(s) | 235, 422 |
| MluCI | AATT | 2 cut(s) | 168, 385 |
| MlyI | GAGTC | 2 cut(s) | 32, 424 |
| MmeI | TCCRAC | 1 cut(s) | 35 |
| MnlI | CCTC | 1 cut(s) | 171 |
| MseI | TTAA | 5 cut(s) | 167, 252, 371, 384, 451 |
| MspR9I | CCNGG | 1 cut(s) | 291 |
| MvaI | CCWGG | 1 cut(s) | 291 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 92 |
| NdeII | GATC | 3 cut(s) | 7, 121, 190 |
| NlaIII | CATG | 1 cut(s) | 364 |
| NspI | RCATGY | 1 cut(s) | 364 |
| PciI | ACATGT | 1 cut(s) | 360 |
| PfeI | GAWTC | 1 cut(s) | 315 |
| PleI | GAGTC | 2 cut(s) | 32, 424 |
| PpsI | GAGTC | 2 cut(s) | 32, 424 |
| PscI | ACATGT | 1 cut(s) | 360 |
| Psp6I | CCWGG | 1 cut(s) | 289 |
| PspFI | CCCAGC | 1 cut(s) | 204 |
| PspGI | CCWGG | 1 cut(s) | 289 |
| RsaI | GTAC | 2 cut(s) | 56, 212 |
| RsaNI | GTAC | 2 cut(s) | 55, 211 |
| SaqAI | TTAA | 5 cut(s) | 167, 252, 371, 384, 451 |
| Sau3AI | GATC | 3 cut(s) | 7, 121, 190 |
| SchI | GAGTC | 2 cut(s) | 32, 424 |
| ScrFI | CCNGG | 1 cut(s) | 291 |
| SetI | ASST | 4 cut(s) | 22, 153, 216, 342 |
| SfaNI | GCATC | 2 cut(s) | 151, 235 |
| SmlI | CTYRAG | 1 cut(s) | 50 |
| SmoI | CTYRAG | 1 cut(s) | 50 |
| Sse9I | AATT | 2 cut(s) | 168, 385 |
| SsiI | CCGC | 1 cut(s) | 319 |
| SspMI | CTAG | 1 cut(s) | 195 |
| StyD4I | CCNGG | 1 cut(s) | 289 |
| TaaI | ACNGT | 1 cut(s) | 240 |
| TaqI | TCGA | 1 cut(s) | 16 |
| TasI | AATT | 2 cut(s) | 168, 385 |
| TfiI | GAWTC | 1 cut(s) | 315 |
| Tru1I | TTAA | 5 cut(s) | 167, 252, 371, 384, 451 |
| Tru9I | TTAA | 5 cut(s) | 167, 252, 371, 384, 451 |
| TscAI | CASTG | 3 cut(s) | 112, 243, 311 |
| TspDTI | ATGAA | 1 cut(s) | 150 |
| TspRI | CASTG | 3 cut(s) | 112, 243, 311 |
| XceI | RCATGY | 1 cut(s) | 364 |
| XspI | CTAG | 1 cut(s) | 195 |