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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 2 cut(s) | 192, 657 |
| AclWI | GGATC | 3 cut(s) | 2, 301, 551 |
| AcsI | RAATTY | 3 cut(s) | 23, 271, 486 |
| AcuI | CTGAAG | 3 cut(s) | 86, 321, 713 |
| AcvI | CACGTG | 1 cut(s) | 596 |
| AdeI | CACNNNGTG | 1 cut(s) | 596 |
| AfaI | GTAC | 3 cut(s) | 56, 98, 793 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 64, 192, 566, 657 |
| AflII | CTTAAG | 1 cut(s) | 137 |
| AgsI | TTSAA | 4 cut(s) | 109, 238, 400, 454 |
| AjiI | CACGTC | 2 cut(s) | 266, 551 |
| AjnI | CCWGG | 1 cut(s) | 464 |
| AluBI | AGCT | 1 cut(s) | 406 |
| AluI | AGCT | 1 cut(s) | 406 |
| AlwI | GGATC | 3 cut(s) | 2, 301, 551 |
| AoxI | GGCC | 3 cut(s) | 467, 634, 643 |
| ApoI | RAATTY | 3 cut(s) | 23, 271, 486 |
| AspS9I | GGNCC | 1 cut(s) | 634 |
| AsuNHI | GCTAGC | 1 cut(s) | 677 |
| BaeGI | GKGCMC | 1 cut(s) | 717 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 38, 71 |
| BbrPI | CACGTG | 1 cut(s) | 596 |
| BbsI | GAAGAC | 2 cut(s) | 116, 320 |
| BccI | CCATC | 2 cut(s) | 654, 662 |
| BciT130I | CCWGG | 1 cut(s) | 466 |
| BclI | TGATCA | 1 cut(s) | 408 |
| BfaI | CTAG | 3 cut(s) | 350, 678, 684 |
| BfmI | CTRYAG | 1 cut(s) | 765 |
| BfrI | CTTAAG | 1 cut(s) | 137 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 642 |
| Bme1390I | CCNGG | 1 cut(s) | 466 |
| BmgBI | CACGTC | 2 cut(s) | 266, 551 |
| BmgT120I | GGNCC | 1 cut(s) | 634 |
| BmrFI | CCNGG | 1 cut(s) | 466 |
| BmsI | GCATC | 1 cut(s) | 121 |
| BmtI | GCTAGC | 1 cut(s) | 681 |
| BpiI | GAAGAC | 2 cut(s) | 116, 320 |
| BpuEI | CTTGAG | 1 cut(s) | 71 |
| BsaAI | YACGTR | 1 cut(s) | 596 |
| BsaJI | CCNNGG | 2 cut(s) | 492, 637 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 64, 192, 566, 657 |
| Bse1I | ACTGG | 1 cut(s) | 358 |
| Bse3DI | GCAATG | 1 cut(s) | 759 |
| BseBI | CCWGG | 1 cut(s) | 466 |
| BseDI | CCNNGG | 2 cut(s) | 492, 637 |
| BseGI | GGATG | 2 cut(s) | 136, 646 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 64, 192, 566, 657 |
| BseMI | GCAATG | 1 cut(s) | 759 |
| BseMII | CTCAG | 3 cut(s) | 54, 420, 579 |
| BseNI | ACTGG | 1 cut(s) | 358 |
| BseSI | GKGCMC | 1 cut(s) | 717 |
| BshFI | GGCC | 3 cut(s) | 469, 636, 645 |
| BslFI | GGGAC | 3 cut(s) | 156, 490, 627 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 64, 192, 566, 657 |
| BsmFI | GGGAC | 3 cut(s) | 156, 490, 627 |
| BsnI | GGCC | 3 cut(s) | 469, 636, 645 |
| Bsp1286I | GDGCHC | 1 cut(s) | 717 |
| Bsp143I | GATC | 4 cut(s) | 7, 306, 408, 556 |
| BspANI | GGCC | 3 cut(s) | 469, 636, 645 |
| BspCNI | CTCAG | 3 cut(s) | 53, 421, 578 |
| BspHI | TCATGA | 1 cut(s) | 160 |
| BspOI | GCTAGC | 1 cut(s) | 681 |
| BspPI | GGATC | 3 cut(s) | 2, 301, 551 |
| BspTI | CTTAAG | 1 cut(s) | 137 |
| BsrDI | GCAATG | 1 cut(s) | 759 |
| BsrI | ACTGG | 1 cut(s) | 358 |
| BssECI | CCNNGG | 2 cut(s) | 492, 637 |
| BssMI | GATC | 4 cut(s) | 7, 306, 408, 556 |
| BssT1I | CCWWGG | 1 cut(s) | 637 |
| Bst2UI | CCWGG | 1 cut(s) | 466 |
| Bst4CI | ACNGT | 2 cut(s) | 62, 85 |
| BstAFI | CTTAAG | 1 cut(s) | 137 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 587, 596 |
| BstBAI | YACGTR | 1 cut(s) | 596 |
| BstC8I | GCNNGC | 3 cut(s) | 209, 679, 810 |
| BstDEI | CTNAG | 3 cut(s) | 40, 429, 565 |
| BstDSI | CCRYGG | 1 cut(s) | 492 |
| BstF5I | GGATG | 2 cut(s) | 136, 646 |
| BstKTI | GATC | 4 cut(s) | 10, 309, 411, 559 |
| BstMBI | GATC | 4 cut(s) | 7, 306, 408, 556 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 587, 596, 642, 712, 809 |
| BstNI | CCWGG | 1 cut(s) | 466 |
| BstNSI | RCATGY | 1 cut(s) | 590 |
| BstSCI | CCNGG | 1 cut(s) | 464 |
| BstSFI | CTRYAG | 1 cut(s) | 765 |
| BstSLI | GKGCMC | 1 cut(s) | 717 |
| BstV2I | GAAGAC | 2 cut(s) | 116, 320 |
| BsuRI | GGCC | 3 cut(s) | 469, 636, 645 |
| BtgI | CCRYGG | 1 cut(s) | 492 |
| BtrI | CACGTC | 2 cut(s) | 266, 551 |
| BtsCI | GGATG | 2 cut(s) | 136, 646 |
| BtsIMutI | CAGTG | 1 cut(s) | 197 |
| Cac8I | GCNNGC | 3 cut(s) | 209, 679, 810 |
| CciI | TCATGA | 1 cut(s) | 160 |
| Cfr13I | GGNCC | 1 cut(s) | 634 |
| CseI | GACGC | 1 cut(s) | 242 |
| Csp6I | GTAC | 3 cut(s) | 55, 97, 792 |
| CviAII | CATG | 6 cut(s) | 161, 587, 601, 617, 692, 761 |
| CviJI | RGCY | 8 cut(s) | 367, 406, 428, 469, 497, 636, 645, 681 |
| CviKI_1 | RGCY | 8 cut(s) | 367, 406, 428, 469, 497, 636, 645, 681 |
| CviQI | GTAC | 3 cut(s) | 55, 97, 792 |
| DdeI | CTNAG | 3 cut(s) | 40, 429, 565 |
| DpnI | GATC | 4 cut(s) | 9, 308, 410, 558 |
| DpnII | GATC | 4 cut(s) | 7, 306, 408, 556 |
| DraIII | CACNNNGTG | 1 cut(s) | 596 |
| Eco130I | CCWWGG | 1 cut(s) | 637 |
| Eco147I | AGGCCT | 1 cut(s) | 469 |
| Eco57I | CTGAAG | 3 cut(s) | 86, 321, 713 |
| Eco72I | CACGTG | 1 cut(s) | 596 |
| EcoRII | CCWGG | 1 cut(s) | 464 |
| EcoT14I | CCWWGG | 1 cut(s) | 637 |
| ErhI | CCWWGG | 1 cut(s) | 637 |
| FaeI | CATG | 6 cut(s) | 164, 590, 604, 620, 695, 764 |
| FaqI | GGGAC | 3 cut(s) | 156, 490, 627 |
| FatI | CATG | 6 cut(s) | 160, 586, 600, 616, 691, 760 |
| FbaI | TGATCA | 1 cut(s) | 408 |
| FokI | GGATG | 2 cut(s) | 143, 633 |
| FspBI | CTAG | 3 cut(s) | 350, 678, 684 |
| HaeIII | GGCC | 3 cut(s) | 469, 636, 645 |
| HgaI | GACGC | 1 cut(s) | 242 |
| Hin1II | CATG | 6 cut(s) | 164, 590, 604, 620, 695, 764 |
| HinfI | GANTC | 4 cut(s) | 38, 316, 387, 776 |
| Hpy188I | TCNGA | 2 cut(s) | 43, 73 |
| Hpy188III | TCNNGA | 2 cut(s) | 161, 310 |
| HpyAV | CCTTC | 4 cut(s) | 38, 110, 406, 737 |
| HpyCH4III | ACNGT | 2 cut(s) | 62, 85 |
| HpyCH4IV | ACGT | 4 cut(s) | 145, 265, 550, 595 |
| HpyCH4V | TGCA | 6 cut(s) | 421, 536, 581, 590, 609, 706 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 587, 596, 642, 712, 809 |
| HpyF3I | CTNAG | 3 cut(s) | 40, 429, 565 |
| HpySE526I | ACGT | 4 cut(s) | 145, 265, 550, 595 |
| Hsp92II | CATG | 6 cut(s) | 164, 590, 604, 620, 695, 764 |
| Ksp22I | TGATCA | 1 cut(s) | 408 |
| Kzo9I | GATC | 4 cut(s) | 7, 306, 408, 556 |
| LmnI | GCTCC | 1 cut(s) | 177 |
| LpnPI | CCDG | 9 cut(s) | 33, 189, 323, 371, 451, 478, 483, 552, 724 |
| LweI | GCATC | 1 cut(s) | 121 |
| MaeI | CTAG | 3 cut(s) | 350, 678, 684 |
| MaeII | ACGT | 4 cut(s) | 145, 265, 550, 595 |
| MalI | GATC | 4 cut(s) | 9, 308, 410, 558 |
| MboI | GATC | 4 cut(s) | 7, 306, 408, 556 |
| MboII | GAAGA | 3 cut(s) | 121, 308, 325 |
| MhlI | GDGCHC | 1 cut(s) | 717 |
| MluCI | AATT | 6 cut(s) | 23, 122, 271, 342, 486, 499 |
| MlyI | GAGTC | 3 cut(s) | 32, 310, 785 |
| MmeI | TCCRAC | 2 cut(s) | 35, 526 |
| MnlI | CCTC | 2 cut(s) | 426, 635 |
| MseI | TTAA | 1 cut(s) | 138 |
| MslI | CAYNNNNRTG | 2 cut(s) | 651, 666 |
| MspCI | CTTAAG | 1 cut(s) | 137 |
| MspR9I | CCNGG | 1 cut(s) | 466 |
| MvaI | CCWGG | 1 cut(s) | 466 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 587, 596, 642, 712, 809 |
| NdeII | GATC | 4 cut(s) | 7, 306, 408, 556 |
| NheI | GCTAGC | 1 cut(s) | 677 |
| NlaIII | CATG | 6 cut(s) | 164, 590, 604, 620, 695, 764 |
| NspI | RCATGY | 1 cut(s) | 590 |
| PagI | TCATGA | 1 cut(s) | 160 |
| PceI | AGGCCT | 1 cut(s) | 469 |
| PfeI | GAWTC | 1 cut(s) | 387 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 192, 657 |
| PleI | GAGTC | 3 cut(s) | 32, 310, 784 |
| PmaCI | CACGTG | 1 cut(s) | 596 |
| PmlI | CACGTG | 1 cut(s) | 596 |
| PpsI | GAGTC | 3 cut(s) | 32, 310, 784 |
| Ppu21I | YACGTR | 1 cut(s) | 596 |
| Psp6I | CCWGG | 1 cut(s) | 464 |
| PspCI | CACGTG | 1 cut(s) | 596 |
| PspGI | CCWGG | 1 cut(s) | 464 |
| PspPI | GGNCC | 1 cut(s) | 634 |
| RsaI | GTAC | 3 cut(s) | 56, 98, 793 |
| RsaNI | GTAC | 3 cut(s) | 55, 97, 792 |
| RseI | CAYNNNNRTG | 2 cut(s) | 651, 666 |
| SaqAI | TTAA | 1 cut(s) | 138 |
| Sau3AI | GATC | 4 cut(s) | 7, 306, 408, 556 |
| Sau96I | GGNCC | 1 cut(s) | 634 |
| SchI | GAGTC | 3 cut(s) | 32, 310, 785 |
| ScrFI | CCNGG | 1 cut(s) | 466 |
| SduI | GDGCHC | 1 cut(s) | 717 |
| SfaNI | GCATC | 1 cut(s) | 121 |
| SfcI | CTRYAG | 1 cut(s) | 765 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 642 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 651, 666 |
| SmlI | CTYRAG | 2 cut(s) | 50, 137 |
| SmoI | CTYRAG | 2 cut(s) | 50, 137 |
| Sse9I | AATT | 6 cut(s) | 23, 122, 271, 342, 486, 499 |
| SseBI | AGGCCT | 1 cut(s) | 469 |
| SspMI | CTAG | 3 cut(s) | 350, 678, 684 |
| StuI | AGGCCT | 1 cut(s) | 469 |
| StyD4I | CCNGG | 1 cut(s) | 464 |
| StyI | CCWWGG | 1 cut(s) | 637 |
| TaaI | ACNGT | 2 cut(s) | 62, 85 |
| TaiI | ACGT | 4 cut(s) | 148, 268, 553, 598 |
| TaqI | TCGA | 1 cut(s) | 16 |
| TasI | AATT | 6 cut(s) | 23, 122, 271, 342, 486, 499 |
| TfiI | GAWTC | 1 cut(s) | 387 |
| Tru1I | TTAA | 1 cut(s) | 138 |
| Tru9I | TTAA | 1 cut(s) | 138 |
| TscAI | CASTG | 1 cut(s) | 197 |
| TspDTI | ATGAA | 4 cut(s) | 149, 213, 456, 770 |
| TspGWI | ACGGA | 1 cut(s) | 481 |
| TspRI | CASTG | 1 cut(s) | 197 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 192, 657 |
| Vha464I | CTTAAG | 1 cut(s) | 137 |
| XapI | RAATTY | 3 cut(s) | 23, 271, 486 |
| XceI | RCATGY | 1 cut(s) | 590 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 600 |
| XspI | CTAG | 3 cut(s) | 350, 678, 684 |