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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 177 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 49, 251 |
| AccII | CGCG | 2 cut(s) | 276, 330 |
| AccIII | TCCGGA | 1 cut(s) | 156 |
| AciI | CCGC | 5 cut(s) | 13, 63, 248, 372, 487 |
| AclWI | GGATC | 3 cut(s) | 167, 593, 707 |
| AcuI | CTGAAG | 1 cut(s) | 833 |
| AfaI | GTAC | 1 cut(s) | 647 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 49, 251, 352, 371, 483, 518, 655, 796 |
| AflII | CTTAAG | 1 cut(s) | 842 |
| AflIII | ACRYGT | 1 cut(s) | 535 |
| AgsI | TTSAA | 1 cut(s) | 225 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 539 |
| AjiI | CACGTC | 1 cut(s) | 536 |
| AjnI | CCWGG | 3 cut(s) | 165, 250, 514 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 484, 516 |
| AluBI | AGCT | 3 cut(s) | 578, 596, 811 |
| AluI | AGCT | 3 cut(s) | 578, 596, 811 |
| Alw21I | GWGCWC | 1 cut(s) | 145 |
| Alw26I | GTCTC | 1 cut(s) | 321 |
| AlwI | GGATC | 3 cut(s) | 167, 593, 707 |
| Aor13HI | TCCGGA | 1 cut(s) | 156 |
| AoxI | GGCC | 5 cut(s) | 96, 258, 380, 399, 418 |
| ApeKI | GCWGC | 2 cut(s) | 10, 562 |
| AspS9I | GGNCC | 5 cut(s) | 120, 242, 380, 399, 418 |
| AsuHPI | GGTGA | 1 cut(s) | 754 |
| AvaII | GGWCC | 2 cut(s) | 120, 242 |
| BaeGI | GKGCMC | 1 cut(s) | 180 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 629, 662 |
| BanI | GGYRCC | 1 cut(s) | 177 |
| BbsI | GAAGAC | 1 cut(s) | 821 |
| Bbv12I | GWGCWC | 1 cut(s) | 145 |
| BbvCI | CCTCAGC | 1 cut(s) | 5 |
| BbvI | GCAGC | 1 cut(s) | 574 |
| BccI | CCATC | 3 cut(s) | 199, 263, 657 |
| BceAI | ACGGC | 2 cut(s) | 349, 426 |
| BciT130I | CCWGG | 3 cut(s) | 167, 252, 516 |
| BcoDI | GTCTC | 1 cut(s) | 321 |
| BfaI | CTAG | 3 cut(s) | 441, 479, 786 |
| BfmI | CTRYAG | 1 cut(s) | 806 |
| BfrI | CTTAAG | 1 cut(s) | 842 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 683 |
| BglII | AGATCT | 1 cut(s) | 204 |
| BisI | GCNGC | 3 cut(s) | 11, 63, 563 |
| BlsI | GCNGC | 3 cut(s) | 12, 64, 564 |
| Bme1390I | CCNGG | 3 cut(s) | 167, 252, 516 |
| Bme18I | GGWCC | 2 cut(s) | 120, 242 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 539 |
| BmgBI | CACGTC | 1 cut(s) | 536 |
| BmgT120I | GGNCC | 5 cut(s) | 120, 242, 380, 399, 418 |
| BmiI | GGNNCC | 4 cut(s) | 121, 179, 243, 363 |
| BmrFI | CCNGG | 3 cut(s) | 167, 252, 516 |
| BmrI | ACTGGG | 1 cut(s) | 183 |
| BmsI | GCATC | 1 cut(s) | 62 |
| BmuI | ACTGGG | 1 cut(s) | 183 |
| BpiI | GAAGAC | 1 cut(s) | 821 |
| Bpu10I | CCTNAGC | 1 cut(s) | 5 |
| BpuEI | CTTGAG | 1 cut(s) | 662 |
| BsaJI | CCNNGG | 3 cut(s) | 33, 66, 181 |
| BsaWI | WCCGGW | 1 cut(s) | 156 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 49, 251, 352, 371, 483, 518, 655, 796 |
| Bse1I | ACTGG | 1 cut(s) | 178 |
| BseAI | TCCGGA | 1 cut(s) | 156 |
| BseBI | CCWGG | 3 cut(s) | 167, 252, 516 |
| BseDI | CCNNGG | 3 cut(s) | 33, 66, 181 |
| BseGI | GGATG | 2 cut(s) | 531, 854 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 49, 251, 352, 371, 483, 518, 655, 796 |
| BseMII | CTCAG | 3 cut(s) | 332, 603, 645 |
| BseNI | ACTGG | 1 cut(s) | 178 |
| BseSI | GKGCMC | 1 cut(s) | 180 |
| BseXI | GCAGC | 1 cut(s) | 574 |
| BseYI | CCCAGC | 1 cut(s) | 795 |
| Bsh1236I | CGCG | 2 cut(s) | 276, 330 |
| BshFI | GGCC | 5 cut(s) | 98, 260, 382, 401, 420 |
| BshNI | GGYRCC | 1 cut(s) | 177 |
| BsiHKAI | GWGCWC | 1 cut(s) | 145 |
| BsiSI | CCGG | 2 cut(s) | 99, 157 |
| BslFI | GGGAC | 3 cut(s) | 133, 255, 339 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 49, 251, 352, 371, 483, 518, 655, 796 |
| BsmAI | GTCTC | 1 cut(s) | 321 |
| BsmBI | CGTCTC | 1 cut(s) | 321 |
| BsmFI | GGGAC | 3 cut(s) | 133, 255, 339 |
| BsmI | GAATGC | 1 cut(s) | 841 |
| BsnI | GGCC | 5 cut(s) | 98, 260, 382, 401, 420 |
| Bsp1286I | GDGCHC | 2 cut(s) | 145, 180 |
| Bsp13I | TCCGGA | 1 cut(s) | 156 |
| Bsp143I | GATC | 6 cut(s) | 159, 204, 556, 598, 712, 721 |
| Bsp19I | CCATGG | 2 cut(s) | 33, 181 |
| Bsp68I | TCGCGA | 1 cut(s) | 276 |
| BspACI | CCGC | 5 cut(s) | 13, 63, 248, 372, 487 |
| BspANI | GGCC | 5 cut(s) | 98, 260, 382, 401, 420 |
| BspCNI | CTCAG | 3 cut(s) | 331, 604, 644 |
| BspEI | TCCGGA | 1 cut(s) | 156 |
| BspFNI | CGCG | 2 cut(s) | 276, 330 |
| BspHI | TCATGA | 1 cut(s) | 724 |
| BspLI | GGNNCC | 4 cut(s) | 121, 179, 243, 363 |
| BspPI | GGATC | 3 cut(s) | 167, 593, 707 |
| BspT107I | GGYRCC | 1 cut(s) | 177 |
| BspTI | CTTAAG | 1 cut(s) | 842 |
| BsrI | ACTGG | 1 cut(s) | 178 |
| BssECI | CCNNGG | 3 cut(s) | 33, 66, 181 |
| BssMI | GATC | 6 cut(s) | 159, 204, 556, 598, 712, 721 |
| BssT1I | CCWWGG | 2 cut(s) | 33, 181 |
| Bst2UI | CCWGG | 3 cut(s) | 167, 252, 516 |
| Bst4CI | ACNGT | 2 cut(s) | 308, 892 |
| BstAFI | CTTAAG | 1 cut(s) | 842 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 59, 149, 505 |
| BstC8I | GCNNGC | 1 cut(s) | 497 |
| BstDEI | CTNAG | 4 cut(s) | 5, 318, 612, 631 |
| BstDSI | CCRYGG | 2 cut(s) | 33, 181 |
| BstF5I | GGATG | 2 cut(s) | 531, 854 |
| BstFNI | CGCG | 2 cut(s) | 276, 330 |
| BstKTI | GATC | 6 cut(s) | 162, 207, 559, 601, 715, 724 |
| BstMAI | GTCTC | 1 cut(s) | 321 |
| BstMBI | GATC | 6 cut(s) | 159, 204, 556, 598, 712, 721 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 10, 16, 59, 149, 473, 505, 683 |
| BstNI | CCWGG | 3 cut(s) | 167, 252, 516 |
| BstNSI | RCATGY | 1 cut(s) | 499 |
| BstSCI | CCNGG | 3 cut(s) | 165, 250, 514 |
| BstSFI | CTRYAG | 1 cut(s) | 806 |
| BstSLI | GKGCMC | 1 cut(s) | 180 |
| BstUI | CGCG | 2 cut(s) | 276, 330 |
| BstV1I | GCAGC | 1 cut(s) | 574 |
| BstV2I | GAAGAC | 1 cut(s) | 821 |
| BstX2I | RGATCY | 2 cut(s) | 159, 204 |
| BstYI | RGATCY | 2 cut(s) | 159, 204 |
| BsuRI | GGCC | 5 cut(s) | 98, 260, 382, 401, 420 |
| BtgI | CCRYGG | 2 cut(s) | 33, 181 |
| BtrI | CACGTC | 1 cut(s) | 536 |
| BtsCI | GGATG | 2 cut(s) | 531, 854 |
| BtsI | GCAGTG | 1 cut(s) | 696 |
| BtsIMutI | CAGTG | 2 cut(s) | 261, 696 |
| BtuMI | TCGCGA | 1 cut(s) | 276 |
| Cac8I | GCNNGC | 1 cut(s) | 497 |
| CciI | TCATGA | 1 cut(s) | 724 |
| Cfr13I | GGNCC | 5 cut(s) | 120, 242, 380, 399, 418 |
| CseI | GACGC | 1 cut(s) | 336 |
| Csp6I | GTAC | 1 cut(s) | 646 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 289, 324 |
| CviAII | CATG | 4 cut(s) | 34, 182, 496, 725 |
| CviQI | GTAC | 1 cut(s) | 646 |
| DdeI | CTNAG | 4 cut(s) | 5, 318, 612, 631 |
| DpnI | GATC | 6 cut(s) | 161, 206, 558, 600, 714, 723 |
| DpnII | GATC | 6 cut(s) | 159, 204, 556, 598, 712, 721 |
| DriI | GACNNNNNGTC | 1 cut(s) | 539 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 539 |
| Eco130I | CCWWGG | 2 cut(s) | 33, 181 |
| Eco47I | GGWCC | 2 cut(s) | 120, 242 |
| Eco57I | CTGAAG | 1 cut(s) | 833 |
| EcoRII | CCWGG | 3 cut(s) | 165, 250, 514 |
| EcoT14I | CCWWGG | 2 cut(s) | 33, 181 |
| EcoT22I | ATGCAT | 1 cut(s) | 501 |
| ErhI | CCWWGG | 2 cut(s) | 33, 181 |
| Esp3I | CGTCTC | 1 cut(s) | 321 |
| FaeI | CATG | 4 cut(s) | 37, 185, 499, 728 |
| FaqI | GGGAC | 3 cut(s) | 133, 255, 339 |
| FatI | CATG | 4 cut(s) | 33, 181, 495, 724 |
| FauI | CCCGC | 3 cut(s) | 6, 365, 480 |
| Fnu4HI | GCNGC | 3 cut(s) | 11, 63, 563 |
| FokI | GGATG | 2 cut(s) | 538, 861 |
| Fsp4HI | GCNGC | 3 cut(s) | 11, 63, 563 |
| FspBI | CTAG | 3 cut(s) | 441, 479, 786 |
| GluI | GCNGC | 3 cut(s) | 11, 63, 563 |
| GsaI | CCCAGC | 1 cut(s) | 799 |
| HaeIII | GGCC | 5 cut(s) | 98, 260, 382, 401, 420 |
| HapII | CCGG | 2 cut(s) | 99, 157 |
| HgaI | GACGC | 1 cut(s) | 336 |
| Hin1II | CATG | 4 cut(s) | 37, 185, 499, 728 |
| HincII | GTYRAC | 2 cut(s) | 30, 340 |
| HindII | GTYRAC | 2 cut(s) | 30, 340 |
| HinfI | GANTC | 3 cut(s) | 102, 348, 629 |
| HpaI | GTTAAC | 1 cut(s) | 340 |
| HpaII | CCGG | 2 cut(s) | 99, 157 |
| HphI | GGTGA | 1 cut(s) | 754 |
| Hpy166II | GTNNAC | 3 cut(s) | 30, 237, 340 |
| Hpy188I | TCNGA | 6 cut(s) | 24, 107, 321, 408, 634, 664 |
| Hpy188III | TCNNGA | 4 cut(s) | 157, 275, 554, 725 |
| Hpy8I | GTNNAC | 3 cut(s) | 30, 237, 340 |
| Hpy99I | CGWCG | 3 cut(s) | 118, 308, 537 |
| HpyAV | CCTTC | 2 cut(s) | 326, 629 |
| HpyCH4III | ACNGT | 2 cut(s) | 308, 892 |
| HpyCH4IV | ACGT | 1 cut(s) | 535 |
| HpyCH4V | TGCA | 2 cut(s) | 499, 508 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 10, 16, 59, 149, 473, 505, 683 |
| HpyF3I | CTNAG | 4 cut(s) | 5, 318, 612, 631 |
| HpySE526I | ACGT | 1 cut(s) | 535 |
| Hsp92II | CATG | 4 cut(s) | 37, 185, 499, 728 |
| Kpn2I | TCCGGA | 1 cut(s) | 156 |
| KspAI | GTTAAC | 1 cut(s) | 340 |
| Kzo9I | GATC | 6 cut(s) | 159, 204, 556, 598, 712, 721 |
| LmnI | GCTCC | 2 cut(s) | 140, 361 |
| Lsp1109I | GCAGC | 1 cut(s) | 574 |
| LweI | GCATC | 1 cut(s) | 62 |
| MaeI | CTAG | 3 cut(s) | 441, 479, 786 |
| MaeII | ACGT | 1 cut(s) | 535 |
| MalI | GATC | 6 cut(s) | 161, 206, 558, 600, 714, 723 |
| MboI | GATC | 6 cut(s) | 159, 204, 556, 598, 712, 721 |
| MboII | GAAGA | 2 cut(s) | 138, 826 |
| MflI | RGATCY | 2 cut(s) | 159, 204 |
| MhlI | GDGCHC | 2 cut(s) | 145, 180 |
| MlyI | GAGTC | 2 cut(s) | 357, 623 |
| MmeI | TCCRAC | 2 cut(s) | 118, 626 |
| MnlI | CCTC | 6 cut(s) | 76, 315, 393, 477, 522, 762 |
| Mph1103I | ATGCAT | 1 cut(s) | 501 |
| MroI | TCCGGA | 1 cut(s) | 156 |
| MseI | TTAA | 3 cut(s) | 339, 758, 843 |
| MspA1I | CMGCKG | 1 cut(s) | 811 |
| MspCI | CTTAAG | 1 cut(s) | 842 |
| MspI | CCGG | 2 cut(s) | 99, 157 |
| MspR9I | CCNGG | 3 cut(s) | 167, 252, 516 |
| Mva1269I | GAATGC | 1 cut(s) | 841 |
| MvaI | CCWGG | 3 cut(s) | 167, 252, 516 |
| MvnI | CGCG | 2 cut(s) | 276, 330 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 10, 16, 59, 149, 473, 505, 683 |
| NcoI | CCATGG | 2 cut(s) | 33, 181 |
| NdeII | GATC | 6 cut(s) | 159, 204, 556, 598, 712, 721 |
| NlaIII | CATG | 4 cut(s) | 37, 185, 499, 728 |
| NlaIV | GGNNCC | 4 cut(s) | 121, 179, 243, 363 |
| NmeAIII | GCCGAG | 1 cut(s) | 371 |
| NruI | TCGCGA | 1 cut(s) | 276 |
| NsiI | ATGCAT | 1 cut(s) | 501 |
| NspI | RCATGY | 1 cut(s) | 499 |
| PaeI | GCATGC | 1 cut(s) | 499 |
| PagI | TCATGA | 1 cut(s) | 724 |
| PctI | GAATGC | 1 cut(s) | 841 |
| PfeI | GAWTC | 1 cut(s) | 102 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 49, 251 |
| PkrI | GCNGC | 3 cut(s) | 12, 64, 564 |
| PleI | GAGTC | 2 cut(s) | 356, 623 |
| PpsI | GAGTC | 2 cut(s) | 356, 623 |
| Psp6I | CCWGG | 3 cut(s) | 165, 250, 514 |
| PspFI | CCCAGC | 1 cut(s) | 795 |
| PspGI | CCWGG | 3 cut(s) | 165, 250, 514 |
| PspN4I | GGNNCC | 4 cut(s) | 121, 179, 243, 363 |
| PspPI | GGNCC | 5 cut(s) | 120, 242, 380, 399, 418 |
| PsuI | RGATCY | 2 cut(s) | 159, 204 |
| PvuII | CAGCTG | 1 cut(s) | 811 |
| RruI | TCGCGA | 1 cut(s) | 276 |
| RsaI | GTAC | 1 cut(s) | 647 |
| RsaNI | GTAC | 1 cut(s) | 646 |
| SaqAI | TTAA | 3 cut(s) | 339, 758, 843 |
| SatI | GCNGC | 3 cut(s) | 11, 63, 563 |
| Sau3AI | GATC | 6 cut(s) | 159, 204, 556, 598, 712, 721 |
| Sau96I | GGNCC | 5 cut(s) | 120, 242, 380, 399, 418 |
| SchI | GAGTC | 2 cut(s) | 357, 623 |
| ScrFI | CCNGG | 3 cut(s) | 167, 252, 516 |
| SduI | GDGCHC | 2 cut(s) | 145, 180 |
| SfaNI | GCATC | 1 cut(s) | 62 |
| SfcI | CTRYAG | 1 cut(s) | 806 |
| SinI | GGWCC | 2 cut(s) | 120, 242 |
| SmlI | CTYRAG | 2 cut(s) | 641, 842 |
| SmoI | CTYRAG | 2 cut(s) | 641, 842 |
| SphI | GCATGC | 1 cut(s) | 499 |
| SsiI | CCGC | 5 cut(s) | 13, 63, 248, 372, 487 |
| SspI | AATATT | 2 cut(s) | 762, 862 |
| SspMI | CTAG | 3 cut(s) | 441, 479, 786 |
| StyD4I | CCNGG | 3 cut(s) | 165, 250, 514 |
| StyI | CCWWGG | 2 cut(s) | 33, 181 |
| TaaI | ACNGT | 2 cut(s) | 308, 892 |
| TaiI | ACGT | 1 cut(s) | 538 |
| TaqI | TCGA | 1 cut(s) | 607 |
| TauI | GCSGC | 1 cut(s) | 65 |
| TfiI | GAWTC | 1 cut(s) | 102 |
| Tru1I | TTAA | 3 cut(s) | 339, 758, 843 |
| Tru9I | TTAA | 3 cut(s) | 339, 758, 843 |
| TscAI | CASTG | 2 cut(s) | 268, 703 |
| TseI | GCWGC | 2 cut(s) | 10, 562 |
| TspDTI | ATGAA | 4 cut(s) | 286, 542, 741, 882 |
| TspGWI | ACGGA | 1 cut(s) | 160 |
| TspRI | CASTG | 2 cut(s) | 268, 703 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 49, 251 |
| Vha464I | CTTAAG | 1 cut(s) | 842 |
| VpaK11BI | GGWCC | 2 cut(s) | 120, 242 |
| XceI | RCATGY | 1 cut(s) | 499 |
| XspI | CTAG | 3 cut(s) | 441, 479, 786 |
| Zsp2I | ATGCAT | 1 cut(s) | 501 |