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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 915 |
| Acc16I | TGCGCA | 1 cut(s) | 118 |
| AccB1I | GGYRCC | 1 cut(s) | 1006 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 951 |
| AccII | CGCG | 1 cut(s) | 46 |
| AciI | CCGC | 6 cut(s) | 28, 68, 149, 185, 231, 360 |
| AclWI | GGATC | 2 cut(s) | 278, 470 |
| AcsI | RAATTY | 4 cut(s) | 263, 299, 600, 798 |
| AcuI | CTGAAG | 2 cut(s) | 485, 1005 |
| AcvI | CACGTG | 1 cut(s) | 899 |
| AdeI | CACNNNGTG | 1 cut(s) | 899 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 27, 67, 490, 591, 947, 951 |
| AflII | CTTAAG | 1 cut(s) | 536 |
| AflIII | ACRYGT | 1 cut(s) | 862 |
| AgsI | TTSAA | 3 cut(s) | 275, 651, 712 |
| AjiI | CACGTC | 1 cut(s) | 863 |
| AleI | CACNNNNGTG | 1 cut(s) | 217 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 852, 884 |
| AluBI | AGCT | 2 cut(s) | 87, 718 |
| AluI | AGCT | 2 cut(s) | 87, 718 |
| AlwI | GGATC | 2 cut(s) | 278, 470 |
| AoxI | GGCC | 6 cut(s) | 29, 97, 779, 928, 937, 973 |
| ApeKI | GCWGC | 3 cut(s) | 91, 171, 176 |
| ApoI | RAATTY | 4 cut(s) | 263, 299, 600, 798 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 498 |
| AspLEI | GCGC | 5 cut(s) | 46, 48, 119, 176, 957 |
| AspS9I | GGNCC | 7 cut(s) | 30, 70, 422, 780, 928, 938, 974 |
| AsuC2I | CCSGG | 1 cut(s) | 96 |
| AsuHPI | GGTGA | 3 cut(s) | 835, 842, 1055 |
| AvaII | GGWCC | 2 cut(s) | 70, 422 |
| BaeGI | GKGCMC | 1 cut(s) | 209 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 314, 347, 682, 715 |
| BanI | GGYRCC | 1 cut(s) | 1006 |
| BbrPI | CACGTG | 1 cut(s) | 899 |
| BbsI | GAAGAC | 1 cut(s) | 632 |
| BbvCI | CCTCAGC | 1 cut(s) | 53 |
| BbvI | GCAGC | 3 cut(s) | 78, 183, 188 |
| BccI | CCATC | 4 cut(s) | 205, 398, 945, 956 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 96, 130 |
| BclI | TGATCA | 1 cut(s) | 720 |
| BcnI | CCSGG | 1 cut(s) | 96 |
| BfaI | CTAG | 7 cut(s) | 41, 132, 155, 480, 575, 662, 978 |
| BfrI | CTTAAG | 1 cut(s) | 536 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 368, 936 |
| BisI | GCNGC | 4 cut(s) | 29, 92, 172, 177 |
| BlsI | GCNGC | 4 cut(s) | 30, 93, 173, 178 |
| Bme1390I | CCNGG | 1 cut(s) | 96 |
| Bme18I | GGWCC | 2 cut(s) | 70, 422 |
| BmgBI | CACGTC | 1 cut(s) | 863 |
| BmgT120I | GGNCC | 7 cut(s) | 30, 70, 422, 780, 928, 938, 974 |
| BmiI | GGNNCC | 3 cut(s) | 32, 72, 1008 |
| BmrFI | CCNGG | 1 cut(s) | 96 |
| BmrI | ACTGGG | 3 cut(s) | 131, 196, 499 |
| BmsI | GCATC | 1 cut(s) | 520 |
| BmuI | ACTGGG | 3 cut(s) | 131, 196, 499 |
| BpiI | GAAGAC | 1 cut(s) | 632 |
| BpmI | CTGGAG | 1 cut(s) | 701 |
| Bpu10I | CCTNAGC | 1 cut(s) | 53 |
| BpuEI | CTTGAG | 1 cut(s) | 347 |
| BpuMI | CCSGG | 1 cut(s) | 96 |
| BsaAI | YACGTR | 2 cut(s) | 881, 899 |
| BsaJI | CCNNGG | 2 cut(s) | 804, 931 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 27, 67, 490, 591, 947, 951 |
| Bse1I | ACTGG | 4 cut(s) | 137, 202, 494, 670 |
| Bse3DI | GCAATG | 1 cut(s) | 381 |
| BseDI | CCNNGG | 2 cut(s) | 804, 931 |
| BseGI | GGATG | 4 cut(s) | 197, 512, 535, 956 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 27, 67, 490, 591, 947, 951 |
| BseMI | GCAATG | 1 cut(s) | 381 |
| BseMII | CTCAG | 3 cut(s) | 67, 330, 732 |
| BseNI | ACTGG | 4 cut(s) | 137, 202, 494, 670 |
| BsePI | GCGCGC | 1 cut(s) | 44 |
| BseSI | GKGCMC | 1 cut(s) | 209 |
| BseXI | GCAGC | 3 cut(s) | 78, 183, 188 |
| BseYI | CCCAGC | 2 cut(s) | 33, 159 |
| BsgI | GTGCAG | 1 cut(s) | 221 |
| Bsh1236I | CGCG | 1 cut(s) | 46 |
| BshFI | GGCC | 6 cut(s) | 31, 99, 781, 930, 939, 975 |
| BshNI | GGYRCC | 1 cut(s) | 1006 |
| BsiSI | CCGG | 1 cut(s) | 96 |
| BslFI | GGGAC | 3 cut(s) | 56, 555, 921 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 27, 67, 490, 591, 947, 951 |
| BsmFI | GGGAC | 3 cut(s) | 56, 555, 921 |
| BsnI | GGCC | 6 cut(s) | 31, 99, 781, 930, 939, 975 |
| Bsp1286I | GDGCHC | 1 cut(s) | 209 |
| Bsp143I | GATC | 3 cut(s) | 283, 475, 720 |
| Bsp19I | CCATGG | 1 cut(s) | 931 |
| BspACI | CCGC | 6 cut(s) | 28, 68, 149, 185, 231, 360 |
| BspANI | GGCC | 6 cut(s) | 31, 99, 781, 930, 939, 975 |
| BspCNI | CTCAG | 3 cut(s) | 66, 329, 733 |
| BspFNI | CGCG | 1 cut(s) | 46 |
| BspHI | TCATGA | 1 cut(s) | 559 |
| BspLI | GGNNCC | 3 cut(s) | 32, 72, 1008 |
| BspPI | GGATC | 2 cut(s) | 278, 470 |
| BspT107I | GGYRCC | 1 cut(s) | 1006 |
| BspTI | CTTAAG | 1 cut(s) | 536 |
| BsrDI | GCAATG | 1 cut(s) | 381 |
| BsrI | ACTGG | 4 cut(s) | 137, 202, 494, 670 |
| BssECI | CCNNGG | 2 cut(s) | 804, 931 |
| BssHII | GCGCGC | 1 cut(s) | 44 |
| BssMI | GATC | 3 cut(s) | 283, 475, 720 |
| BssT1I | CCWWGG | 2 cut(s) | 804, 931 |
| Bst4CI | ACNGT | 2 cut(s) | 9, 426 |
| Bst6I | CTCTTC | 1 cut(s) | 276 |
| BstAFI | CTTAAG | 1 cut(s) | 536 |
| BstBAI | YACGTR | 2 cut(s) | 881, 899 |
| BstC8I | GCNNGC | 8 cut(s) | 26, 46, 66, 89, 121, 441, 608, 973 |
| BstDEI | CTNAG | 5 cut(s) | 53, 316, 461, 741, 1011 |
| BstDSI | CCRYGG | 1 cut(s) | 931 |
| BstF5I | GGATG | 4 cut(s) | 197, 512, 535, 956 |
| BstFNI | CGCG | 1 cut(s) | 46 |
| BstHHI | GCGC | 5 cut(s) | 46, 48, 119, 176, 957 |
| BstKTI | GATC | 3 cut(s) | 286, 478, 723 |
| BstMBI | GATC | 3 cut(s) | 283, 475, 720 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 34, 43, 54, 148, 213, 368, 936 |
| BstNSI | RCATGY | 1 cut(s) | 68 |
| BstSCI | CCNGG | 1 cut(s) | 94 |
| BstSLI | GKGCMC | 1 cut(s) | 209 |
| BstUI | CGCG | 1 cut(s) | 46 |
| BstV1I | GCAGC | 3 cut(s) | 78, 183, 188 |
| BstV2I | GAAGAC | 1 cut(s) | 632 |
| BsuRI | GGCC | 6 cut(s) | 31, 99, 781, 930, 939, 975 |
| BtgI | CCRYGG | 1 cut(s) | 931 |
| BtrI | CACGTC | 1 cut(s) | 863 |
| BtsCI | GGATG | 4 cut(s) | 197, 512, 535, 956 |
| BtsI | GCAGTG | 1 cut(s) | 243 |
| BtsIMutI | CAGTG | 5 cut(s) | 144, 209, 243, 487, 596 |
| Cac8I | GCNNGC | 8 cut(s) | 26, 46, 66, 89, 121, 441, 608, 973 |
| CciI | TCATGA | 1 cut(s) | 559 |
| CfoI | GCGC | 5 cut(s) | 46, 48, 119, 176, 957 |
| Cfr13I | GGNCC | 7 cut(s) | 30, 70, 422, 780, 928, 938, 974 |
| CseI | GACGC | 1 cut(s) | 552 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 170, 205 |
| CviAII | CATG | 5 cut(s) | 65, 560, 932, 986, 1053 |
| DdeI | CTNAG | 5 cut(s) | 53, 316, 461, 741, 1011 |
| DpnI | GATC | 3 cut(s) | 285, 477, 722 |
| DpnII | GATC | 3 cut(s) | 283, 475, 720 |
| DraIII | CACNNNGTG | 1 cut(s) | 899 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 915 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 915 |
| Eam1104I | CTCTTC | 1 cut(s) | 276 |
| EarI | CTCTTC | 1 cut(s) | 276 |
| EciI | GGCGGA | 1 cut(s) | 138 |
| Eco130I | CCWWGG | 2 cut(s) | 804, 931 |
| Eco47I | GGWCC | 2 cut(s) | 70, 422 |
| Eco57I | CTGAAG | 2 cut(s) | 485, 1005 |
| Eco72I | CACGTG | 1 cut(s) | 899 |
| EcoO109I | RGGNCCY | 1 cut(s) | 938 |
| EcoT14I | CCWWGG | 2 cut(s) | 804, 931 |
| ErhI | CCWWGG | 2 cut(s) | 804, 931 |
| FaeI | CATG | 5 cut(s) | 68, 563, 935, 989, 1056 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 530, 562 |
| FaqI | GGGAC | 3 cut(s) | 56, 555, 921 |
| FatI | CATG | 5 cut(s) | 64, 559, 931, 985, 1052 |
| FauI | CCCGC | 2 cut(s) | 192, 238 |
| FbaI | TGATCA | 1 cut(s) | 720 |
| Fnu4HI | GCNGC | 4 cut(s) | 29, 92, 172, 177 |
| FokI | GGATG | 4 cut(s) | 184, 499, 542, 963 |
| Fsp4HI | GCNGC | 4 cut(s) | 29, 92, 172, 177 |
| FspBI | CTAG | 7 cut(s) | 41, 132, 155, 480, 575, 662, 978 |
| FspI | TGCGCA | 1 cut(s) | 118 |
| GlaI | GCGC | 5 cut(s) | 45, 47, 118, 175, 956 |
| GluI | GCNGC | 4 cut(s) | 29, 92, 172, 177 |
| GsaI | CCCAGC | 2 cut(s) | 37, 163 |
| GsuI | CTGGAG | 1 cut(s) | 701 |
| HaeIII | GGCC | 6 cut(s) | 31, 99, 781, 930, 939, 975 |
| HapII | CCGG | 1 cut(s) | 96 |
| HgaI | GACGC | 1 cut(s) | 552 |
| HhaI | GCGC | 5 cut(s) | 46, 48, 119, 176, 957 |
| Hin1II | CATG | 5 cut(s) | 68, 563, 935, 989, 1056 |
| Hin6I | GCGC | 5 cut(s) | 44, 46, 117, 174, 955 |
| HinP1I | GCGC | 5 cut(s) | 44, 46, 117, 174, 955 |
| HinfI | GANTC | 3 cut(s) | 314, 494, 628 |
| HpaII | CCGG | 1 cut(s) | 96 |
| HphI | GGTGA | 3 cut(s) | 835, 842, 1055 |
| Hpy188I | TCNGA | 2 cut(s) | 319, 349 |
| Hpy188III | TCNNGA | 1 cut(s) | 560 |
| HpyAV | CCTTC | 3 cut(s) | 314, 509, 1029 |
| HpyCH4III | ACNGT | 2 cut(s) | 9, 426 |
| HpyCH4IV | ACGT | 3 cut(s) | 862, 880, 898 |
| HpyCH4V | TGCA | 9 cut(s) | 104, 142, 171, 238, 439, 733, 848, 893, 1000 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 34, 43, 54, 148, 213, 368, 936 |
| HpyF3I | CTNAG | 5 cut(s) | 53, 316, 461, 741, 1011 |
| HpySE526I | ACGT | 3 cut(s) | 862, 880, 898 |
| Hsp92II | CATG | 5 cut(s) | 68, 563, 935, 989, 1056 |
| HspAI | GCGC | 5 cut(s) | 44, 46, 117, 174, 955 |
| Ksp22I | TGATCA | 1 cut(s) | 720 |
| Kzo9I | GATC | 3 cut(s) | 283, 475, 720 |
| LmnI | GCTCC | 1 cut(s) | 576 |
| Lsp1109I | GCAGC | 3 cut(s) | 78, 183, 188 |
| LweI | GCATC | 1 cut(s) | 520 |
| MaeI | CTAG | 7 cut(s) | 41, 132, 155, 480, 575, 662, 978 |
| MaeII | ACGT | 3 cut(s) | 862, 880, 898 |
| MaeIII | GTNAC | 2 cut(s) | 221, 394 |
| MalI | GATC | 3 cut(s) | 285, 477, 722 |
| MboI | GATC | 3 cut(s) | 283, 475, 720 |
| MboII | GAAGA | 2 cut(s) | 263, 637 |
| MhlI | GDGCHC | 1 cut(s) | 209 |
| MluCI | AATT | 6 cut(s) | 263, 299, 600, 798, 811, 1068 |
| MlyI | GAGTC | 2 cut(s) | 308, 622 |
| MmeI | TCCRAC | 2 cut(s) | 311, 838 |
| MroXI | GAANNNNTTC | 1 cut(s) | 498 |
| MseI | TTAA | 3 cut(s) | 452, 506, 537 |
| MslI | CAYNNNNRTG | 3 cut(s) | 202, 217, 339 |
| MspCI | CTTAAG | 1 cut(s) | 536 |
| MspI | CCGG | 1 cut(s) | 96 |
| MspR9I | CCNGG | 1 cut(s) | 96 |
| MvnI | CGCG | 1 cut(s) | 46 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 34, 43, 54, 148, 213, 368, 936 |
| NciI | CCSGG | 1 cut(s) | 96 |
| NcoI | CCATGG | 1 cut(s) | 931 |
| NdeII | GATC | 3 cut(s) | 283, 475, 720 |
| NlaIII | CATG | 5 cut(s) | 68, 563, 935, 989, 1056 |
| NlaIV | GGNNCC | 3 cut(s) | 32, 72, 1008 |
| NsbI | TGCGCA | 1 cut(s) | 118 |
| NspI | RCATGY | 1 cut(s) | 68 |
| OliI | CACNNNNGTG | 1 cut(s) | 217 |
| PaeI | GCATGC | 1 cut(s) | 68 |
| PagI | TCATGA | 1 cut(s) | 559 |
| PauI | GCGCGC | 1 cut(s) | 44 |
| PdmI | GAANNNNTTC | 1 cut(s) | 498 |
| PfeI | GAWTC | 1 cut(s) | 494 |
| PflFI | GACNNNGTC | 1 cut(s) | 7 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 951 |
| PkrI | GCNGC | 4 cut(s) | 30, 93, 173, 178 |
| PleI | GAGTC | 2 cut(s) | 308, 622 |
| PmaCI | CACGTG | 1 cut(s) | 899 |
| PmlI | CACGTG | 1 cut(s) | 899 |
| PpsI | GAGTC | 2 cut(s) | 308, 622 |
| Ppu21I | YACGTR | 2 cut(s) | 881, 899 |
| PspCI | CACGTG | 1 cut(s) | 899 |
| PspFI | CCCAGC | 2 cut(s) | 33, 159 |
| PspN4I | GGNNCC | 3 cut(s) | 32, 72, 1008 |
| PspPI | GGNCC | 7 cut(s) | 30, 70, 422, 780, 928, 938, 974 |
| PsyI | GACNNNGTC | 1 cut(s) | 7 |
| PteI | GCGCGC | 1 cut(s) | 44 |
| RseI | CAYNNNNRTG | 3 cut(s) | 202, 217, 339 |
| SaqAI | TTAA | 3 cut(s) | 452, 506, 537 |
| SatI | GCNGC | 4 cut(s) | 29, 92, 172, 177 |
| Sau3AI | GATC | 3 cut(s) | 283, 475, 720 |
| Sau96I | GGNCC | 7 cut(s) | 30, 70, 422, 780, 928, 938, 974 |
| SchI | GAGTC | 2 cut(s) | 308, 622 |
| ScrFI | CCNGG | 1 cut(s) | 96 |
| SduI | GDGCHC | 1 cut(s) | 209 |
| SfaNI | GCATC | 1 cut(s) | 520 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 936 |
| SinI | GGWCC | 2 cut(s) | 70, 422 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 202, 217, 339 |
| SmlI | CTYRAG | 2 cut(s) | 326, 536 |
| SmoI | CTYRAG | 2 cut(s) | 326, 536 |
| SphI | GCATGC | 1 cut(s) | 68 |
| Sse9I | AATT | 6 cut(s) | 263, 299, 600, 798, 811, 1068 |
| SsiI | CCGC | 6 cut(s) | 28, 68, 149, 185, 231, 360 |
| SspMI | CTAG | 7 cut(s) | 41, 132, 155, 480, 575, 662, 978 |
| StyD4I | CCNGG | 1 cut(s) | 94 |
| StyI | CCWWGG | 2 cut(s) | 804, 931 |
| TaaI | ACNGT | 2 cut(s) | 9, 426 |
| TaiI | ACGT | 3 cut(s) | 865, 883, 901 |
| TaqI | TCGA | 1 cut(s) | 292 |
| TasI | AATT | 6 cut(s) | 263, 299, 600, 798, 811, 1068 |
| TauI | GCSGC | 1 cut(s) | 31 |
| TfiI | GAWTC | 1 cut(s) | 494 |
| Tru1I | TTAA | 3 cut(s) | 452, 506, 537 |
| Tru9I | TTAA | 3 cut(s) | 452, 506, 537 |
| TscAI | CASTG | 5 cut(s) | 144, 209, 250, 494, 596 |
| TseI | GCWGC | 3 cut(s) | 91, 171, 176 |
| TspDTI | ATGAA | 3 cut(s) | 548, 612, 1062 |
| TspRI | CASTG | 5 cut(s) | 144, 209, 250, 494, 596 |
| Tth111I | GACNNNGTC | 1 cut(s) | 7 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 951 |
| Vha464I | CTTAAG | 1 cut(s) | 536 |
| VpaK11BI | GGWCC | 2 cut(s) | 70, 422 |
| XapI | RAATTY | 4 cut(s) | 263, 299, 600, 798 |
| XceI | RCATGY | 1 cut(s) | 68 |
| XmnI | GAANNNNTTC | 1 cut(s) | 498 |
| XspI | CTAG | 7 cut(s) | 41, 132, 155, 480, 575, 662, 978 |