No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 411 |
| AccB1I | GGYRCC | 2 cut(s) | 550, 662 |
| AccII | CGCG | 1 cut(s) | 778 |
| AciI | CCGC | 1 cut(s) | 881 |
| AclWI | GGATC | 2 cut(s) | 157, 802 |
| AcsI | RAATTY | 2 cut(s) | 342, 731 |
| AcuI | CTGAAG | 2 cut(s) | 549, 869 |
| AfaI | GTAC | 1 cut(s) | 495 |
| AfeI | AGCGCT | 1 cut(s) | 860 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 48, 876, 887 |
| AflIII | ACRYGT | 1 cut(s) | 102 |
| AgsI | TTSAA | 5 cut(s) | 94, 256, 310, 699, 784 |
| AjiI | CACGTC | 1 cut(s) | 122 |
| AjnI | CCWGG | 1 cut(s) | 320 |
| AleI | CACNNNNGTG | 1 cut(s) | 821 |
| AluBI | AGCT | 3 cut(s) | 262, 284, 849 |
| AluI | AGCT | 3 cut(s) | 262, 284, 849 |
| Alw26I | GTCTC | 1 cut(s) | 128 |
| AlwI | GGATC | 2 cut(s) | 157, 802 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 132 |
| Aor51HI | AGCGCT | 1 cut(s) | 860 |
| AoxI | GGCC | 7 cut(s) | 323, 472, 481, 517, 720, 867, 878 |
| ApeKI | GCWGC | 1 cut(s) | 861 |
| ApoI | RAATTY | 2 cut(s) | 342, 731 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 106, 138 |
| AspLEI | GCGC | 1 cut(s) | 861 |
| AspS9I | GGNCC | 5 cut(s) | 413, 472, 482, 518, 721 |
| AsuHPI | GGTGA | 7 cut(s) | 524, 668, 676, 683, 866, 890, 918 |
| AvaII | GGWCC | 1 cut(s) | 413 |
| BanI | GGYRCC | 2 cut(s) | 550, 662 |
| BbsI | GAAGAC | 1 cut(s) | 176 |
| BbvI | GCAGC | 1 cut(s) | 848 |
| BccI | CCATC | 6 cut(s) | 149, 509, 635, 653, 657, 839 |
| BceAI | ACGGC | 2 cut(s) | 459, 512 |
| BciT130I | CCWGG | 1 cut(s) | 322 |
| BclI | TGATCA | 2 cut(s) | 264, 904 |
| BcoDI | GTCTC | 1 cut(s) | 128 |
| BfaI | CTAG | 2 cut(s) | 206, 522 |
| BfmI | CTRYAG | 1 cut(s) | 601 |
| BfoI | RGCGCY | 1 cut(s) | 862 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 480 |
| BisI | GCNGC | 2 cut(s) | 862, 881 |
| BlsI | GCNGC | 2 cut(s) | 863, 882 |
| Bme1390I | CCNGG | 1 cut(s) | 322 |
| Bme18I | GGWCC | 1 cut(s) | 413 |
| BmgBI | CACGTC | 1 cut(s) | 122 |
| BmgT120I | GGNCC | 5 cut(s) | 413, 472, 482, 518, 721 |
| BmiI | GGNNCC | 3 cut(s) | 415, 552, 664 |
| BmrFI | CCNGG | 1 cut(s) | 322 |
| BmsI | GCATC | 1 cut(s) | 654 |
| BpiI | GAAGAC | 1 cut(s) | 176 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 471, 503 |
| Bpu10I | CCTNAGC | 1 cut(s) | 193 |
| BsaJI | CCNNGG | 3 cut(s) | 348, 475, 667 |
| BsaWI | WCCGGW | 1 cut(s) | 921 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 48, 876, 887 |
| Bse1I | ACTGG | 1 cut(s) | 214 |
| Bse3DI | GCAATG | 1 cut(s) | 595 |
| BseBI | CCWGG | 1 cut(s) | 322 |
| BseDI | CCNNGG | 3 cut(s) | 348, 475, 667 |
| BseGI | GGATG | 2 cut(s) | 645, 646 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 48, 876, 887 |
| BseMI | GCAATG | 1 cut(s) | 595 |
| BseMII | CTCAG | 2 cut(s) | 139, 276 |
| BseNI | ACTGG | 1 cut(s) | 214 |
| BseXI | GCAGC | 1 cut(s) | 848 |
| Bsh1236I | CGCG | 1 cut(s) | 778 |
| BshFI | GGCC | 7 cut(s) | 325, 474, 483, 519, 722, 869, 880 |
| BshNI | GGYRCC | 2 cut(s) | 550, 662 |
| BsiSI | CCGG | 1 cut(s) | 922 |
| BslFI | GGGAC | 5 cut(s) | 399, 422, 465, 856, 903 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 48, 876, 887 |
| BsmAI | GTCTC | 1 cut(s) | 128 |
| BsmBI | CGTCTC | 1 cut(s) | 128 |
| BsmFI | GGGAC | 5 cut(s) | 399, 422, 465, 856, 903 |
| BsnI | GGCC | 7 cut(s) | 325, 474, 483, 519, 722, 869, 880 |
| Bsp143I | GATC | 5 cut(s) | 162, 264, 807, 895, 904 |
| BspACI | CCGC | 1 cut(s) | 881 |
| BspANI | GGCC | 7 cut(s) | 325, 474, 483, 519, 722, 869, 880 |
| BspCNI | CTCAG | 2 cut(s) | 138, 277 |
| BspFNI | CGCG | 1 cut(s) | 778 |
| BspHI | TCATGA | 2 cut(s) | 16, 804 |
| BspLI | GGNNCC | 3 cut(s) | 415, 552, 664 |
| BspPI | GGATC | 2 cut(s) | 157, 802 |
| BspT107I | GGYRCC | 2 cut(s) | 550, 662 |
| BsrDI | GCAATG | 1 cut(s) | 595 |
| BsrI | ACTGG | 1 cut(s) | 214 |
| BssECI | CCNNGG | 3 cut(s) | 348, 475, 667 |
| BssMI | GATC | 5 cut(s) | 162, 264, 807, 895, 904 |
| BssT1I | CCWWGG | 1 cut(s) | 667 |
| Bst2UI | CCWGG | 1 cut(s) | 322 |
| Bst4CI | ACNGT | 2 cut(s) | 43, 618 |
| Bst6I | CTCTTC | 2 cut(s) | 801, 869 |
| BstDEI | CTNAG | 4 cut(s) | 125, 193, 285, 555 |
| BstDSI | CCRYGG | 1 cut(s) | 475 |
| BstEII | GGTNACC | 1 cut(s) | 924 |
| BstF5I | GGATG | 2 cut(s) | 645, 646 |
| BstFNI | CGCG | 1 cut(s) | 778 |
| BstH2I | RGCGCY | 1 cut(s) | 862 |
| BstHHI | GCGC | 1 cut(s) | 861 |
| BstKTI | GATC | 5 cut(s) | 165, 267, 810, 898, 907 |
| BstMAI | GTCTC | 1 cut(s) | 128 |
| BstMBI | GATC | 5 cut(s) | 162, 264, 807, 895, 904 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 480, 752 |
| BstNI | CCWGG | 1 cut(s) | 322 |
| BstNSI | RCATGY | 1 cut(s) | 106 |
| BstPI | GGTNACC | 1 cut(s) | 924 |
| BstSCI | CCNGG | 1 cut(s) | 320 |
| BstSFI | CTRYAG | 1 cut(s) | 601 |
| BstUI | CGCG | 1 cut(s) | 778 |
| BstV1I | GCAGC | 1 cut(s) | 848 |
| BstV2I | GAAGAC | 1 cut(s) | 176 |
| BsuRI | GGCC | 7 cut(s) | 325, 474, 483, 519, 722, 869, 880 |
| BtgI | CCRYGG | 1 cut(s) | 475 |
| BtrI | CACGTC | 1 cut(s) | 122 |
| BtsCI | GGATG | 2 cut(s) | 645, 646 |
| BtsI | GCAGTG | 1 cut(s) | 315 |
| BtsIMutI | CAGTG | 2 cut(s) | 53, 315 |
| CaiI | CAGNNNCTG | 1 cut(s) | 132 |
| CciI | TCATGA | 2 cut(s) | 16, 804 |
| CfoI | GCGC | 1 cut(s) | 861 |
| Cfr13I | GGNCC | 5 cut(s) | 413, 472, 482, 518, 721 |
| Csp6I | GTAC | 1 cut(s) | 494 |
| CviQI | GTAC | 1 cut(s) | 494 |
| DdeI | CTNAG | 4 cut(s) | 125, 193, 285, 555 |
| DpnI | GATC | 5 cut(s) | 164, 266, 809, 897, 906 |
| DpnII | GATC | 5 cut(s) | 162, 264, 807, 895, 904 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 411 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 411 |
| Eam1104I | CTCTTC | 2 cut(s) | 801, 869 |
| EarI | CTCTTC | 2 cut(s) | 801, 869 |
| Eco130I | CCWWGG | 1 cut(s) | 667 |
| Eco147I | AGGCCT | 1 cut(s) | 325 |
| Eco47I | GGWCC | 1 cut(s) | 413 |
| Eco47III | AGCGCT | 1 cut(s) | 860 |
| Eco57I | CTGAAG | 2 cut(s) | 549, 869 |
| Eco91I | GGTNACC | 1 cut(s) | 924 |
| EcoO109I | RGGNCCY | 1 cut(s) | 482 |
| EcoO65I | GGTNACC | 1 cut(s) | 924 |
| EcoRII | CCWGG | 1 cut(s) | 320 |
| EcoT14I | CCWWGG | 1 cut(s) | 667 |
| ErhI | CCWWGG | 1 cut(s) | 667 |
| Esp3I | CGTCTC | 1 cut(s) | 128 |
| FaqI | GGGAC | 5 cut(s) | 399, 422, 465, 856, 903 |
| FbaI | TGATCA | 2 cut(s) | 264, 904 |
| Fnu4HI | GCNGC | 2 cut(s) | 862, 881 |
| FokI | GGATG | 2 cut(s) | 632, 653 |
| Fsp4HI | GCNGC | 2 cut(s) | 862, 881 |
| FspBI | CTAG | 2 cut(s) | 206, 522 |
| GlaI | GCGC | 1 cut(s) | 860 |
| GluI | GCNGC | 2 cut(s) | 862, 881 |
| HaeII | RGCGCY | 1 cut(s) | 862 |
| HaeIII | GGCC | 7 cut(s) | 325, 474, 483, 519, 722, 869, 880 |
| HapII | CCGG | 1 cut(s) | 922 |
| HhaI | GCGC | 1 cut(s) | 861 |
| Hin6I | GCGC | 1 cut(s) | 859 |
| HinP1I | GCGC | 1 cut(s) | 859 |
| HinfI | GANTC | 5 cut(s) | 172, 243, 780, 910, 918 |
| HpaII | CCGG | 1 cut(s) | 922 |
| HphI | GGTGA | 7 cut(s) | 524, 668, 676, 683, 866, 890, 918 |
| Hpy188I | TCNGA | 2 cut(s) | 349, 909 |
| Hpy188III | TCNNGA | 3 cut(s) | 17, 166, 805 |
| HpyAV | CCTTC | 4 cut(s) | 132, 262, 500, 573 |
| HpyCH4III | ACNGT | 2 cut(s) | 43, 618 |
| HpyCH4IV | ACGT | 4 cut(s) | 121, 406, 714, 766 |
| HpyCH4V | TGCA | 5 cut(s) | 277, 392, 544, 680, 864 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 480, 752 |
| HpyF3I | CTNAG | 4 cut(s) | 125, 193, 285, 555 |
| HpySE526I | ACGT | 4 cut(s) | 121, 406, 714, 766 |
| HspAI | GCGC | 1 cut(s) | 859 |
| Ksp22I | TGATCA | 2 cut(s) | 264, 904 |
| Kzo9I | GATC | 5 cut(s) | 162, 264, 807, 895, 904 |
| LmnI | GCTCC | 1 cut(s) | 33 |
| Lsp1109I | GCAGC | 1 cut(s) | 848 |
| LweI | GCATC | 1 cut(s) | 654 |
| MaeI | CTAG | 2 cut(s) | 206, 522 |
| MaeII | ACGT | 4 cut(s) | 121, 406, 714, 766 |
| MaeIII | GTNAC | 2 cut(s) | 767, 924 |
| MalI | GATC | 5 cut(s) | 164, 266, 809, 897, 906 |
| MboI | GATC | 5 cut(s) | 162, 264, 807, 895, 904 |
| MboII | GAAGA | 4 cut(s) | 164, 181, 788, 886 |
| MluCI | AATT | 3 cut(s) | 342, 355, 731 |
| MlyI | GAGTC | 2 cut(s) | 166, 919 |
| MmeI | TCCRAC | 2 cut(s) | 204, 382 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 3 cut(s) | 654, 657, 821 |
| MspI | CCGG | 1 cut(s) | 922 |
| MspR9I | CCNGG | 1 cut(s) | 322 |
| MvaI | CCWGG | 1 cut(s) | 322 |
| MvnI | CGCG | 1 cut(s) | 778 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 480, 752 |
| NdeII | GATC | 5 cut(s) | 162, 264, 807, 895, 904 |
| NlaIV | GGNNCC | 3 cut(s) | 415, 552, 664 |
| NmeAIII | GCCGAG | 1 cut(s) | 895 |
| NmuCI | GTSAC | 1 cut(s) | 924 |
| NspI | RCATGY | 1 cut(s) | 106 |
| OliI | CACNNNNGTG | 1 cut(s) | 821 |
| PagI | TCATGA | 2 cut(s) | 16, 804 |
| PceI | AGGCCT | 1 cut(s) | 325 |
| PciI | ACATGT | 1 cut(s) | 102 |
| PfeI | GAWTC | 3 cut(s) | 243, 780, 918 |
| PkrI | GCNGC | 2 cut(s) | 863, 882 |
| PleI | GAGTC | 2 cut(s) | 166, 918 |
| PpsI | GAGTC | 2 cut(s) | 166, 918 |
| PscI | ACATGT | 1 cut(s) | 102 |
| Psp6I | CCWGG | 1 cut(s) | 320 |
| PspEI | GGTNACC | 1 cut(s) | 924 |
| PspGI | CCWGG | 1 cut(s) | 320 |
| PspN4I | GGNNCC | 3 cut(s) | 415, 552, 664 |
| PspPI | GGNCC | 5 cut(s) | 413, 472, 482, 518, 721 |
| PstNI | CAGNNNCTG | 1 cut(s) | 132 |
| RsaI | GTAC | 1 cut(s) | 495 |
| RsaNI | GTAC | 1 cut(s) | 494 |
| RseI | CAYNNNNRTG | 3 cut(s) | 654, 657, 821 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 2 cut(s) | 862, 881 |
| Sau3AI | GATC | 5 cut(s) | 162, 264, 807, 895, 904 |
| Sau96I | GGNCC | 5 cut(s) | 413, 472, 482, 518, 721 |
| SchI | GAGTC | 2 cut(s) | 166, 919 |
| ScrFI | CCNGG | 1 cut(s) | 322 |
| SfaNI | GCATC | 1 cut(s) | 654 |
| SfcI | CTRYAG | 1 cut(s) | 601 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 480 |
| SinI | GGWCC | 1 cut(s) | 413 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 654, 657, 821 |
| Sse9I | AATT | 3 cut(s) | 342, 355, 731 |
| SseBI | AGGCCT | 1 cut(s) | 325 |
| SsiI | CCGC | 1 cut(s) | 881 |
| SspMI | CTAG | 2 cut(s) | 206, 522 |
| StuI | AGGCCT | 1 cut(s) | 325 |
| StyD4I | CCNGG | 1 cut(s) | 320 |
| StyI | CCWWGG | 1 cut(s) | 667 |
| TaaI | ACNGT | 2 cut(s) | 43, 618 |
| TaiI | ACGT | 4 cut(s) | 124, 409, 717, 769 |
| TaqI | TCGA | 1 cut(s) | 834 |
| TasI | AATT | 3 cut(s) | 342, 355, 731 |
| TauI | GCSGC | 1 cut(s) | 883 |
| TfiI | GAWTC | 3 cut(s) | 243, 780, 918 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 2 cut(s) | 53, 322 |
| TseFI | GTSAC | 1 cut(s) | 924 |
| TseI | GCWGC | 1 cut(s) | 861 |
| Tsp45I | GTSAC | 1 cut(s) | 924 |
| TspDTI | ATGAA | 3 cut(s) | 5, 69, 606 |
| TspRI | CASTG | 2 cut(s) | 53, 322 |
| VpaK11BI | GGWCC | 1 cut(s) | 413 |
| XapI | RAATTY | 2 cut(s) | 342, 731 |
| XceI | RCATGY | 1 cut(s) | 106 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 634, 656 |
| XspI | CTAG | 2 cut(s) | 206, 522 |