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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 3 cut(s) | 243, 346, 403 |
| AclWI | GGATC | 3 cut(s) | 430, 572, 755 |
| AcoI | YGGCCR | 1 cut(s) | 16 |
| AcsI | RAATTY | 1 cut(s) | 593 |
| AcyI | GRCGYC | 1 cut(s) | 282 |
| AfaI | GTAC | 2 cut(s) | 137, 626 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 225, 291, 634, 775, 876 |
| AflII | CTTAAG | 1 cut(s) | 821 |
| AgsI | TTSAA | 2 cut(s) | 166, 793 |
| AjnI | CCWGG | 1 cut(s) | 224 |
| AluBI | AGCT | 1 cut(s) | 40 |
| AluI | AGCT | 1 cut(s) | 40 |
| AlwI | GGATC | 3 cut(s) | 430, 572, 755 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 475 |
| AoxI | GGCC | 6 cut(s) | 16, 196, 248, 293, 307, 465 |
| ApeKI | GCWGC | 8 cut(s) | 233, 348, 371, 374, 459, 478, 494, 497 |
| ApoI | RAATTY | 1 cut(s) | 593 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 783 |
| AspLEI | GCGC | 6 cut(s) | 182, 304, 346, 348, 405, 415 |
| AspS9I | GGNCC | 3 cut(s) | 197, 294, 466 |
| AsuC2I | CCSGG | 1 cut(s) | 417 |
| AsuHPI | GGTGA | 2 cut(s) | 195, 733 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 608, 641 |
| BbsI | GAAGAC | 2 cut(s) | 180, 800 |
| BbvI | GCAGC | 8 cut(s) | 245, 335, 358, 361, 465, 471, 506, 509 |
| BccI | CCATC | 3 cut(s) | 8, 293, 718 |
| BceAI | ACGGC | 6 cut(s) | 43, 193, 263, 308, 322, 480 |
| BciT130I | CCWGG | 1 cut(s) | 226 |
| BcnI | CCSGG | 1 cut(s) | 417 |
| BfaI | CTAG | 3 cut(s) | 410, 429, 522 |
| BfoI | RGCGCY | 2 cut(s) | 183, 416 |
| BfrI | CTTAAG | 1 cut(s) | 821 |
| BlpI | GCTNAGC | 2 cut(s) | 41, 340 |
| Bme1390I | CCNGG | 2 cut(s) | 226, 417 |
| BmgT120I | GGNCC | 3 cut(s) | 197, 294, 466 |
| BmiI | GGNNCC | 4 cut(s) | 10, 57, 193, 199 |
| BmrFI | CCNGG | 2 cut(s) | 226, 417 |
| BmsI | GCATC | 1 cut(s) | 805 |
| BpiI | GAAGAC | 2 cut(s) | 180, 800 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 41, 340 |
| BpuEI | CTTGAG | 1 cut(s) | 641 |
| BpuMI | CCSGG | 1 cut(s) | 417 |
| Bsa29I | ATCGAT | 2 cut(s) | 360, 434 |
| BsaHI | GRCGYC | 1 cut(s) | 282 |
| BsaJI | CCNNGG | 2 cut(s) | 19, 241 |
| BsaWI | WCCGGW | 1 cut(s) | 277 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 62, 92 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 225, 291, 634, 775, 876 |
| Bse3DI | GCAATG | 1 cut(s) | 851 |
| BseBI | CCWGG | 1 cut(s) | 226 |
| BseCI | ATCGAT | 2 cut(s) | 360, 434 |
| BseDI | CCNNGG | 2 cut(s) | 19, 241 |
| BseGI | GGATG | 2 cut(s) | 285, 820 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 225, 291, 634, 775, 876 |
| BseMI | GCAATG | 1 cut(s) | 851 |
| BseMII | CTCAG | 4 cut(s) | 32, 354, 624, 737 |
| BsePI | GCGCGC | 1 cut(s) | 344 |
| BseXI | GCAGC | 8 cut(s) | 245, 335, 358, 361, 465, 471, 506, 509 |
| BseYI | CCCAGC | 3 cut(s) | 210, 468, 774 |
| BsgI | GTGCAG | 1 cut(s) | 100 |
| Bsh1236I | CGCG | 3 cut(s) | 243, 346, 403 |
| BshFI | GGCC | 6 cut(s) | 18, 198, 250, 295, 309, 467 |
| BshVI | ATCGAT | 2 cut(s) | 360, 434 |
| BsiSI | CCGG | 5 cut(s) | 195, 278, 417, 442, 454 |
| BslFI | GGGAC | 1 cut(s) | 840 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 225, 291, 634, 775, 876 |
| BsmFI | GGGAC | 1 cut(s) | 840 |
| BsnI | GGCC | 6 cut(s) | 18, 198, 250, 295, 309, 467 |
| Bsp1407I | TGTACA | 1 cut(s) | 135 |
| Bsp143I | GATC | 6 cut(s) | 267, 361, 431, 435, 577, 760 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 41, 340 |
| BspANI | GGCC | 6 cut(s) | 18, 198, 250, 295, 309, 467 |
| BspCNI | CTCAG | 4 cut(s) | 33, 353, 623, 738 |
| BspDI | ATCGAT | 2 cut(s) | 360, 434 |
| BspFNI | CGCG | 3 cut(s) | 243, 346, 403 |
| BspHI | TCATGA | 1 cut(s) | 844 |
| BspLI | GGNNCC | 4 cut(s) | 10, 57, 193, 199 |
| BspPI | GGATC | 3 cut(s) | 430, 572, 755 |
| BspQI | GCTCTTC | 1 cut(s) | 397 |
| BspTI | CTTAAG | 1 cut(s) | 821 |
| BsrDI | GCAATG | 1 cut(s) | 851 |
| BsrGI | TGTACA | 1 cut(s) | 135 |
| BssECI | CCNNGG | 2 cut(s) | 19, 241 |
| BssHII | GCGCGC | 1 cut(s) | 344 |
| BssMI | GATC | 6 cut(s) | 267, 361, 431, 435, 577, 760 |
| BssNI | GRCGYC | 1 cut(s) | 282 |
| Bst2UI | CCWGG | 1 cut(s) | 226 |
| Bst4CI | ACNGT | 4 cut(s) | 140, 632, 810, 871 |
| Bst6I | CTCTTC | 2 cut(s) | 397, 549 |
| BstACI | GRCGYC | 1 cut(s) | 282 |
| BstAFI | CTTAAG | 1 cut(s) | 821 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 101 |
| BstAUI | TGTACA | 1 cut(s) | 135 |
| BstC8I | GCNNGC | 5 cut(s) | 115, 216, 346, 385, 893 |
| BstDEI | CTNAG | 4 cut(s) | 41, 340, 610, 746 |
| BstDSI | CCRYGG | 1 cut(s) | 241 |
| BstF5I | GGATG | 2 cut(s) | 285, 820 |
| BstFNI | CGCG | 3 cut(s) | 243, 346, 403 |
| BstH2I | RGCGCY | 2 cut(s) | 183, 416 |
| BstHHI | GCGC | 6 cut(s) | 182, 304, 346, 348, 405, 415 |
| BstKTI | GATC | 6 cut(s) | 270, 364, 434, 438, 580, 763 |
| BstMBI | GATC | 6 cut(s) | 267, 361, 431, 435, 577, 760 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 37, 101, 110, 215, 301, 345, 380, 459, 478 |
| BstNI | CCWGG | 1 cut(s) | 226 |
| BstSCI | CCNGG | 2 cut(s) | 224, 415 |
| BstUI | CGCG | 3 cut(s) | 243, 346, 403 |
| BstV1I | GCAGC | 8 cut(s) | 245, 335, 358, 361, 465, 471, 506, 509 |
| BstV2I | GAAGAC | 2 cut(s) | 180, 800 |
| Bsu15I | ATCGAT | 2 cut(s) | 360, 434 |
| BsuRI | GGCC | 6 cut(s) | 18, 198, 250, 295, 309, 467 |
| BsuTUI | ATCGAT | 2 cut(s) | 360, 434 |
| BtgI | CCRYGG | 1 cut(s) | 241 |
| BtsCI | GGATG | 2 cut(s) | 285, 820 |
| BtsI | GCAGTG | 1 cut(s) | 675 |
| BtsIMutI | CAGTG | 3 cut(s) | 136, 675, 881 |
| Cac8I | GCNNGC | 5 cut(s) | 115, 216, 346, 385, 893 |
| CaiI | CAGNNNCTG | 1 cut(s) | 475 |
| CciI | TCATGA | 1 cut(s) | 844 |
| CfoI | GCGC | 6 cut(s) | 182, 304, 346, 348, 405, 415 |
| Cfr13I | GGNCC | 3 cut(s) | 197, 294, 466 |
| Cfr42I | CCGCGG | 1 cut(s) | 244 |
| ClaI | ATCGAT | 2 cut(s) | 360, 434 |
| CseI | GACGC | 2 cut(s) | 290, 500 |
| Csp6I | GTAC | 2 cut(s) | 136, 625 |
| CviAII | CATG | 1 cut(s) | 845 |
| CviQI | GTAC | 2 cut(s) | 136, 625 |
| DdeI | CTNAG | 4 cut(s) | 41, 340, 610, 746 |
| DpnI | GATC | 6 cut(s) | 269, 363, 433, 437, 579, 762 |
| DpnII | GATC | 6 cut(s) | 267, 361, 431, 435, 577, 760 |
| EaeI | YGGCCR | 1 cut(s) | 16 |
| Eam1104I | CTCTTC | 2 cut(s) | 397, 549 |
| EarI | CTCTTC | 2 cut(s) | 397, 549 |
| EciI | GGCGGA | 2 cut(s) | 68, 428 |
| EcoRII | CCWGG | 1 cut(s) | 224 |
| FaeI | CATG | 1 cut(s) | 848 |
| FaiI | YATR | 4 cut(s) | 257, 316, 756, 846 |
| FaqI | GGGAC | 1 cut(s) | 840 |
| FatI | CATG | 1 cut(s) | 844 |
| FauI | CCCGC | 2 cut(s) | 106, 408 |
| FokI | GGATG | 2 cut(s) | 272, 827 |
| FspBI | CTAG | 3 cut(s) | 410, 429, 522 |
| GlaI | GCGC | 6 cut(s) | 181, 303, 345, 347, 404, 414 |
| GsaI | CCCAGC | 3 cut(s) | 214, 472, 778 |
| HaeII | RGCGCY | 2 cut(s) | 183, 416 |
| HaeIII | GGCC | 6 cut(s) | 18, 198, 250, 295, 309, 467 |
| HapII | CCGG | 5 cut(s) | 195, 278, 417, 442, 454 |
| HgaI | GACGC | 2 cut(s) | 290, 500 |
| HhaI | GCGC | 6 cut(s) | 182, 304, 346, 348, 405, 415 |
| Hin1I | GRCGYC | 1 cut(s) | 282 |
| Hin1II | CATG | 1 cut(s) | 848 |
| Hin6I | GCGC | 6 cut(s) | 180, 302, 344, 346, 403, 413 |
| HinP1I | GCGC | 6 cut(s) | 180, 302, 344, 346, 403, 413 |
| HinfI | GANTC | 3 cut(s) | 84, 152, 608 |
| HpaII | CCGG | 5 cut(s) | 195, 278, 417, 442, 454 |
| HphI | GGTGA | 2 cut(s) | 195, 733 |
| Hpy166II | GTNNAC | 1 cut(s) | 136 |
| Hpy188I | TCNGA | 4 cut(s) | 13, 37, 613, 747 |
| Hpy188III | TCNNGA | 4 cut(s) | 63, 265, 429, 845 |
| Hpy8I | GTNNAC | 1 cut(s) | 136 |
| Hpy99I | CGWCG | 2 cut(s) | 371, 494 |
| HpyAV | CCTTC | 4 cut(s) | 271, 406, 527, 608 |
| HpyCH4III | ACNGT | 4 cut(s) | 140, 632, 810, 871 |
| HpyCH4IV | ACGT | 1 cut(s) | 829 |
| HpyCH4V | TGCA | 5 cut(s) | 81, 123, 314, 328, 481 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 37, 101, 110, 215, 301, 345, 380, 459, 478 |
| HpyF3I | CTNAG | 4 cut(s) | 41, 340, 610, 746 |
| HpySE526I | ACGT | 1 cut(s) | 829 |
| Hsp92I | GRCGYC | 1 cut(s) | 282 |
| Hsp92II | CATG | 1 cut(s) | 848 |
| HspAI | GCGC | 6 cut(s) | 180, 302, 344, 346, 403, 413 |
| KspI | CCGCGG | 1 cut(s) | 244 |
| Kzo9I | GATC | 6 cut(s) | 267, 361, 431, 435, 577, 760 |
| LguI | GCTCTTC | 1 cut(s) | 397 |
| LmnI | GCTCC | 6 cut(s) | 37, 55, 197, 277, 456, 861 |
| Lsp1109I | GCAGC | 8 cut(s) | 245, 335, 358, 361, 465, 471, 506, 509 |
| LweI | GCATC | 1 cut(s) | 805 |
| MaeI | CTAG | 3 cut(s) | 410, 429, 522 |
| MaeII | ACGT | 1 cut(s) | 829 |
| MalI | GATC | 6 cut(s) | 269, 363, 433, 437, 579, 762 |
| MboI | GATC | 6 cut(s) | 267, 361, 431, 435, 577, 760 |
| MboII | GAAGA | 7 cut(s) | 85, 178, 185, 384, 563, 566, 805 |
| MluCI | AATT | 3 cut(s) | 570, 593, 863 |
| MlyI | GAGTC | 2 cut(s) | 146, 602 |
| MmeI | TCCRAC | 3 cut(s) | 15, 313, 605 |
| MroXI | GAANNNNTTC | 1 cut(s) | 783 |
| MseI | TTAA | 2 cut(s) | 737, 822 |
| MspA1I | CMGCKG | 3 cut(s) | 243, 387, 774 |
| MspCI | CTTAAG | 1 cut(s) | 821 |
| MspI | CCGG | 5 cut(s) | 195, 278, 417, 442, 454 |
| MspR9I | CCNGG | 2 cut(s) | 226, 417 |
| MvaI | CCWGG | 1 cut(s) | 226 |
| MvnI | CGCG | 3 cut(s) | 243, 346, 403 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 37, 101, 110, 215, 301, 345, 380, 459, 478 |
| NciI | CCSGG | 1 cut(s) | 417 |
| NdeII | GATC | 6 cut(s) | 267, 361, 431, 435, 577, 760 |
| NlaIII | CATG | 1 cut(s) | 848 |
| NlaIV | GGNNCC | 4 cut(s) | 10, 57, 193, 199 |
| NmeAIII | GCCGAG | 1 cut(s) | 44 |
| PagI | TCATGA | 1 cut(s) | 844 |
| PauI | GCGCGC | 1 cut(s) | 344 |
| PciSI | GCTCTTC | 1 cut(s) | 397 |
| PdmI | GAANNNNTTC | 1 cut(s) | 783 |
| PfeI | GAWTC | 1 cut(s) | 84 |
| PleI | GAGTC | 2 cut(s) | 146, 602 |
| PpsI | GAGTC | 2 cut(s) | 146, 602 |
| Psp6I | CCWGG | 1 cut(s) | 224 |
| PspFI | CCCAGC | 3 cut(s) | 210, 468, 774 |
| PspGI | CCWGG | 1 cut(s) | 224 |
| PspN4I | GGNNCC | 4 cut(s) | 10, 57, 193, 199 |
| PspPI | GGNCC | 3 cut(s) | 197, 294, 466 |
| PstNI | CAGNNNCTG | 1 cut(s) | 475 |
| PteI | GCGCGC | 1 cut(s) | 344 |
| RsaI | GTAC | 2 cut(s) | 137, 626 |
| RsaNI | GTAC | 2 cut(s) | 136, 625 |
| SacII | CCGCGG | 1 cut(s) | 244 |
| SapI | GCTCTTC | 1 cut(s) | 397 |
| SaqAI | TTAA | 2 cut(s) | 737, 822 |
| Sau3AI | GATC | 6 cut(s) | 267, 361, 431, 435, 577, 760 |
| Sau96I | GGNCC | 3 cut(s) | 197, 294, 466 |
| SchI | GAGTC | 2 cut(s) | 146, 602 |
| ScrFI | CCNGG | 2 cut(s) | 226, 417 |
| SfaNI | GCATC | 1 cut(s) | 805 |
| Sfr303I | CCGCGG | 1 cut(s) | 244 |
| SgrBI | CCGCGG | 1 cut(s) | 244 |
| SmlI | CTYRAG | 2 cut(s) | 620, 821 |
| SmoI | CTYRAG | 2 cut(s) | 620, 821 |
| Sse9I | AATT | 3 cut(s) | 570, 593, 863 |
| SspMI | CTAG | 3 cut(s) | 410, 429, 522 |
| StyD4I | CCNGG | 2 cut(s) | 224, 415 |
| TaaI | ACNGT | 4 cut(s) | 140, 632, 810, 871 |
| TaiI | ACGT | 1 cut(s) | 832 |
| TaqI | TCGA | 5 cut(s) | 150, 354, 360, 434, 586 |
| TasI | AATT | 3 cut(s) | 570, 593, 863 |
| TatI | WGTACW | 1 cut(s) | 135 |
| TauI | GCSGC | 2 cut(s) | 31, 390 |
| TfiI | GAWTC | 1 cut(s) | 84 |
| Tru1I | TTAA | 2 cut(s) | 737, 822 |
| Tru9I | TTAA | 2 cut(s) | 737, 822 |
| TscAI | CASTG | 3 cut(s) | 136, 682, 881 |
| TseI | GCWGC | 8 cut(s) | 233, 348, 371, 374, 459, 478, 494, 497 |
| TspDTI | ATGAA | 2 cut(s) | 720, 897 |
| TspRI | CASTG | 3 cut(s) | 136, 682, 881 |
| Vha464I | CTTAAG | 1 cut(s) | 821 |
| XapI | RAATTY | 1 cut(s) | 593 |
| XbaI | TCTAGA | 1 cut(s) | 428 |
| XmnI | GAANNNNTTC | 1 cut(s) | 783 |
| XspI | CTAG | 3 cut(s) | 410, 429, 522 |