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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 441 |
| AarI | CACCTGC | 1 cut(s) | 299 |
| Acc36I | ACCTGC | 1 cut(s) | 299 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 600 |
| AccII | CGCG | 5 cut(s) | 178, 212, 852, 862, 867 |
| AciI | CCGC | 9 cut(s) | 152, 176, 212, 790, 799, 806, 830, 855, 865 |
| AclWI | GGATC | 5 cut(s) | 12, 289, 313, 479, 709 |
| AcoI | YGGCCR | 1 cut(s) | 69 |
| AcsI | RAATTY | 1 cut(s) | 679 |
| AcuI | CTGAAG | 2 cut(s) | 494, 729 |
| AcvI | CACGTG | 1 cut(s) | 332 |
| AcyI | GRCGYC | 1 cut(s) | 521 |
| AfaI | GTAC | 3 cut(s) | 66, 347, 506 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 211, 355, 499, 600, 805 |
| AflII | CTTAAG | 1 cut(s) | 545 |
| AflIII | ACRYGT | 1 cut(s) | 654 |
| AgsI | TTSAA | 2 cut(s) | 517, 646 |
| AjiI | CACGTC | 1 cut(s) | 674 |
| AjnI | CCWGG | 1 cut(s) | 801 |
| AluBI | AGCT | 2 cut(s) | 246, 887 |
| AluI | AGCT | 2 cut(s) | 246, 887 |
| Alw26I | GTCTC | 1 cut(s) | 729 |
| AlwI | GGATC | 5 cut(s) | 12, 289, 313, 479, 709 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 249 |
| AoxI | GGCC | 1 cut(s) | 69 |
| ApeKI | GCWGC | 2 cut(s) | 294, 326 |
| ApoI | RAATTY | 1 cut(s) | 679 |
| AspLEI | GCGC | 5 cut(s) | 267, 663, 854, 864, 869 |
| AspS9I | GGNCC | 1 cut(s) | 827 |
| AsuHPI | GGTGA | 3 cut(s) | 108, 280, 457 |
| AvaII | GGWCC | 1 cut(s) | 827 |
| BaeGI | GKGCMC | 1 cut(s) | 152 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 329, 362 |
| BanII | GRGCYC | 2 cut(s) | 98, 315 |
| BbrPI | CACGTG | 1 cut(s) | 332 |
| BbsI | GAAGAC | 1 cut(s) | 524 |
| BbvCI | CCTCAGC | 1 cut(s) | 197 |
| BbvI | GCAGC | 2 cut(s) | 306, 338 |
| BceAI | ACGGC | 1 cut(s) | 56 |
| BciT130I | CCWGG | 1 cut(s) | 803 |
| BciVI | GTATCC | 1 cut(s) | 532 |
| BcoDI | GTCTC | 1 cut(s) | 729 |
| BfaI | CTAG | 4 cut(s) | 257, 489, 758, 776 |
| BfmI | CTRYAG | 2 cut(s) | 292, 811 |
| BfoI | RGCGCY | 1 cut(s) | 268 |
| BfrI | CTTAAG | 1 cut(s) | 545 |
| BfuAI | ACCTGC | 1 cut(s) | 299 |
| BfuI | GTATCC | 1 cut(s) | 532 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 807 |
| BisI | GCNGC | 5 cut(s) | 213, 295, 327, 855, 865 |
| BlsI | GCNGC | 5 cut(s) | 214, 296, 328, 856, 866 |
| Bme1390I | CCNGG | 1 cut(s) | 803 |
| Bme18I | GGWCC | 1 cut(s) | 827 |
| BmgBI | CACGTC | 1 cut(s) | 674 |
| BmgT120I | GGNCC | 1 cut(s) | 827 |
| BmrFI | CCNGG | 1 cut(s) | 803 |
| BmrI | ACTGGG | 4 cut(s) | 94, 269, 293, 906 |
| BmsI | GCATC | 1 cut(s) | 887 |
| BmuI | ACTGGG | 4 cut(s) | 94, 269, 293, 906 |
| BpiI | GAAGAC | 1 cut(s) | 524 |
| BpmI | CTGGAG | 1 cut(s) | 819 |
| Bpu10I | CCTNAGC | 1 cut(s) | 197 |
| BpuEI | CTTGAG | 1 cut(s) | 362 |
| Bsa29I | ATCGAT | 1 cut(s) | 284 |
| BsaAI | YACGTR | 1 cut(s) | 332 |
| BsaHI | GRCGYC | 1 cut(s) | 521 |
| BsaJI | CCNNGG | 1 cut(s) | 817 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 207, 237 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 211, 355, 499, 600, 805 |
| Bse1I | ACTGG | 7 cut(s) | 28, 72, 100, 275, 299, 766, 901 |
| BseBI | CCWGG | 1 cut(s) | 803 |
| BseCI | ATCGAT | 1 cut(s) | 284 |
| BseDI | CCNNGG | 1 cut(s) | 817 |
| BseGI | GGATG | 3 cut(s) | 55, 104, 123 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 211, 355, 499, 600, 805 |
| BseMII | CTCAG | 2 cut(s) | 211, 461 |
| BseNI | ACTGG | 7 cut(s) | 28, 72, 100, 275, 299, 766, 901 |
| BseRI | GAGGAG | 1 cut(s) | 185 |
| BseSI | GKGCMC | 1 cut(s) | 152 |
| BseXI | GCAGC | 2 cut(s) | 306, 338 |
| BseYI | CCCAGC | 3 cut(s) | 162, 242, 498 |
| BsgI | GTGCAG | 3 cut(s) | 48, 126, 235 |
| Bsh1236I | CGCG | 5 cut(s) | 178, 212, 852, 862, 867 |
| BshFI | GGCC | 1 cut(s) | 71 |
| BshVI | ATCGAT | 1 cut(s) | 284 |
| BslFI | GGGAC | 2 cut(s) | 165, 564 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 211, 355, 499, 600, 805 |
| BsmAI | GTCTC | 1 cut(s) | 729 |
| BsmFI | GGGAC | 2 cut(s) | 165, 564 |
| BsnI | GGCC | 1 cut(s) | 71 |
| Bsp1286I | GDGCHC | 3 cut(s) | 98, 152, 315 |
| Bsp143I | GATC | 7 cut(s) | 4, 281, 285, 305, 484, 714, 838 |
| BspACI | CCGC | 9 cut(s) | 152, 176, 212, 790, 799, 806, 830, 855, 865 |
| BspANI | GGCC | 1 cut(s) | 71 |
| BspCNI | CTCAG | 2 cut(s) | 210, 462 |
| BspDI | ATCGAT | 1 cut(s) | 284 |
| BspFNI | CGCG | 5 cut(s) | 178, 212, 852, 862, 867 |
| BspHI | TCATGA | 1 cut(s) | 568 |
| BspMAI | CTGCAG | 1 cut(s) | 296 |
| BspMI | ACCTGC | 1 cut(s) | 299 |
| BspPI | GGATC | 5 cut(s) | 12, 289, 313, 479, 709 |
| BspTI | CTTAAG | 1 cut(s) | 545 |
| BsrI | ACTGG | 7 cut(s) | 28, 72, 100, 275, 299, 766, 901 |
| BssECI | CCNNGG | 1 cut(s) | 817 |
| BssMI | GATC | 7 cut(s) | 4, 281, 285, 305, 484, 714, 838 |
| BssNI | GRCGYC | 1 cut(s) | 521 |
| Bst2UI | CCWGG | 1 cut(s) | 803 |
| Bst4CI | ACNGT | 5 cut(s) | 147, 322, 353, 432, 534 |
| BstACI | GRCGYC | 1 cut(s) | 521 |
| BstAFI | CTTAAG | 1 cut(s) | 545 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 140 |
| BstBAI | YACGTR | 1 cut(s) | 332 |
| BstC8I | GCNNGC | 4 cut(s) | 141, 152, 210, 808 |
| BstDEI | CTNAG | 2 cut(s) | 197, 470 |
| BstF5I | GGATG | 3 cut(s) | 55, 104, 123 |
| BstFNI | CGCG | 5 cut(s) | 178, 212, 852, 862, 867 |
| BstH2I | RGCGCY | 1 cut(s) | 268 |
| BstHHI | GCGC | 5 cut(s) | 267, 663, 854, 864, 869 |
| BstKTI | GATC | 7 cut(s) | 7, 284, 288, 308, 487, 717, 841 |
| BstMAI | GTCTC | 1 cut(s) | 729 |
| BstMBI | GATC | 7 cut(s) | 4, 281, 285, 305, 484, 714, 838 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 93, 140, 383, 807, 849, 875, 884 |
| BstNI | CCWGG | 1 cut(s) | 803 |
| BstNSI | RCATGY | 2 cut(s) | 658, 790 |
| BstSCI | CCNGG | 1 cut(s) | 801 |
| BstSFI | CTRYAG | 2 cut(s) | 292, 811 |
| BstSLI | GKGCMC | 1 cut(s) | 152 |
| BstUI | CGCG | 5 cut(s) | 178, 212, 852, 862, 867 |
| BstV1I | GCAGC | 2 cut(s) | 306, 338 |
| BstV2I | GAAGAC | 1 cut(s) | 524 |
| BstX2I | RGATCY | 1 cut(s) | 305 |
| BstYI | RGATCY | 1 cut(s) | 305 |
| Bsu15I | ATCGAT | 1 cut(s) | 284 |
| BsuI | GTATCC | 1 cut(s) | 532 |
| BsuRI | GGCC | 1 cut(s) | 71 |
| BsuTUI | ATCGAT | 1 cut(s) | 284 |
| BtrI | CACGTC | 1 cut(s) | 674 |
| BtsCI | GGATG | 3 cut(s) | 55, 104, 123 |
| BtsI | GCAGTG | 4 cut(s) | 36, 134, 168, 396 |
| BtsIMutI | CAGTG | 6 cut(s) | 36, 134, 152, 168, 396, 605 |
| BveI | ACCTGC | 1 cut(s) | 299 |
| Cac8I | GCNNGC | 4 cut(s) | 141, 152, 210, 808 |
| CaiI | CAGNNNCTG | 1 cut(s) | 249 |
| CciI | TCATGA | 1 cut(s) | 568 |
| CfoI | GCGC | 5 cut(s) | 267, 663, 854, 864, 869 |
| Cfr13I | GGNCC | 1 cut(s) | 827 |
| ClaI | ATCGAT | 1 cut(s) | 284 |
| CseI | GACGC | 3 cut(s) | 167, 529, 858 |
| Csp6I | GTAC | 3 cut(s) | 65, 346, 505 |
| CviAII | CATG | 3 cut(s) | 569, 655, 787 |
| CviQI | GTAC | 3 cut(s) | 65, 346, 505 |
| DdeI | CTNAG | 2 cut(s) | 197, 470 |
| DpnI | GATC | 7 cut(s) | 6, 283, 287, 307, 486, 716, 840 |
| DpnII | GATC | 7 cut(s) | 4, 281, 285, 305, 484, 714, 838 |
| EaeI | YGGCCR | 1 cut(s) | 69 |
| Eco24I | GRGCYC | 2 cut(s) | 98, 315 |
| Eco47I | GGWCC | 1 cut(s) | 827 |
| Eco57I | CTGAAG | 2 cut(s) | 494, 729 |
| Eco72I | CACGTG | 1 cut(s) | 332 |
| EcoRII | CCWGG | 1 cut(s) | 801 |
| EcoT38I | GRGCYC | 2 cut(s) | 98, 315 |
| FaeI | CATG | 3 cut(s) | 572, 658, 790 |
| FaqI | GGGAC | 2 cut(s) | 165, 564 |
| FatI | CATG | 3 cut(s) | 568, 654, 786 |
| FauI | CCCGC | 2 cut(s) | 159, 799 |
| Fnu4HI | GCNGC | 5 cut(s) | 213, 295, 327, 855, 865 |
| FokI | GGATG | 3 cut(s) | 62, 91, 110 |
| FriOI | GRGCYC | 2 cut(s) | 98, 315 |
| Fsp4HI | GCNGC | 5 cut(s) | 213, 295, 327, 855, 865 |
| FspBI | CTAG | 4 cut(s) | 257, 489, 758, 776 |
| GlaI | GCGC | 5 cut(s) | 266, 662, 853, 863, 868 |
| GluI | GCNGC | 5 cut(s) | 213, 295, 327, 855, 865 |
| GsaI | CCCAGC | 3 cut(s) | 166, 246, 502 |
| GsuI | CTGGAG | 1 cut(s) | 819 |
| HaeII | RGCGCY | 1 cut(s) | 268 |
| HaeIII | GGCC | 1 cut(s) | 71 |
| HgaI | GACGC | 3 cut(s) | 167, 529, 858 |
| HhaI | GCGC | 5 cut(s) | 267, 663, 854, 864, 869 |
| Hin1I | GRCGYC | 1 cut(s) | 521 |
| Hin1II | CATG | 3 cut(s) | 572, 658, 790 |
| Hin6I | GCGC | 5 cut(s) | 265, 661, 852, 862, 867 |
| HinP1I | GCGC | 5 cut(s) | 265, 661, 852, 862, 867 |
| HindIII | AAGCTT | 1 cut(s) | 885 |
| HinfI | GANTC | 1 cut(s) | 633 |
| HphI | GGTGA | 3 cut(s) | 108, 280, 457 |
| Hpy166II | GTNNAC | 1 cut(s) | 65 |
| Hpy188I | TCNGA | 4 cut(s) | 364, 471, 883, 892 |
| Hpy188III | TCNNGA | 3 cut(s) | 569, 718, 836 |
| Hpy8I | GTNNAC | 1 cut(s) | 65 |
| Hpy99I | CGWCG | 2 cut(s) | 326, 852 |
| HpyAV | CCTTC | 2 cut(s) | 329, 518 |
| HpyCH4III | ACNGT | 5 cut(s) | 147, 322, 353, 432, 534 |
| HpyCH4IV | ACGT | 3 cut(s) | 331, 553, 673 |
| HpyCH4V | TGCA | 5 cut(s) | 29, 57, 143, 252, 294 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 93, 140, 383, 807, 849, 875, 884 |
| HpyF3I | CTNAG | 2 cut(s) | 197, 470 |
| HpySE526I | ACGT | 3 cut(s) | 331, 553, 673 |
| Hsp92I | GRCGYC | 1 cut(s) | 521 |
| Hsp92II | CATG | 3 cut(s) | 572, 658, 790 |
| HspAI | GCGC | 5 cut(s) | 265, 661, 852, 862, 867 |
| Kzo9I | GATC | 7 cut(s) | 4, 281, 285, 305, 484, 714, 838 |
| LmnI | GCTCC | 4 cut(s) | 178, 198, 251, 585 |
| Lsp1109I | GCAGC | 2 cut(s) | 306, 338 |
| LweI | GCATC | 1 cut(s) | 887 |
| MaeI | CTAG | 4 cut(s) | 257, 489, 758, 776 |
| MaeII | ACGT | 3 cut(s) | 331, 553, 673 |
| MalI | GATC | 7 cut(s) | 6, 283, 287, 307, 486, 716, 840 |
| MboI | GATC | 7 cut(s) | 4, 281, 285, 305, 484, 714, 838 |
| MboII | GAAGA | 2 cut(s) | 529, 716 |
| MflI | RGATCY | 1 cut(s) | 305 |
| MhlI | GDGCHC | 3 cut(s) | 98, 152, 315 |
| MluCI | AATT | 3 cut(s) | 44, 436, 679 |
| MlyI | GAGTC | 1 cut(s) | 642 |
| MmeI | TCCRAC | 2 cut(s) | 283, 805 |
| MnlI | CCTC | 7 cut(s) | 124, 138, 206, 233, 465, 827, 843 |
| MseI | TTAA | 2 cut(s) | 461, 546 |
| MspCI | CTTAAG | 1 cut(s) | 545 |
| MspR9I | CCNGG | 1 cut(s) | 803 |
| MteI | GCGCNGCGC | 1 cut(s) | 865 |
| MvaI | CCWGG | 1 cut(s) | 803 |
| MvnI | CGCG | 5 cut(s) | 178, 212, 852, 862, 867 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 93, 140, 383, 807, 849, 875, 884 |
| NdeII | GATC | 7 cut(s) | 4, 281, 285, 305, 484, 714, 838 |
| NlaIII | CATG | 3 cut(s) | 572, 658, 790 |
| NmeAIII | GCCGAG | 1 cut(s) | 798 |
| NspI | RCATGY | 2 cut(s) | 658, 790 |
| PagI | TCATGA | 1 cut(s) | 568 |
| PaqCI | CACCTGC | 1 cut(s) | 299 |
| PciI | ACATGT | 1 cut(s) | 654 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 600 |
| PkrI | GCNGC | 5 cut(s) | 214, 296, 328, 856, 866 |
| PleI | GAGTC | 1 cut(s) | 641 |
| PmaCI | CACGTG | 1 cut(s) | 332 |
| PmlI | CACGTG | 1 cut(s) | 332 |
| PpsI | GAGTC | 1 cut(s) | 641 |
| Ppu21I | YACGTR | 1 cut(s) | 332 |
| PscI | ACATGT | 1 cut(s) | 654 |
| PsiI | TTATAA | 1 cut(s) | 441 |
| Psp6I | CCWGG | 1 cut(s) | 801 |
| PspCI | CACGTG | 1 cut(s) | 332 |
| PspFI | CCCAGC | 3 cut(s) | 162, 242, 498 |
| PspGI | CCWGG | 1 cut(s) | 801 |
| PspPI | GGNCC | 1 cut(s) | 827 |
| PstI | CTGCAG | 1 cut(s) | 296 |
| PstNI | CAGNNNCTG | 1 cut(s) | 249 |
| PsuI | RGATCY | 1 cut(s) | 305 |
| RsaI | GTAC | 3 cut(s) | 66, 347, 506 |
| RsaNI | GTAC | 3 cut(s) | 65, 346, 505 |
| SaqAI | TTAA | 2 cut(s) | 461, 546 |
| SatI | GCNGC | 5 cut(s) | 213, 295, 327, 855, 865 |
| Sau3AI | GATC | 7 cut(s) | 4, 281, 285, 305, 484, 714, 838 |
| Sau96I | GGNCC | 1 cut(s) | 827 |
| SchI | GAGTC | 1 cut(s) | 642 |
| ScrFI | CCNGG | 1 cut(s) | 803 |
| SduI | GDGCHC | 3 cut(s) | 98, 152, 315 |
| SfaNI | GCATC | 1 cut(s) | 887 |
| SfcI | CTRYAG | 2 cut(s) | 292, 811 |
| SinI | GGWCC | 1 cut(s) | 827 |
| SmlI | CTYRAG | 2 cut(s) | 341, 545 |
| SmoI | CTYRAG | 2 cut(s) | 341, 545 |
| Sse9I | AATT | 3 cut(s) | 44, 436, 679 |
| SsiI | CCGC | 9 cut(s) | 152, 176, 212, 790, 799, 806, 830, 855, 865 |
| SspMI | CTAG | 4 cut(s) | 257, 489, 758, 776 |
| StyD4I | CCNGG | 1 cut(s) | 801 |
| TaaI | ACNGT | 5 cut(s) | 147, 322, 353, 432, 534 |
| TaiI | ACGT | 3 cut(s) | 334, 556, 676 |
| TaqI | TCGA | 1 cut(s) | 284 |
| TasI | AATT | 3 cut(s) | 44, 436, 679 |
| TatI | WGTACW | 1 cut(s) | 64 |
| TauI | GCSGC | 3 cut(s) | 215, 857, 867 |
| Tru1I | TTAA | 2 cut(s) | 461, 546 |
| Tru9I | TTAA | 2 cut(s) | 461, 546 |
| TscAI | CASTG | 6 cut(s) | 36, 141, 152, 175, 403, 605 |
| TseI | GCWGC | 2 cut(s) | 294, 326 |
| TspDTI | ATGAA | 4 cut(s) | 57, 441, 557, 621 |
| TspRI | CASTG | 6 cut(s) | 36, 141, 152, 175, 403, 605 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 600 |
| Vha464I | CTTAAG | 1 cut(s) | 545 |
| VpaK11BI | GGWCC | 1 cut(s) | 827 |
| XapI | RAATTY | 1 cut(s) | 679 |
| XceI | RCATGY | 2 cut(s) | 658, 790 |
| XspI | CTAG | 4 cut(s) | 257, 489, 758, 776 |