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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 921 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 64 |
| AciI | CCGC | 2 cut(s) | 319, 913 |
| AclWI | GGATC | 7 cut(s) | 2, 116, 185, 662, 723, 851, 937 |
| AcsI | RAATTY | 1 cut(s) | 597 |
| AcuI | CTGAAG | 2 cut(s) | 200, 1028 |
| AcvI | CACGTG | 2 cut(s) | 680, 698 |
| AdeI | CACNNNGTG | 1 cut(s) | 698 |
| AfaI | GTAC | 4 cut(s) | 56, 212, 827, 1013 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 64, 205, 295, 648 |
| AflIII | ACRYGT | 1 cut(s) | 360 |
| AgsI | TTSAA | 5 cut(s) | 223, 352, 565, 851, 956 |
| AjiI | CACGTC | 2 cut(s) | 380, 662 |
| AjnI | CCWGG | 2 cut(s) | 289, 575 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 285, 317 |
| AluBI | AGCT | 2 cut(s) | 455, 539 |
| AluI | AGCT | 2 cut(s) | 455, 539 |
| Alw26I | GTCTC | 2 cut(s) | 386, 538 |
| AlwI | GGATC | 7 cut(s) | 2, 116, 185, 662, 723, 851, 937 |
| Ama87I | CYCGRG | 1 cut(s) | 602 |
| AoxI | GGCC | 1 cut(s) | 578 |
| ApeKI | GCWGC | 1 cut(s) | 779 |
| ApoI | RAATTY | 1 cut(s) | 597 |
| AspLEI | GCGC | 1 cut(s) | 369 |
| AspS9I | GGNCC | 1 cut(s) | 579 |
| AsuHPI | GGTGA | 2 cut(s) | 163, 856 |
| AsuII | TTCGAA | 1 cut(s) | 983 |
| AvaI | CYCGRG | 1 cut(s) | 602 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 38, 71 |
| BanI | GGYRCC | 1 cut(s) | 921 |
| BbrPI | CACGTG | 2 cut(s) | 680, 698 |
| BbsI | GAAGAC | 1 cut(s) | 230 |
| BbvI | GCAGC | 1 cut(s) | 791 |
| BccI | CCATC | 1 cut(s) | 66 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 911, 945 |
| BciT130I | CCWGG | 2 cut(s) | 291, 577 |
| BcoDI | GTCTC | 2 cut(s) | 386, 538 |
| BfaI | CTAG | 2 cut(s) | 195, 464 |
| BfmI | CTRYAG | 1 cut(s) | 763 |
| BisI | GCNGC | 1 cut(s) | 780 |
| BlsI | GCNGC | 1 cut(s) | 781 |
| Bme1390I | CCNGG | 2 cut(s) | 291, 577 |
| BmeT110I | CYCGRG | 1 cut(s) | 602 |
| BmgBI | CACGTC | 2 cut(s) | 380, 662 |
| BmgT120I | GGNCC | 1 cut(s) | 579 |
| BmiI | GGNNCC | 1 cut(s) | 923 |
| BmrFI | CCNGG | 2 cut(s) | 291, 577 |
| BmrI | ACTGGG | 1 cut(s) | 594 |
| BmsI | GCATC | 3 cut(s) | 151, 235, 720 |
| BmuI | ACTGGG | 1 cut(s) | 594 |
| BpiI | GAAGAC | 1 cut(s) | 230 |
| Bpu14I | TTCGAA | 1 cut(s) | 983 |
| BpuEI | CTTGAG | 1 cut(s) | 71 |
| BsaAI | YACGTR | 2 cut(s) | 680, 698 |
| BsaBI | GATNNNNATC | 1 cut(s) | 842 |
| BsaJI | CCNNGG | 3 cut(s) | 289, 603, 925 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 64, 205, 295, 648 |
| Bse1I | ACTGG | 3 cut(s) | 300, 472, 589 |
| Bse8I | GATNNNNATC | 1 cut(s) | 842 |
| BseBI | CCWGG | 2 cut(s) | 291, 577 |
| BseDI | CCNNGG | 3 cut(s) | 289, 603, 925 |
| BseGI | GGATG | 2 cut(s) | 250, 971 |
| BseJI | GATNNNNATC | 1 cut(s) | 842 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 64, 205, 295, 648 |
| BseMII | CTCAG | 3 cut(s) | 54, 167, 531 |
| BseNI | ACTGG | 3 cut(s) | 300, 472, 589 |
| BseXI | GCAGC | 1 cut(s) | 791 |
| BseYI | CCCAGC | 1 cut(s) | 204 |
| BshFI | GGCC | 1 cut(s) | 580 |
| BshNI | GGYRCC | 1 cut(s) | 921 |
| BsiHKCI | CYCGRG | 1 cut(s) | 602 |
| BslFI | GGGAC | 3 cut(s) | 601, 720, 834 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 64, 205, 295, 648 |
| BsmAI | GTCTC | 2 cut(s) | 386, 538 |
| BsmBI | CGTCTC | 1 cut(s) | 386 |
| BsmFI | GGGAC | 3 cut(s) | 601, 720, 834 |
| BsnI | GGCC | 1 cut(s) | 580 |
| BsoBI | CYCGRG | 1 cut(s) | 602 |
| Bsp119I | TTCGAA | 1 cut(s) | 983 |
| Bsp143I | GATC | 8 cut(s) | 7, 121, 190, 420, 667, 715, 843, 942 |
| BspACI | CCGC | 2 cut(s) | 319, 913 |
| BspANI | GGCC | 1 cut(s) | 580 |
| BspCNI | CTCAG | 3 cut(s) | 53, 168, 532 |
| BspLI | GGNNCC | 1 cut(s) | 923 |
| BspPI | GGATC | 7 cut(s) | 2, 116, 185, 662, 723, 851, 937 |
| BspT104I | TTCGAA | 1 cut(s) | 983 |
| BspT107I | GGYRCC | 1 cut(s) | 921 |
| BsrI | ACTGG | 3 cut(s) | 300, 472, 589 |
| BssECI | CCNNGG | 3 cut(s) | 289, 603, 925 |
| BssMI | GATC | 8 cut(s) | 7, 121, 190, 420, 667, 715, 843, 942 |
| Bst2UI | CCWGG | 2 cut(s) | 291, 577 |
| Bst4CI | ACNGT | 3 cut(s) | 240, 825, 862 |
| BstBAI | YACGTR | 2 cut(s) | 680, 698 |
| BstBI | TTCGAA | 1 cut(s) | 983 |
| BstC8I | GCNNGC | 2 cut(s) | 323, 530 |
| BstDEI | CTNAG | 5 cut(s) | 21, 40, 176, 540, 805 |
| BstF5I | GGATG | 2 cut(s) | 250, 971 |
| BstHHI | GCGC | 1 cut(s) | 369 |
| BstKTI | GATC | 8 cut(s) | 10, 124, 193, 423, 670, 718, 846, 945 |
| BstMAI | GTCTC | 2 cut(s) | 386, 538 |
| BstMBI | GATC | 8 cut(s) | 7, 121, 190, 420, 667, 715, 843, 942 |
| BstNI | CCWGG | 2 cut(s) | 291, 577 |
| BstNSI | RCATGY | 1 cut(s) | 364 |
| BstSCI | CCNGG | 2 cut(s) | 289, 575 |
| BstSFI | CTRYAG | 1 cut(s) | 763 |
| BstV1I | GCAGC | 1 cut(s) | 791 |
| BstV2I | GAAGAC | 1 cut(s) | 230 |
| BsuRI | GGCC | 1 cut(s) | 580 |
| BtrI | CACGTC | 2 cut(s) | 380, 662 |
| BtsCI | GGATG | 2 cut(s) | 250, 971 |
| BtsI | GCAGTG | 1 cut(s) | 105 |
| BtsIMutI | CAGTG | 5 cut(s) | 105, 236, 311, 582, 885 |
| Cac8I | GCNNGC | 2 cut(s) | 323, 530 |
| CfoI | GCGC | 1 cut(s) | 369 |
| Cfr13I | GGNCC | 1 cut(s) | 579 |
| Csp6I | GTAC | 4 cut(s) | 55, 211, 826, 1012 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 373, 408 |
| CviAII | CATG | 5 cut(s) | 361, 710, 719, 908, 937 |
| CviQI | GTAC | 4 cut(s) | 55, 211, 826, 1012 |
| DdeI | CTNAG | 5 cut(s) | 21, 40, 176, 540, 805 |
| DpnI | GATC | 8 cut(s) | 9, 123, 192, 422, 669, 717, 845, 944 |
| DpnII | GATC | 8 cut(s) | 7, 121, 190, 420, 667, 715, 843, 942 |
| DraIII | CACNNNGTG | 1 cut(s) | 698 |
| Eco57I | CTGAAG | 2 cut(s) | 200, 1028 |
| Eco72I | CACGTG | 2 cut(s) | 680, 698 |
| Eco88I | CYCGRG | 1 cut(s) | 602 |
| EcoRII | CCWGG | 2 cut(s) | 289, 575 |
| Esp3I | CGTCTC | 1 cut(s) | 386 |
| FaeI | CATG | 5 cut(s) | 364, 713, 722, 911, 940 |
| FaqI | GGGAC | 3 cut(s) | 601, 720, 834 |
| FatI | CATG | 5 cut(s) | 360, 709, 718, 907, 936 |
| Fnu4HI | GCNGC | 1 cut(s) | 780 |
| FokI | GGATG | 2 cut(s) | 257, 958 |
| Fsp4HI | GCNGC | 1 cut(s) | 780 |
| FspBI | CTAG | 2 cut(s) | 195, 464 |
| GlaI | GCGC | 1 cut(s) | 368 |
| GluI | GCNGC | 1 cut(s) | 780 |
| GsaI | CCCAGC | 1 cut(s) | 208 |
| HaeIII | GGCC | 1 cut(s) | 580 |
| HhaI | GCGC | 1 cut(s) | 369 |
| Hin1II | CATG | 5 cut(s) | 364, 713, 722, 911, 940 |
| Hin6I | GCGC | 1 cut(s) | 367 |
| HinP1I | GCGC | 1 cut(s) | 367 |
| HincII | GTYRAC | 1 cut(s) | 748 |
| HindII | GTYRAC | 1 cut(s) | 748 |
| HinfI | GANTC | 8 cut(s) | 38, 315, 430, 501, 556, 838, 851, 890 |
| HphI | GGTGA | 2 cut(s) | 163, 856 |
| Hpy166II | GTNNAC | 1 cut(s) | 748 |
| Hpy188I | TCNGA | 4 cut(s) | 43, 73, 177, 843 |
| Hpy188III | TCNNGA | 5 cut(s) | 275, 562, 602, 932, 956 |
| Hpy8I | GTNNAC | 1 cut(s) | 748 |
| HpyAV | CCTTC | 4 cut(s) | 38, 224, 390, 986 |
| HpyCH4III | ACNGT | 3 cut(s) | 240, 825, 862 |
| HpyCH4IV | ACGT | 5 cut(s) | 259, 379, 661, 679, 697 |
| HpyCH4V | TGCA | 5 cut(s) | 532, 647, 692, 733, 907 |
| HpyF3I | CTNAG | 5 cut(s) | 21, 40, 176, 540, 805 |
| HpySE526I | ACGT | 5 cut(s) | 259, 379, 661, 679, 697 |
| Hsp92II | CATG | 5 cut(s) | 364, 713, 722, 911, 940 |
| HspAI | GCGC | 1 cut(s) | 367 |
| Kzo9I | GATC | 8 cut(s) | 7, 121, 190, 420, 667, 715, 843, 942 |
| LmnI | GCTCC | 1 cut(s) | 291 |
| Lsp1109I | GCAGC | 1 cut(s) | 791 |
| LweI | GCATC | 3 cut(s) | 151, 235, 720 |
| MaeI | CTAG | 2 cut(s) | 195, 464 |
| MaeII | ACGT | 5 cut(s) | 259, 379, 661, 679, 697 |
| MaeIII | GTNAC | 2 cut(s) | 862, 932 |
| MalI | GATC | 8 cut(s) | 9, 123, 192, 422, 669, 717, 845, 944 |
| MboI | GATC | 8 cut(s) | 7, 121, 190, 420, 667, 715, 843, 942 |
| MboII | GAAGA | 2 cut(s) | 235, 422 |
| MluCI | AATT | 8 cut(s) | 168, 385, 448, 518, 549, 597, 610, 784 |
| MlyI | GAGTC | 3 cut(s) | 32, 424, 884 |
| MmeI | TCCRAC | 2 cut(s) | 35, 462 |
| MnlI | CCTC | 7 cut(s) | 171, 598, 786, 839, 920, 1037, 1041 |
| MseI | TTAA | 4 cut(s) | 167, 252, 371, 513 |
| MspR9I | CCNGG | 2 cut(s) | 291, 577 |
| MvaI | CCWGG | 2 cut(s) | 291, 577 |
| NdeII | GATC | 8 cut(s) | 7, 121, 190, 420, 667, 715, 843, 942 |
| NlaIII | CATG | 5 cut(s) | 364, 713, 722, 911, 940 |
| NlaIV | GGNNCC | 1 cut(s) | 923 |
| NmuCI | GTSAC | 2 cut(s) | 862, 932 |
| NspI | RCATGY | 1 cut(s) | 364 |
| NspV | TTCGAA | 1 cut(s) | 983 |
| PciI | ACATGT | 1 cut(s) | 360 |
| PfeI | GAWTC | 5 cut(s) | 315, 501, 556, 838, 851 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 64 |
| PkrI | GCNGC | 1 cut(s) | 781 |
| PleI | GAGTC | 3 cut(s) | 32, 424, 884 |
| PmaCI | CACGTG | 2 cut(s) | 680, 698 |
| PmlI | CACGTG | 2 cut(s) | 680, 698 |
| PpsI | GAGTC | 3 cut(s) | 32, 424, 884 |
| Ppu21I | YACGTR | 2 cut(s) | 680, 698 |
| PscI | ACATGT | 1 cut(s) | 360 |
| Psp6I | CCWGG | 2 cut(s) | 289, 575 |
| PspCI | CACGTG | 2 cut(s) | 680, 698 |
| PspFI | CCCAGC | 1 cut(s) | 204 |
| PspGI | CCWGG | 2 cut(s) | 289, 575 |
| PspN4I | GGNNCC | 1 cut(s) | 923 |
| PspPI | GGNCC | 1 cut(s) | 579 |
| RsaI | GTAC | 4 cut(s) | 56, 212, 827, 1013 |
| RsaNI | GTAC | 4 cut(s) | 55, 211, 826, 1012 |
| SaqAI | TTAA | 4 cut(s) | 167, 252, 371, 513 |
| SatI | GCNGC | 1 cut(s) | 780 |
| Sau3AI | GATC | 8 cut(s) | 7, 121, 190, 420, 667, 715, 843, 942 |
| Sau96I | GGNCC | 1 cut(s) | 579 |
| SchI | GAGTC | 3 cut(s) | 32, 424, 884 |
| ScrFI | CCNGG | 2 cut(s) | 291, 577 |
| SfaNI | GCATC | 3 cut(s) | 151, 235, 720 |
| SfcI | CTRYAG | 1 cut(s) | 763 |
| SfuI | TTCGAA | 1 cut(s) | 983 |
| SmlI | CTYRAG | 1 cut(s) | 50 |
| SmoI | CTYRAG | 1 cut(s) | 50 |
| Sse9I | AATT | 8 cut(s) | 168, 385, 448, 518, 549, 597, 610, 784 |
| SsiI | CCGC | 2 cut(s) | 319, 913 |
| SspMI | CTAG | 2 cut(s) | 195, 464 |
| StyD4I | CCNGG | 2 cut(s) | 289, 575 |
| TaaI | ACNGT | 3 cut(s) | 240, 825, 862 |
| TaiI | ACGT | 5 cut(s) | 262, 382, 664, 682, 700 |
| TaqI | TCGA | 2 cut(s) | 16, 983 |
| TasI | AATT | 8 cut(s) | 168, 385, 448, 518, 549, 597, 610, 784 |
| TfiI | GAWTC | 5 cut(s) | 315, 501, 556, 838, 851 |
| Tru1I | TTAA | 4 cut(s) | 167, 252, 371, 513 |
| Tru9I | TTAA | 4 cut(s) | 167, 252, 371, 513 |
| TscAI | CASTG | 5 cut(s) | 112, 243, 311, 589, 892 |
| TseFI | GTSAC | 2 cut(s) | 862, 932 |
| TseI | GCWGC | 1 cut(s) | 779 |
| Tsp45I | GTSAC | 2 cut(s) | 862, 932 |
| TspDTI | ATGAA | 5 cut(s) | 150, 638, 768, 888, 1002 |
| TspGWI | ACGGA | 2 cut(s) | 935, 982 |
| TspRI | CASTG | 5 cut(s) | 112, 243, 311, 589, 892 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 64 |
| XapI | RAATTY | 1 cut(s) | 597 |
| XceI | RCATGY | 1 cut(s) | 364 |
| XspI | CTAG | 2 cut(s) | 195, 464 |