No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 70 |
| AccB1I | GGYRCC | 1 cut(s) | 262 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 409 |
| AccI | GTMKAC | 1 cut(s) | 362 |
| AccII | CGCG | 1 cut(s) | 245 |
| AciI | CCGC | 1 cut(s) | 709 |
| AclWI | GGATC | 3 cut(s) | 418, 575, 805 |
| AcoI | YGGCCR | 2 cut(s) | 158, 386 |
| AcsI | RAATTY | 2 cut(s) | 101, 274 |
| AcuI | CTGAAG | 2 cut(s) | 590, 824 |
| AcyI | GRCGYC | 1 cut(s) | 67 |
| AfaI | GTAC | 1 cut(s) | 602 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 292, 327, 334, 395, 409, 696 |
| AflII | CTTAAG | 1 cut(s) | 641 |
| AgeI | ACCGGT | 1 cut(s) | 327 |
| AgsI | TTSAA | 4 cut(s) | 187, 568, 613, 742 |
| AhdI | GACNNNNNGTC | 3 cut(s) | 233, 369, 396 |
| AjiI | CACGTC | 1 cut(s) | 770 |
| AjnI | CCWGG | 1 cut(s) | 408 |
| AluBI | AGCT | 1 cut(s) | 588 |
| AluI | AGCT | 1 cut(s) | 588 |
| Alw26I | GTCTC | 2 cut(s) | 315, 776 |
| AlwI | GGATC | 3 cut(s) | 418, 575, 805 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 59, 409 |
| Ama87I | CYCGRG | 1 cut(s) | 75 |
| AoxI | GGCC | 2 cut(s) | 158, 386 |
| ApoI | RAATTY | 2 cut(s) | 101, 274 |
| AsiGI | ACCGGT | 1 cut(s) | 327 |
| AspLEI | GCGC | 3 cut(s) | 206, 247, 759 |
| AspS9I | GGNCC | 1 cut(s) | 527 |
| AsuC2I | CCSGG | 1 cut(s) | 239 |
| AsuHPI | GGTGA | 5 cut(s) | 317, 338, 365, 392, 553 |
| AvaI | CYCGRG | 1 cut(s) | 75 |
| AvaII | GGWCC | 1 cut(s) | 527 |
| BaeGI | GKGCMC | 1 cut(s) | 267 |
| BanI | GGYRCC | 1 cut(s) | 262 |
| BbsI | GAAGAC | 2 cut(s) | 620, 824 |
| BccI | CCATC | 3 cut(s) | 411, 518, 538 |
| BceAI | ACGGC | 2 cut(s) | 103, 173 |
| BciT130I | CCWGG | 1 cut(s) | 410 |
| BclI | TGATCA | 1 cut(s) | 25 |
| BcnI | CCSGG | 1 cut(s) | 239 |
| BcoDI | GTCTC | 2 cut(s) | 315, 776 |
| BfaI | CTAG | 2 cut(s) | 585, 853 |
| BfrI | CTTAAG | 1 cut(s) | 641 |
| BglII | AGATCT | 1 cut(s) | 19 |
| Bme1390I | CCNGG | 2 cut(s) | 239, 410 |
| Bme18I | GGWCC | 1 cut(s) | 527 |
| BmeRI | GACNNNNNGTC | 3 cut(s) | 233, 369, 396 |
| BmeT110I | CYCGRG | 1 cut(s) | 75 |
| BmgBI | CACGTC | 1 cut(s) | 770 |
| BmgT120I | GGNCC | 1 cut(s) | 527 |
| BmiI | GGNNCC | 1 cut(s) | 264 |
| BmrFI | CCNGG | 2 cut(s) | 239, 410 |
| BmsI | GCATC | 2 cut(s) | 154, 625 |
| BpiI | GAAGAC | 2 cut(s) | 620, 824 |
| BpmI | CTGGAG | 2 cut(s) | 132, 356 |
| BpuMI | CCSGG | 1 cut(s) | 239 |
| BsaBI | GATNNNNATC | 2 cut(s) | 24, 321 |
| BsaHI | GRCGYC | 1 cut(s) | 67 |
| BsaJI | CCNNGG | 2 cut(s) | 356, 383 |
| BsaWI | WCCGGW | 1 cut(s) | 327 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 292, 327, 334, 395, 409, 696 |
| Bse118I | RCCGGY | 1 cut(s) | 327 |
| Bse1I | ACTGG | 3 cut(s) | 233, 373, 400 |
| Bse8I | GATNNNNATC | 2 cut(s) | 24, 321 |
| BseBI | CCWGG | 1 cut(s) | 410 |
| BseDI | CCNNGG | 2 cut(s) | 356, 383 |
| BseGI | GGATG | 1 cut(s) | 640 |
| BseJI | GATNNNNATC | 2 cut(s) | 24, 321 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 292, 327, 334, 395, 409, 696 |
| BseNI | ACTGG | 3 cut(s) | 233, 373, 400 |
| BseSI | GKGCMC | 1 cut(s) | 267 |
| BsgI | GTGCAG | 1 cut(s) | 113 |
| Bsh1236I | CGCG | 1 cut(s) | 245 |
| Bsh1285I | CGRYCG | 3 cut(s) | 243, 332, 362 |
| BshFI | GGCC | 2 cut(s) | 160, 388 |
| BshNI | GGYRCC | 1 cut(s) | 262 |
| BshTI | ACCGGT | 1 cut(s) | 327 |
| BsiEI | CGRYCG | 3 cut(s) | 243, 332, 362 |
| BsiHKCI | CYCGRG | 1 cut(s) | 75 |
| BsiSI | CCGG | 3 cut(s) | 239, 286, 328 |
| BslFI | GGGAC | 2 cut(s) | 221, 660 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 292, 327, 334, 395, 409, 696 |
| BsmAI | GTCTC | 2 cut(s) | 315, 776 |
| BsmBI | CGTCTC | 1 cut(s) | 776 |
| BsmFI | GGGAC | 2 cut(s) | 221, 660 |
| BsnI | GGCC | 2 cut(s) | 160, 388 |
| BsoBI | CYCGRG | 1 cut(s) | 75 |
| Bsp1286I | GDGCHC | 1 cut(s) | 267 |
| Bsp143I | GATC | 8 cut(s) | 19, 25, 113, 256, 322, 423, 580, 810 |
| Bsp19I | CCATGG | 1 cut(s) | 383 |
| BspACI | CCGC | 1 cut(s) | 709 |
| BspANI | GGCC | 2 cut(s) | 160, 388 |
| BspFNI | CGCG | 1 cut(s) | 245 |
| BspLI | GGNNCC | 1 cut(s) | 264 |
| BspPI | GGATC | 3 cut(s) | 418, 575, 805 |
| BspT107I | GGYRCC | 1 cut(s) | 262 |
| BspTI | CTTAAG | 1 cut(s) | 641 |
| BsrFI | RCCGGY | 1 cut(s) | 327 |
| BsrI | ACTGG | 3 cut(s) | 233, 373, 400 |
| BssAI | RCCGGY | 1 cut(s) | 327 |
| BssECI | CCNNGG | 2 cut(s) | 356, 383 |
| BssMI | GATC | 8 cut(s) | 19, 25, 113, 256, 322, 423, 580, 810 |
| BssNI | GRCGYC | 1 cut(s) | 67 |
| BssT1I | CCWWGG | 1 cut(s) | 383 |
| Bst2UI | CCWGG | 1 cut(s) | 410 |
| Bst4CI | ACNGT | 5 cut(s) | 123, 360, 432, 443, 691 |
| BstACI | GRCGYC | 1 cut(s) | 67 |
| BstAFI | CTTAAG | 1 cut(s) | 641 |
| BstC8I | GCNNGC | 1 cut(s) | 713 |
| BstDSI | CCRYGG | 2 cut(s) | 356, 383 |
| BstEII | GGTNACC | 3 cut(s) | 344, 371, 398 |
| BstF5I | GGATG | 1 cut(s) | 640 |
| BstFNI | CGCG | 1 cut(s) | 245 |
| BstHHI | GCGC | 3 cut(s) | 206, 247, 759 |
| BstKTI | GATC | 8 cut(s) | 22, 28, 116, 259, 325, 426, 583, 813 |
| BstMAI | GTCTC | 2 cut(s) | 315, 776 |
| BstMBI | GATC | 8 cut(s) | 19, 25, 113, 256, 322, 423, 580, 810 |
| BstMCI | CGRYCG | 3 cut(s) | 243, 332, 362 |
| BstNI | CCWGG | 1 cut(s) | 410 |
| BstPI | GGTNACC | 3 cut(s) | 344, 371, 398 |
| BstSCI | CCNGG | 2 cut(s) | 237, 408 |
| BstSLI | GKGCMC | 1 cut(s) | 267 |
| BstUI | CGCG | 1 cut(s) | 245 |
| BstV2I | GAAGAC | 2 cut(s) | 620, 824 |
| BstX2I | RGATCY | 1 cut(s) | 19 |
| BstYI | RGATCY | 1 cut(s) | 19 |
| BsuRI | GGCC | 2 cut(s) | 160, 388 |
| BtgI | CCRYGG | 2 cut(s) | 356, 383 |
| BtrI | CACGTC | 1 cut(s) | 770 |
| BtsCI | GGATG | 1 cut(s) | 640 |
| BtsI | GCAGTG | 1 cut(s) | 486 |
| BtsIMutI | CAGTG | 5 cut(s) | 366, 393, 486, 507, 701 |
| Cac8I | GCNNGC | 1 cut(s) | 713 |
| CaiI | CAGNNNCTG | 2 cut(s) | 59, 409 |
| CfoI | GCGC | 3 cut(s) | 206, 247, 759 |
| Cfr10I | RCCGGY | 1 cut(s) | 327 |
| Cfr13I | GGNCC | 1 cut(s) | 527 |
| CseI | GACGC | 1 cut(s) | 23 |
| Csp6I | GTAC | 1 cut(s) | 601 |
| CspAI | ACCGGT | 1 cut(s) | 327 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 763, 798 |
| CviAII | CATG | 1 cut(s) | 384 |
| CviJI | RGCY | 9 cut(s) | 31, 56, 152, 160, 285, 388, 408, 421, 588 |
| CviKI_1 | RGCY | 9 cut(s) | 31, 56, 152, 160, 285, 388, 408, 421, 588 |
| CviQI | GTAC | 1 cut(s) | 601 |
| DpnI | GATC | 8 cut(s) | 21, 27, 115, 258, 324, 425, 582, 812 |
| DpnII | GATC | 8 cut(s) | 19, 25, 113, 256, 322, 423, 580, 810 |
| DriI | GACNNNNNGTC | 3 cut(s) | 233, 369, 396 |
| EaeI | YGGCCR | 2 cut(s) | 158, 386 |
| Eam1105I | GACNNNNNGTC | 3 cut(s) | 233, 369, 396 |
| Eco130I | CCWWGG | 1 cut(s) | 383 |
| Eco47I | GGWCC | 1 cut(s) | 527 |
| Eco57I | CTGAAG | 2 cut(s) | 590, 824 |
| Eco88I | CYCGRG | 1 cut(s) | 75 |
| Eco91I | GGTNACC | 3 cut(s) | 344, 371, 398 |
| EcoO65I | GGTNACC | 3 cut(s) | 344, 371, 398 |
| EcoRI | GAATTC | 1 cut(s) | 101 |
| EcoRII | CCWGG | 1 cut(s) | 408 |
| EcoT14I | CCWWGG | 1 cut(s) | 383 |
| ErhI | CCWWGG | 1 cut(s) | 383 |
| Esp3I | CGTCTC | 1 cut(s) | 776 |
| FaeI | CATG | 1 cut(s) | 387 |
| FaiI | YATR | 8 cut(s) | 119, 385, 576, 669, 723, 752, 809, 850 |
| FaqI | GGGAC | 2 cut(s) | 221, 660 |
| FatI | CATG | 1 cut(s) | 383 |
| FbaI | TGATCA | 1 cut(s) | 25 |
| FblI | GTMKAC | 1 cut(s) | 362 |
| FokI | GGATG | 1 cut(s) | 647 |
| FspBI | CTAG | 2 cut(s) | 585, 853 |
| GlaI | GCGC | 3 cut(s) | 205, 246, 758 |
| GsuI | CTGGAG | 2 cut(s) | 132, 356 |
| HaeIII | GGCC | 2 cut(s) | 160, 388 |
| HapII | CCGG | 3 cut(s) | 239, 286, 328 |
| HgaI | GACGC | 1 cut(s) | 23 |
| HhaI | GCGC | 3 cut(s) | 206, 247, 759 |
| Hin1I | GRCGYC | 1 cut(s) | 67 |
| Hin1II | CATG | 1 cut(s) | 387 |
| Hin6I | GCGC | 3 cut(s) | 204, 245, 757 |
| HinP1I | GCGC | 3 cut(s) | 204, 245, 757 |
| HincII | GTYRAC | 1 cut(s) | 363 |
| HindII | GTYRAC | 1 cut(s) | 363 |
| HinfI | GANTC | 9 cut(s) | 78, 95, 289, 296, 317, 338, 352, 379, 820 |
| HpaII | CCGG | 3 cut(s) | 239, 286, 328 |
| HphI | GGTGA | 5 cut(s) | 317, 338, 365, 392, 553 |
| Hpy166II | GTNNAC | 3 cut(s) | 356, 363, 674 |
| Hpy188I | TCNGA | 5 cut(s) | 100, 113, 195, 322, 535 |
| Hpy188III | TCNNGA | 5 cut(s) | 23, 75, 293, 314, 814 |
| Hpy8I | GTNNAC | 3 cut(s) | 356, 363, 674 |
| Hpy99I | CGWCG | 1 cut(s) | 69 |
| HpyAV | CCTTC | 1 cut(s) | 614 |
| HpyCH4III | ACNGT | 5 cut(s) | 123, 360, 432, 443, 691 |
| HpyCH4IV | ACGT | 4 cut(s) | 67, 592, 649, 769 |
| HpyCH4V | TGCA | 1 cut(s) | 130 |
| HpySE526I | ACGT | 4 cut(s) | 67, 592, 649, 769 |
| Hsp92I | GRCGYC | 1 cut(s) | 67 |
| Hsp92II | CATG | 1 cut(s) | 387 |
| HspAI | GCGC | 3 cut(s) | 204, 245, 757 |
| Ksp22I | TGATCA | 1 cut(s) | 25 |
| Kzo9I | GATC | 8 cut(s) | 19, 25, 113, 256, 322, 423, 580, 810 |
| LweI | GCATC | 2 cut(s) | 154, 625 |
| MaeI | CTAG | 2 cut(s) | 585, 853 |
| MaeII | ACGT | 4 cut(s) | 67, 592, 649, 769 |
| MaeIII | GTNAC | 3 cut(s) | 344, 371, 398 |
| MalI | GATC | 8 cut(s) | 21, 27, 115, 258, 324, 425, 582, 812 |
| MboI | GATC | 8 cut(s) | 19, 25, 113, 256, 322, 423, 580, 810 |
| MboII | GAAGA | 3 cut(s) | 625, 812, 829 |
| MflI | RGATCY | 1 cut(s) | 19 |
| MhlI | GDGCHC | 1 cut(s) | 267 |
| MluCI | AATT | 3 cut(s) | 101, 274, 775 |
| MlyI | GAGTC | 5 cut(s) | 87, 347, 361, 388, 814 |
| MmeI | TCCRAC | 2 cut(s) | 173, 451 |
| MnlI | CCTC | 4 cut(s) | 85, 222, 478, 532 |
| MseI | TTAA | 2 cut(s) | 557, 642 |
| MspCI | CTTAAG | 1 cut(s) | 641 |
| MspI | CCGG | 3 cut(s) | 239, 286, 328 |
| MspR9I | CCNGG | 2 cut(s) | 239, 410 |
| MvaI | CCWGG | 1 cut(s) | 410 |
| MvnI | CGCG | 1 cut(s) | 245 |
| NciI | CCSGG | 1 cut(s) | 239 |
| NcoI | CCATGG | 1 cut(s) | 383 |
| NdeII | GATC | 8 cut(s) | 19, 25, 113, 256, 322, 423, 580, 810 |
| NlaIII | CATG | 1 cut(s) | 387 |
| NlaIV | GGNNCC | 1 cut(s) | 264 |
| NmuCI | GTSAC | 3 cut(s) | 344, 371, 398 |
| PaeR7I | CTCGAG | 1 cut(s) | 75 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 73 |
| PfeI | GAWTC | 4 cut(s) | 95, 289, 296, 317 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 409 |
| PinAI | ACCGGT | 1 cut(s) | 327 |
| PleI | GAGTC | 5 cut(s) | 86, 346, 360, 387, 814 |
| PpsI | GAGTC | 5 cut(s) | 86, 346, 360, 387, 814 |
| Psp6I | CCWGG | 1 cut(s) | 408 |
| PspEI | GGTNACC | 3 cut(s) | 344, 371, 398 |
| PspGI | CCWGG | 1 cut(s) | 408 |
| PspN4I | GGNNCC | 1 cut(s) | 264 |
| PspPI | GGNCC | 1 cut(s) | 527 |
| PstNI | CAGNNNCTG | 2 cut(s) | 59, 409 |
| PsuI | RGATCY | 1 cut(s) | 19 |
| RsaI | GTAC | 1 cut(s) | 602 |
| RsaNI | GTAC | 1 cut(s) | 601 |
| SalI | GTCGAC | 1 cut(s) | 361 |
| SaqAI | TTAA | 2 cut(s) | 557, 642 |
| Sau3AI | GATC | 8 cut(s) | 19, 25, 113, 256, 322, 423, 580, 810 |
| Sau96I | GGNCC | 1 cut(s) | 527 |
| SchI | GAGTC | 5 cut(s) | 87, 347, 361, 388, 814 |
| ScrFI | CCNGG | 2 cut(s) | 239, 410 |
| SduI | GDGCHC | 1 cut(s) | 267 |
| SetI | ASST | 9 cut(s) | 70, 214, 543, 590, 595, 606, 630, 652, 772 |
| SfaNI | GCATC | 2 cut(s) | 154, 625 |
| Sfr274I | CTCGAG | 1 cut(s) | 75 |
| SinI | GGWCC | 1 cut(s) | 527 |
| SlaI | CTCGAG | 1 cut(s) | 75 |
| SmlI | CTYRAG | 2 cut(s) | 75, 641 |
| SmoI | CTYRAG | 2 cut(s) | 75, 641 |
| Sse9I | AATT | 3 cut(s) | 101, 274, 775 |
| SsiI | CCGC | 1 cut(s) | 709 |
| SspMI | CTAG | 2 cut(s) | 585, 853 |
| StyD4I | CCNGG | 2 cut(s) | 237, 408 |
| StyI | CCWWGG | 1 cut(s) | 383 |
| TaaI | ACNGT | 5 cut(s) | 123, 360, 432, 443, 691 |
| TaiI | ACGT | 4 cut(s) | 70, 595, 652, 772 |
| TaqI | TCGA | 5 cut(s) | 6, 64, 76, 362, 453 |
| TasI | AATT | 3 cut(s) | 101, 274, 775 |
| TfiI | GAWTC | 4 cut(s) | 95, 289, 296, 317 |
| Tru1I | TTAA | 2 cut(s) | 557, 642 |
| Tru9I | TTAA | 2 cut(s) | 557, 642 |
| TscAI | CASTG | 5 cut(s) | 373, 400, 493, 514, 701 |
| TseFI | GTSAC | 3 cut(s) | 344, 371, 398 |
| Tsp45I | GTSAC | 3 cut(s) | 344, 371, 398 |
| TspDTI | ATGAA | 3 cut(s) | 653, 717, 816 |
| TspRI | CASTG | 5 cut(s) | 373, 400, 493, 514, 701 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 409 |
| Vha464I | CTTAAG | 1 cut(s) | 641 |
| VpaK11BI | GGWCC | 1 cut(s) | 527 |
| XapI | RAATTY | 2 cut(s) | 101, 274 |
| XhoI | CTCGAG | 1 cut(s) | 75 |
| XmiI | GTMKAC | 1 cut(s) | 362 |
| XspI | CTAG | 2 cut(s) | 585, 853 |
| ZraI | GACGTC | 1 cut(s) | 68 |