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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 206, 311 |
| Acc36I | ACCTGC | 2 cut(s) | 206, 311 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 470, 777 |
| AccII | CGCG | 5 cut(s) | 118, 342, 344, 412, 476 |
| AciI | CCGC | 4 cut(s) | 118, 262, 476, 790 |
| AclWI | GGATC | 1 cut(s) | 533 |
| AcsI | RAATTY | 3 cut(s) | 4, 856, 1071 |
| AcuI | CTGAAG | 1 cut(s) | 906 |
| AcvI | CACGTG | 1 cut(s) | 1156 |
| AfaI | GTAC | 1 cut(s) | 587 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 470, 595, 777, 1122 |
| AflII | CTTAAG | 1 cut(s) | 722 |
| AflIII | ACRYGT | 1 cut(s) | 831 |
| AgsI | TTSAA | 5 cut(s) | 522, 694, 823, 985, 1039 |
| AjiI | CACGTC | 2 cut(s) | 851, 1136 |
| AjnI | CCWGG | 1 cut(s) | 1049 |
| AluBI | AGCT | 2 cut(s) | 389, 926 |
| AluI | AGCT | 2 cut(s) | 389, 926 |
| Alw26I | GTCTC | 2 cut(s) | 624, 1012 |
| AlwI | GGATC | 1 cut(s) | 533 |
| Ama87I | CYCGRG | 1 cut(s) | 1076 |
| AoxI | GGCC | 5 cut(s) | 142, 428, 459, 477, 1052 |
| ApeKI | GCWGC | 8 cut(s) | 201, 290, 314, 364, 367, 382, 404, 407 |
| ApoI | RAATTY | 3 cut(s) | 4, 856, 1071 |
| AspLEI | GCGC | 7 cut(s) | 314, 342, 344, 412, 414, 476, 840 |
| AspS9I | GGNCC | 7 cut(s) | 57, 91, 120, 429, 460, 677, 1053 |
| AsuC2I | CCSGG | 1 cut(s) | 77 |
| AsuHPI | GGTGA | 4 cut(s) | 54, 125, 187, 217 |
| AvaI | CYCGRG | 1 cut(s) | 1076 |
| AvaII | GGWCC | 4 cut(s) | 57, 91, 120, 677 |
| AxyI | CCTNAGG | 1 cut(s) | 273 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 569, 602 |
| BbrPI | CACGTG | 1 cut(s) | 1156 |
| BbsI | GAAGAC | 2 cut(s) | 510, 701 |
| BbvI | GCAGC | 8 cut(s) | 213, 301, 302, 376, 379, 391, 394, 419 |
| BccI | CCATC | 4 cut(s) | 506, 597, 653, 668 |
| BceAI | ACGGC | 2 cut(s) | 325, 495 |
| BciT130I | CCWGG | 1 cut(s) | 1051 |
| BcnI | CCSGG | 1 cut(s) | 77 |
| BcoDI | GTCTC | 2 cut(s) | 624, 1012 |
| BfaI | CTAG | 2 cut(s) | 452, 935 |
| BfmI | CTRYAG | 2 cut(s) | 199, 405 |
| BfoI | RGCGCY | 1 cut(s) | 315 |
| BfrI | CTTAAG | 1 cut(s) | 722 |
| BfuAI | ACCTGC | 2 cut(s) | 206, 311 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 623 |
| BisI | GCNGC | 9 cut(s) | 202, 291, 315, 365, 368, 383, 405, 408, 477 |
| BlsI | GCNGC | 9 cut(s) | 203, 292, 316, 366, 369, 384, 406, 409, 478 |
| Bme1390I | CCNGG | 2 cut(s) | 77, 1051 |
| Bme18I | GGWCC | 4 cut(s) | 57, 91, 120, 677 |
| BmeT110I | CYCGRG | 1 cut(s) | 1076 |
| BmgBI | CACGTC | 2 cut(s) | 851, 1136 |
| BmgT120I | GGNCC | 7 cut(s) | 57, 91, 120, 429, 460, 677, 1053 |
| BmiI | GGNNCC | 2 cut(s) | 431, 462 |
| BmrFI | CCNGG | 2 cut(s) | 77, 1051 |
| BmrI | ACTGGG | 1 cut(s) | 1068 |
| BmsI | GCATC | 1 cut(s) | 706 |
| BmuI | ACTGGG | 1 cut(s) | 1068 |
| BpiI | GAAGAC | 2 cut(s) | 510, 701 |
| BpmI | CTGGAG | 2 cut(s) | 142, 404 |
| BpuEI | CTTGAG | 1 cut(s) | 602 |
| BpuMI | CCSGG | 1 cut(s) | 77 |
| BsaAI | YACGTR | 1 cut(s) | 1156 |
| BsaJI | CCNNGG | 4 cut(s) | 425, 433, 456, 1077 |
| BsaWI | WCCGGW | 1 cut(s) | 93 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 470, 595, 777, 1122 |
| Bse118I | RCCGGY | 1 cut(s) | 135 |
| Bse1I | ACTGG | 6 cut(s) | 159, 187, 217, 475, 943, 1063 |
| Bse21I | CCTNAGG | 1 cut(s) | 273 |
| Bse3DI | GCAATG | 1 cut(s) | 752 |
| BseBI | CCWGG | 1 cut(s) | 1051 |
| BseDI | CCNNGG | 4 cut(s) | 425, 433, 456, 1077 |
| BseGI | GGATG | 3 cut(s) | 351, 444, 721 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 470, 595, 777, 1122 |
| BseMI | GCAATG | 1 cut(s) | 752 |
| BseMII | CTCAG | 4 cut(s) | 287, 543, 585, 1005 |
| BseNI | ACTGG | 6 cut(s) | 159, 187, 217, 475, 943, 1063 |
| BsePI | GCGCGC | 2 cut(s) | 340, 410 |
| BseXI | GCAGC | 8 cut(s) | 213, 301, 302, 376, 379, 391, 394, 419 |
| BseYI | CCCAGC | 3 cut(s) | 252, 326, 355 |
| Bsh1236I | CGCG | 5 cut(s) | 118, 342, 344, 412, 476 |
| Bsh1285I | CGRYCG | 1 cut(s) | 377 |
| BshFI | GGCC | 5 cut(s) | 144, 430, 461, 479, 1054 |
| BsiEI | CGRYCG | 1 cut(s) | 377 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1076 |
| BsiSI | CCGG | 3 cut(s) | 77, 94, 136 |
| BslFI | GGGAC | 4 cut(s) | 149, 741, 1075, 1196 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 470, 595, 777, 1122 |
| BsmAI | GTCTC | 2 cut(s) | 624, 1012 |
| BsmFI | GGGAC | 4 cut(s) | 149, 741, 1075, 1196 |
| BsnI | GGCC | 5 cut(s) | 144, 430, 461, 479, 1054 |
| BsoBI | CYCGRG | 1 cut(s) | 1076 |
| Bsp143I | GATC | 5 cut(s) | 169, 192, 374, 538, 891 |
| BspACI | CCGC | 4 cut(s) | 118, 262, 476, 790 |
| BspANI | GGCC | 5 cut(s) | 144, 430, 461, 479, 1054 |
| BspCNI | CTCAG | 4 cut(s) | 286, 544, 584, 1006 |
| BspFNI | CGCG | 5 cut(s) | 118, 342, 344, 412, 476 |
| BspHI | TCATGA | 1 cut(s) | 745 |
| BspLI | GGNNCC | 2 cut(s) | 431, 462 |
| BspMAI | CTGCAG | 2 cut(s) | 203, 409 |
| BspMI | ACCTGC | 2 cut(s) | 206, 311 |
| BspPI | GGATC | 1 cut(s) | 533 |
| BspTI | CTTAAG | 1 cut(s) | 722 |
| BsrDI | GCAATG | 1 cut(s) | 752 |
| BsrFI | RCCGGY | 1 cut(s) | 135 |
| BsrI | ACTGG | 6 cut(s) | 159, 187, 217, 475, 943, 1063 |
| BssAI | RCCGGY | 1 cut(s) | 135 |
| BssECI | CCNNGG | 4 cut(s) | 425, 433, 456, 1077 |
| BssHII | GCGCGC | 2 cut(s) | 340, 410 |
| BssMI | GATC | 5 cut(s) | 169, 192, 374, 538, 891 |
| BssT1I | CCWWGG | 1 cut(s) | 425 |
| Bst2UI | CCWGG | 1 cut(s) | 1051 |
| Bst4CI | ACNGT | 2 cut(s) | 681, 711 |
| BstAFI | CTTAAG | 1 cut(s) | 722 |
| BstBAI | YACGTR | 1 cut(s) | 1156 |
| BstC8I | GCNNGC | 4 cut(s) | 342, 387, 412, 440 |
| BstDEI | CTNAG | 4 cut(s) | 273, 552, 571, 1014 |
| BstDSI | CCRYGG | 1 cut(s) | 433 |
| BstF5I | GGATG | 3 cut(s) | 351, 444, 721 |
| BstFNI | CGCG | 5 cut(s) | 118, 342, 344, 412, 476 |
| BstH2I | RGCGCY | 1 cut(s) | 315 |
| BstHHI | GCGC | 7 cut(s) | 314, 342, 344, 412, 414, 476, 840 |
| BstKTI | GATC | 5 cut(s) | 172, 195, 377, 541, 894 |
| BstMAI | GTCTC | 2 cut(s) | 624, 1012 |
| BstMBI | GATC | 5 cut(s) | 169, 192, 374, 538, 891 |
| BstMCI | CGRYCG | 1 cut(s) | 377 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 275, 290, 623 |
| BstNI | CCWGG | 1 cut(s) | 1051 |
| BstNSI | RCATGY | 1 cut(s) | 835 |
| BstSCI | CCNGG | 2 cut(s) | 75, 1049 |
| BstSFI | CTRYAG | 2 cut(s) | 199, 405 |
| BstUI | CGCG | 5 cut(s) | 118, 342, 344, 412, 476 |
| BstV1I | GCAGC | 8 cut(s) | 213, 301, 302, 376, 379, 391, 394, 419 |
| BstV2I | GAAGAC | 2 cut(s) | 510, 701 |
| Bsu36I | CCTNAGG | 1 cut(s) | 273 |
| BsuRI | GGCC | 5 cut(s) | 144, 430, 461, 479, 1054 |
| BtgI | CCRYGG | 1 cut(s) | 433 |
| BtrI | CACGTC | 2 cut(s) | 851, 1136 |
| BtsCI | GGATG | 3 cut(s) | 351, 444, 721 |
| BtsI | GCAGTG | 1 cut(s) | 636 |
| BtsIMutI | CAGTG | 4 cut(s) | 166, 468, 636, 1056 |
| BveI | ACCTGC | 2 cut(s) | 206, 311 |
| Cac8I | GCNNGC | 4 cut(s) | 342, 387, 412, 440 |
| CciI | TCATGA | 1 cut(s) | 745 |
| CfoI | GCGC | 7 cut(s) | 314, 342, 344, 412, 414, 476, 840 |
| Cfr10I | RCCGGY | 1 cut(s) | 135 |
| Cfr13I | GGNCC | 7 cut(s) | 57, 91, 120, 429, 460, 677, 1053 |
| CseI | GACGC | 1 cut(s) | 124 |
| Csp6I | GTAC | 1 cut(s) | 586 |
| CviAII | CATG | 5 cut(s) | 532, 746, 832, 1174, 1186 |
| CviQI | GTAC | 1 cut(s) | 586 |
| DdeI | CTNAG | 4 cut(s) | 273, 552, 571, 1014 |
| DpnI | GATC | 5 cut(s) | 171, 194, 376, 540, 893 |
| DpnII | GATC | 5 cut(s) | 169, 192, 374, 538, 891 |
| EciI | GGCGGA | 1 cut(s) | 251 |
| Eco130I | CCWWGG | 1 cut(s) | 425 |
| Eco47I | GGWCC | 4 cut(s) | 57, 91, 120, 677 |
| Eco57I | CTGAAG | 1 cut(s) | 906 |
| Eco72I | CACGTG | 1 cut(s) | 1156 |
| Eco81I | CCTNAGG | 1 cut(s) | 273 |
| Eco88I | CYCGRG | 1 cut(s) | 1076 |
| EcoRII | CCWGG | 1 cut(s) | 1049 |
| EcoT14I | CCWWGG | 1 cut(s) | 425 |
| ErhI | CCWWGG | 1 cut(s) | 425 |
| FaeI | CATG | 5 cut(s) | 535, 749, 835, 1177, 1189 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1019, 1051 |
| FaqI | GGGAC | 4 cut(s) | 149, 741, 1075, 1196 |
| FatI | CATG | 5 cut(s) | 531, 745, 831, 1173, 1185 |
| Fnu4HI | GCNGC | 9 cut(s) | 202, 291, 315, 365, 368, 383, 405, 408, 477 |
| FokI | GGATG | 3 cut(s) | 338, 431, 728 |
| Fsp4HI | GCNGC | 9 cut(s) | 202, 291, 315, 365, 368, 383, 405, 408, 477 |
| FspBI | CTAG | 2 cut(s) | 452, 935 |
| GlaI | GCGC | 7 cut(s) | 313, 341, 343, 411, 413, 475, 839 |
| GluI | GCNGC | 9 cut(s) | 202, 291, 315, 365, 368, 383, 405, 408, 477 |
| GsaI | CCCAGC | 3 cut(s) | 256, 330, 359 |
| GsuI | CTGGAG | 2 cut(s) | 142, 404 |
| HaeII | RGCGCY | 1 cut(s) | 315 |
| HaeIII | GGCC | 5 cut(s) | 144, 430, 461, 479, 1054 |
| HapII | CCGG | 3 cut(s) | 77, 94, 136 |
| HgaI | GACGC | 1 cut(s) | 124 |
| HhaI | GCGC | 7 cut(s) | 314, 342, 344, 412, 414, 476, 840 |
| Hin1II | CATG | 5 cut(s) | 535, 749, 835, 1177, 1189 |
| Hin6I | GCGC | 7 cut(s) | 312, 340, 342, 410, 412, 474, 838 |
| HinP1I | GCGC | 7 cut(s) | 312, 340, 342, 410, 412, 474, 838 |
| HindIII | AAGCTT | 1 cut(s) | 387 |
| HinfI | GANTC | 5 cut(s) | 258, 569, 901, 972, 1030 |
| HpaII | CCGG | 3 cut(s) | 77, 94, 136 |
| HphI | GGTGA | 4 cut(s) | 54, 125, 187, 217 |
| Hpy188I | TCNGA | 3 cut(s) | 574, 604, 685 |
| Hpy188III | TCNNGA | 5 cut(s) | 15, 36, 746, 1036, 1076 |
| HpyAV | CCTTC | 3 cut(s) | 238, 569, 861 |
| HpyCH4III | ACNGT | 2 cut(s) | 681, 711 |
| HpyCH4IV | ACGT | 5 cut(s) | 25, 730, 850, 1135, 1155 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 275, 290, 623 |
| HpyF3I | CTNAG | 4 cut(s) | 273, 552, 571, 1014 |
| HpySE526I | ACGT | 5 cut(s) | 25, 730, 850, 1135, 1155 |
| Hsp92II | CATG | 5 cut(s) | 535, 749, 835, 1177, 1189 |
| HspAI | GCGC | 7 cut(s) | 312, 340, 342, 410, 412, 474, 838 |
| Kzo9I | GATC | 5 cut(s) | 169, 192, 374, 538, 891 |
| LmnI | GCTCC | 2 cut(s) | 322, 419 |
| Lsp1109I | GCAGC | 8 cut(s) | 213, 301, 302, 376, 379, 391, 394, 419 |
| LweI | GCATC | 1 cut(s) | 706 |
| MaeI | CTAG | 2 cut(s) | 452, 935 |
| MaeII | ACGT | 5 cut(s) | 25, 730, 850, 1135, 1155 |
| MalI | GATC | 5 cut(s) | 171, 194, 376, 540, 893 |
| MboI | GATC | 5 cut(s) | 169, 192, 374, 538, 891 |
| MboII | GAAGA | 4 cut(s) | 493, 510, 706, 893 |
| MluCI | AATT | 3 cut(s) | 4, 856, 1071 |
| MlyI | GAGTC | 2 cut(s) | 563, 895 |
| MmeI | TCCRAC | 3 cut(s) | 265, 566, 933 |
| MseI | TTAA | 2 cut(s) | 723, 774 |
| MspCI | CTTAAG | 1 cut(s) | 722 |
| MspI | CCGG | 3 cut(s) | 77, 94, 136 |
| MspR9I | CCNGG | 2 cut(s) | 77, 1051 |
| MvaI | CCWGG | 1 cut(s) | 1051 |
| MvnI | CGCG | 5 cut(s) | 118, 342, 344, 412, 476 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 275, 290, 623 |
| NciI | CCSGG | 1 cut(s) | 77 |
| NdeII | GATC | 5 cut(s) | 169, 192, 374, 538, 891 |
| NlaIII | CATG | 5 cut(s) | 535, 749, 835, 1177, 1189 |
| NlaIV | GGNNCC | 2 cut(s) | 431, 462 |
| NmeAIII | GCCGAG | 1 cut(s) | 437 |
| NspI | RCATGY | 1 cut(s) | 835 |
| PagI | TCATGA | 1 cut(s) | 745 |
| PaqCI | CACCTGC | 2 cut(s) | 206, 311 |
| PauI | GCGCGC | 2 cut(s) | 340, 410 |
| PciI | ACATGT | 1 cut(s) | 831 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 253, 488 |
| PfeI | GAWTC | 3 cut(s) | 258, 972, 1030 |
| PflFI | GACNNNGTC | 1 cut(s) | 118 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 470, 777 |
| PkrI | GCNGC | 9 cut(s) | 203, 292, 316, 366, 369, 384, 406, 409, 478 |
| Ple19I | CGATCG | 1 cut(s) | 377 |
| PleI | GAGTC | 2 cut(s) | 563, 895 |
| PmaCI | CACGTG | 1 cut(s) | 1156 |
| PmlI | CACGTG | 1 cut(s) | 1156 |
| PpsI | GAGTC | 2 cut(s) | 563, 895 |
| Ppu21I | YACGTR | 1 cut(s) | 1156 |
| PscI | ACATGT | 1 cut(s) | 831 |
| Psp6I | CCWGG | 1 cut(s) | 1049 |
| PspCI | CACGTG | 1 cut(s) | 1156 |
| PspFI | CCCAGC | 3 cut(s) | 252, 326, 355 |
| PspGI | CCWGG | 1 cut(s) | 1049 |
| PspN4I | GGNNCC | 2 cut(s) | 431, 462 |
| PspPI | GGNCC | 7 cut(s) | 57, 91, 120, 429, 460, 677, 1053 |
| PstI | CTGCAG | 2 cut(s) | 203, 409 |
| PsyI | GACNNNGTC | 1 cut(s) | 118 |
| PteI | GCGCGC | 2 cut(s) | 340, 410 |
| PvuI | CGATCG | 1 cut(s) | 377 |
| RsaI | GTAC | 1 cut(s) | 587 |
| RsaNI | GTAC | 1 cut(s) | 586 |
| SaqAI | TTAA | 2 cut(s) | 723, 774 |
| SatI | GCNGC | 9 cut(s) | 202, 291, 315, 365, 368, 383, 405, 408, 477 |
| Sau3AI | GATC | 5 cut(s) | 169, 192, 374, 538, 891 |
| Sau96I | GGNCC | 7 cut(s) | 57, 91, 120, 429, 460, 677, 1053 |
| SchI | GAGTC | 2 cut(s) | 563, 895 |
| ScrFI | CCNGG | 2 cut(s) | 77, 1051 |
| SfaNI | GCATC | 1 cut(s) | 706 |
| SfcI | CTRYAG | 2 cut(s) | 199, 405 |
| SinI | GGWCC | 4 cut(s) | 57, 91, 120, 677 |
| SmlI | CTYRAG | 2 cut(s) | 581, 722 |
| SmoI | CTYRAG | 2 cut(s) | 581, 722 |
| Sse9I | AATT | 3 cut(s) | 4, 856, 1071 |
| SsiI | CCGC | 4 cut(s) | 118, 262, 476, 790 |
| SspMI | CTAG | 2 cut(s) | 452, 935 |
| StyD4I | CCNGG | 2 cut(s) | 75, 1049 |
| StyI | CCWWGG | 1 cut(s) | 425 |
| TaaI | ACNGT | 2 cut(s) | 681, 711 |
| TaiI | ACGT | 5 cut(s) | 28, 733, 853, 1138, 1158 |
| TaqI | TCGA | 4 cut(s) | 67, 172, 191, 547 |
| TasI | AATT | 3 cut(s) | 4, 856, 1071 |
| TauI | GCSGC | 1 cut(s) | 479 |
| TfiI | GAWTC | 3 cut(s) | 258, 972, 1030 |
| Tru1I | TTAA | 2 cut(s) | 723, 774 |
| Tru9I | TTAA | 2 cut(s) | 723, 774 |
| TscAI | CASTG | 4 cut(s) | 166, 475, 643, 1063 |
| TseI | GCWGC | 8 cut(s) | 201, 290, 314, 364, 367, 382, 404, 407 |
| TspDTI | ATGAA | 6 cut(s) | 17, 167, 337, 734, 798, 1112 |
| TspGWI | ACGGA | 2 cut(s) | 70, 90 |
| TspRI | CASTG | 4 cut(s) | 166, 475, 643, 1063 |
| Tth111I | GACNNNGTC | 1 cut(s) | 118 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 470, 777 |
| Vha464I | CTTAAG | 1 cut(s) | 722 |
| VpaK11BI | GGWCC | 4 cut(s) | 57, 91, 120, 677 |
| XapI | RAATTY | 3 cut(s) | 4, 856, 1071 |
| XceI | RCATGY | 1 cut(s) | 835 |
| XspI | CTAG | 2 cut(s) | 452, 935 |