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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 250, 442 |
| Acc16I | TGCGCA | 1 cut(s) | 159 |
| Acc36I | ACCTGC | 2 cut(s) | 250, 442 |
| AccII | CGCG | 8 cut(s) | 177, 204, 311, 349, 369, 396, 503, 541 |
| AclWI | GGATC | 4 cut(s) | 157, 251, 349, 443 |
| AcoI | YGGCCR | 1 cut(s) | 56 |
| AcsI | RAATTY | 1 cut(s) | 569 |
| AcuI | CTGAAG | 1 cut(s) | 746 |
| AdeI | CACNNNGTG | 1 cut(s) | 70 |
| AfaI | GTAC | 2 cut(s) | 602, 758 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 33, 54, 610, 751 |
| AflIII | ACRYGT | 1 cut(s) | 730 |
| AgsI | TTSAA | 1 cut(s) | 569 |
| AluBI | AGCT | 2 cut(s) | 334, 526 |
| AluI | AGCT | 2 cut(s) | 334, 526 |
| Alw21I | GWGCWC | 1 cut(s) | 54 |
| Alw44I | GTGCAC | 1 cut(s) | 50 |
| AlwI | GGATC | 4 cut(s) | 157, 251, 349, 443 |
| AoxI | GGCC | 7 cut(s) | 56, 270, 316, 342, 462, 508, 534 |
| ApaLI | GTGCAC | 1 cut(s) | 50 |
| ApeKI | GCWGC | 7 cut(s) | 15, 107, 136, 300, 331, 492, 523 |
| ApoI | RAATTY | 1 cut(s) | 569 |
| AspS9I | GGNCC | 2 cut(s) | 271, 463 |
| AsuC2I | CCSGG | 2 cut(s) | 341, 533 |
| AsuHPI | GGTGA | 2 cut(s) | 649, 709 |
| BaeGI | GKGCMC | 3 cut(s) | 54, 284, 476 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 584, 617 |
| BauI | CACGAG | 1 cut(s) | 149 |
| Bbv12I | GWGCWC | 1 cut(s) | 54 |
| BbvCI | CCTCAGC | 1 cut(s) | 118 |
| BbvI | GCAGC | 7 cut(s) | 27, 119, 148, 312, 343, 504, 535 |
| BccI | CCATC | 3 cut(s) | 86, 541, 584 |
| BceAI | ACGGC | 3 cut(s) | 32, 214, 406 |
| BcnI | CCSGG | 2 cut(s) | 341, 533 |
| BfaI | CTAG | 1 cut(s) | 741 |
| BfmI | CTRYAG | 4 cut(s) | 243, 298, 435, 490 |
| BfuAI | ACCTGC | 2 cut(s) | 250, 442 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 638 |
| BisI | GCNGC | 9 cut(s) | 16, 108, 137, 301, 309, 332, 493, 501, 524 |
| BlsI | GCNGC | 9 cut(s) | 17, 109, 138, 302, 310, 333, 494, 502, 525 |
| Bme1390I | CCNGG | 2 cut(s) | 341, 533 |
| BmgT120I | GGNCC | 2 cut(s) | 271, 463 |
| BmrFI | CCNGG | 2 cut(s) | 341, 533 |
| BmsI | GCATC | 3 cut(s) | 332, 524, 781 |
| Bpu10I | CCTNAGC | 1 cut(s) | 118 |
| BpuEI | CTTGAG | 1 cut(s) | 617 |
| BpuMI | CCSGG | 2 cut(s) | 341, 533 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 33, 54, 610, 751 |
| Bse1I | ACTGG | 3 cut(s) | 59, 260, 452 |
| Bse3DI | GCAATG | 2 cut(s) | 273, 465 |
| BseGI | GGATG | 3 cut(s) | 323, 515, 796 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 33, 54, 610, 751 |
| BseMI | GCAATG | 2 cut(s) | 273, 465 |
| BseMII | CTCAG | 3 cut(s) | 132, 600, 713 |
| BseNI | ACTGG | 3 cut(s) | 59, 260, 452 |
| BsePI | GCGCGC | 2 cut(s) | 175, 367 |
| BseSI | GKGCMC | 3 cut(s) | 54, 284, 476 |
| BseXI | GCAGC | 7 cut(s) | 27, 119, 148, 312, 343, 504, 535 |
| BseYI | CCCAGC | 7 cut(s) | 220, 273, 304, 412, 465, 496, 750 |
| Bsh1236I | CGCG | 8 cut(s) | 177, 204, 311, 349, 369, 396, 503, 541 |
| BshFI | GGCC | 7 cut(s) | 58, 272, 318, 344, 464, 510, 536 |
| BsiHKAI | GWGCWC | 1 cut(s) | 54 |
| BsiSI | CCGG | 4 cut(s) | 315, 341, 507, 533 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 33, 54, 610, 751 |
| BsnI | GGCC | 7 cut(s) | 58, 272, 318, 344, 464, 510, 536 |
| Bsp1286I | GDGCHC | 3 cut(s) | 54, 284, 476 |
| Bsp143I | GATC | 6 cut(s) | 162, 247, 256, 354, 439, 448 |
| BspANI | GGCC | 7 cut(s) | 58, 272, 318, 344, 464, 510, 536 |
| BspCNI | CTCAG | 3 cut(s) | 131, 599, 714 |
| BspFNI | CGCG | 8 cut(s) | 177, 204, 311, 349, 369, 396, 503, 541 |
| BspHI | TCATGA | 1 cut(s) | 822 |
| BspMAI | CTGCAG | 4 cut(s) | 247, 302, 439, 494 |
| BspMI | ACCTGC | 2 cut(s) | 250, 442 |
| BspPI | GGATC | 4 cut(s) | 157, 251, 349, 443 |
| BsrDI | GCAATG | 2 cut(s) | 273, 465 |
| BsrI | ACTGG | 3 cut(s) | 59, 260, 452 |
| BssHII | GCGCGC | 2 cut(s) | 175, 367 |
| BssMI | GATC | 6 cut(s) | 162, 247, 256, 354, 439, 448 |
| BssSI | CACGAG | 1 cut(s) | 149 |
| Bst2BI | CACGAG | 1 cut(s) | 149 |
| Bst4CI | ACNGT | 1 cut(s) | 608 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 242, 434 |
| BstC8I | GCNNGC | 3 cut(s) | 156, 177, 369 |
| BstDEI | CTNAG | 4 cut(s) | 118, 586, 722, 797 |
| BstF5I | GGATG | 3 cut(s) | 323, 515, 796 |
| BstFNI | CGCG | 8 cut(s) | 177, 204, 311, 349, 369, 396, 503, 541 |
| BstKTI | GATC | 6 cut(s) | 165, 250, 259, 357, 442, 451 |
| BstMBI | GATC | 6 cut(s) | 162, 247, 256, 354, 439, 448 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 166, 212, 221, 242, 404, 413, 434, 638 |
| BstNSI | RCATGY | 1 cut(s) | 734 |
| BstSCI | CCNGG | 2 cut(s) | 339, 531 |
| BstSFI | CTRYAG | 4 cut(s) | 243, 298, 435, 490 |
| BstSLI | GKGCMC | 3 cut(s) | 54, 284, 476 |
| BstUI | CGCG | 8 cut(s) | 177, 204, 311, 349, 369, 396, 503, 541 |
| BstV1I | GCAGC | 7 cut(s) | 27, 119, 148, 312, 343, 504, 535 |
| BstX2I | RGATCY | 1 cut(s) | 162 |
| BstYI | RGATCY | 1 cut(s) | 162 |
| BsuRI | GGCC | 7 cut(s) | 58, 272, 318, 344, 464, 510, 536 |
| BtsCI | GGATG | 3 cut(s) | 323, 515, 796 |
| BtsI | GCAGTG | 2 cut(s) | 284, 476 |
| BtsIMutI | CAGTG | 3 cut(s) | 52, 284, 476 |
| BveI | ACCTGC | 2 cut(s) | 250, 442 |
| Cac8I | GCNNGC | 3 cut(s) | 156, 177, 369 |
| CciI | TCATGA | 1 cut(s) | 822 |
| Cfr13I | GGNCC | 2 cut(s) | 271, 463 |
| CseI | GACGC | 3 cut(s) | 7, 300, 492 |
| Csp6I | GTAC | 2 cut(s) | 601, 757 |
| CviAII | CATG | 2 cut(s) | 731, 823 |
| CviQI | GTAC | 2 cut(s) | 601, 757 |
| DdeI | CTNAG | 4 cut(s) | 118, 586, 722, 797 |
| DpnI | GATC | 6 cut(s) | 164, 249, 258, 356, 441, 450 |
| DpnII | GATC | 6 cut(s) | 162, 247, 256, 354, 439, 448 |
| DraIII | CACNNNGTG | 1 cut(s) | 70 |
| EaeI | YGGCCR | 1 cut(s) | 56 |
| Eco57I | CTGAAG | 1 cut(s) | 746 |
| FaeI | CATG | 2 cut(s) | 734, 826 |
| FaiI | YATR | 8 cut(s) | 252, 337, 444, 529, 638, 732, 787, 824 |
| FatI | CATG | 2 cut(s) | 730, 822 |
| FauI | CCCGC | 8 cut(s) | 174, 209, 220, 336, 366, 401, 412, 528 |
| Fnu4HI | GCNGC | 9 cut(s) | 16, 108, 137, 301, 309, 332, 493, 501, 524 |
| FokI | GGATG | 3 cut(s) | 310, 502, 803 |
| Fsp4HI | GCNGC | 9 cut(s) | 16, 108, 137, 301, 309, 332, 493, 501, 524 |
| FspBI | CTAG | 1 cut(s) | 741 |
| FspI | TGCGCA | 1 cut(s) | 159 |
| GluI | GCNGC | 9 cut(s) | 16, 108, 137, 301, 309, 332, 493, 501, 524 |
| GsaI | CCCAGC | 7 cut(s) | 224, 277, 308, 416, 469, 500, 754 |
| HaeIII | GGCC | 7 cut(s) | 58, 272, 318, 344, 464, 510, 536 |
| HapII | CCGG | 4 cut(s) | 315, 341, 507, 533 |
| HgaI | GACGC | 3 cut(s) | 7, 300, 492 |
| Hin1II | CATG | 2 cut(s) | 734, 826 |
| HinfI | GANTC | 3 cut(s) | 73, 584, 737 |
| HpaII | CCGG | 4 cut(s) | 315, 341, 507, 533 |
| HphI | GGTGA | 2 cut(s) | 649, 709 |
| Hpy166II | GTNNAC | 1 cut(s) | 52 |
| Hpy188I | TCNGA | 2 cut(s) | 589, 723 |
| Hpy188III | TCNNGA | 1 cut(s) | 823 |
| Hpy8I | GTNNAC | 1 cut(s) | 52 |
| Hpy99I | CGWCG | 1 cut(s) | 23 |
| HpyAV | CCTTC | 3 cut(s) | 226, 418, 770 |
| HpyCH4III | ACNGT | 1 cut(s) | 608 |
| HpyCH4IV | ACGT | 1 cut(s) | 66 |
| HpyCH4V | TGCA | 8 cut(s) | 15, 52, 236, 245, 300, 428, 437, 492 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 166, 212, 221, 242, 404, 413, 434, 638 |
| HpyF3I | CTNAG | 4 cut(s) | 118, 586, 722, 797 |
| HpySE526I | ACGT | 1 cut(s) | 66 |
| Hsp92II | CATG | 2 cut(s) | 734, 826 |
| Kzo9I | GATC | 6 cut(s) | 162, 247, 256, 354, 439, 448 |
| LmnI | GCTCC | 1 cut(s) | 839 |
| Lsp1109I | GCAGC | 7 cut(s) | 27, 119, 148, 312, 343, 504, 535 |
| LweI | GCATC | 3 cut(s) | 332, 524, 781 |
| MaeI | CTAG | 1 cut(s) | 741 |
| MaeII | ACGT | 1 cut(s) | 66 |
| MalI | GATC | 6 cut(s) | 164, 249, 258, 356, 441, 450 |
| MboI | GATC | 6 cut(s) | 162, 247, 256, 354, 439, 448 |
| MflI | RGATCY | 1 cut(s) | 162 |
| MhlI | GDGCHC | 3 cut(s) | 54, 284, 476 |
| MluCI | AATT | 2 cut(s) | 569, 782 |
| MlyI | GAGTC | 1 cut(s) | 578 |
| MmeI | TCCRAC | 1 cut(s) | 581 |
| MnlI | CCTC | 9 cut(s) | 121, 127, 235, 329, 355, 427, 521, 547, 717 |
| MseI | TTAA | 4 cut(s) | 290, 482, 713, 767 |
| MspI | CCGG | 4 cut(s) | 315, 341, 507, 533 |
| MspR9I | CCNGG | 2 cut(s) | 341, 533 |
| MvnI | CGCG | 8 cut(s) | 177, 204, 311, 349, 369, 396, 503, 541 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 166, 212, 221, 242, 404, 413, 434, 638 |
| NciI | CCSGG | 2 cut(s) | 341, 533 |
| NdeII | GATC | 6 cut(s) | 162, 247, 256, 354, 439, 448 |
| NlaIII | CATG | 2 cut(s) | 734, 826 |
| NmeAIII | GCCGAG | 1 cut(s) | 13 |
| NsbI | TGCGCA | 1 cut(s) | 159 |
| NspI | RCATGY | 1 cut(s) | 734 |
| PagI | TCATGA | 1 cut(s) | 822 |
| PaqCI | CACCTGC | 2 cut(s) | 250, 442 |
| PauI | GCGCGC | 2 cut(s) | 175, 367 |
| PciI | ACATGT | 1 cut(s) | 730 |
| PfeI | GAWTC | 2 cut(s) | 73, 737 |
| PkrI | GCNGC | 9 cut(s) | 17, 109, 138, 302, 310, 333, 494, 502, 525 |
| PleI | GAGTC | 1 cut(s) | 578 |
| PpsI | GAGTC | 1 cut(s) | 578 |
| PscI | ACATGT | 1 cut(s) | 730 |
| PspFI | CCCAGC | 7 cut(s) | 220, 273, 304, 412, 465, 496, 750 |
| PspPI | GGNCC | 2 cut(s) | 271, 463 |
| PstI | CTGCAG | 4 cut(s) | 247, 302, 439, 494 |
| PsuI | RGATCY | 1 cut(s) | 162 |
| PteI | GCGCGC | 2 cut(s) | 175, 367 |
| RsaI | GTAC | 2 cut(s) | 602, 758 |
| RsaNI | GTAC | 2 cut(s) | 601, 757 |
| SaqAI | TTAA | 4 cut(s) | 290, 482, 713, 767 |
| SatI | GCNGC | 9 cut(s) | 16, 108, 137, 301, 309, 332, 493, 501, 524 |
| Sau3AI | GATC | 6 cut(s) | 162, 247, 256, 354, 439, 448 |
| Sau96I | GGNCC | 2 cut(s) | 271, 463 |
| SchI | GAGTC | 1 cut(s) | 578 |
| ScrFI | CCNGG | 2 cut(s) | 341, 533 |
| SduI | GDGCHC | 3 cut(s) | 54, 284, 476 |
| SetI | ASST | 9 cut(s) | 69, 119, 244, 336, 436, 528, 699, 762, 832 |
| SfaNI | GCATC | 3 cut(s) | 332, 524, 781 |
| SfcI | CTRYAG | 4 cut(s) | 243, 298, 435, 490 |
| SmlI | CTYRAG | 1 cut(s) | 596 |
| SmoI | CTYRAG | 1 cut(s) | 596 |
| Sse9I | AATT | 2 cut(s) | 569, 782 |
| SspI | AATATT | 1 cut(s) | 11 |
| SspMI | CTAG | 1 cut(s) | 741 |
| StyD4I | CCNGG | 2 cut(s) | 339, 531 |
| TaaI | ACNGT | 1 cut(s) | 608 |
| TaiI | ACGT | 1 cut(s) | 69 |
| TaqI | TCGA | 2 cut(s) | 113, 562 |
| TasI | AATT | 2 cut(s) | 569, 782 |
| TauI | GCSGC | 2 cut(s) | 311, 503 |
| TfiI | GAWTC | 2 cut(s) | 73, 737 |
| Tru1I | TTAA | 4 cut(s) | 290, 482, 713, 767 |
| Tru9I | TTAA | 4 cut(s) | 290, 482, 713, 767 |
| TscAI | CASTG | 3 cut(s) | 59, 284, 476 |
| TseI | GCWGC | 7 cut(s) | 15, 107, 136, 300, 331, 492, 523 |
| TspDTI | ATGAA | 2 cut(s) | 696, 811 |
| TspGWI | ACGGA | 1 cut(s) | 607 |
| TspRI | CASTG | 3 cut(s) | 59, 284, 476 |
| VneI | GTGCAC | 1 cut(s) | 50 |
| XapI | RAATTY | 1 cut(s) | 569 |
| XceI | RCATGY | 1 cut(s) | 734 |
| XspI | CTAG | 1 cut(s) | 741 |