MD10G1255900.v1.1

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000158
Physical Location & Seq
Reverse (-)
35044365 .. 35049082
4718 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1255900.v1.1.491

Sequence Viewer

Length: 1740 bp
ATGGCTTGGCGCAACATCTGCAAGAACCAGCCTCTCATGTGTCGCACCATCGACATGCACAATGACGGTGATCTCGATGAGATGCCGTACAACTTGGAGAAGATGTGCCGCCTTGCCGTTGATCGCAGCTGCGGTACTCTGGTCTATATTAATGTCGAACACTTCGGCACCGATGATCTCCTTAAGGATATCACTGATAAGTATTTTATTAATTTTCGTTGCTTGTTAAATGTTTGGTTGGATGATAAAGGCACACTCACTGATGACGTTAATTCTGCATTGGATGCATGGATTCAGCAAGATGCCATGGTGCTTTTGTGGATCAACAGTTCCGCTCATCCTACTGTTCTTGCTTCTCTCATTGGTAAGACAAGTTCATATTCTGCATCGACTTGCTTACGTGAACAATATGCTTCTCAATCTACTGGTCGTCTACTACAACTACGTAGTGAGCTTATGAGTACCCATCGTGGTGATTCACCTATTGCTGATTTTCTTGACCACGTCAAAAGCTCCATACTGTGTTCGAAAATGGACCCACTGCTCTTGCTGTTGGACGGGGCCGTTCCGGTCCTCATCGTGGTGGTGGCGCTACTCGTGGTGGTCGTGGTCCCTTCCATAGTGGTCGTGGTGGTTAGTTTGCTCTTCGGCCCCCCGGCCAAGGCCAACATGCTCCTCACCCTCGCCCTGCTCATGATGGTTTGTCGTGGCATCATATGCTTGCGACTCATGTGTTGCTGGGACTTATCAGATGAAGGATTGAGTAAAGTGGCTCCAAATCTTCCTCTGTTAGAGGAGCTTGAGGTTTCATTAAGCAGACTCTCAAGGGATTCAATGGAAGTGGTCGGGGGCTTTCCAGCTTTTGAAGTCATTCAAAATGAATGCCCAGTGGTCAGGCCCGACCTTAAGAATTTGGGAGCCCTAGGCGAGTCTTCAAATTGGGGCCCTAAAGTTCTCGGACCTCCGACTCTTTGTATATTGGTGTTTCAAACTGCCCACCCCAATACAAACAGAGTGAAACCGTTTCCACTGCACCAACGAGCTAATTGTGATGTTTCTTACATTTTCTTTATTTATATTTTATATACAGGATGTATAAAAAAAATTGGCCCCCCCACCCCGAACTTGGGGGGCGCTTTGGGCTTACCATTCCCTCACGATTTAGTTTTTAGGAACTCACGAGCAACTTCCCAGTGGGTTACCCATCCTGGGAGTGCTCTGGCCTCCTTCTCGCTTAACTTTGGAGTTCCTACAGAATCCGAAGCTAGTGAGCTCCCAAAAAGCCTCGTGCTAGGTAGGGATGAGAATATACATTTAAGGATCACTCCCCTGGGCGATATGGGATGTTACAGAGACGATGAGTTGAATAAGTTTGACAATGTTCCTTGTCACATAACAGATTGCGATTCGGTTGCTAGCGCTGGAGGGATGCATGATTTGCACCACCTCCAACTTTTCGGGAATCAACTGACTAATGAGGGATTGAAAGAAATTGTTGATTGTTGTCCACATCTTGAGTCGCTGGATTTGCGCCATTGTTTGAATCTTGATCTGAGCGGAGATTTGGGAAAAAGATGTGTTGAACGCATTCAAAATTTGCTGCTCCCAAATGATTCCATTGACGACTATGAATTTCTTGCTACATCATATTCTTATGGACCACATGGTTATGATATGGGAGCATATGGGTTTTCTGGTGATTACTGGTATGATGACTATGATTGCTATGATGGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

580

Amino Acids

64.18

Weight (kDa)

4.96

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000158)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10163
malus_domestica MD04G1068000.v1.1 MD05G1130900.v1.1 MD06G1153600.v1.1 MD10G1255900.v1.1 MD10G1282000.v1.1 MD13G1075400.v1.1 MD13G1192400.v1.1
pyrus_communis pycom01g00130 pycom01g00390 pycom01g00700 pycom01g00720 pycom01g00750 pycom01g00850 pycom01g00870 pycom01g00910 pycom01g01250 pycom01g01560 pycom01g01680 pycom01g01860 pycom01g01900 pycom01g01910 pycom01g02200 pycom01g02260 pycom01g02270 pycom01g02280 pycom01g02330 pycom01g02480 pycom01g02720 pycom01g02730 pycom01g02750 pycom01g02770 pycom01g02780 pycom01g02860 pycom01g02910 pycom01g03540 pycom01g04090 pycom01g04100 pycom01g04150 pycom01g04250 pycom01g04430 pycom01g11810 pycom01g11860 pycom01g11940 pycom01g21380 pycom02g06650 pycom02g08490 pycom02g08600 pycom02g18230 pycom02g18450 pycom02g18800 pycom02g19150 pycom02g20740 pycom02g21340 pycom02g26210 pycom03g12060 pycom03g19060 pycom03g19100 pycom03g19120 pycom04g06890 pycom04g08960 pycom05g01790 pycom05g07910 pycom05g08080 pycom05g08100 pycom05g08570 pycom05g21500 pycom05g22400 pycom05g22510 pycom05g22620 pycom05g23020 pycom05g23720 pycom05g23890 pycom05g24040 pycom07g12080 pycom08g07930 pycom08g11940 pycom09g12120 pycom10g03890 pycom10g23360 pycom10g28250 pycom11g02850 pycom11g14370 pycom11g14400 pycom11g21840 pycom11g21860 pycom11g21980 pycom11g23580 pycom11g23650 pycom11g24050 pycom11g25610 pycom12424g00060 pycom1256g00130 pycom12661g00030 pycom12g09520 pycom12g09530 pycom12g09660 pycom12g09730 pycom12g11210 pycom12g16710 pycom12g16740 pycom12g16750 pycom13g08800 pycom13g18580 pycom13g18670 pycom13g18770 pycom13g22900 pycom13g23050 pycom13g23160 pycom13g23480 pycom13g23610 pycom13g23660 pycom13g23790 pycom13g23840 pycom13g24010 pycom13g24140 pycom13g24210 pycom13g24300 pycom13g24310 pycom13g24480 pycom13g24520 pycom13g24570 pycom13g24730 pycom13g24760 pycom13g24820 pycom13g25010 pycom13g25020 pycom13g25270 pycom13g25620 pycom13g25670 pycom13g25760 pycom13g25800 pycom13g25900 pycom13g26620 pycom13g26640 pycom13g26670 pycom13g26940 pycom13g27040 pycom13g27130 pycom13g27530 pycom13g27770 pycom13g27830 pycom13g27920 pycom13g27940 pycom13g28100 pycom13g28420 pycom13g28430 pycom13g28460 pycom13g28520 pycom13g29160 pycom14g05380 pycom15g03480 pycom15g04370 pycom15g27500 pycom16g13590 pycom16g13610 pycom16g13630 pycom16g14390 pycom16g18310 pycom17g01940 pycom17g04000 pycom17g16100 pycom17g16220 pycom17g16230 pycom17g16340 pycom17g16430 pycom17g16460 pycom17g16740 pycom17g16960 pycom17g17020 pycom17g17090 pycom17g17100 pycom17g17170 pycom17g17430 pycom17g17850 pycom17g18230 pycom17g18250 pycom17g18380 pycom17g21170 pycom17g21330 pycom520g00150 pycom520g00160 pycom808g00030 pycom808g00160 pycom808g00250 pycom808g00290
rosa_chinensis RchiOBHm_Chr5g0054451

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 167
AccBSI CCGCTC 2 cut(s) 335, 1557
AccI GTMKAC 1 cut(s) 433
AciI CCGC 4 cut(s) 109, 132, 333, 1557
AclWI GGATC 2 cut(s) 329, 1328
AcoI YGGCCR 1 cut(s) 657
AcsI RAATTY 3 cut(s) 910, 1594, 1631
AfaI GTAC 3 cut(s) 89, 136, 463
AfeI AGCGCT 1 cut(s) 1420
AfiI CCNNNNNNNGG 5 cut(s) 580, 661, 1126, 1127, 1209
AflII CTTAAG 2 cut(s) 182, 905
AjiI CACGTC 1 cut(s) 505
AjnI CCWGG 2 cut(s) 1207, 1329
AloI GAACNNNNNNTCC 2 cut(s) 313, 345
AluBI AGCT 8 cut(s) 129, 454, 513, 799, 860, 1043, 1265, 1273
AluI AGCT 8 cut(s) 129, 454, 513, 799, 860, 1043, 1265, 1273
Alw21I GWGCWC 2 cut(s) 1219, 1275
Alw26I GTCTC 1 cut(s) 1347
AlwI GGATC 2 cut(s) 329, 1328
Aor51HI AGCGCT 1 cut(s) 1420
AoxI GGCC 8 cut(s) 561, 649, 657, 663, 896, 943, 1108, 1221
ApaI GGGCCC 1 cut(s) 947
ApeKI GCWGC 3 cut(s) 126, 129, 1600
ApoI RAATTY 3 cut(s) 910, 1594, 1631
AseI ATTAAT 2 cut(s) 150, 210
Asp700I GAANNNNTTC 2 cut(s) 870, 1587
AspA2I CCTAGG 1 cut(s) 922
AspLEI GCGC 5 cut(s) 12, 592, 1136, 1421, 1533
AsuC2I CCSGG 1 cut(s) 656
AsuHPI GGTGA 5 cut(s) 80, 471, 485, 670, 1709
AsuII TTCGAA 1 cut(s) 527
AsuNHI GCTAGC 1 cut(s) 1415
AvaII GGWCC 5 cut(s) 535, 571, 610, 959, 1658
AvrII CCTAGG 1 cut(s) 922
BaeGI GKGCMC 1 cut(s) 947
BaeI ACNNNNGTAYC 2 cut(s) 126, 159
BanI GGYRCC 1 cut(s) 167
BanII GRGCYC 3 cut(s) 922, 947, 1275
BauI CACGAG 3 cut(s) 596, 1179, 1286
BbsI GAAGAC 1 cut(s) 924
Bbv12I GWGCWC 2 cut(s) 1219, 1275
BbvI GCAGC 3 cut(s) 116, 138, 1587
BccI CCATC 5 cut(s) 56, 474, 691, 1212, 1724
BceAI ACGGC 3 cut(s) 70, 101, 548
BcgI CGANNNNNNTGC 2 cut(s) 1394, 1428
BciT130I CCWGG 2 cut(s) 1209, 1331
BcnI CCSGG 1 cut(s) 656
BcoDI GTCTC 1 cut(s) 1347
BfaI CTAG 4 cut(s) 923, 1266, 1292, 1416
BfmI CTRYAG 1 cut(s) 1251
BfoI RGCGCY 3 cut(s) 593, 1137, 1422
BfrI CTTAAG 2 cut(s) 182, 905
BisI GCNGC 4 cut(s) 109, 127, 130, 1601
BlnI CCTAGG 1 cut(s) 922
BlsI GCNGC 4 cut(s) 110, 128, 131, 1602
Bme1390I CCNGG 3 cut(s) 656, 1209, 1331
Bme18I GGWCC 5 cut(s) 535, 571, 610, 959, 1658
BmgBI CACGTC 1 cut(s) 505
BmrFI CCNGG 3 cut(s) 656, 1209, 1331
BmrI ACTGGG 2 cut(s) 881, 1186
BmsI GCATC 6 cut(s) 72, 274, 292, 395, 720, 1419
BmtI GCTAGC 1 cut(s) 1419
BmuI ACTGGG 2 cut(s) 881, 1186
BpiI GAAGAC 1 cut(s) 924
BpmI CTGGAG 1 cut(s) 1443
Bpu14I TTCGAA 1 cut(s) 527
BpuEI CTTGAG 3 cut(s) 808, 821, 1535
BpuMI CCSGG 1 cut(s) 656
BsaAI YACGTR 2 cut(s) 401, 446
BsaJI CCNNGG 7 cut(s) 306, 654, 660, 922, 1208, 1329, 1330
BsaWI WCCGGW 1 cut(s) 568
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 5 cut(s) 580, 661, 1126, 1127, 1209
Bse1I ACTGG 4 cut(s) 430, 887, 1192, 1709
BseBI CCWGG 2 cut(s) 1209, 1331
BseDI CCNNGG 7 cut(s) 306, 654, 660, 922, 1208, 1329, 1330
BseGI GGATG 8 cut(s) 247, 289, 337, 1097, 1204, 1306, 1349, 1434
BseLI CCNNNNNNNGG 5 cut(s) 580, 661, 1126, 1127, 1209
BseMII CTCAG 1 cut(s) 1544
BseNI ACTGG 4 cut(s) 430, 887, 1192, 1709
BseRI GAGGAG 2 cut(s) 665, 809
BseSI GKGCMC 1 cut(s) 947
BseXI GCAGC 3 cut(s) 116, 138, 1587
BseYI CCCAGC 1 cut(s) 738
BsgI GTGCAG 1 cut(s) 1016
BshFI GGCC 8 cut(s) 563, 651, 659, 665, 898, 945, 1110, 1223
BshNI GGYRCC 1 cut(s) 167
BsiHKAI GWGCWC 2 cut(s) 1219, 1275
BsiSI CCGG 2 cut(s) 569, 656
BslFI GGGAC 2 cut(s) 596, 755
BslI CCNNNNNNNGG 5 cut(s) 580, 661, 1126, 1127, 1209
BsmAI GTCTC 1 cut(s) 1347
BsmBI CGTCTC 1 cut(s) 1347
BsmFI GGGAC 2 cut(s) 596, 755
BsmI GAATGC 2 cut(s) 887, 1587
BsnI GGCC 8 cut(s) 563, 651, 659, 665, 898, 945, 1110, 1223
Bsp119I TTCGAA 1 cut(s) 527
Bsp120I GGGCCC 1 cut(s) 943
Bsp1286I GDGCHC 4 cut(s) 922, 947, 1219, 1275
Bsp143I GATC 6 cut(s) 70, 121, 175, 321, 1320, 1549
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 4 cut(s) 109, 132, 333, 1557
BspANI GGCC 8 cut(s) 563, 651, 659, 665, 898, 945, 1110, 1223
BspCNI CTCAG 1 cut(s) 1545
BspHI TCATGA 1 cut(s) 693
BspOI GCTAGC 1 cut(s) 1419
BspPI GGATC 2 cut(s) 329, 1328
BspQI GCTCTTC 1 cut(s) 650
BspT104I TTCGAA 1 cut(s) 527
BspT107I GGYRCC 1 cut(s) 167
BspTI CTTAAG 2 cut(s) 182, 905
BsrBI CCGCTC 2 cut(s) 335, 1557
BsrI ACTGG 4 cut(s) 430, 887, 1192, 1709
BssECI CCNNGG 7 cut(s) 306, 654, 660, 922, 1208, 1329, 1330
BssMI GATC 6 cut(s) 70, 121, 175, 321, 1320, 1549
BssSI CACGAG 3 cut(s) 596, 1179, 1286
BssT1I CCWWGG 3 cut(s) 306, 660, 922
Bst2BI CACGAG 3 cut(s) 596, 1179, 1286
Bst2UI CCWGG 2 cut(s) 1209, 1331
Bst4CI ACNGT 5 cut(s) 68, 329, 346, 522, 1023
Bst6I CTCTTC 1 cut(s) 650
BstAFI CTTAAG 2 cut(s) 182, 905
BstAPI GCANNNNNTGC 4 cut(s) 18, 284, 717, 1438
BstBAI YACGTR 2 cut(s) 401, 446
BstBI TTCGAA 1 cut(s) 527
BstC8I GCNNGC 2 cut(s) 722, 1417
BstDEI CTNAG 1 cut(s) 1553
BstDSI CCRYGG 1 cut(s) 306
BstEII GGTNACC 1 cut(s) 1198
BstF5I GGATG 8 cut(s) 247, 289, 337, 1097, 1204, 1306, 1349, 1434
BstH2I RGCGCY 3 cut(s) 593, 1137, 1422
BstHHI GCGC 5 cut(s) 12, 592, 1136, 1421, 1533
BstKTI GATC 6 cut(s) 73, 124, 178, 324, 1323, 1552
BstMAI GTCTC 1 cut(s) 1347
BstMBI GATC 6 cut(s) 70, 121, 175, 321, 1320, 1549
BstMWI GCNNNNNNNGC 6 cut(s) 18, 284, 717, 1140, 1438, 1528
BstNI CCWGG 2 cut(s) 1209, 1331
BstNSI RCATGY 2 cut(s) 58, 673
BstPI GGTNACC 1 cut(s) 1198
BstSCI CCNGG 3 cut(s) 654, 1207, 1329
BstSFI CTRYAG 1 cut(s) 1251
BstSLI GKGCMC 1 cut(s) 947
BstSNI TACGTA 1 cut(s) 446
BstV1I GCAGC 3 cut(s) 116, 138, 1587
BstV2I GAAGAC 1 cut(s) 924
BsuRI GGCC 8 cut(s) 563, 651, 659, 665, 898, 945, 1110, 1223
BtgI CCRYGG 1 cut(s) 306
BtrI CACGTC 1 cut(s) 505
BtsCI GGATG 8 cut(s) 247, 289, 337, 1097, 1204, 1306, 1349, 1434
BtsI GCAGTG 2 cut(s) 539, 1028
BtsIMutI CAGTG 6 cut(s) 192, 258, 539, 894, 1028, 1199
Cac8I GCNNGC 2 cut(s) 722, 1417
CciI TCATGA 1 cut(s) 693
CfoI GCGC 5 cut(s) 12, 592, 1136, 1421, 1533
Csp6I GTAC 3 cut(s) 88, 135, 462
CspCI CAANNNNNGTGG 4 cut(s) 528, 563, 822, 857
CviAII CATG 9 cut(s) 37, 55, 288, 307, 670, 694, 730, 1433, 1664
CviQI GTAC 3 cut(s) 88, 135, 462
DdeI CTNAG 1 cut(s) 1553
DpnI GATC 6 cut(s) 72, 123, 177, 323, 1322, 1551
DpnII GATC 6 cut(s) 70, 121, 175, 321, 1320, 1549
EaeI YGGCCR 1 cut(s) 657
Eam1104I CTCTTC 1 cut(s) 650
EarI CTCTTC 1 cut(s) 650
Ecl136II GAGCTC 1 cut(s) 1273
Eco105I TACGTA 1 cut(s) 446
Eco130I CCWWGG 3 cut(s) 306, 660, 922
Eco24I GRGCYC 3 cut(s) 922, 947, 1275
Eco32I GATATC 1 cut(s) 190
Eco47I GGWCC 5 cut(s) 535, 571, 610, 959, 1658
Eco47III AGCGCT 1 cut(s) 1420
Eco53kI GAGCTC 1 cut(s) 1273
Eco91I GGTNACC 1 cut(s) 1198
EcoICRI GAGCTC 1 cut(s) 1273
EcoO109I RGGNCCY 2 cut(s) 943, 944
EcoO65I GGTNACC 1 cut(s) 1198
EcoRII CCWGG 2 cut(s) 1207, 1329
EcoRV GATATC 1 cut(s) 190
EcoT14I CCWWGG 3 cut(s) 306, 660, 922
EcoT22I ATGCAT 2 cut(s) 289, 1434
EcoT38I GRGCYC 3 cut(s) 922, 947, 1275
ErhI CCWWGG 3 cut(s) 306, 660, 922
Esp3I CGTCTC 1 cut(s) 1347
FaeI CATG 9 cut(s) 40, 58, 291, 310, 673, 697, 733, 1436, 1667
FaqI GGGAC 2 cut(s) 596, 755
FatI CATG 9 cut(s) 36, 54, 287, 306, 669, 693, 729, 1432, 1663
FauNDI CATATG 2 cut(s) 716, 1684
FblI GTMKAC 1 cut(s) 433
Fnu4HI GCNGC 4 cut(s) 109, 127, 130, 1601
FokI GGATG 8 cut(s) 254, 296, 324, 1104, 1191, 1313, 1356, 1441
FriOI GRGCYC 3 cut(s) 922, 947, 1275
Fsp4HI GCNGC 4 cut(s) 109, 127, 130, 1601
FspBI CTAG 4 cut(s) 923, 1266, 1292, 1416
GlaI GCGC 5 cut(s) 11, 591, 1135, 1420, 1532
GluI GCNGC 4 cut(s) 109, 127, 130, 1601
GsaI CCCAGC 1 cut(s) 742
GsuI CTGGAG 1 cut(s) 1443
HaeII RGCGCY 3 cut(s) 593, 1137, 1422
HaeIII GGCC 8 cut(s) 563, 651, 659, 665, 898, 945, 1110, 1223
HapII CCGG 2 cut(s) 569, 656
HhaI GCGC 5 cut(s) 12, 592, 1136, 1421, 1533
Hin1II CATG 9 cut(s) 40, 58, 291, 310, 673, 697, 733, 1436, 1667
Hin6I GCGC 5 cut(s) 10, 590, 1134, 1419, 1531
HinP1I GCGC 5 cut(s) 10, 590, 1134, 1419, 1531
HpaII CCGG 2 cut(s) 569, 656
HphI GGTGA 5 cut(s) 80, 471, 485, 670, 1709
Hpy166II GTNNAC 3 cut(s) 404, 434, 1508
Hpy188I TCNGA 5 cut(s) 751, 959, 966, 1261, 1554
Hpy188III TCNNGA 8 cut(s) 74, 497, 694, 1157, 1179, 1459, 1514, 1547
Hpy8I GTNNAC 3 cut(s) 404, 434, 1508
HpyAV CCTTC 3 cut(s) 624, 749, 1237
HpyCH4III ACNGT 5 cut(s) 68, 329, 346, 522, 1023
HpyCH4IV ACGT 4 cut(s) 267, 400, 445, 504
HpyCH4V TGCA 8 cut(s) 21, 58, 278, 287, 386, 1033, 1432, 1441
HpyF10VI GCNNNNNNNGC 6 cut(s) 18, 284, 717, 1140, 1438, 1528
HpyF3I CTNAG 1 cut(s) 1553
HpySE526I ACGT 4 cut(s) 267, 400, 445, 504
Hsp92II CATG 9 cut(s) 40, 58, 291, 310, 673, 697, 733, 1436, 1667
HspAI GCGC 5 cut(s) 10, 590, 1134, 1419, 1531
Kzo9I GATC 6 cut(s) 70, 121, 175, 321, 1320, 1549
LguI GCTCTTC 1 cut(s) 650
LmnI GCTCC 8 cut(s) 518, 678, 778, 796, 917, 1278, 1608, 1679
Lsp1109I GCAGC 3 cut(s) 116, 138, 1587
LweI GCATC 6 cut(s) 72, 274, 292, 395, 720, 1419
MaeI CTAG 4 cut(s) 923, 1266, 1292, 1416
MaeII ACGT 4 cut(s) 267, 400, 445, 504
MaeIII GTNAC 3 cut(s) 1198, 1346, 1388
MalI GATC 6 cut(s) 72, 123, 177, 323, 1322, 1551
MbiI CCGCTC 2 cut(s) 335, 1557
MboI GATC 6 cut(s) 70, 121, 175, 321, 1320, 1549
MboII GAAGA 4 cut(s) 112, 637, 773, 924
MhlI GDGCHC 4 cut(s) 922, 947, 1219, 1275
MluCI AATT 9 cut(s) 211, 271, 910, 937, 1045, 1104, 1491, 1594, 1631
MlyI GAGTC 5 cut(s) 720, 813, 938, 961, 1526
MmeI TCCRAC 4 cut(s) 219, 534, 989, 1474
Mph1103I ATGCAT 2 cut(s) 289, 1434
MroXI GAANNNNTTC 2 cut(s) 870, 1587
MseI TTAA 9 cut(s) 150, 183, 210, 227, 270, 812, 906, 1236, 1316
MslI CAYNNNNRTG 4 cut(s) 53, 471, 581, 1668
MspA1I CMGCKG 1 cut(s) 129
MspCI CTTAAG 2 cut(s) 182, 905
MspI CCGG 2 cut(s) 569, 656
MspR9I CCNGG 3 cut(s) 656, 1209, 1331
Mva1269I GAATGC 2 cut(s) 887, 1587
MvaI CCWGG 2 cut(s) 1209, 1331
MwoI GCNNNNNNNGC 6 cut(s) 18, 284, 717, 1140, 1438, 1528
NciI CCSGG 1 cut(s) 656
NcoI CCATGG 1 cut(s) 306
NdeI CATATG 2 cut(s) 716, 1684
NdeII GATC 6 cut(s) 70, 121, 175, 321, 1320, 1549
NheI GCTAGC 1 cut(s) 1415
NlaIII CATG 9 cut(s) 40, 58, 291, 310, 673, 697, 733, 1436, 1667
NmuCI GTSAC 1 cut(s) 1388
NsiI ATGCAT 2 cut(s) 289, 1434
NspI RCATGY 2 cut(s) 58, 673
NspV TTCGAA 1 cut(s) 527
PagI TCATGA 1 cut(s) 693
PasI CCCWGGG 1 cut(s) 1330
PciSI GCTCTTC 1 cut(s) 650
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 603
PctI GAATGC 2 cut(s) 887, 1587
PdmI GAANNNNTTC 2 cut(s) 870, 1587
PfeI GAWTC 8 cut(s) 292, 476, 830, 1256, 1406, 1462, 1543, 1613
PflFI GACNNNGTC 1 cut(s) 503
PkrI GCNGC 4 cut(s) 110, 128, 131, 1602
PleI GAGTC 5 cut(s) 720, 813, 937, 961, 1525
PpsI GAGTC 5 cut(s) 720, 813, 937, 961, 1525
Ppu21I YACGTR 2 cut(s) 401, 446
PshBI ATTAAT 2 cut(s) 150, 210
Psp124BI GAGCTC 1 cut(s) 1275
Psp6I CCWGG 2 cut(s) 1207, 1329
PspEI GGTNACC 1 cut(s) 1198
PspFI CCCAGC 1 cut(s) 738
PspGI CCWGG 2 cut(s) 1207, 1329
PspOMI GGGCCC 1 cut(s) 943
PsrI GAACNNNNNNTAC 2 cut(s) 358, 390
PsyI GACNNNGTC 1 cut(s) 503
PvuII CAGCTG 1 cut(s) 129
RsaI GTAC 3 cut(s) 89, 136, 463
RsaNI GTAC 3 cut(s) 88, 135, 462
RseI CAYNNNNRTG 4 cut(s) 53, 471, 581, 1668
SacI GAGCTC 1 cut(s) 1275
SapI GCTCTTC 1 cut(s) 650
SaqAI TTAA 9 cut(s) 150, 183, 210, 227, 270, 812, 906, 1236, 1316
SatI GCNGC 4 cut(s) 109, 127, 130, 1601
Sau3AI GATC 6 cut(s) 70, 121, 175, 321, 1320, 1549
SchI GAGTC 5 cut(s) 720, 813, 938, 961, 1526
ScrFI CCNGG 3 cut(s) 656, 1209, 1331
SduI GDGCHC 4 cut(s) 922, 947, 1219, 1275
SfaNI GCATC 6 cut(s) 72, 274, 292, 395, 720, 1419
SfcI CTRYAG 1 cut(s) 1251
SfuI TTCGAA 1 cut(s) 527
SinI GGWCC 5 cut(s) 535, 571, 610, 959, 1658
SmiMI CAYNNNNRTG 4 cut(s) 53, 471, 581, 1668
SmlI CTYRAG 5 cut(s) 182, 800, 823, 905, 1514
SmoI CTYRAG 5 cut(s) 182, 800, 823, 905, 1514
SnaBI TACGTA 1 cut(s) 446
Sse9I AATT 9 cut(s) 211, 271, 910, 937, 1045, 1104, 1491, 1594, 1631
SsiI CCGC 4 cut(s) 109, 132, 333, 1557
SspMI CTAG 4 cut(s) 923, 1266, 1292, 1416
SstI GAGCTC 1 cut(s) 1275
StyD4I CCNGG 3 cut(s) 654, 1207, 1329
StyI CCWWGG 3 cut(s) 306, 660, 922
TaaI ACNGT 5 cut(s) 68, 329, 346, 522, 1023
TaiI ACGT 4 cut(s) 270, 403, 448, 507
TaqI TCGA 5 cut(s) 51, 75, 156, 389, 527
TasI AATT 9 cut(s) 211, 271, 910, 937, 1045, 1104, 1491, 1594, 1631
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 8 cut(s) 292, 476, 830, 1256, 1406, 1462, 1543, 1613
Tru1I TTAA 9 cut(s) 150, 183, 210, 227, 270, 812, 906, 1236, 1316
Tru9I TTAA 9 cut(s) 150, 183, 210, 227, 270, 812, 906, 1236, 1316
TscAI CASTG 6 cut(s) 199, 265, 546, 894, 1035, 1199
TseFI GTSAC 1 cut(s) 1388
TseI GCWGC 3 cut(s) 126, 129, 1600
Tsp45I GTSAC 1 cut(s) 1388
TspDTI ATGAA 5 cut(s) 366, 768, 798, 894, 1644
TspRI CASTG 6 cut(s) 199, 265, 546, 894, 1035, 1199
Tth111I GACNNNGTC 1 cut(s) 503
Vha464I CTTAAG 2 cut(s) 182, 905
VpaK11BI GGWCC 5 cut(s) 535, 571, 610, 959, 1658
VspI ATTAAT 2 cut(s) 150, 210
XapI RAATTY 3 cut(s) 910, 1594, 1631
XceI RCATGY 2 cut(s) 58, 673
XcmI CCANNNNNNNNNTGG 2 cut(s) 625, 1123
XmaJI CCTAGG 1 cut(s) 922
XmiI GTMKAC 1 cut(s) 433
XmnI GAANNNNTTC 2 cut(s) 870, 1587
XspI CTAG 4 cut(s) 923, 1266, 1292, 1416
Zsp2I ATGCAT 2 cut(s) 289, 1434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.