pycom13g27040

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000158
Physical Location & Seq
Forward (+)
23473796 .. 23477090
3295 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g27040.17

Sequence Viewer

Length: 2448 bp
ATGGCTGCTACCGTTGGCATAGCCACGATGGTGCCCCATGGGGGTGGTGCTGCTCCAACTTGTTGTCGCGTTGAGCGTCAAGTTCAGTTCGTTGTGGTTTTCAAATTCGTAGTCATTGTACTTTTCTTTTACATTTATTCAAAGAACTTTATAAAAAAAAAAATTCTAGGATTAAGAACTTATGAAAAATCTACAAATGAACGAGAAATGAGAAACTTTGAATGCGCGAGTCTTCTTCGAGCATGTGAATCAAGACTCTCAATGAAAGCACCAATTTGTGAATGCAAATTTCTGCCATCTTCTCCTTTGACGAAAATGCTCCTTGGGGAGGGGGGCTTTGGCCGAAGAATCTCCGATGCCAAAGTTAAAATTTTGATTGAGCCGTTATTATCCGTTGCGTGCACGTGGGAATCTTGGTGTGCCTCGAGGGTAATTGTCCTTTTTTATGTTAGTCCCTTGTTGAGTAGTGTATTTATAGGCTTCAATGAGAGAAAACATTCATGGAGCTCTTATGACTTTTCATCGATGTTCTTCATTATTCTTCCTTTATTCGTCATGTTCATAACAAGAACAAATTGTTGGTTTTTTTTTATTCAAAGTACTAAGTTGTTTTGTTTAGTGTATGTTTGTCTTAATCTGTGTGATTTGCTTTTGTACTTTCCACTCACAAGGCTTTCCTCACACACTTTGAATTTCAAATTTTCAAAAGTCAAGCCACCCATTCCCACTACCAAAATCGGGCACCCTAAAGTGAGGGTGATTTTGGGGCTTTTTTTTTTTTACCTAAAGACGCAAATTTACCAAAGAATAAATTGTTTTGCTCCTTTAATGAAGTTTTTGTTATTGTTAATTTATTTTTATTCTAGTTGTAATCACATGAATTTACATATTCATTATGTTATATGTATTATGTGGAACTATTTAAGTTTCGAGATTGATGGGATATTAGGATTTGAGGATGAAATCTATCTGAAGTTGGTATTTCATTTAAAAAATTTGTTTATTCATCATGAATTACTAAAATATGTTACAAACCTTCTGGAAAACATTAATGTTGGAGGCGACCAAAGATCCCAAATTCAGCATACAAACGACCATGGAAGCGCACGGTGCTTATTGCTTAAGTTTCTAATTTTGGAGCCTTTGTTATCGTACAAAAAATCAAGAACCCCAACTATAAGGGAAAGTGGAAGACTCCATGGATTGATAATGTCACATCCCAGCCCAGTGTCCGTTTTGGGCCCTGACCACACCCTCACGGTTTTGTTTCTGAGAACTCACATGAGAACTTCCCAGTGGGTTACCCATCCTAGGATTGCTCTCGCGCGAACTCGCTTAACTTCGGAGTTCCTACGGAACCTAAAGCCAGTGAGGTCCCAAAAGGCCTCATCCACTCCCCTGGGCGATGTGGGATGTAACAATCCACCCCCCTTAGGGGCCCAATGTCCTCGTCGGCACACTTCCGGCCAAGGATTGGCTCTGATACCGAATTGTCACATCCTGGCCCAGGCCCCCACCACATCCCTGGCTCGACTCCATCATAGCACGATATTGTATGCTTTTGGCCCCGACCACGCCCTCACGGTTTTGTTTCTGGGAACTCGAGTTCCTACGGAACCTGAAGCCAGTGAGCTCCCAAAAGGCCTCGTGCTAGGTAGAGATGGGAATATACATACAAGGCTTACAGGATCCACTCACCTAGGTGATGTGGGATATAACAATCCACCCCCCTTAGGGGCCCGATGTCCTCGTCGGCACACTTCCGGCCAAGGATTGGCTCTGATACCAAATTGTCACATCCCGACTCGAGCCCCCACCATATCCCTGGCTCGACTCCACCATAGCACGATATTGTCCGTTTTCAGCCCCGACCACGCCCTCACGGTTTTGTTTCTGGGAACTCACACGAGAACTTTCCAGTGGGTCATCCATCCTGGGATTGCCCTCAAACTTCTCCCAAAAGGCCTCGTGTTAGGTAGAGATGGGAATATACATATAAGGCTTATAGGATCCACTCTCCTGGATGATTTTAATGGAGGAGATAGGGAGATGGTTAATACTGGTAGAATCTTAGAAAGAAGTGAGAATAGGGAGACAAAAATGAAAAACATAAATTTTGGAGAAATTGTAATAATCAAACCCTTTGATCATTCAACTTGTGTAGATACAATCATATTTGGTTTATCTATTTATTTATACAAGGATTATGAAAGTCATTTCTTAGTTTGCATTTTCTTTACACTACTAAGATTTTCCATGTGTTGGTGTGCGTGCGATTTTTGTGTCTTAAGATTGGTTAGCCACTTTTTGGTTCAGTTTGGGTTTAAAGAGTACTTCCTCTTTTCATGTGCTCTTGCAAAGTTTGAGGATGATCCCTTAGCTTTATGTGTACGTGTCAACTGTGCCATGAGTCATAACTTATTTTACCAAGAGTTGACAAATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

816

Amino Acids

92.55

Weight (kDa)

9.3

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000158)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10163
malus_domestica MD04G1068000.v1.1 MD05G1130900.v1.1 MD06G1153600.v1.1 MD10G1255900.v1.1 MD10G1282000.v1.1 MD13G1075400.v1.1 MD13G1192400.v1.1
pyrus_communis pycom01g00130 pycom01g00390 pycom01g00700 pycom01g00720 pycom01g00750 pycom01g00850 pycom01g00870 pycom01g00910 pycom01g01250 pycom01g01560 pycom01g01680 pycom01g01860 pycom01g01900 pycom01g01910 pycom01g02200 pycom01g02260 pycom01g02270 pycom01g02280 pycom01g02330 pycom01g02480 pycom01g02720 pycom01g02730 pycom01g02750 pycom01g02770 pycom01g02780 pycom01g02860 pycom01g02910 pycom01g03540 pycom01g04090 pycom01g04100 pycom01g04150 pycom01g04250 pycom01g04430 pycom01g11810 pycom01g11860 pycom01g11940 pycom01g21380 pycom02g06650 pycom02g08490 pycom02g08600 pycom02g18230 pycom02g18450 pycom02g18800 pycom02g19150 pycom02g20740 pycom02g21340 pycom02g26210 pycom03g12060 pycom03g19060 pycom03g19100 pycom03g19120 pycom04g06890 pycom04g08960 pycom05g01790 pycom05g07910 pycom05g08080 pycom05g08100 pycom05g08570 pycom05g21500 pycom05g22400 pycom05g22510 pycom05g22620 pycom05g23020 pycom05g23720 pycom05g23890 pycom05g24040 pycom07g12080 pycom08g07930 pycom08g11940 pycom09g12120 pycom10g03890 pycom10g23360 pycom10g28250 pycom11g02850 pycom11g14370 pycom11g14400 pycom11g21840 pycom11g21860 pycom11g21980 pycom11g23580 pycom11g23650 pycom11g24050 pycom11g25610 pycom12424g00060 pycom1256g00130 pycom12661g00030 pycom12g09520 pycom12g09530 pycom12g09660 pycom12g09730 pycom12g11210 pycom12g16710 pycom12g16740 pycom12g16750 pycom13g08800 pycom13g18580 pycom13g18670 pycom13g18770 pycom13g22900 pycom13g23050 pycom13g23160 pycom13g23480 pycom13g23610 pycom13g23660 pycom13g23790 pycom13g23840 pycom13g24010 pycom13g24140 pycom13g24210 pycom13g24300 pycom13g24310 pycom13g24480 pycom13g24520 pycom13g24570 pycom13g24730 pycom13g24760 pycom13g24820 pycom13g25010 pycom13g25020 pycom13g25270 pycom13g25620 pycom13g25670 pycom13g25760 pycom13g25800 pycom13g25900 pycom13g26620 pycom13g26640 pycom13g26670 pycom13g26940 pycom13g27040 pycom13g27130 pycom13g27530 pycom13g27770 pycom13g27830 pycom13g27920 pycom13g27940 pycom13g28100 pycom13g28420 pycom13g28430 pycom13g28460 pycom13g28520 pycom13g29160 pycom14g05380 pycom15g03480 pycom15g04370 pycom15g27500 pycom16g13590 pycom16g13610 pycom16g13630 pycom16g14390 pycom16g18310 pycom17g01940 pycom17g04000 pycom17g16100 pycom17g16220 pycom17g16230 pycom17g16340 pycom17g16430 pycom17g16460 pycom17g16740 pycom17g16960 pycom17g17020 pycom17g17090 pycom17g17100 pycom17g17170 pycom17g17430 pycom17g17850 pycom17g18230 pycom17g18250 pycom17g18380 pycom17g21170 pycom17g21330 pycom520g00150 pycom520g00160 pycom808g00030 pycom808g00160 pycom808g00250 pycom808g00290
rosa_chinensis RchiOBHm_Chr5g0054451

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 152
AbsI CCTCGAGG 1 cut(s) 424
AccB1I GGYRCC 2 cut(s) 31, 741
AccB7I CCANNNNNTGG 4 cut(s) 1474, 1774, 2262, 2308
AccII CGCG 4 cut(s) 69, 227, 1325, 1327
AclWI GGATC 6 cut(s) 1065, 1683, 1696, 2004, 2017, 2366
AcoI YGGCCR 3 cut(s) 340, 1465, 1765
AcuI CTGAAG 2 cut(s) 992, 1641
AcvI CACGTG 1 cut(s) 405
AfaI GTAC 6 cut(s) 120, 601, 656, 1154, 2333, 2391
AflII CTTAAG 2 cut(s) 1121, 2287
AflIII ACRYGT 1 cut(s) 2392
AgsI TTSAA 9 cut(s) 103, 141, 221, 484, 596, 691, 697, 705, 2154
AjnI CCWGG 7 cut(s) 1398, 1500, 1506, 1524, 1824, 1933, 2019
AjuI GAANNNNNNNTTGG 2 cut(s) 562, 594
AleI CACNNNNGTG 2 cut(s) 29, 1701
AloI GAACNNNNNNTCC 4 cut(s) 1590, 1591, 1622, 1623
AluBI AGCT 3 cut(s) 507, 1633, 2381
AluI AGCT 3 cut(s) 507, 1633, 2381
Alw21I GWGCWC 4 cut(s) 404, 509, 1635, 2353
Alw26I GTCTC 1 cut(s) 2087
Alw44I GTGCAC 1 cut(s) 400
AlwI GGATC 6 cut(s) 1065, 1683, 1696, 2004, 2017, 2366
Ama87I CYCGRG 3 cut(s) 424, 1602, 1806
ApaI GGGCCC 3 cut(s) 1244, 1441, 1741
ApaLI GTGCAC 1 cut(s) 400
ApeKI GCWGC 2 cut(s) 5, 50
ArsI GACNNNNNNTTYG 2 cut(s) 96, 128
AseI ATTAAT 1 cut(s) 1050
Asp700I GAANNNNTTC 1 cut(s) 496
AspA2I CCTAGG 2 cut(s) 1310, 1699
AspLEI GCGC 3 cut(s) 227, 1106, 1327
AsuHPI GGTGA 3 cut(s) 769, 1688, 1715
AvaI CYCGRG 3 cut(s) 424, 1602, 1806
AvaII GGWCC 1 cut(s) 1374
AvrII CCTAGG 2 cut(s) 1310, 1699
AxyI CCTNAGG 2 cut(s) 1432, 1732
BaeGI GKGCMC 6 cut(s) 36, 404, 744, 1244, 1441, 1741
BamHI GGATCC 2 cut(s) 1688, 2009
BanI GGYRCC 2 cut(s) 31, 741
BanII GRGCYC 6 cut(s) 509, 1244, 1441, 1635, 1741, 1813
BauI CACGAG 3 cut(s) 1646, 1906, 1967
BbrPI CACGTG 1 cut(s) 405
BbsI GAAGAC 2 cut(s) 224, 1198
Bbv12I GWGCWC 4 cut(s) 404, 509, 1635, 2353
BbvI GCAGC 1 cut(s) 37
BccI CCATC 9 cut(s) 22, 304, 932, 1314, 1545, 1655, 1938, 1976, 2044
BceAI ACGGC 1 cut(s) 367
BciT130I CCWGG 7 cut(s) 1400, 1502, 1508, 1526, 1826, 1935, 2021
BclI TGATCA 1 cut(s) 2146
BcoDI GTCTC 1 cut(s) 2087
BfaI CTAG 5 cut(s) 167, 864, 1311, 1652, 1700
BfrI CTTAAG 2 cut(s) 1121, 2287
BisI GCNGC 2 cut(s) 6, 51
BlnI CCTAGG 2 cut(s) 1310, 1699
BlsI GCNGC 2 cut(s) 7, 52
BmcAI AGTACT 2 cut(s) 601, 2333
Bme1390I CCNGG 7 cut(s) 1400, 1502, 1508, 1526, 1826, 1935, 2021
Bme18I GGWCC 1 cut(s) 1374
BmeT110I CYCGRG 3 cut(s) 424, 1602, 1806
BmrFI CCNGG 7 cut(s) 1400, 1502, 1508, 1526, 1826, 1935, 2021
BmrI ACTGGG 2 cut(s) 1220, 1288
BmsI GCATC 1 cut(s) 346
BmuI ACTGGG 2 cut(s) 1220, 1288
BpiI GAAGAC 2 cut(s) 224, 1198
Bpu10I CCTNAGC 1 cut(s) 2377
Bsa29I ATCGAT 1 cut(s) 524
BsaAI YACGTR 2 cut(s) 405, 2393
BsaXI ACNNNNNCTCC 4 cut(s) 1130, 1160, 2112, 2142
Bse1I ACTGG 6 cut(s) 1226, 1294, 1367, 1626, 1918, 2065
Bse21I CCTNAGG 2 cut(s) 1432, 1732
BseBI CCWGG 7 cut(s) 1400, 1502, 1508, 1526, 1826, 1935, 2021
BseCI ATCGAT 1 cut(s) 524
BseMII CTCAG 1 cut(s) 1262
BseNI ACTGG 6 cut(s) 1226, 1294, 1367, 1626, 1918, 2065
BseRI GAGGAG 1 cut(s) 2052
BseSI GKGCMC 6 cut(s) 36, 404, 744, 1244, 1441, 1741
BseXI GCAGC 1 cut(s) 37
BseYI CCCAGC 1 cut(s) 1220
Bsh1236I CGCG 4 cut(s) 69, 227, 1325, 1327
BshNI GGYRCC 2 cut(s) 31, 741
BshVI ATCGAT 1 cut(s) 524
BsiHKAI GWGCWC 4 cut(s) 404, 509, 1635, 2353
BsiHKCI CYCGRG 3 cut(s) 424, 1602, 1806
BsiSI CCGG 2 cut(s) 1464, 1764
BslFI GGGAC 2 cut(s) 438, 1360
BsmAI GTCTC 1 cut(s) 2087
BsmFI GGGAC 2 cut(s) 438, 1360
BsmI GAATGC 2 cut(s) 227, 287
BsoBI CYCGRG 3 cut(s) 424, 1602, 1806
Bsp120I GGGCCC 3 cut(s) 1240, 1437, 1737
Bsp143I GATC 5 cut(s) 1070, 1688, 2009, 2146, 2371
Bsp19I CCATGG 3 cut(s) 37, 1096, 1198
BspCNI CTCAG 1 cut(s) 1263
BspDI ATCGAT 1 cut(s) 524
BspFNI CGCG 4 cut(s) 69, 227, 1325, 1327
BspHI TCATGA 1 cut(s) 1009
BspPI GGATC 6 cut(s) 1065, 1683, 1696, 2004, 2017, 2366
BspT107I GGYRCC 2 cut(s) 31, 741
BspTI CTTAAG 2 cut(s) 1121, 2287
BsrI ACTGG 6 cut(s) 1226, 1294, 1367, 1626, 1918, 2065
BssMI GATC 5 cut(s) 1070, 1688, 2009, 2146, 2371
BssSI CACGAG 3 cut(s) 1646, 1906, 1967
BssT1I CCWWGG 8 cut(s) 37, 322, 1096, 1198, 1310, 1468, 1699, 1768
Bst2BI CACGAG 3 cut(s) 1646, 1906, 1967
Bst2UI CCWGG 7 cut(s) 1400, 1502, 1508, 1526, 1826, 1935, 2021
Bst4CI ACNGT 6 cut(s) 13, 1110, 1261, 1585, 1885, 2402
BstAFI CTTAAG 2 cut(s) 1121, 2287
BstBAI YACGTR 2 cut(s) 405, 2393
BstC8I GCNNGC 2 cut(s) 400, 2272
BstDEI CTNAG 8 cut(s) 603, 1271, 1432, 1732, 2071, 2221, 2246, 2377
BstDSI CCRYGG 3 cut(s) 37, 1096, 1198
BstEII GGTNACC 1 cut(s) 1300
BstENI CCTNNNNNAGG 2 cut(s) 326, 1505
BstFNI CGCG 4 cut(s) 69, 227, 1325, 1327
BstHHI GCGC 3 cut(s) 227, 1106, 1327
BstKTI GATC 5 cut(s) 1073, 1691, 2012, 2149, 2374
BstMAI GTCTC 1 cut(s) 2087
BstMBI GATC 5 cut(s) 1070, 1688, 2009, 2146, 2371
BstMWI GCNNNNNNNGC 1 cut(s) 1110
BstNI CCWGG 7 cut(s) 1400, 1502, 1508, 1526, 1826, 1935, 2021
BstNSI RCATGY 1 cut(s) 246
BstPI GGTNACC 1 cut(s) 1300
BstSCI CCNGG 7 cut(s) 1398, 1500, 1506, 1524, 1824, 1933, 2019
BstSLI GKGCMC 6 cut(s) 36, 404, 744, 1244, 1441, 1741
BstUI CGCG 4 cut(s) 69, 227, 1325, 1327
BstV1I GCAGC 1 cut(s) 37
BstV2I GAAGAC 2 cut(s) 224, 1198
BstX2I RGATCY 3 cut(s) 1070, 1688, 2009
BstXI CCANNNNNNTGG 5 cut(s) 44, 1399, 1525, 1825, 2020
BstYI RGATCY 3 cut(s) 1070, 1688, 2009
Bsu15I ATCGAT 1 cut(s) 524
Bsu36I CCTNAGG 2 cut(s) 1432, 1732
BsuTUI ATCGAT 1 cut(s) 524
BtgI CCRYGG 3 cut(s) 37, 1096, 1198
BtgZI GCGATG 1 cut(s) 1419
BtsIMutI CAGTG 5 cut(s) 1233, 1301, 1374, 1633, 1925
Cac8I GCNNGC 2 cut(s) 400, 2272
CciI TCATGA 1 cut(s) 1009
CfoI GCGC 3 cut(s) 227, 1106, 1327
ClaI ATCGAT 1 cut(s) 524
CseI GACGC 2 cut(s) 65, 799
Csp6I GTAC 6 cut(s) 119, 600, 655, 1153, 2332, 2390
CviQI GTAC 6 cut(s) 119, 600, 655, 1153, 2332, 2390
DdeI CTNAG 8 cut(s) 603, 1271, 1432, 1732, 2071, 2221, 2246, 2377
DpnI GATC 5 cut(s) 1072, 1690, 2011, 2148, 2373
DpnII GATC 5 cut(s) 1070, 1688, 2009, 2146, 2371
DraI TTTAAA 2 cut(s) 990, 2326
EaeI YGGCCR 3 cut(s) 340, 1465, 1765
Ecl136II GAGCTC 2 cut(s) 507, 1633
Eco130I CCWWGG 8 cut(s) 37, 322, 1096, 1198, 1310, 1468, 1699, 1768
Eco147I AGGCCT 3 cut(s) 1385, 1644, 1965
Eco24I GRGCYC 6 cut(s) 509, 1244, 1441, 1635, 1741, 1813
Eco47I GGWCC 1 cut(s) 1374
Eco53kI GAGCTC 2 cut(s) 507, 1633
Eco57I CTGAAG 2 cut(s) 992, 1641
Eco72I CACGTG 1 cut(s) 405
Eco81I CCTNAGG 2 cut(s) 1432, 1732
Eco88I CYCGRG 3 cut(s) 424, 1602, 1806
Eco91I GGTNACC 1 cut(s) 1300
EcoICRI GAGCTC 2 cut(s) 507, 1633
EcoNI CCTNNNNNAGG 2 cut(s) 326, 1505
EcoO109I RGGNCCY 5 cut(s) 1241, 1374, 1437, 1510, 1737
EcoO65I GGTNACC 1 cut(s) 1300
EcoRII CCWGG 7 cut(s) 1398, 1500, 1506, 1524, 1824, 1933, 2019
EcoT14I CCWWGG 8 cut(s) 37, 322, 1096, 1198, 1310, 1468, 1699, 1768
EcoT38I GRGCYC 6 cut(s) 509, 1244, 1441, 1635, 1741, 1813
ErhI CCWWGG 8 cut(s) 37, 322, 1096, 1198, 1310, 1468, 1699, 1768
FaqI GGGAC 2 cut(s) 438, 1360
FbaI TGATCA 1 cut(s) 2146
Fnu4HI GCNGC 2 cut(s) 6, 51
FriOI GRGCYC 6 cut(s) 509, 1244, 1441, 1635, 1741, 1813
Fsp4HI GCNGC 2 cut(s) 6, 51
FspBI CTAG 5 cut(s) 167, 864, 1311, 1652, 1700
GlaI GCGC 3 cut(s) 226, 1105, 1326
GluI GCNGC 2 cut(s) 6, 51
GsaI CCCAGC 1 cut(s) 1224
HapII CCGG 2 cut(s) 1464, 1764
HgaI GACGC 2 cut(s) 65, 799
HhaI GCGC 3 cut(s) 227, 1106, 1327
Hin6I GCGC 3 cut(s) 225, 1104, 1325
HinP1I GCGC 3 cut(s) 225, 1104, 1325
HincII GTYRAC 2 cut(s) 2398, 2436
HindII GTYRAC 2 cut(s) 2398, 2436
HpaII CCGG 2 cut(s) 1464, 1764
HphI GGTGA 3 cut(s) 769, 1688, 1715
Hpy166II GTNNAC 4 cut(s) 402, 2390, 2398, 2436
Hpy188I TCNGA 6 cut(s) 355, 972, 1272, 1345, 1482, 1782
Hpy188III TCNNGA 6 cut(s) 252, 931, 1010, 1040, 1164, 1801
Hpy8I GTNNAC 4 cut(s) 402, 2390, 2398, 2436
Hpy99I CGWCG 2 cut(s) 1455, 1755
HpyAV CCTTC 1 cut(s) 1046
HpyCH4III ACNGT 6 cut(s) 13, 1110, 1261, 1585, 1885, 2402
HpyCH4IV ACGT 2 cut(s) 404, 2392
HpyCH4V TGCA 4 cut(s) 285, 402, 2229, 2357
HpyF10VI GCNNNNNNNGC 1 cut(s) 1110
HpyF3I CTNAG 8 cut(s) 603, 1271, 1432, 1732, 2071, 2221, 2246, 2377
HpySE526I ACGT 2 cut(s) 404, 2392
HspAI GCGC 3 cut(s) 225, 1104, 1325
Ksp22I TGATCA 1 cut(s) 2146
Kzo9I GATC 5 cut(s) 1070, 1688, 2009, 2146, 2371
LmnI GCTCC 6 cut(s) 58, 324, 504, 826, 1138, 1638
Lsp1109I GCAGC 1 cut(s) 37
LweI GCATC 1 cut(s) 346
MaeI CTAG 5 cut(s) 167, 864, 1311, 1652, 1700
MaeII ACGT 2 cut(s) 404, 2392
MaeIII GTNAC 6 cut(s) 1027, 1212, 1300, 1415, 1493, 1793
MalI GATC 5 cut(s) 1072, 1690, 2011, 2148, 2373
MboI GATC 5 cut(s) 1070, 1688, 2009, 2146, 2371
MboII GAAGA 7 cut(s) 224, 227, 291, 357, 523, 533, 1203
MflI RGATCY 3 cut(s) 1070, 1688, 2009
MlyI GAGTC 7 cut(s) 238, 249, 1188, 1527, 1798, 1827, 2419
MmeI TCCRAC 2 cut(s) 80, 1036
MroXI GAANNNNTTC 1 cut(s) 496
MslI CAYNNNNRTG 3 cut(s) 29, 42, 1701
MspCI CTTAAG 2 cut(s) 1121, 2287
MspI CCGG 2 cut(s) 1464, 1764
MspR9I CCNGG 7 cut(s) 1400, 1502, 1508, 1526, 1826, 1935, 2021
Mva1269I GAATGC 2 cut(s) 227, 287
MvaI CCWGG 7 cut(s) 1400, 1502, 1508, 1526, 1826, 1935, 2021
MvnI CGCG 4 cut(s) 69, 227, 1325, 1327
MwoI GCNNNNNNNGC 1 cut(s) 1110
NcoI CCATGG 3 cut(s) 37, 1096, 1198
NdeII GATC 5 cut(s) 1070, 1688, 2009, 2146, 2371
NmuCI GTSAC 3 cut(s) 1212, 1493, 1793
NspI RCATGY 1 cut(s) 246
OliI CACNNNNGTG 2 cut(s) 29, 1701
PaeR7I CTCGAG 3 cut(s) 424, 1602, 1806
PagI TCATGA 1 cut(s) 1009
PasI CCCWGGG 1 cut(s) 1399
PceI AGGCCT 3 cut(s) 1385, 1644, 1965
PcsI WCGNNNNNNNCGW 1 cut(s) 73
PctI GAATGC 2 cut(s) 227, 287
PdmI GAANNNNTTC 1 cut(s) 496
PfeI GAWTC 4 cut(s) 248, 348, 410, 2067
PflMI CCANNNNNTGG 4 cut(s) 1474, 1774, 2262, 2308
PfoI TCCNGGA 1 cut(s) 2019
PkrI GCNGC 2 cut(s) 7, 52
PleI GAGTC 7 cut(s) 237, 249, 1188, 1527, 1798, 1827, 2418
PmaCI CACGTG 1 cut(s) 405
PmlI CACGTG 1 cut(s) 405
PpsI GAGTC 7 cut(s) 237, 249, 1188, 1527, 1798, 1827, 2418
Ppu21I YACGTR 2 cut(s) 405, 2393
PpuMI RGGWCCY 1 cut(s) 1374
PshBI ATTAAT 1 cut(s) 1050
PsiI TTATAA 1 cut(s) 152
Psp124BI GAGCTC 2 cut(s) 509, 1635
Psp5II RGGWCCY 1 cut(s) 1374
Psp6I CCWGG 7 cut(s) 1398, 1500, 1506, 1524, 1824, 1933, 2019
PspCI CACGTG 1 cut(s) 405
PspEI GGTNACC 1 cut(s) 1300
PspFI CCCAGC 1 cut(s) 1220
PspGI CCWGG 7 cut(s) 1398, 1500, 1506, 1524, 1824, 1933, 2019
PspOMI GGGCCC 3 cut(s) 1240, 1437, 1737
PspPPI RGGWCCY 1 cut(s) 1374
PspXI VCTCGAGB 3 cut(s) 424, 1602, 1806
PsuI RGATCY 3 cut(s) 1070, 1688, 2009
RsaI GTAC 6 cut(s) 120, 601, 656, 1154, 2333, 2391
RsaNI GTAC 6 cut(s) 119, 600, 655, 1153, 2332, 2390
RseI CAYNNNNRTG 3 cut(s) 29, 42, 1701
SacI GAGCTC 2 cut(s) 509, 1635
SatI GCNGC 2 cut(s) 6, 51
Sau3AI GATC 5 cut(s) 1070, 1688, 2009, 2146, 2371
ScaI AGTACT 2 cut(s) 601, 2333
SchI GAGTC 7 cut(s) 238, 249, 1188, 1527, 1798, 1827, 2419
ScrFI CCNGG 7 cut(s) 1400, 1502, 1508, 1526, 1826, 1935, 2021
SfaNI GCATC 1 cut(s) 346
Sfr274I CTCGAG 3 cut(s) 424, 1602, 1806
SinI GGWCC 1 cut(s) 1374
SlaI CTCGAG 3 cut(s) 424, 1602, 1806
SmiMI CAYNNNNRTG 3 cut(s) 29, 42, 1701
SmlI CTYRAG 5 cut(s) 424, 1121, 1602, 1806, 2287
SmoI CTYRAG 5 cut(s) 424, 1121, 1602, 1806, 2287
SseBI AGGCCT 3 cut(s) 1385, 1644, 1965
SspMI CTAG 5 cut(s) 167, 864, 1311, 1652, 1700
SstI GAGCTC 2 cut(s) 509, 1635
StuI AGGCCT 3 cut(s) 1385, 1644, 1965
StyD4I CCNGG 7 cut(s) 1398, 1500, 1506, 1524, 1824, 1933, 2019
StyI CCWWGG 8 cut(s) 37, 322, 1096, 1198, 1310, 1468, 1699, 1768
TaaI ACNGT 6 cut(s) 13, 1110, 1261, 1585, 1885, 2402
TaiI ACGT 2 cut(s) 407, 2395
TaqI TCGA 8 cut(s) 238, 425, 524, 930, 1531, 1603, 1807, 1831
TatI WGTACW 4 cut(s) 118, 599, 654, 2331
TfiI GAWTC 4 cut(s) 248, 348, 410, 2067
TscAI CASTG 5 cut(s) 1233, 1301, 1374, 1633, 1925
TseFI GTSAC 3 cut(s) 1212, 1493, 1793
TseI GCWGC 2 cut(s) 5, 50
Tsp45I GTSAC 3 cut(s) 1212, 1493, 1793
TspGWI ACGGA 5 cut(s) 382, 1222, 1369, 1628, 1846
TspRI CASTG 5 cut(s) 1233, 1301, 1374, 1633, 1925
Van91I CCANNNNNTGG 4 cut(s) 1474, 1774, 2262, 2308
Vha464I CTTAAG 2 cut(s) 1121, 2287
VneI GTGCAC 1 cut(s) 400
VpaK11BI GGWCC 1 cut(s) 1374
VspI ATTAAT 1 cut(s) 1050
XagI CCTNNNNNAGG 2 cut(s) 326, 1505
XceI RCATGY 1 cut(s) 246
XcmI CCANNNNNNNNNTGG 2 cut(s) 1522, 1822
XhoI CTCGAG 3 cut(s) 424, 1602, 1806
XmaJI CCTAGG 2 cut(s) 1310, 1699
XmnI GAANNNNTTC 1 cut(s) 496
XspI CTAG 5 cut(s) 167, 864, 1311, 1652, 1700
ZrmI AGTACT 2 cut(s) 601, 2333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.