pycom17g16220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000158
Physical Location & Seq
Forward (+)
14439264 .. 14441802
2539 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g16220.5

Sequence Viewer

Length: 1920 bp
ATGAGTCTAGAGGATGAAATTGAAGGCGCGATGGCTTGTTTTCATCTGAAAATAAAGTGCCTAATTGAAAAATTAAAGAATTTAGGCGCTAAATTTTATTCTATTGTTATTTACGATGTTACTTACACAAGAGTCGGGTATACTTTTGAGCTAACGTTTAGTGTTCAAATTTTATTTTATTTGCGACGGCCATGTTATCTGTCGCAAAAGGCCCTTTTTCATGAAGTGAATTACAATTATGGTGCTATATATAGATTCTATCTTTTTCCCTGGATATATATTTTTTTATTAATTGAAAAGAATCCTACCATCCTCTTATCTCCTAATCTCTTCTACCATATGAAAGATAAGTATTATGTTGTTATTACATCATTTTATATAATTTACCATGTCAACATAAATTATCATTTACTAATATTTATCTTTTGTGATTTATCTAGAATTCGGTTGCTACTTATATGCTGCTACAACCATTCACTGATCACATATATAAAGGTTGCGTGTCACCAATCAGCTTATATGAAATATCATGCTGCCAATCGAACGACAATCCTATTAGAGGATCTCCTATATGTGTTGTTCAAATTAGAAAGAAAAACTGTATTTAAACTAGCTACCATACTCTGTGGAGGTTACAAATTTTTAATTAGTTTATGCCAATTAAGAATTTTGATCAATATTATTTTGAATTATTATATACATTTATTCTGTTACATCCTAATGATATTTCATTACTATGAATTTGTTGGGGTATTTATTCAAGTTTTCCGGGAGCTAAAAGTAATTTTTCATTTTATCACTAATGAGTTTCATCAAGTAATTTCAAAAACACTTAGACTATTATTTGAGACTTTCCTAAATTGGTGGGGGTGGTCATTGGAGCAAGTTTTGCATAATAATATTAATTTATTGACATTTCATGTGTGTTCAATTTCCTTATCAATGAGCTTTAGACTAGTTTCAAACTTGGTGCAAAAGCCACTGTTGGCCTTGAGAATACTATATAATTTGTGGGGTTTCTTTTATTCATTAAGTGGCATTAACTTATTGTGCAAATTTTCTCGCATGATCGATAACTTGTTCATGGCAGTGCCATTCTCAATAAATTATCTGTGCTTTTTGAGGCCAAAAAGCACCAACAATTGTGCTCATAGACCACTAGCAACAGTGCAGCTACTATCTTTGTGTTTACAGCCATGTGTGCTCAAAGAGTTGAGCACAAATAAGGTTTTTTTTTTGCCCTGTTCTGACACCTGTGTAAGATGCCATTTCAGATTCATATTAGCACAAAAATTTTGTAATTTCAAAATACCCTACAATCTGAAACAAACATTTGAAAATGTATATTCATCCTTTCAGGCAATCATCCTTCTAGTTGAGGCACCTGTGCTCCATGCATCATCCACGGCAGTACCACACGCAGCGACAGTGCGACCTGCAGCCATGCTAGGGGTTTCAGAGTTCGTTTTGGTCCAAAACACAACCATTTTCAACAATTCAAAAGCGATGACCTCCCAATTTCGCAACTTTCTGATACAAAGCGATGACCTTCTCCTTGAGACCCCTCCCCAGTATATGAGCCTGGCGTCCACCATAGACATGGGGGACCCAAAAGCTGCACCCCTCAGAAACGCGTCGAGCTCTTTGTCTGTATTCGAACTTACTCATCCGCGGCTGTGCTTGAACCGCTTCCTCCACCACATGTACATCATCACATGCCTCAGCTACTGCAGCGCCTCGCACCAAGCCTTGGACACCAACAACGGCGCTCAGGCTTGGATGGCTGAGCGTCAGCTTAATTCAGCAACAGAAAGGAGGGAAGATGGTTGTAGTGCCTTACAAAATCTCCGATTGTGCTCCGGGATCTCCAACTATGCTCCAAAGTTTCCGATGATTCCCCCAAAATCTCTGATTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

640

Amino Acids

74.31

Weight (kDa)

9.02

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000158)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10163
malus_domestica MD04G1068000.v1.1 MD05G1130900.v1.1 MD06G1153600.v1.1 MD10G1255900.v1.1 MD10G1282000.v1.1 MD13G1075400.v1.1 MD13G1192400.v1.1
pyrus_communis pycom01g00130 pycom01g00390 pycom01g00700 pycom01g00720 pycom01g00750 pycom01g00850 pycom01g00870 pycom01g00910 pycom01g01250 pycom01g01560 pycom01g01680 pycom01g01860 pycom01g01900 pycom01g01910 pycom01g02200 pycom01g02260 pycom01g02270 pycom01g02280 pycom01g02330 pycom01g02480 pycom01g02720 pycom01g02730 pycom01g02750 pycom01g02770 pycom01g02780 pycom01g02860 pycom01g02910 pycom01g03540 pycom01g04090 pycom01g04100 pycom01g04150 pycom01g04250 pycom01g04430 pycom01g11810 pycom01g11860 pycom01g11940 pycom01g21380 pycom02g06650 pycom02g08490 pycom02g08600 pycom02g18230 pycom02g18450 pycom02g18800 pycom02g19150 pycom02g20740 pycom02g21340 pycom02g26210 pycom03g12060 pycom03g19060 pycom03g19100 pycom03g19120 pycom04g06890 pycom04g08960 pycom05g01790 pycom05g07910 pycom05g08080 pycom05g08100 pycom05g08570 pycom05g21500 pycom05g22400 pycom05g22510 pycom05g22620 pycom05g23020 pycom05g23720 pycom05g23890 pycom05g24040 pycom07g12080 pycom08g07930 pycom08g11940 pycom09g12120 pycom10g03890 pycom10g23360 pycom10g28250 pycom11g02850 pycom11g14370 pycom11g14400 pycom11g21840 pycom11g21860 pycom11g21980 pycom11g23580 pycom11g23650 pycom11g24050 pycom11g25610 pycom12424g00060 pycom1256g00130 pycom12661g00030 pycom12g09520 pycom12g09530 pycom12g09660 pycom12g09730 pycom12g11210 pycom12g16710 pycom12g16740 pycom12g16750 pycom13g08800 pycom13g18580 pycom13g18670 pycom13g18770 pycom13g22900 pycom13g23050 pycom13g23160 pycom13g23480 pycom13g23610 pycom13g23660 pycom13g23790 pycom13g23840 pycom13g24010 pycom13g24140 pycom13g24210 pycom13g24300 pycom13g24310 pycom13g24480 pycom13g24520 pycom13g24570 pycom13g24730 pycom13g24760 pycom13g24820 pycom13g25010 pycom13g25020 pycom13g25270 pycom13g25620 pycom13g25670 pycom13g25760 pycom13g25800 pycom13g25900 pycom13g26620 pycom13g26640 pycom13g26670 pycom13g26940 pycom13g27040 pycom13g27130 pycom13g27530 pycom13g27770 pycom13g27830 pycom13g27920 pycom13g27940 pycom13g28100 pycom13g28420 pycom13g28430 pycom13g28460 pycom13g28520 pycom13g29160 pycom14g05380 pycom15g03480 pycom15g04370 pycom15g27500 pycom16g13590 pycom16g13610 pycom16g13630 pycom16g14390 pycom16g18310 pycom17g01940 pycom17g04000 pycom17g16100 pycom17g16220 pycom17g16230 pycom17g16340 pycom17g16430 pycom17g16460 pycom17g16740 pycom17g16960 pycom17g17020 pycom17g17090 pycom17g17100 pycom17g17170 pycom17g17430 pycom17g17850 pycom17g18230 pycom17g18250 pycom17g18380 pycom17g21170 pycom17g21330 pycom520g00150 pycom520g00160 pycom808g00030 pycom808g00160 pycom808g00250 pycom808g00290
rosa_chinensis RchiOBHm_Chr5g0054451

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1444
AccB1I GGYRCC 1 cut(s) 1381
AccB7I CCANNNNNTGG 1 cut(s) 1750
AccI GTMKAC 1 cut(s) 140
AccII CGCG 3 cut(s) 29, 1634, 1672
AciI CCGC 3 cut(s) 1670, 1672, 1687
AclI AACGTT 1 cut(s) 155
AclWI GGATC 2 cut(s) 570, 1871
AcoI YGGCCR 1 cut(s) 188
AcsI RAATTY 9 cut(s) 79, 92, 168, 441, 638, 666, 740, 1055, 1292
AcyI GRCGYC 1 cut(s) 1586
AfaI GTAC 2 cut(s) 1413, 1706
AfiI CCNNNNNNNGG 3 cut(s) 559, 1449, 1750
AflIII ACRYGT 2 cut(s) 1632, 1701
AhlI ACTAGT 1 cut(s) 955
AjnI CCWGG 2 cut(s) 269, 1581
Alw21I GWGCWC 6 cut(s) 1150, 1206, 1220, 1392, 1643, 1859
Alw26I GTCTC 2 cut(s) 842, 1553
AlwI GGATC 2 cut(s) 570, 1871
AlwNI CAGNNNCTG 1 cut(s) 1728
AoxI GGCC 4 cut(s) 188, 210, 987, 1124
ApeKI GCWGC 7 cut(s) 462, 533, 1171, 1421, 1439, 1616, 1731
ApoI RAATTY 9 cut(s) 79, 92, 168, 441, 638, 666, 740, 1055, 1292
AseI ATTAAT 2 cut(s) 290, 903
Asp700I GAANNNNTTC 1 cut(s) 1688
AspLEI GCGC 4 cut(s) 29, 89, 1736, 1769
AspS9I GGNCC 3 cut(s) 211, 1471, 1606
AsuC2I CCSGG 2 cut(s) 770, 1861
AsuHPI GGTGA 1 cut(s) 497
AsuII TTCGAA 1 cut(s) 1656
AvaII GGWCC 2 cut(s) 1471, 1606
BaeI ACNNNNGTAYC 2 cut(s) 1395, 1428
BanI GGYRCC 1 cut(s) 1381
BanII GRGCYC 1 cut(s) 1643
Bbv12I GWGCWC 6 cut(s) 1150, 1206, 1220, 1392, 1643, 1859
BbvCI CCTCAGC 1 cut(s) 1721
BbvI GCAGC 7 cut(s) 449, 520, 1183, 1433, 1451, 1603, 1743
BccI CCATC 4 cut(s) 25, 317, 1774, 1817
BceAI ACGGC 3 cut(s) 203, 1422, 1780
BciT130I CCWGG 2 cut(s) 271, 1583
BclI TGATCA 2 cut(s) 480, 672
BcnI CCSGG 2 cut(s) 770, 1861
BcoDI GTCTC 2 cut(s) 842, 1553
BcuI ACTAGT 1 cut(s) 955
BfaI CTAG 7 cut(s) 8, 438, 611, 956, 1160, 1373, 1448
BfmI CTRYAG 2 cut(s) 1437, 1729
BfoI RGCGCY 3 cut(s) 90, 1737, 1770
BfuAI ACCTGC 1 cut(s) 1444
BisI GCNGC 8 cut(s) 463, 534, 1172, 1422, 1440, 1617, 1673, 1732
BlpI GCTNAGC 1 cut(s) 1785
BlsI GCNGC 8 cut(s) 464, 535, 1173, 1423, 1441, 1618, 1674, 1733
Bme1390I CCNGG 4 cut(s) 271, 770, 1583, 1861
Bme18I GGWCC 2 cut(s) 1471, 1606
BmgT120I GGNCC 3 cut(s) 211, 1471, 1606
BmiI GGNNCC 3 cut(s) 1383, 1607, 1608
BmrFI CCNGG 4 cut(s) 271, 770, 1583, 1861
BmrI ACTGGG 1 cut(s) 1564
BmsI GCATC 2 cut(s) 1253, 1406
BmuI ACTGGG 1 cut(s) 1564
Bpu10I CCTNAGC 2 cut(s) 1721, 1770
Bpu1102I GCTNAGC 1 cut(s) 1785
Bpu14I TTCGAA 1 cut(s) 1656
BpuEI CTTGAG 2 cut(s) 1012, 1577
BpuMI CCSGG 2 cut(s) 770, 1861
Bsa29I ATCGAT 1 cut(s) 1071
BsaHI GRCGYC 1 cut(s) 1586
BsaI GGTCTC 1 cut(s) 1553
BsaJI CCNNGG 4 cut(s) 269, 1404, 1670, 1749
BsaXI ACNNNNNCTCC 4 cut(s) 1374, 1404, 1830, 1860
Bsc4I CCNNNNNNNGG 3 cut(s) 559, 1449, 1750
Bse1I ACTGG 1 cut(s) 1570
BseBI CCWGG 2 cut(s) 271, 1583
BseCI ATCGAT 1 cut(s) 1071
BseDI CCNNGG 4 cut(s) 269, 1404, 1670, 1749
BseGI GGATG 8 cut(s) 19, 309, 714, 1349, 1365, 1400, 1666, 1785
BseLI CCNNNNNNNGG 3 cut(s) 559, 1449, 1750
BseMII CTCAG 4 cut(s) 1639, 1735, 1776, 1784
BseNI ACTGG 1 cut(s) 1570
BseXI GCAGC 7 cut(s) 449, 520, 1183, 1433, 1451, 1603, 1743
BsgI GTGCAG 2 cut(s) 1190, 1602
Bsh1236I CGCG 3 cut(s) 29, 1634, 1672
BshFI GGCC 4 cut(s) 190, 212, 989, 1126
BshNI GGYRCC 1 cut(s) 1381
BshVI ATCGAT 1 cut(s) 1071
BsiHKAI GWGCWC 6 cut(s) 1150, 1206, 1220, 1392, 1643, 1859
BsiSI CCGG 2 cut(s) 769, 1860
BslFI GGGAC 1 cut(s) 1619
BslI CCNNNNNNNGG 3 cut(s) 559, 1449, 1750
BsmAI GTCTC 2 cut(s) 842, 1553
BsmFI GGGAC 1 cut(s) 1619
BsnI GGCC 4 cut(s) 190, 212, 989, 1126
Bso31I GGTCTC 1 cut(s) 1553
Bsp119I TTCGAA 1 cut(s) 1656
Bsp1286I GDGCHC 6 cut(s) 1150, 1206, 1220, 1392, 1643, 1859
Bsp1407I TGTACA 1 cut(s) 1704
Bsp143I GATC 5 cut(s) 480, 562, 672, 1068, 1863
Bsp1720I GCTNAGC 1 cut(s) 1785
BspACI CCGC 3 cut(s) 1670, 1672, 1687
BspANI GGCC 4 cut(s) 190, 212, 989, 1126
BspCNI CTCAG 4 cut(s) 1638, 1734, 1777, 1783
BspDI ATCGAT 1 cut(s) 1071
BspFNI CGCG 3 cut(s) 29, 1634, 1672
BspHI TCATGA 1 cut(s) 220
BspLI GGNNCC 3 cut(s) 1383, 1607, 1608
BspMAI CTGCAG 2 cut(s) 1441, 1733
BspMI ACCTGC 1 cut(s) 1444
BspPI GGATC 2 cut(s) 570, 1871
BspT104I TTCGAA 1 cut(s) 1656
BspT107I GGYRCC 1 cut(s) 1381
BspTNI GGTCTC 1 cut(s) 1553
BsrGI TGTACA 1 cut(s) 1704
BsrI ACTGG 1 cut(s) 1570
BssECI CCNNGG 4 cut(s) 269, 1404, 1670, 1749
BssMI GATC 5 cut(s) 480, 562, 672, 1068, 1863
BssNAI GTATAC 1 cut(s) 141
BssNI GRCGYC 1 cut(s) 1586
BssT1I CCWWGG 1 cut(s) 1749
Bst1107I GTATAC 1 cut(s) 141
Bst2UI CCWGG 2 cut(s) 271, 1583
Bst4CI ACNGT 4 cut(s) 601, 984, 1168, 1429
Bst6I CTCTTC 1 cut(s) 335
BstACI GRCGYC 1 cut(s) 1586
BstAPI GCANNNNNTGC 1 cut(s) 889
BstAUI TGTACA 1 cut(s) 1704
BstBI TTCGAA 1 cut(s) 1656
BstDEI CTNAG 5 cut(s) 833, 1625, 1721, 1770, 1785
BstDSI CCRYGG 2 cut(s) 1404, 1670
BstENI CCTNNNNNAGG 1 cut(s) 557
BstF5I GGATG 8 cut(s) 19, 309, 714, 1349, 1365, 1400, 1666, 1785
BstFNI CGCG 3 cut(s) 29, 1634, 1672
BstH2I RGCGCY 3 cut(s) 90, 1737, 1770
BstHHI GCGC 4 cut(s) 29, 89, 1736, 1769
BstKTI GATC 5 cut(s) 483, 565, 675, 1071, 1866
BstMAI GTCTC 2 cut(s) 842, 1553
BstMBI GATC 5 cut(s) 480, 562, 672, 1068, 1863
BstMWI GCNNNNNNNGC 5 cut(s) 889, 1201, 1686, 1731, 1781
BstNI CCWGG 2 cut(s) 271, 1583
BstNSI RCATGY 2 cut(s) 1705, 1719
BstSCI CCNGG 4 cut(s) 269, 768, 1581, 1859
BstSFI CTRYAG 2 cut(s) 1437, 1729
BstUI CGCG 3 cut(s) 29, 1634, 1672
BstV1I GCAGC 7 cut(s) 449, 520, 1183, 1433, 1451, 1603, 1743
BstX2I RGATCY 2 cut(s) 562, 1863
BstXI CCANNNNNNTGG 1 cut(s) 1600
BstYI RGATCY 2 cut(s) 562, 1863
BstZ17I GTATAC 1 cut(s) 141
Bsu15I ATCGAT 1 cut(s) 1071
BsuRI GGCC 4 cut(s) 190, 212, 989, 1126
BsuTUI ATCGAT 1 cut(s) 1071
BtgI CCRYGG 2 cut(s) 1404, 1670
BtgZI GCGATG 3 cut(s) 44, 1520, 1557
BtsCI GGATG 8 cut(s) 19, 309, 714, 1349, 1365, 1400, 1666, 1785
BtsI GCAGTG 1 cut(s) 1095
BtsIMutI CAGTG 5 cut(s) 476, 980, 1095, 1173, 1434
BveI ACCTGC 1 cut(s) 1444
CaiI CAGNNNCTG 1 cut(s) 1728
CciI TCATGA 1 cut(s) 220
CfoI GCGC 4 cut(s) 29, 89, 1736, 1769
Cfr13I GGNCC 3 cut(s) 211, 1471, 1606
Cfr42I CCGCGG 1 cut(s) 1673
ClaI ATCGAT 1 cut(s) 1071
CseI GACGC 3 cut(s) 1575, 1623, 1778
Csp6I GTAC 2 cut(s) 1412, 1705
CviQI GTAC 2 cut(s) 1412, 1705
DdeI CTNAG 5 cut(s) 833, 1625, 1721, 1770, 1785
DpnI GATC 5 cut(s) 482, 564, 674, 1070, 1865
DpnII GATC 5 cut(s) 480, 562, 672, 1068, 1863
DraI TTTAAA 1 cut(s) 607
EaeI YGGCCR 1 cut(s) 188
Eam1104I CTCTTC 1 cut(s) 335
EarI CTCTTC 1 cut(s) 335
Ecl136II GAGCTC 1 cut(s) 1641
Eco130I CCWWGG 1 cut(s) 1749
Eco24I GRGCYC 1 cut(s) 1643
Eco31I GGTCTC 1 cut(s) 1553
Eco47I GGWCC 2 cut(s) 1471, 1606
Eco53kI GAGCTC 1 cut(s) 1641
EcoICRI GAGCTC 1 cut(s) 1641
EcoNI CCTNNNNNAGG 1 cut(s) 557
EcoO109I RGGNCCY 2 cut(s) 211, 1606
EcoRI GAATTC 1 cut(s) 441
EcoRII CCWGG 2 cut(s) 269, 1581
EcoT14I CCWWGG 1 cut(s) 1749
EcoT22I ATGCAT 1 cut(s) 1399
EcoT38I GRGCYC 1 cut(s) 1643
ErhI CCWWGG 1 cut(s) 1749
FaqI GGGAC 1 cut(s) 1619
FauNDI CATATG 1 cut(s) 339
FbaI TGATCA 2 cut(s) 480, 672
FblI GTMKAC 1 cut(s) 140
Fnu4HI GCNGC 8 cut(s) 463, 534, 1172, 1422, 1440, 1617, 1673, 1732
FokI GGATG 8 cut(s) 26, 296, 701, 1336, 1352, 1387, 1653, 1792
FriOI GRGCYC 1 cut(s) 1643
Fsp4HI GCNGC 8 cut(s) 463, 534, 1172, 1422, 1440, 1617, 1673, 1732
FspBI CTAG 7 cut(s) 8, 438, 611, 956, 1160, 1373, 1448
GlaI GCGC 4 cut(s) 28, 88, 1735, 1768
GluI GCNGC 8 cut(s) 463, 534, 1172, 1422, 1440, 1617, 1673, 1732
HaeII RGCGCY 3 cut(s) 90, 1737, 1770
HaeIII GGCC 4 cut(s) 190, 212, 989, 1126
HapII CCGG 2 cut(s) 769, 1860
HgaI GACGC 3 cut(s) 1575, 1623, 1778
HhaI GCGC 4 cut(s) 29, 89, 1736, 1769
Hin1I GRCGYC 1 cut(s) 1586
Hin6I GCGC 4 cut(s) 27, 87, 1734, 1767
HinP1I GCGC 4 cut(s) 27, 87, 1734, 1767
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HinfI GANTC 6 cut(s) 4, 132, 255, 301, 1275, 1894
HpaII CCGG 2 cut(s) 769, 1860
HphI GGTGA 1 cut(s) 497
Hpy166II GTNNAC 4 cut(s) 141, 394, 1190, 1590
Hpy188III TCNNGA 3 cut(s) 8, 221, 438
Hpy8I GTNNAC 4 cut(s) 141, 394, 1190, 1590
Hpy99I CGWCG 2 cut(s) 189, 1639
HpyAV CCTTC 3 cut(s) 17, 1379, 1559
HpyCH4III ACNGT 4 cut(s) 601, 984, 1168, 1429
HpyCH4IV ACGT 1 cut(s) 155
HpyCH4V TGCA 8 cut(s) 892, 973, 1053, 1171, 1397, 1439, 1619, 1731
HpyF10VI GCNNNNNNNGC 5 cut(s) 889, 1201, 1686, 1731, 1781
HpyF3I CTNAG 5 cut(s) 833, 1625, 1721, 1770, 1785
HpySE526I ACGT 1 cut(s) 155
Hsp92I GRCGYC 1 cut(s) 1586
HspAI GCGC 4 cut(s) 27, 87, 1734, 1767
KflI GGGWCCC 1 cut(s) 1606
Ksp22I TGATCA 2 cut(s) 480, 672
KspI CCGCGG 1 cut(s) 1673
Kzo9I GATC 5 cut(s) 480, 562, 672, 1068, 1863
LmnI GCTCC 5 cut(s) 772, 880, 1395, 1862, 1882
Lsp1109I GCAGC 7 cut(s) 449, 520, 1183, 1433, 1451, 1603, 1743
LweI GCATC 2 cut(s) 1253, 1406
MaeI CTAG 7 cut(s) 8, 438, 611, 956, 1160, 1373, 1448
MaeII ACGT 1 cut(s) 155
MaeIII GTNAC 4 cut(s) 118, 503, 632, 710
MalI GATC 5 cut(s) 482, 564, 674, 1070, 1865
MboI GATC 5 cut(s) 480, 562, 672, 1068, 1863
MboII GAAGA 2 cut(s) 322, 1832
MfeI CAATTG 1 cut(s) 1141
MflI RGATCY 2 cut(s) 562, 1863
MhlI GDGCHC 6 cut(s) 1150, 1206, 1220, 1392, 1643, 1859
MluI ACGCGT 1 cut(s) 1632
MlyI GAGTC 2 cut(s) 13, 141
MmeI TCCRAC 1 cut(s) 1893
Mph1103I ATGCAT 1 cut(s) 1399
MroXI GAANNNNTTC 1 cut(s) 1688
MseI TTAA 9 cut(s) 74, 290, 606, 644, 662, 903, 1031, 1041, 1797
MslI CAYNNNNRTG 4 cut(s) 719, 735, 1088, 1598
MspA1I CMGCKG 1 cut(s) 1672
MspI CCGG 2 cut(s) 769, 1860
MspR9I CCNGG 4 cut(s) 271, 770, 1583, 1861
MunI CAATTG 1 cut(s) 1141
MvaI CCWGG 2 cut(s) 271, 1583
MvnI CGCG 3 cut(s) 29, 1634, 1672
MwoI GCNNNNNNNGC 5 cut(s) 889, 1201, 1686, 1731, 1781
NciI CCSGG 2 cut(s) 770, 1861
NdeI CATATG 1 cut(s) 339
NdeII GATC 5 cut(s) 480, 562, 672, 1068, 1863
NlaIV GGNNCC 3 cut(s) 1383, 1607, 1608
NmuCI GTSAC 1 cut(s) 503
NsiI ATGCAT 1 cut(s) 1399
NspI RCATGY 2 cut(s) 1705, 1719
NspV TTCGAA 1 cut(s) 1656
PagI TCATGA 1 cut(s) 220
PciI ACATGT 1 cut(s) 1701
PdmI GAANNNNTTC 1 cut(s) 1688
PfeI GAWTC 4 cut(s) 255, 301, 1275, 1894
PflMI CCANNNNNTGG 1 cut(s) 1750
PfoI TCCNGGA 2 cut(s) 768, 1859
PkrI GCNGC 8 cut(s) 464, 535, 1173, 1423, 1441, 1618, 1674, 1733
PleI GAGTC 2 cut(s) 12, 140
PpsI GAGTC 2 cut(s) 12, 140
PpuMI RGGWCCY 1 cut(s) 1606
PscI ACATGT 1 cut(s) 1701
PshBI ATTAAT 2 cut(s) 290, 903
Psp124BI GAGCTC 1 cut(s) 1643
Psp1406I AACGTT 1 cut(s) 155
Psp5II RGGWCCY 1 cut(s) 1606
Psp6I CCWGG 2 cut(s) 269, 1581
PspGI CCWGG 2 cut(s) 269, 1581
PspN4I GGNNCC 3 cut(s) 1383, 1607, 1608
PspPI GGNCC 3 cut(s) 211, 1471, 1606
PspPPI RGGWCCY 1 cut(s) 1606
PstI CTGCAG 2 cut(s) 1441, 1733
PstNI CAGNNNCTG 1 cut(s) 1728
PsuI RGATCY 2 cut(s) 562, 1863
RsaI GTAC 2 cut(s) 1413, 1706
RsaNI GTAC 2 cut(s) 1412, 1705
RseI CAYNNNNRTG 4 cut(s) 719, 735, 1088, 1598
SacI GAGCTC 1 cut(s) 1643
SacII CCGCGG 1 cut(s) 1673
SaqAI TTAA 9 cut(s) 74, 290, 606, 644, 662, 903, 1031, 1041, 1797
SatI GCNGC 8 cut(s) 463, 534, 1172, 1422, 1440, 1617, 1673, 1732
Sau3AI GATC 5 cut(s) 480, 562, 672, 1068, 1863
Sau96I GGNCC 3 cut(s) 211, 1471, 1606
SchI GAGTC 2 cut(s) 13, 141
ScrFI CCNGG 4 cut(s) 271, 770, 1583, 1861
SduI GDGCHC 6 cut(s) 1150, 1206, 1220, 1392, 1643, 1859
SfaNI GCATC 2 cut(s) 1253, 1406
SfcI CTRYAG 2 cut(s) 1437, 1729
Sfr303I CCGCGG 1 cut(s) 1673
SfuI TTCGAA 1 cut(s) 1656
SgrBI CCGCGG 1 cut(s) 1673
SinI GGWCC 2 cut(s) 1471, 1606
SmiMI CAYNNNNRTG 4 cut(s) 719, 735, 1088, 1598
SmlI CTYRAG 2 cut(s) 991, 1556
SmoI CTYRAG 2 cut(s) 991, 1556
SpeI ACTAGT 1 cut(s) 955
SsiI CCGC 3 cut(s) 1670, 1672, 1687
SspI AATATT 3 cut(s) 417, 679, 901
SspMI CTAG 7 cut(s) 8, 438, 611, 956, 1160, 1373, 1448
SstI GAGCTC 1 cut(s) 1643
StyD4I CCNGG 4 cut(s) 269, 768, 1581, 1859
StyI CCWWGG 1 cut(s) 1749
TaaI ACNGT 4 cut(s) 601, 984, 1168, 1429
TaiI ACGT 1 cut(s) 158
TaqI TCGA 4 cut(s) 541, 1071, 1637, 1656
TatI WGTACW 1 cut(s) 1704
TauI GCSGC 1 cut(s) 1675
TfiI GAWTC 4 cut(s) 255, 301, 1275, 1894
Tru1I TTAA 9 cut(s) 74, 290, 606, 644, 662, 903, 1031, 1041, 1797
Tru9I TTAA 9 cut(s) 74, 290, 606, 644, 662, 903, 1031, 1041, 1797
TscAI CASTG 5 cut(s) 483, 987, 1095, 1173, 1434
TseFI GTSAC 1 cut(s) 503
TseI GCWGC 7 cut(s) 462, 533, 1171, 1421, 1439, 1616, 1731
Tsp45I GTSAC 1 cut(s) 503
TspRI CASTG 5 cut(s) 483, 987, 1095, 1173, 1434
Van91I CCANNNNNTGG 1 cut(s) 1750
VpaK11BI GGWCC 2 cut(s) 1471, 1606
VspI ATTAAT 2 cut(s) 290, 903
XagI CCTNNNNNAGG 1 cut(s) 557
XapI RAATTY 9 cut(s) 79, 92, 168, 441, 638, 666, 740, 1055, 1292
XbaI TCTAGA 2 cut(s) 7, 437
XceI RCATGY 2 cut(s) 1705, 1719
XcmI CCANNNNNNNNNTGG 1 cut(s) 1597
XmiI GTMKAC 1 cut(s) 140
XmnI GAANNNNTTC 1 cut(s) 1688
XspI CTAG 7 cut(s) 8, 438, 611, 956, 1160, 1373, 1448
Zsp2I ATGCAT 1 cut(s) 1399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.