pycom13g27920

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000158
Physical Location & Seq
Forward (+)
23983518 .. 23986256
2739 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g27920.14

Sequence Viewer

Length: 2004 bp
ATGACATTGTGTCACATCCCGGCCCGGGGGGACCACTTCCCGAGCCCGCTTCACCACCGTAACACGATATTGTCCGCTTTGGGCTTACTATTCCCTCACGGTTTTGTTTTTGGGAACTCACGAGCAACTTCTCAGTGGGTTACCCATCATGGGATTGCTCTAGCCCCCTTCTCGCTTAACTTCAGAGTTCCTATGGAACCCGAAGCCAGTAAGCTCCCAAAAGGCCTCGTGCTAGGTGGAGATGGGAATATACATTTAAGGATCACACCCCTGGGCGATGTGGGATGTCACAATCCACCCCCCTTAGGGTGTCACATTCCGGCCCGGGGGGACCACTTCCCGGGCCCGCTCCACCACCGTAGTACGATATGGTTCGCTTTGGGCTTACCATTCCCTCACGGTTTTGTTTTTGGGAACTCACGAGCAACTTCCCAGTGGGTCACCCATTATGGGATTGCTCTGGCCCCCTTCTCGCTTAACTTCGGAGTTCCTACGGAACCCGAAGCTAGTGAGCTCCCAAAAGGCCTCGCACTAGGTAGAGATGGGAATATACATTTAAGGATCACACCCCTGGACGATGTGGGATGTCACACATTGACCCACCAACACCTACCTCATGCAAAAATAATAAGTTCATCCCTACTATTATATTCAAAAGTGTCATACCATTATTTTCTCGCCTATAAGCTCTCAATATTTTATATAGGTTGCGAATTTAACTACCAGTCTACCCTCCAATGTGTATCACATGCAAGTAACGTGACTTATATTGACGAAAAATTGAATATAGTAGCTTCGAATATAATATTAGGGATGGACTTAATTTTTACTCATATGATAATTCATATACTAAATTGGTATATCTACAACCCTTCTCACAAGAGTGATAAGGAGGAGTTTCAAGTTATCATTCCAGAGAGTCATGGCATTGGCATCATCAATCTTGAGGTTCCCAGTCAGGAACTCCAGCTCCACATCACTCGCCTTTATCACATCAGCCTTATCCCATATGCTCATTATCTGCTTCCGTGCCTCATCTGCCGACGGCCACAGGGGCAGCCGCAGGTTTGGGTCGTAGGAGAGCAATGCACTTGTATCTTTAGCCACCTACACTGCCTTCAGATGTGCCGATCTGCACGACTCCATGATCAAGCTAATGGATCCATAGTGGAACACTCTGATTTCATTATCTATACTCATATAGTACCATGTAAAAAAGTTAAGCCTTCGAGATACATCAGGGCGTCCCATGTGCGCGCTAGGCTAGATTTGGATGCATTGTACATCTACTTGCATAAAAATGTAAATGTCAATTTAAAAAGAAAAAAGTGGGAAAATTATCACACTAGAAGAGGATTATCTCTGGATCCATCTTGTGGGGATCCTAGGAATCCCCATCTCCTAACCGTTCATTGTATATCGTGCGATAAGTTTTTGTTTGATACTATTTATATAAAGATCTCTAAGTCTTGTGATAGCCCGTCCCGGAATATTTTTATCAATGGTGTGAAAAGACTAAATTGCCTATCGACATTAGGTGGTGTTAACTTAGGTTTATTTTATTTTCTTTTCTTTTGGTTGGGTGCCATCTTTACCACACACACACACCCATACCACACTCCCGTGTACCCTCTCTCTCCTTCCCTTTTCAGATTTTCTTCCATTTCCGTACAACTATACAGTGATAGGAGCATATTTGTGCTACTTAGCTATGTTGTTTCCTTCTATTTTTGTGTGTTTGTAGGTCCTAATGGCAAAAGTAGCAAGAAAGTGCATTTTGGAGCTATTTGGAGGAGTTTTGGGCATGGAATAGATAGCTTGCATATGGAGCAAGGTAGATGGACGAAATTGAAGTCAAAAGAGGCTAGGAACATCAAGAAACATGGAACATTATCCAAACAACCTTATTTTATCCAAACCTTATCCAACCTTATCCCATCTTATCCTATCCTAATCATTTGCTACCTTAATTCTAGCTGCAAAGAGGACTTAATTTCACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

668

Amino Acids

75.53

Weight (kDa)

9.13

Isoelectric Point (pI)

49.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000158)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10163
malus_domestica MD04G1068000.v1.1 MD05G1130900.v1.1 MD06G1153600.v1.1 MD10G1255900.v1.1 MD10G1282000.v1.1 MD13G1075400.v1.1 MD13G1192400.v1.1
pyrus_communis pycom01g00130 pycom01g00390 pycom01g00700 pycom01g00720 pycom01g00750 pycom01g00850 pycom01g00870 pycom01g00910 pycom01g01250 pycom01g01560 pycom01g01680 pycom01g01860 pycom01g01900 pycom01g01910 pycom01g02200 pycom01g02260 pycom01g02270 pycom01g02280 pycom01g02330 pycom01g02480 pycom01g02720 pycom01g02730 pycom01g02750 pycom01g02770 pycom01g02780 pycom01g02860 pycom01g02910 pycom01g03540 pycom01g04090 pycom01g04100 pycom01g04150 pycom01g04250 pycom01g04430 pycom01g11810 pycom01g11860 pycom01g11940 pycom01g21380 pycom02g06650 pycom02g08490 pycom02g08600 pycom02g18230 pycom02g18450 pycom02g18800 pycom02g19150 pycom02g20740 pycom02g21340 pycom02g26210 pycom03g12060 pycom03g19060 pycom03g19100 pycom03g19120 pycom04g06890 pycom04g08960 pycom05g01790 pycom05g07910 pycom05g08080 pycom05g08100 pycom05g08570 pycom05g21500 pycom05g22400 pycom05g22510 pycom05g22620 pycom05g23020 pycom05g23720 pycom05g23890 pycom05g24040 pycom07g12080 pycom08g07930 pycom08g11940 pycom09g12120 pycom10g03890 pycom10g23360 pycom10g28250 pycom11g02850 pycom11g14370 pycom11g14400 pycom11g21840 pycom11g21860 pycom11g21980 pycom11g23580 pycom11g23650 pycom11g24050 pycom11g25610 pycom12424g00060 pycom1256g00130 pycom12661g00030 pycom12g09520 pycom12g09530 pycom12g09660 pycom12g09730 pycom12g11210 pycom12g16710 pycom12g16740 pycom12g16750 pycom13g08800 pycom13g18580 pycom13g18670 pycom13g18770 pycom13g22900 pycom13g23050 pycom13g23160 pycom13g23480 pycom13g23610 pycom13g23660 pycom13g23790 pycom13g23840 pycom13g24010 pycom13g24140 pycom13g24210 pycom13g24300 pycom13g24310 pycom13g24480 pycom13g24520 pycom13g24570 pycom13g24730 pycom13g24760 pycom13g24820 pycom13g25010 pycom13g25020 pycom13g25270 pycom13g25620 pycom13g25670 pycom13g25760 pycom13g25800 pycom13g25900 pycom13g26620 pycom13g26640 pycom13g26670 pycom13g26940 pycom13g27040 pycom13g27130 pycom13g27530 pycom13g27770 pycom13g27830 pycom13g27920 pycom13g27940 pycom13g28100 pycom13g28420 pycom13g28430 pycom13g28460 pycom13g28520 pycom13g29160 pycom14g05380 pycom15g03480 pycom15g04370 pycom15g27500 pycom16g13590 pycom16g13610 pycom16g13630 pycom16g14390 pycom16g18310 pycom17g01940 pycom17g04000 pycom17g16100 pycom17g16220 pycom17g16230 pycom17g16340 pycom17g16430 pycom17g16460 pycom17g16740 pycom17g16960 pycom17g17020 pycom17g17090 pycom17g17100 pycom17g17170 pycom17g17430 pycom17g17850 pycom17g18230 pycom17g18250 pycom17g18380 pycom17g21170 pycom17g21330 pycom520g00150 pycom520g00160 pycom808g00030 pycom808g00160 pycom808g00250 pycom808g00290
rosa_chinensis RchiOBHm_Chr5g0054451

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1054
AccB1I GGYRCC 1 cut(s) 1583
AccB7I CCANNNNNTGG 1 cut(s) 1376
AccBSI CCGCTC 1 cut(s) 349
AccI GTMKAC 1 cut(s) 728
AccII CGCG 1 cut(s) 1257
AciI CCGC 4 cut(s) 47, 75, 347, 1061
AclWI GGATC 8 cut(s) 269, 569, 1155, 1168, 1361, 1374, 1376, 1389
AcoI YGGCCR 1 cut(s) 1046
AcsI RAATTY 1 cut(s) 713
AcuI CTGAAG 2 cut(s) 166, 1103
AcyI GRCGYC 1 cut(s) 1244
AfaI GTAC 5 cut(s) 364, 1206, 1283, 1628, 1671
AfiI CCNNNNNNNGG 9 cut(s) 25, 26, 150, 305, 306, 326, 340, 450, 1376
AgsI TTSAA 4 cut(s) 654, 784, 902, 1852
AhdI GACNNNNNGTC 1 cut(s) 9
AjnI CCWGG 2 cut(s) 270, 570
AleI CACNNNNGTG 2 cut(s) 882, 1622
AloI GAACNNNNNNTCC 2 cut(s) 954, 986
Alw21I GWGCWC 1 cut(s) 516
AlwI GGATC 8 cut(s) 269, 569, 1155, 1168, 1361, 1374, 1376, 1389
Ama87I CYCGRG 4 cut(s) 24, 40, 324, 340
AoxI GGCC 7 cut(s) 21, 223, 321, 343, 462, 523, 1046
ApaI GGGCCC 1 cut(s) 347
ApeKI GCWGC 2 cut(s) 1057, 1977
ApoI RAATTY 1 cut(s) 713
ArsI GACNNNNNNTTYG 2 cut(s) 1838, 1870
AspA2I CCTAGG 1 cut(s) 1385
AspLEI GCGC 2 cut(s) 1257, 1259
AspS9I GGNCC 8 cut(s) 22, 31, 322, 331, 343, 344, 463, 1745
AsuC2I CCSGG 8 cut(s) 20, 25, 26, 325, 326, 341, 342, 1486
AsuHPI GGTGA 2 cut(s) 44, 433
AsuII TTCGAA 1 cut(s) 797
AvaI CYCGRG 4 cut(s) 24, 40, 324, 340
AvaII GGWCC 3 cut(s) 31, 331, 1745
AvrII CCTAGG 1 cut(s) 1385
AxyI CCTNAGG 1 cut(s) 304
BaeGI GKGCMC 1 cut(s) 347
BamHI GGATCC 3 cut(s) 1160, 1366, 1381
BanI GGYRCC 1 cut(s) 1583
BanII GRGCYC 3 cut(s) 47, 347, 516
BauI CACGAG 3 cut(s) 120, 227, 420
Bbv12I GWGCWC 1 cut(s) 516
BbvI GCAGC 2 cut(s) 1069, 1964
BccI CCATC 9 cut(s) 153, 236, 536, 808, 1378, 1404, 1595, 1833, 1945
BceAI ACGGC 1 cut(s) 1061
BciT130I CCWGG 2 cut(s) 272, 572
BclI TGATCA 1 cut(s) 1147
BcnI CCSGG 8 cut(s) 20, 25, 26, 325, 326, 341, 342, 1486
BfuAI ACCTGC 1 cut(s) 1054
BglI GCCNNNNNGGC 1 cut(s) 1054
BglII AGATCT 1 cut(s) 1458
BisI GCNGC 3 cut(s) 1058, 1061, 1978
BlnI CCTAGG 1 cut(s) 1385
BlsI GCNGC 3 cut(s) 1059, 1062, 1979
Bme18I GGWCC 3 cut(s) 31, 331, 1745
BmeRI GACNNNNNGTC 1 cut(s) 9
BmeT110I CYCGRG 4 cut(s) 24, 40, 324, 340
BmgT120I GGNCC 8 cut(s) 22, 31, 322, 331, 343, 344, 463, 1745
BmrI ACTGGG 2 cut(s) 427, 948
BmsI GCATC 2 cut(s) 942, 1264
BmuI ACTGGG 2 cut(s) 427, 948
BpmI CTGGAG 1 cut(s) 950
Bpu14I TTCGAA 1 cut(s) 797
BpuEI CTTGAG 1 cut(s) 965
BpuMI CCSGG 8 cut(s) 20, 25, 26, 325, 326, 341, 342, 1486
BsaHI GRCGYC 1 cut(s) 1244
BsaJI CCNNGG 9 cut(s) 24, 25, 270, 271, 324, 325, 340, 570, 1385
BsaXI ACNNNNNCTCC 2 cut(s) 954, 984
Bsc4I CCNNNNNNNGG 9 cut(s) 25, 26, 150, 305, 306, 326, 340, 450, 1376
Bse1I ACTGG 4 cut(s) 207, 433, 724, 954
Bse21I CCTNAGG 1 cut(s) 304
Bse3DI GCAATG 1 cut(s) 1091
BseBI CCWGG 2 cut(s) 272, 572
BseDI CCNNGG 9 cut(s) 24, 25, 270, 271, 324, 325, 340, 570, 1385
BseGI GGATG 6 cut(s) 15, 290, 590, 635, 819, 1279
BseLI CCNNNNNNNGG 9 cut(s) 25, 26, 150, 305, 306, 326, 340, 450, 1376
BseMI GCAATG 1 cut(s) 1091
BseMII CTCAG 1 cut(s) 146
BseNI ACTGG 4 cut(s) 207, 433, 724, 954
BsePI GCGCGC 1 cut(s) 1255
BseRI GAGGAG 2 cut(s) 908, 1807
BseSI GKGCMC 1 cut(s) 347
BseXI GCAGC 2 cut(s) 1069, 1964
BsgI GTGCAG 1 cut(s) 1119
Bsh1236I CGCG 1 cut(s) 1257
BshFI GGCC 7 cut(s) 23, 225, 323, 345, 464, 525, 1048
BshNI GGYRCC 1 cut(s) 1583
BsiHKAI GWGCWC 1 cut(s) 516
BsiHKCI CYCGRG 4 cut(s) 24, 40, 324, 340
BsiSI CCGG 6 cut(s) 20, 25, 320, 325, 341, 1486
BslFI GGGAC 4 cut(s) 44, 344, 1231, 1468
BslI CCNNNNNNNGG 9 cut(s) 25, 26, 150, 305, 306, 326, 340, 450, 1376
BsmFI GGGAC 4 cut(s) 44, 344, 1231, 1468
BsnI GGCC 7 cut(s) 23, 225, 323, 345, 464, 525, 1048
BsoBI CYCGRG 4 cut(s) 24, 40, 324, 340
Bsp119I TTCGAA 1 cut(s) 797
Bsp120I GGGCCC 1 cut(s) 343
Bsp1286I GDGCHC 3 cut(s) 47, 347, 516
Bsp1407I TGTACA 1 cut(s) 1281
Bsp143I GATC 8 cut(s) 261, 561, 1130, 1147, 1160, 1366, 1381, 1458
BspACI CCGC 4 cut(s) 47, 75, 347, 1061
BspANI GGCC 7 cut(s) 23, 225, 323, 345, 464, 525, 1048
BspCNI CTCAG 1 cut(s) 145
BspFNI CGCG 1 cut(s) 1257
BspMI ACCTGC 1 cut(s) 1054
BspPI GGATC 8 cut(s) 269, 569, 1155, 1168, 1361, 1374, 1376, 1389
BspT104I TTCGAA 1 cut(s) 797
BspT107I GGYRCC 1 cut(s) 1583
BsrBI CCGCTC 1 cut(s) 349
BsrDI GCAATG 1 cut(s) 1091
BsrGI TGTACA 1 cut(s) 1281
BsrI ACTGG 4 cut(s) 207, 433, 724, 954
BssECI CCNNGG 9 cut(s) 24, 25, 270, 271, 324, 325, 340, 570, 1385
BssHII GCGCGC 1 cut(s) 1255
BssMI GATC 8 cut(s) 261, 561, 1130, 1147, 1160, 1366, 1381, 1458
BssNI GRCGYC 1 cut(s) 1244
BssSI CACGAG 3 cut(s) 120, 227, 420
BssT1I CCWWGG 1 cut(s) 1385
Bst2BI CACGAG 3 cut(s) 120, 227, 420
Bst2UI CCWGG 2 cut(s) 272, 572
Bst4CI ACNGT 6 cut(s) 59, 101, 359, 401, 1408, 1682
Bst6I CTCTTC 1 cut(s) 1345
BstACI GRCGYC 1 cut(s) 1244
BstAUI TGTACA 1 cut(s) 1281
BstBI TTCGAA 1 cut(s) 797
BstC8I GCNNGC 4 cut(s) 47, 347, 1257, 1820
BstDEI CTNAG 5 cut(s) 132, 304, 1464, 1549, 1706
BstEII GGTNACC 2 cut(s) 139, 439
BstF5I GGATG 6 cut(s) 15, 290, 590, 635, 819, 1279
BstFNI CGCG 1 cut(s) 1257
BstHHI GCGC 2 cut(s) 1257, 1259
BstKTI GATC 8 cut(s) 264, 564, 1133, 1150, 1163, 1369, 1384, 1461
BstMBI GATC 8 cut(s) 261, 561, 1130, 1147, 1160, 1366, 1381, 1458
BstMWI GCNNNNNNNGC 5 cut(s) 1038, 1054, 1261, 1761, 1828
BstNI CCWGG 2 cut(s) 272, 572
BstNSI RCATGY 1 cut(s) 752
BstPI GGTNACC 2 cut(s) 139, 439
BstSLI GKGCMC 1 cut(s) 347
BstUI CGCG 1 cut(s) 1257
BstV1I GCAGC 2 cut(s) 1069, 1964
BstX2I RGATCY 4 cut(s) 1160, 1366, 1381, 1458
BstYI RGATCY 4 cut(s) 1160, 1366, 1381, 1458
Bsu36I CCTNAGG 1 cut(s) 304
BsuRI GGCC 7 cut(s) 23, 225, 323, 345, 464, 525, 1048
BtgZI GCGATG 1 cut(s) 291
BtsCI GGATG 6 cut(s) 15, 290, 590, 635, 819, 1279
BtsI GCAGTG 1 cut(s) 1111
BtsIMutI CAGTG 4 cut(s) 140, 440, 1111, 1687
BveI ACCTGC 1 cut(s) 1054
Cac8I GCNNGC 4 cut(s) 47, 347, 1257, 1820
CfoI GCGC 2 cut(s) 1257, 1259
Cfr13I GGNCC 8 cut(s) 22, 31, 322, 331, 343, 344, 463, 1745
Cfr9I CCCGGG 3 cut(s) 24, 324, 340
CseI GACGC 1 cut(s) 1233
Csp6I GTAC 5 cut(s) 363, 1205, 1282, 1627, 1670
CviAII CATG 9 cut(s) 149, 617, 749, 923, 1145, 1209, 1250, 1805, 1883
CviQI GTAC 5 cut(s) 363, 1205, 1282, 1627, 1670
DdeI CTNAG 5 cut(s) 132, 304, 1464, 1549, 1706
DpnI GATC 8 cut(s) 263, 563, 1132, 1149, 1162, 1368, 1383, 1460
DpnII GATC 8 cut(s) 261, 561, 1130, 1147, 1160, 1366, 1381, 1458
DraI TTTAAA 1 cut(s) 1317
DriI GACNNNNNGTC 1 cut(s) 9
EaeI YGGCCR 1 cut(s) 1046
Eam1104I CTCTTC 1 cut(s) 1345
Eam1105I GACNNNNNGTC 1 cut(s) 9
EarI CTCTTC 1 cut(s) 1345
Ecl136II GAGCTC 1 cut(s) 514
Eco130I CCWWGG 1 cut(s) 1385
Eco147I AGGCCT 2 cut(s) 225, 525
Eco24I GRGCYC 3 cut(s) 47, 347, 516
Eco47I GGWCC 3 cut(s) 31, 331, 1745
Eco53kI GAGCTC 1 cut(s) 514
Eco57I CTGAAG 2 cut(s) 166, 1103
Eco81I CCTNAGG 1 cut(s) 304
Eco88I CYCGRG 4 cut(s) 24, 40, 324, 340
Eco91I GGTNACC 2 cut(s) 139, 439
EcoICRI GAGCTC 1 cut(s) 514
EcoO109I RGGNCCY 1 cut(s) 1745
EcoO65I GGTNACC 2 cut(s) 139, 439
EcoRII CCWGG 2 cut(s) 270, 570
EcoT14I CCWWGG 1 cut(s) 1385
EcoT22I ATGCAT 1 cut(s) 1279
EcoT38I GRGCYC 3 cut(s) 47, 347, 516
ErhI CCWWGG 1 cut(s) 1385
FaeI CATG 9 cut(s) 152, 620, 752, 926, 1148, 1212, 1253, 1808, 1886
FaqI GGGAC 4 cut(s) 44, 344, 1231, 1468
FatI CATG 9 cut(s) 148, 616, 748, 922, 1144, 1208, 1249, 1804, 1882
FauI CCCGC 2 cut(s) 54, 354
FauNDI CATATG 3 cut(s) 834, 1009, 1824
FbaI TGATCA 1 cut(s) 1147
FblI GTMKAC 1 cut(s) 728
Fnu4HI GCNGC 3 cut(s) 1058, 1061, 1978
FokI GGATG 6 cut(s) 2, 297, 597, 622, 826, 1286
FriOI GRGCYC 3 cut(s) 47, 347, 516
Fsp4HI GCNGC 3 cut(s) 1058, 1061, 1978
GlaI GCGC 2 cut(s) 1256, 1258
GluI GCNGC 3 cut(s) 1058, 1061, 1978
GsuI CTGGAG 1 cut(s) 950
HaeIII GGCC 7 cut(s) 23, 225, 323, 345, 464, 525, 1048
HapII CCGG 6 cut(s) 20, 25, 320, 325, 341, 1486
HgaI GACGC 1 cut(s) 1233
HhaI GCGC 2 cut(s) 1257, 1259
Hin1I GRCGYC 1 cut(s) 1244
Hin1II CATG 9 cut(s) 152, 620, 752, 926, 1148, 1212, 1253, 1808, 1886
Hin6I GCGC 2 cut(s) 1255, 1257
HinP1I GCGC 2 cut(s) 1255, 1257
HincII GTYRAC 1 cut(s) 1546
HindII GTYRAC 1 cut(s) 1546
HinfI GANTC 3 cut(s) 919, 1140, 1390
HpaI GTTAAC 1 cut(s) 1546
HpaII CCGG 6 cut(s) 20, 25, 320, 325, 341, 1486
HphI GGTGA 2 cut(s) 44, 433
Hpy166II GTNNAC 3 cut(s) 729, 1546, 1627
Hpy188I TCNGA 5 cut(s) 185, 485, 1122, 1180, 1652
Hpy188III TCNNGA 9 cut(s) 40, 120, 420, 914, 944, 959, 1230, 1364, 1876
Hpy8I GTNNAC 3 cut(s) 729, 1546, 1627
Hpy99I CGWCG 1 cut(s) 1047
HpyAV CCTTC 7 cut(s) 178, 478, 882, 1127, 1236, 1650, 1732
HpyCH4III ACNGT 6 cut(s) 59, 101, 359, 401, 1408, 1682
HpyCH4IV ACGT 1 cut(s) 759
HpyCH4V TGCA 9 cut(s) 620, 752, 1089, 1136, 1277, 1294, 1774, 1822, 1980
HpyF10VI GCNNNNNNNGC 5 cut(s) 1038, 1054, 1261, 1761, 1828
HpyF3I CTNAG 5 cut(s) 132, 304, 1464, 1549, 1706
HpySE526I ACGT 1 cut(s) 759
Hsp92I GRCGYC 1 cut(s) 1244
Hsp92II CATG 9 cut(s) 152, 620, 752, 926, 1148, 1212, 1253, 1808, 1886
HspAI GCGC 2 cut(s) 1255, 1257
Ksp22I TGATCA 1 cut(s) 1147
KspAI GTTAAC 1 cut(s) 1546
Kzo9I GATC 8 cut(s) 261, 561, 1130, 1147, 1160, 1366, 1381, 1458
LmnI GCTCC 7 cut(s) 219, 354, 519, 975, 1689, 1781, 1828
Lsp1109I GCAGC 2 cut(s) 1069, 1964
LweI GCATC 2 cut(s) 942, 1264
MaeII ACGT 1 cut(s) 759
MaeIII GTNAC 9 cut(s) 11, 59, 139, 287, 311, 439, 587, 755, 760
MalI GATC 8 cut(s) 263, 563, 1132, 1149, 1162, 1368, 1383, 1460
MbiI CCGCTC 1 cut(s) 349
MboI GATC 8 cut(s) 261, 561, 1130, 1147, 1160, 1366, 1381, 1458
MboII GAAGA 2 cut(s) 1362, 1650
MflI RGATCY 4 cut(s) 1160, 1366, 1381, 1458
MhlI GDGCHC 3 cut(s) 47, 347, 516
MlyI GAGTC 2 cut(s) 928, 1134
MmeI TCCRAC 1 cut(s) 1950
Mph1103I ATGCAT 1 cut(s) 1279
MslI CAYNNNNRTG 4 cut(s) 882, 1299, 1622, 1697
MspI CCGG 6 cut(s) 20, 25, 320, 325, 341, 1486
MvaI CCWGG 2 cut(s) 272, 572
MvnI CGCG 1 cut(s) 1257
MwoI GCNNNNNNNGC 5 cut(s) 1038, 1054, 1261, 1761, 1828
NciI CCSGG 8 cut(s) 20, 25, 26, 325, 326, 341, 342, 1486
NdeI CATATG 3 cut(s) 834, 1009, 1824
NdeII GATC 8 cut(s) 261, 561, 1130, 1147, 1160, 1366, 1381, 1458
NlaIII CATG 9 cut(s) 152, 620, 752, 926, 1148, 1212, 1253, 1808, 1886
NmuCI GTSAC 6 cut(s) 11, 287, 311, 439, 587, 760
NsiI ATGCAT 1 cut(s) 1279
NspI RCATGY 1 cut(s) 752
NspV TTCGAA 1 cut(s) 797
OliI CACNNNNGTG 2 cut(s) 882, 1622
PasI CCCWGGG 1 cut(s) 271
PauI GCGCGC 1 cut(s) 1255
PceI AGGCCT 2 cut(s) 225, 525
PfeI GAWTC 1 cut(s) 1390
PflMI CCANNNNNTGG 1 cut(s) 1376
PfoI TCCNGGA 1 cut(s) 1484
PkrI GCNGC 3 cut(s) 1059, 1062, 1979
PleI GAGTC 2 cut(s) 927, 1134
PpsI GAGTC 2 cut(s) 927, 1134
PpuMI RGGWCCY 1 cut(s) 1745
Psp124BI GAGCTC 1 cut(s) 516
Psp5II RGGWCCY 1 cut(s) 1745
Psp6I CCWGG 2 cut(s) 270, 570
PspEI GGTNACC 2 cut(s) 139, 439
PspGI CCWGG 2 cut(s) 270, 570
PspOMI GGGCCC 1 cut(s) 343
PspPI GGNCC 8 cut(s) 22, 31, 322, 331, 343, 344, 463, 1745
PspPPI RGGWCCY 1 cut(s) 1745
PsuI RGATCY 4 cut(s) 1160, 1366, 1381, 1458
PteI GCGCGC 1 cut(s) 1255
RsaI GTAC 5 cut(s) 364, 1206, 1283, 1628, 1671
RsaNI GTAC 5 cut(s) 363, 1205, 1282, 1627, 1670
RseI CAYNNNNRTG 4 cut(s) 882, 1299, 1622, 1697
SacI GAGCTC 1 cut(s) 516
SatI GCNGC 3 cut(s) 1058, 1061, 1978
Sau3AI GATC 8 cut(s) 261, 561, 1130, 1147, 1160, 1366, 1381, 1458
Sau96I GGNCC 8 cut(s) 22, 31, 322, 331, 343, 344, 463, 1745
SchI GAGTC 2 cut(s) 928, 1134
SduI GDGCHC 3 cut(s) 47, 347, 516
SfaNI GCATC 2 cut(s) 942, 1264
SfuI TTCGAA 1 cut(s) 797
SinI GGWCC 3 cut(s) 31, 331, 1745
SmaI CCCGGG 3 cut(s) 26, 326, 342
SmiMI CAYNNNNRTG 4 cut(s) 882, 1299, 1622, 1697
SmlI CTYRAG 1 cut(s) 944
SmoI CTYRAG 1 cut(s) 944
SseBI AGGCCT 2 cut(s) 225, 525
SsiI CCGC 4 cut(s) 47, 75, 347, 1061
SspI AATATT 3 cut(s) 696, 807, 1492
SstI GAGCTC 1 cut(s) 516
StuI AGGCCT 2 cut(s) 225, 525
StyI CCWWGG 1 cut(s) 1385
TaaI ACNGT 6 cut(s) 59, 101, 359, 401, 1408, 1682
TaiI ACGT 1 cut(s) 762
TaqI TCGA 3 cut(s) 797, 1229, 1529
TatI WGTACW 1 cut(s) 1281
TauI GCSGC 1 cut(s) 1063
TfiI GAWTC 1 cut(s) 1390
TscAI CASTG 4 cut(s) 140, 440, 1118, 1687
TseFI GTSAC 6 cut(s) 11, 287, 311, 439, 587, 760
TseI GCWGC 2 cut(s) 1057, 1977
Tsp45I GTSAC 6 cut(s) 11, 287, 311, 439, 587, 760
TspDTI ATGAA 4 cut(s) 624, 833, 1174, 1400
TspGWI ACGGA 3 cut(s) 509, 1017, 1657
TspMI CCCGGG 3 cut(s) 24, 324, 340
TspRI CASTG 4 cut(s) 140, 440, 1118, 1687
Van91I CCANNNNNTGG 1 cut(s) 1376
VpaK11BI GGWCC 3 cut(s) 31, 331, 1745
XapI RAATTY 1 cut(s) 713
XceI RCATGY 1 cut(s) 752
XmaI CCCGGG 3 cut(s) 24, 324, 340
XmaJI CCTAGG 1 cut(s) 1385
XmiI GTMKAC 1 cut(s) 728
Zsp2I ATGCAT 1 cut(s) 1279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.