pycom01g01900

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000158
Physical Location & Seq
Forward (+)
1681002 .. 1682712
1711 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g01900.10

Sequence Viewer

Length: 1194 bp
ATGTTATTGGTATTCCAAAAATCTCATTTTATACTCCTGACAAGTGTATTTTTCTTTTTAAATATAGAAAGTTTGGAGTGTAGAATGAGAATTTTGGATTTATTAGACTATTTCCAACCCTTTGATCTTGACCCGAGATTATTAAAGAATGAATTTAGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTCTGAAGTAGGTTCTTTTTTAAAAATAAAAAATAAAAATTATGTCACATCCCGCCCCGGGACGGATCACTTCCCGGGCCCGCTCCACCACTGTAGCATGATATTGTCCGCTTTAGACTTATCATTCCCTCACGGTTTTGTTTTTGGAAACTCACGAGCAACTTCCCAGTGGGTCACCCATCATGGGATTGCTCTAGCCCCCTTCTCGCTTAACTTCAGAGTTCCTATGGAACCTGAAGCCAATGAGCTCCCAAAAGGCCTCGTGCAAGGTAGGGATGAGAATATACATTTAAGGATCACTCCCCTGGGTGATGTGGGATGTTACAATCCACCCCCCTTAGGGGCCCGACATCCTCGTCAGCACACCACGGCCAAGCACGTTATTGTCCGCTTTGGGCTTACCATTCCCTCACGGTTTTGTTTTTGGGAACTCACAAGCAACTTCCCATTGGGTCACCCATTATGGAATTGCTCTAGCCCCCCCTTCTCGCTTAACTTCAGAGTTCCTACGGAACCCGAAGCCAGTGAGCTCCCAAAAGGCGTCGTGCTAGGTAGGGATGAGAATATACATTTAAGGATCACTCCCCTGGGCGATGTGGGATATTTTTTAATTAATTTAGCTGTTGGATTTAATTTTGGACAATTAGAACTTTTTTTTTACCTAAATAAATTAAATGTGGACCGTTGGATAACCAATGGTCTGTCACAAGGCGAGATGATGGCTGACACACGAGTGGGCCAGCAAAATGCTAAGGCTACTCAACACATGCGGGCCTGCTCTCTTGCTTTTTTGAAGCCAGAAGCACGTCTAATTTTACTTTTAGTTATTTTATTAACACTTCCAAACAAATCCTTAGATTTGGATATTCGGTGCAAGTACTTACATGACAACTACCAAATTAACGACATCGATCTCTGCTCGTCTTTTTATAATTACAAACGATATCATTATTTAATATGCACCAGAAAAAATAAAAGGAAGAATTTAAACTCAAATACAATGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

398

Amino Acids

45.79

Weight (kDa)

9.0

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000158)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10163
malus_domestica MD04G1068000.v1.1 MD05G1130900.v1.1 MD06G1153600.v1.1 MD10G1255900.v1.1 MD10G1282000.v1.1 MD13G1075400.v1.1 MD13G1192400.v1.1
pyrus_communis pycom01g00130 pycom01g00390 pycom01g00700 pycom01g00720 pycom01g00750 pycom01g00850 pycom01g00870 pycom01g00910 pycom01g01250 pycom01g01560 pycom01g01680 pycom01g01860 pycom01g01900 pycom01g01910 pycom01g02200 pycom01g02260 pycom01g02270 pycom01g02280 pycom01g02330 pycom01g02480 pycom01g02720 pycom01g02730 pycom01g02750 pycom01g02770 pycom01g02780 pycom01g02860 pycom01g02910 pycom01g03540 pycom01g04090 pycom01g04100 pycom01g04150 pycom01g04250 pycom01g04430 pycom01g11810 pycom01g11860 pycom01g11940 pycom01g21380 pycom02g06650 pycom02g08490 pycom02g08600 pycom02g18230 pycom02g18450 pycom02g18800 pycom02g19150 pycom02g20740 pycom02g21340 pycom02g26210 pycom03g12060 pycom03g19060 pycom03g19100 pycom03g19120 pycom04g06890 pycom04g08960 pycom05g01790 pycom05g07910 pycom05g08080 pycom05g08100 pycom05g08570 pycom05g21500 pycom05g22400 pycom05g22510 pycom05g22620 pycom05g23020 pycom05g23720 pycom05g23890 pycom05g24040 pycom07g12080 pycom08g07930 pycom08g11940 pycom09g12120 pycom10g03890 pycom10g23360 pycom10g28250 pycom11g02850 pycom11g14370 pycom11g14400 pycom11g21840 pycom11g21860 pycom11g21980 pycom11g23580 pycom11g23650 pycom11g24050 pycom11g25610 pycom12424g00060 pycom1256g00130 pycom12661g00030 pycom12g09520 pycom12g09530 pycom12g09660 pycom12g09730 pycom12g11210 pycom12g16710 pycom12g16740 pycom12g16750 pycom13g08800 pycom13g18580 pycom13g18670 pycom13g18770 pycom13g22900 pycom13g23050 pycom13g23160 pycom13g23480 pycom13g23610 pycom13g23660 pycom13g23790 pycom13g23840 pycom13g24010 pycom13g24140 pycom13g24210 pycom13g24300 pycom13g24310 pycom13g24480 pycom13g24520 pycom13g24570 pycom13g24730 pycom13g24760 pycom13g24820 pycom13g25010 pycom13g25020 pycom13g25270 pycom13g25620 pycom13g25670 pycom13g25760 pycom13g25800 pycom13g25900 pycom13g26620 pycom13g26640 pycom13g26670 pycom13g26940 pycom13g27040 pycom13g27130 pycom13g27530 pycom13g27770 pycom13g27830 pycom13g27920 pycom13g27940 pycom13g28100 pycom13g28420 pycom13g28430 pycom13g28460 pycom13g28520 pycom13g29160 pycom14g05380 pycom15g03480 pycom15g04370 pycom15g27500 pycom16g13590 pycom16g13610 pycom16g13630 pycom16g14390 pycom16g18310 pycom17g01940 pycom17g04000 pycom17g16100 pycom17g16220 pycom17g16230 pycom17g16340 pycom17g16430 pycom17g16460 pycom17g16740 pycom17g16960 pycom17g17020 pycom17g17090 pycom17g17100 pycom17g17170 pycom17g17430 pycom17g17850 pycom17g18230 pycom17g18250 pycom17g18380 pycom17g21170 pycom17g21330 pycom520g00150 pycom520g00160 pycom808g00030 pycom808g00160 pycom808g00250 pycom808g00290
rosa_chinensis RchiOBHm_Chr5g0054451

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1116
AasI GACNNNNNNGTC 1 cut(s) 540
AccBSI CCGCTC 1 cut(s) 268
AciI CCGC 5 cut(s) 238, 266, 294, 574, 955
AclWI GGATC 3 cut(s) 258, 488, 770
AcoI YGGCCR 1 cut(s) 555
AcsI RAATTY 3 cut(s) 90, 152, 1168
AcuI CTGAAG 4 cut(s) 210, 385, 441, 667
AcyI GRCGYC 1 cut(s) 726
AfaI GTAC 1 cut(s) 1064
AfiI CCNNNNNNNGG 6 cut(s) 243, 247, 369, 524, 525, 526
AgsI TTSAA 1 cut(s) 979
AjiI CACGTC 1 cut(s) 992
AjnI CCWGG 2 cut(s) 489, 771
AleI CACNNNNGTG 1 cut(s) 917
AluBI AGCT 3 cut(s) 433, 715, 806
AluI AGCT 3 cut(s) 433, 715, 806
Alw21I GWGCWC 2 cut(s) 435, 717
AlwI GGATC 3 cut(s) 258, 488, 770
Ama87I CYCGRG 3 cut(s) 133, 242, 259
AoxI GGCC 6 cut(s) 262, 442, 528, 555, 922, 957
ApaI GGGCCC 2 cut(s) 266, 532
ApoI RAATTY 3 cut(s) 90, 152, 1168
AseI ATTAAT 1 cut(s) 798
AspS9I GGNCC 7 cut(s) 262, 263, 528, 529, 865, 922, 957
AsuC2I CCSGG 4 cut(s) 243, 244, 260, 261
AsuHPI GGTGA 3 cut(s) 352, 506, 632
AvaI CYCGRG 3 cut(s) 133, 242, 259
AvaII GGWCC 1 cut(s) 865
AxyI CCTNAGG 1 cut(s) 523
BaeGI GKGCMC 2 cut(s) 266, 532
BanII GRGCYC 4 cut(s) 266, 435, 532, 717
BauI CACGAG 3 cut(s) 339, 446, 915
Bbv12I GWGCWC 2 cut(s) 435, 717
BccI CCATC 2 cut(s) 372, 898
BceAI ACGGC 1 cut(s) 570
BciT130I CCWGG 2 cut(s) 491, 773
BcnI CCSGG 4 cut(s) 243, 244, 260, 261
BfaI CTAG 3 cut(s) 380, 660, 734
BfmI CTRYAG 1 cut(s) 277
BmcAI AGTACT 1 cut(s) 1064
Bme1390I CCNGG 6 cut(s) 243, 244, 260, 261, 491, 773
Bme18I GGWCC 1 cut(s) 865
BmeT110I CYCGRG 3 cut(s) 133, 242, 259
BmgBI CACGTC 1 cut(s) 992
BmgT120I GGNCC 7 cut(s) 262, 263, 528, 529, 865, 922, 957
BmiI GGNNCC 5 cut(s) 264, 417, 529, 530, 699
BmrFI CCNGG 6 cut(s) 243, 244, 260, 261, 491, 773
BmrI ACTGGG 1 cut(s) 346
BmuI ACTGGG 1 cut(s) 346
Bpu10I CCTNAGC 1 cut(s) 936
BpuMI CCSGG 4 cut(s) 243, 244, 260, 261
Bsa29I ATCGAT 1 cut(s) 1095
BsaHI GRCGYC 1 cut(s) 726
BsaJI CCNNGG 8 cut(s) 241, 242, 259, 489, 490, 552, 771, 772
Bsc4I CCNNNNNNNGG 6 cut(s) 243, 247, 369, 524, 525, 526
Bse1I ACTGG 2 cut(s) 352, 708
Bse21I CCTNAGG 1 cut(s) 523
BseBI CCWGG 2 cut(s) 491, 773
BseCI ATCGAT 1 cut(s) 1095
BseDI CCNNGG 8 cut(s) 241, 242, 259, 489, 490, 552, 771, 772
BseGI GGATG 5 cut(s) 233, 466, 509, 535, 748
BseLI CCNNNNNNNGG 6 cut(s) 243, 247, 369, 524, 525, 526
BseNI ACTGG 2 cut(s) 352, 708
BseSI GKGCMC 2 cut(s) 266, 532
BshFI GGCC 6 cut(s) 264, 444, 530, 557, 924, 959
BshVI ATCGAT 1 cut(s) 1095
BsiHKAI GWGCWC 2 cut(s) 435, 717
BsiHKCI CYCGRG 3 cut(s) 133, 242, 259
BsiSI CCGG 2 cut(s) 243, 260
BslFI GGGAC 1 cut(s) 259
BslI CCNNNNNNNGG 6 cut(s) 243, 247, 369, 524, 525, 526
BsmFI GGGAC 1 cut(s) 259
BsnI GGCC 6 cut(s) 264, 444, 530, 557, 924, 959
BsoBI CYCGRG 3 cut(s) 133, 242, 259
Bsp120I GGGCCC 2 cut(s) 262, 528
Bsp1286I GDGCHC 4 cut(s) 266, 435, 532, 717
Bsp143I GATC 5 cut(s) 124, 250, 480, 762, 1096
BspACI CCGC 5 cut(s) 238, 266, 294, 574, 955
BspANI GGCC 6 cut(s) 264, 444, 530, 557, 924, 959
BspDI ATCGAT 1 cut(s) 1095
BspLI GGNNCC 5 cut(s) 264, 417, 529, 530, 699
BspPI GGATC 3 cut(s) 258, 488, 770
BsrBI CCGCTC 1 cut(s) 268
BsrI ACTGG 2 cut(s) 352, 708
BssECI CCNNGG 8 cut(s) 241, 242, 259, 489, 490, 552, 771, 772
BssMI GATC 5 cut(s) 124, 250, 480, 762, 1096
BssNI GRCGYC 1 cut(s) 726
BssSI CACGAG 3 cut(s) 339, 446, 915
Bst2BI CACGAG 3 cut(s) 339, 446, 915
Bst2UI CCWGG 2 cut(s) 491, 773
Bst4CI ACNGT 4 cut(s) 278, 320, 600, 869
BstACI GRCGYC 1 cut(s) 726
BstC8I GCNNGC 4 cut(s) 266, 926, 957, 961
BstDEI CTNAG 3 cut(s) 523, 936, 1039
BstDSI CCRYGG 1 cut(s) 552
BstEII GGTNACC 2 cut(s) 358, 638
BstF5I GGATG 5 cut(s) 233, 466, 509, 535, 748
BstKTI GATC 5 cut(s) 127, 253, 483, 765, 1099
BstMBI GATC 5 cut(s) 124, 250, 480, 762, 1096
BstNI CCWGG 2 cut(s) 491, 773
BstNSI RCATGY 1 cut(s) 955
BstPI GGTNACC 2 cut(s) 358, 638
BstSCI CCNGG 6 cut(s) 241, 242, 258, 259, 489, 771
BstSFI CTRYAG 1 cut(s) 277
BstSLI GKGCMC 2 cut(s) 266, 532
Bsu15I ATCGAT 1 cut(s) 1095
Bsu36I CCTNAGG 1 cut(s) 523
BsuRI GGCC 6 cut(s) 264, 444, 530, 557, 924, 959
BsuTUI ATCGAT 1 cut(s) 1095
BtgI CCRYGG 1 cut(s) 552
BtgZI GCGATG 1 cut(s) 792
BtrI CACGTC 1 cut(s) 992
BtsCI GGATG 5 cut(s) 233, 466, 509, 535, 748
BtsIMutI CAGTG 3 cut(s) 274, 359, 715
Cac8I GCNNGC 4 cut(s) 266, 926, 957, 961
Cfr13I GGNCC 7 cut(s) 262, 263, 528, 529, 865, 922, 957
Cfr9I CCCGGG 2 cut(s) 242, 259
ClaI ATCGAT 1 cut(s) 1095
CseI GACGC 1 cut(s) 715
Csp6I GTAC 1 cut(s) 1063
CviAII CATG 4 cut(s) 283, 368, 952, 1070
CviQI GTAC 1 cut(s) 1063
DdeI CTNAG 3 cut(s) 523, 936, 1039
DpnI GATC 5 cut(s) 126, 252, 482, 764, 1098
DpnII GATC 5 cut(s) 124, 250, 480, 762, 1096
DraI TTTAAA 3 cut(s) 60, 207, 1173
DrdI GACNNNNNNGTC 1 cut(s) 540
DseDI GACNNNNNNGTC 1 cut(s) 540
EaeI YGGCCR 1 cut(s) 555
Ecl136II GAGCTC 2 cut(s) 433, 715
Eco147I AGGCCT 1 cut(s) 444
Eco24I GRGCYC 4 cut(s) 266, 435, 532, 717
Eco32I GATATC 1 cut(s) 1130
Eco47I GGWCC 1 cut(s) 865
Eco53kI GAGCTC 2 cut(s) 433, 715
Eco57I CTGAAG 4 cut(s) 210, 385, 441, 667
Eco81I CCTNAGG 1 cut(s) 523
Eco88I CYCGRG 3 cut(s) 133, 242, 259
Eco91I GGTNACC 2 cut(s) 358, 638
EcoICRI GAGCTC 2 cut(s) 433, 715
EcoO109I RGGNCCY 1 cut(s) 528
EcoO65I GGTNACC 2 cut(s) 358, 638
EcoRII CCWGG 2 cut(s) 489, 771
EcoRV GATATC 1 cut(s) 1130
EcoT38I GRGCYC 4 cut(s) 266, 435, 532, 717
FaeI CATG 4 cut(s) 286, 371, 955, 1073
FaqI GGGAC 1 cut(s) 259
FatI CATG 4 cut(s) 282, 367, 951, 1069
FauI CCCGC 3 cut(s) 245, 273, 948
FokI GGATG 5 cut(s) 220, 473, 516, 522, 755
FriOI GRGCYC 4 cut(s) 266, 435, 532, 717
FspBI CTAG 3 cut(s) 380, 660, 734
HaeIII GGCC 6 cut(s) 264, 444, 530, 557, 924, 959
HapII CCGG 2 cut(s) 243, 260
HgaI GACGC 1 cut(s) 715
Hin1I GRCGYC 1 cut(s) 726
Hin1II CATG 4 cut(s) 286, 371, 955, 1073
HpaII CCGG 2 cut(s) 243, 260
HphI GGTGA 3 cut(s) 352, 506, 632
Hpy166II GTNNAC 1 cut(s) 865
Hpy188I TCNGA 3 cut(s) 190, 404, 686
Hpy188III TCNNGA 3 cut(s) 37, 128, 339
Hpy8I GTNNAC 1 cut(s) 865
Hpy99I CGWCG 1 cut(s) 731
HpyAV CCTTC 2 cut(s) 397, 679
HpyCH4III ACNGT 4 cut(s) 278, 320, 600, 869
HpyCH4IV ACGT 2 cut(s) 564, 991
HpyCH4V TGCA 3 cut(s) 451, 1059, 1146
HpyF3I CTNAG 3 cut(s) 523, 936, 1039
HpySE526I ACGT 2 cut(s) 564, 991
Hsp92I GRCGYC 1 cut(s) 726
Hsp92II CATG 4 cut(s) 286, 371, 955, 1073
Kzo9I GATC 5 cut(s) 124, 250, 480, 762, 1096
LmnI GCTCC 3 cut(s) 273, 438, 720
MaeI CTAG 3 cut(s) 380, 660, 734
MaeII ACGT 2 cut(s) 564, 991
MaeIII GTNAC 5 cut(s) 229, 358, 506, 638, 888
MalI GATC 5 cut(s) 126, 252, 482, 764, 1098
MbiI CCGCTC 1 cut(s) 268
MboI GATC 5 cut(s) 124, 250, 480, 762, 1096
MboII GAAGA 1 cut(s) 1177
MhlI GDGCHC 4 cut(s) 266, 435, 532, 717
MmeI TCCRAC 3 cut(s) 139, 790, 851
MnlI CCTC 4 cut(s) 324, 455, 549, 604
MslI CAYNNNNRTG 1 cut(s) 917
MspI CCGG 2 cut(s) 243, 260
MspR9I CCNGG 6 cut(s) 243, 244, 260, 261, 491, 773
MvaI CCWGG 2 cut(s) 491, 773
NciI CCSGG 4 cut(s) 243, 244, 260, 261
NdeII GATC 5 cut(s) 124, 250, 480, 762, 1096
NlaIII CATG 4 cut(s) 286, 371, 955, 1073
NlaIV GGNNCC 5 cut(s) 264, 417, 529, 530, 699
NmuCI GTSAC 4 cut(s) 229, 358, 638, 888
NspI RCATGY 1 cut(s) 955
OliI CACNNNNGTG 1 cut(s) 917
PacI TTAATTAA 1 cut(s) 798
PasI CCCWGGG 2 cut(s) 490, 772
PceI AGGCCT 1 cut(s) 444
PshBI ATTAAT 1 cut(s) 798
PsiI TTATAA 1 cut(s) 1116
Psp124BI GAGCTC 2 cut(s) 435, 717
Psp6I CCWGG 2 cut(s) 489, 771
PspEI GGTNACC 2 cut(s) 358, 638
PspGI CCWGG 2 cut(s) 489, 771
PspN4I GGNNCC 5 cut(s) 264, 417, 529, 530, 699
PspOMI GGGCCC 2 cut(s) 262, 528
PspPI GGNCC 7 cut(s) 262, 263, 528, 529, 865, 922, 957
RsaI GTAC 1 cut(s) 1064
RsaNI GTAC 1 cut(s) 1063
RseI CAYNNNNRTG 1 cut(s) 917
SacI GAGCTC 2 cut(s) 435, 717
Sau3AI GATC 5 cut(s) 124, 250, 480, 762, 1096
Sau96I GGNCC 7 cut(s) 262, 263, 528, 529, 865, 922, 957
ScaI AGTACT 1 cut(s) 1064
ScrFI CCNGG 6 cut(s) 243, 244, 260, 261, 491, 773
SduI GDGCHC 4 cut(s) 266, 435, 532, 717
SfcI CTRYAG 1 cut(s) 277
SinI GGWCC 1 cut(s) 865
SmaI CCCGGG 2 cut(s) 244, 261
SmiMI CAYNNNNRTG 1 cut(s) 917
SseBI AGGCCT 1 cut(s) 444
SsiI CCGC 5 cut(s) 238, 266, 294, 574, 955
SspMI CTAG 3 cut(s) 380, 660, 734
SstI GAGCTC 2 cut(s) 435, 717
StuI AGGCCT 1 cut(s) 444
StyD4I CCNGG 6 cut(s) 241, 242, 258, 259, 489, 771
TaaI ACNGT 4 cut(s) 278, 320, 600, 869
TaiI ACGT 2 cut(s) 567, 994
TaqI TCGA 1 cut(s) 1095
TatI WGTACW 1 cut(s) 1062
TscAI CASTG 3 cut(s) 281, 359, 715
TseFI GTSAC 4 cut(s) 229, 358, 638, 888
Tsp45I GTSAC 4 cut(s) 229, 358, 638, 888
TspDTI ATGAA 1 cut(s) 165
TspGWI ACGGA 2 cut(s) 263, 710
TspMI CCCGGG 2 cut(s) 242, 259
TspRI CASTG 3 cut(s) 281, 359, 715
VpaK11BI GGWCC 1 cut(s) 865
VspI ATTAAT 1 cut(s) 798
XapI RAATTY 3 cut(s) 90, 152, 1168
XceI RCATGY 1 cut(s) 955
XmaI CCCGGG 2 cut(s) 242, 259
XspI CTAG 3 cut(s) 380, 660, 734
ZrmI AGTACT 1 cut(s) 1064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.