pycom808g00250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000808
OG0000158
Physical Location & Seq
Forward (+)
142288 .. 144667
2380 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom808g00250.16

Sequence Viewer

Length: 1674 bp
ATGTGTACTCAAGAGATCAAAAGCTTTTTAGGTGGCAAATGTACACAATTTTTCGATTTAGAGTGCATTTGGACTGTTTTCGGCATTACTAAAAACAAAGAATGTTCTACTAGGACCACAAGAATAAGTATTGCAAAGATCAAAATCTTAATGGGTGTAAAAACTATATACTTTTTATATTTTCACATGTGTGCAACTAGAACTCAAAAAATTAAAAGCTTATTGGGTGGCAAAAGTACACAGTTTTTCTATTTGAGTGCATTTGCATTGCTACCGGCATTAATAAAAACAAATGAGTGTGCATTCTACAACCTTTGGAACCTAAGCAATACTCAAAAGATTAAAAGCTTATTGGGTGTCAAAAGTAAACAGTTTTCTACTTGGAGTTCATTTAGACTGTTTTCAACATTACTAAAAACAAATGACAATGCGACAATAAGAATGAGTACTCAAAAGAACTATTCTAAAAATCCCCGCCTAGGCGCTAGGTGGACAATAAGAATAAGGTCCCGATTAATGCCTAAGCTTTTGAAAATTAAGAAAAGGTGCCAAGACCTACTGAGATCGACTTACTTAGACAAAAAATATATAAATTTAATTTTAATTTTGCATTTTGCATTTTATTTTTTTTTCAATAAATTCCTTGTTCCTCATGTTTTCATAATGTTCCAATACTTTATAATATATATATGTCACATCCTGGCCCGGGCCCCCACCACATCCCAGGGTCGACTCCACCGTAGCACGATATTGTCCGCTTTGGGCCCCGACCACGCCCTCACGGTTTTGTTTCTGGAAACTCACACGAGAACTTCCCAGTGGGTCACCCATCATAGGAGTTATCCTAGGTGCATTCCAGAATTTAGAAGTAAATCTTGGATCTCGATCAGAGATGATGCTGACTGGTACACCATGGTATTTAACGATCTTCAACACAAAGAGCTCAACGATCAACCACTACCCAAATGCCATCATAACCATTTCGTGTACGGCACGCTCCAATTTTTTATCAAGGTGTGTTATCTATATATAATATTTTATTTTATAGTTTATGATGAAATTATATATATTTTAAGTATAATTCACCTAGGGCCCCGCCTGGCATCTTTTGGAACCTTGCTCAAAAGATCAAAAGCTTATTCGTTGTCGGCACTAACAAAAACAAACGAACATGCATCTGATAACCATAGAAGCACTCAAAAGATCGAAAGCTTATTGGTTGTCAAAAGTACACAGTTTTATATTTGGAATGAATTTGGATTGTTTTGGGCATTACTAAAAACAAACGAGTGTAATGCACTACTAGAACCACAAGAACGAGTACTCAAAAGATCAAAAGCTTATTGGGTGTCAAAACTACACAGTTTTTTTATTCTGAGCGCATTTGGATTCCATTTTCAGCATAAACAAAAAAAAAAAAAAAGAACTAGCGTGTCTAAATGTCACATCCCGACCCAGGCCCCCACCACATCCCGAGCTCGACTCCACCGTAGCACGATATTGTCCGCTTTGGACCCCAACCACGCCCTCACGGTTTTGTTTTTGAGAACTCACACGAGAATTTCCCAATGGGTCACCCATCTTGTGATCCTTATATGTATATTCCCATCCCTACCTAGCACGAGGCCTTTTGCCGTAACACCTGTAGAATTTCCCAAAAAAGAACAGGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

558

Amino Acids

65.22

Weight (kDa)

10.03

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000158)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10163
malus_domestica MD04G1068000.v1.1 MD05G1130900.v1.1 MD06G1153600.v1.1 MD10G1255900.v1.1 MD10G1282000.v1.1 MD13G1075400.v1.1 MD13G1192400.v1.1
pyrus_communis pycom01g00130 pycom01g00390 pycom01g00700 pycom01g00720 pycom01g00750 pycom01g00850 pycom01g00870 pycom01g00910 pycom01g01250 pycom01g01560 pycom01g01680 pycom01g01860 pycom01g01900 pycom01g01910 pycom01g02200 pycom01g02260 pycom01g02270 pycom01g02280 pycom01g02330 pycom01g02480 pycom01g02720 pycom01g02730 pycom01g02750 pycom01g02770 pycom01g02780 pycom01g02860 pycom01g02910 pycom01g03540 pycom01g04090 pycom01g04100 pycom01g04150 pycom01g04250 pycom01g04430 pycom01g11810 pycom01g11860 pycom01g11940 pycom01g21380 pycom02g06650 pycom02g08490 pycom02g08600 pycom02g18230 pycom02g18450 pycom02g18800 pycom02g19150 pycom02g20740 pycom02g21340 pycom02g26210 pycom03g12060 pycom03g19060 pycom03g19100 pycom03g19120 pycom04g06890 pycom04g08960 pycom05g01790 pycom05g07910 pycom05g08080 pycom05g08100 pycom05g08570 pycom05g21500 pycom05g22400 pycom05g22510 pycom05g22620 pycom05g23020 pycom05g23720 pycom05g23890 pycom05g24040 pycom07g12080 pycom08g07930 pycom08g11940 pycom09g12120 pycom10g03890 pycom10g23360 pycom10g28250 pycom11g02850 pycom11g14370 pycom11g14400 pycom11g21840 pycom11g21860 pycom11g21980 pycom11g23580 pycom11g23650 pycom11g24050 pycom11g25610 pycom12424g00060 pycom1256g00130 pycom12661g00030 pycom12g09520 pycom12g09530 pycom12g09660 pycom12g09730 pycom12g11210 pycom12g16710 pycom12g16740 pycom12g16750 pycom13g08800 pycom13g18580 pycom13g18670 pycom13g18770 pycom13g22900 pycom13g23050 pycom13g23160 pycom13g23480 pycom13g23610 pycom13g23660 pycom13g23790 pycom13g23840 pycom13g24010 pycom13g24140 pycom13g24210 pycom13g24300 pycom13g24310 pycom13g24480 pycom13g24520 pycom13g24570 pycom13g24730 pycom13g24760 pycom13g24820 pycom13g25010 pycom13g25020 pycom13g25270 pycom13g25620 pycom13g25670 pycom13g25760 pycom13g25800 pycom13g25900 pycom13g26620 pycom13g26640 pycom13g26670 pycom13g26940 pycom13g27040 pycom13g27130 pycom13g27530 pycom13g27770 pycom13g27830 pycom13g27920 pycom13g27940 pycom13g28100 pycom13g28420 pycom13g28430 pycom13g28460 pycom13g28520 pycom13g29160 pycom14g05380 pycom15g03480 pycom15g04370 pycom15g27500 pycom16g13590 pycom16g13610 pycom16g13630 pycom16g14390 pycom16g18310 pycom17g01940 pycom17g04000 pycom17g16100 pycom17g16220 pycom17g16230 pycom17g16340 pycom17g16430 pycom17g16460 pycom17g16740 pycom17g16960 pycom17g17020 pycom17g17090 pycom17g17100 pycom17g17170 pycom17g17430 pycom17g17850 pycom17g18230 pycom17g18250 pycom17g18380 pycom17g21170 pycom17g21330 pycom520g00150 pycom520g00160 pycom808g00030 pycom808g00160 pycom808g00250 pycom808g00290
rosa_chinensis RchiOBHm_Chr5g0054451

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 680
AccB1I GGYRCC 1 cut(s) 546
AccI GTMKAC 1 cut(s) 730
AciI CCGC 4 cut(s) 475, 756, 1096, 1506
AclWI GGATC 2 cut(s) 887, 1582
AcsI RAATTY 6 cut(s) 592, 638, 860, 1253, 1560, 1649
AfaI GTAC 8 cut(s) 7, 43, 238, 448, 908, 989, 1231, 1323
AfiI CCNNNNNNNGG 4 cut(s) 479, 706, 834, 1456
AflIII ACRYGT 1 cut(s) 186
AgsI TTSAA 4 cut(s) 405, 532, 634, 932
AjnI CCWGG 4 cut(s) 699, 723, 1098, 1455
AjuI GAANNNNNNNTTGG 2 cut(s) 859, 891
AleI CACNNNNGTG 1 cut(s) 189
AluBI AGCT 9 cut(s) 24, 219, 348, 526, 943, 1136, 1212, 1340, 1478
AluI AGCT 9 cut(s) 24, 219, 348, 526, 943, 1136, 1212, 1340, 1478
Alw21I GWGCWC 2 cut(s) 945, 1480
AlwI GGATC 2 cut(s) 887, 1582
Ama87I CYCGRG 2 cut(s) 705, 1473
AoxI GGCC 6 cut(s) 702, 708, 763, 1091, 1458, 1625
ApaI GGGCCC 3 cut(s) 712, 767, 1095
ApoI RAATTY 6 cut(s) 592, 638, 860, 1253, 1560, 1649
AseI ATTAAT 2 cut(s) 281, 515
AspA2I CCTAGG 3 cut(s) 478, 845, 1087
AspLEI GCGC 2 cut(s) 485, 1382
AsuC2I CCSGG 2 cut(s) 706, 707
AsuHPI GGTGA 3 cut(s) 817, 1076, 1567
AvaI CYCGRG 2 cut(s) 705, 1473
AvaII GGWCC 3 cut(s) 114, 507, 1513
AvrII CCTAGG 3 cut(s) 478, 845, 1087
BaeGI GKGCMC 3 cut(s) 712, 767, 1095
BanI GGYRCC 1 cut(s) 546
BanII GRGCYC 5 cut(s) 712, 767, 945, 1095, 1480
BauI CACGAG 3 cut(s) 805, 1555, 1621
Bbv12I GWGCWC 2 cut(s) 945, 1480
BccI CCATC 4 cut(s) 837, 978, 1587, 1615
BceAI ACGGC 2 cut(s) 1006, 1619
BcgI CGANNNNNNTGC 2 cut(s) 1277, 1311
BciT130I CCWGG 4 cut(s) 701, 725, 1100, 1457
BcnI CCSGG 2 cut(s) 706, 707
BfaI CTAG 9 cut(s) 111, 198, 479, 486, 846, 1088, 1304, 1428, 1617
BfmI CTRYAG 1 cut(s) 1644
BfoI RGCGCY 1 cut(s) 486
BlnI CCTAGG 3 cut(s) 478, 845, 1087
BmcAI AGTACT 2 cut(s) 448, 1323
Bme1390I CCNGG 6 cut(s) 701, 706, 707, 725, 1100, 1457
Bme18I GGWCC 3 cut(s) 114, 507, 1513
BmeT110I CYCGRG 2 cut(s) 705, 1473
BmrFI CCNGG 6 cut(s) 701, 706, 707, 725, 1100, 1457
BmrI ACTGGG 1 cut(s) 811
BmsI GCATC 3 cut(s) 886, 1112, 1184
BmuI ACTGGG 1 cut(s) 811
BplI GAGNNNNNCTC 2 cut(s) 1467, 1499
Bpu10I CCTNAGC 2 cut(s) 323, 522
BpuMI CCSGG 2 cut(s) 706, 707
BsaBI GATNNNNATC 2 cut(s) 143, 884
BsaJI CCNNGG 8 cut(s) 478, 705, 723, 724, 845, 912, 1087, 1455
Bsc4I CCNNNNNNNGG 4 cut(s) 479, 706, 834, 1456
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 2 cut(s) 817, 908
Bse3DI GCAATG 1 cut(s) 266
Bse8I GATNNNNATC 2 cut(s) 143, 884
BseBI CCWGG 4 cut(s) 701, 725, 1100, 1457
BseDI CCNNGG 8 cut(s) 478, 705, 723, 724, 845, 912, 1087, 1455
BseGI GGATG 5 cut(s) 696, 719, 1446, 1469, 1607
BseJI GATNNNNATC 2 cut(s) 143, 884
BseLI CCNNNNNNNGG 4 cut(s) 479, 706, 834, 1456
BseMI GCAATG 1 cut(s) 266
BseMII CTCAG 2 cut(s) 551, 1367
BseNI ACTGG 2 cut(s) 817, 908
BseSI GKGCMC 3 cut(s) 712, 767, 1095
BshFI GGCC 6 cut(s) 704, 710, 765, 1093, 1460, 1627
BshNI GGYRCC 1 cut(s) 546
BsiHKAI GWGCWC 2 cut(s) 945, 1480
BsiHKCI CYCGRG 2 cut(s) 705, 1473
BsiSI CCGG 2 cut(s) 275, 706
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 4 cut(s) 479, 706, 834, 1456
BsmFI GGGAC 1 cut(s) 493
BsmI GAATGC 2 cut(s) 302, 852
BsnI GGCC 6 cut(s) 704, 710, 765, 1093, 1460, 1627
BsoBI CYCGRG 2 cut(s) 705, 1473
Bsp120I GGGCCC 3 cut(s) 708, 763, 1091
Bsp1286I GDGCHC 5 cut(s) 712, 767, 945, 1095, 1480
Bsp1407I TGTACA 1 cut(s) 41
Bsp19I CCATGG 1 cut(s) 912
BspACI CCGC 4 cut(s) 475, 756, 1096, 1506
BspANI GGCC 6 cut(s) 704, 710, 765, 1093, 1460, 1627
BspCNI CTCAG 2 cut(s) 552, 1368
BspPI GGATC 2 cut(s) 887, 1582
BspT107I GGYRCC 1 cut(s) 546
BsrDI GCAATG 1 cut(s) 266
BsrFI RCCGGY 1 cut(s) 274
BsrGI TGTACA 1 cut(s) 41
BsrI ACTGG 2 cut(s) 817, 908
BssAI RCCGGY 1 cut(s) 274
BssECI CCNNGG 8 cut(s) 478, 705, 723, 724, 845, 912, 1087, 1455
BssSI CACGAG 3 cut(s) 805, 1555, 1621
BssT1I CCWWGG 4 cut(s) 478, 845, 912, 1087
Bst2BI CACGAG 3 cut(s) 805, 1555, 1621
Bst2UI CCWGG 4 cut(s) 701, 725, 1100, 1457
BstAUI TGTACA 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 995
BstDEI CTNAG 5 cut(s) 323, 522, 560, 574, 1376
BstDSI CCRYGG 1 cut(s) 912
BstEII GGTNACC 2 cut(s) 823, 1573
BstF5I GGATG 5 cut(s) 696, 719, 1446, 1469, 1607
BstH2I RGCGCY 1 cut(s) 486
BstHHI GCGC 2 cut(s) 485, 1382
BstNI CCWGG 4 cut(s) 701, 725, 1100, 1457
BstNSI RCATGY 2 cut(s) 190, 1175
BstPI GGTNACC 2 cut(s) 823, 1573
BstSCI CCNGG 6 cut(s) 699, 704, 705, 723, 1098, 1455
BstSFI CTRYAG 1 cut(s) 1644
BstSLI GKGCMC 3 cut(s) 712, 767, 1095
BstX2I RGATCY 1 cut(s) 879
BstYI RGATCY 1 cut(s) 879
BsuRI GGCC 6 cut(s) 704, 710, 765, 1093, 1460, 1627
BtgI CCRYGG 1 cut(s) 912
BtsCI GGATG 5 cut(s) 696, 719, 1446, 1469, 1607
BtsIMutI CAGTG 1 cut(s) 824
Cac8I GCNNGC 1 cut(s) 995
CfoI GCGC 2 cut(s) 485, 1382
Cfr10I RCCGGY 1 cut(s) 274
Cfr9I CCCGGG 1 cut(s) 705
Csp6I GTAC 8 cut(s) 6, 42, 237, 447, 907, 988, 1230, 1322
CviAII CATG 4 cut(s) 187, 653, 913, 1172
CviQI GTAC 8 cut(s) 6, 42, 237, 447, 907, 988, 1230, 1322
DdeI CTNAG 5 cut(s) 323, 522, 560, 574, 1376
Ecl136II GAGCTC 2 cut(s) 943, 1478
Eco130I CCWWGG 4 cut(s) 478, 845, 912, 1087
Eco147I AGGCCT 1 cut(s) 1627
Eco24I GRGCYC 5 cut(s) 712, 767, 945, 1095, 1480
Eco47I GGWCC 3 cut(s) 114, 507, 1513
Eco53kI GAGCTC 2 cut(s) 943, 1478
Eco88I CYCGRG 2 cut(s) 705, 1473
Eco91I GGTNACC 2 cut(s) 823, 1573
EcoICRI GAGCTC 2 cut(s) 943, 1478
EcoO109I RGGNCCY 6 cut(s) 507, 709, 764, 1091, 1092, 1459
EcoO65I GGTNACC 2 cut(s) 823, 1573
EcoRII CCWGG 4 cut(s) 699, 723, 1098, 1455
EcoT14I CCWWGG 4 cut(s) 478, 845, 912, 1087
EcoT22I ATGCAT 1 cut(s) 1177
EcoT38I GRGCYC 5 cut(s) 712, 767, 945, 1095, 1480
ErhI CCWWGG 4 cut(s) 478, 845, 912, 1087
FaeI CATG 4 cut(s) 190, 656, 916, 1175
FalI AAGNNNNNCTT 2 cut(s) 859, 891
FaqI GGGAC 1 cut(s) 493
FatI CATG 4 cut(s) 186, 652, 912, 1171
FauI CCCGC 2 cut(s) 482, 1103
FblI GTMKAC 1 cut(s) 730
FokI GGATG 5 cut(s) 683, 706, 1433, 1456, 1594
FriOI GRGCYC 5 cut(s) 712, 767, 945, 1095, 1480
FspBI CTAG 9 cut(s) 111, 198, 479, 486, 846, 1088, 1304, 1428, 1617
GlaI GCGC 2 cut(s) 484, 1381
HaeII RGCGCY 1 cut(s) 486
HaeIII GGCC 6 cut(s) 704, 710, 765, 1093, 1460, 1627
HapII CCGG 2 cut(s) 275, 706
HhaI GCGC 2 cut(s) 485, 1382
Hin1II CATG 4 cut(s) 190, 656, 916, 1175
Hin6I GCGC 2 cut(s) 483, 1380
HinP1I GCGC 2 cut(s) 483, 1380
HincII GTYRAC 1 cut(s) 731
HindII GTYRAC 1 cut(s) 731
HindIII AAGCTT 7 cut(s) 22, 217, 346, 524, 1134, 1210, 1338
HinfI GANTC 3 cut(s) 732, 1389, 1482
HpaII CCGG 2 cut(s) 275, 706
HphI GGTGA 3 cut(s) 817, 1076, 1567
Hpy166II GTNNAC 9 cut(s) 6, 44, 239, 368, 492, 731, 909, 988, 1232
Hpy188I TCNGA 3 cut(s) 890, 1180, 1377
Hpy188III TCNNGA 7 cut(s) 11, 510, 794, 857, 883, 1450, 1473
Hpy8I GTNNAC 9 cut(s) 6, 44, 239, 368, 492, 731, 909, 988, 1232
HpyF3I CTNAG 5 cut(s) 323, 522, 560, 574, 1376
Hsp92II CATG 4 cut(s) 190, 656, 916, 1175
HspAI GCGC 2 cut(s) 483, 1380
LmnI GCTCC 1 cut(s) 1002
LweI GCATC 3 cut(s) 886, 1112, 1184
MaeI CTAG 9 cut(s) 111, 198, 479, 486, 846, 1088, 1304, 1428, 1617
MaeIII GTNAC 5 cut(s) 692, 823, 1442, 1573, 1636
MboII GAAGA 1 cut(s) 920
MflI RGATCY 1 cut(s) 879
MhlI GDGCHC 5 cut(s) 712, 767, 945, 1095, 1480
MlyI GAGTC 2 cut(s) 726, 1476
MnlI CCTC 4 cut(s) 660, 788, 1538, 1617
Mph1103I ATGCAT 1 cut(s) 1177
MslI CAYNNNNRTG 1 cut(s) 189
MspI CCGG 2 cut(s) 275, 706
MspR9I CCNGG 6 cut(s) 701, 706, 707, 725, 1100, 1457
Mva1269I GAATGC 2 cut(s) 302, 852
MvaI CCWGG 4 cut(s) 701, 725, 1100, 1457
NciI CCSGG 2 cut(s) 706, 707
NcoI CCATGG 1 cut(s) 912
NlaIII CATG 4 cut(s) 190, 656, 916, 1175
NmuCI GTSAC 4 cut(s) 692, 823, 1442, 1573
NsiI ATGCAT 1 cut(s) 1177
NspI RCATGY 2 cut(s) 190, 1175
OliI CACNNNNGTG 1 cut(s) 189
PasI CCCWGGG 1 cut(s) 724
PceI AGGCCT 1 cut(s) 1627
PciI ACATGT 1 cut(s) 186
PcsI WCGNNNNNNNCGW 2 cut(s) 736, 1486
PctI GAATGC 2 cut(s) 302, 852
PfeI GAWTC 1 cut(s) 1389
PleI GAGTC 2 cut(s) 726, 1476
PpsI GAGTC 2 cut(s) 726, 1476
PpuMI RGGWCCY 1 cut(s) 507
PscI ACATGT 1 cut(s) 186
PshBI ATTAAT 2 cut(s) 281, 515
PsiI TTATAA 1 cut(s) 680
Psp124BI GAGCTC 2 cut(s) 945, 1480
Psp5II RGGWCCY 1 cut(s) 507
Psp6I CCWGG 4 cut(s) 699, 723, 1098, 1455
PspEI GGTNACC 2 cut(s) 823, 1573
PspGI CCWGG 4 cut(s) 699, 723, 1098, 1455
PspOMI GGGCCC 3 cut(s) 708, 763, 1091
PspPPI RGGWCCY 1 cut(s) 507
PsuI RGATCY 1 cut(s) 879
RsaI GTAC 8 cut(s) 7, 43, 238, 448, 908, 989, 1231, 1323
RsaNI GTAC 8 cut(s) 6, 42, 237, 447, 907, 988, 1230, 1322
RseI CAYNNNNRTG 1 cut(s) 189
SacI GAGCTC 2 cut(s) 945, 1480
SalI GTCGAC 1 cut(s) 729
ScaI AGTACT 2 cut(s) 448, 1323
SchI GAGTC 2 cut(s) 726, 1476
ScrFI CCNGG 6 cut(s) 701, 706, 707, 725, 1100, 1457
SduI GDGCHC 5 cut(s) 712, 767, 945, 1095, 1480
SfaNI GCATC 3 cut(s) 886, 1112, 1184
SfcI CTRYAG 1 cut(s) 1644
SinI GGWCC 3 cut(s) 114, 507, 1513
SmaI CCCGGG 1 cut(s) 707
SmiMI CAYNNNNRTG 1 cut(s) 189
SmlI CTYRAG 1 cut(s) 9
SmoI CTYRAG 1 cut(s) 9
SrfI GCCCGGGC 1 cut(s) 707
SseBI AGGCCT 1 cut(s) 1627
SsiI CCGC 4 cut(s) 475, 756, 1096, 1506
SspI AATATT 1 cut(s) 1035
SspMI CTAG 9 cut(s) 111, 198, 479, 486, 846, 1088, 1304, 1428, 1617
SstI GAGCTC 2 cut(s) 945, 1480
StuI AGGCCT 1 cut(s) 1627
StyD4I CCNGG 6 cut(s) 699, 704, 705, 723, 1098, 1455
StyI CCWWGG 4 cut(s) 478, 845, 912, 1087
TaqI TCGA 6 cut(s) 54, 566, 730, 884, 1206, 1480
TatI WGTACW 6 cut(s) 5, 41, 236, 446, 1229, 1321
TfiI GAWTC 1 cut(s) 1389
TscAI CASTG 1 cut(s) 824
TseFI GTSAC 4 cut(s) 692, 823, 1442, 1573
Tsp45I GTSAC 4 cut(s) 692, 823, 1442, 1573
TspDTI ATGAA 4 cut(s) 378, 649, 1071, 1266
TspMI CCCGGG 1 cut(s) 705
TspRI CASTG 1 cut(s) 824
VpaK11BI GGWCC 3 cut(s) 114, 507, 1513
VspI ATTAAT 2 cut(s) 281, 515
XapI RAATTY 6 cut(s) 592, 638, 860, 1253, 1560, 1649
XceI RCATGY 2 cut(s) 190, 1175
XmaI CCCGGG 1 cut(s) 705
XmaJI CCTAGG 3 cut(s) 478, 845, 1087
XmiI GTMKAC 1 cut(s) 730
XspI CTAG 9 cut(s) 111, 198, 479, 486, 846, 1088, 1304, 1428, 1617
ZrmI AGTACT 2 cut(s) 448, 1323
Zsp2I ATGCAT 1 cut(s) 1177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.