pycom13g23660

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000158
Physical Location & Seq
Forward (+)
21103235 .. 21105153
1919 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g23660.8

Sequence Viewer

Length: 1341 bp
ATGGCTTATTACTTTAAATACTCCATGTTACAACTAAACAAAATATCAATTGAAAAAAAAAAAAAAACAAAAAAAAGGAGAATTCACAGATCCCTGAGGTTTCACATATCCCTCCATTTACAAAGCCCAGTCGTCTTCGTCGTGTATCTCAACAAAATACAATTTGAACGAACCAGATCAAAGAAACCAATCCAATTCCACAAATTGCTTCAACAATTCAAGAATTCAAATTTTCGGACGAAGACGATGGAAAATCCCAATCGGCCCTTATTTTTACTAGGCAAAAAGAAAAAAAGAAAAAGAAAAACTGAGGTGGGGAAAGGATTGTTGACACCCTATTCTTCGCTGACTCTTCATTTGTTTTTTAGCTTAGTTAAAATGGTTTCTGAGATTTTGTCCATCATTCATTATTTTGGTCATTCCGTTAAAAACTCCGTTAAGTGTTGCGGAGTTTTTGGCCGAAAGTTTAGGCAATTTTTAAAGTTTCGTAACTCAATCGAAAGTGTAGAACAAGATTATATCTATTTGGAAGCCCAATGGTTGCCGGAGATGGCCGGAAAATATCCTAAAGTGTCCATAACTTCTTCAATATTCAACAAAATTGAAGAATGGTACCTTTCTCGATGGCTATATCACCGTGGTTTGGCCGGAAAACGTTTCGAAATTGGCTGTCTTAGTCTCGAGTTAGAATTAGCCGTCGAGGAAGGTACCATTCTCTTCATCTCGTTGAGAAGTATGATTTTTGTTTTCGAATCACTCGATTTCATTGAGCGAATTTTCCAGTTTTCCCACAGACTAGGCACATTTCAGGCTATTTTCCAGCCATCTCCAGTAACCATTGGGCTTCCAAATAGGTATAATCTTGTTCTACACTTTCCTATCTTCATTTTGATATATTATGGGTCGAATTTGGTTAAGAAACGATTGAGTTACAAAACTTTGAAAATTGCCCAAACTTTCTGTCACATCCCGGCCCGGGACGGATCACTTCCCAGCTTACCATTCCCTCACGGTTTTGTTTTTTGGGAACTCACGAGCAACTTCTCAGTGGGTCACCCATCATGGGATTGCTCTACTAGTGAGCTCCCAAAAGGTCTCGTGCTAGATAAGGATGGAAATATACATATAAGGATCACTCCCCTAAGCGATGTGGGATGTCACACTTTCGGCCAAGAGCTCTCATTTTGGCTAAAAAGCCTTTGTTTTTTAATTGGTTCCGATCCTACAATTTGTTTAGGTACAACATGGCTTATTATATATAATAAATCCTCGCATGTTCTCAAAATCGTCGGTAAAAACAATGTAGCAGTACCCCTAGCAAACAAAGCGTCCCTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

447

Amino Acids

51.78

Weight (kDa)

9.96

Isoelectric Point (pI)

51.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000158)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10163
malus_domestica MD04G1068000.v1.1 MD05G1130900.v1.1 MD06G1153600.v1.1 MD10G1255900.v1.1 MD10G1282000.v1.1 MD13G1075400.v1.1 MD13G1192400.v1.1
pyrus_communis pycom01g00130 pycom01g00390 pycom01g00700 pycom01g00720 pycom01g00750 pycom01g00850 pycom01g00870 pycom01g00910 pycom01g01250 pycom01g01560 pycom01g01680 pycom01g01860 pycom01g01900 pycom01g01910 pycom01g02200 pycom01g02260 pycom01g02270 pycom01g02280 pycom01g02330 pycom01g02480 pycom01g02720 pycom01g02730 pycom01g02750 pycom01g02770 pycom01g02780 pycom01g02860 pycom01g02910 pycom01g03540 pycom01g04090 pycom01g04100 pycom01g04150 pycom01g04250 pycom01g04430 pycom01g11810 pycom01g11860 pycom01g11940 pycom01g21380 pycom02g06650 pycom02g08490 pycom02g08600 pycom02g18230 pycom02g18450 pycom02g18800 pycom02g19150 pycom02g20740 pycom02g21340 pycom02g26210 pycom03g12060 pycom03g19060 pycom03g19100 pycom03g19120 pycom04g06890 pycom04g08960 pycom05g01790 pycom05g07910 pycom05g08080 pycom05g08100 pycom05g08570 pycom05g21500 pycom05g22400 pycom05g22510 pycom05g22620 pycom05g23020 pycom05g23720 pycom05g23890 pycom05g24040 pycom07g12080 pycom08g07930 pycom08g11940 pycom09g12120 pycom10g03890 pycom10g23360 pycom10g28250 pycom11g02850 pycom11g14370 pycom11g14400 pycom11g21840 pycom11g21860 pycom11g21980 pycom11g23580 pycom11g23650 pycom11g24050 pycom11g25610 pycom12424g00060 pycom1256g00130 pycom12661g00030 pycom12g09520 pycom12g09530 pycom12g09660 pycom12g09730 pycom12g11210 pycom12g16710 pycom12g16740 pycom12g16750 pycom13g08800 pycom13g18580 pycom13g18670 pycom13g18770 pycom13g22900 pycom13g23050 pycom13g23160 pycom13g23480 pycom13g23610 pycom13g23660 pycom13g23790 pycom13g23840 pycom13g24010 pycom13g24140 pycom13g24210 pycom13g24300 pycom13g24310 pycom13g24480 pycom13g24520 pycom13g24570 pycom13g24730 pycom13g24760 pycom13g24820 pycom13g25010 pycom13g25020 pycom13g25270 pycom13g25620 pycom13g25670 pycom13g25760 pycom13g25800 pycom13g25900 pycom13g26620 pycom13g26640 pycom13g26670 pycom13g26940 pycom13g27040 pycom13g27130 pycom13g27530 pycom13g27770 pycom13g27830 pycom13g27920 pycom13g27940 pycom13g28100 pycom13g28420 pycom13g28430 pycom13g28460 pycom13g28520 pycom13g29160 pycom14g05380 pycom15g03480 pycom15g04370 pycom15g27500 pycom16g13590 pycom16g13610 pycom16g13630 pycom16g14390 pycom16g18310 pycom17g01940 pycom17g04000 pycom17g16100 pycom17g16220 pycom17g16230 pycom17g16340 pycom17g16430 pycom17g16460 pycom17g16740 pycom17g16960 pycom17g17020 pycom17g17090 pycom17g17100 pycom17g17170 pycom17g17430 pycom17g17850 pycom17g18230 pycom17g18250 pycom17g18380 pycom17g21170 pycom17g21330 pycom520g00150 pycom520g00160 pycom808g00030 pycom808g00160 pycom808g00250 pycom808g00290
rosa_chinensis RchiOBHm_Chr5g0054451

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 612, 707
AccB1I GGYRCC 2 cut(s) 612, 707
AciI CCGC 1 cut(s) 447
AclI AACGTT 1 cut(s) 655
AclWI GGATC 4 cut(s) 84, 991, 1139, 1214
AcoI YGGCCR 4 cut(s) 457, 552, 645, 1168
AcsI RAATTY 5 cut(s) 81, 223, 229, 774, 907
AfaI GTAC 4 cut(s) 614, 709, 1240, 1311
AfiI CCNNNNNNNGG 3 cut(s) 643, 976, 1063
AgsI TTSAA 9 cut(s) 53, 167, 212, 220, 228, 588, 595, 605, 943
AhlI ACTAGT 1 cut(s) 1076
AluBI AGCT 4 cut(s) 369, 996, 1084, 1177
AluI AGCT 4 cut(s) 369, 996, 1084, 1177
Alw21I GWGCWC 2 cut(s) 1086, 1179
Alw26I GTCTC 2 cut(s) 683, 1100
AlwI GGATC 4 cut(s) 84, 991, 1139, 1214
Ama87I CYCGRG 2 cut(s) 680, 975
AoxI GGCC 6 cut(s) 263, 457, 552, 645, 972, 1168
ApoI RAATTY 5 cut(s) 81, 223, 229, 774, 907
ArsI GACNNNNNNTTYG 5 cut(s) 340, 372, 946, 978, 1313
Asp718I GGTACC 2 cut(s) 612, 707
AspS9I GGNCC 2 cut(s) 264, 973
AsuC2I CCSGG 3 cut(s) 971, 976, 977
AsuHPI GGTGA 2 cut(s) 626, 1046
AsuII TTCGAA 2 cut(s) 660, 750
AvaI CYCGRG 2 cut(s) 680, 975
AxyI CCTNAGG 1 cut(s) 95
BanI GGYRCC 2 cut(s) 612, 707
BanII GRGCYC 2 cut(s) 1086, 1179
BauI CACGAG 2 cut(s) 1033, 1097
BbsI GAAGAC 2 cut(s) 127, 248
Bbv12I GWGCWC 2 cut(s) 1086, 1179
BccI CCATC 7 cut(s) 241, 407, 544, 618, 832, 1066, 1106
BceAI ACGGC 1 cut(s) 680
BcnI CCSGG 3 cut(s) 971, 976, 977
BcoDI GTCTC 2 cut(s) 683, 1100
BcuI ACTAGT 1 cut(s) 1076
BfaI CTAG 5 cut(s) 278, 797, 1077, 1103, 1316
Bme1390I CCNGG 3 cut(s) 971, 976, 977
BmeT110I CYCGRG 2 cut(s) 680, 975
BmgT120I GGNCC 2 cut(s) 264, 973
BmiI GGNNCC 3 cut(s) 614, 709, 1216
BmrFI CCNGG 3 cut(s) 971, 976, 977
BmrI ACTGGG 1 cut(s) 122
BmuI ACTGGG 1 cut(s) 122
BpiI GAAGAC 2 cut(s) 127, 248
BpmI CTGGAG 1 cut(s) 813
Bpu10I CCTNAGC 1 cut(s) 1142
Bpu14I TTCGAA 2 cut(s) 660, 750
BpuMI CCSGG 3 cut(s) 971, 976, 977
BsaI GGTCTC 1 cut(s) 1100
BsaJI CCNNGG 2 cut(s) 637, 975
Bsc4I CCNNNNNNNGG 3 cut(s) 643, 976, 1063
Bse1I ACTGG 3 cut(s) 128, 781, 830
Bse21I CCTNAGG 1 cut(s) 95
BseDI CCNNGG 2 cut(s) 637, 975
BseGI GGATG 3 cut(s) 966, 1117, 1160
BseLI CCNNNNNNNGG 3 cut(s) 643, 976, 1063
BseMII CTCAG 4 cut(s) 86, 300, 378, 1059
BseNI ACTGG 3 cut(s) 128, 781, 830
BseYI CCCAGC 1 cut(s) 992
BshFI GGCC 6 cut(s) 265, 459, 554, 647, 974, 1170
BshNI GGYRCC 2 cut(s) 612, 707
BsiHKAI GWGCWC 2 cut(s) 1086, 1179
BsiHKCI CYCGRG 2 cut(s) 680, 975
BsiSI CCGG 5 cut(s) 545, 555, 648, 971, 976
BslFI GGGAC 2 cut(s) 992, 1315
BslI CCNNNNNNNGG 3 cut(s) 643, 976, 1063
BsmAI GTCTC 2 cut(s) 683, 1100
BsmFI GGGAC 2 cut(s) 992, 1315
BsnI GGCC 6 cut(s) 265, 459, 554, 647, 974, 1170
Bso31I GGTCTC 1 cut(s) 1100
BsoBI CYCGRG 2 cut(s) 680, 975
Bsp119I TTCGAA 2 cut(s) 660, 750
Bsp1286I GDGCHC 2 cut(s) 1086, 1179
Bsp143I GATC 5 cut(s) 89, 176, 983, 1131, 1219
BspACI CCGC 1 cut(s) 447
BspANI GGCC 6 cut(s) 265, 459, 554, 647, 974, 1170
BspCNI CTCAG 4 cut(s) 87, 301, 379, 1058
BspLI GGNNCC 3 cut(s) 614, 709, 1216
BspPI GGATC 4 cut(s) 84, 991, 1139, 1214
BspT104I TTCGAA 2 cut(s) 660, 750
BspT107I GGYRCC 2 cut(s) 612, 707
BspTNI GGTCTC 1 cut(s) 1100
BsrI ACTGG 3 cut(s) 128, 781, 830
BssECI CCNNGG 2 cut(s) 637, 975
BssMI GATC 5 cut(s) 89, 176, 983, 1131, 1219
BssSI CACGAG 2 cut(s) 1033, 1097
Bst2BI CACGAG 2 cut(s) 1033, 1097
Bst4CI ACNGT 2 cut(s) 638, 1013
Bst6I CTCTTC 2 cut(s) 357, 722
BstBI TTCGAA 2 cut(s) 660, 750
BstDEI CTNAG 7 cut(s) 95, 309, 370, 387, 674, 1045, 1142
BstDSI CCRYGG 1 cut(s) 637
BstEII GGTNACC 1 cut(s) 1052
BstF5I GGATG 3 cut(s) 966, 1117, 1160
BstKTI GATC 5 cut(s) 92, 179, 986, 1134, 1222
BstMAI GTCTC 2 cut(s) 683, 1100
BstMBI GATC 5 cut(s) 89, 176, 983, 1131, 1219
BstMWI GCNNNNNNNGC 1 cut(s) 1325
BstNSI RCATGY 1 cut(s) 1277
BstPI GGTNACC 1 cut(s) 1052
BstSCI CCNGG 3 cut(s) 969, 974, 975
BstV2I GAAGAC 2 cut(s) 127, 248
BstX2I RGATCY 1 cut(s) 89
BstYI RGATCY 1 cut(s) 89
Bsu36I CCTNAGG 1 cut(s) 95
BsuRI GGCC 6 cut(s) 265, 459, 554, 647, 974, 1170
BtgI CCRYGG 1 cut(s) 637
BtgZI GCGATG 1 cut(s) 1161
BtsCI GGATG 3 cut(s) 966, 1117, 1160
BtsIMutI CAGTG 1 cut(s) 1053
Cfr13I GGNCC 2 cut(s) 264, 973
Cfr9I CCCGGG 1 cut(s) 975
CseI GACGC 1 cut(s) 1317
Csp6I GTAC 4 cut(s) 613, 708, 1239, 1310
CviAII CATG 4 cut(s) 25, 1062, 1245, 1274
CviQI GTAC 4 cut(s) 613, 708, 1239, 1310
DdeI CTNAG 7 cut(s) 95, 309, 370, 387, 674, 1045, 1142
DpnI GATC 5 cut(s) 91, 178, 985, 1133, 1221
DpnII GATC 5 cut(s) 89, 176, 983, 1131, 1219
DraI TTTAAA 2 cut(s) 16, 480
EaeI YGGCCR 4 cut(s) 457, 552, 645, 1168
Eam1104I CTCTTC 2 cut(s) 357, 722
EarI CTCTTC 2 cut(s) 357, 722
Ecl136II GAGCTC 2 cut(s) 1084, 1177
Eco24I GRGCYC 2 cut(s) 1086, 1179
Eco31I GGTCTC 1 cut(s) 1100
Eco53kI GAGCTC 2 cut(s) 1084, 1177
Eco81I CCTNAGG 1 cut(s) 95
Eco88I CYCGRG 2 cut(s) 680, 975
Eco91I GGTNACC 1 cut(s) 1052
EcoICRI GAGCTC 2 cut(s) 1084, 1177
EcoO65I GGTNACC 1 cut(s) 1052
EcoRI GAATTC 2 cut(s) 81, 223
EcoT38I GRGCYC 2 cut(s) 1086, 1179
FaeI CATG 4 cut(s) 28, 1065, 1248, 1277
FalI AAGNNNNNCTT 1 cut(s) 1317
FaqI GGGAC 2 cut(s) 992, 1315
FatI CATG 4 cut(s) 24, 1061, 1244, 1273
FokI GGATG 3 cut(s) 953, 1124, 1167
FriOI GRGCYC 2 cut(s) 1086, 1179
FspBI CTAG 5 cut(s) 278, 797, 1077, 1103, 1316
GsaI CCCAGC 1 cut(s) 996
GsuI CTGGAG 1 cut(s) 813
HaeIII GGCC 6 cut(s) 265, 459, 554, 647, 974, 1170
HapII CCGG 5 cut(s) 545, 555, 648, 971, 976
HgaI GACGC 1 cut(s) 1317
Hin1II CATG 4 cut(s) 28, 1065, 1248, 1277
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 2 cut(s) 349, 752
HpaII CCGG 5 cut(s) 545, 555, 648, 971, 976
HphI GGTGA 2 cut(s) 626, 1046
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 3 cut(s) 237, 388, 1219
Hpy188III TCNNGA 4 cut(s) 220, 621, 680, 1033
Hpy8I GTNNAC 1 cut(s) 330
Hpy99I CGWCG 3 cut(s) 143, 701, 1292
HpyAV CCTTC 1 cut(s) 698
HpyCH4III ACNGT 2 cut(s) 638, 1013
HpyCH4IV ACGT 1 cut(s) 655
HpyF10VI GCNNNNNNNGC 1 cut(s) 1325
HpyF3I CTNAG 7 cut(s) 95, 309, 370, 387, 674, 1045, 1142
HpySE526I ACGT 1 cut(s) 655
Hsp92II CATG 4 cut(s) 28, 1065, 1248, 1277
KpnI GGTACC 2 cut(s) 616, 711
Kzo9I GATC 5 cut(s) 89, 176, 983, 1131, 1219
LmnI GCTCC 1 cut(s) 1089
MaeI CTAG 5 cut(s) 278, 797, 1077, 1103, 1316
MaeII ACGT 1 cut(s) 655
MaeIII GTNAC 7 cut(s) 27, 488, 832, 929, 962, 1052, 1157
MalI GATC 5 cut(s) 91, 178, 985, 1133, 1221
MboI GATC 5 cut(s) 89, 176, 983, 1131, 1219
MboII GAAGA 8 cut(s) 127, 253, 333, 344, 576, 617, 709, 874
MfeI CAATTG 1 cut(s) 48
MflI RGATCY 1 cut(s) 89
MhlI GDGCHC 2 cut(s) 1086, 1179
MlyI GAGTC 1 cut(s) 343
MnlI CCTC 6 cut(s) 90, 122, 304, 694, 1017, 1279
MseI TTAA 8 cut(s) 15, 375, 426, 438, 479, 915, 1208, 1335
MspI CCGG 5 cut(s) 545, 555, 648, 971, 976
MspR9I CCNGG 3 cut(s) 971, 976, 977
MunI CAATTG 1 cut(s) 48
MwoI GCNNNNNNNGC 1 cut(s) 1325
NciI CCSGG 3 cut(s) 971, 976, 977
NdeII GATC 5 cut(s) 89, 176, 983, 1131, 1219
NlaIII CATG 4 cut(s) 28, 1065, 1248, 1277
NlaIV GGNNCC 3 cut(s) 614, 709, 1216
NmuCI GTSAC 3 cut(s) 962, 1052, 1157
NspI RCATGY 1 cut(s) 1277
NspV TTCGAA 2 cut(s) 660, 750
PaeR7I CTCGAG 1 cut(s) 680
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 756
PfeI GAWTC 1 cut(s) 752
PleI GAGTC 1 cut(s) 343
PpsI GAGTC 1 cut(s) 343
Psp124BI GAGCTC 2 cut(s) 1086, 1179
Psp1406I AACGTT 1 cut(s) 655
PspEI GGTNACC 1 cut(s) 1052
PspFI CCCAGC 1 cut(s) 992
PspN4I GGNNCC 3 cut(s) 614, 709, 1216
PspPI GGNCC 2 cut(s) 264, 973
PsuI RGATCY 1 cut(s) 89
RsaI GTAC 4 cut(s) 614, 709, 1240, 1311
RsaNI GTAC 4 cut(s) 613, 708, 1239, 1310
SacI GAGCTC 2 cut(s) 1086, 1179
SaqAI TTAA 8 cut(s) 15, 375, 426, 438, 479, 915, 1208, 1335
Sau3AI GATC 5 cut(s) 89, 176, 983, 1131, 1219
Sau96I GGNCC 2 cut(s) 264, 973
SchI GAGTC 1 cut(s) 343
ScrFI CCNGG 3 cut(s) 971, 976, 977
SduI GDGCHC 2 cut(s) 1086, 1179
Sfr274I CTCGAG 1 cut(s) 680
SfuI TTCGAA 2 cut(s) 660, 750
SlaI CTCGAG 1 cut(s) 680
SmaI CCCGGG 1 cut(s) 977
SmlI CTYRAG 1 cut(s) 680
SmoI CTYRAG 1 cut(s) 680
SpeI ACTAGT 1 cut(s) 1076
SsiI CCGC 1 cut(s) 447
SspI AATATT 1 cut(s) 591
SspMI CTAG 5 cut(s) 278, 797, 1077, 1103, 1316
SstI GAGCTC 2 cut(s) 1086, 1179
StyD4I CCNGG 3 cut(s) 969, 974, 975
TaaI ACNGT 2 cut(s) 638, 1013
TaiI ACGT 1 cut(s) 658
TaqI TCGA 8 cut(s) 498, 622, 660, 681, 699, 750, 759, 905
TfiI GAWTC 1 cut(s) 752
Tru1I TTAA 8 cut(s) 15, 375, 426, 438, 479, 915, 1208, 1335
Tru9I TTAA 8 cut(s) 15, 375, 426, 438, 479, 915, 1208, 1335
TscAI CASTG 1 cut(s) 1053
TseFI GTSAC 3 cut(s) 962, 1052, 1157
Tsp45I GTSAC 3 cut(s) 962, 1052, 1157
TspDTI ATGAA 5 cut(s) 344, 395, 709, 754, 874
TspGWI ACGGA 3 cut(s) 412, 424, 996
TspMI CCCGGG 1 cut(s) 975
TspRI CASTG 1 cut(s) 1053
XapI RAATTY 5 cut(s) 81, 223, 229, 774, 907
XceI RCATGY 1 cut(s) 1277
XhoI CTCGAG 1 cut(s) 680
XmaI CCCGGG 1 cut(s) 975
XspI CTAG 5 cut(s) 278, 797, 1077, 1103, 1316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.