pycom13g25020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000158
Physical Location & Seq
Forward (+)
21973427 .. 21974426
1000 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g25020.2

Sequence Viewer

Length: 843 bp
ATGGGTGACCCACTGGGAAGTTTTCATGTGAGTTCCCAGAAACAAAACCGTGAGGGCGTGGTCGGGGCCCAAAGCAGACAATATCATCCGGGACACATCCCTCACGGTTTTATTTTTGGTAACTCCCACGAGAACTTCCTAGTGGGTCACCCATCCTGGGATTGCTCTCGTGCAAACTCGCTTAACTTTGGAGTTCCTACGGAACTCAAAGCCAGTGAGCTCGCAAAAGGCCATATCCTGACCTGGGCGCCTACCATATCCCGGGCTCGACTCCACCGTAATACGATATTATTTGAGAACTTTCTAGTAGGTCACCCATCCTGGGATTGCTCTCGCGCGAACTCACTTAACTTCGGAGTTCCGATGGAACCCAAAGCTAGTGAGCTCCCAAAAGGCCTCGTGCTAGGTAGAGATCGGAATATACATATAAGGCTTATAGGATCCACTCCCCTAGGCGATGTGGGATGTTACAATCCACCCACCTTAGGGGCACGACGTCTTCATCGGCACACTTCTGGCCAGGGATTGCTTCTGATACCAAATTGTCACATCTCGGCCCGGGCACCCACCACATCCCAGGCTCAACTCCACCGTAGCATGATATTTTCCGTTTCTGGCCCCGACCACACCCTCACGGTTTTGTTTCTGGGAACTTGCATGAGAACTTCTAGTGGGTCACCCATTGTGGGAATGCTCTCGTCCGAACTCAGATGGGAATATACATATAAGGCTTACATGATCCACTCCCCTAGGCGATGTGGGATGTTACAAGAGACTATGGTTTTGTCTTCTTTAACCCCATTTTCTTTGGTGATGACTTCTAACTTCTTTTCAGACTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

281

Amino Acids

30.87

Weight (kDa)

9.47

Isoelectric Point (pI)

48.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000158)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10163
malus_domestica MD04G1068000.v1.1 MD05G1130900.v1.1 MD06G1153600.v1.1 MD10G1255900.v1.1 MD10G1282000.v1.1 MD13G1075400.v1.1 MD13G1192400.v1.1
pyrus_communis pycom01g00130 pycom01g00390 pycom01g00700 pycom01g00720 pycom01g00750 pycom01g00850 pycom01g00870 pycom01g00910 pycom01g01250 pycom01g01560 pycom01g01680 pycom01g01860 pycom01g01900 pycom01g01910 pycom01g02200 pycom01g02260 pycom01g02270 pycom01g02280 pycom01g02330 pycom01g02480 pycom01g02720 pycom01g02730 pycom01g02750 pycom01g02770 pycom01g02780 pycom01g02860 pycom01g02910 pycom01g03540 pycom01g04090 pycom01g04100 pycom01g04150 pycom01g04250 pycom01g04430 pycom01g11810 pycom01g11860 pycom01g11940 pycom01g21380 pycom02g06650 pycom02g08490 pycom02g08600 pycom02g18230 pycom02g18450 pycom02g18800 pycom02g19150 pycom02g20740 pycom02g21340 pycom02g26210 pycom03g12060 pycom03g19060 pycom03g19100 pycom03g19120 pycom04g06890 pycom04g08960 pycom05g01790 pycom05g07910 pycom05g08080 pycom05g08100 pycom05g08570 pycom05g21500 pycom05g22400 pycom05g22510 pycom05g22620 pycom05g23020 pycom05g23720 pycom05g23890 pycom05g24040 pycom07g12080 pycom08g07930 pycom08g11940 pycom09g12120 pycom10g03890 pycom10g23360 pycom10g28250 pycom11g02850 pycom11g14370 pycom11g14400 pycom11g21840 pycom11g21860 pycom11g21980 pycom11g23580 pycom11g23650 pycom11g24050 pycom11g25610 pycom12424g00060 pycom1256g00130 pycom12661g00030 pycom12g09520 pycom12g09530 pycom12g09660 pycom12g09730 pycom12g11210 pycom12g16710 pycom12g16740 pycom12g16750 pycom13g08800 pycom13g18580 pycom13g18670 pycom13g18770 pycom13g22900 pycom13g23050 pycom13g23160 pycom13g23480 pycom13g23610 pycom13g23660 pycom13g23790 pycom13g23840 pycom13g24010 pycom13g24140 pycom13g24210 pycom13g24300 pycom13g24310 pycom13g24480 pycom13g24520 pycom13g24570 pycom13g24730 pycom13g24760 pycom13g24820 pycom13g25010 pycom13g25020 pycom13g25270 pycom13g25620 pycom13g25670 pycom13g25760 pycom13g25800 pycom13g25900 pycom13g26620 pycom13g26640 pycom13g26670 pycom13g26940 pycom13g27040 pycom13g27130 pycom13g27530 pycom13g27770 pycom13g27830 pycom13g27920 pycom13g27940 pycom13g28100 pycom13g28420 pycom13g28430 pycom13g28460 pycom13g28520 pycom13g29160 pycom14g05380 pycom15g03480 pycom15g04370 pycom15g27500 pycom16g13590 pycom16g13610 pycom16g13630 pycom16g14390 pycom16g18310 pycom17g01940 pycom17g04000 pycom17g16100 pycom17g16220 pycom17g16230 pycom17g16340 pycom17g16430 pycom17g16460 pycom17g16740 pycom17g16960 pycom17g17020 pycom17g17090 pycom17g17100 pycom17g17170 pycom17g17430 pycom17g17850 pycom17g18230 pycom17g18250 pycom17g18380 pycom17g21170 pycom17g21330 pycom520g00150 pycom520g00160 pycom808g00030 pycom808g00160 pycom808g00250 pycom808g00290
rosa_chinensis RchiOBHm_Chr5g0054451

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 499
AccB1I GGYRCC 2 cut(s) 247, 562
AccII CGCG 2 cut(s) 336, 338
AclWI GGATC 3 cut(s) 435, 448, 733
AcoI YGGCCR 1 cut(s) 517
AcyI GRCGYC 2 cut(s) 248, 496
AfiI CCNNNNNNNGG 7 cut(s) 157, 244, 261, 322, 485, 486, 686
AjnI CCWGG 5 cut(s) 155, 242, 320, 519, 576
AluBI AGCT 3 cut(s) 220, 377, 385
AluI AGCT 3 cut(s) 220, 377, 385
Alw21I GWGCWC 2 cut(s) 222, 387
Alw26I GTCTC 1 cut(s) 767
AlwI GGATC 3 cut(s) 435, 448, 733
Ama87I CYCGRG 2 cut(s) 261, 558
AoxI GGCC 6 cut(s) 66, 229, 394, 517, 555, 616
ApaI GGGCCC 1 cut(s) 70
ArsI GACNNNNNNTTYG 2 cut(s) 766, 798
AspA2I CCTAGG 2 cut(s) 451, 749
AspLEI GCGC 2 cut(s) 250, 338
AspS9I GGNCC 4 cut(s) 66, 67, 556, 617
AsuC2I CCSGG 5 cut(s) 90, 262, 263, 559, 560
AsuHPI GGTGA 5 cut(s) 17, 140, 305, 669, 823
AvaI CYCGRG 2 cut(s) 261, 558
AvrII CCTAGG 2 cut(s) 451, 749
AxyI CCTNAGG 1 cut(s) 484
BaeGI GKGCMC 3 cut(s) 70, 493, 565
BalI TGGCCA 1 cut(s) 519
BamHI GGATCC 1 cut(s) 440
BanI GGYRCC 2 cut(s) 247, 562
BanII GRGCYC 4 cut(s) 70, 222, 268, 387
BauI CACGAG 3 cut(s) 128, 168, 398
BbsI GAAGAC 2 cut(s) 491, 780
Bbv12I GWGCWC 2 cut(s) 222, 387
BccI CCATC 4 cut(s) 160, 325, 358, 705
BciT130I CCWGG 5 cut(s) 157, 244, 322, 521, 578
BcnI CCSGG 5 cut(s) 90, 262, 263, 559, 560
BcoDI GTCTC 1 cut(s) 767
BfaI CTAG 7 cut(s) 140, 305, 378, 404, 452, 669, 750
BfoI RGCGCY 1 cut(s) 251
BlnI CCTAGG 2 cut(s) 451, 749
BmeT110I CYCGRG 2 cut(s) 261, 558
BmgT120I GGNCC 4 cut(s) 66, 67, 556, 617
BmiI GGNNCC 7 cut(s) 67, 68, 249, 369, 442, 564, 619
BmrI ACTGGG 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 23
BpiI GAAGAC 2 cut(s) 491, 780
BpuMI CCSGG 5 cut(s) 90, 262, 263, 559, 560
BsaHI GRCGYC 2 cut(s) 248, 496
BsaJI CCNNGG 9 cut(s) 156, 243, 261, 321, 451, 520, 558, 576, 749
Bsc4I CCNNNNNNNGG 7 cut(s) 157, 244, 261, 322, 485, 486, 686
Bse1I ACTGG 2 cut(s) 18, 213
Bse21I CCTNAGG 1 cut(s) 484
BseBI CCWGG 5 cut(s) 157, 244, 322, 521, 578
BseDI CCNNGG 9 cut(s) 156, 243, 261, 321, 451, 520, 558, 576, 749
BseGI GGATG 7 cut(s) 85, 96, 152, 317, 470, 572, 768
BseLI CCNNNNNNNGG 7 cut(s) 157, 244, 261, 322, 485, 486, 686
BseMII CTCAG 1 cut(s) 721
BseNI ACTGG 2 cut(s) 18, 213
BseSI GKGCMC 3 cut(s) 70, 493, 565
Bsh1236I CGCG 2 cut(s) 336, 338
BshFI GGCC 6 cut(s) 68, 231, 396, 519, 557, 618
BshNI GGYRCC 2 cut(s) 247, 562
BsiHKAI GWGCWC 2 cut(s) 222, 387
BsiHKCI CYCGRG 2 cut(s) 261, 558
BsiSI CCGG 3 cut(s) 89, 262, 559
BslFI GGGAC 1 cut(s) 105
BslI CCNNNNNNNGG 7 cut(s) 157, 244, 261, 322, 485, 486, 686
BsmAI GTCTC 1 cut(s) 767
BsmFI GGGAC 1 cut(s) 105
BsmI GAATGC 1 cut(s) 696
BsnI GGCC 6 cut(s) 68, 231, 396, 519, 557, 618
BsoBI CYCGRG 2 cut(s) 261, 558
Bsp120I GGGCCC 1 cut(s) 66
Bsp1286I GDGCHC 6 cut(s) 70, 222, 268, 387, 493, 565
Bsp143I GATC 3 cut(s) 412, 440, 738
BspANI GGCC 6 cut(s) 68, 231, 396, 519, 557, 618
BspCNI CTCAG 1 cut(s) 720
BspFNI CGCG 2 cut(s) 336, 338
BspLI GGNNCC 7 cut(s) 67, 68, 249, 369, 442, 564, 619
BspPI GGATC 3 cut(s) 435, 448, 733
BspT107I GGYRCC 2 cut(s) 247, 562
BsrI ACTGG 2 cut(s) 18, 213
BssECI CCNNGG 9 cut(s) 156, 243, 261, 321, 451, 520, 558, 576, 749
BssMI GATC 3 cut(s) 412, 440, 738
BssNI GRCGYC 2 cut(s) 248, 496
BssSI CACGAG 3 cut(s) 128, 168, 398
BssT1I CCWWGG 2 cut(s) 451, 749
Bst2BI CACGAG 3 cut(s) 128, 168, 398
Bst2UI CCWGG 5 cut(s) 157, 244, 322, 521, 578
Bst4CI ACNGT 5 cut(s) 50, 107, 278, 593, 637
BstACI GRCGYC 2 cut(s) 248, 496
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 2 cut(s) 484, 707
BstEII GGTNACC 4 cut(s) 5, 146, 311, 675
BstF5I GGATG 7 cut(s) 85, 96, 152, 317, 470, 572, 768
BstFNI CGCG 2 cut(s) 336, 338
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 2 cut(s) 250, 338
BstKTI GATC 3 cut(s) 415, 443, 741
BstMAI GTCTC 1 cut(s) 767
BstMBI GATC 3 cut(s) 412, 440, 738
BstNI CCWGG 5 cut(s) 157, 244, 322, 521, 578
BstPI GGTNACC 4 cut(s) 5, 146, 311, 675
BstSLI GKGCMC 3 cut(s) 70, 493, 565
BstUI CGCG 2 cut(s) 336, 338
BstV2I GAAGAC 2 cut(s) 491, 780
BstX2I RGATCY 1 cut(s) 440
BstYI RGATCY 1 cut(s) 440
Bsu36I CCTNAGG 1 cut(s) 484
BsuRI GGCC 6 cut(s) 68, 231, 396, 519, 557, 618
BtgZI GCGATG 2 cut(s) 471, 769
BtsCI GGATG 7 cut(s) 85, 96, 152, 317, 470, 572, 768
BtsIMutI CAGTG 2 cut(s) 11, 220
Cac8I GCNNGC 1 cut(s) 222
CfoI GCGC 2 cut(s) 250, 338
Cfr13I GGNCC 4 cut(s) 66, 67, 556, 617
Cfr9I CCCGGG 2 cut(s) 261, 558
CviAII CATG 4 cut(s) 26, 598, 658, 736
DdeI CTNAG 2 cut(s) 484, 707
DinI GGCGCC 1 cut(s) 249
DpnI GATC 3 cut(s) 414, 442, 740
DpnII GATC 3 cut(s) 412, 440, 738
EaeI YGGCCR 1 cut(s) 517
Ecl136II GAGCTC 2 cut(s) 220, 385
Eco130I CCWWGG 2 cut(s) 451, 749
Eco147I AGGCCT 1 cut(s) 396
Eco24I GRGCYC 4 cut(s) 70, 222, 268, 387
Eco53kI GAGCTC 2 cut(s) 220, 385
Eco81I CCTNAGG 1 cut(s) 484
Eco88I CYCGRG 2 cut(s) 261, 558
Eco91I GGTNACC 4 cut(s) 5, 146, 311, 675
EcoICRI GAGCTC 2 cut(s) 220, 385
EcoO109I RGGNCCY 1 cut(s) 66
EcoO65I GGTNACC 4 cut(s) 5, 146, 311, 675
EcoRII CCWGG 5 cut(s) 155, 242, 320, 519, 576
EcoT14I CCWWGG 2 cut(s) 451, 749
EcoT38I GRGCYC 4 cut(s) 70, 222, 268, 387
EgeI GGCGCC 1 cut(s) 249
EheI GGCGCC 1 cut(s) 249
ErhI CCWWGG 2 cut(s) 451, 749
FaeI CATG 4 cut(s) 29, 601, 661, 739
FaqI GGGAC 1 cut(s) 105
FatI CATG 4 cut(s) 25, 597, 657, 735
FokI GGATG 7 cut(s) 72, 83, 139, 304, 477, 559, 775
FriOI GRGCYC 4 cut(s) 70, 222, 268, 387
FspBI CTAG 7 cut(s) 140, 305, 378, 404, 452, 669, 750
GlaI GCGC 2 cut(s) 249, 337
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 6 cut(s) 68, 231, 396, 519, 557, 618
HapII CCGG 3 cut(s) 89, 262, 559
HhaI GCGC 2 cut(s) 250, 338
Hin1I GRCGYC 2 cut(s) 248, 496
Hin1II CATG 4 cut(s) 29, 601, 661, 739
Hin6I GCGC 2 cut(s) 248, 336
HinP1I GCGC 2 cut(s) 248, 336
HinfI GANTC 2 cut(s) 270, 836
HpaII CCGG 3 cut(s) 89, 262, 559
HphI GGTGA 5 cut(s) 17, 140, 305, 669, 823
Hpy188I TCNGA 7 cut(s) 356, 363, 417, 534, 703, 710, 835
Hpy188III TCNNGA 1 cut(s) 238
Hpy99I CGWCG 1 cut(s) 498
HpyCH4III ACNGT 5 cut(s) 50, 107, 278, 593, 637
HpyCH4IV ACGT 1 cut(s) 496
HpyCH4V TGCA 2 cut(s) 173, 657
HpyF3I CTNAG 2 cut(s) 484, 707
HpySE526I ACGT 1 cut(s) 496
Hsp92I GRCGYC 2 cut(s) 248, 496
Hsp92II CATG 4 cut(s) 29, 601, 661, 739
HspAI GCGC 2 cut(s) 248, 336
KasI GGCGCC 1 cut(s) 247
Kzo9I GATC 3 cut(s) 412, 440, 738
LmnI GCTCC 1 cut(s) 390
MaeI CTAG 7 cut(s) 140, 305, 378, 404, 452, 669, 750
MaeII ACGT 1 cut(s) 496
MaeIII GTNAC 8 cut(s) 5, 119, 146, 311, 467, 545, 675, 765
MalI GATC 3 cut(s) 414, 442, 740
MboI GATC 3 cut(s) 412, 440, 738
MboII GAAGA 2 cut(s) 491, 780
MflI RGATCY 1 cut(s) 440
MhlI GDGCHC 6 cut(s) 70, 222, 268, 387, 493, 565
MlsI TGGCCA 1 cut(s) 519
MluCI AATT 1 cut(s) 541
MluNI TGGCCA 1 cut(s) 519
Mly113I GGCGCC 1 cut(s) 248
MlyI GAGTC 2 cut(s) 264, 830
MnlI CCTC 4 cut(s) 46, 111, 407, 641
Mox20I TGGCCA 1 cut(s) 519
MscI TGGCCA 1 cut(s) 519
MseI TTAA 3 cut(s) 183, 348, 794
Msp20I TGGCCA 1 cut(s) 519
MspI CCGG 3 cut(s) 89, 262, 559
Mva1269I GAATGC 1 cut(s) 696
MvaI CCWGG 5 cut(s) 157, 244, 322, 521, 578
MvnI CGCG 2 cut(s) 336, 338
NarI GGCGCC 1 cut(s) 248
NciI CCSGG 5 cut(s) 90, 262, 263, 559, 560
NdeII GATC 3 cut(s) 412, 440, 738
NlaIII CATG 4 cut(s) 29, 601, 661, 739
NlaIV GGNNCC 7 cut(s) 67, 68, 249, 369, 442, 564, 619
NmeAIII GCCGAG 1 cut(s) 533
NmuCI GTSAC 5 cut(s) 5, 146, 311, 545, 675
PceI AGGCCT 1 cut(s) 396
PcsI WCGNNNNNNNCGW 1 cut(s) 274
PctI GAATGC 1 cut(s) 696
PfoI TCCNGGA 1 cut(s) 88
PleI GAGTC 2 cut(s) 264, 830
PluTI GGCGCC 1 cut(s) 251
PpsI GAGTC 2 cut(s) 264, 830
Psp124BI GAGCTC 2 cut(s) 222, 387
Psp6I CCWGG 5 cut(s) 155, 242, 320, 519, 576
PspEI GGTNACC 4 cut(s) 5, 146, 311, 675
PspGI CCWGG 5 cut(s) 155, 242, 320, 519, 576
PspN4I GGNNCC 7 cut(s) 67, 68, 249, 369, 442, 564, 619
PspOMI GGGCCC 1 cut(s) 66
PspPI GGNCC 4 cut(s) 66, 67, 556, 617
PsuI RGATCY 1 cut(s) 440
SacI GAGCTC 2 cut(s) 222, 387
SaqAI TTAA 3 cut(s) 183, 348, 794
Sau3AI GATC 3 cut(s) 412, 440, 738
Sau96I GGNCC 4 cut(s) 66, 67, 556, 617
SchI GAGTC 2 cut(s) 264, 830
SduI GDGCHC 6 cut(s) 70, 222, 268, 387, 493, 565
SetI ASST 8 cut(s) 222, 245, 313, 379, 387, 409, 485, 499
SfoI GGCGCC 1 cut(s) 249
SmaI CCCGGG 2 cut(s) 263, 560
SrfI GCCCGGGC 1 cut(s) 560
Sse9I AATT 1 cut(s) 541
SseBI AGGCCT 1 cut(s) 396
SspDI GGCGCC 1 cut(s) 247
SspMI CTAG 7 cut(s) 140, 305, 378, 404, 452, 669, 750
SstI GAGCTC 2 cut(s) 222, 387
StuI AGGCCT 1 cut(s) 396
StyI CCWWGG 2 cut(s) 451, 749
TaaI ACNGT 5 cut(s) 50, 107, 278, 593, 637
TaiI ACGT 1 cut(s) 499
TaqI TCGA 1 cut(s) 268
TasI AATT 1 cut(s) 541
Tru1I TTAA 3 cut(s) 183, 348, 794
Tru9I TTAA 3 cut(s) 183, 348, 794
TscAI CASTG 2 cut(s) 18, 220
TseFI GTSAC 5 cut(s) 5, 146, 311, 545, 675
Tsp45I GTSAC 5 cut(s) 5, 146, 311, 545, 675
TspDTI ATGAA 2 cut(s) 14, 491
TspGWI ACGGA 2 cut(s) 215, 598
TspMI CCCGGG 2 cut(s) 261, 558
TspRI CASTG 2 cut(s) 18, 220
XmaI CCCGGG 2 cut(s) 261, 558
XmaJI CCTAGG 2 cut(s) 451, 749
XspI CTAG 7 cut(s) 140, 305, 378, 404, 452, 669, 750
ZraI GACGTC 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.