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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1802 |
| AccBSI | CCGCTC | 1 cut(s) | 1698 |
| AccII | CGCG | 1 cut(s) | 672 |
| AciI | CCGC | 3 cut(s) | 539, 762, 1696 |
| AclWI | GGATC | 6 cut(s) | 151, 395, 408, 599, 612, 1009 |
| AcoI | YGGCCR | 1 cut(s) | 573 |
| AcsI | RAATTY | 5 cut(s) | 718, 1342, 1424, 1650, 1870 |
| AfaI | GTAC | 2 cut(s) | 1061, 1608 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 689, 817, 1449, 1707 |
| AgsI | TTSAA | 6 cut(s) | 122, 490, 1186, 1270, 1430, 1989 |
| AjnI | CCWGG | 3 cut(s) | 389, 570, 700 |
| AluBI | AGCT | 7 cut(s) | 459, 622, 792, 816, 1261, 1562, 1602 |
| AluI | AGCT | 7 cut(s) | 459, 622, 792, 816, 1261, 1562, 1602 |
| Alw21I | GWGCWC | 4 cut(s) | 461, 624, 794, 1564 |
| Alw26I | GTCTC | 1 cut(s) | 1010 |
| AlwI | GGATC | 6 cut(s) | 151, 395, 408, 599, 612, 1009 |
| Ama87I | CYCGRG | 2 cut(s) | 793, 811 |
| AoxI | GGCC | 4 cut(s) | 446, 573, 753, 808 |
| ApaI | GGGCCC | 2 cut(s) | 757, 812 |
| ApeKI | GCWGC | 2 cut(s) | 1687, 1997 |
| ApoI | RAATTY | 5 cut(s) | 718, 1342, 1424, 1650, 1870 |
| AseI | ATTAAT | 1 cut(s) | 1319 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 42, 1150, 1417 |
| AspLEI | GCGC | 1 cut(s) | 672 |
| AspS9I | GGNCC | 6 cut(s) | 594, 753, 754, 808, 809, 898 |
| AsuHPI | GGTGA | 1 cut(s) | 704 |
| AsuII | TTCGAA | 1 cut(s) | 93 |
| AvaI | CYCGRG | 2 cut(s) | 793, 811 |
| AvaII | GGWCC | 2 cut(s) | 594, 898 |
| BaeGI | GKGCMC | 3 cut(s) | 277, 757, 812 |
| BamHI | GGATCC | 2 cut(s) | 400, 604 |
| BanII | GRGCYC | 6 cut(s) | 461, 624, 757, 794, 812, 1564 |
| BauI | CACGAG | 1 cut(s) | 711 |
| BbsI | GAAGAC | 3 cut(s) | 952, 1437, 1500 |
| Bbv12I | GWGCWC | 4 cut(s) | 461, 624, 794, 1564 |
| BbvI | GCAGC | 2 cut(s) | 1699, 1984 |
| BccI | CCATC | 3 cut(s) | 485, 648, 682 |
| BceAI | ACGGC | 1 cut(s) | 1070 |
| BciT130I | CCWGG | 3 cut(s) | 391, 572, 702 |
| BclI | TGATCA | 3 cut(s) | 82, 1390, 1531 |
| BcoDI | GTCTC | 1 cut(s) | 1010 |
| BfaI | CTAG | 7 cut(s) | 149, 270, 438, 1034, 1071, 1410, 1647 |
| BfmI | CTRYAG | 1 cut(s) | 1998 |
| BglII | AGATCT | 1 cut(s) | 1139 |
| BisI | GCNGC | 2 cut(s) | 1688, 1998 |
| BlsI | GCNGC | 2 cut(s) | 1689, 1999 |
| Bme1390I | CCNGG | 3 cut(s) | 391, 572, 702 |
| Bme18I | GGWCC | 2 cut(s) | 594, 898 |
| BmeT110I | CYCGRG | 2 cut(s) | 793, 811 |
| BmgT120I | GGNCC | 6 cut(s) | 594, 753, 754, 808, 809, 898 |
| BmrFI | CCNGG | 3 cut(s) | 391, 572, 702 |
| BmrI | ACTGGG | 1 cut(s) | 1084 |
| BmsI | GCATC | 1 cut(s) | 26 |
| BmuI | ACTGGG | 1 cut(s) | 1084 |
| BpiI | GAAGAC | 3 cut(s) | 952, 1437, 1500 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 776, 808 |
| Bpu14I | TTCGAA | 1 cut(s) | 93 |
| BsaJI | CCNNGG | 3 cut(s) | 389, 390, 570 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 689, 817, 1449, 1707 |
| Bse1I | ACTGG | 3 cut(s) | 631, 1052, 1090 |
| BseBI | CCWGG | 3 cut(s) | 391, 572, 702 |
| BseDI | CCNNGG | 3 cut(s) | 389, 390, 570 |
| BseGI | GGATG | 4 cut(s) | 17, 803, 826, 1928 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 689, 817, 1449, 1707 |
| BseNI | ACTGG | 3 cut(s) | 631, 1052, 1090 |
| BseSI | GKGCMC | 3 cut(s) | 277, 757, 812 |
| BseXI | GCAGC | 2 cut(s) | 1699, 1984 |
| BseYI | CCCAGC | 1 cut(s) | 816 |
| Bsh1236I | CGCG | 1 cut(s) | 672 |
| BshFI | GGCC | 4 cut(s) | 448, 575, 755, 810 |
| BsiHKAI | GWGCWC | 4 cut(s) | 461, 624, 794, 1564 |
| BsiHKCI | CYCGRG | 2 cut(s) | 793, 811 |
| BsiSI | CCGG | 1 cut(s) | 576 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 689, 817, 1449, 1707 |
| BsmAI | GTCTC | 1 cut(s) | 1010 |
| BsmBI | CGTCTC | 1 cut(s) | 1010 |
| BsnI | GGCC | 4 cut(s) | 448, 575, 755, 810 |
| BsoBI | CYCGRG | 2 cut(s) | 793, 811 |
| Bsp119I | TTCGAA | 1 cut(s) | 93 |
| Bsp120I | GGGCCC | 2 cut(s) | 753, 808 |
| Bsp1286I | GDGCHC | 7 cut(s) | 277, 461, 624, 757, 794, 812, 1564 |
| Bsp1407I | TGTACA | 1 cut(s) | 1606 |
| Bsp143I | GATC | 8 cut(s) | 82, 156, 400, 604, 1001, 1139, 1390, 1531 |
| BspACI | CCGC | 3 cut(s) | 539, 762, 1696 |
| BspANI | GGCC | 4 cut(s) | 448, 575, 755, 810 |
| BspFNI | CGCG | 1 cut(s) | 672 |
| BspMAI | CTGCAG | 1 cut(s) | 2002 |
| BspPI | GGATC | 6 cut(s) | 151, 395, 408, 599, 612, 1009 |
| BspQI | GCTCTTC | 1 cut(s) | 173 |
| BspT104I | TTCGAA | 1 cut(s) | 93 |
| BsrBI | CCGCTC | 1 cut(s) | 1698 |
| BsrGI | TGTACA | 1 cut(s) | 1606 |
| BsrI | ACTGG | 3 cut(s) | 631, 1052, 1090 |
| BssECI | CCNNGG | 3 cut(s) | 389, 390, 570 |
| BssMI | GATC | 8 cut(s) | 82, 156, 400, 604, 1001, 1139, 1390, 1531 |
| BssSI | CACGAG | 1 cut(s) | 711 |
| Bst2BI | CACGAG | 1 cut(s) | 711 |
| Bst2UI | CCWGG | 3 cut(s) | 391, 572, 702 |
| Bst4CI | ACNGT | 5 cut(s) | 514, 558, 601, 737, 781 |
| Bst6I | CTCTTC | 1 cut(s) | 173 |
| BstAUI | TGTACA | 1 cut(s) | 1606 |
| BstBI | TTCGAA | 1 cut(s) | 93 |
| BstC8I | GCNNGC | 1 cut(s) | 1382 |
| BstDEI | CTNAG | 4 cut(s) | 9, 132, 1556, 1815 |
| BstEII | GGTNACC | 1 cut(s) | 692 |
| BstF5I | GGATG | 4 cut(s) | 17, 803, 826, 1928 |
| BstFNI | CGCG | 1 cut(s) | 672 |
| BstHHI | GCGC | 1 cut(s) | 672 |
| BstKTI | GATC | 8 cut(s) | 85, 159, 403, 607, 1004, 1142, 1393, 1534 |
| BstMAI | GTCTC | 1 cut(s) | 1010 |
| BstMBI | GATC | 8 cut(s) | 82, 156, 400, 604, 1001, 1139, 1390, 1531 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 385, 667 |
| BstNI | CCWGG | 3 cut(s) | 391, 572, 702 |
| BstNSI | RCATGY | 4 cut(s) | 1596, 1629, 1749, 1948 |
| BstPI | GGTNACC | 1 cut(s) | 692 |
| BstSCI | CCNGG | 3 cut(s) | 389, 570, 700 |
| BstSFI | CTRYAG | 1 cut(s) | 1998 |
| BstSLI | GKGCMC | 3 cut(s) | 277, 757, 812 |
| BstUI | CGCG | 1 cut(s) | 672 |
| BstV1I | GCAGC | 2 cut(s) | 1699, 1984 |
| BstV2I | GAAGAC | 3 cut(s) | 952, 1437, 1500 |
| BstX2I | RGATCY | 4 cut(s) | 156, 400, 604, 1139 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 188, 397, 976 |
| BstYI | RGATCY | 4 cut(s) | 156, 400, 604, 1139 |
| BsuRI | GGCC | 4 cut(s) | 448, 575, 755, 810 |
| BtgZI | GCGATG | 1 cut(s) | 369 |
| BtsCI | GGATG | 4 cut(s) | 17, 803, 826, 1928 |
| BtsI | GCAGTG | 1 cut(s) | 321 |
| BtsIMutI | CAGTG | 3 cut(s) | 321, 606, 624 |
| Cac8I | GCNNGC | 1 cut(s) | 1382 |
| CfoI | GCGC | 1 cut(s) | 672 |
| Cfr13I | GGNCC | 6 cut(s) | 594, 753, 754, 808, 809, 898 |
| Csp6I | GTAC | 2 cut(s) | 1060, 1607 |
| CviQI | GTAC | 2 cut(s) | 1060, 1607 |
| DdeI | CTNAG | 4 cut(s) | 9, 132, 1556, 1815 |
| DpnI | GATC | 8 cut(s) | 84, 158, 402, 606, 1003, 1141, 1392, 1533 |
| DpnII | GATC | 8 cut(s) | 82, 156, 400, 604, 1001, 1139, 1390, 1531 |
| DraI | TTTAAA | 2 cut(s) | 114, 951 |
| EaeI | YGGCCR | 1 cut(s) | 573 |
| Eam1104I | CTCTTC | 1 cut(s) | 173 |
| EarI | CTCTTC | 1 cut(s) | 173 |
| Ecl136II | GAGCTC | 4 cut(s) | 459, 622, 792, 1562 |
| Eco147I | AGGCCT | 1 cut(s) | 448 |
| Eco24I | GRGCYC | 6 cut(s) | 461, 624, 757, 794, 812, 1564 |
| Eco32I | GATATC | 1 cut(s) | 1975 |
| Eco47I | GGWCC | 2 cut(s) | 594, 898 |
| Eco53kI | GAGCTC | 4 cut(s) | 459, 622, 792, 1562 |
| Eco88I | CYCGRG | 2 cut(s) | 793, 811 |
| Eco91I | GGTNACC | 1 cut(s) | 692 |
| EcoICRI | GAGCTC | 4 cut(s) | 459, 622, 792, 1562 |
| EcoO109I | RGGNCCY | 2 cut(s) | 753, 808 |
| EcoO65I | GGTNACC | 1 cut(s) | 692 |
| EcoRI | GAATTC | 1 cut(s) | 718 |
| EcoRII | CCWGG | 3 cut(s) | 389, 570, 700 |
| EcoRV | GATATC | 1 cut(s) | 1975 |
| EcoT22I | ATGCAT | 2 cut(s) | 1594, 1631 |
| EcoT38I | GRGCYC | 6 cut(s) | 461, 624, 757, 794, 812, 1564 |
| Esp3I | CGTCTC | 1 cut(s) | 1010 |
| FauNDI | CATATG | 2 cut(s) | 79, 1979 |
| FbaI | TGATCA | 3 cut(s) | 82, 1390, 1531 |
| Fnu4HI | GCNGC | 2 cut(s) | 1688, 1998 |
| FokI | GGATG | 4 cut(s) | 4, 810, 833, 1915 |
| FriOI | GRGCYC | 6 cut(s) | 461, 624, 757, 794, 812, 1564 |
| Fsp4HI | GCNGC | 2 cut(s) | 1688, 1998 |
| FspBI | CTAG | 7 cut(s) | 149, 270, 438, 1034, 1071, 1410, 1647 |
| GlaI | GCGC | 1 cut(s) | 671 |
| GluI | GCNGC | 2 cut(s) | 1688, 1998 |
| GsaI | CCCAGC | 1 cut(s) | 820 |
| HaeIII | GGCC | 4 cut(s) | 448, 575, 755, 810 |
| HapII | CCGG | 1 cut(s) | 576 |
| HhaI | GCGC | 1 cut(s) | 672 |
| Hin6I | GCGC | 1 cut(s) | 670 |
| HinP1I | GCGC | 1 cut(s) | 670 |
| HinfI | GANTC | 3 cut(s) | 325, 562, 1041 |
| HpaII | CCGG | 1 cut(s) | 576 |
| HphI | GGTGA | 1 cut(s) | 704 |
| Hpy166II | GTNNAC | 2 cut(s) | 1609, 1618 |
| Hpy188I | TCNGA | 5 cut(s) | 482, 529, 645, 652, 1423 |
| Hpy188III | TCNNGA | 6 cut(s) | 795, 1143, 1410, 1443, 1490, 1647 |
| Hpy8I | GTNNAC | 2 cut(s) | 1609, 1618 |
| Hpy99I | CGWCG | 1 cut(s) | 592 |
| HpyCH4III | ACNGT | 5 cut(s) | 514, 558, 601, 737, 781 |
| HpyCH4IV | ACGT | 2 cut(s) | 1019, 1658 |
| HpyCH4V | TGCA | 7 cut(s) | 17, 314, 336, 1592, 1629, 1944, 2000 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 385, 667 |
| HpyF3I | CTNAG | 4 cut(s) | 9, 132, 1556, 1815 |
| HpySE526I | ACGT | 2 cut(s) | 1019, 1658 |
| HspAI | GCGC | 1 cut(s) | 670 |
| Ksp22I | TGATCA | 3 cut(s) | 82, 1390, 1531 |
| Kzo9I | GATC | 8 cut(s) | 82, 156, 400, 604, 1001, 1139, 1390, 1531 |
| LguI | GCTCTTC | 1 cut(s) | 173 |
| LmnI | GCTCC | 7 cut(s) | 456, 529, 619, 857, 867, 1091, 1703 |
| Lsp1109I | GCAGC | 2 cut(s) | 1699, 1984 |
| LweI | GCATC | 1 cut(s) | 26 |
| MaeI | CTAG | 7 cut(s) | 149, 270, 438, 1034, 1071, 1410, 1647 |
| MaeII | ACGT | 2 cut(s) | 1019, 1658 |
| MaeIII | GTNAC | 5 cut(s) | 692, 823, 1020, 1226, 1741 |
| MalI | GATC | 8 cut(s) | 84, 158, 402, 606, 1003, 1141, 1392, 1533 |
| MbiI | CCGCTC | 1 cut(s) | 1698 |
| MboI | GATC | 8 cut(s) | 82, 156, 400, 604, 1001, 1139, 1390, 1531 |
| MboII | GAAGA | 5 cut(s) | 160, 952, 1146, 1442, 1505 |
| MfeI | CAATTG | 1 cut(s) | 893 |
| MflI | RGATCY | 4 cut(s) | 156, 400, 604, 1139 |
| MhlI | GDGCHC | 7 cut(s) | 277, 461, 624, 757, 794, 812, 1564 |
| MlyI | GAGTC | 1 cut(s) | 571 |
| MmeI | TCCRAC | 3 cut(s) | 336, 507, 1305 |
| MnlI | CCTC | 8 cut(s) | 112, 187, 207, 339, 438, 510, 585, 733 |
| Mph1103I | ATGCAT | 2 cut(s) | 1594, 1631 |
| MroXI | GAANNNNTTC | 3 cut(s) | 42, 1150, 1417 |
| MspI | CCGG | 1 cut(s) | 576 |
| MspR9I | CCNGG | 3 cut(s) | 391, 572, 702 |
| MunI | CAATTG | 1 cut(s) | 893 |
| MvaI | CCWGG | 3 cut(s) | 391, 572, 702 |
| MvnI | CGCG | 1 cut(s) | 672 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 385, 667 |
| NdeI | CATATG | 2 cut(s) | 79, 1979 |
| NdeII | GATC | 8 cut(s) | 82, 156, 400, 604, 1001, 1139, 1390, 1531 |
| NmuCI | GTSAC | 2 cut(s) | 692, 823 |
| NsiI | ATGCAT | 2 cut(s) | 1594, 1631 |
| NspI | RCATGY | 4 cut(s) | 1596, 1629, 1749, 1948 |
| NspV | TTCGAA | 1 cut(s) | 93 |
| PasI | CCCWGGG | 1 cut(s) | 390 |
| PceI | AGGCCT | 1 cut(s) | 448 |
| PciSI | GCTCTTC | 1 cut(s) | 173 |
| PdmI | GAANNNNTTC | 3 cut(s) | 42, 1150, 1417 |
| PfeI | GAWTC | 2 cut(s) | 325, 1041 |
| PkrI | GCNGC | 2 cut(s) | 1689, 1999 |
| PleI | GAGTC | 1 cut(s) | 570 |
| PpsI | GAGTC | 1 cut(s) | 570 |
| PshBI | ATTAAT | 1 cut(s) | 1319 |
| PsiI | TTATAA | 1 cut(s) | 1802 |
| Psp124BI | GAGCTC | 4 cut(s) | 461, 624, 794, 1564 |
| Psp6I | CCWGG | 3 cut(s) | 389, 570, 700 |
| PspEI | GGTNACC | 1 cut(s) | 692 |
| PspFI | CCCAGC | 1 cut(s) | 816 |
| PspGI | CCWGG | 3 cut(s) | 389, 570, 700 |
| PspOMI | GGGCCC | 2 cut(s) | 753, 808 |
| PspPI | GGNCC | 6 cut(s) | 594, 753, 754, 808, 809, 898 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1599, 1631 |
| PstI | CTGCAG | 1 cut(s) | 2002 |
| PsuI | RGATCY | 4 cut(s) | 156, 400, 604, 1139 |
| RsaI | GTAC | 2 cut(s) | 1061, 1608 |
| RsaNI | GTAC | 2 cut(s) | 1060, 1607 |
| SacI | GAGCTC | 4 cut(s) | 461, 624, 794, 1564 |
| SapI | GCTCTTC | 1 cut(s) | 173 |
| SatI | GCNGC | 2 cut(s) | 1688, 1998 |
| Sau3AI | GATC | 8 cut(s) | 82, 156, 400, 604, 1001, 1139, 1390, 1531 |
| Sau96I | GGNCC | 6 cut(s) | 594, 753, 754, 808, 809, 898 |
| SchI | GAGTC | 1 cut(s) | 571 |
| ScrFI | CCNGG | 3 cut(s) | 391, 572, 702 |
| SduI | GDGCHC | 7 cut(s) | 277, 461, 624, 757, 794, 812, 1564 |
| SfaNI | GCATC | 1 cut(s) | 26 |
| SfcI | CTRYAG | 1 cut(s) | 1998 |
| SfuI | TTCGAA | 1 cut(s) | 93 |
| SinI | GGWCC | 2 cut(s) | 594, 898 |
| SseBI | AGGCCT | 1 cut(s) | 448 |
| SsiI | CCGC | 3 cut(s) | 539, 762, 1696 |
| SspI | AATATT | 1 cut(s) | 1149 |
| SspMI | CTAG | 7 cut(s) | 149, 270, 438, 1034, 1071, 1410, 1647 |
| SstI | GAGCTC | 4 cut(s) | 461, 624, 794, 1564 |
| StuI | AGGCCT | 1 cut(s) | 448 |
| StyD4I | CCNGG | 3 cut(s) | 389, 570, 700 |
| TaaI | ACNGT | 5 cut(s) | 514, 558, 601, 737, 781 |
| TaiI | ACGT | 2 cut(s) | 1022, 1661 |
| TaqI | TCGA | 2 cut(s) | 93, 1144 |
| TatI | WGTACW | 2 cut(s) | 1059, 1606 |
| TfiI | GAWTC | 2 cut(s) | 325, 1041 |
| TscAI | CASTG | 3 cut(s) | 321, 606, 631 |
| TseFI | GTSAC | 2 cut(s) | 692, 823 |
| TseI | GCWGC | 2 cut(s) | 1687, 1997 |
| Tsp45I | GTSAC | 2 cut(s) | 692, 823 |
| TspDTI | ATGAA | 9 cut(s) | 17, 1355, 1390, 1475, 1573, 1628, 1692, 1893, 1917 |
| TspRI | CASTG | 3 cut(s) | 321, 606, 631 |
| VpaK11BI | GGWCC | 2 cut(s) | 594, 898 |
| VspI | ATTAAT | 1 cut(s) | 1319 |
| XapI | RAATTY | 5 cut(s) | 718, 1342, 1424, 1650, 1870 |
| XbaI | TCTAGA | 2 cut(s) | 1409, 1646 |
| XceI | RCATGY | 4 cut(s) | 1596, 1629, 1749, 1948 |
| XmnI | GAANNNNTTC | 3 cut(s) | 42, 1150, 1417 |
| XspI | CTAG | 7 cut(s) | 149, 270, 438, 1034, 1071, 1410, 1647 |
| Zsp2I | ATGCAT | 2 cut(s) | 1594, 1631 |